Rw2G002450

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009084
Physical Location & Seq
Forward (+)
2039649 .. 2042623
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002450.1

Sequence Viewer

Length: 2370 bp
ATGGTGTCCAATCTGTGCTACAGGTCTCCTCCTCTCACTCGTCTTATAAATACCTCTCGCCCAATTCATCTTCCCAAATCAAACCTTCCTTCTCCAATTCATCTTCCCAAATCAAACCTTTTTTCTCCACTCCGGTTGACGCATACTCATTCGACTTCTGTGAAATTTCAGAGAACCCAGAAGCCTTTACATACCAGAAAGGACAGCGACAGCCCGATGACGATTAAGCCGGCGGTTAGCGTTTCCGACGGGAAACTGATAGTGAAGAACCGGACCGTTCTGACCGGATTACCGGACAATGTGGGAGTCTTCCTCGGCGCGGCGTTCGAGGAGGGCAGCAGCCGGCACGTGACTTCTCTCGGAACTTTTCAAAACGGCGTGCGTTTCATGGCGTGCTTCCGGTTCAAGCTATGGTGGATGGCCCAGAAAATGGGCCGCTCCGGCCGCGACGTCCCTCTCGAGACTCAATTCCTATTGGTCGAGACCCAAGACGGGTCCCACCTCGAAGAGGACCAGGTCGTGTACGCCGTGTTCCTCCCTCTTATCGAAGGCCCCTTCAGGGCTAGCTTACAGGGGAACAGCGGAGACGAGCTGGAGCTTTGCTTGGAGAGCGGCGACGTGGACACCAAAGCGGCGTCGTTTAGCCACGCGGTGTTCGTTCACGCCGGGACGGACCCGTTCGCGACGATAACGGAGGCGGTCAGGGCCGTTAAGGTCCACCTGAAGACGTTTCGTCAACGTCACGAGAAGAAGCTCCCTGGAATCGTCGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCAGCAAGTGACTCAGGAAGGAGTTGAGGCCGGCCTCAAGTCCCTCACCGCCGGCGGAGCGCCGCCGAAGTTTGTCATCATCGACGACGGGTGGCAGTCCGTCGCCGGCGATCAGGACAAAGCCGGCGAGCTACAGAGACTGACCGGAATCAAAGAAAACTCGAAGTTTCAACACAAGGATGATGGGATCATGACGATAGTGAACATTGCAAAAGAAAAATACGGGTTGAAGTACGTGTACGTCTGGCACGCGATTACCGGCTACTGGGGAGGAGTCCGGCCCGGAACTGAATACGGGTCGGTTCTGAAGTACCCGGATTTGTCGAAAGGGGTTATGGAAAACGAACCGGCCTGGAAAACGGACATGATCCGGTTACAGGGTCTGGGCTTAGTGAACCCGAAAAGCGTGTACAAGTTTTACAACGAGCTACACAGCTACTTGGCCTCTGCGGGTGTAGATGGGGTTAAGGTGGACGCGCAGTGCGTGCTGGAGACTCTCGGGGCCGGGCTGGGCGGGCGGGTCGAGCTGACCCGGCAGTACCACCAGGCATTGGATGCTTCGGTGTCTCGGAACTTTGAAGATAATGGGATCATAGCATGCATGAGCCATAACACTGATGCGCTGTACTGTGAGAAACAGACGGCGGTGGTGAGAGCCTCCGACGATTTCTACCCGCGCGATCCGATGTCGCATACGATCCACGTGGCGGCGGTGGCGTATAATAGCGTTTTCCTCGGCGAGTTTATGCTGCCGGATTGGGATATGTTCCACTCGCTCCACCCCGCGGCTGAGTACCACGCGTCCGCTAGAGCCATTAGTGGTGGACCTGTTTATGTTAGTGATGCGCCTGGGAAGCATGACTTTGAGCTTTTGAAGAAGATAGTATTGCCGGACGGGTCGGTGCTCCGGGCGCGGTTACCCGGAAGGCCGACCCGGGACTGCTTATTCACTGACCCGGCCCGGGATGGGGTGAGTTTGTTGAAGATATGGAACATGAATAAGTACACAGGTGTAGTGGGGGTGTACAATTGCCAAGGAGCAGCATGGAGCTCACTGGAGAGAAAGAATGCATTCCACGAGACGAAATCGGACGCCATTACCGGTTATGTTCGCGGCCGTGACGTCCACCTCATTAAGGAGGCAGCCACAGATCCCGATTGGAATGGTGACTGCGCCGTGTATTGCAATCGGACAGGGGAGCTTGTGACATTGCCTCACAATGCTGCCATGCCTATTTCCCTCAAAGTGCTTGAACATGAGATTTTCACTGTCAGCCCCATCAGGGTTTTGGCGCCTGGCGTGAGATTCGCGCCTTTGGGACTGGTTGACATGTACAATGCCGGTGGAGCCATTGAAGACTTGAGATATGAGCAGAGATTAGTCTCTATGGAGGTTAGAGGGTGTGGCAAGTTTGGTGTGTATTCTTCAGAGAAGCCCATGAGGTGCTGTGTAGGGACTAATGGGGTTGATTTCAGCTATGATTCGGCATCTGGGTTGGTGACTTTGATCTTGGATCATATGCCTGAAGAGGGAAAAAGAGTACAACTAGTCGAGGTTGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

