AT5G18820
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005115
Physical Location & Seq
Forward (+)
6271386 .. 6274599
3214 bp
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UTR
Exon/CDS
Intron
AT5G18820.3

Sequence Viewer

Length: 1728 bp
ATGTTCGCCGTATCACCGTCGTCATTCTCGCCGACAACCATTTCTCCGCGCCGTAGTGGACAGAGGAACGAACCTCGAAAATTCTCGGTGGTGAGAGCTGGAGCTAAGAGAATACTATACGGTAAAGATAGCAGAGAGAAACTCCAAGCCGGAATCGATAAGTTAGCTGATGCTGTTTCCATCACCTTAGGCCCTAGAGGGCGTAATGTAGTGCTTGCTGAAAAAGATACAATCAAAGTGATTAACGATGGTGTCACCATTGCTAAATCTATTGAGCTTCCTGACACTATTGAGAACGCTGGAGCAACATTAATCCAAGAGGTTGCGATTAAAATGAACGAATCAGCGGGTGACGGGACAACCACTGCAATCATTTTGGCTAGAGAAATGATCAAAGCTGGTTCTTTGGCTATTGCTTTTGGAGCTAATGCTGTTTCTGTGAAGAATGGAATGAATAAGACTGTTAAAGAGTTGGTCAGGGTGTTGCAGATGAAAAGTATTCCTGTTCAAGGGAAGAATGATATTAAAGCTGTGGCTTCAATCTCTGCTGGTAACGATGAATTTGTGGGGAATTTGATTGCTGAGACTGTGGAAAAGATTGGCCCTGATGGTGTCATCTCCATTGAATCATCTTCCACTTCAGAAACGTCTGTTATAGTTGAGGAAGGAATGAAGTTTGACAAGGGCTATATGTCGCCTCATTTCATCACAAATCAGGAGAAATCTACGGTGGAATTCGACAAAGCTAAAATTCTTGTGACGGATCAGAAAATCACTTCAGCCAAAGAATTAGTTCCGTTACTGGAGAAAACCTCACAACTGAGTGTTCCGCTCCTTATAATTGCAGAAGATATCTCTGCGGAAGTGCTTGAGATACTGGTGGTGAACAAGAAGCAAGGTTTGATCAATGTCGCAGTTGTGAAATGTCCAGGAATGTTGGATGGGAAAAAGGCTTTGCTACAAGACATTGCACTCATGACAGGAGCTGATTATCTTTCCGGAGATTTAGGCATGTCGCTCATGGGTGCAACTTCAGATCAGCTGGGTGTTTCTCGTAGAGTAGTAATTACAGCTAATTCAACAACTATAGTCGCAGACGCTTCTACTAAACCAGAGATTCAGGCAAGAATTGCTCAGATGAAGAAGGATCTAGCTGAGACAGATAATTCATATCTTTCAAAAAAAATAGCAGAGAGAATTGCTAAGCTCACAGGAGGCGTGGCTGTAATAAAGGTTGGAGGCCACACTGAAACAGAACTTGAGGACAGAAAACTAAGAATAGAGGACGCGAAGAATGCTACATTTGCAGCCATGAGAGAAGGTATAGTTCCAGGTGGTGGTGCTACTTATATCCATCTTTTAGATGAGATACCGAGAATCAAGAAGAATCTAATGGAAGATTCATACGAACAAATAGGTGCAGATATTGTAGCAATGGCACTCACTGCACCTGCAATGGCCATAGCAACCAATGCTGGGGTTGATGGATCAGTTGTGGTGCAGAAAACCAGAGAACTCGAATGGAGAAGTGGTTACAACGCAATGTCTGGAAAGTATGAGGATCTTCTAAACGCTGGAATCGCAGATCCTTGTCGGGTTTCAAGATTCGCCCTTCAAAACGCAGTTTCCGTTGCCGGCATTATTCTCACAACTCAAGCAGTACTCGTGGAGAAGATCAAGCAACCTAAACCTGCTGTTCCTCAAGTTCCTGGCATACCCACATCATAA

Protein Analysis

575

Amino Acids

61.19

Weight (kDa)

