MD02G1025500.v1.1

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009084
Physical Location & Seq
Forward (+)
1927553 .. 1930057
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1025500.v1.1.491

Sequence Viewer

Length: 2136 bp
ATGGTGTCAAATTCGTGTAGCAGTTCTCTATCTCGTATTATAAATACCAGCCCCATTATTCATTTACCCAAATCAAATTATCCCAAGCATAATTTACCCAAATCAAATTATCCCAAGCATTCTCCTACTACAAGATTCAATCTTCCGAACCCAAACAACTTGTACTTTCTCAGGCGCTCTGTAATTTCTCGTCCTGCTACTACTTCTGATTTTCTCGGAATCGGGCGTCTTCGTTTTTGGCTTTTCAGAGAAGAAAGAGAGATGACGATCAAACCGGCGGTTCGAATCTCGGAACGGAAGCTGATCGTGAAGGACCGGACGATTCTGACGGGATTGCCGGACAATGTAGTGGCCACGTCCGGTTCGTCTTCCGGTCCGGTCGACGGGGTCTTCCTCGGCGCGAATTTCTCGGAGGAGAAAAGCCGGCACGTGGTTTCGTTAGGGACGCTTACCGGAGTCCGGTTCATGGCGTGCTTCCGGTTCAAGCTGTGGTGGATGGCCCAGAAGATGGGGGATCAGGGAAGGGACATACCTCTTGAGACTCAATTCCTATTGCTCGAGACCAAGCACGGGTCCCACCTGGAATCGGACGGCGGAGACGAAGAGAACCAGATCGTCTACACCGTGTTCCTCCCTCTCATCGAAGGCTCGTTCCGCGGTTGCCTGCAGGGCAACGCGAGCGACGAGCTCGAGCTCTGTTTGGAGAGCGGCGACGCCGACACCAAAGCGTCGTCGTTTTCCCACGCACTGCACGTCCATGCCGGGACGGACCCGTTCGGTACGATAACGGAAGCGATCAGGTCCGTCAAAGTTCACCTTCAGACGTTTCGTCAACGTCACGAGAAGAAACTCCCCGGATTCGTTGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCGGGACGTGACTCAGGAGGACGTGGAGGCCGGGCTCGAGTCTTTAGCCGCCGGTGGAACACCTCCGAAGTTCGTTATCATCGACGATGGGTGGCAGTCCGTCGTCGGCGGCGACGACGGCGTTGAAAAGCAGGAGCCGCTAAGGCTGACTGGGATCAAAGAAAATTCAAAGTTTCAGAAGAAGGACGATCCGACAGTGGGGATTAAGAACATCGTCAGCATCGCGAAACAAAAGCACGGGTTGAAGTATGTGTACGTGTGGCACGCGATTACTGGCTACTGGGGCGGGGTCAGACCCGGAATCAAAGAGATGGAGGAATTCGGATCCTTGATGAAGTACCCGAACGTTTCCAGCGGTGTTGTGGCCAACGAGCCGACGTGGAAGACGGACGCGATGGCGGTGCGAGGGTTGGGGCTGGTGGATCCGAAGGGCGTGTACAAATTCTACAACGAATTGCACAGCTACTTGAGCTCTGCGGGTATTGACGGCGTTAAGGTGGACGTGCAGTGCATACTGGAAACTCTCGGAGCCGGGCTAGGCGGTCGGGTCGAGTTGACCCAGAAGTACCACCAGGCGCTGGACGCCTCAGTGGCGAGGAATTTCCCGGACAACGGCTGCATCGCGTGCATGAGCCACAATACTGACGCGCTCTACTGCTCCAAACAGACCGCCGTCGTGAGGGCGTCCGACGATTTCTACCCCCGCGACCCGGTGTCGCACACGATCCACATTGCGGCGGTTGCTTACAACAGCGTGTTTCTCGGCGAGTTTATGCTGCCAGACTGGGACATGTTCCAGTCGCTCCATCCGGCGGCTGAGTACCACGCTTCCGCCAGGGCCGTCAGTGGTGGGCCCATCTATGTCAGTGACGCGCCTGGGAAGCACAATTTCGAGCTGCTGAGGAAGCTGGTGTTGCCGGACGGGTCAGTGCTTCGGGCCCGATTGCCTGGACGCCCGACGAAAGACTGCCTGTTCTCTGACCCGGCCCGCGACGGCGTGAGCTTGCTGAAGATATGGAACATGAACAAGTACACCGGAGTCCTCGGCGTCTACAACTGCCAAGGCGCAGCGTGGAGCACCGCGGAACGGAAGAACACGTTCCACGAAACCAAATCCGAGGCCATCACGGGTCTGATTCGCGGCCGCGACGTCCACCTGATTGCGGAGGCCGCCGTGGACAACGACTGGAAAGGCGACTGCGCCGTGTACAGTCACCGGACGGGAGAGAGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

