RLG00000015888

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009084
Physical Location & Seq
Forward (+)
2109518 .. 2112259
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015888

Sequence Viewer

Length: 2421 bp
ATGGTGTCCAATCTGTGTTACAGGTCTCCTCCTCTCACTCGTCTTATAAATACCTCTCGCCCAATTCATCTTCCCAAATCAAACCCTCATTCTCCAATTCATCTTCCCAAATCAAACCTTTCTTCTCCACTCCGAATTCGTAGATATTCCTGCAATCGAGCATTTCCGTTGTTGGCTTTCCAGAGAACCGAGAAGCCTTTTCATACCAGAAAGGACAGCCACAGCCCGATGACGATTAAGCCGGCGGTTCGCGTTTCCGACGGGAAACTGATAGTGAAGAACCGGACGGTTCTGACCGGATTACCGGACAATGTGGTCGCCACGTCCGGTTCGGCTTCCGGACCCGCCGAGGGAGTCTTCCTCGGCGCCGCGTTCGAGGAGGGCAGCAGCCGGCACGTGACTCCTCTCGGAACTTTACAAAACGGCGTGCGTTTCATGGCGTGCTTCCGGTTCAAGCTATGGTGGATGGCCCAGAAAATGGGCCGCTCCGGCCGCGACGTCCCTCTCGAGACTCAATTCCTATTGGTCGAGACCCAAGACGGGTCCCACCTCGAAGAGGACCAGATCGTGTACGCCGTGTTCCTCCCTCTTATCGAAGGCCCCTTCAGGGCTAGCTTACAGGGGAACAGCGGAGACGAGCTGGAGCTTTGTTTGGAGAGCGGTGACGTGGACACCAAAGCGGCGTCGTTTAGCCACGCGGTGTTCGTTCACGCCGGGACGGACCCGTTCGCGACGATAACGGAGGCGGTCAGGGCCGTTAAGGTTCACCTGAAGACGTTTCGTCAACGTCACGAGAAGAAGCTCCCTGGAATTGTTGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCAGGAAGTGACTCAGGAAGGAGTTGAGGCCGGCCTCAAGTCCCTCACCGCCGGAGGAGCGCCGCCGAAGTTTGTCATCATCGACGACGGGTGGCAGTCCGTAGCCGGCGATCAGGACAAAGCCGGCGAGCTACAGAGACTGACCGGAATCAAAGAAAACTCGAAGTTTCAACACAAGGATGATGGGATCATGACCATAGTGAACATAGCAAAAGAAAAATACGGGTTGAAATACGTGTACGTCTGGCACGCGATTACCGGCTACTGGGGAGGAGTCCGGCCCGGAACTGAATACGGGTCGGTTCTGAAGTACCCGGATTTGTCGAAAGGGGTTATGGAAAACGAACCGGCCTGGAAAACGGACATGATCCGGTTACAGGGTCTGGGCTTAGTGAACCCGAAAAGCGTGTACAAGTTTTACAACGAGCTACACAGCTACTTGGCCTCTGCGGGTGTAGATGGGGTTAAGGTGGACGCGCAGTGCGTGCTGGAGACTCTCGGAGCCGGGCTGGGCGGGCGGGTCGAGCTGACCCGGCAGTACCACCAGGCATTGGATGCTTCGGTGTCTCGGAACTTTGAAGATAATGGGATCATAGCATGCATGAGCCATAATACTGATGCGCTGTACTGTGAGAAACAGACGGCGGTGGTGAGAGCCTCCGACGATTTCTACCCGCGCGATCCGATGTCGCATACGATCCACGTGGCGGCGGTGGCGTATAATAGCGTTTTCCTCGGCGAGTTTATGCTGCCGGACTGGGATATGTTCCACTCGCTCCACCCGGCGGCTGAGTACCACGCGTCCGCTAGAGCCATTAGTGGTGGACCTGTTTATGTTAGTGATGCGCCTGGGAAGCATGACTTTGAGCTTTTGAAGAAGATGGTATTGCCGGACGGGTCGGTGCTCCGGGCGCGGTTACCCGGAAGGCCGACCCGGGACTGCTTGTTCACTGACCCGGCCCGGGATGGGGTGAGTTTGTTGAAGATATGGAACATGAATAAGTACACCGGTGTAGTGGGGGTGTACAATTGCCAAGGAGCAGCATGGAGTTCACTGGAGAGAAAGAATGCATTCCACGAGACGAAATCGGACGCCATAACCGGTTATGTTCGCGGCCGTGACGTCCACCTCATTAAGGAGGCAGCCACTGATCCCGATTGGAATGGTGACTGCGCCGTGTATTGCAATCGGACAGGGGAGCTTGTGACATTGCCTTACAATGCTGCCATGCCTATTTCCCTCAAAGTGCTTGAACATGAGATTTTCACTGTCAGCCCCATCAGGGTTTTGGCGCCCGGCGTGAGATTCGCTCCTTTGGGACTGGTTGACATGTACAATGCCGGTGGAGCCATTGAAGAGTTGAGATATGAGCCGCAGAGATTAGTCTCTATGGAGGTTAAAGGGTGTGGCAAATTTGGTGTGTATTCTTCAGAGAAGCCCAGGAGGTGTTGCGTTGGGACTAATGAGATTGATTTCAGCTATGATTCGGCATCTGGATTGGTGACTTTGAGCTTGGATCATATGCCTGAAGAGGGAAAAAGAGTACAGCCAGTCGAGGTTGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

807

Amino Acids

89.08

Weight (kDa)

6.71

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 83 - 788 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 2 cut(s) 501, 1977
AccB1I GGYRCC 2 cut(s) 365, 2143
AccB7I CCANNNNNTGG 2 cut(s) 478, 1402
AccBSI CCGCTC 2 cut(s) 486, 660
AccIII TCCGGA 1 cut(s) 338
AclWI GGATC 7 cut(s) 1046, 1213, 1448, 1526, 1543, 1997, 2376
AcoI YGGCCR 2 cut(s) 490, 1966
AcsI RAATTY 2 cut(s) 135, 2264
AcuI CTGAAG 5 cut(s) 589, 791, 1178, 2265, 2400
AcvI CACGTG 2 cut(s) 397, 1555
AcyI GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
AdeI CACNNNGTG 1 cut(s) 700
AflIII ACRYGT 4 cut(s) 843, 1086, 1650, 2181
AgeI ACCGGT 2 cut(s) 1859, 1952
AgsI TTSAA 8 cut(s) 454, 1022, 1081, 1430, 1726, 1834, 2105, 2207
AhdI GACNNNNNGTC 1 cut(s) 780
AjiI CACGTC 2 cut(s) 324, 667
AjnI CCWGG 7 cut(s) 805, 836, 852, 1202, 1395, 1699, 2291
AloI GAACNNNNNNTCC 2 cut(s) 1781, 1813
Alw21I GWGCWC 2 cut(s) 836, 1758
Alw26I GTCTC 9 cut(s) 30, 503, 524, 627, 982, 1337, 1422, 1925, 2242
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 7 cut(s) 1046, 1213, 1448, 1526, 1543, 1997, 2376
AlwNI CAGNNNCTG 3 cut(s) 991, 1234, 2000
Ama87I CYCGRG 3 cut(s) 506, 1785, 1812
Aor13HI TCCGGA 1 cut(s) 338
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 6 cut(s) 384, 387, 1600, 1892, 1994, 2075
ApoI RAATTY 2 cut(s) 135, 2264
AsiGI ACCGGT 2 cut(s) 1859, 1952
Asp700I GAANNNNTTC 1 cut(s) 1922
AspLEI GCGC 9 cut(s) 368, 913, 1330, 1474, 1530, 1699, 1765, 2027, 2146
AsuHPI GGTGA 7 cut(s) 674, 758, 889, 1513, 1834, 2030, 2365
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 3 cut(s) 506, 1785, 1812
AvaII GGWCC 5 cut(s) 341, 543, 559, 721, 1676
BaeGI GKGCMC 1 cut(s) 836
BanI GGYRCC 2 cut(s) 365, 2143
BauI CACGAG 2 cut(s) 791, 1928
BbrPI CACGTG 2 cut(s) 397, 1555
BbsI GAAGAC 2 cut(s) 349, 779
Bbv12I GWGCWC 2 cut(s) 836, 1758
BbvI GCAGC 6 cut(s) 396, 399, 1587, 1904, 2006, 2062
BccI CCATC 6 cut(s) 460, 1028, 1304, 1726, 1811, 2138
BceAI ACGGC 6 cut(s) 439, 560, 740, 1509, 1953, 2012
