pycom01g13570

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014337
Physical Location & Seq
Forward (+)
13998801 .. 13999790
990 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13570.1

Sequence Viewer

Length: 990 bp
ATGGCGCACCCGATTGCGGAGGCGAACGAGCAAAGCCCCTTCGGCGCGTTAACTCCGGAGGCCTTCTATTCCCGCCACAACGTCTCACACGCCTCCGAGTACACCACAAACCCCCGAGGCCTCAAGCTCTTCACCCAGTGGTGGACCCCGCTGCCTCCGACCGAGATCGTCGGGACCCTTGCCATCGTCCACGGCTACACCGGCGAGACCAGCTGGTTCGTCCAGCTCACCGCCGTCCACTTCGCCAAGTCCGGCTTCGCCGTCTGCGCCATCGACCACCAGGGCCATGGATTCTCTGATGGACTCGTCGCTCACATCCCCGACATCAACCTCGTAGTTGACGACTGCATCTCTTTCTTCGACTCGTTTCGTGCCAACCACGCGCCGAAGAACCGCCCCTCGTTCCTATACTCTGAGTCCCTCGGCGGCGCGATCGCCCTTCTCATCACGCTCCGGCAAGGAAGCGGCGCGTGGGACGGTCTCATCCTCAACGGCGCCATGTGCGGGATCAGCCCCAAGTTCAAGCCGCCGTGGCCGCTCGAGCACCTGCTCTTCCTCGTGGCCGCGGTCATCCCGACGTGGCGGGTGGTTCCCACGCGTGGGTCGCTCCCGGACGTGTCGTTCAAGGAGGAGTGGAAGCGGAAGCTGGCGCTGGCGAGTCCGAGGAGGTCGGTGGCGAGGCCACGCGCTGCGACGGCGCTTGAGCTGCTGAGAGTGTGCAAGGAGCTGCAGGGGAAATTCGAGGAGGTGGAGGTGCCGCTGCTGATAGTCCATGGCGGCGATGACGTTGTCTGCGACCCCGCGTGCGCGGAGGAGCTGTACAGGCGCGCGTCGAGCAAGGACAAAACGCTCAGGATCCATGAGGGCCTGTGGCACCAGCTGGTTGGCGAGTCCCAGGAGGATGTGGATTTGGTATTCGGTGAAATGGTGGAGTGGCTTCGTACTCGAGCTGAACGCGCCGCCGCCGCGAATGGAGGTGGTGGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

330

Amino Acids

36.11

Weight (kDa)

