RchiOBHm_Chr5g0020251
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005115
Physical Location & Seq
Forward (+)
14489658 .. 14494191
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30035

Sequence Viewer

Length: 1758 bp
ATGTCTCTCTCTCTGTCTTCCTCTCAGCTCTTCCTTTACACACCTCTCTTCTCTCTGCAGAGCTCCTCCCAGAAGCGAAGAGTATGTGGGTTTTGGAAAAAGCCACAGGCTTTGAGGAGTCTTGTGGTGAGAGCAGGCCCAAAGAAGATATCTTTTGGTAAAGAATGCAGAGAGGGATTGCAAATTGGGATAGATAAGCTTGCTGATGCTGTTTCTCTTACATTAGGACCTAGAGGACGTAATGTTGTTCTTTCTGAGTCTGAAAAAGTGAGAGTGATTAATGATGGTGTAACAATTGTTCAGTCCATAGAGCTAGCAGATACAATTGAGAACGCGGGAGCAATGCTAATCCAAGAGGTTGCAAGTAAAATGAATAATTTGGCCGGTGATGGTACTACCACTGCGATCATTTTGACACGAGAAATGATCAAAGGTGGATTATTGGCAGTGGCTTTTGGGGCTAACCCAATTTCTTTGAAGAAAGGAATGGAGAAGACTGTTAGTGAATTGGTCAAGGTGTTAAAGGAGAAAAGTCTTCCCGTAAAAGGAAGGAATGATATTAAAGCTGTGGCTTCAATTTCTGCCGGAAATGATGAATTCATAGGAAACTTGATTGCGGAAGCTATAGAAAAGATTGGTCCTGATGGAGTAATCTCAATTGAGTCGTCCTCATCATTTGAAACATCTGTTTTAGTTGAAGAAGGACTAAAGTTTGAAAAGGGTTACATGTCCTCCCACTTCATTACTAATCAAGAAAGATCTATTGTGGAGTTTGACAAGGCTAAGGTCCTGGTAACTGATCAAAAGATTTCAACTGTTAAAGAAATTGTTCCTTTGCTGGAAAAGATAACCCAATTGAGTATCCCGCTGCTAATCATTGCAGAGGATATCTCAAGGCCAGTACTGGAGACACTAGTGGTGAATAAAATGCAGGGTTTACTTAATGTTGCTGTTGTTAAGTGCCCTGGATTTGGAGAGCGGAAGAAAGCTCTTTTGCAAGATATTGCACTTATGACAGGTGCTGATTTTCTCTCTGGAGATTTTGGTTTTACCCTTGACAGTGCAACATCAGATCAACTTGGAATTGCAAGAAAGGTGACAATAACAAGTAATTCAACAACCATAGTTGCTGACCCTTCTACCAAAGCAGAGATTCAGGCAAGAATATTGCAGATCAAGAAGGATCTAGCAGAAACTGAAAATGCAAACCTGTCGAGAAAGCTCTCCGAAAGAATTGCTAAACTCTGTGGTGGAGTTGCCGTAATTAAGGTAGGAGCACATACTGAGGTGGAACTTGAAGATCGCAAACTCAGAATTGAGGATGCAAAGAATGCCACATTTGCTGCTATGGAGGAAGGGGTAGTGCCTGGTGGTGGTGCCACTTATGTCCATCTGTCAGAATTGATTCCTGTCATAAAGAATTCTATGGAAGATTCTGATGAGCAGATTGGTGCCGACATTATAGCGAAGGCTCTCCTTGCTCCTGCAAAATCAATTGCAACTAATGCTGGGGATGAGGGAGAAGTTGTTGTGGAGAAGACAAAAAATTGTGATTGGAGAATCGGGTACAATGCCATGACTGGCAAGTATGAAGATCTTCCAGATGCAGGAGTTCTAGATCCTTGTCGGGTTTCAAGATGTGCCCTTCAAATTGCAGTCTCCATTGCTGGGGTGGTTCTTACAACTCAAGCCGTATTGGTGGAGAAAACAAAGACGAAAAAGCCAGCTATTCCTTCTGTTCCGGGAATAAGTCCTTAG

