RLG00000021368

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009784
Physical Location & Seq
Forward (+)
74612972 .. 74615895
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021368

Sequence Viewer

Length: 996 bp
ATGGCGTTGACGGTCAACGTCCAGCGTGGTCTGCTCCAAAATAAGCTCCCACGGAATCTCCGCCACCCACCACGTGTCCCGTTCCGATTGGACCTACCTCCACTCCTCTCCTCGTTTAAAGCCCCCACCCCGATTCCCATCTTCCATTTTACGAGCCTAGATCTTCTTCCTCTTCGTTGTCGACCGAGACAGTACACTGCAAAGATGTCGCCAACTGATTCTACACGCGAGGAGAATGTTTACATGGCCAAGTTGGCTGAGCAAGCTGAGCGTTATGAGGAGATGGTTGAGTTTATGGAGAAGGTCGCAAAGACTGTTGATGTTGAGGAGCTGACTGTGGAGGAAAGAAACCTCCTCTCTGTTGCTTATAAGAATGTGATTGGAGCTAGGAGAGCTTCGTGGAGAATTATCTCTTCCATTGAGCAGAAGGAGGAGAGCAGGGGTAATGAAGATCATGTGTCAATCATCAAGGAGTACAGGAGCAAGATCGAGACCGAACTGAGCAAGATCTGTGATGGGATCTTGAACCTTCTCGAGTCCCACCTTATCCCCTCAGCATCATCTGCTGAGTCCAAGGTGTTCTACCTCAAGATGAAAGGTGACTACCATAGGTACCTTGCTGAATTTAAGACTGGAGGTGAAAGAAAGGAAGCCGCTGAGAGCACATTGTTGGCTTACAAATCTGCTCAGGACATTGCTCTGGCTGAGTTGGCTCCAACTCACCCAATAAGGCTGGGACTTGCTCTCAACTTCTCAGTGTTCTACTATGAAATCCTTAACTCACCAGACCGTGCTTGCAATCTGGCTAAACAGGCTTTCGATGAGGCTATTTCTGAACTTGACACACTGGGGGAAGAATCATACAAGGATAGTACTTTGATCATGCAACTTCTCCGAGACAATCTGACACTGTGGACCTCTGACATCACGGATGATGCTGGGGATGAAATCAAGGAAGCATCAAAGCGTGAATCAGGCGAAGCCCCCGCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000166 GO:0001505 GO:0001932 GO:0001933 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004620 GO:0004623 GO:0004857 GO:0004860 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006469 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006587 GO:0006588 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008144 GO:0008150 GO:0008152 GO:0008426 GO:0009058 GO:0009072 GO:0009308 GO:0009506 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010563 GO:0010605 GO:0012505 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0017076 GO:0018130 GO:0018958 GO:0019207 GO:0019210 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0019887 GO:0030054 GO:0030234 GO:0030291 GO:0030312 GO:0030554 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031570 GO:0032268 GO:0032269 GO:0032553 GO:0032555 GO:0032559 GO:0032768 GO:0032770 GO:0033554 GO:0033673 GO:0034641 GO:0035639 GO:0036094 GO:0042133 GO:0042136 GO:0042221 GO:0042325 GO:0042326 GO:0042427 GO:0042428 GO:0042430 GO:0042435 GO:0043085 GO:0043086 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043549 GO:0044092 GO:0044093 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045786 GO:0045859 GO:0045936 GO:0046189 GO:0046483 GO:0046686 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051341 GO:0051348 GO:0051353 GO:0051716 GO:0051726 GO:0052689 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0097159 GO:0097164 GO:0097367 GO:0098772 GO:1901160 GO:1901162 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901615 GO:1901617
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04142 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04142 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

332

Amino Acids

37.56

Weight (kDa)

