AT5G16530

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013198
Physical Location & Seq
Forward (+)
5400600 .. 5402845
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16530.1

Sequence Viewer

Length: 1056 bp
ATGATAAATTGTGGAGATGTTTACAAGGTAATCGAAGCAATGGTTCCACTCTATGTAGCCTTAATCTTAGGCTATGGCTCTGTGAAATGGTGGCACATCTTCACGCGTGATCAATGTGATGCTATAAACCGTCTCGTTTGCTATTTCACCCTGCCGCTCTTCACCATTGAGTTCACGGCCCATGTCGACCCGTTCAACATGAATTACCGGTTCATCGCGGCCGATGTCCTCTCTAAGGTCATCATAGTCACGGTCTTAGCGTTGTGGGCCAAATATAGCAACAAAGGAAGCTATTGTTGGTCCATTACTAGTTTCTCTCTATGCACTCTTACTAACTCTCTTGTCGTTGGTGTGCCATTGGCTAAGGCAATGTACGGACAACAGGCTGTTGATCTGGTGGTTCAATCCTCGGTATTTCAAGCCATCGTGTGGCTCACACTCTTGTTGTTTGTCTTAGAGTTTAGAAAAGCCGGATTTAGTAGTAATAATATCAGCGACGTACAAGTAGACAACATCAATATCGAAAGTGGAAAAAGAGAAACTGTGGTTGTGGGAGAGAAGTCGTTCCTTGAGGTCATGTCCCTCGTTTGGTTGAAGCTTGCAACGAATCCAAATTGCTATTCTTGCATCCTTGGGATCGCATGGGCTTTTATTTCTAACAGATGGCATCTGGAGCTGCCGGGCATATTAGAGGGTTCGATTCTTATAATGTCAAAAGCAGGAACAGGAACGGCCATGTTCAATATGGGAATATTCATGGCACTTCAAGAAAAGTTGATAGTATGTGGAACAAGCTTGACTGTGATGGGAATGGTATTGAAGTTTATTGCCGGACCGGCGGCCATGGCCATCGGCTCTATTGTCCTTGGCCTCCACGGTGATGTCCTCCGCGTAGCCATCATTCAGGCTGCTTTGCCACAATCGATTACTTCGTTCATTTTTGCTAAGGAATATGGATTACATGCAGATGTTCTAAGCACAGCGGTGATATTTGGGATGTTGGTCTCTCTACCTGTGCTCGTCGCCTACTACGCAGCTCTGGAGTTTATTCATTGA

Protein Analysis

351

Amino Acids

38.57

Weight (kDa)

