AT5G20250

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009084
Physical Location & Seq
Forward (+)
6833564 .. 6836862
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20250.3

Sequence Viewer

Length: 2250 bp
ATGACGATTAAACCGGCGGTTCGTATTTCCGACGGGAATCTAATCATCAAAAACCGGACGATTCTAACCGGTGTACCAGATAATGTCATCACGACGTCAGCATCGGAAGCTGGACCGGTAGAAGGAGTCTTCGTCGGAGCTGTATTTAACAAGGAAGAGAGTAAACACATCGTACCGATCGGTACGCTTCGCAATTCCCGGTTTATGTCTTGTTTCCGGTTTAAGCTCTGGTGGATGGCCCAGAGAATGGGAGAAATGGGCCGAGATATTCCCTACGAGACGCAGTTCTTATTGGTCGAGAGCAACGATGGGTCCCACCTTGAGTCTGACGGAGCTAACGGCGTCGAGTGTAACCAGAAAGTTTACACCGTTTTCTTGCCGTTAATCGAAGGATCTTTCCGTTCGTGTCTCCAAGGAAACGTTAACGATGAGGTTGAGCTCTGTTTGGAGAGTGGTGACGTGGACACTAAACGGTCGTCGTTTACTCACTCTCTGTATATTCACGCCGGTACAGATCCGTTTCAGACAATAACGGACGCTATTCGCACCGTTAAGTTGCATCTGAATAGTTTCCGTCAACGTCACGAAAAGAAGCTTCCAGGGATCGTTGACTACTTCGGATGGTGCACTTGGGACGCGTTTTATCAAGAAGTGACTCAGGAAGGCGTCGAAGCTGGTCTTAAGTCTCTCGCCGCCGGTGGTACGCCGCCGAAGTTTGTTATCATAGACGACGGTTGGCAATCAGTTGAGAGAGATGCCACGGTGGAGGCCGGAGATGAGAAGAAAGAGTCACCGATTTTTCGACTGACGGGGATCAAGGAGAACGAAAAGTTTAAGAAGAAGGATGATCCAAACGTTGGGATTAAGAACATTGTCAAGATTGCTAAGGAGAAACACGGTTTGAAATATGTTTACGTGTGGCACGCGATAACGGGTTATTGGGGCGGAGTTAGACCCGGGGAAGAATACGGATCTGTTATGAAATATCCTAATATGTCAAAGGGTGTGGTGGAGAATGATCCGACGTGGAAGACTGATGTAATGACGCTGCAAGGGTTGGGTTTGGTTAGCCCCAAGAAAGTGTATAAGTTTTACAATGAGCTTCATAGTTACTTGGCTGATGCTGGCGTGGACGGTGTGAAAGTGGATGTGCAGTGTGTATTGGAGACTTTGGGTGGTGGTTTAGGCGGTCGGGTTGAGCTGACTCGTCAGTTTCATCAAGCTCTTGACTCCTCTGTTGCTAAGAACTTCCCGGATAATGGCTGCATTGCTTGTATGAGCCATAATACGGATGCTCTTTACTGCTCGAAGCAAGCAGCTGTGATTAGAGCATCAGATGATTTCTATCCACGGGATCCGGTGTCTCATACCATCCATATAGCCTCCGTTGCTTATAACAGTGTGTTCTTGGGAGAGTTTATGCAGCCTGACTGGGATATGTTCCATTCCGTGCACCCTGCTGCAGAGTATCATGCCTCTGCTAGGGCCATCAGTGGTGGGCCTCTCTATGTCAGTGATTCTCCTGGAAAGCACAACTTTGAGCTTCTAAGAAAGCTTGTATTGCCTGATGGATCGATTCTTCGTGCTCGACTCCCTGGTAGACCAACTCGCGATTGTTTGTTCGCTGATCCTGCCCGTGATGGTGTCAGCTTGCTAAAGATATGGAACATGAACAAGTACACTGGAGTTCTTGGCGTGTATAACTGCCAAGGAGCAGCTTGGAGCAGCACAGAGAGAAAAAACATTTTCCACCAGACTAAAACTGATAGCCTCACTGGCTCCATTCGTGGTCGTGATGTGCATTCAATATCGGAGGCCTCCACTGATCCAACAACCTGGAATGGAGACTGTGCTGTTTACTCCCAGAGCAGAGGCGAACTTATTGTTATGCCATACAATGTGTCTCTTCCAGTCTCACTCAAAATCCGTGAGCACGAGATCTTCACGGTGAGCCCCATTAGTCATCTTGTTGATGGTGTATCTTTTGCCCCAATTGGTCTAGTAAACATGTACAATTCGGGAGGAGCTATCGAAGGACTTAGATATGAAGCCGAGAAGATGAAAGTGGTAATGGAAGTTAAAGGATGTGGCAAATTCGGATCTTACTCTTCTGTGAAGCCTAAGAGATGCGTTGTTGAGTCAAATGAGATTGCATTCGAGTACGATTCCTCCTCTGGATTGGTCACCTTTGAGTTAGACAAAATGCCTATAGAGAACAAACGATTTCATCTGATCCAAGTTGAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