5.4

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 82 - 306 8.3e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009784)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02520 AT3G02520 AT5G16050 AT5G16050 AT5G38480 AT5G38480 AT5G38480
fragaria_vesca FvH4_6g45301
malus_domestica MD07G1096000.v1.1 MD17G1074900.v1.1 MD17G1105100.v1.1
prunus_persica Prupe.3G239000_v2.0.a1
pyrus_communis pycom09g01110
rosa_chinensis RchiOBHm_Chr2g0163061
rosa_laevigata RLG00000021368
rosa_multiflora Rmu_sc0003984.1_g000004
rosa_roxburghii Rroxscaffold_2G00087580
rosa_rugosa Rorug02G0500100
rosa_samantha Rh2AG566400 Rh2BG578800
rosa_wichuraiana Rw2G046890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc65I GGTACC 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 181
AccII CGCG 1 cut(s) 228
AciI CCGC 3 cut(s) 61, 654, 987
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 623
AcvI CACGTG 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 74
AfaI GTAC 4 cut(s) 194, 476, 614, 874
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 1 cut(s) 526
AluBI AGCT 5 cut(s) 46, 266, 331, 386, 395
AluI AGCT 5 cut(s) 46, 266, 331, 386, 395
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 3 cut(s) 181, 485, 891
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 623
Asp718I GGTACC 1 cut(s) 612
AspS9I GGNCC 2 cut(s) 91, 915
AsuHPI GGTGA 4 cut(s) 611, 650, 713, 774
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 91, 915
BalI TGGCCA 1 cut(s) 248
BanI GGYRCC 1 cut(s) 612
BbrPI CACGTG 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 665
BbvCI CCTCAGC 1 cut(s) 553
BccI CCATC 3 cut(s) 146, 277, 509
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 3 cut(s) 181, 485, 891
BfaI CTAG 2 cut(s) 158, 387
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 2 cut(s) 160, 507
BisI GCNGC 1 cut(s) 654
BlpI GCTNAGC 2 cut(s) 258, 267
BlsI GCNGC 1 cut(s) 655
BmcAI AGTACT 1 cut(s) 874
Bme18I GGWCC 2 cut(s) 91, 915
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 91, 915
BmiI GGNNCC 2 cut(s) 614, 714
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 3 cut(s) 566, 925, 968
BmuI ACTGGG 1 cut(s) 857
BpmI CTGGAG 1 cut(s) 654
Bpu10I CCTNAGC 2 cut(s) 553, 687
Bpu1102I GCTNAGC 2 cut(s) 258, 267
BpuEI CTTGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 2 cut(s) 50, 573
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 87, 117
Bse1I ACTGG 2 cut(s) 637, 852
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 50, 573
BseGI GGATG 2 cut(s) 937, 949
BseJI GATNNNNATC 1 cut(s) 137
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 9 cut(s) 249, 258, 491, 558, 567, 648, 696, 701, 768
BseNI ACTGG 2 cut(s) 637, 852
BseRI GAGGAG 7 cut(s) 95, 100, 245, 293, 341, 344, 446
BseYI CCCAGC 2 cut(s) 733, 938
Bsh1236I CGCG 1 cut(s) 228
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 1 cut(s) 248
BshNI GGYRCC 1 cut(s) 612
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 533
BslFI GGGAC 3 cut(s) 62, 523, 750
BsmAI GTCTC 3 cut(s) 181, 485, 891
BsmFI GGGAC 3 cut(s) 62, 523, 750