6.58

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 344 3.9e-77 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16530 AT5G16530
fragaria_vesca FvH4_6g00660
malus_domestica MD04G1205100.v1.1 MD04G1243800.v1.1 MD12G1259500.v1.1
prunus_persica Prupe.6G360300_v2.0.a1 Prupe.6G360300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0448071
rosa_laevigata RLG00000025947
rosa_multiflora Rmu_sc0005969.1_g000026
rosa_roxburghii Rroxscaffold_6G00426230
rosa_rugosa Rorug02G0608100
rosa_samantha Rh3AG007600 Rh3BG007300 Rh3CG007300 Rh3DG007800
rosa_wichuraiana Rw3G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 707
AccB7I CCANNNNNTGG 1 cut(s) 429
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 2 cut(s) 186, 507
AccII CGCG 3 cut(s) 106, 218, 891
AciI CCGC 5 cut(s) 155, 218, 839, 889, 983
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 4 cut(s) 219, 732, 840, 846
AfaI GTAC 2 cut(s) 374, 501
AfiI CCNNNNNNNGG 3 cut(s) 235, 429, 588
AflIII ACRYGT 1 cut(s) 104
AgeI ACCGGT 1 cut(s) 207
AgsI TTSAA 7 cut(s) 196, 404, 419, 595, 742, 767, 820
AhlI ACTAGT 1 cut(s) 308
AjuI GAANNNNNNNTTGG 4 cut(s) 280, 312, 604, 636
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 5 cut(s) 291, 598, 676, 795, 1037
AluI AGCT 5 cut(s) 291, 598, 676, 795, 1037
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 2 cut(s) 137, 1009
AlwI GGATC 1 cut(s) 644
AoxI GGCC 7 cut(s) 177, 219, 267, 732, 840, 846, 868
ApeKI GCWGC 3 cut(s) 676, 908, 1034
AsiGI ACCGGT 1 cut(s) 207
Asp700I GAANNNNTTC 1 cut(s) 563
AspS9I GGNCC 4 cut(s) 178, 267, 300, 833
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 4 cut(s) 139, 154, 890, 997
AvaII GGWCC 2 cut(s) 300, 833
BalI TGGCCA 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 3 cut(s) 663, 895, 1046
BccI CCATC 5 cut(s) 431, 657, 799, 857, 905
BceAI ACGGC 2 cut(s) 192, 747
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 681
BcoDI GTCTC 2 cut(s) 137, 1009
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 1 cut(s) 309
BglI GCCNNNNNGGC 1 cut(s) 836
BisI GCNGC 6 cut(s) 155, 219, 677, 840, 909, 1035
BlsI GCNGC 6 cut(s) 156, 220, 678, 841, 910, 1036
Bme1390I CCNGG 1 cut(s) 681
Bme18I GGWCC 2 cut(s) 300, 833
BmgT120I GGNCC 4 cut(s) 178, 267, 300, 833
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 681
BmsI GCATC 3 cut(s) 109, 636, 676
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 2 cut(s) 363, 945
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 681
Bsa29I ATCGAT 1 cut(s) 923
BsaI GGTCTC 1 cut(s) 1009
BsaJI CCNNGG 5 cut(s) 408, 631, 843, 865, 874
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 429, 588
Bse118I RCCGGY 2 cut(s) 207, 835
Bse3DI GCAATG 2 cut(s) 45, 375
BseCI ATCGAT 1 cut(s) 923
BseDI CCNNGG 5 cut(s) 408, 631, 843, 865, 874
BseGI GGATG 2 cut(s) 627, 1002
BseLI CCNNNNNNNGG 3 cut(s) 235, 429, 588
BseMI GCAATG 2 cut(s) 45, 375
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 3 cut(s) 663, 895, 1046
Bsh1236I CGCG 3 cut(s) 106, 218, 891
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 7 cut(s) 179, 221, 269, 734, 842, 848, 870
BshTI ACCGGT 1 cut(s) 207
BshVI ATCGAT 1 cut(s) 923
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 1020
BsiSI CCGG 5 cut(s) 208, 471, 680, 831, 836
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 3 cut(s) 235, 429, 588
BsmAI GTCTC 2 cut(s) 137, 1009
