RchiOBHm_Chr4g0419121

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007941
Physical Location & Seq
Reverse (-)
44631233 .. 44632922
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38906

Sequence Viewer

Length: 1425 bp
ATGGCCAGAGCCTCATCTTCAATCTTAATATTCCTCCGCCAAAACCCTCGTGCTCTCAAAAAACCCAATACCCAAATCCGATACCTTACCGTAGAAACCAAAGATTGCGACTTTACAGAAGTTGCCCAACAGATTTGTAAGATCATTAGAACAAAACCCAGATGGGAGAACACTCTGTCCTCTGAGTACCCTTCTTTAAATTTCTCTGATCCTCGGTTTATCCGTGAGGTTTTGAAGCAGACCAGTGTGCTCCTTTCGGTTCGGTTCTTCTTGTGGTTGAGCTCTCGAAAAGGGTTTTCGCCAGACCCCATTTCGTGTAATGCGGTTTTCAATGCTCTTGTGGAGGCTAAGGCCTGCAGTGCAGCACAATCTTTTCTTAAGTCTACGGGTTTTAGCCCCGAGCCGGTTTCATTGGAAAGTTATGCTCGGTGTCTTTGGGAGGCTGGGAGGATTAAGGAAGCCTGTGATGTGTTTGAGAGGTTGAAAGGGGCTGGAGTGTGCCCGTCGATTGGGACTTGGAATGCGGCTTTGTCAGGGTGCCTGAAGGCTAAGAGGACAGATATGGTTTGGAAGTTGTATCAAGAAATGATGGAATATGGTGTTGTAGCTGATGTTGAGACTGTTGGGTGTCTCATTCGAGGATTTTGTGATGATAATGAGGTATTCAAAGGTTATGGACTTCTTAGTCAGGTTTTGGAAGATGGAGTAGTCCCTGGAAAAGCTGCTTTCAATCGGTTGATATCTGAGTTTTGTAAGGAGAAGGAATATGCTAAAGTGTCTGAACTCCTCCATACAATGATAGTGATGAACTGTGCTCCCGATAATTTTACTTATCAGGAGGTAATCAATTGGTTGTGCAAGAAAGGAAGGCCATATGAGGGTTTTCGGATTTTCAATGATCTTAAGTATAGAGGCTATGCTCCTGATATTGTCATGTACACAACCATGATTCATGGTCTTTGTACGATGGGTTGGCTTGGAGAAGCTAGGAAGCTATGGTTTGAGATGATTGATAAGGGATTTCATCCAAGCAAGTACACTTGCAACACCCTAATTCATGGGTTTTGTAAGAGTGGAATTTTAAAACAGGCCCAGATGTGCAGGGAGATGTGTGATAAGGGATTTCGTCCTAGCAAGTACACTAACAATACCATGATTCATGGGTTTTGTAAGAGTGGAAATTTAGAACAGGCCTGGATCTTGTGCAAGGAGATGGGTGATAAGGGATTTCGTCCAAGCAAGTACATTTACGATATCATGATTCATGGGTTTTGTAAGAGTGGAAATTTCGAACAGGCCAGGATCATTTGCAAGGAGATGTGTGATAAAGGTTATATGGAAACCACAATCAATCGAAACGCAATGATGACAGGGCTTTGTTTACATGGAAGGACCGGTCAGGCATATAAGCTGTTTAGAAAATAG

Protein Analysis

474

Amino Acids

54.08

Weight (kDa)

8.95

Isoelectric Point (pI)