6.11

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 57 - 298 1.8e-57 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 62 - 297 2.1e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14980 AT5G19290
fragaria_vesca FvH4_7g19610
malus_domestica MD01G1108700.v1.1 MD07G1174800.v1.1
prunus_persica Prupe.2G213900_v2.0.a1
pyrus_communis pycom01g13570 pycom07g16920
rosa_chinensis RchiOBHm_Chr1g0363421
rosa_laevigata RLG00000027569
rosa_multiflora Rmu_sc0000339.1_g000010 Rmu_sc0004458.1_g000006
rosa_rugosa Rorug01G0306600
rosa_samantha Rh1AG315700 Rh1CG295400 Rh1DG310100
rosa_wichuraiana Rw1G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 555
AccB1I GGYRCC 4 cut(s) 494, 754, 873, 983
AccBSI CCGCTC 1 cut(s) 538
AccIII TCCGGA 1 cut(s) 55
AclWI GGATC 3 cut(s) 515, 850, 863
AcoI YGGCCR 2 cut(s) 533, 561
AcsI RAATTY 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 495
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 3 cut(s) 101, 821, 943
AfiI CCNNNNNNNGG 5 cut(s) 16, 141, 504, 599, 600
AflIII ACRYGT 2 cut(s) 596, 615
AgsI TTSAA 2 cut(s) 523, 625
AjiI CACGTC 2 cut(s) 579, 616
AjnI CCWGG 2 cut(s) 279, 894
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
AluBI AGCT 9 cut(s) 127, 213, 226, 646, 706, 727, 817, 880, 950
AluI AGCT 9 cut(s) 127, 213, 226, 646, 706, 727, 817, 880, 950
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 3 cut(s) 88, 200, 485
AlwI GGATC 3 cut(s) 515, 850, 863
Ama87I CYCGRG 3 cut(s) 114, 539, 945
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 7 cut(s) 60, 118, 283, 533, 561, 680, 865
ApeKI GCWGC 5 cut(s) 151, 689, 706, 727, 760
ApoI RAATTY 1 cut(s) 737
AsiSI GCGATCGC 1 cut(s) 435
AspS9I GGNCC 4 cut(s) 144, 174, 283, 865
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 3 cut(s) 124, 220, 932
AvaI CYCGRG 3 cut(s) 114, 539, 945
AvaII GGWCC 2 cut(s) 144, 174
BamHI GGATCC 1 cut(s) 855
BanI GGYRCC 4 cut(s) 494, 754, 873, 983
BauI CACGAG 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 5 cut(s) 138, 676, 693, 714, 747
BccI CCATC 3 cut(s) 191, 278, 293
BceAI ACGGC 6 cut(s) 208, 218, 245, 508, 514, 711
BciT130I CCWGG 2 cut(s) 281, 896
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 3 cut(s) 88, 200, 485
BfaI CTAG 1 cut(s) 988
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 3 cut(s) 498, 653, 701
BfuAI ACCTGC 1 cut(s) 555
BglI GCCNNNNNGGC 2 cut(s) 42, 532
Bme1390I CCNGG 3 cut(s) 281, 611, 896
Bme18I GGWCC 2 cut(s) 144, 174
BmeT110I CYCGRG 3 cut(s) 114, 539, 945
BmgBI CACGTC 2 cut(s) 579, 616
BmgT120I GGNCC 4 cut(s) 144, 174, 283, 865
BmiI GGNNCC 9 cut(s) 146, 175, 176, 496, 591, 756, 857, 875, 985
BmrFI CCNGG 3 cut(s) 281, 611, 896
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 1 cut(s) 357
BmuI ACTGGG 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 851
BpuEI CTTGAG 2 cut(s) 107, 722
BpuMI CCSGG 1 cut(s) 611
BsaHI GRCGYC 1 cut(s) 495
BsaI GGTCTC 2 cut(s) 200, 485
