RchiOBHm_Chr2g0163061

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009784
Physical Location & Seq
Forward (+)
78710780 .. 78713828
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53126

Sequence Viewer

Length: 792 bp
ATGTCGCCAACTGATTCTACACGCGAGGAGAATGTTTACATGGCCAAGTTGGCTGAGCAAGCTGAGCGTTATGAGGAGATGGTTGAGTTTATGGAGAAGGTCGCAAAGACTGTTGATGTTGAGGAGCTAACTGTGGAGGAAAGAAACCTCCTCTCCGTTGCTTATAAGAATGTGATTGGAGCTAGGAGAGCTTCGTGGAGAATTATCTCTTCCATTGAGCAGAAGGAGGAGAGCAGGGGTAATGAAGATCATGTGTCAATCATCAAGGAGTACAGGAGCAAGATCGAGACCGAACTGAGCAAGATCTGTGATGGGATCTTGAACCTTCTCGAGTCCCACCTTATCCCCTCAGCATCATCTGCTGAGTCCAAGGTGTTCTACCTCAAGATGAAAGGTGACTACCATAGGTACCTTGCTGAATTTAAGACTGGAGGTGAAAGAAAAGAAGCAGCTGAGAGCACATTGTTGGCTTACAAATCTGCTCAGGACATTGCTCTGGCTGAGTTGGCTCCAACTCACCCAATAAGGCTGGGACTTGCTCTCAACTTCTCAGTGTTCTACTATGAAATCCTTAACTCACCAGACCGTGCTTGCAATCTGGCTAAACAGGCTTTCGATGAGGCTATTTCTGAACTTGACACACTGGGTGAAGAATCATACAAGGACAGTACTTTGATCATGCAACTCCTCCGAGACAATCTGACACTGTGGACCTCTGACATCACGGATGATGCTGGGGATGAAATCAAGGAAGCATCAAAGCGTGAATCAGGCGAAGCCCCCGCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000166 GO:0001505 GO:0001932 GO:0001933 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004620 GO:0004623 GO:0004857 GO:0004860 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006469 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006587 GO:0006588 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008144 GO:0008150 GO:0008152 GO:0008426 GO:0009058 GO:0009072 GO:0009308 GO:0009506 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010563 GO:0010605 GO:0012505 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0017076 GO:0018130 GO:0018958 GO:0019207 GO:0019210 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0019887 GO:0030054 GO:0030234 GO:0030291 GO:0030312 GO:0030554 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031570 GO:0032268 GO:0032269 GO:0032553 GO:0032555 GO:0032559 GO:0032768 GO:0032770 GO:0033554 GO:0033673 GO:0034641 GO:0035639 GO:0036094 GO:0042133 GO:0042136 GO:0042221 GO:0042325 GO:0042326 GO:0042427 GO:0042428 GO:0042430 GO:0042435 GO:0043085 GO:0043086 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043549 GO:0044092 GO:0044093 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045786 GO:0045859 GO:0045936 GO:0046189 GO:0046483 GO:0046686 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051341 GO:0051348 GO:0051353 GO:0051716 GO:0051726 GO:0052689 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0097159 GO:0097164 GO:0097367 GO:0098772 GO:1901160 GO:1901162 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901615 GO:1901617
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04142 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04142 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

263

Amino Acids

29.71

Weight (kDa)

