Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005115

Physical Location & Seq

Genomic Coordinates Forward (+)

29445844 .. 29450535

Gene Structure 4692 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1228200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGTCCATCTCCTTCCCCTCCTCCTCCTTTCAACTCTTCTTTTATAAACCTTTCTTCTCTCAGCAGAGCAATTTTCAGAAGCAACAAGTAGCTGGGTTTTGGAGAAAGCCAGAGTCTTTGAGGAGGTTGGTGGTCAAAGCAGGCGCAAAAAAGATATCTTTTGGTACAGAATGCAGGGATGCCTTACAAACTGGGATAGATAAACTTGCTGATGCTGTTTCCCTTACCTTAGGACCTAAAGGACGTAATGTTGTTCTTTCTGAGTCTGAAAAACTGAAAGTGATAAATGATGGTGTAACAATTGTTCAGTCCATAGAGCTATCAGATACAATTGAGAACGTGGGAGCGATGCTAATCCAAGAGGTTGCAAAGAAAATGAATAATTTGGCAGGTGATGGTACCACCACTGCAGTCATTTTGACAAGAGAAATTATCAGAGGTGGACTTTTGGCAGTAGCTTTTGGAGCTAACCCAATTTCTTTGAAGAAAGGAATGGATAAGACTGTAAATGAACTGGTCAAGATCTTGAAGGAGAAGAGTCTTCCAGTAAAAGGAAGGAAAGACATTAAAGCTGTAGCTTCAATTTCTGCTGGAAATGACAAGTTCATCGGTAATTTGATTGCTGAAGCTATAGAAAAGATTGGACCTGATGGAGTAATCTCAATTGAGTCATCCTCATCATTTGAAACGTCTGTTTTAGTTGAAGAAGGGCTGAAGTTTGACAAGGGATACATGTCCTCCCACTTCATTACGAACCAGGAAAAATCTCTTGTGGAGTTTGACAAAGCTAAGGTCCTAGTAACTGATCACAAGATCTCAACTGTTAGAGAAATTGTTCCTTTATTGGAAAAGACAACCCAACTGAGTATCCCGCTGCTAATCATTGCAGAGGATATCTCAAAGCAAGTACTGGAAACACTAGTGGTGAACAAAAAGCAGGGTTTACTTAAAGTCGCTGTTGTTAAATGTCCTGGATTTGCAGAGGGGAAAAAAGCTATTCTGCAAGATATTGCACTTATGACAGGTGCTGATTTTCTCTCTGGAGATTTTGGTTTGACCCTTGAGAGTGCAACATCAGATCAACTTGGAATTGCAAGAAAAGTGAAAATAACAAGTAATTCAACAACGATAGTTGCAGACCCTTCTACTAAAGCTGAGATTCAGGCAAGAATATTACAGATCAAGAAGGATCTGGCAGAAACAGAAAATGCATACCTGTCGAGAAAGCTCTCTGAAAGAATTGCCAAACTTTGCGGTGGAGTTGCTGTAATTAAGGTAGGAGCACATACTGAGGTAGAACTTGAAGATCGCAAACTCAGAATTGAAGATGCAAAGAACTCTACATTTGCTGCTATGGAGGAAGGAGTGGTGCCTGGTGGTGGTGCCACATATGTACATCTCTCAGAATTTATTCCTGCCATAAAGAATTCTATGGAAGATTTAGATGAGCAGATTGGTGCTGACCTTGTAGCAAAGGCTCTACTTGCTCCTGCAAAAGCAATTGCAACTAATGCAGGGGATGATGGAGAAGTTGTTGTGGAGAAGACTAAAAGTTCTGATTGGAGAGTTGGGTACAATGCAATGACAGGAAACTATGAAGATCTCCCAGATGCGGGAGTTATAGATCCTTGTAGGGTTTCAAGATGTGCCCTTCAAATTGCAGCCTCCATTGCCGGAGTGGTTCTTACAACTCAAGCTGTATTGGTGGAGAAAACAAAGAAGAAAAAGCCAGCTGTTCCTTTTGTTCCGGGAATAACTCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006457 GO:0006458 GO:0006996 GO:0007005 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044183 GO:0044424 GO:0044444 GO:0044464 GO:0051082 GO:0061077 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

