MD02G1026200.v1.1

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009084
Physical Location & Seq
Forward (+)
1980657 .. 1981937
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1026200.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGACGATCAAACCGGCGGTTCGAATCTCGGAACGGAAGCTGATCGTGAAGGACCGGACGATTCTGACGGGATTGCCGGACAATGTAGTGGCCACGTCCGGTTCGTCTTCCGGTCCGGTCGACGGGGTCTTCCTCGGCGCGAATTTCTCGGAGGAGAAAAGCCGGCACGTGGTTTCGTTAGGGACGCTTACCGGAGTCCGGTTCATGGCGTGCTTCCGGTTCAAGCTGTGGTGGATGGCCCAGAAGATGGGGGATCAGGGAAGGGACATACCTCTTGAGACTCAATTCCTATTGCTCGAGACCAAGCACGGGTCCCACCTGGAATCGGACGGCGGAGACGAAGAGAACCAGATCGTCTACACCGTGTTCCTCCCTCTCATCGAAGGCTCGTTCCGCGGTTGCCTGCAGGGCAACGCGAGCGACGAGCTCGAGCTCTGTTTGGAGAGCGGCGACGCCGACACCAAAGCGTCGTCGTTTTCCCACGCACTGCACGTCCATGCCGGGACGGACCCGTTCGGTACGATAACGGAAGCGATCAGGTCCGTCAAAGTTCACCTTCAGACGTTTCGTCAACGTCACGAGAAGAAACTCCCCGGATTCGTTGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCGGGACGTGACTCAGGAGGACGTGGAGGCCGGGCTCGAGTCTTTAGCCGCCGGTGGAACACCTCCGAAGTTCGTTATCATCGACGATGGGTGGCAGTCCGTCGGCGGCGACGACGGCGTTGAAAAGCAGGAGCCGCTAAGGCTGACTGGGATCAAAGAAAATTCAAAGTTTCAGAAGAAGGACGATCCGACAGTGGGGATTAAGAACATCGTCAGCATCGCGAAACAAAAGCACGGGTTGAAGTATGTGTACGTGTGGCACGCGATTACTGGCTACTGGGGCGGGGTCAGACCCGGAATCAAAGAGATGGAGGAATTCGGATCCTTGATGAAGTACCCGAACGTTTCCAGCGGTGTTGTGGCCAACGAGCCGACGTGGAAGACGGACGCGATGGCGGTGCGAGGGTTGGGGCTGGTGGATCCGAAGGGCGTGTACAAATTCTACAACGAATTGCACAGCTACTTGAGCTCTGCGGGTATTGACGGCGTTAAGGTGGACGTGCAGTGCATACTGGAAAACTCTCGGAGCCGGGCTAGGCGGTCGGGTCGAGTTGACCCAGAAGTACCACCAGGCGCTGGACGCCTCAGTGGCGAGGAATTTCCCGGACAACGGCTGCATCGCGTGCATGAGCCACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

427

Amino Acids

47.09

Weight (kDa)

6.14

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 398 6e-163 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 247, 1214
AccBSI CCGCTC 1 cut(s) 447
AccI GTMKAC 2 cut(s) 120, 357
AccII CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
AclI AACGTT 1 cut(s) 981
AclWI GGATC 7 cut(s) 261, 797, 818, 954, 967, 1052, 1065
AcoI YGGCCR 2 cut(s) 90, 999
AcsI RAATTY 5 cut(s) 142, 799, 953, 1076, 1235
AcuI CTGAAG 1 cut(s) 542
AcvI CACGTG 1 cut(s) 169
AcyI GRCGYC 2 cut(s) 453, 1219
AfaI GTAC 5 cut(s) 520, 890, 974, 1073, 1203
AflIII ACRYGT 2 cut(s) 630, 891
AgsI TTSAA 4 cut(s) 223, 761, 804, 880
AhdI GACNNNNNGTC 1 cut(s) 567
AjiI CACGTC 6 cut(s) 96, 493, 646, 661, 1014, 1138
AjnI CCWGG 3 cut(s) 318, 623, 1207
AluBI AGCT 6 cut(s) 40, 226, 427, 433, 1098, 1107
AluI AGCT 6 cut(s) 40, 226, 427, 433, 1098, 1107
