AT5G19290

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014337
Physical Location & Seq
Forward (+)
6493826 .. 6495393
1568 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19290.1

Sequence Viewer

Length: 993 bp
ATGGGGTTGCATCCAATTTCCGACGCCAATGAACACAATCCCTTCGGCTCTCTCACCGCCGACGAATTCTACGCCAAACATTCCGTTAGCCACTCCTCCGCTTTCATCACCAACCCTCGCGGTCTCAAACTCTTCACTCAATGGTGGTCACCACTCCCTCCAACTAAACCAATCGGTATCATCGCTGTCGTTCATGGCTTCACCGGCGAATCCAGTTGGTTCCTTCAGCTCACATCAATCCTCTTCGCTAAATCCGGTTTCATAACCTGCGCAATTGATCACCAAGGCCATGGATTCTCCGATGGACTCATCGCTCATATCCCTGACATCAATCCCGTCGTCGATGACTGTATCTCTTTCTTCGATGACTTCCGTAGCCGTCAAACACCGTCAGATCTGCCGTGTTTTCTCTACTCTGAATCCCTAGGCGGCGCGATTGCTCTCTACATCTCGCTTCGTCAGAGAGGTGTTTGGGATGGACTTATCCTCAACGGAGCTATGTGTGGAATCAGCGATAAATTCAAACCGCCGTGGCCGTTGGAGCATTTGCTATTCGTCGTCGCGAATCTTATCCCTACTTGGCGCGTTATCCCCACTCGCGGATCTATTCCCGATGTTTCGTTCAAGGAGCCGTGGAAGAGGAAGCTTGCCATGGCTAGCCCAAGGAGGACGGTGGCGAAACCACGGGCCGCTACTGCTTATGAGCTGATTCGTGTTTGTAAGGATCTGCAGGGGAGGTTTGAGGAAGTGGAGGTTCCGCTTCTGATTGTGCACGGCGGAGGTGATGTTGTATGCGACGTAGCGTGTGTTGAGGAGCTTCATCGGAGAGCGATTAGTGAGGATAAGACGATCAAGATCTACCCTGAGTTGTGGCATCAGATGATTGGGGAATCGGAGGAGAAAGTCGATCTGGTTTACGGTGATATGCTGAGCTGGCTCAAGAGTCGAGCTGAAAGGAAGGCACGCGCCGCCGTTGATGGAGGAGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

330

Amino Acids

36.66

Weight (kDa)