BciT130I CCWGG 7 cut(s) 807, 838, 854, 1204, 1397, 1701, 2293
BcoDI GTCTC 9 cut(s) 30, 503, 524, 627, 982, 1337, 1422, 1925, 2242
BfaI CTAG 2 cut(s) 612, 1659
BfmI CTRYAG 1 cut(s) 983
BfoI RGCGCY 3 cut(s) 369, 914, 2147
BglI GCCNNNNNGGC 1 cut(s) 489
Bme18I GGWCC 5 cut(s) 341, 543, 559, 721, 1676
BmeRI GACNNNNNGTC 1 cut(s) 780
BmeT110I CYCGRG 3 cut(s) 506, 1785, 1812
BmgBI CACGTC 2 cut(s) 324, 667
BmiI GGNNCC 9 cut(s) 343, 367, 544, 545, 601, 723, 1354, 2145, 2200
BmrI ACTGGG 2 cut(s) 1126, 1618
BmsI GCATC 4 cut(s) 1396, 1459, 1684, 2351
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 1126, 1618
BpiI GAAGAC 2 cut(s) 349, 779
BplI GAGNNNNNCTC 4 cut(s) 345, 377, 2146, 2178
BpmI CTGGAG 3 cut(s) 662, 1361, 1928
BpuEI CTTGAG 1 cut(s) 872
BsaAI YACGTR 3 cut(s) 397, 1087, 1555
BsaHI GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
BsaI GGTCTC 2 cut(s) 30, 524
Bse1I ACTGG 5 cut(s) 1121, 1613, 1911, 2178, 2402
Bse3DI GCAATG 1 cut(s) 2060
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 7 cut(s) 807, 838, 854, 1204, 1397, 1701, 2293
BseGI GGATG 4 cut(s) 471, 1036, 1411, 1822
BseMI GCAATG 1 cut(s) 2060
BseMII CTCAG 2 cut(s) 878, 1632
BseNI ACTGG 5 cut(s) 1121, 1613, 1911, 2178, 2402
BseRI GAGGAG 6 cut(s) 18, 21, 392, 393, 921, 1137
BseSI GKGCMC 1 cut(s) 836
BseX3I CGGCCG 2 cut(s) 490, 1966
BseXI GCAGC 6 cut(s) 396, 399, 1587, 1904, 2006, 2062
BseYI CCCAGC 1 cut(s) 1360
Bsh1285I CGRYCG 2 cut(s) 493, 1969
BshNI GGYRCC 2 cut(s) 365, 2143
BshTI ACCGGT 2 cut(s) 1859, 1952
BsiEI CGRYCG 2 cut(s) 493, 1969
BsiHKAI GWGCWC 2 cut(s) 836, 1758
BsiHKCI CYCGRG 3 cut(s) 506, 1785, 1812
BslFI GGGAC 8 cut(s) 485, 529, 730, 854, 877, 1802, 2184, 2323
BsmAI GTCTC 9 cut(s) 30, 503, 524, 627, 982, 1337, 1422, 1925, 2242
BsmBI CGTCTC 2 cut(s) 627, 1925
BsmFI GGGAC 8 cut(s) 485, 529, 730, 854, 877, 1802, 2184, 2323
BsmI GAATGC 2 cut(s) 1922, 1924
Bso31I GGTCTC 2 cut(s) 30, 524
BsoBI CYCGRG 3 cut(s) 506, 1785, 1812
Bsp1286I GDGCHC 2 cut(s) 836, 1758
Bsp13I TCCGGA 1 cut(s) 338
Bsp1407I TGTACA 3 cut(s) 1260, 1875, 2184
Bsp143I GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
Bsp68I TCGCGA 1 cut(s) 731
BspCNI CTCAG 2 cut(s) 877, 1633
BspEI TCCGGA 1 cut(s) 338
BspHI TCATGA 1 cut(s) 1041
BspLI GGNNCC 9 cut(s) 343, 367, 544, 545, 601, 723, 1354, 2145, 2200
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 7 cut(s) 1046, 1213, 1448, 1526, 1543, 1997, 2376
