Rh2AG031200

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009084
Physical Location & Seq
Forward (+)
2167672 .. 2171071
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG031200.1

Sequence Viewer

Length: 2190 bp
ATGACGATTAAGCCGGCGGTTCGCGTTTCCGACGGGAAACTGATAGTGAAGAACCGGACCGTTCTGACCGGATTACCGGACAATGTGGTCGCCACGTCCGGTTCGGCCTCCGGTCCCGCCGAGGGAGTCTTCCTCGGCTCGGCGTTCGAGGAGGGCAGCAGCCGGCACGTGACTTCTCTCGGAACTTTTCAAAACGGCGTGCGTTTCATGGCGTGCTTCCGGTTCAAGCTATGGTGGATGGCCCAGAAAATGGGCCGCTCCGGCCGCGACGTCCCTCTCGAGACTCAATTCCTATTGGTCGAGACCCAAGACGGGTCCCACCTCGAAGAGGACCAGGTCGTGTACGCCGTGTTCCTCCCTCTTATCGAAGGCCCCTTCAGGGCTAGCTTACAGGGGAACAGCGGAGACGAGCTGGAGCTTTGCTTGGAGAGCGGCGACGTGGACACCAAAGCGGCGTCGTTTAGCCACGCGGTGTTCGTTCACGCCGGGACGGACCCGTTCGCGACGATAACGGAGGCGGTCAGGGCCGTTAAGGTCCACCTGAAGACGTTTCGTCAACGTCACGAGAAGAAGCTCCCTGGAATCGTCGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCAGGACGTGACTCAGGAAGGAGTTGAGGCCGGCCTCAAGTCCCTCACCGCCGGCGGAGCGCCGCCGAAGTTTGTCATCATCGACGACGGGTGGCAGTCCGTCGCCGGCGATCAGGACAAAGCTAGCGAGCTACAGAGACTGACCGGAATCAAAGAAAACTCGAAGTTTCAACACAAGGATGATGGGATCATGACGATAGTGAACATTGCAAAAGAAAAATACGGGTTGAAGTACGTGTACGTCTGGCACGCGATTACCGGCTACTGGGGAGGAGTCCGGCCCGGAACTGAATACGGGTCGGTTCTGAAGTACCCGGATTTGTCGAAAGGGGTTATGGAAAACGAACCGGCCTGGAAAACGGACATGATCCGGTTACAGGGTCTGGGCTTAGTGAACCCGAAAAGCGTGTACAAGTTTTACAACGACCTACACAGCTACTTGGCCTCTGCGGGTGTAGATGGGGTTAAGGTGGACGCGCAGTGCGTGCTGGAGACTCTCGGAGCCGGGCTGGGAGGGCGGGTCGAGCTGACCCGGCAGTACCACCAGGCATTGGATGCTTCGGTGTCTCGGAATTTTGAAGATAATGGGATCATAGCATGCATGAGCCATAATACTGATGCGCTGTACTGTGAGAAACAGACGGCGGTGGTGAGAGCCTCCGACGATTTCTACCCGCGCGATCCGATGTCGCATACGATCCACGTGGCGGCGGTGGCGTATAATAGCGTTTTCCTCGGCGAGTTTATGCTGCCGGATTGGGATATGTTCCACTCGCTCCACCCCGCGGCTGAGTACCACGCGTCCGCTAGAGCCCTTAGTGGTGGACCTGTTTATGTTAGTGATGCGCCTGGGAAGCATGACTTTGAGCTTTTGAAGAAGATGGTATTGCCGGACGGGTCGGTGCTCCGGGCGCGGTTACCCGGAAGGCCGACCCGGGACTGCTTGTTCACTGACCCGGCCCGGGATGGGGTGAGTTTGTTGAAGATATGGAACATGAATAAGTACACAGGTGTAGTGGGGGTGTACAATTGCCAAGGAGCAGCATGGAGCTCACTGGAGAGAAAGAATGCATTCCACGAGACGAAATCGGACGCCATTACCGGTTATGTTCGCGGCCGTGACGTCCACCTCATTAAGGAGGCAGCCACTGATCCCGATTGGAATGGTGACTGCGCCGTGTATTGCAATCGGACAGGGGAGCTTGTGACATTGCCTCACAATGCTGCCATGCCTATTTCCCTCAAAGTGCTTGAACATGAGATTTTCACTGTCAGCCCCATCAGGGTTTTGGCGCCTGGCGTGATATTCGCTCCTTTGGGACTGGTTGACATGTACAATGCTGGTGGAGCCATTGAAGACTTGAGATATGAGCAGAGATTAGTCTCTATGGAGGTTAGAGGGTGTGGCAAGTTTGGTGTGTATTCTTCAGAGAAGCCCATGAGGTGCTGTGTAGGGACTAATGGGGTTGATTTCAGCTATGATTCGGCATCTGGGTTGGTGACTTTGATCTTGGATCATATGCCTGAAGAGGGAAAAAGAGTACAACTAGTCGAGGTTGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