6.31

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 56 - 299 3.3e-56 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 63 - 292 2.9e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14980 AT5G19290
fragaria_vesca FvH4_7g19610
malus_domestica MD01G1108700.v1.1 MD07G1174800.v1.1
prunus_persica Prupe.2G213900_v2.0.a1
pyrus_communis pycom01g13570 pycom07g16920
rosa_chinensis RchiOBHm_Chr1g0363421
rosa_laevigata RLG00000027569
rosa_multiflora Rmu_sc0000339.1_g000010 Rmu_sc0004458.1_g000006
rosa_rugosa Rorug01G0306600
rosa_samantha Rh1AG315700 Rh1CG295400 Rh1DG310100
rosa_wichuraiana Rw1G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 275
AccII CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
AclWI GGATC 2 cut(s) 610, 732
AcoI YGGCCR 1 cut(s) 533
AcsI RAATTY 2 cut(s) 65, 518
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 2 cut(s) 523, 625
AluBI AGCT 8 cut(s) 229, 497, 646, 706, 817, 933, 950, 989
AluI AGCT 8 cut(s) 229, 497, 646, 706, 817, 933, 950, 989
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 2 cut(s) 610, 732
AoxI GGCC 3 cut(s) 286, 533, 687
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 1 cut(s) 986
ApoI RAATTY 2 cut(s) 65, 518
AspA2I CCTAGG 1 cut(s) 424
AspLEI GCGC 4 cut(s) 272, 434, 585, 968
AspS9I GGNCC 1 cut(s) 687
AsuHPI GGTGA 7 cut(s) 46, 100, 141, 193, 272, 794, 932
AsuNHI GCTAGC 1 cut(s) 656
AvrII CCTAGG 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 774
Bbv12I GWGCWC 1 cut(s) 774
BccI CCATC 3 cut(s) 296, 470, 971
BceAI ACGGC 7 cut(s) 363, 385, 514, 520, 616, 790, 956
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 2 cut(s) 425, 657
BfmI CTRYAG 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 275
BglII AGATCT 2 cut(s) 394, 855
BisI GCNGC 4 cut(s) 430, 690, 969, 987
BlnI CCTAGG 1 cut(s) 424
BlpI GCTNAGC 1 cut(s) 929
BlsI GCNGC 4 cut(s) 431, 691, 970, 988
BmgT120I GGNCC 1 cut(s) 687
BmiI GGNNCC 3 cut(s) 221, 630, 756
BmsI GCATC 2 cut(s) 19, 883
BmtI GCTAGC 1 cut(s) 660
Bpu1102I GCTNAGC 1 cut(s) 929
BpuEI CTTGAG 1 cut(s) 923
BsaBI GATNNNNATC 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 8 cut(s) 283, 289, 424, 530, 632, 651, 662, 683
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 854
BseDI CCNNGG 8 cut(s) 283, 289, 424, 530, 632, 651, 662, 683
BseGI GGATG 2 cut(s) 10, 481
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 855, 920
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 3 cut(s) 85, 827, 911
BseSI GKGCMC 1 cut(s) 774
Bsh1236I CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
BshFI GGCC 3 cut(s) 288, 535, 689
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 2 cut(s) 204, 255
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 3 cut(s) 288, 535, 689
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 774
Bsp143I GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
Bsp1720I GCTNAGC 1 cut(s) 929
Bsp19I CCATGG 2 cut(s) 289, 651
Bsp68I TCGCGA 1 cut(s) 563
BspANI GGCC 3 cut(s) 288, 535, 689
BspCNI CTCAG 2 cut(s) 856, 921
BspFNI CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
BspLI GGNNCC 3 cut(s) 221, 630, 756
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 2 cut(s) 610, 732
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 8 cut(s) 283, 289, 424, 530, 632, 651, 662, 683
BssMI GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 5 cut(s) 283, 289, 424, 651, 662
Bst4CI ACNGT 4 cut(s) 350, 390, 673, 920
Bst6I CTCTTC 3 cut(s) 137, 248, 632
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 648, 658, 935, 964
BstDEI CTNAG 3 cut(s) 864, 929, 990
BstDSI CCRYGG 5 cut(s) 289, 530, 632, 651, 683
BstEII GGTNACC 1 cut(s) 147
BstF5I GGATG 2 cut(s) 10, 481
BstFNI CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
BstHHI GCGC 4 cut(s) 272, 434, 585, 968
BstKTI GATC 7 cut(s) 280, 397, 605, 727, 852, 858, 910
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
BstMWI GCNNNNNNNGC 5 cut(s) 204, 541, 695, 934, 968
BstPI GGTNACC 1 cut(s) 147
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 1 cut(s) 774
BstUI CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
BstX2I RGATCY 4 cut(s) 394, 602, 724, 855
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 4 cut(s) 394, 602, 724, 855
BsuRI GGCC 3 cut(s) 288, 535, 689
BtgI CCRYGG 5 cut(s) 289, 530, 632, 651, 683
BtgZI GCGATG 2 cut(s) 166, 295
BtsCI GGATG 2 cut(s) 10, 481
BtuMI TCGCGA 1 cut(s) 563
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 4 cut(s) 648, 658, 935, 964
CfoI GCGC 4 cut(s) 272, 434, 585, 968