749

Amino Acids

83.12

Weight (kDa)

6.22

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 731 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1395
AatII GACGTC 1 cut(s) 98
AccB7I CCANNNNNTGG 2 cut(s) 247, 857
AccI GTMKAC 1 cut(s) 1600
AccII CGCG 3 cut(s) 638, 926, 1611
AciI CCGC 5 cut(s) 17, 693, 707, 945, 1188
AclI AACGTT 2 cut(s) 420, 855
AcsI RAATTY 1 cut(s) 2093
AcyI GRCGYC 3 cut(s) 95, 342, 666
AfaI GTAC 8 cut(s) 75, 174, 184, 511, 703, 1679, 2012, 2162
AfiI CCNNNNNNNGG 6 cut(s) 122, 247, 857, 1259, 1288, 1482
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 3 cut(s) 636, 915, 2007
AgeI ACCGGT 2 cut(s) 68, 115
AgsI TTSAA 2 cut(s) 904, 1806
AjiI CACGTC 2 cut(s) 460, 1026
AjnI CCWGG 4 cut(s) 598, 1522, 1594, 1835
Alw21I GWGCWC 5 cut(s) 441, 629, 1455, 1588, 1935
Alw26I GTCTC 8 cut(s) 272, 413, 690, 1160, 1368, 1839, 1908, 1918
Alw44I GTGCAC 2 cut(s) 625, 1451
Ama87I CYCGRG 1 cut(s) 956
AoxI GGCC 6 cut(s) 237, 259, 768, 1485, 1499, 1815
ApaLI GTGCAC 2 cut(s) 625, 1451
ApeKI GCWGC 7 cut(s) 1048, 1263, 1316, 1423, 1460, 1715, 1725
ApoI RAATTY 1 cut(s) 2093
AsiGI ACCGGT 2 cut(s) 68, 115
Asp700I GAANNNNTTC 1 cut(s) 569
AspS9I GGNCC 6 cut(s) 113, 238, 259, 312, 1485, 1499
AsuC2I CCSGG 4 cut(s) 199, 957, 958, 1253
AsuHPI GGTGA 4 cut(s) 467, 783, 1960, 2176
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 2 cut(s) 113, 312
BaeGI GKGCMC 2 cut(s) 629, 1455
BaeI ACNNNNGTAYC 2 cut(s) 57, 90
BamHI GGATCC 1 cut(s) 1354
BanII GRGCYC 2 cut(s) 441, 1955
BauI CACGAG 1 cut(s) 1934
BbsI GAAGAC 2 cut(s) 121, 1037
Bbv12I GWGCWC 5 cut(s) 441, 629, 1455, 1588, 1935
BbvI GCAGC 7 cut(s) 1035, 1250, 1328, 1435, 1447, 1727, 1737
BccI CCATC 8 cut(s) 229, 302, 615, 1379, 1496, 1562, 1634, 1967
BceAI ACGGC 2 cut(s) 355, 364
BciT130I CCWGG 4 cut(s) 600, 1524, 1596, 1837
BcnI CCSGG 4 cut(s) 199, 957, 958, 1253
BcoDI GTCTC 8 cut(s) 272, 413, 690, 1160, 1368, 1839, 1908, 1918
BfaI CTAG 2 cut(s) 1482, 2000
BfmI CTRYAG 2 cut(s) 1461, 2208
BfrI CTTAAG 1 cut(s) 680
BglI GCCNNNNNGGC 1 cut(s) 1776
BglII AGATCT 1 cut(s) 1938
BisI GCNGC 9 cut(s) 693, 707, 1049, 1264, 1317, 1424, 1461, 1716, 1726