729

Amino Acids

80.1

Weight (kDa)

5.79

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 710 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 273, 1749
AccB1I GGYRCC 1 cut(s) 1915
AccB7I CCANNNNNTGG 2 cut(s) 250, 1174
AccBSI CCGCTC 2 cut(s) 258, 432
AccI GTMKAC 1 cut(s) 588
AclWI GGATC 7 cut(s) 818, 985, 1220, 1298, 1315, 1769, 2145
AcoI YGGCCR 2 cut(s) 262, 1738
AcsI RAATTY 1 cut(s) 1195
AcuI CTGAAG 5 cut(s) 361, 563, 950, 2034, 2169
AcvI CACGTG 2 cut(s) 169, 1327
AcyI GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
AdeI CACNNNGTG 1 cut(s) 472
AflIII ACRYGT 4 cut(s) 615, 858, 1422, 1953
AgeI ACCGGT 1 cut(s) 1724
AgsI TTSAA 9 cut(s) 191, 226, 794, 853, 1202, 1498, 1606, 1877, 1979
AhdI GACNNNNNGTC 1 cut(s) 552
AhlI ACTAGT 1 cut(s) 2170
AjiI CACGTC 3 cut(s) 96, 439, 631
AjnI CCWGG 8 cut(s) 333, 577, 608, 624, 974, 1167, 1471, 1918
AloI GAACNNNNNNTCC 2 cut(s) 1553, 1585
Alw21I GWGCWC 3 cut(s) 608, 1530, 1676
Alw26I GTCTC 8 cut(s) 275, 296, 399, 754, 1109, 1194, 1697, 2011
Alw44I GTGCAC 1 cut(s) 604
AlwI GGATC 7 cut(s) 818, 985, 1220, 1298, 1315, 1769, 2145
AlwNI CAGNNNCTG 3 cut(s) 763, 1006, 1772
Ama87I CYCGRG 3 cut(s) 278, 1557, 1584
ApaLI GTGCAC 1 cut(s) 604
ApeKI GCWGC 6 cut(s) 156, 159, 1372, 1664, 1766, 1847
ApoI RAATTY 1 cut(s) 1195
AsiGI ACCGGT 1 cut(s) 1724
Asp700I GAANNNNTTC 1 cut(s) 1694
AspLEI GCGC 8 cut(s) 685, 1102, 1246, 1302, 1471, 1537, 1799, 1918
AsuHPI GGTGA 5 cut(s) 661, 1285, 1606, 1802, 2134
AsuNHI GCTAGC 2 cut(s) 383, 746
AvaI CYCGRG 3 cut(s) 278, 1557, 1584
AvaII GGWCC 7 cut(s) 57, 113, 315, 331, 493, 535, 1448
BaeGI GKGCMC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 1915
BanII GRGCYC 2 cut(s) 1438, 1676
BarI GAAGNNNNNNTAC 2 cut(s) 845, 877
BauI CACGAG 2 cut(s) 563, 1700
BbrPI CACGTG 2 cut(s) 169, 1327
BbsI GAAGAC 3 cut(s) 121, 551, 1986
Bbv12I GWGCWC 3 cut(s) 608, 1530, 1676
BbvI GCAGC 6 cut(s) 168, 171, 1359, 1676, 1778, 1834
BccI CCATC 6 cut(s) 232, 800, 1076, 1498, 1583, 1910
BceAI ACGGC 6 cut(s) 211, 332, 512, 1281, 1725, 1784
BciT130I CCWGG 8 cut(s) 335, 579, 610, 626, 976, 1169, 1473, 1920
BcoDI GTCTC 8 cut(s) 275, 296, 399, 754, 1109, 1194, 1697, 2011
BcuI ACTAGT 1 cut(s) 2170
BfaI CTAG 4 cut(s) 384, 747, 1431, 2171
BfmI CTRYAG 1 cut(s) 755
BfoI RGCGCY 2 cut(s) 686, 1919
BglI GCCNNNNNGGC 1 cut(s) 261
Bme18I GGWCC 7 cut(s) 57, 113, 315, 331, 493, 535, 1448
BmeRI GACNNNNNGTC 1 cut(s) 552
BmeT110I CYCGRG 3 cut(s) 278, 1557, 1584
BmgBI CACGTC 3 cut(s) 96, 439, 631