789

Amino Acids

87.22

Weight (kDa)

6.63

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 94 - 770 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AatII GACGTC 2 cut(s) 453, 1929
AccB1I GGYRCC 1 cut(s) 2095
AccB7I CCANNNNNTGG 2 cut(s) 430, 1354
AccBSI CCGCTC 2 cut(s) 438, 612
AccI GTMKAC 1 cut(s) 768
AclWI GGATC 7 cut(s) 998, 1165, 1400, 1478, 1495, 1949, 2325
AcoI YGGCCR 2 cut(s) 442, 1918
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 5 cut(s) 541, 743, 1130, 2214, 2349
AcvI CACGTG 2 cut(s) 349, 1507
AcyI GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
AdeI CACNNNGTG 1 cut(s) 652
AflIII ACRYGT 4 cut(s) 795, 1038, 1602, 2133
AgeI ACCGGT 1 cut(s) 1904
AgsI TTSAA 9 cut(s) 371, 406, 974, 1033, 1382, 1678, 1786, 2057, 2159
AhdI GACNNNNNGTC 1 cut(s) 732
AhlI ACTAGT 1 cut(s) 2350
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 7 cut(s) 513, 757, 788, 1154, 1347, 1651, 2098
Alw21I GWGCWC 3 cut(s) 788, 1710, 1856
Alw26I GTCTC 9 cut(s) 30, 455, 476, 579, 934, 1289, 1374, 1877, 2191
Alw44I GTGCAC 1 cut(s) 784
AlwI GGATC 7 cut(s) 998, 1165, 1400, 1478, 1495, 1949, 2325
AlwNI CAGNNNCTG 2 cut(s) 943, 1186
Ama87I CYCGRG 4 cut(s) 458, 1301, 1737, 1764
ApaLI GTGCAC 1 cut(s) 784
ApeKI GCWGC 6 cut(s) 336, 339, 1552, 1844, 1946, 2027
ApoI RAATTY 1 cut(s) 164
AsiGI ACCGGT 1 cut(s) 1904
Asp700I GAANNNNTTC 1 cut(s) 1874
AsuHPI GGTGA 5 cut(s) 841, 1465, 1786, 1982, 2314
AsuNHI GCTAGC 1 cut(s) 563
AvaI CYCGRG 4 cut(s) 458, 1301, 1737, 1764
AvaII GGWCC 6 cut(s) 273, 495, 511, 673, 715, 1628
BaeGI GKGCMC 1 cut(s) 788
BanI GGYRCC 1 cut(s) 2095
BanII GRGCYC 1 cut(s) 1856
BarI GAAGNNNNNNTAC 2 cut(s) 1025, 1057
BauI CACGAG 2 cut(s) 743, 1880
BbrPI CACGTG 2 cut(s) 349, 1507
BbsI GAAGAC 3 cut(s) 301, 731, 2166
Bbv12I GWGCWC 3 cut(s) 788, 1710, 1856
BbvI GCAGC 6 cut(s) 348, 351, 1539, 1856, 1958, 2014
BccI CCATC 5 cut(s) 412, 980, 1256, 1763, 2090
BceAI ACGGC 6 cut(s) 391, 512, 692, 1461, 1905, 1964
BciT130I CCWGG 7 cut(s) 515, 759, 790, 1156, 1349, 1653, 2100
BcoDI GTCTC 9 cut(s) 30, 455, 476, 579, 934, 1289, 1374, 1877, 2191
BcuI ACTAGT 1 cut(s) 2350
BfaI CTAG 3 cut(s) 564, 1611, 2351
BfmI CTRYAG 2 cut(s) 19, 935
BfoI RGCGCY 2 cut(s) 866, 2099
BglI GCCNNNNNGGC 1 cut(s) 441
Bme18I GGWCC 6 cut(s) 273, 495, 511, 673, 715, 1628