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 565
BsnI GGCC 7 cut(s) 179, 221, 269, 734, 842, 848, 870
Bso31I GGTCTC 1 cut(s) 1009
Bsp1286I GDGCHC 1 cut(s) 1020
Bsp143I GATC 3 cut(s) 109, 391, 636
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 5 cut(s) 155, 218, 839, 889, 983
BspANI GGCC 7 cut(s) 179, 221, 269, 734, 842, 848, 870
BspDI ATCGAT 1 cut(s) 923
BspFNI CGCG 3 cut(s) 106, 218, 891
BspLI GGNNCC 1 cut(s) 45
BspPI GGATC 1 cut(s) 644
BspQI GCTCTTC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 1009
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 2 cut(s) 45, 375
BsrFI RCCGGY 2 cut(s) 207, 835
BssAI RCCGGY 2 cut(s) 207, 835
BssECI CCNNGG 5 cut(s) 408, 631, 843, 865, 874
BssMI GATC 3 cut(s) 109, 391, 636
BssT1I CCWWGG 3 cut(s) 631, 843, 865
Bst4CI ACNGT 5 cut(s) 131, 253, 544, 802, 878
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 7 cut(s) 67, 234, 256, 363, 454, 945, 974
BstDSI CCRYGG 2 cut(s) 843, 874
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 627, 1002
BstFNI CGCG 3 cut(s) 106, 218, 891
BstKTI GATC 3 cut(s) 112, 394, 639
BstMAI GTCTC 2 cut(s) 137, 1009
BstMBI GATC 3 cut(s) 109, 391, 636
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 6 cut(s) 266, 624, 673, 836, 845, 1031
BstNSI RCATGY 1 cut(s) 965
BstSCI CCNGG 1 cut(s) 679
BstUI CGCG 3 cut(s) 106, 218, 891
BstV1I GCAGC 3 cut(s) 663, 895, 1046
BstZI CGGCCG 1 cut(s) 219
Bsu15I ATCGAT 1 cut(s) 923
BsuRI GGCC 7 cut(s) 179, 221, 269, 734, 842, 848, 870
BsuTUI ATCGAT 1 cut(s) 923
BtgI CCRYGG 2 cut(s) 843, 874
BtgZI GCGATG 1 cut(s) 199
BtsCI GGATG 2 cut(s) 627, 1002
Cac8I GCNNGC 1 cut(s) 600
Cfr10I RCCGGY 2 cut(s) 207, 835
Cfr13I GGNCC 4 cut(s) 178, 267, 300, 833
ClaI ATCGAT 1 cut(s) 923
CpoI CGGWCCG 1 cut(s) 833
Csp6I GTAC 2 cut(s) 373, 500
CspAI ACCGGT 1 cut(s) 207
CspI CGGWCCG 1 cut(s) 833
CviAII CATG 8 cut(s) 182, 199, 577, 642, 736, 757, 844, 962
CviQI GTAC 2 cut(s) 373, 500
DdeI CTNAG 7 cut(s) 67, 234, 256, 363, 454, 945, 974
DpnI GATC 3 cut(s) 111, 393, 638
DpnII GATC 3 cut(s) 109, 391, 636
EaeI YGGCCR 4 cut(s) 219, 732, 840, 846
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 3 cut(s) 631, 843, 865
Eco31I GGTCTC 1 cut(s) 1009
Eco47I GGWCC 2 cut(s) 300, 833
Eco52I CGGCCG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoT14I CCWWGG 3 cut(s) 631, 843, 865
ErhI CCWWGG 3 cut(s) 631, 843, 865
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 8 cut(s) 185, 202, 580, 645, 739, 760, 847, 965
FaqI GGGAC 1 cut(s) 565
FatI CATG 8 cut(s) 181, 198, 576, 641, 735, 756, 843, 961
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 186, 507
Fnu4HI GCNGC 6 cut(s) 155, 219, 677, 840, 909, 1035
FokI GGATG 2 cut(s) 614, 1009
Fsp4HI GCNGC 6 cut(s) 155, 219, 677, 840, 909, 1035
FspBI CTAG 1 cut(s) 309
GluI GCNGC 6 cut(s) 155, 219, 677, 840, 909, 1035
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 7 cut(s) 179, 221, 269, 734, 842, 848, 870
HapII CCGG 5 cut(s) 208, 471, 680, 831, 836
Hin1II CATG 8 cut(s) 185, 202, 580, 645, 739, 760, 847, 965
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 2 cut(s) 596, 793
HinfI GANTC 2 cut(s) 607, 700
HpaII CCGG 5 cut(s) 208, 471, 680, 831, 836
HphI GGTGA 4 cut(s) 139, 154, 890, 997
Hpy166II GTNNAC 4 cut(s) 22, 174, 187, 508
Hpy188III TCNNGA 3 cut(s) 671, 767, 1040
Hpy8I GTNNAC 4 cut(s) 22, 174, 187, 508
