FvH4_7g19610

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014337
Physical Location & Seq
Forward (+)
16116184 .. 16117856
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19610.t1

Sequence Viewer

Length: 987 bp
ATGCCGCACCCGATTTCGGAGGCGAACGAGAAGAGCCCCTTCGGCGACTTAACCGCCGAAGAATTCTACTCCCGCCACTCCGTCTCTCACGGCTCCGAATACGTCACCAACCCCAGAGGACTCAAGCTCTTCACCCAGTGGTGGACCCCGCTCCCCCCGGCGGAGGTCCTTGGCGTCCTCGCCATCGTCCACGGCTTCACCGGCGAGACCAGCTGGTTCGTCCAGCTCACCGCCGTCAACTTCGCCAAGGCCGGCTTCGCCGTCTGCGCCATCGACCACCAGGGCCACGGCTTCTCCGACGGCCTCGTCGCCCACATCCCCGACATCAACCCCGTCGTCGACGACTGCATTTCCTTCTTCGAGTCCTTCCGTGCCCGCCACTCGCCGTCGCTTCCTTCCTTTCTGTACGCCGAGTCCCTCGGCGGCGCAATCGCGATTCTCGTCACGCTCCGCCGCGTGGGGCGGCCCTGGGACGGACTCATCCTCAACGGCGCCATGTGCGGGATCAGCGCCAAGTTCAAGCCGCCGTGGCCGCTCGAGCACCTCCTCTTCCTCGTCGCCGCCGTCATCCCGACGTGGCGCGTCGTCCCCACGCGCGGGTCGCTGCCGGAGGTTTCGTTCAAGGAGGAGTGGAAGCGGAAGCTGGCGCTGGCCAGCCCGAGGAGGCCGCTCGCCAGGCCACGCGCCGCCACGGCGCTTGAGCTGCTTAGAGTCTGCAAGGAGCTGCAGGGGAAGTTTGAGGAGGTGGAGGTGCCGCTGCTGATCGTCCACGGCGGCGACGACGTCGTCTGCGACCCCGCGTGCGTTGAGGAGCTATTCAAGCGCGCGTCGAGCAAGGACAAGACTCTGAGGATCCACCCCGGCATGTGGCACCAGCTAATCGGCGAGCCTCCGGAGAACGTGGAGTTGGTTTTCGGAGAAATGGTGGACTGGCTTCGTACCAGAGCTGAACGCGCCTCACGCGCCTCCGTTGATGGTGGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

329

Amino Acids

36.24

Weight (kDa)