BmiI GGNNCC 8 cut(s) 115, 316, 317, 373, 495, 1126, 1917, 1972
BmrI ACTGGG 1 cut(s) 898
BmsI GCATC 4 cut(s) 1168, 1231, 1456, 2120
BmtI GCTAGC 2 cut(s) 387, 750
BmuI ACTGGG 1 cut(s) 898
BpiI GAAGAC 3 cut(s) 121, 551, 1986
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpmI CTGGAG 3 cut(s) 434, 1133, 1700
BpuEI CTTGAG 2 cut(s) 644, 2005
BsaAI YACGTR 3 cut(s) 169, 859, 1327
BsaHI GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
BsaI GGTCTC 1 cut(s) 296
BsaWI WCCGGW 9 cut(s) 54, 68, 76, 98, 110, 219, 767, 993, 1724
BsaXI ACNNNNNCTCC 2 cut(s) 1128, 1158
Bse118I RCCGGY 8 cut(s) 13, 162, 653, 674, 728, 881, 970, 1724
Bse1I ACTGG 3 cut(s) 893, 1683, 1950
Bse3DI GCAATG 2 cut(s) 828, 1832
BseBI CCWGG 8 cut(s) 335, 579, 610, 626, 976, 1169, 1473, 1920
BseGI GGATG 4 cut(s) 243, 808, 1183, 1594
BseMI GCAATG 2 cut(s) 828, 1832
BseMII CTCAG 2 cut(s) 650, 1404
BseNI ACTGG 3 cut(s) 893, 1683, 1950
BseRI GAGGAG 2 cut(s) 164, 909
BseSI GKGCMC 1 cut(s) 608
BseX3I CGGCCG 2 cut(s) 262, 1738
BseXI GCAGC 6 cut(s) 168, 171, 1359, 1676, 1778, 1834
BseYI CCCAGC 1 cut(s) 1132
Bsh1285I CGRYCG 2 cut(s) 265, 1741
BshNI GGYRCC 1 cut(s) 1915
BshTI ACCGGT 1 cut(s) 1724
BsiEI CGRYCG 2 cut(s) 265, 1741
BsiHKAI GWGCWC 3 cut(s) 608, 1530, 1676
BsiHKCI CYCGRG 3 cut(s) 278, 1557, 1584
BslFI GGGAC 9 cut(s) 99, 257, 301, 502, 626, 649, 1574, 1956, 2092
BsmAI GTCTC 8 cut(s) 275, 296, 399, 754, 1109, 1194, 1697, 2011
BsmBI CGTCTC 2 cut(s) 399, 1697
BsmFI GGGAC 9 cut(s) 99, 257, 301, 502, 626, 649, 1574, 1956, 2092
BsmI GAATGC 2 cut(s) 1694, 1696
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 3 cut(s) 278, 1557, 1584
Bsp1286I GDGCHC 4 cut(s) 608, 1438, 1530, 1676
Bsp1407I TGTACA 3 cut(s) 1032, 1647, 1956
Bsp143I GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
Bsp68I TCGCGA 1 cut(s) 503
BspCNI CTCAG 2 cut(s) 649, 1405
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 8 cut(s) 115, 316, 317, 373, 495, 1126, 1917, 1972
BspOI GCTAGC 2 cut(s) 387, 750
BspPI GGATC 7 cut(s) 818, 985, 1220, 1298, 1315, 1769, 2145
BspT107I GGYRCC 1 cut(s) 1915
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 2 cut(s) 258, 432
BsrDI GCAATG 2 cut(s) 828, 1832
BsrFI RCCGGY 8 cut(s) 13, 162, 653, 674, 728, 881, 970, 1724
BsrGI TGTACA 3 cut(s) 1032, 1647, 1956
BsrI ACTGG 3 cut(s) 893, 1683, 1950
BssAI RCCGGY 8 cut(s) 13, 162, 653, 674, 728, 881, 970, 1724
BssMI GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
BssNI GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