BlsI GCNGC 9 cut(s) 694, 708, 1050, 1265, 1318, 1425, 1462, 1717, 1727
Bme1390I CCNGG 8 cut(s) 199, 600, 957, 958, 1253, 1524, 1596, 1837
Bme18I GGWCC 2 cut(s) 113, 312
BmeT110I CYCGRG 1 cut(s) 956
BmgBI CACGTC 2 cut(s) 460, 1026
BmgT120I GGNCC 6 cut(s) 113, 238, 259, 312, 1485, 1499
BmiI GGNNCC 4 cut(s) 313, 314, 1356, 1780
BmrFI CCNGG 8 cut(s) 199, 600, 957, 958, 1253, 1524, 1596, 1837
BmrI ACTGGG 1 cut(s) 1441
BmsI GCATC 7 cut(s) 110, 568, 745, 1111, 1282, 1340, 2117
BmuI ACTGGG 1 cut(s) 1441
BpiI GAAGAC 2 cut(s) 121, 1037
BpmI CTGGAG 1 cut(s) 1704
Bpu10I CCTNAGC 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 4 cut(s) 199, 957, 958, 1253
Bsa29I ATCGAT 1 cut(s) 1574
BsaAI YACGTR 1 cut(s) 916
BsaBI GATNNNNATC 1 cut(s) 1344
BsaHI GRCGYC 3 cut(s) 95, 342, 666
BsaJI CCNNGG 8 cut(s) 412, 599, 759, 956, 957, 1349, 1594, 1708
BsaWI WCCGGW 5 cut(s) 54, 68, 115, 216, 1357
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 2153, 2183
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 247, 857, 1259, 1288, 1482
Bse118I RCCGGY 5 cut(s) 13, 68, 115, 506, 695
Bse1I ACTGG 4 cut(s) 1436, 1687, 1780, 1910
Bse3DI GCAATG 1 cut(s) 1266
Bse8I GATNNNNATC 1 cut(s) 1344
BseBI CCWGG 4 cut(s) 600, 1524, 1596, 1837
BseCI ATCGAT 1 cut(s) 1574
BseDI CCNNGG 8 cut(s) 412, 599, 759, 956, 957, 1349, 1594, 1708
BseGI GGATG 7 cut(s) 240, 626, 850, 1153, 1297, 1371, 2090
BseJI GATNNNNATC 1 cut(s) 1344
BseLI CCNNNNNNNGG 6 cut(s) 122, 247, 857, 1259, 1288, 1482
BseMI GCAATG 1 cut(s) 1266
BseMII CTCAG 1 cut(s) 671
BseNI ACTGG 4 cut(s) 1436, 1687, 1780, 1910
BseRI GAGGAG 3 cut(s) 1222, 2037, 2161
BseSI GKGCMC 2 cut(s) 629, 1455
BseXI GCAGC 7 cut(s) 1035, 1250, 1328, 1435, 1447, 1727, 1737
BsgI GTGCAG 1 cut(s) 1172
Bsh1236I CGCG 3 cut(s) 638, 926, 1611
Bsh1285I CGRYCG 3 cut(s) 180, 476, 1192
BshFI GGCC 6 cut(s) 239, 261, 770, 1487, 1501, 1817
BshTI ACCGGT 2 cut(s) 68, 115
BshVI ATCGAT 1 cut(s) 1574
BsiEI CGRYCG 3 cut(s) 180, 476, 1192
BsiHKAI GWGCWC 5 cut(s) 441, 629, 1455, 1588, 1935
BsiHKCI CYCGRG 1 cut(s) 956
BslFI GGGAC 2 cut(s) 298, 647
BslI CCNNNNNNNGG 6 cut(s) 122, 247, 857, 1259, 1288, 1482
BsmAI GTCTC 8 cut(s) 272, 413, 690, 1160, 1368, 1839, 1908, 1918
BsmBI CGTCTC 1 cut(s) 272
BsmFI GGGAC 2 cut(s) 298, 647
BsmI GAATGC 2 cut(s) 1801, 2153
BsnI GGCC 6 cut(s) 239, 261, 770, 1487, 1501, 1817