BmeRI GACNNNNNGTC 1 cut(s) 732
BmeT110I CYCGRG 4 cut(s) 458, 1301, 1737, 1764
BmgBI CACGTC 1 cut(s) 619
BmiI GGNNCC 7 cut(s) 496, 497, 553, 675, 1306, 2097, 2152
BmrI ACTGGG 1 cut(s) 1078
BmsI GCATC 4 cut(s) 1348, 1411, 1636, 2300
BmtI GCTAGC 1 cut(s) 567
BmuI ACTGGG 1 cut(s) 1078
BpiI GAAGAC 3 cut(s) 301, 731, 2166
BplI GAGNNNNNCTC 2 cut(s) 297, 329
BpmI CTGGAG 3 cut(s) 614, 1313, 1880
BpuEI CTTGAG 2 cut(s) 824, 2185
BsaAI YACGTR 3 cut(s) 349, 1039, 1507
BsaHI GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
BsaI GGTCTC 2 cut(s) 30, 476
BsaJI CCNNGG 9 cut(s) 313, 757, 789, 1537, 1587, 1652, 1737, 1764, 1837
BsaWI WCCGGW 8 cut(s) 132, 270, 284, 292, 399, 947, 1173, 1904
Bse1I ACTGG 3 cut(s) 1073, 1863, 2130
Bse3DI GCAATG 2 cut(s) 1008, 2012
BseBI CCWGG 7 cut(s) 515, 759, 790, 1156, 1349, 1653, 2100
BseDI CCNNGG 9 cut(s) 313, 757, 789, 1537, 1587, 1652, 1737, 1764, 1837
BseGI GGATG 4 cut(s) 423, 988, 1363, 1774
BseMI GCAATG 2 cut(s) 1008, 2012
BseMII CTCAG 2 cut(s) 830, 1584
BseNI ACTGG 3 cut(s) 1073, 1863, 2130
BseRI GAGGAG 4 cut(s) 18, 21, 344, 1089
BseSI GKGCMC 1 cut(s) 788
BseX3I CGGCCG 2 cut(s) 442, 1918
BseXI GCAGC 6 cut(s) 348, 351, 1539, 1856, 1958, 2014
BseYI CCCAGC 1 cut(s) 1312
Bsh1285I CGRYCG 2 cut(s) 445, 1921
BshNI GGYRCC 1 cut(s) 2095
BshTI ACCGGT 1 cut(s) 1904
BsiEI CGRYCG 2 cut(s) 445, 1921
BsiHKAI GWGCWC 3 cut(s) 788, 1710, 1856
BsiHKCI CYCGRG 4 cut(s) 458, 1301, 1737, 1764
BslFI GGGAC 8 cut(s) 437, 481, 682, 806, 829, 1754, 2136, 2272
BsmAI GTCTC 9 cut(s) 30, 455, 476, 579, 934, 1289, 1374, 1877, 2191
BsmBI CGTCTC 2 cut(s) 579, 1877
BsmFI GGGAC 8 cut(s) 437, 481, 682, 806, 829, 1754, 2136, 2272
BsmI GAATGC 2 cut(s) 1874, 1876
Bso31I GGTCTC 2 cut(s) 30, 476
BsoBI CYCGRG 4 cut(s) 458, 1301, 1737, 1764
Bsp1286I GDGCHC 3 cut(s) 788, 1710, 1856
Bsp1407I TGTACA 3 cut(s) 1212, 1827, 2136
Bsp143I GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
Bsp68I TCGCGA 1 cut(s) 683
BspCNI CTCAG 2 cut(s) 829, 1585
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 7 cut(s) 496, 497, 553, 675, 1306, 2097, 2152
BspOI GCTAGC 1 cut(s) 567
BspPI GGATC 7 cut(s) 998, 1165, 1400, 1478, 1495, 1949, 2325
BspT107I GGYRCC 1 cut(s) 2095
BspTNI GGTCTC 2 cut(s) 30, 476
BsrBI CCGCTC 2 cut(s) 438, 612
BsrDI GCAATG 2 cut(s) 1008, 2012
BsrGI TGTACA 3 cut(s) 1212, 1827, 2136
BsrI ACTGG 3 cut(s) 1073, 1863, 2130
BssECI CCNNGG 9 cut(s) 313, 757, 789, 1537, 1587, 1652, 1737, 1764, 1837