589

Amino Acids

63.17

Weight (kDa)

8.3

Isoelectric Point (pI)

33.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	69 - 571	1.2e-74	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18820 AT5G18820 AT5G18820
fragaria_vesca	FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390 FvH4_3g12390
malus_domestica	MD08G1228200.v1.1 MD15G1422100.v1.1
prunus_persica	Prupe.4G112900_v2.0.a1 Prupe.4G112900_v2.0.a1
pyrus_communis	pycom15g37410
rosa_chinensis	RchiOBHm_Chr5g0020251
rosa_laevigata	RLG00000032512
rosa_multiflora	Rmu_sc0002087.1_g000009
rosa_roxburghii	Rroxscaffold_1G00057960
rosa_rugosa	Rorug05G0057800 Rorug05G0057900
rosa_samantha	Rh5AG148400 Rh5BG147900 Rh5CG159300 Rh5DG147000
rosa_wichuraiana	Rw5G013130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	45
AarI	CACCTGC	1 cut(s)	380
Acc36I	ACCTGC	1 cut(s)	380
Acc65I	GGTACC	1 cut(s)	398
AccB1I	GGYRCC	3 cut(s)	398, 1369, 1382
AciI	CCGC	3 cut(s)	872, 1254, 1613
AclWI	GGATC	2 cut(s)	1197, 1619
AcsI	RAATTY	2 cut(s)	1406, 1426
AcuI	CTGAAG	2 cut(s)	645, 734
AfaI	GTAC	5 cut(s)	166, 400, 909, 1395, 1574
AfiI	CCNNNNNNNGG	4 cut(s)	551, 1379, 1612, 1613
AflIII	ACRYGT	1 cut(s)	732
AhlI	ACTAGT	1 cut(s)	919
AjnI	CCWGG	3 cut(s)	756, 970, 1372
Alw21I	GWGCWC	1 cut(s)	1285
AlwI	GGATC	2 cut(s)	1197, 1619
AlwNI	CAGNNNCTG	1 cut(s)	1028
ApeKI	GCWGC	3 cut(s)	874, 1349, 1661
ApoI	RAATTY	2 cut(s)	1406, 1426
ArsI	GACNNNNNNTTYG	2 cut(s)	777, 809
Asp700I	GAANNNNTTC	2 cut(s)	834, 1410
Asp718I	GGTACC	1 cut(s)	398
AspLEI	GCGC	1 cut(s)	146
AspS9I	GGNCC	3 cut(s)	233, 644, 793
AsuC2I	CCSGG	1 cut(s)	1749
AsuHPI	GGTGA	2 cut(s)	404, 937
AvaII	GGWCC	3 cut(s)	233, 644, 793
AxyI	CCTNAGG	1 cut(s)	229
BaeGI	GKGCMC	1 cut(s)	1651
BanI	GGYRCC	3 cut(s)	398, 1369, 1382
BbsI	GAAGAC	2 cut(s)	533, 1550
Bbv12I	GWGCWC	1 cut(s)	1285
BbvI	GCAGC	3 cut(s)	861, 1336, 1673
BccI	CCATC	5 cut(s)	14, 284, 389, 644, 1517
BcgI	CGANNNNNNTGC	2 cut(s)	1200, 1234
BciT130I	CCWGG	3 cut(s)	758, 972, 1374
BciVI	GTATCC	2 cut(s)	722, 878
BclI	TGATCA	1 cut(s)	805
BcnI	CCSGG	1 cut(s)	1749
BcuI	ACTAGT	1 cut(s)	919
BfaI	CTAG	2 cut(s)	797, 920
