FvH4_3g12390
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005115
Physical Location & Seq
Forward (+)
7352470 .. 7357047
4578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12390.t9

Sequence Viewer

Length: 1758 bp
ATGTCTGTCTCTCTGTCTTCCTCTCAGCTCTTCTTTTACACACCTCTCTTCTCTCTGCAGAGCTTTTCTCAGAAACAAAGAGTAGGTGGGTTTTGGAGAAAGCCACAAGCTTTGAGGAGCCTTGAGGTGAGAGCAGGCCCAAAGAAGATATCTTTTGGTAAAGAATGCAGGGAGGGATTGCAAATTGGGATAGATAAGCTTGCTGATGCTGTTTCTCTTACATTAGGACCTAAAGGACGTAATGTTGTTCTTTCTGAGTCTGATAAAGTGAGAGTGATTAATGATGGGGTGACAATTGTTCAGTCCATAGAGCTAGCAGATACAATTGAGAACGCGGGAGCAATGCTAATCCAAGAGGTTGCAAGTAAAATGAATAATTTGGCCGGTGATGGTACTACCACTGCAGTTATTTTGACACGAGAAATGATCAGAGGTGGATTATTAGCAGTAGCTTTTGGGGCTAACCCTATTTCCTTGAAGAAAGGAATGGAGAAGACTGTTACTGAACTGCTCGAGGTTTTGAAGGAGAAAAGTCTTCCTGTGAAAGGAAGGAAGGATATTAAAGCTGTGGCTTCAATTTCTGCCGGAAATGATGAATTCATTGGGAACTTGATTGCAGAAGCTATAGAAAAGATTGGTCCTGATGGAGTGATCTCGATTGAGTCGTCCTCATCATTTGAAACGTCTGTTTTAGTCGAAGAAGGACTCAAGTTTGACAAGGGTTACATGTCCTCCCACTTCATTACTAATCAAGAAAGATCTGTTGTGGAGTTTGACAAGGCTAAGGTCCTGGTAACTGATCAAAAGATTTCATCTGTTAAAGAAATTGTTCCTTTGCTGGAAAAGATAACCCAATTGAGTATCCCGCTGCTAATCATTGCAGAGGATATCTCAAAGCCAGTGCTGGAAACACTGGTGGTGAATAAAATGCAGGGTTTACTTAATGTTGCTGTTGTTAAGTGCCCTGGATTTGGAGAGCGGAAGAAAGCTCTTTTGCAAGATATTGCACTGATGACAGGTGCTGATTTTCTCTCTGGAGATTTTGGTTTTACCCTTGACAGTGCAACATCAGATCAACTTGGAATTGCAAGAAAGGTGACAATAACAAGTAATTCAACAACCATAGTTGCTGACCCTTCTACCAAAGCAGAGATTCAGGCAAGAATATTGCAGATCAAGAAGGATCTAGCAGAAACTGAAAATGCAAACCTGTCGAGAAAGCTCTCTGAAAGAATTGCTAAACTCTGTGGTGGTATTGCTGTAATTAAGGTAGGAGCACATACTGAGGTGGAACTTGAAGATCGCAAACTCAGAATTGAGGATGCAAAGAATGCCACATTTGCTGCTATGGAGGAAGGGGTAGTGCCTGGTGGTGGTGCCACTTATGTACATCTGTCAGAATCGATTCCTGTCATAAAGAATTCTATGGAAGATTCGGATGAGCAGTTTGGTGCCGACATTATAGCCAAGGCTCTCCTTGCTCCTGCAAAATCAATTGCAACTAATGCTGGGGATGATGGAGAAGTAGTTGTGGAGAAGACAAAAGATTGTGATTGGAGAATTGGGTACAATGCCATGACTGGCAGGTATGAAGATCTTCCAGATGCAGGAGTTTTAGATCCTTGTCGAGTTTCAAGATGTGCCCTTCAAATTGCAGTCTCCATTGCTGGGGTGGTTCTCACTACTCAAGCTGTATTGGTGGAGAAAACCAAGACGAAAAAGCCAGCAATTCCTTCTGTTCCAGGGATAACTCCTTAG

