AT5G16050

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009784
Physical Location & Seq
Reverse (-)
5243555 .. 5245838
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16050.2

Sequence Viewer

Length: 807 bp
ATGTCTTCTGATTCGTCCCGGGAAGAGAATGTGTACTTGGCCAAGTTAGCCGAGCAAGCTGAGCGTTACGAGGAAATGGTTGAGTTCATGGAGAAAGTTGCAAAGACCGTGGAGACCGAGGAACTTACTGTTGAAGAGAGGAATCTCTTGTCTGTTGCTTACAAGAACGTGATTGGTGCTAGGAGAGCTTCTTGGAGGATTATCTCTTCCATTGAGCAGAAGGAAGATAGCAGGGGCAACAGTGATCATGTTTCGATTATCAAGGATTACAGAGGCAAGATTGAAACTGAGCTCAGCAAGATTTGTGATGGCATTTTGAACCTTCTTGAGGCTCATCTCATTCCTGCTGCTTCTTTGGCTGAGTCCAAAGTTTTTTACCTGAAGATGAAGGGAGATTATCATCGGTACCTTGCTGAATTCAAGACTGGTGCTGAGAGGAAAGAAGCTGCTGAGAGCACTCTTGTTGCCTACAAGTCTGCTCAGGATATTGCTCTTGCTGATCTGGCTCCCACTCACCCAATCAGACTGGGGCTTGCTCTTAACTTCTCTGTTTTCTACTATGAGATTCTCAACTCATCTGATCGTGCGTGTAGTCTCGCAAAGCAGGCTTTTGATGAGGCAATCTCGGAGCTAGACACATTGGGAGAGGAATCATACAAGGACAGTACATTGATCATGCAGCTTCTCCGTGACAATCTCACCCTCTGGACTTCTGACCTCAATGACGAAGCTGGTGATGATATCAAGGAAGCCCCGAAAGAGGTGCAGAAAGTTGATGAACAAGCCCAACCACCACCTTCGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000166 GO:0001505 GO:0001932 GO:0001933 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004620 GO:0004623 GO:0004857 GO:0004860 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006469 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006587 GO:0006588 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008144 GO:0008150 GO:0008152 GO:0008426 GO:0009058 GO:0009072 GO:0009308 GO:0009506 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010563 GO:0010605 GO:0012505 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0017076 GO:0018130 GO:0018958 GO:0019207 GO:0019210 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0019887 GO:0030054 GO:0030234 GO:0030291 GO:0030312 GO:0030554 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031570 GO:0032268 GO:0032269 GO:0032553 GO:0032555 GO:0032559 GO:0032768 GO:0032770 GO:0033554 GO:0033673 GO:0034641 GO:0035639 GO:0036094 GO:0042133 GO:0042136 GO:0042221 GO:0042325 GO:0042326 GO:0042427 GO:0042428 GO:0042430 GO:0042435 GO:0043085 GO:0043086 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043549 GO:0044092 GO:0044093 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045786 GO:0045859 GO:0045936 GO:0046189 GO:0046483 GO:0046686 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051341 GO:0051348 GO:0051353 GO:0051716 GO:0051726 GO:0052689 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0097159 GO:0097164 GO:0097367 GO:0098772 GO:1901160 GO:1901162 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901615 GO:1901617
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04142 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04142 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

268

Amino Acids

30.18

Weight (kDa)