BfmI	CTRYAG	3 cut(s)	408, 573, 630
BfuAI	ACCTGC	1 cut(s)	380
BfuI	GTATCC	2 cut(s)	722, 878
BglII	AGATCT	3 cut(s)	522, 813, 1600
BisI	GCNGC	3 cut(s)	875, 1350, 1662
BlsI	GCNGC	3 cut(s)	876, 1351, 1663
BmcAI	AGTACT	1 cut(s)	909
Bme1390I	CCNGG	4 cut(s)	758, 972, 1374, 1749
Bme18I	GGWCC	3 cut(s)	233, 644, 793
BmgT120I	GGNCC	3 cut(s)	233, 644, 793
BmiI	GGNNCC	3 cut(s)	400, 1371, 1384
BmrFI	CCNGG	4 cut(s)	758, 972, 1374, 1749
BmrI	ACTGGG	1 cut(s)	201
BmsI	GCATC	5 cut(s)	169, 202, 339, 1318, 1600
BmuI	ACTGGG	1 cut(s)	201
BpiI	GAAGAC	2 cut(s)	533, 1550
BplI	GAGNNNNNCTC	4 cut(s)	659, 691, 881, 913
BpmI	CTGGAG	1 cut(s)	1062
Bpu10I	CCTNAGC	1 cut(s)	789
BpuEI	CTTGAG	2 cut(s)	1082, 1677
BpuMI	CCSGG	1 cut(s)	1749
BsaBI	GATNNNNATC	1 cut(s)	353
BsaXI	ACNNNNNCTCC	6 cut(s)	722, 752, 1251, 1281, 1518, 1548
Bsc4I	CCNNNNNNNGG	4 cut(s)	551, 1379, 1612, 1613
Bse1I	ACTGG	4 cut(s)	196, 519, 545, 915
Bse21I	CCTNAGG	1 cut(s)	229
Bse3DI	GCAATG	3 cut(s)	882, 1587, 1668
Bse8I	GATNNNNATC	1 cut(s)	353
BseBI	CCWGG	3 cut(s)	758, 972, 1374
BseGI	GGATG	3 cut(s)	184, 671, 1525
BseJI	GATNNNNATC	1 cut(s)	353
BseLI	CCNNNNNNNGG	4 cut(s)	551, 1379, 1612, 1613
BseMI	GCAATG	3 cut(s)	882, 1587, 1668
BseMII	CTCAG	7 cut(s)	74, 252, 854, 1146, 1281, 1330, 1416
BseNI	ACTGG	4 cut(s)	196, 519, 545, 915
BseRI	GAGGAG	4 cut(s)	10, 13, 136, 1749
BseSI	GKGCMC	1 cut(s)	1651
BseXI	GCAGC	3 cut(s)	861, 1336, 1673
BseYI	CCCAGC	1 cut(s)	92
BshNI	GGYRCC	3 cut(s)	398, 1369, 1382
BsiHKAI	GWGCWC	1 cut(s)	1285
BsiSI	CCGG	2 cut(s)	1674, 1748
BslI	CCNNNNNNNGG	4 cut(s)	551, 1379, 1612, 1613
BsmI	GAATGC	1 cut(s)	176
Bsp1286I	GDGCHC	2 cut(s)	1285, 1651
Bsp1407I	TGTACA	1 cut(s)	1393
Bsp143I	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
BspACI	CCGC	3 cut(s)	872, 1254, 1613
BspCNI	CTCAG	7 cut(s)	73, 253, 855, 1147, 1282, 1329, 1415
BspLI	GGNNCC	3 cut(s)	400, 1371, 1384
BspMAI	CTGCAG	1 cut(s)	412
BspMI	ACCTGC	1 cut(s)	380
BspPI	GGATC	2 cut(s)	1197, 1619
BspT107I	GGYRCC	3 cut(s)	398, 1369, 1382
BsrDI	GCAATG	3 cut(s)	882, 1587, 1668
BsrGI	TGTACA	1 cut(s)	1393
BsrI	ACTGG	4 cut(s)	196, 519, 545, 915
