MD07G1174800.v1.1

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014337
Physical Location & Seq
Forward (+)
25347166 .. 25348158
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1174800.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCGCACCCGATTGCGGAGGCGAACGAACAAAGCCCCTTCGGCGCGTTGACCCCAGAGGCATTCTATTCCCGCCACAACGTCTCTCACGCCTCCGAGTACTTCACAAACCCTAGAGGCCTCAAGCGCTTCACCCAGTGGTGGACCCCGCTCCCTCCGACCGAGATTGTAGGAACCCTCGCCATCGTCCACGGCTTCACCGGCGAGACCAGCTGGTTCGTCCAGCTCACCGCCGTCCACTTCGCCAAGTCTGGCTTCGTCGTCTGCGCCATTGATCATCAGGGTCACGGCTTCTCCGATGGCCTCGCCGCCCACATCCCCGACATCAACCCCGTCGTCGACGACTGCATCTCCTTCTTCGACTCATTCCGAGCCAATCACGCGCCGAAGAACCGCCCCTCGTTCCTCTACGCCGAGTCCCTCGGTGGCGCCATCGCACTCCTCATGACTTTCCGGCAGGGAAGCGGCGCGTGGGATGGCCTCATCCTCAATGGCGCCATGTGCGGGATCAGCCCCAAGTTTAAGCCGCCGTGGCCGCTCGAGCACCTGCTATTTCTCGTCGCCGCGGTGATACCGACGTGGCGGGTTGTCCCCACCCGCGGATCGATTACGGACGTGTCGTTCAAGGAGGAGTGGAAGCGGAAGCTGGCGCTGGCGAACCCGAGGAGGCCGGTGGCGAGGCCTCGCGCATCGACGGCTCTTGAGCTGCTGAGAGTGTGTAAGGAGCTGCAGGGGAGATTCGAGGAGATGGAGGTGCCGGTGCTGATAGTTCACGGCGGCGACGACGTCCTTTGCGACCCGGCGTGCGTGGAAGAGCTGTACAGGCGCGCGTCGAGCAAGGACAAGACGATCAGGATCCACGAGGGATTGTGGCACCAGCTGATTGGCGAGTCCCAGGAGAATGTGGACTTGGTGTTCGGAGAAATGGTGGAGTGGCTTCGCACTCGAGCTGAACGCGCCGCCGTCTCGAATGGTGGCAGCGATGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

331

Amino Acids

36.55

Weight (kDa)