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 141, 504, 599, 600
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 136
BseAI TCCGGA 1 cut(s) 55
BseBI CCWGG 2 cut(s) 281, 896
BseGI GGATG 4 cut(s) 315, 483, 570, 907
BseLI CCNNNNNNNGG 5 cut(s) 16, 141, 504, 599, 600
BseMII CTCAG 3 cut(s) 405, 701, 865
BseNI ACTGG 1 cut(s) 136
BsePI GCGCGC 1 cut(s) 826
BseRI GAGGAG 4 cut(s) 644, 679, 758, 827
BseXI GCAGC 5 cut(s) 138, 676, 693, 714, 747
Bsh1285I CGRYCG 2 cut(s) 162, 435
BshFI GGCC 7 cut(s) 62, 120, 285, 535, 563, 682, 867
BshNI GGYRCC 4 cut(s) 494, 754, 873, 983
BsiEI CGRYCG 2 cut(s) 162, 435
BsiHKAI GWGCWC 1 cut(s) 546
BsiHKCI CYCGRG 3 cut(s) 114, 539, 945
BsiSI CCGG 5 cut(s) 56, 201, 252, 454, 611
BslFI GGGAC 4 cut(s) 187, 403, 488, 877
BslI CCNNNNNNNGG 5 cut(s) 16, 141, 504, 599, 600
BsmAI GTCTC 3 cut(s) 88, 200, 485
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 4 cut(s) 187, 403, 488, 877
BsnI GGCC 7 cut(s) 62, 120, 285, 535, 563, 682, 867
Bso31I GGTCTC 2 cut(s) 200, 485
BsoBI CYCGRG 3 cut(s) 114, 539, 945
Bsp1286I GDGCHC 1 cut(s) 546
Bsp13I TCCGGA 1 cut(s) 55
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 4 cut(s) 165, 432, 507, 855
Bsp19I CCATGG 2 cut(s) 286, 772
BspANI GGCC 7 cut(s) 62, 120, 285, 535, 563, 682, 867
BspCNI CTCAG 3 cut(s) 406, 702, 864
BspEI TCCGGA 1 cut(s) 55
BspLI GGNNCC 9 cut(s) 146, 175, 176, 496, 591, 756, 857, 875, 985
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 555
BspPI GGATC 3 cut(s) 515, 850, 863
BspQI GCTCTTC 2 cut(s) 134, 557
BspT107I GGYRCC 4 cut(s) 494, 754, 873, 983
BspTNI GGTCTC 2 cut(s) 200, 485
BsrBI CCGCTC 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 200
BssHII GCGCGC 1 cut(s) 826
BssMI GATC 4 cut(s) 165, 432, 507, 855
BssNI GRCGYC 1 cut(s) 495
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 2 cut(s) 286, 772
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 2 cut(s) 281, 896
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 2 cut(s) 134, 557
BstACI GRCGYC 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 819
BstC8I GCNNGC 4 cut(s) 648, 654, 805, 828
BstDEI CTNAG 3 cut(s) 414, 710, 851
BstDSI CCRYGG 5 cut(s) 190, 286, 530, 564, 772
BstF5I GGATG 4 cut(s) 315, 483, 570, 907
BstH2I RGCGCY 3 cut(s) 498, 653, 701
BstKTI GATC 4 cut(s) 168, 435, 510, 858
BstMAI GTCTC 3 cut(s) 88, 200, 485
BstMBI GATC 4 cut(s) 165, 432, 507, 855
BstMCI CGRYCG 2 cut(s) 162, 435
BstNI CCWGG 2 cut(s) 281, 896
BstSCI CCNGG 3 cut(s) 279, 609, 894
BstSFI CTRYAG 1 cut(s) 728
BstV1I GCAGC 5 cut(s) 138, 676, 693, 714, 747
BstX2I RGATCY 1 cut(s) 855
BstXI CCANNNNNNTGG 2 cut(s) 287, 884
BstYI RGATCY 1 cut(s) 855
BsuRI GGCC 7 cut(s) 62, 120, 285, 535, 563, 682, 867
BtgI CCRYGG 5 cut(s) 190, 286, 530, 564, 772
BtgZI GCGATG 1 cut(s) 795
BtrI CACGTC 2 cut(s) 579, 616
BtsCI GGATG 4 cut(s) 315, 483, 570, 907
BtsIMutI CAGTG 1 cut(s) 143
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 4 cut(s) 648, 654, 805, 828