BssMI	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
Bst2UI	CCWGG	3 cut(s)	758, 972, 1374
Bst4CI	ACNGT	2 cut(s)	505, 823
Bst6I	CTCTTC	2 cut(s)	41, 530
BstAPI	GCANNNNNTGC	1 cut(s)	1511
BstAUI	TGTACA	1 cut(s)	1393
BstC8I	GCNNGC	2 cut(s)	142, 1731
BstF5I	GGATG	3 cut(s)	184, 671, 1525
BstHHI	GCGC	1 cut(s)	146
BstKTI	GATC	9 cut(s)	525, 808, 816, 1081, 1182, 1192, 1309, 1603, 1627
BstMBI	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
BstMWI	GCNNNNNNNGC	4 cut(s)	464, 1484, 1511, 1670
BstNI	CCWGG	3 cut(s)	758, 972, 1374
BstNSI	RCATGY	1 cut(s)	736
BstSCI	CCNGG	4 cut(s)	756, 970, 1372, 1747
BstSFI	CTRYAG	3 cut(s)	408, 573, 630
BstSLI	GKGCMC	1 cut(s)	1651
BstV1I	GCAGC	3 cut(s)	861, 1336, 1673
BstV2I	GAAGAC	2 cut(s)	533, 1550
BstX2I	RGATCY	5 cut(s)	522, 813, 1189, 1600, 1624
BstYI	RGATCY	5 cut(s)	522, 813, 1189, 1600, 1624
Bsu36I	CCTNAGG	1 cut(s)	229
BsuI	GTATCC	2 cut(s)	722, 878
BtgZI	GCGATG	1 cut(s)	362
BtsCI	GGATG	3 cut(s)	184, 671, 1525
BtsI	GCAGTG	1 cut(s)	405
BtsIMutI	CAGTG	1 cut(s)	405
BveI	ACCTGC	1 cut(s)	380
Cac8I	GCNNGC	2 cut(s)	142, 1731
CaiI	CAGNNNCTG	1 cut(s)	1028
CfoI	GCGC	1 cut(s)	146
Cfr13I	GGNCC	3 cut(s)	233, 644, 793
Csp6I	GTAC	5 cut(s)	165, 399, 908, 1394, 1573
CviAII	CATG	1 cut(s)	733
CviQI	GTAC	5 cut(s)	165, 399, 908, 1394, 1573
DpnI	GATC	9 cut(s)	524, 807, 815, 1080, 1181, 1191, 1308, 1602, 1626
DpnII	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
Eam1104I	CTCTTC	2 cut(s)	41, 530
EarI	CTCTTC	2 cut(s)	41, 530
Eco32I	GATATC	2 cut(s)	156, 895
Eco47I	GGWCC	3 cut(s)	233, 644, 793
Eco57I	CTGAAG	2 cut(s)	645, 734
Eco81I	CCTNAGG	1 cut(s)	229
EcoO109I	RGGNCCY	2 cut(s)	233, 793
EcoRI	GAATTC	1 cut(s)	1426
EcoRII	CCWGG	3 cut(s)	756, 970, 1372
EcoRV	GATATC	2 cut(s)	156, 895
EcoT22I	ATGCAT	1 cut(s)	1213
FaeI	CATG	1 cut(s)	736
FatI	CATG	1 cut(s)	732
FauI	CCCGC	2 cut(s)	879, 1606
FauNDI	CATATG	1 cut(s)	1390
FbaI	TGATCA	1 cut(s)	805
Fnu4HI	GCNGC	3 cut(s)	875, 1350, 1662
FokI	GGATG	3 cut(s)	191, 658, 1532
Fsp4HI	GCNGC	3 cut(s)	875, 1350, 1662
FspBI	CTAG	2 cut(s)	797, 920
GlaI	GCGC	1 cut(s)	145