Protein Analysis

586

Amino Acids

62.66

Weight (kDa)

5.69

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 2 - 262 7.2e-30 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1575
AccB1I GGYRCC 2 cut(s) 1376, 1451
AccBSI CCGCTC 1 cut(s) 979
AccII CGCG 1 cut(s) 335
AciI CCGC 3 cut(s) 335, 866, 979
AclWI GGATC 2 cut(s) 1191, 1613
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 2 cut(s) 596, 1420
AfaI GTAC 3 cut(s) 394, 1389, 1568
AfiI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 8 cut(s) 478, 523, 576, 680, 1116, 1298, 1635, 1649
AjnI CCWGG 4 cut(s) 789, 964, 1366, 1741
Alw21I GWGCWC 1 cut(s) 1279
Alw26I GTCTC 2 cut(s) 13, 1663
AlwI GGATC 2 cut(s) 1191, 1613
AlwNI CAGNNNCTG 2 cut(s) 1022, 1196
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 2 cut(s) 136, 381
ApeKI GCWGC 2 cut(s) 868, 1343
ApoI RAATTY 2 cut(s) 596, 1420
AseI ATTAAT 1 cut(s) 279
Asp700I GAANNNNTTC 3 cut(s) 828, 1404, 1596
AspS9I GGNCC 4 cut(s) 137, 227, 638, 787
AsuHPI GGTGA 5 cut(s) 139, 301, 398, 931, 1108
AsuNHI GCTAGC 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 3 cut(s) 227, 638, 787
BaeGI GKGCMC 2 cut(s) 965, 1645
BanI GGYRCC 2 cut(s) 1376, 1451
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 4 cut(s) 9, 500, 527, 1544
Bbv12I GWGCWC 1 cut(s) 1279
BbvI GCAGC 2 cut(s) 855, 1330
BccI CCATC 4 cut(s) 278, 383, 638, 1511
BcgI CGANNNNNNTGC 2 cut(s) 1194, 1228
BciT130I CCWGG 4 cut(s) 791, 966, 1368, 1743
BciVI GTATCC 1 cut(s) 872
BclI TGATCA 2 cut(s) 426, 799
BcoDI GTCTC 2 cut(s) 13, 1663
BfaI CTAG 2 cut(s) 314, 1187
BfmI CTRYAG 3 cut(s) 56, 402, 624
BfuAI ACCTGC 1 cut(s) 1575
BfuI GTATCC 1 cut(s) 872
BglII AGATCT 2 cut(s) 758, 1594
BisI GCNGC 2 cut(s) 869, 1344
BlsI GCNGC 2 cut(s) 870, 1345
Bme1390I CCNGG 4 cut(s) 791, 966, 1368, 1743
Bme18I GGWCC 3 cut(s) 227, 638, 787
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 4 cut(s) 137, 227, 638, 787
BmiI GGNNCC 3 cut(s) 119, 1378, 1453
BmrFI CCNGG 4 cut(s) 791, 966, 1368, 1743
BmsI GCATC 3 cut(s) 196, 1312, 1594
BmtI GCTAGC 1 cut(s) 317
BpiI GAAGAC 4 cut(s) 9, 500, 527, 1544
BplI GAGNNNNNCTC 6 cut(s) 52, 84, 653, 685, 875, 907
BpmI CTGGAG 1 cut(s) 1056
Bpu10I CCTNAGC 1 cut(s) 783
BpuEI CTTGAG 3 cut(s) 143, 692, 1671
Bsa29I ATCGAT 1 cut(s) 1403
BsaJI CCNNGG 3 cut(s) 964, 1467, 1742
BsaXI ACNNNNNCTCC 4 cut(s) 716, 746, 1512, 1542
Bsc4I CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 3 cut(s) 899, 918, 1585
Bse3DI GCAATG 3 cut(s) 348, 876, 1662
BseBI CCWGG 4 cut(s) 791, 966, 1368, 1743
BseCI ATCGAT 1 cut(s) 1403
BseDI CCNNGG 3 cut(s) 964, 1467, 1742
BseGI GGATG 3 cut(s) 1327, 1444, 1519
BseLI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