BssMI GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
BssNI GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
BssSI CACGAG 2 cut(s) 743, 1880
BssT1I CCWWGG 1 cut(s) 1837
Bst2BI CACGAG 2 cut(s) 743, 1880
Bst2UI CCWGG 7 cut(s) 515, 759, 790, 1156, 1349, 1653, 2100
Bst4CI ACNGT 3 cut(s) 277, 1433, 2074
Bst6I CTCTTC 2 cut(s) 501, 2325
BstACI GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
BstAPI GCANNNNNTGC 2 cut(s) 1288, 1358
BstAUI TGTACA 3 cut(s) 1212, 1827, 2136
BstBAI YACGTR 3 cut(s) 349, 1039, 1507
BstDEI CTNAG 3 cut(s) 816, 1192, 1593
BstDSI CCRYGG 1 cut(s) 1587
BstEII GGTNACC 1 cut(s) 1719
BstENI CCTNNNNNAGG 2 cut(s) 506, 1937
BstF5I GGATG 4 cut(s) 423, 988, 1363, 1774
BstH2I RGCGCY 2 cut(s) 866, 2099
BstKTI GATC 9 cut(s) 916, 993, 1173, 1395, 1486, 1503, 1957, 2313, 2320
BstMAI GTCTC 9 cut(s) 30, 455, 476, 579, 934, 1289, 1374, 1877, 2191
BstMBI GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
BstMCI CGRYCG 2 cut(s) 445, 1921
BstNI CCWGG 7 cut(s) 515, 759, 790, 1156, 1349, 1653, 2100
BstNSI RCATGY 2 cut(s) 1404, 2137
BstPI GGTNACC 1 cut(s) 1719
BstSFI CTRYAG 2 cut(s) 19, 935
BstSLI GKGCMC 1 cut(s) 788
BstV1I GCAGC 6 cut(s) 348, 351, 1539, 1856, 1958, 2014
BstV2I GAAGAC 3 cut(s) 301, 731, 2166
BstX2I RGATCY 1 cut(s) 1954
BstYI RGATCY 1 cut(s) 1954
BstZI CGGCCG 2 cut(s) 442, 1918
BtgI CCRYGG 1 cut(s) 1587
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 4 cut(s) 423, 988, 1363, 1774
BtsI GCAGTG 1 cut(s) 1289
BtsIMutI CAGTG 5 cut(s) 1289, 1416, 1752, 1856, 2070
BtuMI TCGCGA 1 cut(s) 683
CaiI CAGNNNCTG 2 cut(s) 943, 1186
CciI TCATGA 1 cut(s) 993
Cfr42I CCGCGG 1 cut(s) 1590
Cfr9I CCCGGG 2 cut(s) 1737, 1764
CpoI CGGWCCG 1 cut(s) 273
CseI GACGC 6 cut(s) 148, 624, 803, 1286, 1593, 1904
CsiI ACCWGGT 1 cut(s) 513
CspAI ACCGGT 1 cut(s) 1904
CspCI CAANNNNNGTGG 4 cut(s) 117, 152, 2128, 2163
CspI CGGWCCG 1 cut(s) 273
DdeI CTNAG 3 cut(s) 816, 1192, 1593
DinI GGCGCC 1 cut(s) 2097
DpnI GATC 9 cut(s) 915, 992, 1172, 1394, 1485, 1502, 1956, 2312, 2319
DpnII GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
DraIII CACNNNGTG 1 cut(s) 652
DriI GACNNNNNGTC 1 cut(s) 732
EaeI YGGCCR 2 cut(s) 442, 1918
EagI CGGCCG 2 cut(s) 442, 1918
Eam1104I CTCTTC 2 cut(s) 501, 2325
Eam1105I GACNNNNNGTC 1 cut(s) 732
EarI CTCTTC 2 cut(s) 501, 2325
EciI GGCGGA 1 cut(s) 873
Ecl136II GAGCTC 1 cut(s) 1854
EclXI CGGCCG 2 cut(s) 442, 1918
Eco130I CCWWGG 1 cut(s) 1837
Eco24I GRGCYC 1 cut(s) 1856
Eco31I GGTCTC 2 cut(s) 30, 476