29.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 168 - 217 3.2e-11 PPR repeat family
PPR_2 PF13041 241 - 287 2.1e-09 PPR repeat family
PPR_2 PF13041 281 - 321 3.7e-10 PPR repeat family
PPR_1 PF12854 304 - 336 8.3e-09 PPR repeat
PPR_2 PF13041 308 - 357 6.2e-15 PPR repeat family
PPR PF01535 311 - 341 2.6e-07 PPR repeat
PPR_2 PF13041 347 - 391 2.1e-10 PPR repeat family
PPR_1 PF12854 373 - 405 2e-07 PPR repeat
PPR_2 PF13041 381 - 426 1.8e-13 PPR repeat family
PPR PF01535 383 - 410 9.5e-06 PPR repeat
PPR_1 PF12854 408 - 440 6.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 537
AccI GTMKAC 1 cut(s) 383
AciI CCGC 3 cut(s) 37, 323, 524
AclWI GGATC 3 cut(s) 203, 1205, 1310
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 199, 1077, 1180, 1285
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 6 cut(s) 188, 938, 964, 1037, 1139, 1244
AfiI CCNNNNNNNGG 3 cut(s) 403, 509, 878
AflII CTTAAG 2 cut(s) 377, 902
AgeI ACCGGT 1 cut(s) 1394
AgsI TTSAA 7 cut(s) 21, 235, 331, 484, 667, 730, 895
AjnI CCWGG 3 cut(s) 712, 1193, 1298
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 6 cut(s) 282, 608, 722, 986, 994, 1411
AluI AGCT 6 cut(s) 282, 608, 722, 986, 994, 1411
Alw21I GWGCWC 4 cut(s) 55, 252, 284, 817
Alw26I GTCTC 2 cut(s) 611, 635
AlwI GGATC 3 cut(s) 203, 1205, 1310
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 6 cut(s) 3, 351, 869, 1089, 1191, 1296
ApeKI GCWGC 2 cut(s) 362, 722
ApoI RAATTY 4 cut(s) 199, 1077, 1180, 1285
AsiGI ACCGGT 1 cut(s) 1394
AspS9I GGNCC 2 cut(s) 1090, 1392
AsuHPI GGTGA 1 cut(s) 1229
AsuII TTCGAA 1 cut(s) 1290
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 1392
BaeGI GKGCMC 1 cut(s) 503
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 537
BanII GRGCYC 1 cut(s) 284
BauI CACGAG 1 cut(s) 48
Bbv12I GWGCWC 4 cut(s) 55, 252, 284, 817
BbvI GCAGC 2 cut(s) 374, 709
BccI CCATC 5 cut(s) 156, 583, 695, 961, 1207
BciT130I CCWGG 3 cut(s) 714, 1195, 1300
BcoDI GTCTC 2 cut(s) 611, 635
BfaI CTAG 2 cut(s) 987, 1131
BfmI CTRYAG 1 cut(s) 355
BfrI CTTAAG 2 cut(s) 377, 902
BisI GCNGC 3 cut(s) 363, 525, 723
BlsI GCNGC 3 cut(s) 364, 526, 724
Bme1390I CCNGG 3 cut(s) 714, 1195, 1300
Bme18I GGWCC 1 cut(s) 1392
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 1090, 1392
BmiI GGNNCC 1 cut(s) 539
BmrFI CCNGG 3 cut(s) 714, 1195, 1300
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 1290
BsaJI CCNNGG 2 cut(s) 212, 712
BsaWI WCCGGW 1 cut(s) 1394
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 509, 878
Bse118I RCCGGY 2 cut(s) 403, 1394
Bse1I ACTGG 1 cut(s) 243
Bse3DI GCAATG 1 cut(s) 1368
BseBI CCWGG 3 cut(s) 714, 1195, 1300
BseDI CCNNGG 2 cut(s) 212, 712
BseGI GGATG 1 cut(s) 1024
BseLI CCNNNNNNNGG 3 cut(s) 403, 509, 878
BseMI GCAATG 1 cut(s) 1368
BseMII CTCAG 2 cut(s) 174, 735
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 776
BseSI GKGCMC 1 cut(s) 503
BseXI GCAGC 2 cut(s) 374, 709
BseYI CCCAGC 1 cut(s) 443
BsgI GTGCAG 2 cut(s) 381, 1120
BshFI GGCC 6 cut(s) 5, 353, 871, 1091, 1193, 1298
BshNI GGYRCC 1 cut(s) 537
BshTI ACCGGT 1 cut(s) 1394
BsiHKAI GWGCWC 4 cut(s) 55, 252, 284, 817
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 2 cut(s) 404, 1395
BslFI GGGAC 2 cut(s) 526, 695
BslI CCNNNNNNNGG 3 cut(s) 403, 509, 878
BsmAI GTCTC 2 cut(s) 611, 635
BsmFI GGGAC 2 cut(s) 526, 695
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 6 cut(s) 5, 353, 871, 1091, 1193, 1298
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 1290
