MD17G1105100.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009784
Physical Location & Seq
Forward (+)
8914153 .. 8917345
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1105100.v1.1.491

Sequence Viewer

Length: 909 bp
ATGTCGCCACCTGATTCTTCACGTGAGGAGAATGTGTACATGGCCAAGTTGGCCGAACAGGCTGAGAGATATGAGGAGATGGTTGAGTTTATGGAGAAAGTTGCCAAGACTGTTGATGTTGAGGAGCTAACAGTGGAGGAAAGAAACCTCCTCTCCGTGGCTTATAAGAATGTCATTGGAGCTAGGAGAGCTTCATGGAGGATCATATCTTCCATTGAGCAGAAGGAGGAGAGCAGAGGGAATGAGGATCATGTTGCGATTATCAAGGAATACAGGAGCAAGATCGAGGCTGAGCTCAGCAAGATCTGTGATGGGATATTGAACCTTCTTGAGTCGCACCTCATTCCGTCTGCCTCTTCCGCCGAGTCCAAGGTGTTTTACCTCAAGATGAAAGGTGACTACCACAGGTACCTTGCAGAGTTTAAGACTGGAGGCGAGAGAAAGGAAGCAGCTGAGAGCACTTTGTTGGCTTACAAGTCTGCTCAGGACATTGCCCTCGCCGAGTTGGCTCCAACCCACCCAATAAGGCTTGGACTTGCACTCAACTTCTCAGTCTTCTACTATGAAATCCTTAACTCACCTGATCGTGCTTGCAATCTGGCAAAACAGGCTTTTGATGAGGCTATTTCTGAGCTTGACACATTGGGTGAGGAATCCTACAAGGATAGCACTTTGATCATGCAGCTTCTCCGAGACAATCTTACACTCTGGACTTCTGATATCACGGATCTCTCATCCTCTTTCCCTCGTTATGCATGTACACATATGACCGAAAAGGGGGTCTTGACTCTTGAGATTGGGATGCATCATTATAGTGATACCGATTATGCAACCTATCATTGCAATTCAGATGATGCCGGGGACGAAATCAAGGAAGCATCAAAGCGCGAATCCAGTGAAGCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000166 GO:0001505 GO:0001932 GO:0001933 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004620 GO:0004623 GO:0004857 GO:0004860 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006469 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006587 GO:0006588 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008144 GO:0008150 GO:0008152 GO:0008426 GO:0009058 GO:0009072 GO:0009308 GO:0009506 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010563 GO:0010605 GO:0012505 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0017076 GO:0018130 GO:0018958 GO:0019207 GO:0019210 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0019887 GO:0030054 GO:0030234 GO:0030291 GO:0030312 GO:0030554 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031570 GO:0032268 GO:0032269 GO:0032553 GO:0032555 GO:0032559 GO:0032768 GO:0032770 GO:0033554 GO:0033673 GO:0034641 GO:0035639 GO:0036094 GO:0042133 GO:0042136 GO:0042221 GO:0042325 GO:0042326 GO:0042427 GO:0042428 GO:0042430 GO:0042435 GO:0043085 GO:0043086 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043549 GO:0044092 GO:0044093 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045786 GO:0045859 GO:0045936 GO:0046189 GO:0046483 GO:0046686 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051341 GO:0051348 GO:0051353 GO:0051716 GO:0051726 GO:0052689 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0097159 GO:0097164 GO:0097367 GO:0098772 GO:1901160 GO:1901162 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901615 GO:1901617
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04142 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04142 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

303

Amino Acids

34.17

Weight (kDa)