6.23

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 57 - 298 3.9e-56 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 62 - 294 1.4e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14980 AT5G19290
fragaria_vesca FvH4_7g19610
malus_domestica MD01G1108700.v1.1 MD07G1174800.v1.1
prunus_persica Prupe.2G213900_v2.0.a1
pyrus_communis pycom01g13570 pycom07g16920
rosa_chinensis RchiOBHm_Chr1g0363421
rosa_laevigata RLG00000027569
rosa_multiflora Rmu_sc0000339.1_g000010 Rmu_sc0004458.1_g000006
rosa_rugosa Rorug01G0306600
rosa_samantha Rh1AG315700 Rh1CG295400 Rh1DG310100
rosa_wichuraiana Rw1G027960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 555
AatII GACGTC 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 555
AccB1I GGYRCC 4 cut(s) 428, 494, 754, 873
AccBSI CCGCTC 2 cut(s) 151, 538
AccI GTMKAC 1 cut(s) 339
AccII CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
AclWI GGATC 4 cut(s) 515, 610, 850, 863
AcoI YGGCCR 1 cut(s) 533
AcyI GRCGYC 3 cut(s) 429, 495, 786
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 2 cut(s) 101, 821
AfeI AGCGCT 1 cut(s) 128
AfiI CCNNNNNNNGG 4 cut(s) 16, 141, 504, 599
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 1 cut(s) 625
AjiI CACGTC 2 cut(s) 579, 616
AjnI CCWGG 1 cut(s) 894
AjuI GAANNNNNNNTTGG 4 cut(s) 239, 271, 893, 925
AloI GAACNNNNNNTCC 4 cut(s) 605, 637, 899, 931
AluBI AGCT 8 cut(s) 213, 226, 646, 706, 727, 817, 880, 950
AluI AGCT 8 cut(s) 213, 226, 646, 706, 727, 817, 880, 950
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 3 cut(s) 88, 200, 970
AlwI GGATC 4 cut(s) 515, 610, 850, 863
Ama87I CYCGRG 3 cut(s) 539, 661, 945
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 6 cut(s) 118, 301, 478, 533, 668, 680
ApeKI GCWGC 3 cut(s) 706, 727, 978
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AspS9I GGNCC 1 cut(s) 144
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 4 cut(s) 124, 190, 220, 580
AvaI CYCGRG 3 cut(s) 539, 661, 945
AvaII GGWCC 1 cut(s) 144
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BamHI GGATCC 1 cut(s) 855
BanI GGYRCC 4 cut(s) 428, 494, 754, 873
BauI CACGAG 1 cut(s) 860
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 2 cut(s) 693, 714
BccI CCATC 6 cut(s) 191, 293, 440, 470, 742, 977
BceAI ACGGC 7 cut(s) 208, 218, 304, 514, 711, 790, 947
BciT130I CCWGG 1 cut(s) 896
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 800
BcoDI GTCTC 3 cut(s) 88, 200, 970
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 4 cut(s) 130, 432, 498, 653
BfuAI ACCTGC 1 cut(s) 555
BglI GCCNNNNNGGC 2 cut(s) 42, 532
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 2 cut(s) 800, 896
Bme18I GGWCC 1 cut(s) 144
BmeT110I CYCGRG 3 cut(s) 539, 661, 945
BmgBI CACGTC 2 cut(s) 579, 616
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 7 cut(s) 146, 175, 430, 496, 756, 857, 875
BmrFI CCNGG 2 cut(s) 800, 896
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 2 cut(s) 357, 698
BmuI ACTGGG 1 cut(s) 130
BpuEI CTTGAG 2 cut(s) 107, 722
BpuMI CCSGG 1 cut(s) 800
Bsa29I ATCGAT 1 cut(s) 605
BsaBI GATNNNNATC 1 cut(s) 854
BsaHI GRCGYC 3 cut(s) 429, 495, 786
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 7 cut(s) 190, 421, 530, 564, 598, 662, 894
BsaXI ACNNNNNCTCC 6 cut(s) 278, 308, 335, 365, 912, 942
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 141, 504, 599
Bse118I RCCGGY 3 cut(s) 200, 670, 757
Bse1I ACTGG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 854
BseBI CCWGG 1 cut(s) 896
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 7 cut(s) 190, 421, 530, 564, 598, 662, 894
BseGI GGATG 3 cut(s) 315, 481, 483
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 4 cut(s) 16, 141, 504, 599
BseMII CTCAG 1 cut(s) 701
BseNI ACTGG 1 cut(s) 136
BsePI GCGCGC 1 cut(s) 826
BseRI GAGGAG 4 cut(s) 431, 644, 679, 758
BseXI GCAGC 2 cut(s) 693, 714
Bsh1236I CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 6 cut(s) 120, 303, 480, 535, 670, 682
BshNI GGYRCC 4 cut(s) 428, 494, 754, 873