Bsp1286I GDGCHC 5 cut(s) 55, 252, 284, 503, 817
Bsp1407I TGTACA 1 cut(s) 936
Bsp143I GATC 5 cut(s) 141, 208, 898, 1197, 1302
BspACI CCGC 3 cut(s) 37, 323, 524
BspANI GGCC 6 cut(s) 5, 353, 871, 1091, 1193, 1298
BspCNI CTCAG 2 cut(s) 175, 736
BspHI TCATGA 1 cut(s) 1257
BspLI GGNNCC 1 cut(s) 539
BspMAI CTGCAG 1 cut(s) 359
BspPI GGATC 3 cut(s) 203, 1205, 1310
BspT104I TTCGAA 1 cut(s) 1290
BspT107I GGYRCC 1 cut(s) 537
BspTI CTTAAG 2 cut(s) 377, 902
BsrDI GCAATG 1 cut(s) 1368
BsrFI RCCGGY 2 cut(s) 403, 1394
BsrGI TGTACA 1 cut(s) 936
BsrI ACTGG 1 cut(s) 243
BssAI RCCGGY 2 cut(s) 403, 1394
BssECI CCNNGG 2 cut(s) 212, 712
BssMI GATC 5 cut(s) 141, 208, 898, 1197, 1302
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 3 cut(s) 714, 1195, 1300
Bst4CI ACNGT 3 cut(s) 91, 622, 812
BstAFI CTTAAG 2 cut(s) 377, 902
BstAUI TGTACA 1 cut(s) 936
BstBI TTCGAA 1 cut(s) 1290
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 5 cut(s) 183, 348, 549, 683, 744
BstF5I GGATG 1 cut(s) 1024
BstKTI GATC 5 cut(s) 144, 211, 901, 1200, 1305
BstMAI GTCTC 2 cut(s) 611, 635
BstMBI GATC 5 cut(s) 141, 208, 898, 1197, 1302
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 3 cut(s) 714, 1195, 1300
BstSCI CCNGG 3 cut(s) 712, 1193, 1298
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 503
BstV1I GCAGC 2 cut(s) 374, 709
BstX2I RGATCY 1 cut(s) 1197
BstYI RGATCY 1 cut(s) 1197
BsuRI GGCC 6 cut(s) 5, 353, 871, 1091, 1193, 1298
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 2 cut(s) 250, 364
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 1 cut(s) 1257
Cfr10I RCCGGY 2 cut(s) 403, 1394
Cfr13I GGNCC 2 cut(s) 1090, 1392
Csp6I GTAC 6 cut(s) 187, 937, 963, 1036, 1138, 1243
CspAI ACCGGT 1 cut(s) 1394
CviAII CATG 9 cut(s) 934, 946, 953, 1058, 1153, 1160, 1258, 1265, 1385
CviQI GTAC 6 cut(s) 187, 937, 963, 1036, 1138, 1243
DdeI CTNAG 5 cut(s) 183, 348, 549, 683, 744
DpnI GATC 5 cut(s) 143, 210, 900, 1199, 1304
DpnII GATC 5 cut(s) 141, 208, 898, 1197, 1302
DraI TTTAAA 2 cut(s) 198, 1083
DrdI GACNNNNNNGTC 1 cut(s) 684
DseDI GACNNNNNNGTC 1 cut(s) 684
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 282
Eco147I AGGCCT 2 cut(s) 353, 1193
Eco24I GRGCYC 1 cut(s) 284
Eco32I GATATC 2 cut(s) 741, 1255
Eco47I GGWCC 1 cut(s) 1392
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 563
Eco88I CYCGRG 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 282
EcoRII CCWGG 3 cut(s) 712, 1193, 1298
EcoRV GATATC 2 cut(s) 741, 1255
EcoT38I GRGCYC 1 cut(s) 284
FaeI CATG 9 cut(s) 937, 949, 956, 1061, 1156, 1163, 1261, 1268, 1388
FaqI GGGAC 2 cut(s) 526, 695
FatI CATG 9 cut(s) 933, 945, 952, 1057, 1152, 1159, 1257, 1264, 1384
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 3 cut(s) 363, 525, 723
FokI GGATG 1 cut(s) 1011
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 3 cut(s) 363, 525, 723
FspBI CTAG 2 cut(s) 987, 1131
GluI GCNGC 3 cut(s) 363, 525, 723
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 6 cut(s) 5, 353, 871, 1091, 1193, 1298
HapII CCGG 2 cut(s) 404, 1395
Hin1II CATG 9 cut(s) 937, 949, 956, 1061, 1156, 1163, 1261, 1268, 1388
HinfI GANTC 3 cut(s) 949, 1156, 1261
HpaII CCGG 2 cut(s) 404, 1395
HphI GGTGA 1 cut(s) 1229
Hpy166II GTNNAC 5 cut(s) 384, 939, 1038, 1140, 1382
Hpy188I TCNGA 6 cut(s) 80, 184, 208, 745, 781, 888
Hpy188III TCNNGA 6 cut(s) 285, 581, 818, 836, 923, 1258
Hpy8I GTNNAC 5 cut(s) 384, 939, 1038, 1140, 1382
Hpy99I CGWCG 1 cut(s) 508
HpyAV CCTTC 5 cut(s) 201, 538, 754, 861, 1383