BspT107I GGYRCC 2 cut(s) 365, 2143
BspTNI GGTCTC 2 cut(s) 30, 524
BsrBI CCGCTC 2 cut(s) 486, 660
BsrDI GCAATG 1 cut(s) 2060
BsrGI TGTACA 3 cut(s) 1260, 1875, 2184
BsrI ACTGG 5 cut(s) 1121, 1613, 1911, 2178, 2402
BssMI GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
BssNI GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
BssSI CACGAG 2 cut(s) 791, 1928
BssT1I CCWWGG 1 cut(s) 1885
Bst2BI CACGAG 2 cut(s) 791, 1928
Bst2UI CCWGG 7 cut(s) 807, 838, 854, 1204, 1397, 1701, 2293
Bst4CI ACNGT 3 cut(s) 289, 1481, 2122
Bst6I CTCTTC 3 cut(s) 549, 2202, 2376
BstACI GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
BstAPI GCANNNNNTGC 2 cut(s) 1336, 1406
BstAUI TGTACA 3 cut(s) 1260, 1875, 2184
BstBAI YACGTR 3 cut(s) 397, 1087, 1555
BstDEI CTNAG 3 cut(s) 864, 1240, 1641
BstEII GGTNACC 1 cut(s) 1767
BstENI CCTNNNNNAGG 2 cut(s) 554, 1985
BstF5I GGATG 4 cut(s) 471, 1036, 1411, 1822
BstH2I RGCGCY 3 cut(s) 369, 914, 2147
BstHHI GCGC 9 cut(s) 368, 913, 1330, 1474, 1530, 1699, 1765, 2027, 2146
BstKTI GATC 9 cut(s) 567, 964, 1041, 1221, 1443, 1534, 1551, 2005, 2371
BstMAI GTCTC 9 cut(s) 30, 503, 524, 627, 982, 1337, 1422, 1925, 2242
BstMBI GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
BstMCI CGRYCG 2 cut(s) 493, 1969
BstNI CCWGG 7 cut(s) 807, 838, 854, 1204, 1397, 1701, 2293
BstNSI RCATGY 2 cut(s) 1452, 2185
BstPI GGTNACC 1 cut(s) 1767
BstSFI CTRYAG 1 cut(s) 983
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 6 cut(s) 396, 399, 1587, 1904, 2006, 2062
BstV2I GAAGAC 2 cut(s) 349, 779
BstZI CGGCCG 2 cut(s) 490, 1966
BtrI CACGTC 2 cut(s) 324, 667
BtsCI GGATG 4 cut(s) 471, 1036, 1411, 1822
BtsI GCAGTG 1 cut(s) 1337
BtsIMutI CAGTG 5 cut(s) 1337, 1800, 1904, 1998, 2118
BtuMI TCGCGA 1 cut(s) 731
CaiI CAGNNNCTG 3 cut(s) 991, 1234, 2000
CciI TCATGA 1 cut(s) 1041
CfoI GCGC 9 cut(s) 368, 913, 1330, 1474, 1530, 1699, 1765, 2027, 2146
Cfr9I CCCGGG 2 cut(s) 1785, 1812
CseI GACGC 5 cut(s) 672, 851, 1334, 1641, 1952
CspAI ACCGGT 2 cut(s) 1859, 1952
CspCI CAANNNNNGTGG 2 cut(s) 2176, 2211
DdeI CTNAG 3 cut(s) 864, 1240, 1641
DinI GGCGCC 2 cut(s) 367, 2145
DpnI GATC 9 cut(s) 566, 963, 1040, 1220, 1442, 1533, 1550, 2004, 2370
DpnII GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
DraIII CACNNNGTG 1 cut(s) 700
DrdI GACNNNNNNGTC 1 cut(s) 314