Eco47I GGWCC 6 cut(s) 273, 495, 511, 673, 715, 1628
Eco52I CGGCCG 2 cut(s) 442, 1918
Eco53kI GAGCTC 1 cut(s) 1854
Eco57I CTGAAG 5 cut(s) 541, 743, 1130, 2214, 2349
Eco72I CACGTG 2 cut(s) 349, 1507
Eco88I CYCGRG 4 cut(s) 458, 1301, 1737, 1764
Eco91I GGTNACC 1 cut(s) 1719
EcoICRI GAGCTC 1 cut(s) 1854
EcoNI CCTNNNNNAGG 2 cut(s) 506, 1937
EcoO109I RGGNCCY 2 cut(s) 495, 551
EcoO65I GGTNACC 1 cut(s) 1719
EcoRII CCWGG 7 cut(s) 513, 757, 788, 1154, 1347, 1651, 2098
EcoT14I CCWWGG 1 cut(s) 1837
EcoT22I ATGCAT 2 cut(s) 1406, 1876
EcoT38I GRGCYC 1 cut(s) 1856
EgeI GGCGCC 1 cut(s) 2097
EheI GGCGCC 1 cut(s) 2097
ErhI CCWWGG 1 cut(s) 1837
Esp3I CGTCTC 2 cut(s) 579, 1877
FalI AAGNNNNNCTT 2 cut(s) 1649, 1681
FaqI GGGAC 8 cut(s) 437, 481, 682, 806, 829, 1754, 2136, 2272
FauI CCCGC 5 cut(s) 1246, 1310, 1314, 1485, 1594
FauNDI CATATG 1 cut(s) 2322
FblI GTMKAC 1 cut(s) 768
FokI GGATG 4 cut(s) 430, 995, 1370, 1781
FriOI GRGCYC 1 cut(s) 1856
FseI GGCCGGCC 1 cut(s) 837
FspBI CTAG 3 cut(s) 564, 1611, 2351
GsaI CCCAGC 1 cut(s) 1316
GsuI CTGGAG 3 cut(s) 614, 1313, 1880
HaeII RGCGCY 2 cut(s) 866, 2099
HgaI GACGC 6 cut(s) 148, 624, 803, 1286, 1593, 1904
Hin1I GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
HincII GTYRAC 4 cut(s) 138, 737, 769, 2131
HindII GTYRAC 4 cut(s) 138, 737, 769, 2131
HinfI GANTC 9 cut(s) 306, 463, 762, 814, 951, 1077, 1297, 2109, 2285
HphI GGTGA 5 cut(s) 841, 1465, 1786, 1982, 2314
Hpy188III TCNNGA 9 cut(s) 458, 460, 481, 682, 743, 818, 917, 994, 1958
HpyAV CCTTC 6 cut(s) 95, 99, 542, 565, 815, 1722
HpyCH4III ACNGT 3 cut(s) 277, 1433, 2074
HpyCH4IV ACGT 9 cut(s) 348, 450, 618, 728, 739, 1038, 1044, 1506, 1926
HpyCH4V TGCA 5 cut(s) 786, 1013, 1404, 1874, 1989
HpyF3I CTNAG 3 cut(s) 816, 1192, 1593
HpySE526I ACGT 9 cut(s) 348, 450, 618, 728, 739, 1038, 1044, 1506, 1926
Hsp92I GRCGYC 5 cut(s) 450, 635, 1896, 1926, 2096
KasI GGCGCC 1 cut(s) 2095
KflI GGGWCCC 1 cut(s) 495
KroI GCCGGC 6 cut(s) 229, 342, 833, 854, 908, 926
KroNI GCCGGC 6 cut(s) 231, 344, 835, 856, 910, 928
KspI CCGCGG 1 cut(s) 1590
Kzo9I GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
Lsp1109I GCAGC 6 cut(s) 348, 351, 1539, 1856, 1958, 2014
LweI GCATC 4 cut(s) 1348, 1411, 1636, 2300
MabI ACCWGGT 1 cut(s) 513
MaeI CTAG 3 cut(s) 564, 1611, 2351
MaeII ACGT 9 cut(s) 348, 450, 618, 728, 739, 1038, 1044, 1506, 1926
MaeIII GTNAC 9 cut(s) 349, 740, 811, 1176, 1719, 1922, 1970, 2008, 2302
MalI GATC 9 cut(s) 915, 992, 1172, 1394, 1485, 1502, 1956, 2312, 2319