BsnI GGCC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 6 cut(s) 160, 451, 486, 507, 519, 879
Bsp1720I GCTNAGC 2 cut(s) 258, 267
BspACI CCGC 3 cut(s) 61, 654, 987
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 9 cut(s) 250, 259, 492, 559, 566, 649, 697, 700, 767
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 614, 714
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 612
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 693
BsrI ACTGG 2 cut(s) 637, 852
BssECI CCNNGG 2 cut(s) 50, 573
BssMI GATC 6 cut(s) 160, 451, 486, 507, 519, 879
BssT1I CCWWGG 1 cut(s) 573
Bst4CI ACNGT 7 cut(s) 13, 192, 316, 337, 791, 912, 993
Bst6I CTCTTC 2 cut(s) 177, 418
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 264, 796
BstDEI CTNAG 9 cut(s) 258, 267, 500, 553, 567, 657, 687, 705, 754
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 2 cut(s) 937, 949
BstFNI CGCG 1 cut(s) 228
BstKTI GATC 6 cut(s) 163, 454, 489, 510, 522, 882
BstMAI GTCTC 3 cut(s) 181, 485, 891
BstMBI GATC 6 cut(s) 160, 451, 486, 507, 519, 879
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 8 cut(s) 31, 254, 263, 268, 392, 563, 710, 812
BstUI CGCG 1 cut(s) 228
BstX2I RGATCY 3 cut(s) 160, 507, 519
BstYI RGATCY 3 cut(s) 160, 507, 519
BsuRI GGCC 1 cut(s) 248
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 2 cut(s) 937, 949
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 4 cut(s) 195, 762, 845, 908
Cac8I GCNNGC 2 cut(s) 264, 796
Cfr13I GGNCC 2 cut(s) 91, 915
Csp6I GTAC 4 cut(s) 193, 475, 613, 873
CviAII CATG 3 cut(s) 244, 455, 883
CviQI GTAC 4 cut(s) 193, 475, 613, 873
DdeI CTNAG 9 cut(s) 258, 267, 500, 553, 567, 657, 687, 705, 754
DpnI GATC 6 cut(s) 162, 453, 488, 509, 521, 881
DpnII GATC 6 cut(s) 160, 451, 486, 507, 519, 879
DraI TTTAAA 1 cut(s) 118
DraIII CACNNNGTG 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 177, 418
EarI CTCTTC 2 cut(s) 177, 418
EciI GGCGGA 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 573
Eco31I GGTCTC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 91, 915
Eco72I CACGTG 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 3 cut(s) 247, 458, 886
FaiI YATR 9 cut(s) 245, 276, 296, 369, 456, 609, 768, 862, 884
FaqI GGGAC 3 cut(s) 62, 523, 750
FatI CATG 3 cut(s) 243, 454, 882
FauI CCCGC 1 cut(s) 994
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 654
FokI GGATG 2 cut(s) 944, 956
Fsp4HI GCNGC 1 cut(s) 654
FspBI CTAG 2 cut(s) 158, 387
GluI GCNGC 1 cut(s) 654
GsaI CCCAGC 2 cut(s) 737, 942
GsuI CTGGAG 1 cut(s) 654
HaeIII GGCC 1 cut(s) 248
Hin1II CATG 3 cut(s) 247, 458, 886
HincII GTYRAC 3 cut(s) 9, 16, 182
HindII GTYRAC 3 cut(s) 9, 16, 182
HinfI GANTC 7 cut(s) 55, 133, 218, 536, 569, 857, 971
HphI GGTGA 4 cut(s) 611, 650, 713, 774
Hpy166II GTNNAC 6 cut(s) 9, 16, 182, 195, 241, 915
Hpy188I TCNGA 5 cut(s) 86, 835, 896, 906, 922
Hpy188III TCNNGA 5 cut(s) 490, 523, 533, 589, 689
Hpy8I GTNNAC 6 cut(s) 9, 16, 182, 195, 241, 915
HpyAV CCTTC 3 cut(s) 295, 421, 539
HpyCH4III ACNGT 7 cut(s) 13, 192, 316, 337, 791, 912, 993
HpyCH4IV ACGT 2 cut(s) 18, 73
HpyCH4V TGCA 3 cut(s) 200, 798, 886
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 254, 263, 268, 392, 563, 710, 812