DriI GACNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 2 cut(s) 490, 1966
EagI CGGCCG 2 cut(s) 490, 1966
Eam1104I CTCTTC 3 cut(s) 549, 2202, 2376
Eam1105I GACNNNNNGTC 1 cut(s) 780
EarI CTCTTC 3 cut(s) 549, 2202, 2376
EclXI CGGCCG 2 cut(s) 490, 1966
Eco130I CCWWGG 1 cut(s) 1885
Eco31I GGTCTC 2 cut(s) 30, 524
Eco47I GGWCC 5 cut(s) 341, 543, 559, 721, 1676
Eco52I CGGCCG 2 cut(s) 490, 1966
Eco57I CTGAAG 5 cut(s) 589, 791, 1178, 2265, 2400
Eco72I CACGTG 2 cut(s) 397, 1555
Eco88I CYCGRG 3 cut(s) 506, 1785, 1812
Eco91I GGTNACC 1 cut(s) 1767
EcoNI CCTNNNNNAGG 2 cut(s) 554, 1985
EcoO109I RGGNCCY 2 cut(s) 543, 599
EcoO65I GGTNACC 1 cut(s) 1767
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 7 cut(s) 805, 836, 852, 1202, 1395, 1699, 2291
EcoT14I CCWWGG 1 cut(s) 1885
EcoT22I ATGCAT 2 cut(s) 1454, 1924
EgeI GGCGCC 2 cut(s) 367, 2145
EheI GGCGCC 2 cut(s) 367, 2145
ErhI CCWWGG 1 cut(s) 1885
Esp3I CGTCTC 2 cut(s) 627, 1925
FalI AAGNNNNNCTT 2 cut(s) 1697, 1729
FaqI GGGAC 8 cut(s) 485, 529, 730, 854, 877, 1802, 2184, 2323
FauI CCCGC 5 cut(s) 352, 1294, 1358, 1362, 1533
FauNDI CATATG 1 cut(s) 2373
FokI GGATG 4 cut(s) 478, 1043, 1418, 1829
FseI GGCCGGCC 1 cut(s) 885
FspBI CTAG 2 cut(s) 612, 1659
GlaI GCGC 9 cut(s) 367, 912, 1329, 1473, 1529, 1698, 1764, 2026, 2145
GsaI CCCAGC 1 cut(s) 1364
GsuI CTGGAG 3 cut(s) 662, 1361, 1928
HaeII RGCGCY 3 cut(s) 369, 914, 2147
HgaI GACGC 5 cut(s) 672, 851, 1334, 1641, 1952
HhaI GCGC 9 cut(s) 368, 913, 1330, 1474, 1530, 1699, 1765, 2027, 2146
Hin1I GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
Hin6I GCGC 9 cut(s) 366, 911, 1328, 1472, 1528, 1697, 1763, 2025, 2144
HinP1I GCGC 9 cut(s) 366, 911, 1328, 1472, 1528, 1697, 1763, 2025, 2144
HincII GTYRAC 3 cut(s) 785, 817, 2179
HindII GTYRAC 3 cut(s) 785, 817, 2179
HinfI GANTC 9 cut(s) 354, 400, 511, 862, 999, 1125, 1345, 2157, 2336
HphI GGTGA 7 cut(s) 674, 758, 889, 1513, 1834, 2030, 2365
Hpy99I CGWCG 7 cut(s) 263, 500, 688, 736, 938, 941, 1517
HpyAV CCTTC 4 cut(s) 590, 613, 863, 1770
HpyCH4III ACNGT 3 cut(s) 289, 1481, 2122
HpyCH4V TGCA 5 cut(s) 153, 834, 1452, 1922, 2037
HpyF3I CTNAG 3 cut(s) 864, 1240, 1641
Hsp92I GRCGYC 6 cut(s) 366, 498, 683, 1944, 1974, 2144
HspAI GCGC 9 cut(s) 366, 911, 1328, 1472, 1528, 1697, 1763, 2025, 2144
KasI GGCGCC 2 cut(s) 365, 2143
KflI GGGWCCC 1 cut(s) 543
Kpn2I TCCGGA 1 cut(s) 338
KroI GCCGGC 5 cut(s) 241, 390, 881, 956, 974