Alw21I GWGCWC 4 cut(s) 429, 435, 623, 1109
Alw26I GTCTC 3 cut(s) 272, 293, 330
Alw44I GTGCAC 1 cut(s) 619
AlwI GGATC 7 cut(s) 261, 797, 818, 954, 967, 1052, 1065
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 3 cut(s) 296, 428, 674
AoxI GGCC 4 cut(s) 90, 237, 666, 999
ApaLI GTGCAC 1 cut(s) 619
ApeKI GCWGC 1 cut(s) 1252
ApoI RAATTY 5 cut(s) 142, 799, 953, 1076, 1235
AspLEI GCGC 2 cut(s) 140, 1214
AspS9I GGNCC 6 cut(s) 52, 113, 238, 312, 508, 540
AsuC2I CCSGG 7 cut(s) 502, 594, 641, 670, 933, 1169, 1242
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 1 cut(s) 22
AvaI CYCGRG 3 cut(s) 296, 428, 674
AvaII GGWCC 5 cut(s) 52, 113, 312, 508, 540
BaeGI GKGCMC 1 cut(s) 623
BalI TGGCCA 2 cut(s) 92, 1001
BamHI GGATCC 2 cut(s) 959, 1057
BanII GRGCYC 4 cut(s) 429, 435, 675, 1109
BarI GAAGNNNNNNTAC 4 cut(s) 872, 904, 1055, 1087
BauI CACGAG 1 cut(s) 578
BbrPI CACGTG 1 cut(s) 169
BbsI GAAGAC 3 cut(s) 99, 121, 1025
Bbv12I GWGCWC 4 cut(s) 429, 435, 623, 1109
BbvI GCAGC 1 cut(s) 1239
BccI CCATC 5 cut(s) 229, 241, 719, 940, 1024
BceAI ACGGC 4 cut(s) 346, 769, 1138, 1265
BcgI CGANNNNNNTGC 2 cut(s) 1018, 1052
BciT130I CCWGG 3 cut(s) 320, 625, 1209
BcnI CCSGG 7 cut(s) 502, 594, 641, 670, 933, 1169, 1242
BcoDI GTCTC 3 cut(s) 272, 293, 330
BfaI CTAG 1 cut(s) 1173
BfmI CTRYAG 1 cut(s) 404
BfoI RGCGCY 1 cut(s) 1215
BglI GCCNNNNNGGC 3 cut(s) 408, 778, 1227
BisI GCNGC 5 cut(s) 448, 687, 745, 773, 1253
BlsI GCNGC 5 cut(s) 449, 688, 746, 774, 1254
Bme18I GGWCC 5 cut(s) 52, 113, 312, 508, 540
BmeRI GACNNNNNGTC 1 cut(s) 567
BmeT110I CYCGRG 3 cut(s) 296, 428, 674
BmgBI CACGTC 6 cut(s) 96, 493, 646, 661, 1014, 1138
BmgT120I GGNCC 6 cut(s) 52, 113, 238, 312, 508, 540
BmiI GGNNCC 7 cut(s) 313, 314, 510, 771, 961, 1059, 1166
BmrI ACTGGG 2 cut(s) 795, 925
BmsI GCATC 2 cut(s) 864, 1264
BmuI ACTGGG 2 cut(s) 795, 925
BpiI GAAGAC 3 cut(s) 99, 121, 1025
Bpu10I CCTNAGC 1 cut(s) 776
Bpu14I TTCGAA 1 cut(s) 22
BpuEI CTTGAG 2 cut(s) 296, 1123
BpuMI CCSGG 7 cut(s) 502, 594, 641, 670, 933, 1169, 1242
BsaAI YACGTR 2 cut(s) 169, 892
BsaHI GRCGYC 2 cut(s) 453, 1219
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 133, 394, 592, 624
BsaWI WCCGGW 7 cut(s) 54, 98, 110, 115, 191, 198, 216
Bse118I RCCGGY 3 cut(s) 13, 162, 689
Bse1I ACTGG 4 cut(s) 790, 913, 920, 1155
BseBI CCWGG 3 cut(s) 320, 625, 1209
BseDI CCNNGG 4 cut(s) 133, 394, 592, 624
BseGI GGATG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 665, 1237
BseNI ACTGG 4 cut(s) 790, 913, 920, 1155