7.61

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 55 - 367 7.8e-36 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AarI CACCTGC 1 cut(s) 1459
Acc36I ACCTGC 2 cut(s) 1459, 1699
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccBSI CCGCTC 1 cut(s) 832
AccII CGCG 2 cut(s) 49, 1289
AccIII TCCGGA 1 cut(s) 998
AciI CCGC 4 cut(s) 47, 347, 830, 860
AclWI GGATC 5 cut(s) 771, 1155, 1495, 1569, 1580
AcoI YGGCCR 1 cut(s) 1458
AcsI RAATTY 5 cut(s) 80, 560, 571, 734, 750
AcuI CTGAAG 3 cut(s) 624, 762, 1017
AfaI GTAC 1 cut(s) 1662
AfiI CCNNNNNNNGG 3 cut(s) 509, 1337, 1476
AgsI TTSAA 7 cut(s) 509, 540, 626, 1080, 1179, 1602, 1616
AjnI CCWGG 3 cut(s) 928, 1330, 1708
Alw26I GTCTC 2 cut(s) 578, 1151
AlwI GGATC 5 cut(s) 771, 1155, 1495, 1569, 1580
AlwNI CAGNNNCTG 1 cut(s) 986
Aor13HI TCCGGA 1 cut(s) 998
AoxI GGCC 4 cut(s) 190, 601, 1240, 1458
ApeKI GCWGC 1 cut(s) 1307
ApoI RAATTY 5 cut(s) 80, 560, 571, 734, 750
AseI ATTAAT 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 191, 602
AsuHPI GGTGA 6 cut(s) 6, 103, 175, 247, 362, 895
AxyI CCTNAGG 1 cut(s) 187
BalI TGGCCA 1 cut(s) 1460
BauI CACGAG 1 cut(s) 1664
BbvI GCAGC 1 cut(s) 1319
BccI CCATC 6 cut(s) 188, 242, 602, 935, 1362, 1478
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 930, 1332, 1710
BclI TGATCA 2 cut(s) 390, 903
BcoDI GTCTC 2 cut(s) 578, 1151
BfaI CTAG 3 cut(s) 195, 381, 1151
BfmI CTRYAG 1 cut(s) 1086
BfuAI ACCTGC 2 cut(s) 1459, 1699
BisI GCNGC 1 cut(s) 1308
BlpI GCTNAGC 1 cut(s) 1203
BlsI GCNGC 1 cut(s) 1309
BmcAI AGTACT 1 cut(s) 1662
Bme1390I CCNGG 3 cut(s) 930, 1332, 1710
BmgT120I GGNCC 2 cut(s) 191, 602
BmrFI CCNGG 3 cut(s) 930, 1332, 1710
BmsI GCATC 1 cut(s) 160
BplI GAGNNNNNCTC 4 cut(s) 126, 158, 797, 829
BpmI CTGGAG 3 cut(s) 120, 321, 824
Bpu1102I GCTNAGC 1 cut(s) 1203
BpuEI CTTGAG 4 cut(s) 890, 1280, 1638, 1686
Bsa29I ATCGAT 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 998
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 1516, 1546
Bsc4I CCNNNNNNNGG 3 cut(s) 509, 1337, 1476
Bse118I RCCGGY 1 cut(s) 1634
Bse1I ACTGG 2 cut(s) 807, 882
Bse21I CCTNAGG 1 cut(s) 187
Bse3DI GCAATG 5 cut(s) 258, 966, 1440, 1461, 1548
BseAI TCCGGA 1 cut(s) 998
BseBI CCWGG 3 cut(s) 930, 1332, 1710
BseCI ATCGAT 1 cut(s) 156
BseGI GGATG 1 cut(s) 946
BseLI CCNNNNNNNGG 3 cut(s) 509, 1337, 1476
BseMI GCAATG 5 cut(s) 258, 966, 1440, 1461, 1548
BseMII CTCAG 4 cut(s) 573, 812, 1146, 1148
BseNI ACTGG 2 cut(s) 807, 882
BseXI GCAGC 1 cut(s) 1319
BseYI CCCAGC 2 cut(s) 1042, 1475
BsgI GTGCAG 3 cut(s) 1431, 1440, 1520
Bsh1236I CGCG 2 cut(s) 49, 1289
