AT5G18950

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007941
Physical Location & Seq
Reverse (-)
6328379 .. 6330189
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18950.1

Sequence Viewer

Length: 1452 bp
ATGAGTAGAGGTCAGTCGTATTTGATTAGCTTCTTTAGAAACCGAACTCGAAAGAACCCTAATACCCAGATACGGAGTCTAACTGTCGAGTCTCGTGATTGTGAATCCAAGCCCGATGAGCAAAAATCGGCTGTGAGTTATACGGAGATGGCTAAAACTGTGTCTACCATCATGAGAGAGAGACAGAGATGGCAACAAACTCTGGTATCTGATTTTCCTTCGTTTGATTTCGCTGATCCTCTGTTTTTCGGCGAACTGTTGAAATCTCAGAACAATGTTCTGTTTTCTCTGTGGTTTTTCCGGTGGTTGTGTTCCAATTATGATTACACACCTGGTCCTGTTTCTTTGAATATTCTCTTTGGTGCCCTTTTGGATGGAAAAGCCGTGAAAGCTGCGAAGAGTTTTCTCGATACCACTGGCTTTAAACCTGAGCCTACGTTATTAGAGCAATACGTGAAGTGTCTTAGTGAAGAAGGGTTGGTAGAGGAAGCTATTGAAGTGTATAATGTACTGAAAGATATGGGAATTAGTTCTTCAGTTGTGACTTGCAATTCTGTTTTATTAGGTTGCTTGAAAGCGAGGAAACTCGATCGGTTTTGGGAACTTCATAAGGAAATGGTAGAATCTGAGTTTGATTCAGAGAGGATTCGGTGTTTGATTCGAGCTTTGTGTGATGGTGGGGATGTTTCAGAAGGCTATGAGCTTTTGAAGCAGGGATTGAAACAAGGGCTTGATCCAGGACAGTATGTGTATGCTAAACTGATATCTGGATTCTGTGAGATTGGGAACTATGCTTGTATGTCGGAGGTTCTTCATACGATGATAGCGTGGAACCATTTCCCGAGTATGTATATATACCAGAAGATTATAAAGGGGTTGTGTATGAATAAGAAGCAGCTTGAGGCTTATTGTATATTCAAGAATCTGAAAGATAAAGGCTATGCACCTGATAGAGTTGTGTATACTACGATGATTCGAGGATTCTGTGAGAAGGGATGGTTAGGGAGCGCGAGGAAGCTTTGGTTTGAGATGATAAAGAAAGGGATGCGACCAAACGAGTTCGCTTACAATGTGATGATACACGGGCATTTTAAAAGAGGCGAAATTTCGCTTGTTGAAGCATTCTACAATGAGATGTTGAGAAATGGCTATGGAGGAACAATGTTAAGCTGCAATACGATGATAAAGGGTTTCTGCTCACATGGAAAATCAGACGAGGCCTTCGAGATTTTCAAGAATATGTCTGAAACCGGGGTTACACCGAATGCAATCACGTATAACGCTTTGATAAAAGGATTTTGCAAAGAAAACAAGGTTGAGAAAGGCTTGAAACTATACAAGGAGCTTAAGGCCTTAGGTTTGAAGCCTTCTGGTATGGCATATGCTGCACTAGTGAGAAATCTGAAGATGTCTGATTCTGTTGCAACTTCTTTAAATCTAGAGATTGTCTGA

Protein Analysis

483

Amino Acids

55.0

Weight (kDa)