BseMI GCAATG 3 cut(s) 348, 876, 1662
BseMII CTCAG 5 cut(s) 38, 83, 246, 1275, 1324
BseNI ACTGG 3 cut(s) 899, 918, 1585
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 2 cut(s) 965, 1645
BseXI GCAGC 2 cut(s) 855, 1330
BseYI CCCAGC 2 cut(s) 1508, 1667
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 2 cut(s) 138, 383
BshNI GGYRCC 2 cut(s) 1376, 1451
BshVI ATCGAT 1 cut(s) 1403
BsiHKAI GWGCWC 1 cut(s) 1279
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 2 cut(s) 384, 585
BslI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
BsmAI GTCTC 2 cut(s) 13, 1663
BsmI GAATGC 2 cut(s) 170, 1336
BsnI GGCC 2 cut(s) 138, 383
BsoBI CYCGRG 1 cut(s) 512
Bsp1286I GDGCHC 3 cut(s) 965, 1279, 1645
Bsp1407I TGTACA 1 cut(s) 1387
BspACI CCGC 3 cut(s) 335, 866, 979
BspANI GGCC 2 cut(s) 138, 383
BspCNI CTCAG 5 cut(s) 37, 82, 247, 1276, 1323
BspDI ATCGAT 1 cut(s) 1403
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 3 cut(s) 119, 1378, 1453
BspMAI CTGCAG 2 cut(s) 60, 406
BspMI ACCTGC 1 cut(s) 1575
BspOI GCTAGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 1191, 1613
BspQI GCTCTTC 1 cut(s) 35
BspT107I GGYRCC 2 cut(s) 1376, 1451
BsrBI CCGCTC 1 cut(s) 979
BsrDI GCAATG 3 cut(s) 348, 876, 1662
BsrFI RCCGGY 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 1387
BsrI ACTGG 3 cut(s) 899, 918, 1585
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 3 cut(s) 964, 1467, 1742
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 1467
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 4 cut(s) 791, 966, 1368, 1743
Bst4CI ACNGT 2 cut(s) 499, 1061
Bst6I CTCTTC 2 cut(s) 35, 53
BstAPI GCANNNNNTGC 2 cut(s) 1331, 1505
BstAUI TGTACA 1 cut(s) 1387
BstC8I GCNNGC 4 cut(s) 136, 201, 315, 1725
BstDEI CTNAG 7 cut(s) 24, 69, 255, 783, 1284, 1310, 1755
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 1327, 1444, 1519
BstFNI CGCG 1 cut(s) 335
BstMAI GTCTC 2 cut(s) 13, 1663
BstMWI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
BstNI CCWGG 4 cut(s) 791, 966, 1368, 1743
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 4 cut(s) 789, 964, 1366, 1741
BstSFI CTRYAG 3 cut(s) 56, 402, 624
BstSLI GKGCMC 2 cut(s) 965, 1645
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 2 cut(s) 855, 1330
BstV2I GAAGAC 4 cut(s) 9, 500, 527, 1544
BstX2I RGATCY 4 cut(s) 758, 1183, 1594, 1618
BstYI RGATCY 4 cut(s) 758, 1183, 1594, 1618
Bsu15I ATCGAT 1 cut(s) 1403
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 2 cut(s) 138, 383
BsuTUI ATCGAT 1 cut(s) 1403
BtsCI GGATG 3 cut(s) 1327, 1444, 1519
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 5 cut(s) 399, 906, 911, 1007, 1066
BveI ACCTGC 1 cut(s) 1575