HpyCH4III ACNGT 3 cut(s) 91, 622, 812
HpyCH4V TGCA 7 cut(s) 357, 362, 858, 1044, 1101, 1206, 1311
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 5 cut(s) 183, 348, 549, 683, 744
Hsp92II CATG 9 cut(s) 937, 949, 956, 1061, 1156, 1163, 1261, 1268, 1388
Kzo9I GATC 5 cut(s) 141, 208, 898, 1197, 1302
LmnI GCTCC 3 cut(s) 255, 820, 925
Lsp1109I GCAGC 2 cut(s) 374, 709
MaeI CTAG 2 cut(s) 987, 1131
MalI GATC 5 cut(s) 143, 210, 900, 1199, 1304
MboI GATC 5 cut(s) 141, 208, 898, 1197, 1302
MboII GAAGA 3 cut(s) 9, 259, 710
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 1 cut(s) 1197
MhlI GDGCHC 5 cut(s) 55, 252, 284, 503, 817
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 199, 823, 847, 1053, 1077, 1180, 1285
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 26, 197, 378, 453, 903, 1082
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 2 cut(s) 377, 902
MspI CCGG 2 cut(s) 404, 1395
MspR9I CCNGG 3 cut(s) 714, 1195, 1300
MunI CAATTG 1 cut(s) 847
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 3 cut(s) 714, 1195, 1300
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeI CATATG 1 cut(s) 874
NdeII GATC 5 cut(s) 141, 208, 898, 1197, 1302
NlaIII CATG 9 cut(s) 937, 949, 956, 1061, 1156, 1163, 1261, 1268, 1388
NlaIV GGNNCC 1 cut(s) 539
NspV TTCGAA 1 cut(s) 1290
PagI TCATGA 1 cut(s) 1257
PceI AGGCCT 2 cut(s) 353, 1193
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 949, 1156, 1261
PinAI ACCGGT 1 cut(s) 1394
PkrI GCNGC 3 cut(s) 364, 526, 724
Psp124BI GAGCTC 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 712, 1193, 1298
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 3 cut(s) 712, 1193, 1298
PspN4I GGNNCC 1 cut(s) 539
PspPI GGNCC 2 cut(s) 1090, 1392
PstI CTGCAG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 1197
RsaI GTAC 6 cut(s) 188, 938, 964, 1037, 1139, 1244
RsaNI GTAC 6 cut(s) 187, 937, 963, 1036, 1138, 1243
RseI CAYNNNNRTG 1 cut(s) 944
SacI GAGCTC 1 cut(s) 284
SaqAI TTAA 6 cut(s) 26, 197, 378, 453, 903, 1082
SatI GCNGC 3 cut(s) 363, 525, 723
Sau3AI GATC 5 cut(s) 141, 208, 898, 1197, 1302
Sau96I GGNCC 2 cut(s) 1090, 1392
ScrFI CCNGG 3 cut(s) 714, 1195, 1300
SduI GDGCHC 5 cut(s) 55, 252, 284, 503, 817
SfcI CTRYAG 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 1290
SinI GGWCC 1 cut(s) 1392
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 2 cut(s) 377, 902
SmoI CTYRAG 2 cut(s) 377, 902
Sse9I AATT 7 cut(s) 199, 823, 847, 1053, 1077, 1180, 1285
SseBI AGGCCT 2 cut(s) 353, 1193
SsiI CCGC 3 cut(s) 37, 323, 524
SspI AATATT 1 cut(s) 30
SspMI CTAG 2 cut(s) 987, 1131
SstI GAGCTC 1 cut(s) 284
StuI AGGCCT 2 cut(s) 353, 1193
StyD4I CCNGG 3 cut(s) 712, 1193, 1298
TaaI ACNGT 3 cut(s) 91, 622, 812
TaqI TCGA 5 cut(s) 286, 506, 637, 1290, 1354
TasI AATT 7 cut(s) 199, 823, 847, 1053, 1077, 1180, 1285
TatI WGTACW 4 cut(s) 936, 1035, 1137, 1242
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 3 cut(s) 949, 1156, 1261
Tru1I TTAA 6 cut(s) 26, 197, 378, 453, 903, 1082
Tru9I TTAA 6 cut(s) 26, 197, 378, 453, 903, 1082
TscAI CASTG 2 cut(s) 250, 364
TseI GCWGC 2 cut(s) 362, 722
TspDTI ATGAA 7 cut(s) 399, 821, 941, 1013, 1046, 1148, 1253
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 2 cut(s) 250, 364
Vha464I CTTAAG 2 cut(s) 377, 902
VpaK11BI GGWCC 1 cut(s) 1392
XapI RAATTY 4 cut(s) 199, 1077, 1180, 1285
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 2 cut(s) 987, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.