BseRI GAGGAG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 623
BseXI GCAGC 1 cut(s) 1239
BsgI GTGCAG 2 cut(s) 473, 1160
Bsh1236I CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
Bsh1285I CGRYCG 2 cut(s) 120, 1181
BshFI GGCC 4 cut(s) 92, 239, 668, 1001
BsiEI CGRYCG 2 cut(s) 120, 1181
BsiHKAI GWGCWC 4 cut(s) 429, 435, 623, 1109
BsiHKCI CYCGRG 3 cut(s) 296, 428, 674
BslFI GGGAC 6 cut(s) 196, 278, 298, 517, 641, 656
BsmAI GTCTC 3 cut(s) 272, 293, 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 6 cut(s) 196, 278, 298, 517, 641, 656
BsnI GGCC 4 cut(s) 92, 239, 668, 1001
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 3 cut(s) 296, 428, 674
Bsp119I TTCGAA 1 cut(s) 22
Bsp1286I GDGCHC 5 cut(s) 429, 435, 623, 675, 1109
Bsp1407I TGTACA 1 cut(s) 1071
Bsp143I GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
Bsp68I TCGCGA 1 cut(s) 860
BspANI GGCC 4 cut(s) 92, 239, 668, 1001
BspCNI CTCAG 2 cut(s) 664, 1236
BspFNI CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
BspLI GGNNCC 7 cut(s) 313, 314, 510, 771, 961, 1059, 1166
BspMAI CTGCAG 1 cut(s) 408
BspPI GGATC 7 cut(s) 261, 797, 818, 954, 967, 1052, 1065
BspT104I TTCGAA 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 447
BsrFI RCCGGY 3 cut(s) 13, 162, 689
BsrGI TGTACA 1 cut(s) 1071
BsrI ACTGG 4 cut(s) 790, 913, 920, 1155
BssAI RCCGGY 3 cut(s) 13, 162, 689
BssECI CCNNGG 4 cut(s) 133, 394, 592, 624
BssMI GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
BssNI GRCGYC 2 cut(s) 453, 1219
BssSI CACGAG 1 cut(s) 578
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 3 cut(s) 320, 625, 1209
Bst4CI ACNGT 2 cut(s) 364, 832
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 2 cut(s) 453, 1219
BstAPI GCANNNNNTGC 1 cut(s) 1261
BstAUI TGTACA 1 cut(s) 1071
BstBAI YACGTR 2 cut(s) 169, 892
BstBI TTCGAA 1 cut(s) 22
BstC8I GCNNGC 6 cut(s) 164, 211, 404, 418, 900, 1262
BstDEI CTNAG 3 cut(s) 651, 776, 1223
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
BstH2I RGCGCY 1 cut(s) 1215
BstHHI GCGC 2 cut(s) 140, 1214
BstKTI GATC 9 cut(s) 9, 45, 256, 354, 537, 792, 826, 962, 1060
BstMAI GTCTC 3 cut(s) 272, 293, 330
BstMBI GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
BstMCI CGRYCG 2 cut(s) 120, 1181
BstNI CCWGG 3 cut(s) 320, 625, 1209
BstSFI CTRYAG 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 623
BstUI CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
BstV1I GCAGC 1 cut(s) 1239
BstV2I GAAGAC 3 cut(s) 99, 121, 1025
BstX2I RGATCY 2 cut(s) 959, 1057
BstYI RGATCY 2 cut(s) 959, 1057