4.73

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 237 1.3e-103 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009784)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02520 AT3G02520 AT5G16050 AT5G16050 AT5G38480 AT5G38480 AT5G38480
fragaria_vesca FvH4_6g45301
malus_domestica MD07G1096000.v1.1 MD17G1074900.v1.1 MD17G1105100.v1.1
prunus_persica Prupe.3G239000_v2.0.a1
pyrus_communis pycom09g01110
rosa_chinensis RchiOBHm_Chr2g0163061
rosa_laevigata RLG00000021368
rosa_multiflora Rmu_sc0003984.1_g000004
rosa_roxburghii Rroxscaffold_2G00087580
rosa_rugosa Rorug02G0500100
rosa_samantha Rh2AG566400 Rh2BG578800
rosa_wichuraiana Rw2G046890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 405
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 3 cut(s) 35, 407, 667
AfiI CCNNNNNNNGG 2 cut(s) 328, 760
AgsI TTSAA 4 cut(s) 134, 284, 319, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 7 cut(s) 59, 188, 292, 446, 631, 682, 731
AluI AGCT 7 cut(s) 59, 188, 292, 446, 631, 682, 731
Alw21I GWGCWC 2 cut(s) 294, 458
Alw26I GTCTC 2 cut(s) 107, 599
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 347, 446, 679
ApoI RAATTY 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 405
AsuC2I CCSGG 2 cut(s) 19, 20
AsuHPI GGTGA 3 cut(s) 506, 691, 746
AvaI CYCGRG 1 cut(s) 18
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 294, 458
BbvI GCAGC 3 cut(s) 334, 433, 691
BccI CCATC 1 cut(s) 302
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BclI TGATCA 2 cut(s) 244, 672
BcnI CCSGG 2 cut(s) 19, 20
BcoDI GTCTC 2 cut(s) 107, 599
BfaI CTAG 2 cut(s) 180, 632
BisI GCNGC 3 cut(s) 348, 447, 680
BlpI GCTNAGC 2 cut(s) 60, 293
BlsI GCNGC 3 cut(s) 349, 448, 681
Bme1390I CCNGG 2 cut(s) 19, 20
BmeT110I CYCGRG 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 407, 507
BmrFI CCNGG 2 cut(s) 19, 20
BmrI ACTGGG 1 cut(s) 536
BmuI ACTGGG 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 608, 640
Bpu10I CCTNAGC 1 cut(s) 480
Bpu1102I GCTNAGC 2 cut(s) 60, 293
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 2 cut(s) 19, 20
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 18, 108, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 760
Bse1I ACTGG 2 cut(s) 430, 531
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 18, 108, 117
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 328, 760
BseMII CTCAG 7 cut(s) 51, 279, 307, 351, 423, 441, 494
BseNI ACTGG 2 cut(s) 430, 531
BseXI GCAGC 3 cut(s) 334, 433, 691
BsgI GTGCAG 1 cut(s) 785
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 294, 458
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 328, 760
BsmAI GTCTC 2 cut(s) 107, 599
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 294, 458
Bsp143I GATC 4 cut(s) 244, 499, 580, 672
Bsp1720I GCTNAGC 2 cut(s) 60, 293
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 7 cut(s) 52, 280, 306, 352, 424, 442, 493
BspLI GGNNCC 2 cut(s) 407, 507
BspT107I GGYRCC 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 2 cut(s) 430, 531
BssECI CCNNGG 3 cut(s) 18, 108, 117
BssMI GATC 4 cut(s) 244, 499, 580, 672
Bst4CI ACNGT 4 cut(s) 109, 130, 242, 665
Bst6I CTCTTC 3 cut(s) 18, 129, 211
BstC8I GCNNGC 3 cut(s) 57, 534, 606
BstDEI CTNAG 7 cut(s) 60, 288, 293, 360, 432, 450, 480
BstDSI CCRYGG 1 cut(s) 108
BstENI CCTNNNNNAGG 1 cut(s) 326
BstKTI GATC 4 cut(s) 247, 502, 583, 675
BstMAI GTCTC 2 cut(s) 107, 599
BstMBI GATC 4 cut(s) 244, 499, 580, 672
BstMWI GCNNNNNNNGC 7 cut(s) 47, 56, 61, 185, 356, 503, 605
BstSCI CCNGG 2 cut(s) 17, 18
BstV1I GCAGC 3 cut(s) 334, 433, 691
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 57, 534, 606