BshFI GGCC 4 cut(s) 192, 603, 1242, 1460
BshVI ATCGAT 1 cut(s) 156
BsiSI CCGG 3 cut(s) 150, 999, 1635
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 509, 1337, 1476
BsmAI GTCTC 2 cut(s) 578, 1151
BsmFI GGGAC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 1300
BsnI GGCC 4 cut(s) 192, 603, 1242, 1460
Bsp13I TCCGGA 1 cut(s) 998
Bsp143I GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
Bsp1720I GCTNAGC 1 cut(s) 1203
BspACI CCGC 4 cut(s) 47, 347, 830, 860
BspANI GGCC 4 cut(s) 192, 603, 1242, 1460
BspCNI CTCAG 4 cut(s) 574, 813, 1147, 1147
BspDI ATCGAT 1 cut(s) 156
BspEI TCCGGA 1 cut(s) 998
BspFNI CGCG 2 cut(s) 49, 1289
BspHI TCATGA 1 cut(s) 975
BspMI ACCTGC 2 cut(s) 1459, 1699
BspPI GGATC 5 cut(s) 771, 1155, 1495, 1569, 1580
BsrBI CCGCTC 1 cut(s) 832
BsrDI GCAATG 5 cut(s) 258, 966, 1440, 1461, 1548
BsrFI RCCGGY 1 cut(s) 1634
BsrI ACTGG 2 cut(s) 807, 882
BssAI RCCGGY 1 cut(s) 1634
BssMI GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
BssSI CACGAG 1 cut(s) 1664
Bst2BI CACGAG 1 cut(s) 1664
Bst2UI CCWGG 3 cut(s) 930, 1332, 1710
Bst4CI ACNGT 5 cut(s) 18, 122, 463, 589, 730
BstAPI GCANNNNNTGC 3 cut(s) 1130, 1445, 1472
BstC8I GCNNGC 2 cut(s) 216, 1636
BstDEI CTNAG 8 cut(s) 105, 187, 582, 821, 1134, 1155, 1203, 1274
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 946
BstFNI CGCG 2 cut(s) 49, 1289
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 9 cut(s) 393, 766, 906, 1039, 1150, 1490, 1564, 1588, 1677
BstMAI GTCTC 2 cut(s) 578, 1151
BstMBI GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
BstMWI GCNNNNNNNGC 7 cut(s) 422, 1130, 1295, 1304, 1445, 1472, 1580
BstNI CCWGG 3 cut(s) 930, 1332, 1710
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 3 cut(s) 928, 1330, 1708
BstSFI CTRYAG 1 cut(s) 1086
BstUI CGCG 2 cut(s) 49, 1289
BstV1I GCAGC 1 cut(s) 1319
BstX2I RGATCY 3 cut(s) 1147, 1561, 1585
BstYI RGATCY 3 cut(s) 1147, 1561, 1585
Bsu15I ATCGAT 1 cut(s) 156
Bsu36I CCTNAGG 1 cut(s) 187
BsuRI GGCC 4 cut(s) 192, 603, 1242, 1460
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 1 cut(s) 946
BtsI GCAGTG 2 cut(s) 363, 1443
BtsIMutI CAGTG 3 cut(s) 363, 1245, 1443
BveI ACCTGC 2 cut(s) 1459, 1699
Cac8I GCNNGC 2 cut(s) 216, 1636
CaiI CAGNNNCTG 1 cut(s) 986
CciI TCATGA 1 cut(s) 975
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 1634
Cfr13I GGNCC 2 cut(s) 191, 602
ClaI ATCGAT 1 cut(s) 156
CseI GACGC 2 cut(s) 1106, 1295
Csp6I GTAC 1 cut(s) 1661
CviAII CATG 4 cut(s) 976, 1012, 1021, 1312
CviQI GTAC 1 cut(s) 1661
DdeI CTNAG 8 cut(s) 105, 187, 582, 821, 1134, 1155, 1203, 1274