BssSI CACGAG 2 cut(s) 563, 1700
BssT1I CCWWGG 1 cut(s) 1657
Bst2BI CACGAG 2 cut(s) 563, 1700
Bst2UI CCWGG 8 cut(s) 335, 579, 610, 626, 976, 1169, 1473, 1920
Bst4CI ACNGT 3 cut(s) 61, 1253, 1894
Bst6I CTCTTC 2 cut(s) 321, 2145
BstACI GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
BstAPI GCANNNNNTGC 2 cut(s) 1108, 1178
BstAUI TGTACA 3 cut(s) 1032, 1647, 1956
BstBAI YACGTR 3 cut(s) 169, 859, 1327
BstDEI CTNAG 4 cut(s) 636, 1012, 1413, 1439
BstDSI CCRYGG 1 cut(s) 1407
BstEII GGTNACC 1 cut(s) 1539
BstENI CCTNNNNNAGG 2 cut(s) 326, 1757
BstF5I GGATG 4 cut(s) 243, 808, 1183, 1594
BstH2I RGCGCY 2 cut(s) 686, 1919
BstHHI GCGC 8 cut(s) 685, 1102, 1246, 1302, 1471, 1537, 1799, 1918
BstKTI GATC 9 cut(s) 736, 813, 993, 1215, 1306, 1323, 1777, 2133, 2140
BstMAI GTCTC 8 cut(s) 275, 296, 399, 754, 1109, 1194, 1697, 2011
BstMBI GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
BstMCI CGRYCG 2 cut(s) 265, 1741
BstNI CCWGG 8 cut(s) 335, 579, 610, 626, 976, 1169, 1473, 1920
BstNSI RCATGY 2 cut(s) 1224, 1957
BstPI GGTNACC 1 cut(s) 1539
BstSFI CTRYAG 1 cut(s) 755
BstSLI GKGCMC 1 cut(s) 608
BstV1I GCAGC 6 cut(s) 168, 171, 1359, 1676, 1778, 1834
BstV2I GAAGAC 3 cut(s) 121, 551, 1986
BstZI CGGCCG 2 cut(s) 262, 1738
BtgI CCRYGG 1 cut(s) 1407
BtrI CACGTC 3 cut(s) 96, 439, 631
BtsCI GGATG 4 cut(s) 243, 808, 1183, 1594
BtsI GCAGTG 1 cut(s) 1109
BtsIMutI CAGTG 5 cut(s) 1109, 1572, 1676, 1770, 1890
BtuMI TCGCGA 1 cut(s) 503
CaiI CAGNNNCTG 3 cut(s) 763, 1006, 1772
CciI TCATGA 1 cut(s) 813
CfoI GCGC 8 cut(s) 685, 1102, 1246, 1302, 1471, 1537, 1799, 1918
Cfr10I RCCGGY 8 cut(s) 13, 162, 653, 674, 728, 881, 970, 1724
Cfr42I CCGCGG 1 cut(s) 1410
Cfr9I CCCGGG 2 cut(s) 1557, 1584
CpoI CGGWCCG 1 cut(s) 57
CseI GACGC 5 cut(s) 444, 623, 1106, 1413, 1724
CsiI ACCWGGT 1 cut(s) 333
CspAI ACCGGT 1 cut(s) 1724
CspCI CAANNNNNGTGG 2 cut(s) 1948, 1983
CspI CGGWCCG 1 cut(s) 57
DdeI CTNAG 4 cut(s) 636, 1012, 1413, 1439
DinI GGCGCC 1 cut(s) 1917
DpnI GATC 9 cut(s) 735, 812, 992, 1214, 1305, 1322, 1776, 2132, 2139
DpnII GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
DraIII CACNNNGTG 1 cut(s) 472
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 552
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 262, 1738
EagI CGGCCG 2 cut(s) 262, 1738
Eam1104I CTCTTC 2 cut(s) 321, 2145
Eam1105I GACNNNNNGTC 1 cut(s) 552
EarI CTCTTC 2 cut(s) 321, 2145
EciI GGCGGA 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 1674
EclXI CGGCCG 2 cut(s) 262, 1738