BsoBI CYCGRG 1 cut(s) 956
Bsp1286I GDGCHC 6 cut(s) 441, 629, 1455, 1588, 1935, 1955
Bsp1407I TGTACA 1 cut(s) 2010
Bsp68I TCGCGA 1 cut(s) 1611
BspACI CCGC 5 cut(s) 17, 693, 707, 945, 1188
BspANI GGCC 6 cut(s) 239, 261, 770, 1487, 1501, 1817
BspCNI CTCAG 1 cut(s) 670
BspDI ATCGAT 1 cut(s) 1574
BspFNI CGCG 3 cut(s) 638, 926, 1611
BspLI GGNNCC 4 cut(s) 313, 314, 1356, 1780
BspMAI CTGCAG 1 cut(s) 1465
BspTI CTTAAG 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 1266
BsrFI RCCGGY 5 cut(s) 13, 68, 115, 506, 695
BsrGI TGTACA 1 cut(s) 2010
BsrI ACTGG 4 cut(s) 1436, 1687, 1780, 1910
BssAI RCCGGY 5 cut(s) 13, 68, 115, 506, 695
BssECI CCNNGG 8 cut(s) 412, 599, 759, 956, 957, 1349, 1594, 1708
BssNI GRCGYC 3 cut(s) 95, 342, 666
BssSI CACGAG 1 cut(s) 1934
BssT1I CCWWGG 2 cut(s) 412, 1708
Bst2BI CACGAG 1 cut(s) 1934
Bst2UI CCWGG 4 cut(s) 600, 1524, 1596, 1837
Bst6I CTCTTC 3 cut(s) 150, 1911, 2113
BstACI GRCGYC 3 cut(s) 95, 342, 666
BstAFI CTTAAG 1 cut(s) 680
BstAUI TGTACA 1 cut(s) 2010
BstBAI YACGTR 1 cut(s) 916
BstC8I GCNNGC 4 cut(s) 924, 1126, 1314, 1652
BstDEI CTNAG 6 cut(s) 657, 885, 1242, 1547, 2039, 2121
BstDSI CCRYGG 2 cut(s) 759, 1349
BstEII GGTNACC 1 cut(s) 2182
BstENI CCTNNNNNAGG 1 cut(s) 1480
BstF5I GGATG 7 cut(s) 240, 626, 850, 1153, 1297, 1371, 2090
BstFNI CGCG 3 cut(s) 638, 926, 1611
BstMAI GTCTC 8 cut(s) 272, 413, 690, 1160, 1368, 1839, 1908, 1918
BstMCI CGRYCG 3 cut(s) 180, 476, 1192
BstMWI GCNNNNNNNGC 5 cut(s) 107, 1388, 1561, 1631, 1776
BstNI CCWGG 4 cut(s) 600, 1524, 1596, 1837
BstNSI RCATGY 1 cut(s) 2011
BstPI GGTNACC 1 cut(s) 2182
BstSCI CCNGG 8 cut(s) 197, 598, 955, 956, 1251, 1522, 1594, 1835
BstSFI CTRYAG 2 cut(s) 1461, 2208
BstSLI GKGCMC 2 cut(s) 629, 1455
BstUI CGCG 3 cut(s) 638, 926, 1611
BstV1I GCAGC 7 cut(s) 1035, 1250, 1328, 1435, 1447, 1727, 1737
BstV2I GAAGAC 2 cut(s) 121, 1037
BstX2I RGATCY 6 cut(s) 392, 514, 971, 1354, 1938, 2099
BstXI CCANNNNNNTGG 1 cut(s) 1836
BstYI RGATCY 6 cut(s) 392, 514, 971, 1354, 1938, 2099
Bsu15I ATCGAT 1 cut(s) 1574
BsuRI GGCC 6 cut(s) 239, 261, 770, 1487, 1501, 1817
BsuTUI ATCGAT 1 cut(s) 1574
BtgI CCRYGG 2 cut(s) 759, 1349
BtrI CACGTC 2 cut(s) 460, 1026
BtsCI GGATG 7 cut(s) 240, 626, 850, 1153, 1297, 1371, 2090
BtsI GCAGTG 1 cut(s) 1160
BtsIMutI CAGTG 7 cut(s) 1160, 1405, 1498, 1519, 1680, 1773, 1821