KroNI GCCGGC 5 cut(s) 243, 392, 883, 958, 976
Kzo9I GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
Lsp1109I GCAGC 6 cut(s) 396, 399, 1587, 1904, 2006, 2062
LweI GCATC 4 cut(s) 1396, 1459, 1684, 2351
MaeI CTAG 2 cut(s) 612, 1659
MalI GATC 9 cut(s) 566, 963, 1040, 1220, 1442, 1533, 1550, 2004, 2370
MbiI CCGCTC 2 cut(s) 486, 660
MboI GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
MfeI CAATTG 1 cut(s) 1879
MhlI GDGCHC 2 cut(s) 836, 1758
MluCI AATT 7 cut(s) 63, 96, 135, 515, 810, 1879, 2264
MluI ACGCGT 2 cut(s) 843, 1650
Mly113I GGCGCC 2 cut(s) 366, 2144
MlyI GAGTC 6 cut(s) 363, 394, 505, 856, 1134, 1339
MmeI TCCRAC 2 cut(s) 282, 1536
Mph1103I ATGCAT 2 cut(s) 1454, 1924
MroI TCCGGA 1 cut(s) 338
MroNI GCCGGC 5 cut(s) 241, 390, 881, 956, 974
MroXI GAANNNNTTC 1 cut(s) 1922
MseI TTAA 5 cut(s) 237, 759, 1317, 1986, 2250
MslI CAYNNNNRTG 1 cut(s) 1029
MspA1I CMGCKG 1 cut(s) 630
MunI CAATTG 1 cut(s) 1879
Mva1269I GAATGC 2 cut(s) 1922, 1924
MvaI CCWGG 7 cut(s) 807, 838, 854, 1204, 1397, 1701, 2293
NaeI GCCGGC 5 cut(s) 243, 392, 883, 958, 976
NarI GGCGCC 2 cut(s) 366, 2144
NdeI CATATG 1 cut(s) 2373
NdeII GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
NgoMIV GCCGGC 5 cut(s) 241, 390, 881, 956, 974
NheI GCTAGC 1 cut(s) 611
NlaIV GGNNCC 9 cut(s) 343, 367, 544, 545, 601, 723, 1354, 2145, 2200
NmeAIII GCCGAG 3 cut(s) 342, 373, 1566
NmuCI GTSAC 8 cut(s) 397, 662, 788, 859, 1970, 2018, 2056, 2353
NruI TCGCGA 1 cut(s) 731
NsiI ATGCAT 2 cut(s) 1454, 1924
NspI RCATGY 2 cut(s) 1452, 2185
PaeI GCATGC 1 cut(s) 1452
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 1041
PciI ACATGT 1 cut(s) 2181
PcsI WCGNNNNNNNCGW 4 cut(s) 504, 573, 702, 1332
PctI GAATGC 2 cut(s) 1922, 1924
PdiI GCCGGC 5 cut(s) 243, 392, 883, 958, 976
PdmI GAANNNNTTC 1 cut(s) 1922
PfeI GAWTC 3 cut(s) 999, 2157, 2336
PflMI CCANNNNNTGG 2 cut(s) 478, 1402
PinAI ACCGGT 2 cut(s) 1859, 1952
PleI GAGTC 6 cut(s) 362, 394, 505, 856, 1133, 1339
PluTI GGCGCC 2 cut(s) 369, 2147
PmaCI CACGTG 2 cut(s) 397, 1555
PmlI CACGTG 2 cut(s) 397, 1555
PpsI GAGTC 6 cut(s) 362, 394, 505, 856, 1133, 1339
Ppu21I YACGTR 3 cut(s) 397, 1087, 1555
PpuMI RGGWCCY 1 cut(s) 543
PscI ACATGT 1 cut(s) 2181
PsiI TTATAA 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 7 cut(s) 805, 836, 852, 1202, 1395, 1699, 2291
PspCI CACGTG 2 cut(s) 397, 1555
PspEI GGTNACC 1 cut(s) 1767