DpnI GATC 9 cut(s) 392, 765, 905, 1038, 1149, 1489, 1563, 1587, 1676
DpnII GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
EaeI YGGCCR 1 cut(s) 1458
Eco32I GATATC 1 cut(s) 853
Eco57I CTGAAG 3 cut(s) 624, 762, 1017
Eco81I CCTNAGG 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 928, 1330, 1708
EcoRV GATATC 1 cut(s) 853
FaeI CATG 4 cut(s) 979, 1015, 1024, 1315
FaqI GGGAC 1 cut(s) 370
FatI CATG 4 cut(s) 975, 1011, 1020, 1311
FauI CCCGC 1 cut(s) 340
FbaI TGATCA 2 cut(s) 390, 903
Fnu4HI GCNGC 1 cut(s) 1308
FokI GGATG 1 cut(s) 953
Fsp4HI GCNGC 1 cut(s) 1308
FspBI CTAG 3 cut(s) 195, 381, 1151
GlaI GCGC 1 cut(s) 50
GluI GCNGC 1 cut(s) 1308
GsaI CCCAGC 2 cut(s) 1046, 1479
GsuI CTGGAG 3 cut(s) 120, 321, 824
HaeIII GGCC 4 cut(s) 192, 603, 1242, 1460
HapII CCGG 3 cut(s) 150, 999, 1635
HgaI GACGC 2 cut(s) 1106, 1295
HhaI GCGC 1 cut(s) 51
Hin1II CATG 4 cut(s) 979, 1015, 1024, 1315
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HinfI GANTC 9 cut(s) 153, 341, 626, 1117, 1377, 1387, 1400, 1578, 1605
HpaII CCGG 3 cut(s) 150, 999, 1635
HphI GGTGA 6 cut(s) 6, 103, 175, 247, 362, 895
Hpy166II GTNNAC 2 cut(s) 59, 886
Hpy188I TCNGA 4 cut(s) 643, 768, 1036, 1137
Hpy188III TCNNGA 7 cut(s) 281, 716, 976, 999, 1381, 1548, 1602
Hpy8I GTNNAC 2 cut(s) 59, 886
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 4 cut(s) 659, 1138, 1313, 1622
HpyCH4III ACNGT 5 cut(s) 18, 122, 463, 589, 730
HpyCH4IV ACGT 1 cut(s) 647
HpyF10VI GCNNNNNNNGC 7 cut(s) 422, 1130, 1295, 1304, 1445, 1472, 1580
HpyF3I CTNAG 8 cut(s) 105, 187, 582, 821, 1134, 1155, 1203, 1274
HpySE526I ACGT 1 cut(s) 647
Hsp92II CATG 4 cut(s) 979, 1015, 1024, 1315
HspAI GCGC 1 cut(s) 49
Kpn2I TCCGGA 1 cut(s) 998
KroI GCCGGC 1 cut(s) 1634
KroNI GCCGGC 1 cut(s) 1636
Ksp22I TGATCA 2 cut(s) 390, 903
Kzo9I GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
LmnI GCTCC 5 cut(s) 101, 302, 422, 837, 983
Lsp1109I GCAGC 1 cut(s) 1319
LweI GCATC 1 cut(s) 160
MaeI CTAG 3 cut(s) 195, 381, 1151
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 6 cut(s) 253, 350, 551, 757, 798, 1532
MalI GATC 9 cut(s) 392, 765, 905, 1038, 1149, 1489, 1563, 1587, 1676
MbiI CCGCTC 1 cut(s) 832
MboI GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
MflI RGATCY 3 cut(s) 1147, 1561, 1585
MlsI TGGCCA 1 cut(s) 1460
MluNI TGGCCA 1 cut(s) 1460
MmeI TCCRAC 2 cut(s) 918, 1216
Mox20I TGGCCA 1 cut(s) 1460
MroI TCCGGA 1 cut(s) 998
MroNI GCCGGC 1 cut(s) 1634
MroXI GAANNNNTTC 1 cut(s) 792
MscI TGGCCA 1 cut(s) 1460
MseI TTAA 5 cut(s) 243, 311, 330, 465, 525
Msp20I TGGCCA 1 cut(s) 1460