8.7

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 281 - 329 1.3e-09 PPR repeat family
PPR_1 PF12854 312 - 344 4.8e-08 PPR repeat
PPR_2 PF13041 316 - 362 8.5e-13 PPR repeat family
PPR PF01535 319 - 348 4.8e-07 PPR repeat
PPR_2 PF13041 351 - 383 3.1e-06 PPR repeat family
PPR_long PF17177 376 - 466 4.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 381 - 433 3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 390 - 435 1.4e-17 PPR repeat family
PPR PF01535 390 - 418 1.1e-07 PPR repeat
PPR_1 PF12854 392 - 414 8.4e-07 PPR repeat
PPR_1 PF12854 418 - 448 6.2e-10 PPR repeat
PPR_2 PF13041 421 - 467 3.6e-11 PPR repeat family
PPR PF01535 424 - 453 9.7e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
AccB1I GGYRCC 1 cut(s) 362
AccI GTMKAC 2 cut(s) 164, 962
AccII CGCG 1 cut(s) 1010
AclWI GGATC 2 cut(s) 230, 728
AcsI RAATTY 1 cut(s) 1104
AcuI CTGAAG 2 cut(s) 519, 1424
AfaI GTAC 1 cut(s) 510
AfiI CCNNNNNNNGG 1 cut(s) 72
AflII CTTAAG 1 cut(s) 1346
AhlI ACTAGT 1 cut(s) 1390
AjnI CCWGG 2 cut(s) 331, 736
AluBI AGCT 9 cut(s) 30, 392, 491, 665, 703, 898, 1018, 1170, 1345
AluI AGCT 9 cut(s) 30, 392, 491, 665, 703, 898, 1018, 1170, 1345
Alw26I GTCTC 2 cut(s) 96, 175
AlwI GGATC 2 cut(s) 230, 728
Ama87I CYCGRG 1 cut(s) 841
AoxI GGCC 2 cut(s) 1218, 1350
ApeKI GCWGC 4 cut(s) 392, 895, 1170, 1385
ApoI RAATTY 1 cut(s) 1104
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
Asp700I GAANNNNTTC 2 cut(s) 529, 836
AspLEI GCGC 1 cut(s) 1010
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 1252
AvaI CYCGRG 1 cut(s) 841
AvaII GGWCC 1 cut(s) 335
AxyI CCTNAGG 1 cut(s) 1354
BaeGI GKGCMC 1 cut(s) 367
BaeI ACNNNNGTAYC 2 cut(s) 189, 222
BanI GGYRCC 1 cut(s) 362
BauI CACGAG 1 cut(s) 93
BbvI GCAGC 4 cut(s) 379, 907, 1157, 1372
BccI CCATC 6 cut(s) 142, 176, 183, 368, 668, 990
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 2 cut(s) 333, 738
BcnI CCSGG 1 cut(s) 1252
BcoDI GTCTC 2 cut(s) 96, 175
BcuI ACTAGT 1 cut(s) 1390
BfaI CTAG 2 cut(s) 1391, 1439
BfrI CTTAAG 1 cut(s) 1346
BisI GCNGC 4 cut(s) 393, 896, 1171, 1386
BlsI GCNGC 4 cut(s) 394, 897, 1172, 1387
Bme1390I CCNGG 3 cut(s) 333, 738, 1252
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 841
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 364, 833
BmrFI CCNGG 3 cut(s) 333, 738, 1252
BmsI GCATC 1 cut(s) 1035
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 920
BpuMI CCSGG 1 cut(s) 1252
BsaAI YACGTR 2 cut(s) 454, 1275
BsaJI CCNNGG 1 cut(s) 1251
BsaWI WCCGGW 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 421
Bse21I CCTNAGG 1 cut(s) 1354
BseBI CCWGG 2 cut(s) 333, 738
BseDI CCNNGG 1 cut(s) 1251
BseGI GGATG 4 cut(s) 379, 688, 1001, 1050
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 3 cut(s) 281, 420, 618
BseNI ACTGG 1 cut(s) 421
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 4 cut(s) 379, 907, 1157, 1372
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 1 cut(s) 1010
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 2 cut(s) 1220, 1352
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 841
BsiSI CCGG 2 cut(s) 301, 1251
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 2 cut(s) 96, 175