Cac8I GCNNGC 4 cut(s) 136, 201, 315, 1725
CaiI CAGNNNCTG 2 cut(s) 1022, 1196
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 137, 227, 638, 787
ClaI ATCGAT 1 cut(s) 1403
Csp6I GTAC 3 cut(s) 393, 1388, 1567
CspCI CAANNNNNGTGG 2 cut(s) 93, 128
CviAII CATG 2 cut(s) 727, 1576
CviQI GTAC 3 cut(s) 393, 1388, 1567
DdeI CTNAG 7 cut(s) 24, 69, 255, 783, 1284, 1310, 1755
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 2 cut(s) 35, 53
EarI CTCTTC 2 cut(s) 35, 53
Eco130I CCWWGG 1 cut(s) 1467
Eco32I GATATC 2 cut(s) 150, 889
Eco47I GGWCC 3 cut(s) 227, 638, 787
Eco88I CYCGRG 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoO109I RGGNCCY 2 cut(s) 227, 787
EcoRI GAATTC 2 cut(s) 596, 1420
EcoRII CCWGG 4 cut(s) 789, 964, 1366, 1741
EcoRV GATATC 2 cut(s) 150, 889
EcoT14I CCWWGG 1 cut(s) 1467
ErhI CCWWGG 1 cut(s) 1467
FaeI CATG 2 cut(s) 730, 1579
FatI CATG 2 cut(s) 726, 1575
FauI CCCGC 2 cut(s) 328, 873
FbaI TGATCA 2 cut(s) 426, 799
Fnu4HI GCNGC 2 cut(s) 869, 1344
FokI GGATG 3 cut(s) 1334, 1451, 1526
Fsp4HI GCNGC 2 cut(s) 869, 1344
FspBI CTAG 2 cut(s) 314, 1187
GluI GCNGC 2 cut(s) 869, 1344
GsaI CCCAGC 2 cut(s) 1512, 1671
GsuI CTGGAG 1 cut(s) 1056
HaeIII GGCC 2 cut(s) 138, 383
HapII CCGG 2 cut(s) 384, 585
Hin1II CATG 2 cut(s) 730, 1579
HindIII AAGCTT 2 cut(s) 108, 197
HinfI GANTC 7 cut(s) 257, 662, 705, 1153, 1400, 1405, 1433
HpaII CCGG 2 cut(s) 384, 585
HphI GGTGA 5 cut(s) 139, 301, 398, 931, 1108
Hpy166II GTNNAC 1 cut(s) 938
Hpy188I TCNGA 9 cut(s) 72, 256, 262, 431, 1072, 1228, 1313, 1399, 1438
Hpy188III TCNNGA 8 cut(s) 641, 655, 752, 1035, 1177, 1215, 1601, 1635
Hpy8I GTNNAC 1 cut(s) 938
HpyAV CCTTC 9 cut(s) 517, 543, 547, 695, 1146, 1174, 1349, 1655, 1743
HpyCH4III ACNGT 2 cut(s) 499, 1061
HpyCH4IV ACGT 2 cut(s) 238, 683
HpyF10VI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
HpyF3I CTNAG 7 cut(s) 24, 69, 255, 783, 1284, 1310, 1755
HpySE526I ACGT 2 cut(s) 238, 683
Hsp92II CATG 2 cut(s) 730, 1579
Ksp22I TGATCA 2 cut(s) 426, 799
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 4 cut(s) 117, 338, 1274, 1486
Lsp1109I GCAGC 2 cut(s) 855, 1330
LweI GCATC 3 cut(s) 196, 1312, 1594
MaeI CTAG 2 cut(s) 314, 1187
MaeII ACGT 2 cut(s) 238, 683
MaeIII GTNAC 5 cut(s) 289, 499, 722, 793, 1096
MbiI CCGCTC 1 cut(s) 979
MfeI CAATTG 4 cut(s) 294, 324, 854, 1494
MflI RGATCY 4 cut(s) 758, 1183, 1594, 1618
MhlI GDGCHC 3 cut(s) 965, 1279, 1645
MlyI GAGTC 3 cut(s) 266, 671, 699
MroXI GAANNNNTTC 3 cut(s) 828, 1404, 1596
MseI TTAA 6 cut(s) 279, 561, 819, 942, 957, 1266
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 2 cut(s) 384, 585
MspR9I CCNGG 4 cut(s) 791, 966, 1368, 1743