BsuRI GGCC 4 cut(s) 92, 239, 668, 1001
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 3 cut(s) 841, 1043, 1241
BtrI CACGTC 6 cut(s) 96, 493, 646, 661, 1014, 1138
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 485, 1148
BtsIMutI CAGTG 4 cut(s) 485, 837, 1148, 1231
BtuMI TCGCGA 1 cut(s) 860
Cac8I GCNNGC 6 cut(s) 164, 211, 404, 418, 900, 1262
CaiI CAGNNNCTG 1 cut(s) 1214
CfoI GCGC 2 cut(s) 140, 1214
Cfr10I RCCGGY 3 cut(s) 13, 162, 689
Cfr13I GGNCC 6 cut(s) 52, 113, 238, 312, 508, 540
Cfr42I CCGCGG 1 cut(s) 397
CpoI CGGWCCG 1 cut(s) 113
CseI GACGC 6 cut(s) 193, 456, 461, 638, 1034, 1227
Csp6I GTAC 5 cut(s) 519, 889, 973, 1072, 1202
CspI CGGWCCG 1 cut(s) 113
CviAII CATG 3 cut(s) 205, 497, 1265
CviQI GTAC 5 cut(s) 519, 889, 973, 1072, 1202
DdeI CTNAG 3 cut(s) 651, 776, 1223
DpnI GATC 9 cut(s) 8, 44, 255, 353, 536, 791, 825, 961, 1059
DpnII GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
DriI GACNNNNNGTC 1 cut(s) 567
EaeI YGGCCR 2 cut(s) 90, 999
Eam1104I CTCTTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 348
Ecl136II GAGCTC 3 cut(s) 427, 433, 1107
Eco24I GRGCYC 4 cut(s) 429, 435, 675, 1109
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 5 cut(s) 52, 113, 312, 508, 540
Eco53kI GAGCTC 3 cut(s) 427, 433, 1107
Eco57I CTGAAG 1 cut(s) 542
Eco72I CACGTG 1 cut(s) 169
Eco88I CYCGRG 3 cut(s) 296, 428, 674
EcoICRI GAGCTC 3 cut(s) 427, 433, 1107
EcoO109I RGGNCCY 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 953
EcoRII CCWGG 3 cut(s) 318, 623, 1207
EcoT38I GRGCYC 4 cut(s) 429, 435, 675, 1109
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 3 cut(s) 208, 500, 1268
FaiI YATR 6 cut(s) 206, 269, 498, 885, 1148, 1266
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 6 cut(s) 196, 278, 298, 517, 641, 656
FatI CATG 3 cut(s) 204, 496, 1264
FauI CCCGC 2 cut(s) 914, 1105
FblI GTMKAC 2 cut(s) 120, 357
Fnu4HI GCNGC 5 cut(s) 448, 687, 745, 773, 1253
FokI GGATG 1 cut(s) 247
FriOI GRGCYC 4 cut(s) 429, 435, 675, 1109
Fsp4HI GCNGC 5 cut(s) 448, 687, 745, 773, 1253
FspBI CTAG 1 cut(s) 1173
GlaI GCGC 2 cut(s) 139, 1213
GluI GCNGC 5 cut(s) 448, 687, 745, 773, 1253
HaeII RGCGCY 1 cut(s) 1215
HaeIII GGCC 4 cut(s) 92, 239, 668, 1001
HgaI GACGC 6 cut(s) 193, 456, 461, 638, 1034, 1227
HhaI GCGC 2 cut(s) 140, 1214
Hin1I GRCGYC 2 cut(s) 453, 1219
Hin1II CATG 3 cut(s) 208, 500, 1268
Hin6I GCGC 2 cut(s) 138, 1212
HinP1I GCGC 2 cut(s) 138, 1212
HincII GTYRAC 4 cut(s) 121, 572, 604, 1192
HindII GTYRAC 4 cut(s) 121, 572, 604, 1192