Protein Analysis

585

Amino Acids

62.6

Weight (kDa)

6.47

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 67 - 569 3.5e-75 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1376, 1451
AccBSI CCGCTC 1 cut(s) 979
AccII CGCG 1 cut(s) 335
AciI CCGC 4 cut(s) 335, 617, 866, 979
AclWI GGATC 2 cut(s) 1191, 1613
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 2 cut(s) 596, 1420
AfaI GTAC 3 cut(s) 394, 903, 1568
AfiI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
AflIII ACRYGT 1 cut(s) 726
AhlI ACTAGT 1 cut(s) 913
AjnI CCWGG 3 cut(s) 789, 964, 1366
AluBI AGCT 9 cut(s) 28, 63, 199, 313, 566, 623, 989, 1222, 1727
AluI AGCT 9 cut(s) 28, 63, 199, 313, 566, 623, 989, 1222, 1727
Alw21I GWGCWC 2 cut(s) 65, 1279
Alw26I GTCTC 3 cut(s) 9, 902, 1663
AlwI GGATC 2 cut(s) 1191, 1613
AlwNI CAGNNNCTG 2 cut(s) 1022, 1196
AoxI GGCC 3 cut(s) 136, 381, 896
ApeKI GCWGC 2 cut(s) 868, 1343
ApoI RAATTY 2 cut(s) 596, 1420
AseI ATTAAT 1 cut(s) 279
Asp700I GAANNNNTTC 3 cut(s) 828, 1404, 1596
AspS9I GGNCC 4 cut(s) 137, 227, 638, 787
AsuC2I CCSGG 1 cut(s) 1743
AsuHPI GGTGA 4 cut(s) 139, 398, 931, 1108
AsuNHI GCTAGC 1 cut(s) 313
AvaII GGWCC 3 cut(s) 227, 638, 787
BaeGI GKGCMC 2 cut(s) 965, 1645
BanI GGYRCC 2 cut(s) 1376, 1451
BanII GRGCYC 1 cut(s) 65
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 4 cut(s) 9, 500, 527, 1544
Bbv12I GWGCWC 2 cut(s) 65, 1279
BbvI GCAGC 2 cut(s) 855, 1330
BccI CCATC 4 cut(s) 278, 383, 638, 1398
BceAI ACGGC 2 cut(s) 1244, 1676
BcgI CGANNNNNNTGC 4 cut(s) 1194, 1217, 1228, 1251
BciT130I CCWGG 3 cut(s) 791, 966, 1368
BciVI GTATCC 1 cut(s) 872
BclI TGATCA 2 cut(s) 426, 799
BcnI CCSGG 1 cut(s) 1743
BcoDI GTCTC 3 cut(s) 9, 902, 1663
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 5 cut(s) 231, 314, 914, 1187, 1616
BfmI CTRYAG 2 cut(s) 56, 624
BfuI GTATCC 1 cut(s) 872
BglII AGATCT 2 cut(s) 758, 1594
BisI GCNGC 2 cut(s) 869, 1344
BlsI GCNGC 2 cut(s) 870, 1345
BmcAI AGTACT 1 cut(s) 903
Bme1390I CCNGG 4 cut(s) 791, 966, 1368, 1743
Bme18I GGWCC 3 cut(s) 227, 638, 787
BmgT120I GGNCC 4 cut(s) 137, 227, 638, 787
BmiI GGNNCC 2 cut(s) 1378, 1453
BmrFI CCNGG 4 cut(s) 791, 966, 1368, 1743
BmsI GCATC 3 cut(s) 196, 1312, 1594
BmtI GCTAGC 1 cut(s) 317
BpiI GAAGAC 4 cut(s) 9, 500, 527, 1544
BplI GAGNNNNNCTC 4 cut(s) 653, 685, 875, 907
BpmI CTGGAG 2 cut(s) 926, 1056
Bpu10I CCTNAGC 1 cut(s) 783
BpuEI CTTGAG 2 cut(s) 877, 1671
BpuMI CCSGG 1 cut(s) 1743
BsaJI CCNNGG 1 cut(s) 964
BsaXI ACNNNNNCTCC 6 cut(s) 716, 746, 1245, 1275, 1512, 1542
Bsc4I CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 3 cut(s) 899, 909, 1585