Eco130I CCWWGG 1 cut(s) 1657
Eco24I GRGCYC 2 cut(s) 1438, 1676
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 7 cut(s) 57, 113, 315, 331, 493, 535, 1448
Eco52I CGGCCG 2 cut(s) 262, 1738
Eco53kI GAGCTC 1 cut(s) 1674
Eco57I CTGAAG 5 cut(s) 361, 563, 950, 2034, 2169
Eco72I CACGTG 2 cut(s) 169, 1327
Eco88I CYCGRG 3 cut(s) 278, 1557, 1584
Eco91I GGTNACC 1 cut(s) 1539
EcoICRI GAGCTC 1 cut(s) 1674
EcoNI CCTNNNNNAGG 2 cut(s) 326, 1757
EcoO109I RGGNCCY 2 cut(s) 315, 371
EcoO65I GGTNACC 1 cut(s) 1539
EcoRII CCWGG 8 cut(s) 333, 577, 608, 624, 974, 1167, 1471, 1918
EcoT14I CCWWGG 1 cut(s) 1657
EcoT22I ATGCAT 2 cut(s) 1226, 1696
EcoT38I GRGCYC 2 cut(s) 1438, 1676
EgeI GGCGCC 1 cut(s) 1917
EheI GGCGCC 1 cut(s) 1917
ErhI CCWWGG 1 cut(s) 1657
Esp3I CGTCTC 2 cut(s) 399, 1697
FalI AAGNNNNNCTT 2 cut(s) 1469, 1501
FaqI GGGAC 9 cut(s) 99, 257, 301, 502, 626, 649, 1574, 1956, 2092
FauI CCCGC 5 cut(s) 124, 1066, 1134, 1305, 1414
FauNDI CATATG 1 cut(s) 2142
FblI GTMKAC 1 cut(s) 588
FokI GGATG 4 cut(s) 250, 815, 1190, 1601
FriOI GRGCYC 2 cut(s) 1438, 1676
FseI GGCCGGCC 1 cut(s) 657
FspBI CTAG 4 cut(s) 384, 747, 1431, 2171
GlaI GCGC 8 cut(s) 684, 1101, 1245, 1301, 1470, 1536, 1798, 1917
GsaI CCCAGC 1 cut(s) 1136
GsuI CTGGAG 3 cut(s) 434, 1133, 1700
HaeII RGCGCY 2 cut(s) 686, 1919
HgaI GACGC 5 cut(s) 444, 623, 1106, 1413, 1724
HhaI GCGC 8 cut(s) 685, 1102, 1246, 1302, 1471, 1537, 1799, 1918
Hin1I GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
Hin6I GCGC 8 cut(s) 683, 1100, 1244, 1300, 1469, 1535, 1797, 1916
HinP1I GCGC 8 cut(s) 683, 1100, 1244, 1300, 1469, 1535, 1797, 1916
HincII GTYRAC 3 cut(s) 557, 589, 1951
HindII GTYRAC 3 cut(s) 557, 589, 1951
HinfI GANTC 8 cut(s) 126, 283, 582, 634, 771, 897, 1117, 2105
HphI GGTGA 5 cut(s) 661, 1285, 1606, 1802, 2134
Hpy188III TCNNGA 9 cut(s) 278, 280, 301, 502, 563, 638, 737, 814, 1778
HpyAV CCTTC 4 cut(s) 362, 385, 635, 1542
HpyCH4III ACNGT 3 cut(s) 61, 1253, 1894
HpyCH4V TGCA 5 cut(s) 606, 833, 1224, 1694, 1809
HpyF3I CTNAG 4 cut(s) 636, 1012, 1413, 1439
Hsp92I GRCGYC 5 cut(s) 270, 455, 1716, 1746, 1916
HspAI GCGC 8 cut(s) 683, 1100, 1244, 1300, 1469, 1535, 1797, 1916
KasI GGCGCC 1 cut(s) 1915
KflI GGGWCCC 1 cut(s) 315
KroI GCCGGC 5 cut(s) 13, 162, 653, 674, 728
KroNI GCCGGC 5 cut(s) 15, 164, 655, 676, 730
KspI CCGCGG 1 cut(s) 1410
Kzo9I GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
Lsp1109I GCAGC 6 cut(s) 168, 171, 1359, 1676, 1778, 1834
LweI GCATC 4 cut(s) 1168, 1231, 1456, 2120
MabI ACCWGGT 1 cut(s) 333