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 4 cut(s) 144, 174, 283, 865
Cfr42I CCGCGG 1 cut(s) 567
CseI GACGC 1 cut(s) 819
Csp6I GTAC 3 cut(s) 100, 820, 942
CviAII CATG 4 cut(s) 287, 499, 773, 860
CviQI GTAC 3 cut(s) 100, 820, 942
DdeI CTNAG 3 cut(s) 414, 710, 851
DinI GGCGCC 1 cut(s) 496
DpnI GATC 4 cut(s) 167, 434, 509, 857
DpnII GATC 4 cut(s) 165, 432, 507, 855
DraIII CACNNNGTG 1 cut(s) 138
EaeI YGGCCR 2 cut(s) 533, 561
Eam1104I CTCTTC 2 cut(s) 134, 557
EarI CTCTTC 2 cut(s) 134, 557
Eco130I CCWWGG 2 cut(s) 286, 772
Eco147I AGGCCT 2 cut(s) 62, 120
Eco31I GGTCTC 2 cut(s) 200, 485
Eco47I GGWCC 2 cut(s) 144, 174
Eco88I CYCGRG 3 cut(s) 114, 539, 945
EcoO109I RGGNCCY 2 cut(s) 174, 865
EcoRII CCWGG 2 cut(s) 279, 894
EcoT14I CCWWGG 2 cut(s) 286, 772
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
ErhI CCWWGG 2 cut(s) 286, 772
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 4 cut(s) 290, 502, 776, 863
FaiI YATR 5 cut(s) 288, 409, 500, 774, 861
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 4 cut(s) 187, 403, 488, 877
FatI CATG 4 cut(s) 286, 498, 772, 859
FauI CCCGC 5 cut(s) 80, 156, 497, 576, 808
FokI GGATG 4 cut(s) 302, 470, 557, 914
FspBI CTAG 1 cut(s) 988
HaeII RGCGCY 3 cut(s) 498, 653, 701
HaeIII GGCC 7 cut(s) 62, 120, 285, 535, 563, 682, 867
HapII CCGG 5 cut(s) 56, 201, 252, 454, 611
HgaI GACGC 1 cut(s) 819
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 4 cut(s) 290, 502, 776, 863
HincII GTYRAC 2 cut(s) 51, 340
HindII GTYRAC 2 cut(s) 51, 340
HinfI GANTC 6 cut(s) 291, 303, 362, 416, 658, 890
HpaI GTTAAC 1 cut(s) 51
HpaII CCGG 5 cut(s) 56, 201, 252, 454, 611
HphI GGTGA 3 cut(s) 124, 220, 932
Hpy166II GTNNAC 6 cut(s) 51, 102, 144, 190, 238, 340
Hpy188I TCNGA 5 cut(s) 97, 159, 298, 415, 663
Hpy188III TCNNGA 4 cut(s) 56, 172, 574, 853
Hpy8I GTNNAC 6 cut(s) 51, 102, 144, 190, 238, 340
Hpy99I CGWCG 5 cut(s) 173, 311, 580, 697, 835
HpyAV CCTTC 3 cut(s) 49, 73, 449
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4IV ACGT 4 cut(s) 81, 578, 615, 786
HpyCH4V TGCA 3 cut(s) 348, 720, 730
HpyF3I CTNAG 3 cut(s) 414, 710, 851
HpySE526I ACGT 4 cut(s) 81, 578, 615, 786
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 4 cut(s) 290, 502, 776, 863
KasI GGCGCC 1 cut(s) 494
KflI GGGWCCC 1 cut(s) 174
Kpn2I TCCGGA 1 cut(s) 55
KspAI GTTAAC 1 cut(s) 51
KspI CCGCGG 1 cut(s) 567
Kzo9I GATC 4 cut(s) 165, 432, 507, 855
LguI GCTCTTC 2 cut(s) 134, 557
LmnI GCTCC 4 cut(s) 456, 612, 724, 814
Lsp1109I GCAGC 5 cut(s) 138, 676, 693, 714, 747
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 988
MaeII ACGT 4 cut(s) 81, 578, 615, 786
MalI GATC 4 cut(s) 167, 434, 509, 857
MbiI CCGCTC 1 cut(s) 538
MboI GATC 4 cut(s) 165, 432, 507, 855
MboII GAAGA 4 cut(s) 121, 349, 400, 544
MflI RGATCY 1 cut(s) 855
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 1 cut(s) 737
MluI ACGCGT 1 cut(s) 596
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 5 cut(s) 297, 356, 425, 667, 899