Bse3DI GCAATG 3 cut(s) 348, 876, 1662
BseBI CCWGG 3 cut(s) 791, 966, 1368
BseDI CCNNGG 1 cut(s) 964
BseGI GGATG 2 cut(s) 1327, 1519
BseLI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
BseMI GCAATG 3 cut(s) 348, 876, 1662
BseMII CTCAG 4 cut(s) 38, 246, 1275, 1324
BseNI ACTGG 3 cut(s) 899, 909, 1585
BseRI GAGGAG 2 cut(s) 55, 130
BseSI GKGCMC 2 cut(s) 965, 1645
BseXI GCAGC 2 cut(s) 855, 1330
BseYI CCCAGC 2 cut(s) 1508, 1667
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 3 cut(s) 138, 383, 898
BshNI GGYRCC 2 cut(s) 1376, 1451
BsiHKAI GWGCWC 2 cut(s) 65, 1279
BsiSI CCGG 3 cut(s) 384, 585, 1742
BslI CCNNNNNNNGG 5 cut(s) 545, 971, 1373, 1607, 1668
BsmAI GTCTC 3 cut(s) 9, 902, 1663
BsmI GAATGC 2 cut(s) 170, 1336
BsnI GGCC 3 cut(s) 138, 383, 898
Bsp1286I GDGCHC 4 cut(s) 65, 965, 1279, 1645
BspACI CCGC 4 cut(s) 335, 617, 866, 979
BspANI GGCC 3 cut(s) 138, 383, 898
BspCNI CTCAG 4 cut(s) 37, 247, 1276, 1323
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 2 cut(s) 1378, 1453
BspMAI CTGCAG 1 cut(s) 60
BspOI GCTAGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 1191, 1613
BspQI GCTCTTC 1 cut(s) 35
BspT107I GGYRCC 2 cut(s) 1376, 1451
BsrBI CCGCTC 1 cut(s) 979
BsrDI GCAATG 3 cut(s) 348, 876, 1662
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 3 cut(s) 899, 909, 1585
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 964
BssSI CACGAG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 791, 966, 1368
Bst4CI ACNGT 3 cut(s) 499, 817, 1061
Bst6I CTCTTC 3 cut(s) 35, 53, 73
BstAPI GCANNNNNTGC 2 cut(s) 1331, 1505
BstC8I GCNNGC 4 cut(s) 136, 201, 315, 1725
BstDEI CTNAG 6 cut(s) 24, 255, 783, 1284, 1310, 1755
BstF5I GGATG 2 cut(s) 1327, 1519
BstFNI CGCG 1 cut(s) 335
BstMAI GTCTC 3 cut(s) 9, 902, 1663
BstMWI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
BstNI CCWGG 3 cut(s) 791, 966, 1368
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 4 cut(s) 789, 964, 1366, 1741
BstSFI CTRYAG 2 cut(s) 56, 624
BstSLI GKGCMC 2 cut(s) 965, 1645
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 2 cut(s) 855, 1330
BstV2I GAAGAC 4 cut(s) 9, 500, 527, 1544
BstX2I RGATCY 4 cut(s) 758, 1183, 1594, 1618
BstYI RGATCY 4 cut(s) 758, 1183, 1594, 1618
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 3 cut(s) 138, 383, 898
BtsCI GGATG 2 cut(s) 1327, 1519
BtsI GCAGTG 2 cut(s) 399, 453
BtsIMutI CAGTG 3 cut(s) 399, 453, 1066
Cac8I GCNNGC 4 cut(s) 136, 201, 315, 1725
CaiI CAGNNNCTG 2 cut(s) 1022, 1196
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 137, 227, 638, 787
Csp6I GTAC 3 cut(s) 393, 902, 1567
CspCI CAANNNNNGTGG 2 cut(s) 93, 128