MaeI CTAG 4 cut(s) 384, 747, 1431, 2171
MaeIII GTNAC 9 cut(s) 169, 560, 631, 996, 1539, 1742, 1790, 1828, 2122
MalI GATC 9 cut(s) 735, 812, 992, 1214, 1305, 1322, 1776, 2132, 2139
MbiI CCGCTC 2 cut(s) 258, 432
MboI GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
MfeI CAATTG 1 cut(s) 1651
MhlI GDGCHC 4 cut(s) 608, 1438, 1530, 1676
MluCI AATT 3 cut(s) 287, 1195, 1651
MluI ACGCGT 2 cut(s) 615, 1422
Mly113I GGCGCC 1 cut(s) 1916
MlyI GAGTC 5 cut(s) 135, 277, 628, 906, 1111
MmeI TCCRAC 2 cut(s) 54, 1308
Mph1103I ATGCAT 2 cut(s) 1226, 1696
MreI CGCCGGCG 2 cut(s) 674, 728
MroNI GCCGGC 5 cut(s) 13, 162, 653, 674, 728
MroXI GAANNNNTTC 1 cut(s) 1694
MseI TTAA 4 cut(s) 9, 531, 1089, 1758
MslI CAYNNNNRTG 1 cut(s) 801
MspA1I CMGCKG 2 cut(s) 402, 1409
MunI CAATTG 1 cut(s) 1651
Mva1269I GAATGC 2 cut(s) 1694, 1696
MvaI CCWGG 8 cut(s) 335, 579, 610, 626, 976, 1169, 1473, 1920
NaeI GCCGGC 5 cut(s) 15, 164, 655, 676, 730
NarI GGCGCC 1 cut(s) 1916
NdeI CATATG 1 cut(s) 2142
NdeII GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
NgoMIV GCCGGC 5 cut(s) 13, 162, 653, 674, 728
NheI GCTAGC 2 cut(s) 383, 746
NlaIV GGNNCC 8 cut(s) 115, 316, 317, 373, 495, 1126, 1917, 1972
NmeAIII GCCGAG 4 cut(s) 114, 119, 145, 1338
NmuCI GTSAC 7 cut(s) 169, 560, 631, 1742, 1790, 1828, 2122
NruI TCGCGA 1 cut(s) 503
NsiI ATGCAT 2 cut(s) 1226, 1696
NspI RCATGY 2 cut(s) 1224, 1957
PaeI GCATGC 1 cut(s) 1224
PaeR7I CTCGAG 1 cut(s) 278
PagI TCATGA 1 cut(s) 813
PciI ACATGT 1 cut(s) 1953
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 345, 474, 1104
PctI GAATGC 2 cut(s) 1694, 1696
PdiI GCCGGC 5 cut(s) 15, 164, 655, 676, 730
PdmI GAANNNNTTC 1 cut(s) 1694
PfeI GAWTC 3 cut(s) 582, 771, 2105
PflFI GACNNNGTC 1 cut(s) 335
PflMI CCANNNNNTGG 2 cut(s) 250, 1174
PinAI ACCGGT 1 cut(s) 1724
PleI GAGTC 5 cut(s) 134, 277, 628, 905, 1111
PluTI GGCGCC 1 cut(s) 1919
PmaCI CACGTG 2 cut(s) 169, 1327
PmlI CACGTG 2 cut(s) 169, 1327
PpsI GAGTC 5 cut(s) 134, 277, 628, 905, 1111
Ppu21I YACGTR 3 cut(s) 169, 859, 1327
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 1 cut(s) 1953
Psp124BI GAGCTC 1 cut(s) 1676
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 8 cut(s) 333, 577, 608, 624, 974, 1167, 1471, 1918
PspCI CACGTG 2 cut(s) 169, 1327
PspEI GGTNACC 1 cut(s) 1539
PspFI CCCAGC 1 cut(s) 1132
PspGI CCWGG 8 cut(s) 333, 577, 608, 624, 974, 1167, 1471, 1918
PspN4I GGNNCC 8 cut(s) 115, 316, 317, 373, 495, 1126, 1917, 1972
PspPPI RGGWCCY 1 cut(s) 315
PsrI GAACNNNNNNTAC 2 cut(s) 335, 367