Cfr9I CCCGGG 1 cut(s) 18
Csp6I GTAC 3 cut(s) 34, 406, 666
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviAII CATG 3 cut(s) 88, 248, 676
CviQI GTAC 3 cut(s) 34, 406, 666
DdeI CTNAG 7 cut(s) 60, 288, 293, 360, 432, 450, 480
DpnI GATC 4 cut(s) 246, 501, 582, 674
DpnII GATC 4 cut(s) 244, 499, 580, 672
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 18, 129, 211
EarI CTCTTC 3 cut(s) 18, 129, 211
Ecl136II GAGCTC 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 742
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 416
EcoRV GATATC 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 294
FaeI CATG 3 cut(s) 91, 251, 679
FaiI YATR 5 cut(s) 89, 249, 561, 655, 677
FatI CATG 3 cut(s) 87, 247, 675
FbaI TGATCA 2 cut(s) 244, 672
Fnu4HI GCNGC 3 cut(s) 348, 447, 680
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 348, 447, 680
FspBI CTAG 2 cut(s) 180, 632
GluI GCNGC 3 cut(s) 348, 447, 680
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 19
Hin1II CATG 3 cut(s) 91, 251, 679
HinfI GANTC 5 cut(s) 11, 142, 362, 565, 650
HpaII CCGG 1 cut(s) 19
HphI GGTGA 3 cut(s) 506, 691, 746
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 5 cut(s) 10, 524, 580, 628, 715
Hpy188III TCNNGA 4 cut(s) 326, 421, 482, 706
Hpy8I GTNNAC 1 cut(s) 34
HpyAV CCTTC 4 cut(s) 214, 332, 382, 807
HpyCH4III ACNGT 4 cut(s) 109, 130, 242, 665
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 3 cut(s) 101, 679, 766
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 56, 61, 185, 356, 503, 605
HpyF3I CTNAG 7 cut(s) 60, 288, 293, 360, 432, 450, 480
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 3 cut(s) 91, 251, 679
KpnI GGTACC 1 cut(s) 409
Ksp22I TGATCA 2 cut(s) 244, 672
Kzo9I GATC 4 cut(s) 244, 499, 580, 672
LmnI GCTCC 2 cut(s) 511, 628
Lsp1109I GCAGC 3 cut(s) 334, 433, 691
MaeI CTAG 2 cut(s) 180, 632
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 65, 689
MalI GATC 4 cut(s) 246, 501, 582, 674
MboI GATC 4 cut(s) 244, 499, 580, 672
MboII GAAGA 5 cut(s) 35, 146, 198, 236, 394
MhlI GDGCHC 2 cut(s) 294, 458
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 371
Mox20I TGGCCA 1 cut(s) 41
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 1 cut(s) 19
MspR9I CCNGG 2 cut(s) 19, 20
MwoI GCNNNNNNNGC 7 cut(s) 47, 56, 61, 185, 356, 503, 605
NciI CCSGG 2 cut(s) 19, 20
NdeII GATC 4 cut(s) 244, 499, 580, 672
NlaIII CATG 3 cut(s) 91, 251, 679
NlaIV GGNNCC 2 cut(s) 407, 507
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 689
PfeI GAWTC 4 cut(s) 11, 142, 565, 650
PkrI GCNGC 3 cut(s) 349, 448, 681
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 407, 507
RsaI GTAC 3 cut(s) 35, 407, 667
RsaNI GTAC 3 cut(s) 34, 406, 666
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 3 cut(s) 348, 447, 680
Sau3AI GATC 4 cut(s) 244, 499, 580, 672
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 19, 20
SduI GDGCHC 2 cut(s) 294, 458
SmaI CCCGGG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 1 cut(s) 416
SspMI CTAG 2 cut(s) 180, 632
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 17, 18
TaaI ACNGT 4 cut(s) 109, 130, 242, 665
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 254
TaqII GACCGA 1 cut(s) 131
TasI AATT 1 cut(s) 416
TatI WGTACW 2 cut(s) 33, 665
TfiI GAWTC 4 cut(s) 11, 142, 565, 650
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 3 cut(s) 347, 446, 679
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 3 cut(s) 76, 401, 792
TspGWI ACGGA 1 cut(s) 677
TspMI CCCGGG 1 cut(s) 18
TspRI CASTG 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 18
XspI CTAG 2 cut(s) 180, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.