GluI	GCNGC	3 cut(s)	875, 1350, 1662
GsaI	CCCAGC	1 cut(s)	96
GsuI	CTGGAG	1 cut(s)	1062
HapII	CCGG	2 cut(s)	1674, 1748
HhaI	GCGC	1 cut(s)	146
Hin1II	CATG	1 cut(s)	736
Hin6I	GCGC	1 cut(s)	144
HinP1I	GCGC	1 cut(s)	144
HinfI	GANTC	5 cut(s)	113, 263, 538, 668, 1159
HpaII	CCGG	2 cut(s)	1674, 1748
HphI	GGTGA	2 cut(s)	404, 937
Hpy166II	GTNNAC	3 cut(s)	443, 928, 944
Hpy188III	TCNNGA	6 cut(s)	520, 526, 1041, 1183, 1221, 1641
Hpy8I	GTNNAC	3 cut(s)	443, 928, 944
HpyAV	CCTTC	8 cut(s)	22, 523, 549, 701, 1152, 1180, 1355, 1661
HpyCH4III	ACNGT	2 cut(s)	505, 823
HpyCH4IV	ACGT	3 cut(s)	244, 339, 689
HpyF10VI	GCNNNNNNNGC	4 cut(s)	464, 1484, 1511, 1670
HpySE526I	ACGT	3 cut(s)	244, 339, 689
Hsp92II	CATG	1 cut(s)	736
HspAI	GCGC	1 cut(s)	144
KpnI	GGTACC	1 cut(s)	402
Ksp22I	TGATCA	1 cut(s)	805
Kzo9I	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
LmnI	GCTCC	4 cut(s)	344, 464, 1280, 1492
Lsp1109I	GCAGC	3 cut(s)	861, 1336, 1673
LweI	GCATC	5 cut(s)	169, 202, 339, 1318, 1600
MaeI	CTAG	2 cut(s)	797, 920
MaeII	ACGT	3 cut(s)	244, 339, 689
MaeIII	GTNAC	2 cut(s)	295, 799
MalI	GATC	9 cut(s)	524, 807, 815, 1080, 1181, 1191, 1308, 1602, 1626
MboI	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
MfeI	CAATTG	4 cut(s)	300, 330, 663, 1500
MflI	RGATCY	5 cut(s)	522, 813, 1189, 1600, 1624
MhlI	GDGCHC	2 cut(s)	1285, 1651
MlyI	GAGTC	4 cut(s)	122, 272, 547, 677
Mph1103I	ATGCAT	1 cut(s)	1213
MroXI	GAANNNNTTC	2 cut(s)	834, 1410
MseI	TTAA	4 cut(s)	567, 948, 963, 1272
MspA1I	CMGCKG	2 cut(s)	874, 1733
MspI	CCGG	2 cut(s)	1674, 1748
MspR9I	CCNGG	4 cut(s)	758, 972, 1374, 1749
MunI	CAATTG	4 cut(s)	300, 330, 663, 1500
Mva1269I	GAATGC	1 cut(s)	176
MvaI	CCWGG	3 cut(s)	758, 972, 1374
MwoI	GCNNNNNNNGC	4 cut(s)	464, 1484, 1511, 1670
NciI	CCSGG	1 cut(s)	1749
NdeI	CATATG	1 cut(s)	1390
NdeII	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
NlaIII	CATG	1 cut(s)	736
NlaIV	GGNNCC	3 cut(s)	400, 1371, 1384
NsiI	ATGCAT	1 cut(s)	1213
NspI	RCATGY	1 cut(s)	736
PaqCI	CACCTGC	1 cut(s)	380
PciI	ACATGT	1 cut(s)	732
PctI	GAATGC	1 cut(s)	176
PdmI	GAANNNNTTC	2 cut(s)	834, 1410
PfeI	GAWTC	1 cut(s)	1159