MunI CAATTG 4 cut(s) 294, 324, 854, 1494
Mva1269I GAATGC 2 cut(s) 170, 1336
MvaI CCWGG 4 cut(s) 791, 966, 1368, 1743
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 2 cut(s) 730, 1579
NlaIV GGNNCC 3 cut(s) 119, 1378, 1453
NmuCI GTSAC 2 cut(s) 289, 1096
NspI RCATGY 1 cut(s) 730
PaeR7I CTCGAG 1 cut(s) 512
PciI ACATGT 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 662
PctI GAATGC 2 cut(s) 170, 1336
PdmI GAANNNNTTC 3 cut(s) 828, 1404, 1596
PfeI GAWTC 4 cut(s) 1153, 1400, 1405, 1433
PkrI GCNGC 2 cut(s) 870, 1345
PleI GAGTC 3 cut(s) 265, 670, 699
PpsI GAGTC 3 cut(s) 265, 670, 699
PpuMI RGGWCCY 2 cut(s) 227, 787
PscI ACATGT 1 cut(s) 726
PshBI ATTAAT 1 cut(s) 279
Psp5II RGGWCCY 2 cut(s) 227, 787
Psp6I CCWGG 4 cut(s) 789, 964, 1366, 1741
PspFI CCCAGC 2 cut(s) 1508, 1667
PspGI CCWGG 4 cut(s) 789, 964, 1366, 1741
PspN4I GGNNCC 3 cut(s) 119, 1378, 1453
PspPI GGNCC 4 cut(s) 137, 227, 638, 787
PspPPI RGGWCCY 2 cut(s) 227, 787
PspXI VCTCGAGB 1 cut(s) 512
PstI CTGCAG 2 cut(s) 60, 406
PstNI CAGNNNCTG 2 cut(s) 1022, 1196
PsuI RGATCY 4 cut(s) 758, 1183, 1594, 1618
RsaI GTAC 3 cut(s) 394, 1389, 1568
RsaNI GTAC 3 cut(s) 393, 1388, 1567
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 6 cut(s) 279, 561, 819, 942, 957, 1266
SatI GCNGC 2 cut(s) 869, 1344
Sau96I GGNCC 4 cut(s) 137, 227, 638, 787
SchI GAGTC 3 cut(s) 266, 671, 699
ScrFI CCNGG 4 cut(s) 791, 966, 1368, 1743
SduI GDGCHC 3 cut(s) 965, 1279, 1645
SfaNI GCATC 3 cut(s) 196, 1312, 1594
SfcI CTRYAG 3 cut(s) 56, 402, 624
Sfr274I CTCGAG 1 cut(s) 512
SinI GGWCC 3 cut(s) 227, 638, 787
SlaI CTCGAG 1 cut(s) 512
SmlI CTYRAG 4 cut(s) 122, 512, 707, 1686
SmoI CTYRAG 4 cut(s) 122, 512, 707, 1686
SsiI CCGC 3 cut(s) 335, 866, 979
SspI AATATT 1 cut(s) 1167
SspMI CTAG 2 cut(s) 314, 1187
StyD4I CCNGG 4 cut(s) 789, 964, 1366, 1741
StyI CCWWGG 1 cut(s) 1467
TaaI ACNGT 2 cut(s) 499, 1061
TaiI ACGT 2 cut(s) 241, 686
TaqI TCGA 6 cut(s) 513, 656, 696, 1214, 1403, 1627
TatI WGTACW 1 cut(s) 1387
TfiI GAWTC 4 cut(s) 1153, 1400, 1405, 1433
Tru1I TTAA 6 cut(s) 279, 561, 819, 942, 957, 1266
Tru9I TTAA 6 cut(s) 279, 561, 819, 942, 957, 1266
TscAI CASTG 5 cut(s) 406, 906, 918, 1014, 1066
TseFI GTSAC 2 cut(s) 289, 1096
TseI GCWGC 2 cut(s) 868, 1343
Tsp45I GTSAC 2 cut(s) 289, 1096
TspDTI ATGAA 6 cut(s) 386, 589, 609, 730, 801, 1605
TspRI CASTG 5 cut(s) 406, 906, 918, 1014, 1066
VpaK11BI GGWCC 3 cut(s) 227, 638, 787
VspI ATTAAT 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 2 cut(s) 596, 1420
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XhoI CTCGAG 1 cut(s) 512
XmnI GAANNNNTTC 3 cut(s) 828, 1404, 1596
XspI CTAG 2 cut(s) 314, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.