BtuMI TCGCGA 1 cut(s) 1611
Cac8I GCNNGC 4 cut(s) 924, 1126, 1314, 1652
Cfr10I RCCGGY 5 cut(s) 13, 68, 115, 506, 695
Cfr13I GGNCC 6 cut(s) 113, 238, 259, 312, 1485, 1499
Cfr9I CCCGGG 1 cut(s) 956
ClaI ATCGAT 1 cut(s) 1574
CseI GACGC 6 cut(s) 289, 331, 545, 644, 655, 1054
Csp6I GTAC 8 cut(s) 74, 173, 183, 510, 702, 1678, 2011, 2161
CspAI ACCGGT 2 cut(s) 68, 115
CspCI CAANNNNNGTGG 2 cut(s) 987, 1022
CviAII CATG 3 cut(s) 1472, 1669, 2008
CviQI GTAC 8 cut(s) 74, 173, 183, 510, 702, 1678, 2011, 2161
DdeI CTNAG 6 cut(s) 657, 885, 1242, 1547, 2039, 2121
Eam1104I CTCTTC 3 cut(s) 150, 1911, 2113
EarI CTCTTC 3 cut(s) 150, 1911, 2113
EciI GGCGGA 1 cut(s) 960
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 412, 1708
Eco147I AGGCCT 1 cut(s) 1817
Eco24I GRGCYC 2 cut(s) 441, 1955
Eco47I GGWCC 2 cut(s) 113, 312
Eco53kI GAGCTC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 956
Eco91I GGTNACC 1 cut(s) 2182
EcoICRI GAGCTC 1 cut(s) 439
EcoNI CCTNNNNNAGG 1 cut(s) 1480
EcoO109I RGGNCCY 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 2182
EcoRII CCWGG 4 cut(s) 598, 1522, 1594, 1835
EcoT14I CCWWGG 2 cut(s) 412, 1708
EcoT38I GRGCYC 2 cut(s) 441, 1955
ErhI CCWWGG 2 cut(s) 412, 1708
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 3 cut(s) 1475, 1672, 2011
FalI AAGNNNNNCTT 4 cut(s) 663, 695, 1520, 1552
FaqI GGGAC 2 cut(s) 298, 647
FatI CATG 3 cut(s) 1471, 1668, 2007
FblI GTMKAC 1 cut(s) 1600
Fnu4HI GCNGC 9 cut(s) 693, 707, 1049, 1264, 1317, 1424, 1461, 1716, 1726
FokI GGATG 7 cut(s) 247, 633, 857, 1160, 1304, 1358, 2097
FriOI GRGCYC 2 cut(s) 441, 1955
Fsp4HI GCNGC 9 cut(s) 693, 707, 1049, 1264, 1317, 1424, 1461, 1716, 1726
FspBI CTAG 2 cut(s) 1482, 2000
GluI GCNGC 9 cut(s) 693, 707, 1049, 1264, 1317, 1424, 1461, 1716, 1726
GsuI CTGGAG 1 cut(s) 1704
HaeIII GGCC 6 cut(s) 239, 261, 770, 1487, 1501, 1817
HgaI GACGC 6 cut(s) 289, 331, 545, 644, 655, 1054
Hin1I GRCGYC 3 cut(s) 95, 342, 666
Hin1II CATG 3 cut(s) 1475, 1672, 2011
HincII GTYRAC 3 cut(s) 424, 578, 610
HindII GTYRAC 3 cut(s) 424, 578, 610
HindIII AAGCTT 2 cut(s) 593, 1553
HpaI GTTAAC 1 cut(s) 424
HphI GGTGA 4 cut(s) 467, 783, 1960, 2176
Hpy99I CGWCG 8 cut(s) 35, 97, 137, 347, 481, 671, 734, 1027
HpyAV CCTTC 5 cut(s) 116, 383, 656, 835, 2027
HpyCH4IV ACGT 7 cut(s) 95, 420, 459, 580, 855, 915, 1025