HinfI GANTC 9 cut(s) 24, 61, 195, 280, 323, 597, 649, 677, 936
HphI GGTGA 1 cut(s) 545
Hpy188III TCNNGA 6 cut(s) 46, 275, 298, 578, 653, 859
HpyAV CCTTC 6 cut(s) 43, 255, 377, 566, 811, 1057
HpyCH4III ACNGT 2 cut(s) 364, 832
HpyCH4V TGCA 8 cut(s) 406, 490, 621, 1093, 1141, 1146, 1255, 1264
HpyF3I CTNAG 3 cut(s) 651, 776, 1223
Hsp92I GRCGYC 2 cut(s) 453, 1219
Hsp92II CATG 3 cut(s) 208, 500, 1268
HspAI GCGC 2 cut(s) 138, 1212
KflI GGGWCCC 1 cut(s) 312
KroI GCCGGC 1 cut(s) 162
KroNI GCCGGC 1 cut(s) 164
KspI CCGCGG 1 cut(s) 397
Kzo9I GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
LmnI GCTCC 2 cut(s) 769, 1164
Lsp1109I GCAGC 1 cut(s) 1239
LweI GCATC 2 cut(s) 864, 1264
MaeI CTAG 1 cut(s) 1173
MaeIII GTNAC 2 cut(s) 575, 646
MalI GATC 9 cut(s) 8, 44, 255, 353, 536, 791, 825, 961, 1059
MbiI CCGCTC 1 cut(s) 447
MboI GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
MboII GAAGA 7 cut(s) 99, 121, 256, 353, 595, 826, 1030
MflI RGATCY 2 cut(s) 959, 1057
MhlI GDGCHC 5 cut(s) 429, 435, 623, 675, 1109
MlsI TGGCCA 2 cut(s) 92, 1001
MluCI AATT 7 cut(s) 142, 284, 799, 953, 1076, 1088, 1235
MluI ACGCGT 1 cut(s) 630
MluNI TGGCCA 2 cut(s) 92, 1001
MlyI GAGTC 4 cut(s) 204, 274, 643, 686
MmeI TCCRAC 1 cut(s) 851
Mox20I TGGCCA 2 cut(s) 92, 1001
MroNI GCCGGC 1 cut(s) 162
MscI TGGCCA 2 cut(s) 92, 1001
MseI TTAA 2 cut(s) 840, 1128
MslI CAYNNNNRTG 1 cut(s) 495
Msp20I TGGCCA 2 cut(s) 92, 1001
MspA1I CMGCKG 2 cut(s) 396, 990
MvaI CCWGG 3 cut(s) 320, 625, 1209
MvnI CGCG 8 cut(s) 140, 396, 416, 632, 860, 902, 1028, 1260
NaeI GCCGGC 1 cut(s) 164
NciI CCSGG 7 cut(s) 502, 594, 641, 670, 933, 1169, 1242
NdeII GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
NgoMIV GCCGGC 1 cut(s) 162
NlaIII CATG 3 cut(s) 208, 500, 1268
NlaIV GGNNCC 7 cut(s) 313, 314, 510, 771, 961, 1059, 1166
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 575, 646
NruI TCGCGA 1 cut(s) 860
NspV TTCGAA 1 cut(s) 22
PaeR7I CTCGAG 3 cut(s) 296, 428, 674
PcsI WCGNNNNNNNCGW 7 cut(s) 101, 336, 360, 420, 489, 717, 747
PdiI GCCGGC 1 cut(s) 164
PfeI GAWTC 5 cut(s) 24, 61, 323, 597, 936
PflFI GACNNNGTC 1 cut(s) 125
PflMI CCANNNNNTGG 2 cut(s) 247, 1214
PfoI TCCNGGA 1 cut(s) 1240
PkrI GCNGC 5 cut(s) 449, 688, 746, 774, 1254
PleI GAGTC 4 cut(s) 203, 274, 643, 685
PmaCI CACGTG 1 cut(s) 169
PmlI CACGTG 1 cut(s) 169
PpsI GAGTC 4 cut(s) 203, 274, 643, 685
Ppu21I YACGTR 2 cut(s) 169, 892
PpuMI RGGWCCY 1 cut(s) 312
Psp124BI GAGCTC 3 cut(s) 429, 435, 1109
Psp1406I AACGTT 1 cut(s) 981