712

Amino Acids

78.74

Weight (kDa)

6.93

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 94 - 709 9.1e-294 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AatII GACGTC 1 cut(s) 2052
AccB7I CCANNNNNTGG 2 cut(s) 508, 1477
AccBSI CCGCTC 1 cut(s) 708
AccI GTMKAC 3 cut(s) 381, 618, 1950
AclI AACGTT 1 cut(s) 1245
AclWI GGATC 8 cut(s) 522, 1061, 1082, 1218, 1231, 1316, 1329, 1618
AcoI YGGCCR 3 cut(s) 351, 1263, 2041
AcsI RAATTY 6 cut(s) 10, 403, 1063, 1217, 1340, 1498
AcuI CTGAAG 2 cut(s) 803, 1928
AcvI CACGTG 1 cut(s) 430
AcyI GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
AfaI GTAC 9 cut(s) 164, 781, 1154, 1238, 1337, 1466, 1721, 1931, 2108
AflIII ACRYGT 4 cut(s) 891, 1155, 1689, 1995
AgsI TTSAA 5 cut(s) 139, 484, 1025, 1068, 1144
AhdI GACNNNNNGTC 2 cut(s) 828, 1612
AjiI CACGTC 6 cut(s) 357, 754, 907, 922, 1278, 1402
AjnI CCWGG 6 cut(s) 579, 884, 1470, 1733, 1774, 1846
Alw21I GWGCWC 5 cut(s) 690, 696, 884, 1373, 1979
Alw26I GTCTC 3 cut(s) 533, 554, 591
Alw44I GTGCAC 1 cut(s) 880
AlwI GGATC 8 cut(s) 522, 1061, 1082, 1218, 1231, 1316, 1329, 1618
AlwNI CAGNNNCTG 1 cut(s) 1477
Ama87I CYCGRG 3 cut(s) 557, 689, 935
ApaI GGGCCC 2 cut(s) 1755, 1840
ApaLI GTGCAC 1 cut(s) 880
ApeKI GCWGC 4 cut(s) 1515, 1675, 1795, 1967
ApoI RAATTY 6 cut(s) 10, 403, 1063, 1217, 1340, 1498
Asp700I GAANNNNTTC 1 cut(s) 1997
AspLEI GCGC 7 cut(s) 177, 401, 1477, 1549, 1774, 1967, 2102
AsuC2I CCSGG 9 cut(s) 763, 855, 902, 931, 1197, 1432, 1505, 1610, 1883
AsuHPI GGTGA 2 cut(s) 806, 2105
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 3 cut(s) 557, 689, 935
AvaII GGWCC 5 cut(s) 313, 374, 573, 769, 801
BaeGI GKGCMC 3 cut(s) 884, 1755, 1840
BalI TGGCCA 2 cut(s) 353, 1265
BamHI GGATCC 2 cut(s) 1223, 1321
BanII GRGCYC 6 cut(s) 690, 696, 936, 1373, 1755, 1840
BarI GAAGNNNNNNTAC 4 cut(s) 1136, 1168, 1319, 1351
BauI CACGAG 1 cut(s) 839
BbrPI CACGTG 1 cut(s) 430
BbsI GAAGAC 4 cut(s) 221, 360, 382, 1289
Bbv12I GWGCWC 5 cut(s) 690, 696, 884, 1373, 1979
BbvCI CCTCAGC 1 cut(s) 1799
BbvI GCAGC 4 cut(s) 1502, 1662, 1782, 1979
BccI CCATC 8 cut(s) 490, 502, 980, 1204, 1288, 1713, 1763, 2030
BceAI ACGGC 9 cut(s) 607, 1033, 1402, 1528, 1556, 1724, 1909, 2057, 2087
BcgI CGANNNNNNTGC 2 cut(s) 1282, 1316
BciT130I CCWGG 6 cut(s) 581, 886, 1472, 1735, 1776, 1848
BcnI CCSGG 9 cut(s) 763, 855, 902, 931, 1197, 1432, 1505, 1610, 1883