MbiI CCGCTC 2 cut(s) 438, 612
MboI GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
MfeI CAATTG 1 cut(s) 1831
MflI RGATCY 1 cut(s) 1954
MhlI GDGCHC 3 cut(s) 788, 1710, 1856
MluCI AATT 5 cut(s) 63, 96, 164, 467, 1831
MluI ACGCGT 2 cut(s) 795, 1602
Mly113I GGCGCC 1 cut(s) 2096
MlyI GAGTC 5 cut(s) 315, 457, 808, 1086, 1291
MmeI TCCRAC 2 cut(s) 270, 1488
Mph1103I ATGCAT 2 cut(s) 1406, 1876
MreI CGCCGGCG 2 cut(s) 854, 908
MroNI GCCGGC 6 cut(s) 229, 342, 833, 854, 908, 926
MroXI GAANNNNTTC 1 cut(s) 1874
MseI TTAA 4 cut(s) 225, 711, 1269, 1938
MslI CAYNNNNRTG 1 cut(s) 981
MspA1I CMGCKG 2 cut(s) 582, 1589
MunI CAATTG 1 cut(s) 1831
Mva1269I GAATGC 2 cut(s) 1874, 1876
MvaI CCWGG 7 cut(s) 515, 759, 790, 1156, 1349, 1653, 2100
NaeI GCCGGC 6 cut(s) 231, 344, 835, 856, 910, 928
NarI GGCGCC 1 cut(s) 2096
NdeI CATATG 1 cut(s) 2322
NdeII GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
NgoMIV GCCGGC 6 cut(s) 229, 342, 833, 854, 908, 926
NheI GCTAGC 1 cut(s) 563
NlaIV GGNNCC 7 cut(s) 496, 497, 553, 675, 1306, 2097, 2152
NmeAIII GCCGAG 2 cut(s) 294, 1518
NmuCI GTSAC 7 cut(s) 349, 740, 811, 1922, 1970, 2008, 2302
NruI TCGCGA 1 cut(s) 683
NsiI ATGCAT 2 cut(s) 1406, 1876
NspI RCATGY 2 cut(s) 1404, 2137
PaeI GCATGC 1 cut(s) 1404
PaeR7I CTCGAG 1 cut(s) 458
PagI TCATGA 1 cut(s) 993
PciI ACATGT 1 cut(s) 2133
PcsI WCGNNNNNNNCGW 4 cut(s) 456, 525, 654, 1284
PctI GAATGC 2 cut(s) 1874, 1876
PdiI GCCGGC 6 cut(s) 231, 344, 835, 856, 910, 928
PdmI GAANNNNTTC 1 cut(s) 1874
PfeI GAWTC 4 cut(s) 762, 951, 2109, 2285
PflFI GACNNNGTC 1 cut(s) 515
PflMI CCANNNNNTGG 2 cut(s) 430, 1354
PinAI ACCGGT 1 cut(s) 1904
PleI GAGTC 5 cut(s) 314, 457, 808, 1085, 1291
PluTI GGCGCC 1 cut(s) 2099
PmaCI CACGTG 2 cut(s) 349, 1507
PmlI CACGTG 2 cut(s) 349, 1507
PpsI GAGTC 5 cut(s) 314, 457, 808, 1085, 1291
Ppu21I YACGTR 3 cut(s) 349, 1039, 1507
PpuMI RGGWCCY 1 cut(s) 495
PscI ACATGT 1 cut(s) 2133
PsiI TTATAA 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 1856
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 7 cut(s) 513, 757, 788, 1154, 1347, 1651, 2098
PspCI CACGTG 2 cut(s) 349, 1507
PspEI GGTNACC 1 cut(s) 1719
PspFI CCCAGC 1 cut(s) 1312
PspGI CCWGG 7 cut(s) 513, 757, 788, 1154, 1347, 1651, 2098
PspN4I GGNNCC 7 cut(s) 496, 497, 553, 675, 1306, 2097, 2152
PspPPI RGGWCCY 1 cut(s) 495
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
PstNI CAGNNNCTG 2 cut(s) 943, 1186
PsuI RGATCY 1 cut(s) 1954
PsyI GACNNNGTC 1 cut(s) 515