6.32

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 56 - 297 3.6e-57 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 60 - 165 8e-06 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 62 - 293 4.1e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14980 AT5G19290
fragaria_vesca FvH4_7g19610
malus_domestica MD01G1108700.v1.1 MD07G1174800.v1.1
prunus_persica Prupe.2G213900_v2.0.a1
pyrus_communis pycom01g13570 pycom07g16920
rosa_chinensis RchiOBHm_Chr1g0363421
rosa_laevigata RLG00000027569
rosa_multiflora Rmu_sc0000339.1_g000010 Rmu_sc0004458.1_g000006
rosa_rugosa Rorug01G0306600
rosa_samantha Rh1AG315700 Rh1CG295400 Rh1DG310100
rosa_wichuraiana Rw1G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 305, 785
AatII GACGTC 1 cut(s) 786
AccB1I GGYRCC 4 cut(s) 491, 751, 870, 980
AccBSI CCGCTC 3 cut(s) 151, 535, 670
AccI GTMKAC 1 cut(s) 339
AccIII TCCGGA 1 cut(s) 892
AclWI GGATC 3 cut(s) 512, 847, 860
AcoI YGGCCR 2 cut(s) 530, 651
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 3 cut(s) 174, 492, 783
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 2 cut(s) 407, 940
AgsI TTSAA 3 cut(s) 520, 622, 820
AjiI CACGTC 1 cut(s) 576
AjnI CCWGG 3 cut(s) 279, 467, 674
AjuI GAANNNNNNNTTGG 4 cut(s) 239, 271, 890, 922
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 9 cut(s) 127, 213, 226, 643, 703, 724, 814, 877, 947
AluI AGCT 9 cut(s) 127, 213, 226, 643, 703, 724, 814, 877, 947
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 2 cut(s) 88, 200
AlwI GGATC 3 cut(s) 512, 847, 860
Ama87I CYCGRG 2 cut(s) 536, 658
Aor13HI TCCGGA 1 cut(s) 892
AoxI GGCC 8 cut(s) 249, 283, 301, 464, 530, 651, 665, 677
ApeKI GCWGC 4 cut(s) 604, 703, 724, 757
ApoI RAATTY 1 cut(s) 62
AspS9I GGNCC 4 cut(s) 144, 166, 283, 465
AsuC2I CCSGG 2 cut(s) 158, 861
AsuHPI GGTGA 4 cut(s) 97, 124, 190, 220
AvaI CYCGRG 2 cut(s) 536, 658
AvaII GGWCC 2 cut(s) 144, 166
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 653
BamHI GGATCC 1 cut(s) 852
BanI GGYRCC 4 cut(s) 491, 751, 870, 980
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 4 cut(s) 591, 690, 711, 744
BccI CCATC 3 cut(s) 191, 278, 968
BciT130I CCWGG 3 cut(s) 281, 469, 676
BcnI CCSGG 2 cut(s) 158, 861
BcoDI GTCTC 2 cut(s) 88, 200
BfaI CTAG 1 cut(s) 985
BfmI CTRYAG 1 cut(s) 725
BfoI RGCGCY 4 cut(s) 495, 513, 650, 698
BglI GCCNNNNNGGC 3 cut(s) 42, 529, 692
Bme1390I CCNGG 5 cut(s) 158, 281, 469, 676, 861
Bme18I GGWCC 2 cut(s) 144, 166
BmeT110I CYCGRG 2 cut(s) 536, 658
BmgBI CACGTC 1 cut(s) 576
BmgT120I GGNCC 4 cut(s) 144, 166, 283, 465
BmiI GGNNCC 7 cut(s) 94, 146, 493, 753, 854, 872, 982
BmrFI CCNGG 5 cut(s) 158, 281, 469, 676, 861
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BpuEI CTTGAG 2 cut(s) 107, 719
BpuMI CCSGG 2 cut(s) 158, 861
BsaHI GRCGYC 3 cut(s) 174, 492, 783
