Rh5CG159300
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005115
Physical Location & Seq
Forward (+)
14654615 .. 14659171
4557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG159300.1

Sequence Viewer

Length: 1305 bp
ATGTCTCTCTCTCTGTCTTCCTCTCAGCTCTTCCTTTACACACCTCTCTTCTCTCTGCAGAGCTCCTCCCAGAAGCGAAGAGTATGTGGGTTTTGGAAAAAGCCACAGGCTTTGAGGAGTCTTGTGGTGAGAGCAGGCCCAAAGAAGATATCTTTTGGTAAAGAATGCAGAGAGGGATTGCAAATTGGGATAGATAAGCTTGCTGATGCTGTTTCTCTTACATTAGGACCTAGAGGACGTAATGTTGTTCTTTCTGAGTCTGAAAAAGTGAGAGTGATTAATGATGGTGTAACAATTGTTCAGTCCATAGAGCTAGCAGATACAATTGAGAACGCGGGAGCAATGCTAATCCAAGAGGTTGCAAGTAAAATGAATAATTTGGCCGGTGATGGTACTACCACTGCGATCATTTTGACACGAGAAATGATCAAAGGTGGATTATTGGCAGTGGCTTTTGGGGCTAACCCAATTTCTTTGAAGAAAGGAATGGAGAAGACTGTTAGTGAATTGGTCAAGGTGTTAAAGGAGAAAAGTCTTCCCGTAAAAGGAAGGAATGATATTAAAGCTGTGGCTTCAATTTCTGCCGGAAATGATGAATTCATAGGAAACTTGATTGCGGAAGCTATAGAAAAGATTGGTCCTGATGGAGTAATCTCAATTGAGTCGTCCTCATCATTTGAAACATCTGTTTTAGTTGAAGAAGGACTAAAGTTTGAAAAGGGTTACATGTCCTCCCACTTCATTACTAATCAAGAAAGATCTATTGTGGAGTTTGACAAGGCTAAGGTCCTGGTAACTGATCAAAAGATTTCAACTGTTAAAGAAATTGTTCCTTTGCTGGAAAAGATAACCCAATTGAGTATCCCGCTGCTAATCATTGCAGAGGATATCTCAAGGCCAGTACTGGAGACACTAGTGGTGAATAAAATGCAGGGTTTACTTAATGTTGCTGTTGTTAAGTGCCCTGGATTTGGAGAGCGGAAGAAAGCTCTTTTGCAAGATATTGCACTTATGACAGGTGCTGATTTTCTCTCTGGAGATTTTGGTTTTACCCTTGACAGTGCAACATCAGATCAACTTGGAATTGCAAGAAAGGTGACAATAACAAGTAATTCAACAACCATAGTTGCTGACCCTTCTACCAAAGCAGAGATTCAGGCAAGAATATTGCAGATCAAGAAGGATCTAGCAGAAACTGAAAATGCAAACCTGTCGAGAAAGCTCTCCGAAAGAATTGCTAAACTCTGTGGTGGAGTTGCCGTAATTAAGGTCTGCAATATGATAGACCTACAACACTTGATGTGA

Protein Analysis

434

Amino Acids

46.83

Weight (kDa)