PfoI	TCCNGGA	2 cut(s)	970, 1747
PkrI	GCNGC	3 cut(s)	876, 1351, 1663
PleI	GAGTC	4 cut(s)	121, 271, 546, 676
PpsI	GAGTC	4 cut(s)	121, 271, 546, 676
PpuMI	RGGWCCY	2 cut(s)	233, 793
PscI	ACATGT	1 cut(s)	732
PsiI	TTATAA	1 cut(s)	45
Psp5II	RGGWCCY	2 cut(s)	233, 793
Psp6I	CCWGG	3 cut(s)	756, 970, 1372
PspFI	CCCAGC	1 cut(s)	92
PspGI	CCWGG	3 cut(s)	756, 970, 1372
PspN4I	GGNNCC	3 cut(s)	400, 1371, 1384
PspPI	GGNCC	3 cut(s)	233, 644, 793
PspPPI	RGGWCCY	2 cut(s)	233, 793
PsrI	GAACNNNNNNTAC	2 cut(s)	288, 320
PstI	CTGCAG	1 cut(s)	412
PstNI	CAGNNNCTG	1 cut(s)	1028
PsuI	RGATCY	5 cut(s)	522, 813, 1189, 1600, 1624
PvuII	CAGCTG	1 cut(s)	1733
RsaI	GTAC	5 cut(s)	166, 400, 909, 1395, 1574
RsaNI	GTAC	5 cut(s)	165, 399, 908, 1394, 1573
SaqAI	TTAA	4 cut(s)	567, 948, 963, 1272
SatI	GCNGC	3 cut(s)	875, 1350, 1662
Sau3AI	GATC	9 cut(s)	522, 805, 813, 1078, 1179, 1189, 1306, 1600, 1624
Sau96I	GGNCC	3 cut(s)	233, 644, 793
ScaI	AGTACT	1 cut(s)	909
SchI	GAGTC	4 cut(s)	122, 272, 547, 677
ScrFI	CCNGG	4 cut(s)	758, 972, 1374, 1749
SduI	GDGCHC	2 cut(s)	1285, 1651
SfaNI	GCATC	5 cut(s)	169, 202, 339, 1318, 1600
SfcI	CTRYAG	3 cut(s)	408, 573, 630
SinI	GGWCC	3 cut(s)	233, 644, 793
SmlI	CTYRAG	2 cut(s)	1061, 1692
SmoI	CTYRAG	2 cut(s)	1061, 1692
SpeI	ACTAGT	1 cut(s)	919
SsiI	CCGC	3 cut(s)	872, 1254, 1613
SspI	AATATT	1 cut(s)	1173
SspMI	CTAG	2 cut(s)	797, 920
StyD4I	CCNGG	4 cut(s)	756, 970, 1372, 1747
TaaI	ACNGT	2 cut(s)	505, 823
TaiI	ACGT	3 cut(s)	247, 342, 692
TaqI	TCGA	1 cut(s)	1220
TatI	WGTACW	2 cut(s)	907, 1393
TfiI	GAWTC	1 cut(s)	1159
Tru1I	TTAA	4 cut(s)	567, 948, 963, 1272
Tru9I	TTAA	4 cut(s)	567, 948, 963, 1272
TscAI	CASTG	1 cut(s)	412
TseI	GCWGC	3 cut(s)	874, 1349, 1661
TspDTI	ATGAA	5 cut(s)	392, 525, 595, 736, 1611
TspRI	CASTG	1 cut(s)	412
VpaK11BI	GGWCC	3 cut(s)	233, 644, 793
XapI	RAATTY	2 cut(s)	1406, 1426
XceI	RCATGY	1 cut(s)	736
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1675
XmnI	GAANNNNTTC	2 cut(s)	834, 1410
XspI	CTAG	2 cut(s)	797, 920
ZrmI	AGTACT	1 cut(s)	909
Zsp2I	ATGCAT	1 cut(s)	1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.