BsaI GGTCTC 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 892
BsaXI ACNNNNNCTCC 6 cut(s) 278, 308, 602, 632, 909, 939
Bse118I RCCGGY 2 cut(s) 200, 251
Bse1I ACTGG 2 cut(s) 136, 935
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 3 cut(s) 281, 469, 676
BseGI GGATG 3 cut(s) 315, 480, 567
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 2 cut(s) 136, 935
BsePI GCGCGC 1 cut(s) 823
BseRI GAGGAG 5 cut(s) 536, 641, 676, 755, 824
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 4 cut(s) 591, 690, 711, 744
BshFI GGCC 8 cut(s) 251, 285, 303, 466, 532, 653, 667, 679
BshNI GGYRCC 4 cut(s) 491, 751, 870, 980
BsiHKAI GWGCWC 1 cut(s) 543
BsiHKCI CYCGRG 2 cut(s) 536, 658
BsiSI CCGG 6 cut(s) 158, 201, 252, 608, 861, 893
BslFI GGGAC 3 cut(s) 400, 485, 572
BsmAI GTCTC 2 cut(s) 88, 200
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 3 cut(s) 400, 485, 572
BsnI GGCC 8 cut(s) 251, 285, 303, 466, 532, 653, 667, 679
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 2 cut(s) 536, 658
Bsp1286I GDGCHC 3 cut(s) 38, 376, 543
Bsp13I TCCGGA 1 cut(s) 892
Bsp143I GATC 3 cut(s) 504, 762, 852
Bsp68I TCGCGA 1 cut(s) 434
BspANI GGCC 8 cut(s) 251, 285, 303, 466, 532, 653, 667, 679
BspCNI CTCAG 1 cut(s) 840
BspEI TCCGGA 1 cut(s) 892
BspLI GGNNCC 7 cut(s) 94, 146, 493, 753, 854, 872, 982
BspMAI CTGCAG 1 cut(s) 729
BspPI GGATC 3 cut(s) 512, 847, 860
BspQI GCTCTTC 2 cut(s) 26, 134
BspT107I GGYRCC 4 cut(s) 491, 751, 870, 980
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 3 cut(s) 151, 535, 670
BsrFI RCCGGY 2 cut(s) 200, 251
BsrI ACTGG 2 cut(s) 136, 935
BssAI RCCGGY 2 cut(s) 200, 251
BssHII GCGCGC 1 cut(s) 823
BssMI GATC 3 cut(s) 504, 762, 852
BssNI GRCGYC 3 cut(s) 174, 492, 783
BssT1I CCWWGG 2 cut(s) 169, 246
Bst2UI CCWGG 3 cut(s) 281, 469, 676
Bst6I CTCTTC 3 cut(s) 26, 134, 554
BstACI GRCGYC 3 cut(s) 174, 492, 783
BstC8I GCNNGC 9 cut(s) 253, 376, 645, 651, 655, 672, 802, 825, 887
BstDEI CTNAG 2 cut(s) 707, 848
BstDSI CCRYGG 5 cut(s) 190, 286, 527, 690, 769
BstF5I GGATG 3 cut(s) 315, 480, 567
BstH2I RGCGCY 4 cut(s) 495, 513, 650, 698
BstKTI GATC 3 cut(s) 507, 765, 855
BstMAI GTCTC 2 cut(s) 88, 200
BstMBI GATC 3 cut(s) 504, 762, 852
BstNI CCWGG 3 cut(s) 281, 469, 676
BstNSI RCATGY 1 cut(s) 868
BstSCI CCNGG 5 cut(s) 156, 279, 467, 674, 859
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 4 cut(s) 591, 690, 711, 744
BstX2I RGATCY 1 cut(s) 852
BstYI RGATCY 1 cut(s) 852
BsuRI GGCC 8 cut(s) 251, 285, 303, 466, 532, 653, 667, 679
BtgI CCRYGG 5 cut(s) 190, 286, 527, 690, 769
BtrI CACGTC 1 cut(s) 576
BtsCI GGATG 3 cut(s) 315, 480, 567
BtsIMutI CAGTG 1 cut(s) 143
BtuMI TCGCGA 1 cut(s) 434
Cac8I GCNNGC 9 cut(s) 253, 376, 645, 651, 655, 672, 802, 825, 887
Cfr10I RCCGGY 2 cut(s) 200, 251
Cfr13I GGNCC 4 cut(s) 144, 166, 283, 465
CseI GACGC 3 cut(s) 163, 571, 816
Csp6I GTAC 2 cut(s) 406, 939