BcoDI GTCTC 3 cut(s) 533, 554, 591
BfaI CTAG 1 cut(s) 1436
BfmI CTRYAG 1 cut(s) 665
BfoI RGCGCY 2 cut(s) 178, 1478
BglI GCCNNNNNGGC 3 cut(s) 669, 1042, 1490
Bme18I GGWCC 5 cut(s) 313, 374, 573, 769, 801
BmeRI GACNNNNNGTC 2 cut(s) 828, 1612
BmeT110I CYCGRG 3 cut(s) 557, 689, 935
BmgBI CACGTC 6 cut(s) 357, 754, 907, 922, 1278, 1402
BmiI GGNNCC 9 cut(s) 574, 575, 771, 1035, 1225, 1323, 1429, 1753, 1838
BmrI ACTGGG 3 cut(s) 1059, 1189, 1693
BmsI GCATC 2 cut(s) 1128, 1527
BmuI ACTGGG 3 cut(s) 1059, 1189, 1693
BoxI GACNNNNGTC 1 cut(s) 1571
BpiI GAAGAC 4 cut(s) 221, 360, 382, 1289
Bpu10I CCTNAGC 2 cut(s) 1040, 1799
Bpu14I TTCGAA 1 cut(s) 283
BpuEI CTTGAG 2 cut(s) 557, 1387
BpuMI CCSGG 9 cut(s) 763, 855, 902, 931, 1197, 1432, 1505, 1610, 1883
BsaAI YACGTR 2 cut(s) 430, 1156
BsaBI GATNNNNATC 1 cut(s) 266
BsaHI GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
BsaI GGTCTC 1 cut(s) 554
BsaWI WCCGGW 9 cut(s) 315, 359, 371, 376, 452, 459, 477, 1934, 2115
Bse118I RCCGGY 3 cut(s) 274, 423, 950
Bse1I ACTGG 7 cut(s) 1054, 1177, 1184, 1419, 1688, 1696, 2090
Bse3DI GCAATG 1 cut(s) 1629
Bse8I GATNNNNATC 1 cut(s) 266
BseBI CCWGG 6 cut(s) 581, 886, 1472, 1735, 1776, 1848
BseGI GGATG 2 cut(s) 501, 1705
BseJI GATNNNNATC 1 cut(s) 266
BseMI GCAATG 1 cut(s) 1629
BseMII CTCAG 5 cut(s) 184, 926, 1500, 1707, 1790
BseNI ACTGG 7 cut(s) 1054, 1177, 1184, 1419, 1688, 1696, 2090
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 3 cut(s) 884, 1755, 1840
BseX3I CGGCCG 1 cut(s) 2041
BseXI GCAGC 4 cut(s) 1502, 1662, 1782, 1979
BsgI GTGCAG 2 cut(s) 734, 1424
Bsh1285I CGRYCG 3 cut(s) 381, 1444, 2044
BsiEI CGRYCG 3 cut(s) 381, 1444, 2044
BsiHKAI GWGCWC 5 cut(s) 690, 696, 884, 1373, 1979
BsiHKCI CYCGRG 3 cut(s) 557, 689, 935
BslFI GGGAC 7 cut(s) 457, 539, 559, 778, 902, 917, 1700
BsmAI GTCTC 3 cut(s) 533, 554, 591
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 7 cut(s) 457, 539, 559, 778, 902, 917, 1700
BsmI GAATGC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 3 cut(s) 557, 689, 935
Bsp119I TTCGAA 1 cut(s) 283
Bsp120I GGGCCC 2 cut(s) 1751, 1836
Bsp1286I GDGCHC 8 cut(s) 690, 696, 884, 936, 1373, 1755, 1840, 1979
Bsp1407I TGTACA 2 cut(s) 1335, 2106
Bsp68I TCGCGA 1 cut(s) 1124
BspCNI CTCAG 5 cut(s) 183, 925, 1499, 1708, 1791
BspLI GGNNCC 9 cut(s) 574, 575, 771, 1035, 1225, 1323, 1429, 1753, 1838