MspA1I CMGCKG 2 cut(s) 347, 1042
MspI CCGG 3 cut(s) 150, 999, 1635
MspR9I CCNGG 3 cut(s) 930, 1332, 1710
Mva1269I GAATGC 1 cut(s) 1300
MvaI CCWGG 3 cut(s) 930, 1332, 1710
MvnI CGCG 2 cut(s) 49, 1289
MwoI GCNNNNNNNGC 7 cut(s) 422, 1130, 1295, 1304, 1445, 1472, 1580
NaeI GCCGGC 1 cut(s) 1636
NdeII GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
NgoMIV GCCGGC 1 cut(s) 1634
NlaIII CATG 4 cut(s) 979, 1015, 1024, 1315
NmuCI GTSAC 3 cut(s) 253, 350, 757
NspI RCATGY 1 cut(s) 1015
PagI TCATGA 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 1459
PcsI WCGNNNNNNNCGW 1 cut(s) 1626
PctI GAATGC 1 cut(s) 1300
PdiI GCCGGC 1 cut(s) 1636
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 9 cut(s) 153, 341, 626, 1117, 1377, 1387, 1400, 1578, 1605
PflMI CCANNNNNTGG 1 cut(s) 1337
PfoI TCCNGGA 1 cut(s) 928
PkrI GCNGC 1 cut(s) 1309
PshBI ATTAAT 1 cut(s) 311
PsiI TTATAA 1 cut(s) 839
Psp6I CCWGG 3 cut(s) 928, 1330, 1708
PspFI CCCAGC 2 cut(s) 1042, 1475
PspGI CCWGG 3 cut(s) 928, 1330, 1708
PspPI GGNCC 2 cut(s) 191, 602
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 3 cut(s) 1147, 1561, 1585
PvuII CAGCTG 1 cut(s) 1042
RsaI GTAC 1 cut(s) 1662
RsaNI GTAC 1 cut(s) 1661
SaqAI TTAA 5 cut(s) 243, 311, 330, 465, 525
SatI GCNGC 1 cut(s) 1308
Sau3AI GATC 9 cut(s) 390, 763, 903, 1036, 1147, 1487, 1561, 1585, 1674
Sau96I GGNCC 2 cut(s) 191, 602
ScaI AGTACT 1 cut(s) 1662
ScrFI CCNGG 3 cut(s) 930, 1332, 1710
SfaNI GCATC 1 cut(s) 160
SfcI CTRYAG 1 cut(s) 1086
SmlI CTYRAG 4 cut(s) 869, 1259, 1653, 1701
SmoI CTYRAG 4 cut(s) 869, 1259, 1653, 1701
SsiI CCGC 4 cut(s) 47, 347, 830, 860
SspMI CTAG 3 cut(s) 195, 381, 1151
StyD4I CCNGG 3 cut(s) 928, 1330, 1708
TaaI ACNGT 5 cut(s) 18, 122, 463, 589, 730
TaiI ACGT 1 cut(s) 650
TaqI TCGA 4 cut(s) 76, 156, 738, 1518
TatI WGTACW 1 cut(s) 1660
TfiI GAWTC 9 cut(s) 153, 341, 626, 1117, 1377, 1387, 1400, 1578, 1605
Tru1I TTAA 5 cut(s) 243, 311, 330, 465, 525
Tru9I TTAA 5 cut(s) 243, 311, 330, 465, 525
TscAI CASTG 3 cut(s) 370, 1252, 1450
TseFI GTSAC 3 cut(s) 253, 350, 757
TseI GCWGC 1 cut(s) 1307
Tsp45I GTSAC 3 cut(s) 253, 350, 757
TspDTI ATGAA 9 cut(s) 350, 467, 506, 573, 686, 694, 1154, 1158, 1392
TspGWI ACGGA 3 cut(s) 776, 786, 1618
TspRI CASTG 3 cut(s) 370, 1252, 1450
Van91I CCANNNNNTGG 1 cut(s) 1337
VspI ATTAAT 1 cut(s) 311
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 5 cut(s) 80, 560, 571, 734, 750
XceI RCATGY 1 cut(s) 1015
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 3 cut(s) 195, 381, 1151
ZrmI AGTACT 1 cut(s) 1662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.