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviAII CATG 2 cut(s) 496, 865
CviQI GTAC 2 cut(s) 406, 939
DdeI CTNAG 2 cut(s) 707, 848
DinI GGCGCC 1 cut(s) 493
DpnI GATC 3 cut(s) 506, 764, 854
DpnII GATC 3 cut(s) 504, 762, 852
DraIII CACNNNGTG 1 cut(s) 138
DrdI GACNNNNNNGTC 2 cut(s) 305, 785
DseDI GACNNNNNNGTC 2 cut(s) 305, 785
EaeI YGGCCR 2 cut(s) 530, 651
Eam1104I CTCTTC 3 cut(s) 26, 134, 554
EarI CTCTTC 3 cut(s) 26, 134, 554
EciI GGCGGA 2 cut(s) 176, 440
Eco130I CCWWGG 2 cut(s) 169, 246
Eco24I GRGCYC 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 2 cut(s) 144, 166
Eco88I CYCGRG 2 cut(s) 536, 658
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 279, 467, 674
EcoT14I CCWWGG 2 cut(s) 169, 246
EcoT38I GRGCYC 1 cut(s) 38
EgeI GGCGCC 1 cut(s) 493
EheI GGCGCC 1 cut(s) 493
ErhI CCWWGG 2 cut(s) 169, 246
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 2 cut(s) 499, 868
FaiI YATR 2 cut(s) 497, 866
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 239, 271
FaqI GGGAC 3 cut(s) 400, 485, 572
FatI CATG 2 cut(s) 495, 864
FauI CCCGC 6 cut(s) 80, 156, 383, 494, 590, 805
FblI GTMKAC 1 cut(s) 339
FokI GGATG 3 cut(s) 302, 467, 554
FriOI GRGCYC 1 cut(s) 38
FspBI CTAG 1 cut(s) 985
HaeII RGCGCY 4 cut(s) 495, 513, 650, 698
HaeIII GGCC 8 cut(s) 251, 285, 303, 466, 532, 653, 667, 679
HapII CCGG 6 cut(s) 158, 201, 252, 608, 861, 893
HgaI GACGC 3 cut(s) 163, 571, 816
Hin1I GRCGYC 3 cut(s) 174, 492, 783
Hin1II CATG 2 cut(s) 499, 868
HincII GTYRAC 2 cut(s) 238, 340
HindII GTYRAC 2 cut(s) 238, 340
HinfI GANTC 7 cut(s) 120, 362, 413, 436, 477, 711, 844
HpaII CCGG 6 cut(s) 158, 201, 252, 608, 861, 893
HphI GGTGA 4 cut(s) 97, 124, 190, 220
Hpy166II GTNNAC 6 cut(s) 144, 190, 238, 340, 769, 928
Hpy188I TCNGA 5 cut(s) 19, 97, 298, 849, 917
Hpy188III TCNNGA 3 cut(s) 433, 571, 893
Hpy8I GTNNAC 6 cut(s) 144, 190, 238, 340, 769, 928
HpyAV CCTTC 4 cut(s) 49, 364, 376, 405
HpyCH4IV ACGT 4 cut(s) 102, 575, 783, 900
HpyCH4V TGCA 3 cut(s) 348, 717, 727
HpyF3I CTNAG 2 cut(s) 707, 848
HpySE526I ACGT 4 cut(s) 102, 575, 783, 900
Hsp92I GRCGYC 3 cut(s) 174, 492, 783
Hsp92II CATG 2 cut(s) 499, 868
KasI GGCGCC 1 cut(s) 491
Kpn2I TCCGGA 1 cut(s) 892
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 3 cut(s) 504, 762, 852
LguI GCTCTTC 2 cut(s) 26, 134
LmnI GCTCC 5 cut(s) 98, 156, 453, 721, 811
Lsp1109I GCAGC 4 cut(s) 591, 690, 711, 744
MaeI CTAG 1 cut(s) 985
MaeII ACGT 4 cut(s) 102, 575, 783, 900
MaeIII GTNAC 2 cut(s) 103, 442
MalI GATC 3 cut(s) 506, 764, 854
MbiI CCGCTC 3 cut(s) 151, 535, 670
MboI GATC 3 cut(s) 504, 762, 852
MboII GAAGA 5 cut(s) 43, 71, 121, 349, 541
MflI RGATCY 1 cut(s) 852
MhlI GDGCHC 3 cut(s) 38, 376, 543
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 1 cut(s) 62
MluNI TGGCCA 1 cut(s) 653
Mly113I GGCGCC 1 cut(s) 492
MlyI GAGTC 6 cut(s) 114, 371, 422, 471, 720, 838