8.77

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 67 - 380 2.4e-42 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 979
AccII CGCG 1 cut(s) 335
AciI CCGC 4 cut(s) 335, 617, 866, 979
AclWI GGATC 1 cut(s) 1191
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 1 cut(s) 596
AfaI GTAC 2 cut(s) 394, 903
AfiI CCNNNNNNNGG 2 cut(s) 545, 971
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 7 cut(s) 478, 576, 680, 698, 716, 813, 1116
AhlI ACTAGT 1 cut(s) 913
AjnI CCWGG 2 cut(s) 789, 964
AluBI AGCT 8 cut(s) 28, 63, 199, 313, 566, 623, 989, 1222
AluI AGCT 8 cut(s) 28, 63, 199, 313, 566, 623, 989, 1222
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 2 cut(s) 9, 902
AlwI GGATC 1 cut(s) 1191
AlwNI CAGNNNCTG 2 cut(s) 1022, 1196
AoxI GGCC 3 cut(s) 136, 381, 896
ApeKI GCWGC 1 cut(s) 868
ApoI RAATTY 1 cut(s) 596
AseI ATTAAT 1 cut(s) 279
Asp700I GAANNNNTTC 1 cut(s) 828
AspS9I GGNCC 4 cut(s) 137, 227, 638, 787
AsuHPI GGTGA 4 cut(s) 139, 398, 931, 1108
AsuNHI GCTAGC 1 cut(s) 313
AvaII GGWCC 3 cut(s) 227, 638, 787
BaeGI GKGCMC 1 cut(s) 965
BanII GRGCYC 1 cut(s) 65
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 3 cut(s) 9, 500, 527
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 855
BccI CCATC 3 cut(s) 278, 383, 638
BceAI ACGGC 1 cut(s) 1244
BcgI CGANNNNNNTGC 4 cut(s) 1194, 1217, 1228, 1251
BciT130I CCWGG 2 cut(s) 791, 966
BciVI GTATCC 1 cut(s) 872
BclI TGATCA 2 cut(s) 426, 799
BcoDI GTCTC 2 cut(s) 9, 902
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 4 cut(s) 231, 314, 914, 1187
BfmI CTRYAG 2 cut(s) 56, 624
BfuI GTATCC 1 cut(s) 872
BglII AGATCT 1 cut(s) 758
BisI GCNGC 1 cut(s) 869
BlsI GCNGC 1 cut(s) 870
BmcAI AGTACT 1 cut(s) 903
Bme1390I CCNGG 2 cut(s) 791, 966
Bme18I GGWCC 3 cut(s) 227, 638, 787
BmgT120I GGNCC 4 cut(s) 137, 227, 638, 787
BmrFI CCNGG 2 cut(s) 791, 966
BmsI GCATC 1 cut(s) 196
BmtI GCTAGC 1 cut(s) 317
BpiI GAAGAC 3 cut(s) 9, 500, 527
BplI GAGNNNNNCTC 4 cut(s) 653, 685, 875, 907
BpmI CTGGAG 2 cut(s) 926, 1056
Bpu10I CCTNAGC 1 cut(s) 783
BpuEI CTTGAG 1 cut(s) 877
BsaJI CCNNGG 1 cut(s) 964
BsaXI ACNNNNNCTCC 4 cut(s) 716, 746, 1245, 1275
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 971
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 2 cut(s) 899, 909
Bse3DI GCAATG 2 cut(s) 348, 876
BseBI CCWGG 2 cut(s) 791, 966
BseDI CCNNGG 1 cut(s) 964
BseLI CCNNNNNNNGG 2 cut(s) 545, 971
BseMI GCAATG 2 cut(s) 348, 876
BseMII CTCAG 2 cut(s) 38, 246
BseNI ACTGG 2 cut(s) 899, 909
BseRI GAGGAG 2 cut(s) 55, 130
BseSI GKGCMC 1 cut(s) 965
BseXI GCAGC 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 3 cut(s) 138, 383, 898
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 384, 585
BslI CCNNNNNNNGG 2 cut(s) 545, 971
BsmAI GTCTC 2 cut(s) 9, 902
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 3 cut(s) 138, 383, 898
Bsp1286I GDGCHC 2 cut(s) 65, 965
Bsp143I GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
BspACI CCGC 4 cut(s) 335, 617, 866, 979
BspANI GGCC 3 cut(s) 138, 383, 898
BspCNI CTCAG 2 cut(s) 37, 247
BspFNI CGCG 1 cut(s) 335
BspMAI CTGCAG 1 cut(s) 60
BspOI GCTAGC 1 cut(s) 317
BspPI GGATC 1 cut(s) 1191
BspQI GCTCTTC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 979
BsrDI GCAATG 2 cut(s) 348, 876
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 2 cut(s) 899, 909
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 964
BssMI GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
BssSI CACGAG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 791, 966
Bst4CI ACNGT 3 cut(s) 499, 817, 1061
Bst6I CTCTTC 3 cut(s) 35, 53, 73
BstC8I GCNNGC 3 cut(s) 136, 201, 315
BstDEI CTNAG 3 cut(s) 24, 255, 783
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 7 cut(s) 408, 429, 761, 802, 1075, 1176, 1186
BstMAI GTCTC 2 cut(s) 9, 902
BstMBI GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 2 cut(s) 791, 966
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 789, 964
BstSFI CTRYAG 2 cut(s) 56, 624
BstSLI GKGCMC 1 cut(s) 965
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 855
BstV2I GAAGAC 3 cut(s) 9, 500, 527
BstX2I RGATCY 2 cut(s) 758, 1183
BstYI RGATCY 2 cut(s) 758, 1183
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 3 cut(s) 138, 383, 898
BtsI GCAGTG 2 cut(s) 399, 453
BtsIMutI CAGTG 3 cut(s) 399, 453, 1066
Cac8I GCNNGC 3 cut(s) 136, 201, 315
CaiI CAGNNNCTG 2 cut(s) 1022, 1196
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 137, 227, 638, 787
Csp6I GTAC 2 cut(s) 393, 902
CspCI CAANNNNNGTGG 2 cut(s) 93, 128
CviAII CATG 1 cut(s) 727
CviQI GTAC 2 cut(s) 393, 902