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 1388, 1561, 1631, 1776
HpyF3I CTNAG 6 cut(s) 657, 885, 1242, 1547, 2039, 2121
HpySE526I ACGT 7 cut(s) 95, 420, 459, 580, 855, 915, 1025
Hsp92I GRCGYC 3 cut(s) 95, 342, 666
Hsp92II CATG 3 cut(s) 1475, 1672, 2011
KflI GGGWCCC 1 cut(s) 312
KspAI GTTAAC 1 cut(s) 424
LmnI GCTCC 6 cut(s) 137, 332, 1712, 1722, 1784, 2024
Lsp1109I GCAGC 7 cut(s) 1035, 1250, 1328, 1435, 1447, 1727, 1737
LweI GCATC 7 cut(s) 110, 568, 745, 1111, 1282, 1340, 2117
MaeI CTAG 2 cut(s) 1482, 2000
MaeII ACGT 7 cut(s) 95, 420, 459, 580, 855, 915, 1025
MaeIII GTNAC 7 cut(s) 350, 455, 581, 652, 789, 1109, 2182
MfeI CAATTG 1 cut(s) 1992
MflI RGATCY 6 cut(s) 392, 514, 971, 1354, 1938, 2099
MhlI GDGCHC 6 cut(s) 441, 629, 1455, 1588, 1935, 1955
MluCI AATT 4 cut(s) 193, 1992, 2014, 2093
MluI ACGCGT 1 cut(s) 636
MlyI GAGTC 8 cut(s) 135, 332, 649, 797, 1198, 1223, 1584, 2147
MmeI TCCRAC 4 cut(s) 54, 115, 1046, 1853
MroXI GAANNNNTTC 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 1319
MspCI CTTAAG 1 cut(s) 680
MspR9I CCNGG 8 cut(s) 199, 600, 957, 958, 1253, 1524, 1596, 1837
MunI CAATTG 1 cut(s) 1992
Mva1269I GAATGC 2 cut(s) 1801, 2153
MvaI CCWGG 4 cut(s) 600, 1524, 1596, 1837
MvnI CGCG 3 cut(s) 638, 926, 1611
MwoI GCNNNNNNNGC 5 cut(s) 107, 1388, 1561, 1631, 1776
NciI CCSGG 4 cut(s) 199, 957, 958, 1253
NlaIII CATG 3 cut(s) 1475, 1672, 2011
NlaIV GGNNCC 4 cut(s) 313, 314, 1356, 1780
NmeAIII GCCGAG 2 cut(s) 287, 2077
NmuCI GTSAC 5 cut(s) 455, 581, 652, 789, 2182
NruI TCGCGA 1 cut(s) 1611
NspI RCATGY 1 cut(s) 2011
PceI AGGCCT 1 cut(s) 1817
PciI ACATGT 1 cut(s) 2007
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 2 cut(s) 1801, 2153
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 5 cut(s) 37, 61, 1517, 1576, 2165
PflMI CCANNNNNTGG 2 cut(s) 247, 857
PfoI TCCNGGA 2 cut(s) 1251, 1522
PinAI ACCGGT 2 cut(s) 68, 115
PkrI GCNGC 9 cut(s) 694, 708, 1050, 1265, 1318, 1425, 1462, 1717, 1727
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 8 cut(s) 134, 331, 649, 796, 1198, 1223, 1584, 2146
PpsI GAGTC 8 cut(s) 134, 331, 649, 796, 1198, 1223, 1584, 2146
Ppu21I YACGTR 1 cut(s) 916
PpuMI RGGWCCY 1 cut(s) 312
PscI ACATGT 1 cut(s) 2007
PsiI TTATAA 1 cut(s) 1395
Psp124BI GAGCTC 1 cut(s) 441
Psp1406I AACGTT 2 cut(s) 420, 855
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 4 cut(s) 598, 1522, 1594, 1835