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 8 cut(s) 522, 1061, 1082, 1218, 1231, 1316, 1329, 1618
BspT104I TTCGAA 1 cut(s) 283
BspTNI GGTCTC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 708
BsrDI GCAATG 1 cut(s) 1629
BsrFI RCCGGY 3 cut(s) 274, 423, 950
BsrGI TGTACA 2 cut(s) 1335, 2106
BsrI ACTGG 7 cut(s) 1054, 1177, 1184, 1419, 1688, 1696, 2090
BssAI RCCGGY 3 cut(s) 274, 423, 950
BssNI GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
BssSI CACGAG 1 cut(s) 839
BssT1I CCWWGG 1 cut(s) 1960
Bst2BI CACGAG 1 cut(s) 839
Bst2UI CCWGG 6 cut(s) 581, 886, 1472, 1735, 1776, 1848
Bst4CI ACNGT 3 cut(s) 625, 1096, 2111
Bst6I CTCTTC 1 cut(s) 597
BstACI GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
BstAPI GCANNNNNTGC 1 cut(s) 1524
BstAUI TGTACA 2 cut(s) 1335, 2106
BstBAI YACGTR 2 cut(s) 430, 1156
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 8 cut(s) 425, 472, 665, 679, 1164, 1525, 1888, 1904
BstDEI CTNAG 6 cut(s) 170, 912, 1040, 1486, 1716, 1799
BstDSI CCRYGG 3 cut(s) 655, 1980, 2073
BstF5I GGATG 2 cut(s) 501, 1705
BstH2I RGCGCY 2 cut(s) 178, 1478
BstHHI GCGC 7 cut(s) 177, 401, 1477, 1549, 1774, 1967, 2102
BstMAI GTCTC 3 cut(s) 533, 554, 591
BstMCI CGRYCG 3 cut(s) 381, 1444, 2044
BstNI CCWGG 6 cut(s) 581, 886, 1472, 1735, 1776, 1848
BstNSI RCATGY 1 cut(s) 1693
BstPAI GACNNNNGTC 1 cut(s) 1571
BstSFI CTRYAG 1 cut(s) 665
BstSLI GKGCMC 3 cut(s) 884, 1755, 1840
BstV1I GCAGC 4 cut(s) 1502, 1662, 1782, 1979
BstV2I GAAGAC 4 cut(s) 221, 360, 382, 1289
BstX2I RGATCY 2 cut(s) 1223, 1321
BstYI RGATCY 2 cut(s) 1223, 1321
BstZI CGGCCG 1 cut(s) 2041
BtgI CCRYGG 3 cut(s) 655, 1980, 2073
BtgZI GCGATG 3 cut(s) 1105, 1307, 1504
BtrI CACGTC 6 cut(s) 357, 754, 907, 922, 1278, 1402
BtsCI GGATG 2 cut(s) 501, 1705
BtsI GCAGTG 2 cut(s) 746, 1412
BtsIMutI CAGTG 7 cut(s) 746, 1101, 1412, 1494, 1750, 1771, 1833
BtuMI TCGCGA 1 cut(s) 1124
Cac8I GCNNGC 8 cut(s) 425, 472, 665, 679, 1164, 1525, 1888, 1904
CaiI CAGNNNCTG 1 cut(s) 1477
CciNI GCGGCCGC 1 cut(s) 2041
CfoI GCGC 7 cut(s) 177, 401, 1477, 1549, 1774, 1967, 2102
Cfr10I RCCGGY 3 cut(s) 274, 423, 950
Cfr42I CCGCGG 2 cut(s) 658, 1983
CpoI CGGWCCG 1 cut(s) 374
Csp6I GTAC 9 cut(s) 163, 780, 1153, 1237, 1336, 1465, 1720, 1930, 2107
CspI CGGWCCG 1 cut(s) 374
CviAII CATG 5 cut(s) 466, 758, 1528, 1690, 1921
CviQI GTAC 9 cut(s) 163, 780, 1153, 1237, 1336, 1465, 1720, 1930, 2107
DdeI CTNAG 6 cut(s) 170, 912, 1040, 1486, 1716, 1799