DdeI CTNAG 3 cut(s) 24, 255, 783
DpnI GATC 7 cut(s) 407, 428, 760, 801, 1074, 1175, 1185
DpnII GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 3 cut(s) 35, 53, 73
EarI CTCTTC 3 cut(s) 35, 53, 73
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 65
Eco32I GATATC 2 cut(s) 150, 889
Eco47I GGWCC 3 cut(s) 227, 638, 787
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 2 cut(s) 227, 787
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 789, 964
EcoRV GATATC 2 cut(s) 150, 889
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 1 cut(s) 730
FaiI YATR 8 cut(s) 85, 308, 602, 626, 728, 1013, 1124, 1280
FatI CATG 1 cut(s) 726
FauI CCCGC 2 cut(s) 328, 873
FbaI TGATCA 2 cut(s) 426, 799
Fnu4HI GCNGC 1 cut(s) 869
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 869
FspBI CTAG 4 cut(s) 231, 314, 914, 1187
GluI GCNGC 1 cut(s) 869
GsuI CTGGAG 2 cut(s) 926, 1056
HaeIII GGCC 3 cut(s) 138, 383, 898
HapII CCGG 2 cut(s) 384, 585
Hin1II CATG 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 4 cut(s) 118, 257, 662, 1153
HpaII CCGG 2 cut(s) 384, 585
HphI GGTGA 4 cut(s) 139, 398, 931, 1108
Hpy166II GTNNAC 1 cut(s) 938
Hpy188I TCNGA 4 cut(s) 256, 262, 1072, 1228
Hpy188III TCNNGA 5 cut(s) 641, 752, 1035, 1177, 1215
Hpy8I GTNNAC 1 cut(s) 938
HpyAV CCTTC 4 cut(s) 543, 695, 1146, 1174
HpyCH4III ACNGT 3 cut(s) 499, 817, 1061
HpyCH4IV ACGT 1 cut(s) 238
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 3 cut(s) 24, 255, 783
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 1 cut(s) 730
Ksp22I TGATCA 2 cut(s) 426, 799
Kzo9I GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 2 cut(s) 68, 338
Lsp1109I GCAGC 1 cut(s) 855
LweI GCATC 1 cut(s) 196
MaeI CTAG 4 cut(s) 231, 314, 914, 1187
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 4 cut(s) 289, 722, 793, 1096
MalI GATC 7 cut(s) 407, 428, 760, 801, 1074, 1175, 1185
MbiI CCGCTC 1 cut(s) 979
MboI GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
MfeI CAATTG 4 cut(s) 294, 324, 657, 854
MflI RGATCY 2 cut(s) 758, 1183
MhlI GDGCHC 2 cut(s) 65, 965
MlyI GAGTC 3 cut(s) 127, 266, 671
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 2 cut(s) 384, 585
MspR9I CCNGG 2 cut(s) 791, 966
MunI CAATTG 4 cut(s) 294, 324, 657, 854
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 2 cut(s) 791, 966
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 458
NdeII GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 1 cut(s) 730
NmuCI GTSAC 1 cut(s) 1096
NspI RCATGY 1 cut(s) 730
PciI ACATGT 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 35
PctI GAATGC 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 1 cut(s) 1153
PkrI GCNGC 1 cut(s) 870
PleI GAGTC 3 cut(s) 126, 265, 670
PpsI GAGTC 3 cut(s) 126, 265, 670
PpuMI RGGWCCY 2 cut(s) 227, 787
PscI ACATGT 1 cut(s) 726
PshBI ATTAAT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 2 cut(s) 227, 787
Psp6I CCWGG 2 cut(s) 789, 964
PspGI CCWGG 2 cut(s) 789, 964
PspPI GGNCC 4 cut(s) 137, 227, 638, 787
PspPPI RGGWCCY 2 cut(s) 227, 787
PsrI GAACNNNNNNTAC 2 cut(s) 282, 314
PstI CTGCAG 1 cut(s) 60
PstNI CAGNNNCTG 2 cut(s) 1022, 1196
PsuI RGATCY 2 cut(s) 758, 1183
RsaI GTAC 2 cut(s) 394, 903
RsaNI GTAC 2 cut(s) 393, 902
SacI GAGCTC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
SatI GCNGC 1 cut(s) 869
Sau3AI GATC 7 cut(s) 405, 426, 758, 799, 1072, 1173, 1183
Sau96I GGNCC 4 cut(s) 137, 227, 638, 787
ScaI AGTACT 1 cut(s) 903
SchI GAGTC 3 cut(s) 127, 266, 671
ScrFI CCNGG 2 cut(s) 791, 966
SduI GDGCHC 2 cut(s) 65, 965
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 2 cut(s) 56, 624
SinI GGWCC 3 cut(s) 227, 638, 787
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
SpeI ACTAGT 1 cut(s) 913
SsiI CCGC 4 cut(s) 335, 617, 866, 979
SspI AATATT 1 cut(s) 1167
SspMI CTAG 4 cut(s) 231, 314, 914, 1187
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 789, 964
TaaI ACNGT 3 cut(s) 499, 817, 1061
TaiI ACGT 1 cut(s) 241
TaqI TCGA 1 cut(s) 1214
TatI WGTACW 1 cut(s) 901
TfiI GAWTC 1 cut(s) 1153
Tru1I TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
Tru9I TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
TscAI CASTG 3 cut(s) 406, 453, 1066
TseFI GTSAC 1 cut(s) 1096
TseI GCWGC 1 cut(s) 868
Tsp45I GTSAC 1 cut(s) 1096
TspDTI ATGAA 4 cut(s) 386, 589, 609, 730
TspRI CASTG 3 cut(s) 406, 453, 1066
VpaK11BI GGWCC 3 cut(s) 227, 638, 787
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 1 cut(s) 596
XceI RCATGY 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 4 cut(s) 231, 314, 914, 1187
ZrmI AGTACT 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.