PstNI CAGNNNCTG 3 cut(s) 763, 1006, 1772
PsyI GACNNNGTC 1 cut(s) 335
RigI GGCCGGCC 1 cut(s) 657
RruI TCGCGA 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 801
Rsr2I CGGWCCG 1 cut(s) 57
RsrII CGGWCCG 1 cut(s) 57
SacI GAGCTC 1 cut(s) 1676
SacII CCGCGG 1 cut(s) 1410
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 9, 531, 1089, 1758
Sau3AI GATC 9 cut(s) 733, 810, 990, 1212, 1303, 1320, 1774, 2130, 2137
SchI GAGTC 5 cut(s) 135, 277, 628, 906, 1111
SduI GDGCHC 4 cut(s) 608, 1438, 1530, 1676
SexAI ACCWGGT 1 cut(s) 333
SfaNI GCATC 4 cut(s) 1168, 1231, 1456, 2120
SfcI CTRYAG 1 cut(s) 755
SfiI GGCCNNNNNGGCC 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 1917
Sfr274I CTCGAG 1 cut(s) 278
Sfr303I CCGCGG 1 cut(s) 1410
SgrAI CRCCGGYG 2 cut(s) 674, 728
SgrBI CCGCGG 1 cut(s) 1410
SinI GGWCC 7 cut(s) 57, 113, 315, 331, 493, 535, 1448
SlaI CTCGAG 1 cut(s) 278
SmaI CCCGGG 2 cut(s) 1559, 1586
SmiMI CAYNNNNRTG 1 cut(s) 801
SmlI CTYRAG 3 cut(s) 278, 659, 1984
SmoI CTYRAG 3 cut(s) 278, 659, 1984
SpeI ACTAGT 1 cut(s) 2170
SphI GCATGC 1 cut(s) 1224
Sse9I AATT 3 cut(s) 287, 1195, 1651
SspDI GGCGCC 1 cut(s) 1915
SspMI CTAG 4 cut(s) 384, 747, 1431, 2171
SstI GAGCTC 1 cut(s) 1676
StyI CCWWGG 1 cut(s) 1657
TaaI ACNGT 3 cut(s) 61, 1253, 1894
TasI AATT 3 cut(s) 287, 1195, 1651
TatI WGTACW 6 cut(s) 1032, 1248, 1626, 1647, 1956, 2164
TauI GCSGC 8 cut(s) 258, 267, 435, 455, 688, 1334, 1412, 1740
TfiI GAWTC 3 cut(s) 582, 771, 2105
Tru1I TTAA 4 cut(s) 9, 531, 1089, 1758
Tru9I TTAA 4 cut(s) 9, 531, 1089, 1758
TscAI CASTG 5 cut(s) 1109, 1579, 1683, 1777, 1897
TseFI GTSAC 7 cut(s) 169, 560, 631, 1742, 1790, 1828, 2122
TseI GCWGC 6 cut(s) 156, 159, 1372, 1664, 1766, 1847
Tsp45I GTSAC 7 cut(s) 169, 560, 631, 1742, 1790, 1828, 2122
TspDTI ATGAA 2 cut(s) 196, 1634
TspGWI ACGGA 4 cut(s) 506, 527, 712, 998
TspMI CCCGGG 2 cut(s) 1557, 1584
TspRI CASTG 5 cut(s) 1109, 1579, 1683, 1777, 1897
Tth111I GACNNNGTC 1 cut(s) 335
Van91I CCANNNNNTGG 2 cut(s) 250, 1174
VneI GTGCAC 1 cut(s) 604
VpaK11BI GGWCC 7 cut(s) 57, 113, 315, 331, 493, 535, 1448
XagI CCTNNNNNAGG 2 cut(s) 326, 1757
XapI RAATTY 1 cut(s) 1195
XceI RCATGY 2 cut(s) 1224, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 1909
XhoI CTCGAG 1 cut(s) 278
XmaI CCCGGG 2 cut(s) 1557, 1584
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 1694
XspI CTAG 4 cut(s) 384, 747, 1431, 2171
ZraI GACGTC 2 cut(s) 271, 1747
Zsp2I ATGCAT 2 cut(s) 1226, 1696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.