CviAII CATG 2 cut(s) 727, 1576
CviQI GTAC 3 cut(s) 393, 902, 1567
DdeI CTNAG 6 cut(s) 24, 255, 783, 1284, 1310, 1755
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 3 cut(s) 35, 53, 73
EarI CTCTTC 3 cut(s) 35, 53, 73
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 65
Eco32I GATATC 2 cut(s) 150, 889
Eco47I GGWCC 3 cut(s) 227, 638, 787
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 2 cut(s) 227, 787
EcoRI GAATTC 2 cut(s) 596, 1420
EcoRII CCWGG 3 cut(s) 789, 964, 1366
EcoRV GATATC 2 cut(s) 150, 889
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 2 cut(s) 730, 1579
FatI CATG 2 cut(s) 726, 1575
FauI CCCGC 2 cut(s) 328, 873
FbaI TGATCA 2 cut(s) 426, 799
Fnu4HI GCNGC 2 cut(s) 869, 1344
FokI GGATG 2 cut(s) 1334, 1526
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 869, 1344
FspBI CTAG 5 cut(s) 231, 314, 914, 1187, 1616
GluI GCNGC 2 cut(s) 869, 1344
GsaI CCCAGC 2 cut(s) 1512, 1671
GsuI CTGGAG 2 cut(s) 926, 1056
HaeIII GGCC 3 cut(s) 138, 383, 898
HapII CCGG 3 cut(s) 384, 585, 1742
Hin1II CATG 2 cut(s) 730, 1579
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 7 cut(s) 118, 257, 662, 1153, 1405, 1433, 1560
HpaII CCGG 3 cut(s) 384, 585, 1742
HphI GGTGA 4 cut(s) 139, 398, 931, 1108
Hpy166II GTNNAC 1 cut(s) 938
Hpy188I TCNGA 7 cut(s) 256, 262, 1072, 1228, 1313, 1399, 1438
Hpy188III TCNNGA 8 cut(s) 641, 752, 1035, 1177, 1215, 1601, 1616, 1635
Hpy8I GTNNAC 1 cut(s) 938
HpyAV CCTTC 8 cut(s) 543, 695, 1146, 1174, 1349, 1462, 1655, 1743
HpyCH4III ACNGT 3 cut(s) 499, 817, 1061
HpyCH4IV ACGT 1 cut(s) 238
HpyF10VI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
HpyF3I CTNAG 6 cut(s) 24, 255, 783, 1284, 1310, 1755
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 2 cut(s) 730, 1579
Ksp22I TGATCA 2 cut(s) 426, 799
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 4 cut(s) 68, 338, 1274, 1486
Lsp1109I GCAGC 2 cut(s) 855, 1330
LweI GCATC 3 cut(s) 196, 1312, 1594
MaeI CTAG 5 cut(s) 231, 314, 914, 1187, 1616
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 4 cut(s) 289, 722, 793, 1096
MbiI CCGCTC 1 cut(s) 979
MfeI CAATTG 5 cut(s) 294, 324, 657, 854, 1494
MflI RGATCY 4 cut(s) 758, 1183, 1594, 1618
MhlI GDGCHC 4 cut(s) 65, 965, 1279, 1645
MlyI GAGTC 3 cut(s) 127, 266, 671
MroXI GAANNNNTTC 3 cut(s) 828, 1404, 1596
MseI TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 3 cut(s) 384, 585, 1742
MspR9I CCNGG 4 cut(s) 791, 966, 1368, 1743
MunI CAATTG 5 cut(s) 294, 324, 657, 854, 1494
Mva1269I GAATGC 2 cut(s) 170, 1336
MvaI CCWGG 3 cut(s) 791, 966, 1368