PspFI CCCAGC 1 cut(s) 1360
PspGI CCWGG 7 cut(s) 805, 836, 852, 1202, 1395, 1699, 2291
PspN4I GGNNCC 9 cut(s) 343, 367, 544, 545, 601, 723, 1354, 2145, 2200
PspPPI RGGWCCY 1 cut(s) 543
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PstNI CAGNNNCTG 3 cut(s) 991, 1234, 2000
RigI GGCCGGCC 1 cut(s) 885
RruI TCGCGA 1 cut(s) 731
RseI CAYNNNNRTG 1 cut(s) 1029
SaqAI TTAA 5 cut(s) 237, 759, 1317, 1986, 2250
Sau3AI GATC 9 cut(s) 564, 961, 1038, 1218, 1440, 1531, 1548, 2002, 2368
SchI GAGTC 6 cut(s) 363, 394, 505, 856, 1134, 1339
SduI GDGCHC 2 cut(s) 836, 1758
SfaNI GCATC 4 cut(s) 1396, 1459, 1684, 2351
SfcI CTRYAG 1 cut(s) 983
SfiI GGCCNNNNNGGCC 1 cut(s) 489
SfoI GGCGCC 2 cut(s) 367, 2145
Sfr274I CTCGAG 1 cut(s) 506
SgrAI CRCCGGYG 1 cut(s) 1859
SinI GGWCC 5 cut(s) 341, 543, 559, 721, 1676
SlaI CTCGAG 1 cut(s) 506
SmaI CCCGGG 2 cut(s) 1787, 1814
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 2 cut(s) 506, 887
SmoI CTYRAG 2 cut(s) 506, 887
SphI GCATGC 1 cut(s) 1452
Sse9I AATT 7 cut(s) 63, 96, 135, 515, 810, 1879, 2264
SspDI GGCGCC 2 cut(s) 365, 2143
SspMI CTAG 2 cut(s) 612, 1659
StyI CCWWGG 1 cut(s) 1885
TaaI ACNGT 3 cut(s) 289, 1481, 2122
TasI AATT 7 cut(s) 63, 96, 135, 515, 810, 1879, 2264
TatI WGTACW 6 cut(s) 1260, 1476, 1854, 1875, 2184, 2395
TauI GCSGC 9 cut(s) 371, 486, 495, 683, 916, 1562, 1640, 1968, 2227
TfiI GAWTC 3 cut(s) 999, 2157, 2336
Tru1I TTAA 5 cut(s) 237, 759, 1317, 1986, 2250
Tru9I TTAA 5 cut(s) 237, 759, 1317, 1986, 2250
TscAI CASTG 5 cut(s) 1337, 1807, 1911, 2005, 2125
TseFI GTSAC 8 cut(s) 397, 662, 788, 859, 1970, 2018, 2056, 2353
TseI GCWGC 6 cut(s) 384, 387, 1600, 1892, 1994, 2075
Tsp45I GTSAC 8 cut(s) 397, 662, 788, 859, 1970, 2018, 2056, 2353
TspDTI ATGAA 5 cut(s) 56, 89, 191, 424, 1862
TspGWI ACGGA 5 cut(s) 156, 734, 755, 940, 1226
TspMI CCCGGG 2 cut(s) 1785, 1812
TspRI CASTG 5 cut(s) 1337, 1807, 1911, 2005, 2125
Van91I CCANNNNNTGG 2 cut(s) 478, 1402
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 5 cut(s) 341, 543, 559, 721, 1676
XagI CCTNNNNNAGG 2 cut(s) 554, 1985
XapI RAATTY 2 cut(s) 135, 2264
XceI RCATGY 2 cut(s) 1452, 2185
XcmI CCANNNNNNNNNTGG 1 cut(s) 2137
XhoI CTCGAG 1 cut(s) 506
XmaI CCCGGG 2 cut(s) 1785, 1812
XmnI GAANNNNTTC 1 cut(s) 1922
XspI CTAG 2 cut(s) 612, 1659
ZraI GACGTC 2 cut(s) 499, 1975
Zsp2I ATGCAT 2 cut(s) 1454, 1924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.