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 3 cut(s) 318, 623, 1207
PspCI CACGTG 1 cut(s) 169
PspGI CCWGG 3 cut(s) 318, 623, 1207
PspN4I GGNNCC 7 cut(s) 313, 314, 510, 771, 961, 1059, 1166
PspPI GGNCC 6 cut(s) 52, 113, 238, 312, 508, 540
PspPPI RGGWCCY 1 cut(s) 312
PspXI VCTCGAGB 2 cut(s) 428, 674
PstI CTGCAG 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 2 cut(s) 959, 1057
PsyI GACNNNGTC 1 cut(s) 125
RruI TCGCGA 1 cut(s) 860
RsaI GTAC 5 cut(s) 520, 890, 974, 1073, 1203
RsaNI GTAC 5 cut(s) 519, 889, 973, 1072, 1202
RseI CAYNNNNRTG 1 cut(s) 495
Rsr2I CGGWCCG 1 cut(s) 113
RsrII CGGWCCG 1 cut(s) 113
SacI GAGCTC 3 cut(s) 429, 435, 1109
SacII CCGCGG 1 cut(s) 397
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 840, 1128
SatI GCNGC 5 cut(s) 448, 687, 745, 773, 1253
Sau3AI GATC 9 cut(s) 6, 42, 253, 351, 534, 789, 823, 959, 1057
Sau96I GGNCC 6 cut(s) 52, 113, 238, 312, 508, 540
SbfI CCTGCAGG 1 cut(s) 408
SchI GAGTC 4 cut(s) 204, 274, 643, 686
SdaI CCTGCAGG 1 cut(s) 408
SduI GDGCHC 5 cut(s) 429, 435, 623, 675, 1109
SfaNI GCATC 2 cut(s) 864, 1264
SfcI CTRYAG 1 cut(s) 404
Sfr274I CTCGAG 3 cut(s) 296, 428, 674
Sfr303I CCGCGG 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 5 cut(s) 52, 113, 312, 508, 540
SlaI CTCGAG 3 cut(s) 296, 428, 674
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 5 cut(s) 275, 296, 428, 674, 1102
SmoI CTYRAG 5 cut(s) 275, 296, 428, 674, 1102
Sse8387I CCTGCAGG 1 cut(s) 408
Sse9I AATT 7 cut(s) 142, 284, 799, 953, 1076, 1088, 1235
SspMI CTAG 1 cut(s) 1173
SstI GAGCTC 3 cut(s) 429, 435, 1109
TaaI ACNGT 2 cut(s) 364, 832
TaqI TCGA 8 cut(s) 22, 120, 297, 381, 429, 675, 720, 1186
TasI AATT 7 cut(s) 142, 284, 799, 953, 1076, 1088, 1235
TatI WGTACW 1 cut(s) 1071
TauI GCSGC 4 cut(s) 450, 689, 747, 775
TfiI GAWTC 5 cut(s) 24, 61, 323, 597, 936
Tru1I TTAA 2 cut(s) 840, 1128
Tru9I TTAA 2 cut(s) 840, 1128
TscAI CASTG 4 cut(s) 492, 837, 1148, 1231
TseFI GTSAC 2 cut(s) 575, 646
TseI GCWGC 1 cut(s) 1252
Tsp45I GTSAC 2 cut(s) 575, 646
TspDTI ATGAA 2 cut(s) 193, 983
TspGWI ACGGA 6 cut(s) 49, 521, 532, 542, 727, 1037
TspRI CASTG 4 cut(s) 492, 837, 1148, 1231
Tth111I GACNNNGTC 1 cut(s) 125
Van91I CCANNNNNTGG 2 cut(s) 247, 1214
VneI GTGCAC 1 cut(s) 619
VpaK11BI GGWCC 5 cut(s) 52, 113, 312, 508, 540
XapI RAATTY 5 cut(s) 142, 799, 953, 1076, 1235
XcmI CCANNNNNNNNNTGG 1 cut(s) 994
XhoI CTCGAG 3 cut(s) 296, 428, 674
XmiI GTMKAC 2 cut(s) 120, 357
XspI CTAG 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.