HpyF3I CTNAG 9 cut(s) 258, 267, 500, 553, 567, 657, 687, 705, 754
HpySE526I ACGT 2 cut(s) 18, 73
Hsp92II CATG 3 cut(s) 247, 458, 886
KpnI GGTACC 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 6 cut(s) 160, 451, 486, 507, 519, 879
LmnI GCTCC 6 cut(s) 39, 51, 328, 383, 480, 718
LweI GCATC 3 cut(s) 566, 925, 968
MaeI CTAG 2 cut(s) 158, 387
MaeII ACGT 2 cut(s) 18, 73
MaeIII GTNAC 1 cut(s) 599
MalI GATC 6 cut(s) 162, 453, 488, 509, 521, 881
MboI GATC 6 cut(s) 160, 451, 486, 507, 519, 879
MboII GAAGA 7 cut(s) 133, 155, 158, 164, 405, 461, 866
MflI RGATCY 3 cut(s) 160, 507, 519
MhlI GDGCHC 1 cut(s) 665
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 2 cut(s) 405, 623
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 2 cut(s) 545, 578
MmeI TCCRAC 1 cut(s) 740
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
MseI TTAA 3 cut(s) 117, 627, 777
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 656
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 8 cut(s) 31, 254, 263, 268, 392, 563, 710, 812
NdeII GATC 6 cut(s) 160, 451, 486, 507, 519, 879
NlaIII CATG 3 cut(s) 247, 458, 886
NlaIV GGNNCC 2 cut(s) 614, 714
NmuCI GTSAC 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 533
PfeI GAWTC 5 cut(s) 55, 133, 218, 857, 971
PkrI GCNGC 1 cut(s) 655
PleI GAGTC 2 cut(s) 544, 577
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
PpsI GAGTC 2 cut(s) 544, 577
Ppu21I YACGTR 1 cut(s) 74
PsiI TTATAA 1 cut(s) 369
PspCI CACGTG 1 cut(s) 74
PspFI CCCAGC 2 cut(s) 733, 938
PspN4I GGNNCC 2 cut(s) 614, 714
PspPI GGNCC 2 cut(s) 91, 915
PsuI RGATCY 3 cut(s) 160, 507, 519
RsaI GTAC 4 cut(s) 194, 476, 614, 874
RsaNI GTAC 4 cut(s) 193, 475, 613, 873
SalI GTCGAC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 117, 627, 777
SatI GCNGC 1 cut(s) 654
Sau3AI GATC 6 cut(s) 160, 451, 486, 507, 519, 879
Sau96I GGNCC 2 cut(s) 91, 915
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 2 cut(s) 545, 578
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 3 cut(s) 566, 925, 968
Sfr274I CTCGAG 1 cut(s) 533
SinI GGWCC 2 cut(s) 91, 915
SlaI CTCGAG 1 cut(s) 533
SmlI CTYRAG 2 cut(s) 533, 587
SmoI CTYRAG 2 cut(s) 533, 587
Sse9I AATT 2 cut(s) 405, 623
SsiI CCGC 3 cut(s) 61, 654, 987
SspMI CTAG 2 cut(s) 158, 387
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 7 cut(s) 13, 192, 316, 337, 791, 912, 993
TaiI ACGT 2 cut(s) 21, 76
TaqI TCGA 4 cut(s) 181, 489, 534, 819
TaqII GACCGA 2 cut(s) 199, 509
TasI AATT 2 cut(s) 405, 623
TatI WGTACW 3 cut(s) 192, 474, 872
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 5 cut(s) 55, 133, 218, 857, 971
Tru1I TTAA 3 cut(s) 117, 627, 777
Tru9I TTAA 3 cut(s) 117, 627, 777
TscAI CASTG 4 cut(s) 202, 762, 852, 915
TseFI GTSAC 1 cut(s) 599
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 4 cut(s) 462, 608, 783, 960
TspGWI ACGGA 2 cut(s) 67, 944
TspRI CASTG 4 cut(s) 202, 762, 852, 915
VpaK11BI GGWCC 2 cut(s) 91, 915
XapI RAATTY 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 181
XspI CTAG 2 cut(s) 158, 387
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.