BshVI ATCGAT 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 546
BsiHKCI CYCGRG 3 cut(s) 539, 661, 945
BsiSI CCGG 5 cut(s) 201, 454, 671, 758, 800
BslFI GGGAC 3 cut(s) 403, 575, 877
BslI CCNNNNNNNGG 4 cut(s) 16, 141, 504, 599
BsmAI GTCTC 3 cut(s) 88, 200, 970
BsmBI CGTCTC 2 cut(s) 88, 970
BsmFI GGGAC 3 cut(s) 403, 575, 877
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 6 cut(s) 120, 303, 480, 535, 670, 682
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 3 cut(s) 539, 661, 945
Bsp1286I GDGCHC 1 cut(s) 546
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 5 cut(s) 274, 507, 602, 849, 855
BspANI GGCC 6 cut(s) 120, 303, 480, 535, 670, 682
BspCNI CTCAG 1 cut(s) 702
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 7 cut(s) 146, 175, 430, 496, 756, 857, 875
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 555
BspPI GGATC 4 cut(s) 515, 610, 850, 863
BspQI GCTCTTC 1 cut(s) 807
BspT107I GGYRCC 4 cut(s) 428, 494, 754, 873
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 2 cut(s) 151, 538
BsrFI RCCGGY 3 cut(s) 200, 670, 757
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 3 cut(s) 200, 670, 757
BssECI CCNNGG 7 cut(s) 190, 421, 530, 564, 598, 662, 894
BssHII GCGCGC 1 cut(s) 826
BssMI GATC 5 cut(s) 274, 507, 602, 849, 855
BssNI GRCGYC 3 cut(s) 429, 495, 786
BssSI CACGAG 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 860
Bst2UI CCWGG 1 cut(s) 896
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 3 cut(s) 429, 495, 786
BstAUI TGTACA 1 cut(s) 819
BstC8I GCNNGC 4 cut(s) 648, 654, 805, 828
BstDEI CTNAG 2 cut(s) 710, 990
BstDSI CCRYGG 4 cut(s) 190, 530, 564, 598
BstF5I GGATG 3 cut(s) 315, 481, 483
BstFNI CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
BstH2I RGCGCY 4 cut(s) 130, 432, 498, 653
BstKTI GATC 5 cut(s) 277, 510, 605, 852, 858
BstMAI GTCTC 3 cut(s) 88, 200, 970
BstMBI GATC 5 cut(s) 274, 507, 602, 849, 855
BstMCI CGRYCG 1 cut(s) 162
BstNI CCWGG 1 cut(s) 896
BstSCI CCNGG 2 cut(s) 798, 894
BstSFI CTRYAG 1 cut(s) 728
BstUI CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
BstV1I GCAGC 2 cut(s) 693, 714
BstX2I RGATCY 1 cut(s) 855
BstXI CCANNNNNNTGG 1 cut(s) 884
BstYI RGATCY 1 cut(s) 855
Bsu15I ATCGAT 1 cut(s) 605
BsuRI GGCC 6 cut(s) 120, 303, 480, 535, 670, 682
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 4 cut(s) 190, 530, 564, 598
BtgZI GCGATG 1 cut(s) 418
BtrI CACGTC 2 cut(s) 579, 616
BtsCI GGATG 3 cut(s) 315, 481, 483
BtsIMutI CAGTG 1 cut(s) 143
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 4 cut(s) 648, 654, 805, 828
CciI TCATGA 1 cut(s) 444
Cfr10I RCCGGY 3 cut(s) 200, 670, 757
Cfr13I GGNCC 1 cut(s) 144
Cfr42I CCGCGG 2 cut(s) 567, 601
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 819
Csp6I GTAC 2 cut(s) 100, 820
CviAII CATG 2 cut(s) 445, 499
CviQI GTAC 2 cut(s) 100, 820
DdeI CTNAG 2 cut(s) 710, 990
DinI GGCGCC 2 cut(s) 430, 496
DpnI GATC 5 cut(s) 276, 509, 604, 851, 857
DpnII GATC 5 cut(s) 274, 507, 602, 849, 855
DraIII CACNNNGTG 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco147I AGGCCT 2 cut(s) 120, 682
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 144
Eco47III AGCGCT 1 cut(s) 128
Eco88I CYCGRG 3 cut(s) 539, 661, 945
EcoRII CCWGG 1 cut(s) 894
EgeI GGCGCC 2 cut(s) 430, 496
EheI GGCGCC 2 cut(s) 430, 496
Esp3I CGTCTC 2 cut(s) 88, 970
FaeI CATG 2 cut(s) 448, 502
FaiI YATR 2 cut(s) 446, 500
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 3 cut(s) 403, 575, 877
FatI CATG 2 cut(s) 444, 498
FauI CCCGC 5 cut(s) 80, 156, 497, 576, 605
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 1 cut(s) 339
FokI GGATG 3 cut(s) 302, 470, 488
FspBI CTAG 1 cut(s) 114
HaeII RGCGCY 4 cut(s) 130, 432, 498, 653
HaeIII GGCC 6 cut(s) 120, 303, 480, 535, 670, 682
HapII CCGG 5 cut(s) 201, 454, 671, 758, 800
HgaI GACGC 1 cut(s) 819
Hin1I GRCGYC 3 cut(s) 429, 495, 786
Hin1II CATG 2 cut(s) 448, 502
HincII GTYRAC 2 cut(s) 51, 340
HindII GTYRAC 2 cut(s) 51, 340
HinfI GANTC 4 cut(s) 362, 416, 738, 890