DriI GACNNNNNGTC 2 cut(s) 828, 1612
EaeI YGGCCR 3 cut(s) 351, 1263, 2041
EagI CGGCCG 1 cut(s) 2041
Eam1104I CTCTTC 1 cut(s) 597
Eam1105I GACNNNNNGTC 2 cut(s) 828, 1612
EarI CTCTTC 1 cut(s) 597
EciI GGCGGA 2 cut(s) 609, 1720
Ecl136II GAGCTC 3 cut(s) 688, 694, 1371
EclXI CGGCCG 1 cut(s) 2041
Eco130I CCWWGG 1 cut(s) 1960
Eco24I GRGCYC 6 cut(s) 690, 696, 936, 1373, 1755, 1840
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 5 cut(s) 313, 374, 573, 769, 801
Eco52I CGGCCG 1 cut(s) 2041
Eco53kI GAGCTC 3 cut(s) 688, 694, 1371
Eco57I CTGAAG 2 cut(s) 803, 1928
Eco72I CACGTG 1 cut(s) 430
Eco88I CYCGRG 3 cut(s) 557, 689, 935
EcoICRI GAGCTC 3 cut(s) 688, 694, 1371
EcoO109I RGGNCCY 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 1217
EcoRII CCWGG 6 cut(s) 579, 884, 1470, 1733, 1774, 1846
EcoT14I CCWWGG 1 cut(s) 1960
EcoT38I GRGCYC 6 cut(s) 690, 696, 936, 1373, 1755, 1840
ErhI CCWWGG 1 cut(s) 1960
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 5 cut(s) 469, 761, 1531, 1693, 1924
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 7 cut(s) 457, 539, 559, 778, 902, 917, 1700
FatI CATG 5 cut(s) 465, 757, 1527, 1689, 1920
FauI CCCGC 4 cut(s) 1178, 1369, 1610, 1895
FblI GTMKAC 3 cut(s) 381, 618, 1950
FokI GGATG 2 cut(s) 508, 1692
FriOI GRGCYC 6 cut(s) 690, 696, 936, 1373, 1755, 1840
FspBI CTAG 1 cut(s) 1436
GlaI GCGC 7 cut(s) 176, 400, 1476, 1548, 1773, 1966, 2101
HaeII RGCGCY 2 cut(s) 178, 1478
HhaI GCGC 7 cut(s) 177, 401, 1477, 1549, 1774, 1967, 2102
Hin1I GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
Hin1II CATG 5 cut(s) 469, 761, 1531, 1693, 1924
Hin6I GCGC 7 cut(s) 175, 399, 1475, 1547, 1772, 1965, 2100
HinP1I GCGC 7 cut(s) 175, 399, 1475, 1547, 1772, 1965, 2100
HincII GTYRAC 4 cut(s) 382, 833, 865, 1455
HindII GTYRAC 4 cut(s) 382, 833, 865, 1455
HphI GGTGA 2 cut(s) 806, 2105
Hpy188III TCNNGA 7 cut(s) 307, 536, 559, 839, 914, 1123, 1576
HpyAV CCTTC 6 cut(s) 304, 516, 638, 827, 1075, 1321
HpyCH4III ACNGT 3 cut(s) 625, 1096, 2111
HpyCH4V TGCA 8 cut(s) 667, 751, 882, 1357, 1405, 1410, 1518, 1527
HpyF3I CTNAG 6 cut(s) 170, 912, 1040, 1486, 1716, 1799
Hsp92I GRCGYC 7 cut(s) 226, 714, 1482, 1583, 1852, 1947, 2049
Hsp92II CATG 5 cut(s) 469, 761, 1531, 1693, 1924
HspAI GCGC 7 cut(s) 175, 399, 1475, 1547, 1772, 1965, 2100
KflI GGGWCCC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 423
KroNI GCCGGC 1 cut(s) 425