RigI GGCCGGCC 1 cut(s) 837
RruI TCGCGA 1 cut(s) 683
RseI CAYNNNNRTG 1 cut(s) 981
Rsr2I CGGWCCG 1 cut(s) 273
RsrII CGGWCCG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 1856
SacII CCGCGG 1 cut(s) 1590
SalI GTCGAC 1 cut(s) 767
SaqAI TTAA 4 cut(s) 225, 711, 1269, 1938
Sau3AI GATC 9 cut(s) 913, 990, 1170, 1392, 1483, 1500, 1954, 2310, 2317
SchI GAGTC 5 cut(s) 315, 457, 808, 1086, 1291
SduI GDGCHC 3 cut(s) 788, 1710, 1856
SexAI ACCWGGT 1 cut(s) 513
SfaNI GCATC 4 cut(s) 1348, 1411, 1636, 2300
SfcI CTRYAG 2 cut(s) 19, 935
SfiI GGCCNNNNNGGCC 1 cut(s) 441
SfoI GGCGCC 1 cut(s) 2097
Sfr274I CTCGAG 1 cut(s) 458
Sfr303I CCGCGG 1 cut(s) 1590
SgrAI CRCCGGYG 2 cut(s) 854, 908
SgrBI CCGCGG 1 cut(s) 1590
SinI GGWCC 6 cut(s) 273, 495, 511, 673, 715, 1628
SlaI CTCGAG 1 cut(s) 458
SmaI CCCGGG 2 cut(s) 1739, 1766
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 3 cut(s) 458, 839, 2164
SmoI CTYRAG 3 cut(s) 458, 839, 2164
SpeI ACTAGT 1 cut(s) 2350
SphI GCATGC 1 cut(s) 1404
Sse9I AATT 5 cut(s) 63, 96, 164, 467, 1831
SspDI GGCGCC 1 cut(s) 2095
SspMI CTAG 3 cut(s) 564, 1611, 2351
SstI GAGCTC 1 cut(s) 1856
StyI CCWWGG 1 cut(s) 1837
TaaI ACNGT 3 cut(s) 277, 1433, 2074
TaiI ACGT 9 cut(s) 351, 453, 621, 731, 742, 1041, 1047, 1509, 1929
TasI AATT 5 cut(s) 63, 96, 164, 467, 1831
TatI WGTACW 6 cut(s) 1212, 1428, 1806, 1827, 2136, 2344
TauI GCSGC 9 cut(s) 323, 438, 447, 615, 635, 868, 1514, 1592, 1920
TfiI GAWTC 4 cut(s) 762, 951, 2109, 2285
Tru1I TTAA 4 cut(s) 225, 711, 1269, 1938
Tru9I TTAA 4 cut(s) 225, 711, 1269, 1938
TscAI CASTG 5 cut(s) 1289, 1423, 1759, 1863, 2077
TseFI GTSAC 7 cut(s) 349, 740, 811, 1922, 1970, 2008, 2302
TseI GCWGC 6 cut(s) 336, 339, 1552, 1844, 1946, 2027
Tsp45I GTSAC 7 cut(s) 349, 740, 811, 1922, 1970, 2008, 2302
TspDTI ATGAA 4 cut(s) 56, 89, 376, 1814
TspGWI ACGGA 4 cut(s) 686, 707, 892, 1178
TspMI CCCGGG 2 cut(s) 1737, 1764
TspRI CASTG 5 cut(s) 1289, 1423, 1759, 1863, 2077
Tth111I GACNNNGTC 1 cut(s) 515
Van91I CCANNNNNTGG 2 cut(s) 430, 1354
VneI GTGCAC 1 cut(s) 784
VpaK11BI GGWCC 6 cut(s) 273, 495, 511, 673, 715, 1628
XagI CCTNNNNNAGG 2 cut(s) 506, 1937
XapI RAATTY 1 cut(s) 164
XceI RCATGY 2 cut(s) 1404, 2137
XcmI CCANNNNNNNNNTGG 1 cut(s) 2089
XhoI CTCGAG 1 cut(s) 458
XmaI CCCGGG 2 cut(s) 1737, 1764
XmiI GTMKAC 1 cut(s) 768
XmnI GAANNNNTTC 1 cut(s) 1874
XspI CTAG 3 cut(s) 564, 1611, 2351
ZraI GACGTC 2 cut(s) 451, 1927
Zsp2I ATGCAT 2 cut(s) 1406, 1876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.