4.83

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 238 7.6e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009784)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02520 AT3G02520 AT5G16050 AT5G16050 AT5G38480 AT5G38480 AT5G38480
fragaria_vesca FvH4_6g45301
malus_domestica MD07G1096000.v1.1 MD17G1074900.v1.1 MD17G1105100.v1.1
prunus_persica Prupe.3G239000_v2.0.a1
pyrus_communis pycom09g01110
rosa_chinensis RchiOBHm_Chr2g0163061
rosa_laevigata RLG00000021368
rosa_multiflora Rmu_sc0003984.1_g000004
rosa_roxburghii Rroxscaffold_2G00087580
rosa_rugosa Rorug02G0500100
rosa_samantha Rh2AG566400 Rh2BG578800
rosa_wichuraiana Rw2G046890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 408
AccII CGCG 1 cut(s) 888
AciI CCGC 1 cut(s) 360
AclWI GGATC 3 cut(s) 209, 255, 735
AcoI YGGCCR 2 cut(s) 42, 51
AcvI CACGTG 1 cut(s) 23
AfaI GTAC 3 cut(s) 38, 410, 760
AfiI CCNNNNNNNGG 2 cut(s) 157, 777
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 7 cut(s) 127, 182, 191, 295, 452, 634, 685
AluI AGCT 7 cut(s) 127, 182, 191, 295, 452, 634, 685
Alw21I GWGCWC 2 cut(s) 297, 461
Alw26I GTCTC 1 cut(s) 687
AlwI GGATC 3 cut(s) 209, 255, 735
AoxI GGCC 2 cut(s) 42, 51
ApeKI GCWGC 2 cut(s) 449, 682
ArsI GACNNNNNNTTYG 2 cut(s) 765, 797
Asp718I GGTACC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 888
AsuC2I CCSGG 1 cut(s) 859
AsuHPI GGTGA 3 cut(s) 407, 570, 659
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 297
BbrPI CACGTG 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 547
Bbv12I GWGCWC 2 cut(s) 297, 461
BbvI GCAGC 2 cut(s) 461, 694
BccI CCATC 2 cut(s) 73, 305
BclI TGATCA 1 cut(s) 675
BcnI CCSGG 1 cut(s) 859
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 1 cut(s) 183
BglI GCCNNNNNGGC 3 cut(s) 50, 59, 506
BglII AGATCT 1 cut(s) 303
BisI GCNGC 2 cut(s) 450, 683
BlpI GCTNAGC 2 cut(s) 291, 296
BlsI GCNGC 2 cut(s) 451, 684
Bme1390I CCNGG 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 410, 510
BmrFI CCNGG 1 cut(s) 859
BmsI GCATC 4 cut(s) 792, 814, 844, 887
BpiI GAAGAC 1 cut(s) 547
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 483
Bpu1102I GCTNAGC 2 cut(s) 291, 296
BpuEI CTTGAG 3 cut(s) 350, 368, 812
BpuMI CCSGG 1 cut(s) 859
BsaAI YACGTR 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 156, 369, 858
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 137, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 777
Bse1I ACTGG 2 cut(s) 433, 894
Bse3DI GCAATG 2 cut(s) 489, 838
BseDI CCNNGG 3 cut(s) 156, 369, 858
BseGI GGATG 2 cut(s) 734, 807
BseLI CCNNNNNNNGG 2 cut(s) 157, 777
BseMI GCAATG 2 cut(s) 489, 838
BseMII CTCAG 7 cut(s) 54, 282, 310, 444, 497, 564, 621
BseNI ACTGG 2 cut(s) 433, 894
BseRI GAGGAG 5 cut(s) 41, 89, 137, 140, 242
BseXI GCAGC 2 cut(s) 461, 694
Bsh1236I CGCG 1 cut(s) 888
BshFI GGCC 2 cut(s) 44, 53
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 297, 461
BsiSI CCGG 1 cut(s) 858
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 2 cut(s) 157, 777
BsmAI GTCTC 1 cut(s) 687
BsmFI GGGAC 1 cut(s) 875
BsnI GGCC 2 cut(s) 44, 53
Bsp1286I GDGCHC 2 cut(s) 297, 461
Bsp1407I TGTACA 2 cut(s) 36, 758