4.75

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 238 3.9e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009784)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02520 AT3G02520 AT5G16050 AT5G16050 AT5G38480 AT5G38480 AT5G38480
fragaria_vesca FvH4_6g45301
malus_domestica MD07G1096000.v1.1 MD17G1074900.v1.1 MD17G1105100.v1.1
prunus_persica Prupe.3G239000_v2.0.a1
pyrus_communis pycom09g01110
rosa_chinensis RchiOBHm_Chr2g0163061
rosa_laevigata RLG00000021368
rosa_multiflora Rmu_sc0003984.1_g000004
rosa_roxburghii Rroxscaffold_2G00087580
rosa_rugosa Rorug02G0500100
rosa_samantha Rh2AG566400 Rh2BG578800
rosa_wichuraiana Rw2G046890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 408
AccII CGCG 1 cut(s) 24
AciI CCGC 1 cut(s) 783
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 419
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 3 cut(s) 272, 410, 670
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 5 cut(s) 62, 127, 182, 191, 452
AluI AGCT 5 cut(s) 62, 127, 182, 191, 452
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 2 cut(s) 281, 687
AlwI GGATC 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 419
Asp718I GGTACC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 711
AsuHPI GGTGA 5 cut(s) 407, 446, 509, 570, 659
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 711
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 461
BbvCI CCTCAGC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 461
BccI CCATC 2 cut(s) 73, 305
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 2 cut(s) 281, 687
BfaI CTAG 1 cut(s) 183
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 1 cut(s) 303
BisI GCNGC 1 cut(s) 450
BlpI GCTNAGC 2 cut(s) 54, 63
BlsI GCNGC 1 cut(s) 451
BmcAI AGTACT 1 cut(s) 670
Bme18I GGWCC 1 cut(s) 711
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 711
BmiI GGNNCC 2 cut(s) 410, 510
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 3 cut(s) 362, 721, 764
BmuI ACTGGG 1 cut(s) 653
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 2 cut(s) 349, 483
Bpu1102I GCTNAGC 2 cut(s) 54, 63
BpuEI CTTGAG 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bse1I ACTGG 2 cut(s) 433, 648
Bse3DI GCAATG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 733, 745
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 9 cut(s) 45, 54, 287, 354, 363, 444, 492, 497, 564
BseNI ACTGG 2 cut(s) 433, 648
BseRI GAGGAG 6 cut(s) 41, 89, 137, 140, 242, 677
BseXI GCAGC 1 cut(s) 461
BseYI CCCAGC 2 cut(s) 529, 734
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 1 cut(s) 44
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 329
BslFI GGGAC 2 cut(s) 319, 546
BsmAI GTCTC 2 cut(s) 281, 687
BsmFI GGGAC 2 cut(s) 319, 546
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 5 cut(s) 247, 282, 303, 315, 675
Bsp1720I GCTNAGC 2 cut(s) 54, 63
BspACI CCGC 1 cut(s) 783
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 9 cut(s) 46, 55, 288, 355, 362, 445, 493, 496, 563
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 410, 510
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 489
BsrI ACTGG 2 cut(s) 433, 648
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 5 cut(s) 247, 282, 303, 315, 675
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 6 cut(s) 112, 133, 587, 668, 708, 789
Bst6I CTCTTC 1 cut(s) 214
BstAPI GCANNNNNTGC 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 60, 592
BstDEI CTNAG 9 cut(s) 54, 63, 296, 349, 363, 453, 483, 501, 550
BstF5I GGATG 2 cut(s) 733, 745
BstFNI CGCG 1 cut(s) 24
BstKTI GATC 5 cut(s) 250, 285, 306, 318, 678
BstMAI GTCTC 2 cut(s) 281, 687
BstMBI GATC 5 cut(s) 247, 282, 303, 315, 675
BstMWI GCNNNNNNNGC 7 cut(s) 50, 59, 64, 188, 359, 506, 608
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 461
BstX2I RGATCY 2 cut(s) 303, 315
BstYI RGATCY 2 cut(s) 303, 315
BsuRI GGCC 1 cut(s) 44
BtsCI GGATG 2 cut(s) 733, 745
BtsIMutI CAGTG 3 cut(s) 558, 641, 704