KspI CCGCGG 2 cut(s) 658, 1983
LmnI GCTCC 5 cut(s) 1033, 1427, 1562, 1707, 1974
Lsp1109I GCAGC 4 cut(s) 1502, 1662, 1782, 1979
LweI GCATC 2 cut(s) 1128, 1527
MaeI CTAG 1 cut(s) 1436
MaeIII GTNAC 4 cut(s) 836, 907, 1766, 2111
MbiI CCGCTC 1 cut(s) 708
MflI RGATCY 2 cut(s) 1223, 1321
MhlI GDGCHC 8 cut(s) 690, 696, 884, 936, 1373, 1755, 1840, 1979
MlsI TGGCCA 2 cut(s) 353, 1265
MluI ACGCGT 1 cut(s) 891
MluNI TGGCCA 2 cut(s) 353, 1265
MlyI GAGTC 5 cut(s) 465, 535, 904, 947, 1947
MmeI TCCRAC 2 cut(s) 1115, 1611
Mox20I TGGCCA 2 cut(s) 353, 1265
MroNI GCCGGC 1 cut(s) 423
MroXI GAANNNNTTC 1 cut(s) 1997
MscI TGGCCA 2 cut(s) 353, 1265
MseI TTAA 2 cut(s) 1104, 1392
MslI CAYNNNNRTG 1 cut(s) 756
Msp20I TGGCCA 2 cut(s) 353, 1265
MspA1I CMGCKG 3 cut(s) 657, 1254, 1982
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 6 cut(s) 581, 886, 1472, 1735, 1776, 1848
NaeI GCCGGC 1 cut(s) 425
NciI CCSGG 9 cut(s) 763, 855, 902, 931, 1197, 1432, 1505, 1610, 1883
NgoMIV GCCGGC 1 cut(s) 423
NlaIII CATG 5 cut(s) 469, 761, 1531, 1693, 1924
NlaIV GGNNCC 9 cut(s) 574, 575, 771, 1035, 1225, 1323, 1429, 1753, 1838
NmeAIII GCCGAG 3 cut(s) 375, 1641, 1923
NmuCI GTSAC 4 cut(s) 836, 907, 1766, 2111
NotI GCGGCCGC 1 cut(s) 2041
NruI TCGCGA 1 cut(s) 1124
NspI RCATGY 1 cut(s) 1693
NspV TTCGAA 1 cut(s) 283
PaeR7I CTCGAG 3 cut(s) 557, 689, 935
PciI ACATGT 1 cut(s) 1689
PcsI WCGNNNNNNNCGW 8 cut(s) 362, 597, 621, 681, 750, 978, 1008, 1011
PctI GAATGC 1 cut(s) 118
PdiI GCCGGC 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 1997
PfeI GAWTC 8 cut(s) 135, 219, 285, 322, 584, 858, 1200, 2035
PflFI GACNNNGTC 1 cut(s) 386
PflMI CCANNNNNTGG 2 cut(s) 508, 1477
PfoI TCCNGGA 1 cut(s) 1503
PleI GAGTC 5 cut(s) 464, 535, 904, 946, 1946
PmaCI CACGTG 1 cut(s) 430
PmlI CACGTG 1 cut(s) 430
PpsI GAGTC 5 cut(s) 464, 535, 904, 946, 1946
Ppu21I YACGTR 2 cut(s) 430, 1156
PpuMI RGGWCCY 1 cut(s) 573
PscI ACATGT 1 cut(s) 1689
PshAI GACNNNNGTC 1 cut(s) 1571
PsiI TTATAA 1 cut(s) 41
Psp124BI GAGCTC 3 cut(s) 690, 696, 1373
Psp1406I AACGTT 1 cut(s) 1245
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 6 cut(s) 579, 884, 1470, 1733, 1774, 1846
PspCI CACGTG 1 cut(s) 430
PspGI CCWGG 6 cut(s) 579, 884, 1470, 1733, 1774, 1846
PspN4I GGNNCC 9 cut(s) 574, 575, 771, 1035, 1225, 1323, 1429, 1753, 1838
PspOMI GGGCCC 2 cut(s) 1751, 1836
PspPPI RGGWCCY 1 cut(s) 573
PspXI VCTCGAGB 2 cut(s) 689, 935