Bsp143I GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
Bsp1720I GCTNAGC 2 cut(s) 291, 296
BspACI CCGC 1 cut(s) 360
BspANI GGCC 2 cut(s) 44, 53
BspCNI CTCAG 7 cut(s) 55, 283, 309, 445, 496, 563, 622
BspFNI CGCG 1 cut(s) 888
BspLI GGNNCC 2 cut(s) 410, 510
BspPI GGATC 3 cut(s) 209, 255, 735
BspT107I GGYRCC 1 cut(s) 408
BsrDI GCAATG 2 cut(s) 489, 838
BsrGI TGTACA 2 cut(s) 36, 758
BsrI ACTGG 2 cut(s) 433, 894
BssECI CCNNGG 3 cut(s) 156, 369, 858
BssMI GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 112, 133, 906
Bst6I CTCTTC 1 cut(s) 361
BstAUI TGTACA 2 cut(s) 36, 758
BstBAI YACGTR 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 7 cut(s) 63, 291, 296, 453, 483, 550, 630
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 734, 807
BstFNI CGCG 1 cut(s) 888
BstHHI GCGC 1 cut(s) 888
BstKTI GATC 7 cut(s) 204, 250, 285, 306, 586, 678, 730
BstMAI GTCTC 1 cut(s) 687
BstMBI GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
BstMWI GCNNNNNNNGC 6 cut(s) 50, 59, 188, 359, 506, 608
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 1 cut(s) 857
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 2 cut(s) 461, 694
BstV2I GAAGAC 1 cut(s) 547
BstX2I RGATCY 2 cut(s) 303, 727
BstYI RGATCY 2 cut(s) 303, 727
BsuRI GGCC 2 cut(s) 44, 53
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 734, 807
BtsIMutI CAGTG 2 cut(s) 138, 901
Cac8I GCNNGC 1 cut(s) 592
CfoI GCGC 1 cut(s) 888
Csp6I GTAC 3 cut(s) 37, 409, 759
CviAII CATG 5 cut(s) 40, 195, 251, 679, 756
CviQI GTAC 3 cut(s) 37, 409, 759
DdeI CTNAG 7 cut(s) 63, 291, 296, 453, 483, 550, 630
DpnI GATC 7 cut(s) 203, 249, 284, 305, 585, 677, 729
DpnII GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
EaeI YGGCCR 2 cut(s) 42, 51
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 721
Eco53kI GAGCTC 1 cut(s) 295
Eco72I CACGTG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 295
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 2 cut(s) 757, 807
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 43, 198, 254, 682, 759
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FaqI GGGAC 1 cut(s) 875
FatI CATG 5 cut(s) 39, 194, 250, 678, 755
FauNDI CATATG 1 cut(s) 765
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 2 cut(s) 450, 683
FokI GGATG 2 cut(s) 721, 814
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 450, 683
FspBI CTAG 1 cut(s) 183
GlaI GCGC 1 cut(s) 887
GluI GCNGC 2 cut(s) 450, 683
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 44, 53
HapII CCGG 1 cut(s) 858
HhaI GCGC 1 cut(s) 888
Hin1II CATG 5 cut(s) 43, 198, 254, 682, 759
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HinfI GANTC 6 cut(s) 14, 332, 365, 653, 787, 890
HpaII CCGG 1 cut(s) 858
HphI GGTGA 3 cut(s) 407, 570, 659
Hpy166II GTNNAC 2 cut(s) 37, 761
Hpy188I TCNGA 4 cut(s) 631, 692, 718, 850
Hpy188III TCNNGA 6 cut(s) 329, 385, 485, 709, 784, 791
Hpy8I GTNNAC 2 cut(s) 37, 761
HpyAV CCTTC 2 cut(s) 217, 335
HpyCH4III ACNGT 3 cut(s) 112, 133, 906
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 8 cut(s) 416, 539, 594, 682, 755, 805, 830, 843
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 59, 188, 359, 506, 608
HpyF3I CTNAG 7 cut(s) 63, 291, 296, 453, 483, 550, 630