PspEI GGTNACC 1 cut(s) 2182
PspGI CCWGG 4 cut(s) 598, 1522, 1594, 1835
PspN4I GGNNCC 4 cut(s) 313, 314, 1356, 1780
PspPI GGNCC 6 cut(s) 113, 238, 259, 312, 1485, 1499
PspPPI RGGWCCY 1 cut(s) 312
PstI CTGCAG 1 cut(s) 1465
PsuI RGATCY 6 cut(s) 392, 514, 971, 1354, 1938, 2099
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 1319
RruI TCGCGA 1 cut(s) 1611
RsaI GTAC 8 cut(s) 75, 174, 184, 511, 703, 1679, 2012, 2162
RsaNI GTAC 8 cut(s) 74, 173, 183, 510, 702, 1678, 2011, 2161
SacI GAGCTC 1 cut(s) 441
SatI GCNGC 9 cut(s) 693, 707, 1049, 1264, 1317, 1424, 1461, 1716, 1726
Sau96I GGNCC 6 cut(s) 113, 238, 259, 312, 1485, 1499
SchI GAGTC 8 cut(s) 135, 332, 649, 797, 1198, 1223, 1584, 2147
ScrFI CCNGG 8 cut(s) 199, 600, 957, 958, 1253, 1524, 1596, 1837
SduI GDGCHC 6 cut(s) 441, 629, 1455, 1588, 1935, 1955
SfaNI GCATC 7 cut(s) 110, 568, 745, 1111, 1282, 1340, 2117
SfcI CTRYAG 2 cut(s) 1461, 2208
SgrAI CRCCGGYG 1 cut(s) 695
SinI GGWCC 2 cut(s) 113, 312
SmaI CCCGGG 1 cut(s) 958
SmlI CTYRAG 2 cut(s) 320, 680
SmoI CTYRAG 2 cut(s) 320, 680
Sse9I AATT 4 cut(s) 193, 1992, 2014, 2093
SseBI AGGCCT 1 cut(s) 1817
SsiI CCGC 5 cut(s) 17, 693, 707, 945, 1188
SspMI CTAG 2 cut(s) 1482, 2000
SstI GAGCTC 1 cut(s) 441
StuI AGGCCT 1 cut(s) 1817
StyD4I CCNGG 8 cut(s) 197, 598, 955, 956, 1251, 1522, 1594, 1835
StyI CCWWGG 2 cut(s) 412, 1708
TaiI ACGT 7 cut(s) 98, 423, 462, 583, 858, 918, 1028
TasI AATT 4 cut(s) 193, 1992, 2014, 2093
TatI WGTACW 2 cut(s) 1677, 2010
TauI GCSGC 2 cut(s) 695, 709
TfiI GAWTC 5 cut(s) 37, 61, 1517, 1576, 2165
TscAI CASTG 7 cut(s) 1160, 1405, 1498, 1519, 1687, 1780, 1828
TseFI GTSAC 5 cut(s) 455, 581, 652, 789, 2182
TseI GCWGC 7 cut(s) 1048, 1263, 1316, 1423, 1460, 1715, 1725
Tsp45I GTSAC 5 cut(s) 455, 581, 652, 789, 2182
TspDTI ATGAA 7 cut(s) 995, 1094, 1205, 1685, 2061, 2075, 2216
TspMI CCCGGG 1 cut(s) 956
TspRI CASTG 7 cut(s) 1160, 1405, 1498, 1519, 1687, 1780, 1828
Van91I CCANNNNNTGG 2 cut(s) 247, 857
Vha464I CTTAAG 1 cut(s) 680
VneI GTGCAC 2 cut(s) 625, 1451
VpaK11BI GGWCC 2 cut(s) 113, 312
XagI CCTNNNNNAGG 1 cut(s) 1480
XapI RAATTY 1 cut(s) 2093
XceI RCATGY 1 cut(s) 2011
XmaI CCCGGG 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 1600
XmnI GAANNNNTTC 1 cut(s) 569
XspI CTAG 2 cut(s) 1482, 2000
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.