MmeI TCCRAC 1 cut(s) 182
MroI TCCGGA 1 cut(s) 55
MseI TTAA 1 cut(s) 50
MspA1I CMGCKG 5 cut(s) 151, 213, 566, 760, 880
MspI CCGG 5 cut(s) 56, 201, 252, 454, 611
MspR9I CCNGG 3 cut(s) 281, 611, 896
MvaI CCWGG 2 cut(s) 281, 896
NarI GGCGCC 1 cut(s) 495
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 2 cut(s) 286, 772
NdeII GATC 4 cut(s) 165, 432, 507, 855
NlaIII CATG 4 cut(s) 290, 502, 776, 863
NlaIV GGNNCC 9 cut(s) 146, 175, 176, 496, 591, 756, 857, 875, 985
NmeAIII GCCGAG 1 cut(s) 402
PaeR7I CTCGAG 2 cut(s) 539, 945
PaqCI CACCTGC 1 cut(s) 555
PauI GCGCGC 1 cut(s) 826
PceI AGGCCT 2 cut(s) 62, 120
PciSI GCTCTTC 2 cut(s) 134, 557
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 792
PfeI GAWTC 1 cut(s) 291
PflFI GACNNNGTC 1 cut(s) 788
PfoI TCCNGGA 1 cut(s) 609
Ple19I CGATCG 1 cut(s) 435
PleI GAGTC 5 cut(s) 297, 356, 424, 666, 898
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 5 cut(s) 297, 356, 424, 666, 898
PpuMI RGGWCCY 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 279, 894
PspGI CCWGG 2 cut(s) 279, 894
PspN4I GGNNCC 9 cut(s) 146, 175, 176, 496, 591, 756, 857, 875, 985
PspPI GGNCC 4 cut(s) 144, 174, 283, 865
PspPPI RGGWCCY 1 cut(s) 174
PspXI VCTCGAGB 2 cut(s) 539, 945
PstI CTGCAG 1 cut(s) 732
PsuI RGATCY 1 cut(s) 855
PsyI GACNNNGTC 1 cut(s) 788
PteI GCGCGC 1 cut(s) 826
PvuI CGATCG 1 cut(s) 435
PvuII CAGCTG 2 cut(s) 213, 880
RgaI GCGATCGC 1 cut(s) 435
RsaI GTAC 3 cut(s) 101, 821, 943
RsaNI GTAC 3 cut(s) 100, 820, 942
SacII CCGCGG 1 cut(s) 567
SapI GCTCTTC 2 cut(s) 134, 557
SaqAI TTAA 1 cut(s) 50
Sau3AI GATC 4 cut(s) 165, 432, 507, 855
Sau96I GGNCC 4 cut(s) 144, 174, 283, 865
SchI GAGTC 5 cut(s) 297, 356, 425, 667, 899
ScrFI CCNGG 3 cut(s) 281, 611, 896
SduI GDGCHC 1 cut(s) 546
SfaAI GCGATCGC 1 cut(s) 435
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 728
SfoI GGCGCC 1 cut(s) 496
Sfr274I CTCGAG 2 cut(s) 539, 945
Sfr303I CCGCGG 1 cut(s) 567
SgfI GCGATCGC 1 cut(s) 435
SgrAI CRCCGGYG 1 cut(s) 200
SgrBI CCGCGG 1 cut(s) 567
SinI GGWCC 2 cut(s) 144, 174
SlaI CTCGAG 2 cut(s) 539, 945
SmlI CTYRAG 4 cut(s) 122, 539, 701, 945
SmoI CTYRAG 4 cut(s) 122, 539, 701, 945
Sse9I AATT 1 cut(s) 737
SseBI AGGCCT 2 cut(s) 62, 120
SspDI GGCGCC 1 cut(s) 494
SspMI CTAG 1 cut(s) 988
StuI AGGCCT 2 cut(s) 62, 120
StyD4I CCNGG 3 cut(s) 279, 609, 894
StyI CCWWGG 2 cut(s) 286, 772
TaaI ACNGT 1 cut(s) 479
TaiI ACGT 4 cut(s) 84, 581, 618, 789
TaqI TCGA 6 cut(s) 273, 360, 540, 741, 833, 946
TaqII GACCGA 1 cut(s) 176
TasI AATT 1 cut(s) 737
TatI WGTACW 2 cut(s) 99, 819
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 1 cut(s) 143
TseI GCWGC 5 cut(s) 151, 689, 706, 727, 760
TspRI CASTG 1 cut(s) 143
Tth111I GACNNNGTC 1 cut(s) 788
VpaK11BI GGWCC 2 cut(s) 144, 174
XapI RAATTY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XhoI CTCGAG 2 cut(s) 539, 945
XspI CTAG 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.