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 5 cut(s) 43, 198, 254, 682, 759
HspAI GCGC 1 cut(s) 886
KpnI GGTACC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
LmnI GCTCC 4 cut(s) 124, 179, 276, 514
Lsp1109I GCAGC 2 cut(s) 461, 694
LweI GCATC 4 cut(s) 792, 814, 844, 887
MaeI CTAG 1 cut(s) 183
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 1 cut(s) 395
MalI GATC 7 cut(s) 203, 249, 284, 305, 585, 677, 729
MboI GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
MboII GAAGA 4 cut(s) 9, 201, 348, 547
MflI RGATCY 2 cut(s) 303, 727
MhlI GDGCHC 2 cut(s) 297, 461
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 1 cut(s) 844
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 3 cut(s) 341, 374, 781
MmeI TCCRAC 1 cut(s) 536
Mox20I TGGCCA 1 cut(s) 44
Mph1103I ATGCAT 2 cut(s) 757, 807
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 423, 573
MslI CAYNNNNRTG 1 cut(s) 813
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 858
MspR9I CCNGG 1 cut(s) 859
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 6 cut(s) 50, 59, 188, 359, 506, 608
NciI CCSGG 1 cut(s) 859
NdeI CATATG 1 cut(s) 765
NdeII GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
NlaIII CATG 5 cut(s) 43, 198, 254, 682, 759
NlaIV GGNNCC 2 cut(s) 410, 510
NmeAIII GCCGAG 2 cut(s) 388, 526
NmuCI GTSAC 1 cut(s) 395
NsiI ATGCAT 2 cut(s) 757, 807
NspI RCATGY 1 cut(s) 759
PfeI GAWTC 3 cut(s) 14, 653, 890
PkrI GCNGC 2 cut(s) 451, 684
PleI GAGTC 3 cut(s) 340, 373, 781
PmaCI CACGTG 1 cut(s) 23
PmlI CACGTG 1 cut(s) 23
PpsI GAGTC 3 cut(s) 340, 373, 781
Ppu21I YACGTR 1 cut(s) 23
PsiI TTATAA 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 297
PspCI CACGTG 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 410, 510
PsuI RGATCY 2 cut(s) 303, 727
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 38, 410, 760
RsaNI GTAC 3 cut(s) 37, 409, 759
RseI CAYNNNNRTG 1 cut(s) 813
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 423, 573
SatI GCNGC 2 cut(s) 450, 683
Sau3AI GATC 7 cut(s) 201, 247, 282, 303, 583, 675, 727
SchI GAGTC 3 cut(s) 341, 374, 781
ScrFI CCNGG 1 cut(s) 859
SduI GDGCHC 2 cut(s) 297, 461
SfaNI GCATC 4 cut(s) 792, 814, 844, 887
SfiI GGCCNNNNNGGCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 3 cut(s) 329, 383, 791
SmoI CTYRAG 3 cut(s) 329, 383, 791
Sse9I AATT 1 cut(s) 844
SsiI CCGC 1 cut(s) 360
SspMI CTAG 1 cut(s) 183
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 857
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 112, 133, 906
TaiI ACGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 285
TaqII GACCGA 1 cut(s) 785
TasI AATT 1 cut(s) 844
TatI WGTACW 2 cut(s) 36, 758
TfiI GAWTC 3 cut(s) 14, 653, 890
Tru1I TTAA 2 cut(s) 423, 573
Tru9I TTAA 2 cut(s) 423, 573
TscAI CASTG 2 cut(s) 138, 901
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 2 cut(s) 449, 682
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 3 cut(s) 183, 404, 579
TspGWI ACGGA 3 cut(s) 145, 336, 740
TspRI CASTG 2 cut(s) 138, 901
XceI RCATGY 1 cut(s) 759
XspI CTAG 1 cut(s) 183
Zsp2I ATGCAT 2 cut(s) 757, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.