BsmI GAATGC 2 cut(s) 1121, 1270
BsnI GGCC 2 cut(s) 1220, 1352
BsoBI CYCGRG 1 cut(s) 841
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 3 cut(s) 235, 589, 733
BspANI GGCC 2 cut(s) 1220, 1352
BspCNI CTCAG 3 cut(s) 280, 421, 619
BspFNI CGCG 1 cut(s) 1010
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 364, 833
BspPI GGATC 2 cut(s) 230, 728
BspT107I GGYRCC 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 1346
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 1251
BssMI GATC 3 cut(s) 235, 589, 733
BssNAI GTATAC 1 cut(s) 963
BssSI CACGAG 1 cut(s) 93
Bst1107I GTATAC 1 cut(s) 963
Bst2BI CACGAG 1 cut(s) 93
Bst2UI CCWGG 2 cut(s) 333, 738
Bst4CI ACNGT 4 cut(s) 85, 160, 258, 744
Bst6I CTCTTC 1 cut(s) 392
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 1 cut(s) 1385
BstBAI YACGTR 2 cut(s) 454, 1275
BstDEI CTNAG 5 cut(s) 267, 429, 464, 627, 1354
BstF5I GGATG 4 cut(s) 379, 688, 1001, 1050
BstFNI CGCG 1 cut(s) 1010
BstHHI GCGC 1 cut(s) 1010
BstKTI GATC 3 cut(s) 238, 592, 736
BstMAI GTCTC 2 cut(s) 96, 175
BstMBI GATC 3 cut(s) 235, 589, 733
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 4 cut(s) 118, 389, 709, 1385
BstNI CCWGG 2 cut(s) 333, 738
BstSCI CCNGG 3 cut(s) 331, 736, 1250
BstSLI GKGCMC 1 cut(s) 367
BstUI CGCG 1 cut(s) 1010
BstV1I GCAGC 4 cut(s) 379, 907, 1157, 1372
BstZ17I GTATAC 1 cut(s) 963
Bsu36I CCTNAGG 1 cut(s) 1354
BsuRI GGCC 2 cut(s) 1220, 1352
BtsCI GGATG 4 cut(s) 379, 688, 1001, 1050
BtsIMutI CAGTG 1 cut(s) 414
CciI TCATGA 1 cut(s) 171
CfoI GCGC 1 cut(s) 1010
Cfr13I GGNCC 1 cut(s) 335
CsiI ACCWGGT 1 cut(s) 331
Csp6I GTAC 1 cut(s) 509
CviAII CATG 2 cut(s) 172, 1202
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 5 cut(s) 267, 429, 464, 627, 1354
DpnI GATC 3 cut(s) 237, 591, 735
DpnII GATC 3 cut(s) 235, 589, 733
DraI TTTAAA 3 cut(s) 424, 1093, 1434
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco147I AGGCCT 2 cut(s) 1220, 1352
Eco32I GATATC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 519, 1424
Eco81I CCTNAGG 1 cut(s) 1354
Eco88I CYCGRG 1 cut(s) 841
EcoRII CCWGG 2 cut(s) 331, 736
EcoRV GATATC 1 cut(s) 765
FaeI CATG 2 cut(s) 175, 1205
FatI CATG 2 cut(s) 171, 1201
FauNDI CATATG 1 cut(s) 1381
FblI GTMKAC 2 cut(s) 164, 962
Fnu4HI GCNGC 4 cut(s) 393, 896, 1171, 1386
FokI GGATG 4 cut(s) 386, 695, 1008, 1057
Fsp4HI GCNGC 4 cut(s) 393, 896, 1171, 1386
FspBI CTAG 2 cut(s) 1391, 1439
GlaI GCGC 1 cut(s) 1009
GluI GCNGC 4 cut(s) 393, 896, 1171, 1386
HaeIII GGCC 2 cut(s) 1220, 1352
HapII CCGG 2 cut(s) 301, 1251
HhaI GCGC 1 cut(s) 1010
Hin1II CATG 2 cut(s) 175, 1205
Hin6I GCGC 1 cut(s) 1008
HinP1I GCGC 1 cut(s) 1008
HindIII AAGCTT 1 cut(s) 1016
HpaII CCGG 2 cut(s) 301, 1251
Hpy166II GTNNAC 2 cut(s) 165, 963
Hpy188III TCNNGA 9 cut(s) 95, 172, 407, 768, 841, 919, 1225, 1234, 1439
Hpy8I GTNNAC 2 cut(s) 165, 963
HpyAV CCTTC 6 cut(s) 228, 467, 686, 985, 1231, 1377
HpyCH4III ACNGT 4 cut(s) 85, 160, 258, 744
HpyCH4IV ACGT 3 cut(s) 437, 453, 1274
HpyCH4V TGCA 7 cut(s) 549, 944, 1173, 1268, 1302, 1388, 1424
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 389, 709, 1385