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 687
CseI GACGC 1 cut(s) 32
CviAII CATG 3 cut(s) 194, 290, 652
DdeI CTNAG 3 cut(s) 864, 929, 990
DpnI GATC 7 cut(s) 279, 396, 604, 726, 851, 857, 909
DpnII GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
EaeI YGGCCR 1 cut(s) 533
Eam1104I CTCTTC 3 cut(s) 137, 248, 632
EarI CTCTTC 3 cut(s) 137, 248, 632
EciI GGCGGA 1 cut(s) 792
Eco130I CCWWGG 5 cut(s) 283, 289, 424, 651, 662
Eco31I GGTCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 147
EcoO65I GGTNACC 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 65
EcoT14I CCWWGG 5 cut(s) 283, 289, 424, 651, 662
ErhI CCWWGG 5 cut(s) 283, 289, 424, 651, 662
FaeI CATG 3 cut(s) 197, 293, 655
FaiI YATR 9 cut(s) 195, 263, 291, 318, 500, 653, 702, 793, 926
FatI CATG 3 cut(s) 193, 289, 651
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 430, 690, 969, 987
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 430, 690, 969, 987
FspBI CTAG 2 cut(s) 425, 657
FspI TGCGCA 1 cut(s) 271
GlaI GCGC 4 cut(s) 271, 433, 584, 967
GluI GCNGC 4 cut(s) 430, 690, 969, 987
HaeIII GGCC 3 cut(s) 288, 535, 689
HapII CCGG 2 cut(s) 204, 255
HgaI GACGC 1 cut(s) 32
HhaI GCGC 4 cut(s) 272, 434, 585, 968
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 3 cut(s) 197, 293, 655
Hin6I GCGC 4 cut(s) 270, 432, 583, 966
HinP1I GCGC 4 cut(s) 270, 432, 583, 966
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 9 cut(s) 209, 294, 306, 419, 507, 565, 709, 890, 943
HpaII CCGG 2 cut(s) 204, 255
HphI GGTGA 7 cut(s) 46, 100, 141, 193, 272, 794, 932
Hpy166II GTNNAC 2 cut(s) 772, 916
Hpy188I TCNGA 9 cut(s) 22, 301, 394, 418, 462, 765, 825, 879, 895
Hpy188III TCNNGA 4 cut(s) 562, 611, 853, 940
Hpy8I GTNNAC 2 cut(s) 772, 916
Hpy99I CGWCG 7 cut(s) 26, 65, 341, 344, 560, 563, 800
HpyAV CCTTC 3 cut(s) 52, 233, 952
HpyCH4III ACNGT 4 cut(s) 350, 390, 673, 920
HpyCH4IV ACGT 1 cut(s) 798
HpyCH4V TGCA 3 cut(s) 10, 730, 772
HpyF10VI GCNNNNNNNGC 5 cut(s) 204, 541, 695, 934, 968
HpyF3I CTNAG 3 cut(s) 864, 929, 990
HpySE526I ACGT 1 cut(s) 798
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 3 cut(s) 197, 293, 655
HspAI GCGC 4 cut(s) 270, 432, 583, 966
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
LmnI GCTCC 5 cut(s) 494, 541, 628, 814, 983
LpnPI CCDG 9 cut(s) 217, 226, 268, 280, 336, 716, 876, 896, 919
LweI GCATC 2 cut(s) 19, 883
MaeI CTAG 2 cut(s) 425, 657
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 1 cut(s) 147
MalI GATC 7 cut(s) 279, 396, 604, 726, 851, 857, 909
MboI GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
MboII GAAGA 4 cut(s) 124, 235, 352, 649
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 4 cut(s) 394, 602, 724, 855
MhlI GDGCHC 1 cut(s) 774
MluCI AATT 4 cut(s) 15, 65, 273, 518
MlyI GAGTC 2 cut(s) 300, 952
MmeI TCCRAC 3 cut(s) 45, 185, 519
MspI CCGG 2 cut(s) 204, 255
MunI CAATTG 1 cut(s) 273
MvnI CGCG 6 cut(s) 120, 434, 563, 585, 600, 966
MwoI GCNNNNNNNGC 5 cut(s) 204, 541, 695, 934, 968
NcoI CCATGG 2 cut(s) 289, 651
NdeII GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
NheI GCTAGC 1 cut(s) 656
NlaIII CATG 3 cut(s) 197, 293, 655
NlaIV GGNNCC 3 cut(s) 221, 630, 756
NmuCI GTSAC 1 cut(s) 147
NruI TCGCGA 1 cut(s) 563
NsbI TGCGCA 1 cut(s) 271
PfeI GAWTC 7 cut(s) 209, 294, 419, 507, 565, 709, 890
PkrI GCNGC 4 cut(s) 431, 691, 970, 988
PleI GAGTC 2 cut(s) 300, 951
PpsI GAGTC 2 cut(s) 300, 951
PspEI GGTNACC 1 cut(s) 147
PspN4I GGNNCC 3 cut(s) 221, 630, 756
PspPI GGNCC 1 cut(s) 687
PstI CTGCAG 1 cut(s) 732
PsuI RGATCY 4 cut(s) 394, 602, 724, 855
RruI TCGCGA 1 cut(s) 563
SatI GCNGC 4 cut(s) 430, 690, 969, 987
Sau3AI GATC 7 cut(s) 277, 394, 602, 724, 849, 855, 907
Sau96I GGNCC 1 cut(s) 687
SchI GAGTC 2 cut(s) 300, 952
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 2 cut(s) 19, 883
SfcI CTRYAG 1 cut(s) 728
SgrAI CRCCGGYG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 4 cut(s) 15, 65, 273, 518
SspMI CTAG 2 cut(s) 425, 657
StyI CCWWGG 5 cut(s) 283, 289, 424, 651, 662
TaaI ACNGT 4 cut(s) 350, 390, 673, 920
TaiI ACGT 1 cut(s) 801
TaqI TCGA 4 cut(s) 342, 363, 906, 946
TasI AATT 4 cut(s) 15, 65, 273, 518
TauI GCSGC 3 cut(s) 432, 692, 971
TfiI GAWTC 7 cut(s) 209, 294, 419, 507, 565, 709, 890
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 1 cut(s) 986
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 5 cut(s) 45, 94, 182, 250, 809
TspGWI ACGGA 3 cut(s) 73, 362, 507
VneI GTGCAC 1 cut(s) 770
XapI RAATTY 2 cut(s) 65, 518
XmaJI CCTAGG 1 cut(s) 424
XspI CTAG 2 cut(s) 425, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.