Hpy99I CGWCG 2 cut(s) 500, 1025
HpyCH4III ACNGT 5 cut(s) 131, 253, 544, 802, 878
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 4 cut(s) 324, 602, 627, 965
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 624, 673, 836, 845, 1031
HpyF3I CTNAG 7 cut(s) 67, 234, 256, 363, 454, 945, 974
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 8 cut(s) 185, 202, 580, 645, 739, 760, 847, 965
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 109, 391, 636
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 3 cut(s) 663, 895, 1046
LweI GCATC 3 cut(s) 109, 636, 676
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 247
MalI GATC 3 cut(s) 111, 393, 638
MbiI CCGCTC 1 cut(s) 157
MboI GATC 3 cut(s) 109, 391, 636
MboII GAAGA 2 cut(s) 91, 151
MhlI GDGCHC 1 cut(s) 1020
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 3 cut(s) 7, 202, 613
MluI ACGCGT 1 cut(s) 104
MluNI TGGCCA 1 cut(s) 848
MnlI CCTC 7 cut(s) 239, 418, 565, 593, 685, 881, 896
Mox20I TGGCCA 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 563
MscI TGGCCA 1 cut(s) 848
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 3 cut(s) 879, 966, 983
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 5 cut(s) 208, 471, 680, 831, 836
MspR9I CCNGG 1 cut(s) 681
MvnI CGCG 3 cut(s) 106, 218, 891
MwoI GCNNNNNNNGC 6 cut(s) 266, 624, 673, 836, 845, 1031
NciI CCSGG 1 cut(s) 681
NcoI CCATGG 1 cut(s) 843
NdeII GATC 3 cut(s) 109, 391, 636
NlaIII CATG 8 cut(s) 185, 202, 580, 645, 739, 760, 847, 965
NlaIV GGNNCC 1 cut(s) 45
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 983
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 257, 929
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 2 cut(s) 607, 700
PflMI CCANNNNNTGG 1 cut(s) 429
PinAI ACCGGT 1 cut(s) 207
PkrI GCNGC 6 cut(s) 156, 220, 678, 841, 910, 1036
PsiI TTATAA 1 cut(s) 707
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 4 cut(s) 178, 267, 300, 833
RsaI GTAC 2 cut(s) 374, 501
RsaNI GTAC 2 cut(s) 373, 500
RseI CAYNNNNRTG 3 cut(s) 879, 966, 983
Rsr2I CGGWCCG 1 cut(s) 833
RsrII CGGWCCG 1 cut(s) 833
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 6 cut(s) 155, 219, 677, 840, 909, 1035
Sau3AI GATC 3 cut(s) 109, 391, 636
Sau96I GGNCC 4 cut(s) 178, 267, 300, 833
ScrFI CCNGG 1 cut(s) 681
SduI GDGCHC 1 cut(s) 1020
SfaNI GCATC 3 cut(s) 109, 636, 676
SinI GGWCC 2 cut(s) 300, 833
SmiMI CAYNNNNRTG 3 cut(s) 879, 966, 983
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SpeI ACTAGT 1 cut(s) 308
Sse9I AATT 3 cut(s) 7, 202, 613
SsiI CCGC 5 cut(s) 155, 218, 839, 889, 983
SspI AATATT 1 cut(s) 753
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 679
StyI CCWWGG 3 cut(s) 631, 843, 865
TaaI ACNGT 5 cut(s) 131, 253, 544, 802, 878
TaiI ACGT 1 cut(s) 501
TaqI TCGA 5 cut(s) 33, 186, 522, 698, 923
TasI AATT 3 cut(s) 7, 202, 613
TauI GCSGC 3 cut(s) 157, 221, 842
TfiI GAWTC 2 cut(s) 607, 700
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 3 cut(s) 676, 908, 1034
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 5 cut(s) 202, 215, 745, 925, 1040
TspGWI ACGGA 1 cut(s) 390
Van91I CCANNNNNTGG 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 300, 833
XagI CCTNNNNNAGG 1 cut(s) 233
XceI RCATGY 1 cut(s) 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XmiI GTMKAC 2 cut(s) 186, 507
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.