HpaII CCGG 5 cut(s) 201, 454, 671, 758, 800
HphI GGTGA 4 cut(s) 124, 190, 220, 580
Hpy166II GTNNAC 7 cut(s) 51, 144, 190, 238, 340, 772, 907
Hpy188I TCNGA 5 cut(s) 97, 159, 298, 371, 920
Hpy188III TCNNGA 4 cut(s) 445, 701, 853, 967
Hpy8I GTNNAC 7 cut(s) 51, 144, 190, 238, 340, 772, 907
HpyAV CCTTC 2 cut(s) 49, 364
HpyCH4IV ACGT 4 cut(s) 81, 578, 615, 786
HpyCH4V TGCA 2 cut(s) 348, 730
HpyF3I CTNAG 2 cut(s) 710, 990
HpySE526I ACGT 4 cut(s) 81, 578, 615, 786
Hsp92I GRCGYC 3 cut(s) 429, 495, 786
Hsp92II CATG 2 cut(s) 448, 502
KasI GGCGCC 2 cut(s) 428, 494
Ksp22I TGATCA 1 cut(s) 274
KspI CCGCGG 2 cut(s) 567, 601
Kzo9I GATC 5 cut(s) 274, 507, 602, 849, 855
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 2 cut(s) 156, 724
Lsp1109I GCAGC 2 cut(s) 693, 714
LweI GCATC 2 cut(s) 357, 698
MaeI CTAG 1 cut(s) 114
MaeII ACGT 4 cut(s) 81, 578, 615, 786
MaeIII GTNAC 1 cut(s) 284
MalI GATC 5 cut(s) 276, 509, 604, 851, 857
MbiI CCGCTC 2 cut(s) 151, 538
MboI GATC 5 cut(s) 274, 507, 602, 849, 855
MboII GAAGA 3 cut(s) 349, 400, 824
MflI RGATCY 1 cut(s) 855
MhlI GDGCHC 1 cut(s) 546
Mly113I GGCGCC 2 cut(s) 429, 495
MlyI GAGTC 3 cut(s) 356, 425, 899
MmeI TCCRAC 1 cut(s) 182
MseI TTAA 1 cut(s) 522
MspA1I CMGCKG 4 cut(s) 213, 566, 600, 880
MspI CCGG 5 cut(s) 201, 454, 671, 758, 800
MspR9I CCNGG 2 cut(s) 800, 896
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 896
MvnI CGCG 9 cut(s) 47, 383, 470, 566, 600, 687, 828, 830, 957
NarI GGCGCC 2 cut(s) 429, 495
NciI CCSGG 1 cut(s) 800
NdeII GATC 5 cut(s) 274, 507, 602, 849, 855
NlaIII CATG 2 cut(s) 448, 502
NlaIV GGNNCC 7 cut(s) 146, 175, 430, 496, 756, 857, 875
NmeAIII GCCGAG 1 cut(s) 439
NmuCI GTSAC 1 cut(s) 284
PaeR7I CTCGAG 2 cut(s) 539, 945
PagI TCATGA 1 cut(s) 444
PaqCI CACCTGC 1 cut(s) 555
PauI GCGCGC 1 cut(s) 826
PceI AGGCCT 2 cut(s) 120, 682
PciSI GCTCTTC 1 cut(s) 807
PcsI WCGNNNNNNNCGW 4 cut(s) 294, 617, 780, 792
PctI GAATGC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 738
PflFI GACNNNGTC 1 cut(s) 785
PleI GAGTC 3 cut(s) 356, 424, 898
PluTI GGCGCC 2 cut(s) 432, 498
PpsI GAGTC 3 cut(s) 356, 424, 898
Psp6I CCWGG 1 cut(s) 894
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 7 cut(s) 146, 175, 430, 496, 756, 857, 875
PspPI GGNCC 1 cut(s) 144
PspXI VCTCGAGB 2 cut(s) 539, 945
PstI CTGCAG 1 cut(s) 732
PsuI RGATCY 1 cut(s) 855
PsyI GACNNNGTC 1 cut(s) 785
PteI GCGCGC 1 cut(s) 826
PvuII CAGCTG 2 cut(s) 213, 880
RsaI GTAC 2 cut(s) 101, 821
RsaNI GTAC 2 cut(s) 100, 820
SacII CCGCGG 2 cut(s) 567, 601
SalI GTCGAC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 1 cut(s) 522
Sau3AI GATC 5 cut(s) 274, 507, 602, 849, 855
Sau96I GGNCC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 3 cut(s) 356, 425, 899
ScrFI CCNGG 2 cut(s) 800, 896
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 2 cut(s) 357, 698
SfcI CTRYAG 1 cut(s) 728
SfoI GGCGCC 2 cut(s) 430, 496
Sfr274I CTCGAG 2 cut(s) 539, 945
Sfr303I CCGCGG 2 cut(s) 567, 601
SgrAI CRCCGGYG 1 cut(s) 200
SgrBI CCGCGG 2 cut(s) 567, 601
SgrDI CGTCGACG 1 cut(s) 338
SinI GGWCC 1 cut(s) 144
SlaI CTCGAG 2 cut(s) 539, 945
SmlI CTYRAG 4 cut(s) 122, 539, 701, 945
SmoI CTYRAG 4 cut(s) 122, 539, 701, 945
SseBI AGGCCT 2 cut(s) 120, 682
SspDI GGCGCC 2 cut(s) 428, 494
SspMI CTAG 1 cut(s) 114
StuI AGGCCT 2 cut(s) 120, 682
StyD4I CCNGG 2 cut(s) 798, 894
TaiI ACGT 4 cut(s) 84, 581, 618, 789
TaqI TCGA 9 cut(s) 339, 360, 540, 605, 692, 741, 833, 946, 968
TaqII GACCGA 1 cut(s) 176
TatI WGTACW 2 cut(s) 99, 819
TauI GCSGC 7 cut(s) 311, 468, 529, 538, 566, 780, 962
TfiI GAWTC 1 cut(s) 738
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 706, 727, 978
Tsp45I GTSAC 1 cut(s) 284
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 1 cut(s) 143
Tth111I GACNNNGTC 1 cut(s) 785
VpaK11BI GGWCC 1 cut(s) 144
XhoI CTCGAG 2 cut(s) 539, 945
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 1 cut(s) 114
ZraI GACGTC 1 cut(s) 787
ZrmI AGTACT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.