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 458, 1331, 1340, 1478, 1505
NciI CCSGG 1 cut(s) 1743
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 2 cut(s) 730, 1579
NlaIV GGNNCC 2 cut(s) 1378, 1453
NmuCI GTSAC 1 cut(s) 1096
NspI RCATGY 1 cut(s) 730
PciI ACATGT 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 35
PctI GAATGC 2 cut(s) 170, 1336
PdmI GAANNNNTTC 3 cut(s) 828, 1404, 1596
PfeI GAWTC 4 cut(s) 1153, 1405, 1433, 1560
PfoI TCCNGGA 1 cut(s) 1741
PkrI GCNGC 2 cut(s) 870, 1345
PleI GAGTC 3 cut(s) 126, 265, 670
PpsI GAGTC 3 cut(s) 126, 265, 670
PpuMI RGGWCCY 2 cut(s) 227, 787
PscI ACATGT 1 cut(s) 726
PshBI ATTAAT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 2 cut(s) 227, 787
Psp6I CCWGG 3 cut(s) 789, 964, 1366
PspFI CCCAGC 2 cut(s) 1508, 1667
PspGI CCWGG 3 cut(s) 789, 964, 1366
PspN4I GGNNCC 2 cut(s) 1378, 1453
PspPI GGNCC 4 cut(s) 137, 227, 638, 787
PspPPI RGGWCCY 2 cut(s) 227, 787
PsrI GAACNNNNNNTAC 2 cut(s) 282, 314
PstI CTGCAG 1 cut(s) 60
PstNI CAGNNNCTG 2 cut(s) 1022, 1196
PsuI RGATCY 4 cut(s) 758, 1183, 1594, 1618
RsaI GTAC 3 cut(s) 394, 903, 1568
RsaNI GTAC 3 cut(s) 393, 902, 1567
SacI GAGCTC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
SatI GCNGC 2 cut(s) 869, 1344
Sau96I GGNCC 4 cut(s) 137, 227, 638, 787
ScaI AGTACT 1 cut(s) 903
SchI GAGTC 3 cut(s) 127, 266, 671
ScrFI CCNGG 4 cut(s) 791, 966, 1368, 1743
SduI GDGCHC 4 cut(s) 65, 965, 1279, 1645
SfaNI GCATC 3 cut(s) 196, 1312, 1594
SfcI CTRYAG 2 cut(s) 56, 624
SinI GGWCC 3 cut(s) 227, 638, 787
SmlI CTYRAG 2 cut(s) 892, 1686
SmoI CTYRAG 2 cut(s) 892, 1686
SpeI ACTAGT 1 cut(s) 913
SsiI CCGC 4 cut(s) 335, 617, 866, 979
SspI AATATT 1 cut(s) 1167
SspMI CTAG 5 cut(s) 231, 314, 914, 1187, 1616
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 4 cut(s) 789, 964, 1366, 1741
TaaI ACNGT 3 cut(s) 499, 817, 1061
TaiI ACGT 1 cut(s) 241
TaqI TCGA 1 cut(s) 1214
TatI WGTACW 1 cut(s) 901
TfiI GAWTC 4 cut(s) 1153, 1405, 1433, 1560
Tru1I TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
Tru9I TTAA 7 cut(s) 279, 521, 561, 819, 942, 957, 1266
TscAI CASTG 3 cut(s) 406, 453, 1066
TseFI GTSAC 1 cut(s) 1096
TseI GCWGC 2 cut(s) 868, 1343
Tsp45I GTSAC 1 cut(s) 1096
TspDTI ATGAA 5 cut(s) 386, 589, 609, 730, 1605
TspRI CASTG 3 cut(s) 406, 453, 1066
VpaK11BI GGWCC 3 cut(s) 227, 638, 787
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 2 cut(s) 596, 1420
XbaI TCTAGA 1 cut(s) 1615
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XmnI GAANNNNTTC 3 cut(s) 828, 1404, 1596
XspI CTAG 5 cut(s) 231, 314, 914, 1187, 1616
ZrmI AGTACT 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.