Cac8I GCNNGC 2 cut(s) 60, 592
Cfr13I GGNCC 1 cut(s) 711
Csp6I GTAC 3 cut(s) 271, 409, 669
CviAII CATG 3 cut(s) 40, 251, 679
CviQI GTAC 3 cut(s) 271, 409, 669
DdeI CTNAG 9 cut(s) 54, 63, 296, 349, 363, 453, 483, 501, 550
DpnI GATC 5 cut(s) 249, 284, 305, 317, 677
DpnII GATC 5 cut(s) 247, 282, 303, 315, 675
DraIII CACNNNGTG 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 3 cut(s) 43, 254, 682
FaiI YATR 9 cut(s) 41, 72, 92, 165, 252, 405, 564, 658, 680
FaqI GGGAC 2 cut(s) 319, 546
FatI CATG 3 cut(s) 39, 250, 678
FauI CCCGC 1 cut(s) 790
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 2 cut(s) 740, 752
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 1 cut(s) 183
GluI GCNGC 1 cut(s) 450
GsaI CCCAGC 2 cut(s) 533, 738
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 44
Hin1II CATG 3 cut(s) 43, 254, 682
HinfI GANTC 5 cut(s) 14, 332, 365, 653, 767
HphI GGTGA 5 cut(s) 407, 446, 509, 570, 659
Hpy166II GTNNAC 2 cut(s) 37, 711
Hpy188I TCNGA 4 cut(s) 631, 692, 702, 718
Hpy188III TCNNGA 5 cut(s) 286, 319, 329, 385, 485
Hpy8I GTNNAC 2 cut(s) 37, 711
HpyAV CCTTC 3 cut(s) 91, 217, 335
HpyCH4III ACNGT 6 cut(s) 112, 133, 587, 668, 708, 789
HpyCH4V TGCA 2 cut(s) 594, 682
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 59, 64, 188, 359, 506, 608
HpyF3I CTNAG 9 cut(s) 54, 63, 296, 349, 363, 453, 483, 501, 550
Hsp92II CATG 3 cut(s) 43, 254, 682
KpnI GGTACC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 5 cut(s) 247, 282, 303, 315, 675
LmnI GCTCC 4 cut(s) 124, 179, 276, 514
Lsp1109I GCAGC 1 cut(s) 461
LweI GCATC 3 cut(s) 362, 721, 764
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 395
MalI GATC 5 cut(s) 249, 284, 305, 317, 677
MboI GATC 5 cut(s) 247, 282, 303, 315, 675
MboII GAAGA 3 cut(s) 201, 257, 662
MflI RGATCY 2 cut(s) 303, 315
MhlI GDGCHC 1 cut(s) 461
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 201, 419
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 341, 374
MmeI TCCRAC 1 cut(s) 536
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 423, 573
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 452
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 7 cut(s) 50, 59, 64, 188, 359, 506, 608
NdeII GATC 5 cut(s) 247, 282, 303, 315, 675
NlaIII CATG 3 cut(s) 43, 254, 682
NlaIV GGNNCC 2 cut(s) 410, 510
NmuCI GTSAC 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 329
PfeI GAWTC 3 cut(s) 14, 653, 767
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 2 cut(s) 340, 373
PpsI GAGTC 2 cut(s) 340, 373
PsiI TTATAA 1 cut(s) 165
PspFI CCCAGC 2 cut(s) 529, 734
PspN4I GGNNCC 2 cut(s) 410, 510
PspPI GGNCC 1 cut(s) 711
PsuI RGATCY 2 cut(s) 303, 315
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 272, 410, 670
RsaNI GTAC 3 cut(s) 271, 409, 669
SaqAI TTAA 2 cut(s) 423, 573
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 5 cut(s) 247, 282, 303, 315, 675
Sau96I GGNCC 1 cut(s) 711
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 2 cut(s) 341, 374
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 3 cut(s) 362, 721, 764
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 711
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 329, 383
SmoI CTYRAG 2 cut(s) 329, 383
Sse9I AATT 2 cut(s) 201, 419
SsiI CCGC 1 cut(s) 783
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 6 cut(s) 112, 133, 587, 668, 708, 789
TaqI TCGA 3 cut(s) 285, 330, 615
TaqII GACCGA 1 cut(s) 305
TasI AATT 2 cut(s) 201, 419
TatI WGTACW 2 cut(s) 270, 668
TfiI GAWTC 3 cut(s) 14, 653, 767
Tru1I TTAA 2 cut(s) 423, 573
Tru9I TTAA 2 cut(s) 423, 573
TscAI CASTG 3 cut(s) 558, 648, 711
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 4 cut(s) 258, 404, 579, 756
TspGWI ACGGA 2 cut(s) 145, 740
TspRI CASTG 3 cut(s) 558, 648, 711
VpaK11BI GGWCC 1 cut(s) 711
XapI RAATTY 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 1 cut(s) 183
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.