PstI CTGCAG 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 1477
PsuI RGATCY 2 cut(s) 1223, 1321
PsyI GACNNNGTC 1 cut(s) 386
RruI TCGCGA 1 cut(s) 1124
RsaI GTAC 9 cut(s) 164, 781, 1154, 1238, 1337, 1466, 1721, 1931, 2108
RsaNI GTAC 9 cut(s) 163, 780, 1153, 1237, 1336, 1465, 1720, 1930, 2107
RseI CAYNNNNRTG 1 cut(s) 756
Rsr2I CGGWCCG 1 cut(s) 374
RsrII CGGWCCG 1 cut(s) 374
SacI GAGCTC 3 cut(s) 690, 696, 1373
SacII CCGCGG 2 cut(s) 658, 1983
SalI GTCGAC 1 cut(s) 380
SaqAI TTAA 2 cut(s) 1104, 1392
SbfI CCTGCAGG 1 cut(s) 669
SchI GAGTC 5 cut(s) 465, 535, 904, 947, 1947
SdaI CCTGCAGG 1 cut(s) 669
SduI GDGCHC 8 cut(s) 690, 696, 884, 936, 1373, 1755, 1840, 1979
SfaNI GCATC 2 cut(s) 1128, 1527
SfcI CTRYAG 1 cut(s) 665
Sfr274I CTCGAG 3 cut(s) 557, 689, 935
Sfr303I CCGCGG 2 cut(s) 658, 1983
SfuI TTCGAA 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 950
SgrBI CCGCGG 2 cut(s) 658, 1983
SinI GGWCC 5 cut(s) 313, 374, 573, 769, 801
SlaI CTCGAG 3 cut(s) 557, 689, 935
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 5 cut(s) 536, 557, 689, 935, 1366
SmoI CTYRAG 5 cut(s) 536, 557, 689, 935, 1366
Sse8387I CCTGCAGG 1 cut(s) 669
SspMI CTAG 1 cut(s) 1436
SstI GAGCTC 3 cut(s) 690, 696, 1373
StyI CCWWGG 1 cut(s) 1960
TaaI ACNGT 3 cut(s) 625, 1096, 2111
TaqI TCGA 9 cut(s) 283, 381, 558, 642, 690, 936, 981, 1449, 1791
TatI WGTACW 4 cut(s) 162, 1335, 1929, 2106
TauI GCSGC 9 cut(s) 711, 950, 1011, 1039, 1637, 1715, 2043, 2046, 2072
TfiI GAWTC 8 cut(s) 135, 219, 285, 322, 584, 858, 1200, 2035
Tru1I TTAA 2 cut(s) 1104, 1392
Tru9I TTAA 2 cut(s) 1104, 1392
TscAI CASTG 7 cut(s) 753, 1101, 1412, 1494, 1750, 1771, 1833
TseFI GTSAC 4 cut(s) 836, 907, 1766, 2111
TseI GCWGC 4 cut(s) 1515, 1675, 1795, 1967
Tsp45I GTSAC 4 cut(s) 836, 907, 1766, 2111
TspDTI ATGAA 4 cut(s) 50, 454, 1247, 1937
TspGWI ACGGA 7 cut(s) 310, 782, 793, 803, 988, 1301, 2002
TspRI CASTG 7 cut(s) 753, 1101, 1412, 1494, 1750, 1771, 1833
Tth111I GACNNNGTC 1 cut(s) 386
Van91I CCANNNNNTGG 2 cut(s) 508, 1477
VneI GTGCAC 1 cut(s) 880
VpaK11BI GGWCC 5 cut(s) 313, 374, 573, 769, 801
XapI RAATTY 6 cut(s) 10, 403, 1063, 1217, 1340, 1498
XceI RCATGY 1 cut(s) 1693
XcmI CCANNNNNNNNNTGG 1 cut(s) 1258
XhoI CTCGAG 3 cut(s) 557, 689, 935
XmiI GTMKAC 3 cut(s) 381, 618, 1950
XmnI GAANNNNTTC 1 cut(s) 1997
XspI CTAG 1 cut(s) 1436
ZraI GACGTC 1 cut(s) 2050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.