HpyF3I CTNAG 5 cut(s) 267, 429, 464, 627, 1354
HpySE526I ACGT 3 cut(s) 437, 453, 1274
Hsp92II CATG 2 cut(s) 175, 1205
HspAI GCGC 1 cut(s) 1008
Kzo9I GATC 3 cut(s) 235, 589, 733
LmnI GCTCC 2 cut(s) 1005, 1342
Lsp1109I GCAGC 4 cut(s) 379, 907, 1157, 1372
LweI GCATC 1 cut(s) 1035
MabI ACCWGGT 1 cut(s) 331
MaeI CTAG 2 cut(s) 1391, 1439
MaeII ACGT 3 cut(s) 437, 453, 1274
MaeIII GTNAC 2 cut(s) 541, 1255
MalI GATC 3 cut(s) 237, 591, 735
MboI GATC 3 cut(s) 235, 589, 733
MboII GAAGA 6 cut(s) 409, 482, 525, 803, 874, 1417
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 4 cut(s) 316, 525, 550, 1104
MlyI GAGTC 2 cut(s) 85, 98
MmeI TCCRAC 1 cut(s) 783
MroXI GAANNNNTTC 2 cut(s) 529, 836
MseI TTAA 5 cut(s) 423, 1092, 1166, 1347, 1433
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 2 cut(s) 301, 1251
MspR9I CCNGG 3 cut(s) 333, 738, 1252
Mva1269I GAATGC 2 cut(s) 1121, 1270
MvaI CCWGG 2 cut(s) 333, 738
MvnI CGCG 1 cut(s) 1010
MwoI GCNNNNNNNGC 4 cut(s) 118, 389, 709, 1385
NciI CCSGG 1 cut(s) 1252
NdeI CATATG 1 cut(s) 1381
NdeII GATC 3 cut(s) 235, 589, 733
NlaIII CATG 2 cut(s) 175, 1205
NlaIV GGNNCC 2 cut(s) 364, 833
NmuCI GTSAC 1 cut(s) 541
PagI TCATGA 1 cut(s) 171
PceI AGGCCT 2 cut(s) 1220, 1352
PcsI WCGNNNNNNNCGW 2 cut(s) 824, 1221
PctI GAATGC 2 cut(s) 1121, 1270
PdmI GAANNNNTTC 2 cut(s) 529, 836
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 4 cut(s) 394, 897, 1172, 1387
Ple19I CGATCG 1 cut(s) 592
PleI GAGTC 2 cut(s) 84, 97
PpsI GAGTC 2 cut(s) 84, 97
Ppu21I YACGTR 2 cut(s) 454, 1275
PsiI TTATAA 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 331, 736
PspGI CCWGG 2 cut(s) 331, 736
PspN4I GGNNCC 2 cut(s) 364, 833
PspPI GGNCC 1 cut(s) 335
PvuI CGATCG 1 cut(s) 592
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
SaqAI TTAA 5 cut(s) 423, 1092, 1166, 1347, 1433
SatI GCNGC 4 cut(s) 393, 896, 1171, 1386
Sau3AI GATC 3 cut(s) 235, 589, 733
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 2 cut(s) 85, 98
ScrFI CCNGG 3 cut(s) 333, 738, 1252
SduI GDGCHC 1 cut(s) 367
SexAI ACCWGGT 1 cut(s) 331
SfaNI GCATC 1 cut(s) 1035
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 899, 1346
SmoI CTYRAG 2 cut(s) 899, 1346
SpeI ACTAGT 1 cut(s) 1390
Sse9I AATT 4 cut(s) 316, 525, 550, 1104
SseBI AGGCCT 2 cut(s) 1220, 1352
SspI AATATT 1 cut(s) 352
SspMI CTAG 2 cut(s) 1391, 1439
StuI AGGCCT 2 cut(s) 1220, 1352
StyD4I CCNGG 3 cut(s) 331, 736, 1250
TaaI ACNGT 4 cut(s) 85, 160, 258, 744
TaiI ACGT 3 cut(s) 440, 456, 1277
TaqI TCGA 7 cut(s) 49, 87, 408, 588, 661, 976, 1224
TasI AATT 4 cut(s) 316, 525, 550, 1104
TatI WGTACW 1 cut(s) 508
Tru1I TTAA 5 cut(s) 423, 1092, 1166, 1347, 1433
Tru9I TTAA 5 cut(s) 423, 1092, 1166, 1347, 1433
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 4 cut(s) 392, 895, 1170, 1385
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 3 cut(s) 596, 803, 899
TspGWI ACGGA 2 cut(s) 88, 158
TspRI CASTG 1 cut(s) 421
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 1104
XbaI TCTAGA 1 cut(s) 1438
XmiI GTMKAC 2 cut(s) 164, 962
XmnI GAANNNNTTC 2 cut(s) 529, 836
XspI CTAG 2 cut(s) 1391, 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.