MmeI TCCRAC 1 cut(s) 321
Mox20I TGGCCA 1 cut(s) 653
MroI TCCGGA 1 cut(s) 892
MroNI GCCGGC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 653
MseI TTAA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 2 cut(s) 213, 757
MspI CCGG 6 cut(s) 158, 201, 252, 608, 861, 893
MspR9I CCNGG 5 cut(s) 158, 281, 469, 676, 861
MvaI CCWGG 3 cut(s) 281, 469, 676
NaeI GCCGGC 1 cut(s) 253
NarI GGCGCC 1 cut(s) 492
NciI CCSGG 2 cut(s) 158, 861
NdeII GATC 3 cut(s) 504, 762, 852
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 2 cut(s) 499, 868
NlaIV GGNNCC 7 cut(s) 94, 146, 493, 753, 854, 872, 982
NmeAIII GCCGAG 2 cut(s) 399, 436
NmuCI GTSAC 2 cut(s) 103, 442
NruI TCGCGA 1 cut(s) 434
NspI RCATGY 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 536
PasI CCCWGGG 1 cut(s) 468
PauI GCGCGC 1 cut(s) 823
PciSI GCTCTTC 2 cut(s) 26, 134
PcsI WCGNNNNNNNCGW 5 cut(s) 294, 438, 561, 777, 789
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 436
PflFI GACNNNGTC 2 cut(s) 782, 785
PleI GAGTC 6 cut(s) 114, 370, 421, 471, 719, 838
PluTI GGCGCC 1 cut(s) 495
PpsI GAGTC 6 cut(s) 114, 370, 421, 471, 719, 838
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 279, 467, 674
PspGI CCWGG 3 cut(s) 279, 467, 674
PspN4I GGNNCC 7 cut(s) 94, 146, 493, 753, 854, 872, 982
PspPI GGNCC 4 cut(s) 144, 166, 283, 465
PspPPI RGGWCCY 1 cut(s) 166
PspXI VCTCGAGB 1 cut(s) 536
PstI CTGCAG 1 cut(s) 729
PsuI RGATCY 1 cut(s) 852
PsyI GACNNNGTC 2 cut(s) 782, 785
PteI GCGCGC 1 cut(s) 823
PvuII CAGCTG 1 cut(s) 213
RruI TCGCGA 1 cut(s) 434
RsaI GTAC 2 cut(s) 407, 940
RsaNI GTAC 2 cut(s) 406, 939
SalI GTCGAC 1 cut(s) 338
SapI GCTCTTC 2 cut(s) 26, 134
SaqAI TTAA 1 cut(s) 50
Sau3AI GATC 3 cut(s) 504, 762, 852
Sau96I GGNCC 4 cut(s) 144, 166, 283, 465
SchI GAGTC 6 cut(s) 114, 371, 422, 471, 720, 838
ScrFI CCNGG 5 cut(s) 158, 281, 469, 676, 861
SduI GDGCHC 3 cut(s) 38, 376, 543
SfcI CTRYAG 1 cut(s) 725
SfoI GGCGCC 1 cut(s) 493
Sfr274I CTCGAG 1 cut(s) 536
SgrAI CRCCGGYG 1 cut(s) 200
SgrDI CGTCGACG 1 cut(s) 338
SinI GGWCC 2 cut(s) 144, 166
SlaI CTCGAG 1 cut(s) 536
SmlI CTYRAG 3 cut(s) 122, 536, 698
SmoI CTYRAG 3 cut(s) 122, 536, 698
Sse9I AATT 1 cut(s) 62
SspDI GGCGCC 1 cut(s) 491
SspMI CTAG 1 cut(s) 985
StyD4I CCNGG 5 cut(s) 156, 279, 467, 674, 859
StyI CCWWGG 2 cut(s) 169, 246
TaiI ACGT 4 cut(s) 105, 578, 786, 903
TaqI TCGA 5 cut(s) 273, 339, 360, 537, 830
TasI AATT 1 cut(s) 62
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 2 cut(s) 103, 442
TseI GCWGC 4 cut(s) 604, 703, 724, 757
Tsp45I GTSAC 2 cut(s) 103, 442
TspGWI ACGGA 4 cut(s) 70, 359, 489, 958
TspRI CASTG 1 cut(s) 143
Tth111I GACNNNGTC 2 cut(s) 782, 785
VpaK11BI GGWCC 2 cut(s) 144, 166
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 1 cut(s) 985
ZraI GACGTC 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.