Rh2BG030600

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009084
Physical Location & Seq
Forward (+)
2040129 .. 2043348
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG030600.1

Sequence Viewer

Length: 2193 bp
ATGACGATTAAGCCGGCGGTTCGCGTTTCCGACGGGAAACTGATGGTGAAGAACCGGACGGTTCTGACCGGATTACCGGACAATGTGGTCGCCACGTCCGGTTCGGCTTCCGGTCCCGCCGAGGGAGTCTTCCTCGGCGCCGCGTTCGAGGAGGGCAGCAGCCGGCACGTGACTTCTCTCGGAACTTTACAAAACGGCGTGCGTTTCATGGCGTGCTTCCGGTTCAAGCTATGGTGGATGGCCCAGAAAATGGGCCGCTCCGGCCGCGACGTCCCTCTCGAGACTCAATTCCTATTGGTCGAGACCCAAGAAGACGGGTCCCACCTCGAAGAGGACCAGATCGTGTACGCCGTGTTCCTCCCTCTTATCGAAGGCCCCTTCAGGGCTAGCTTACAGGGGAACAGCGGAGACGAGCTGGAGCTTTGTTTGGAGAGCGGCGACGTGGACACCAAAGCGGCGTCGTTTAGCCACGCGGTGTTCGTTCACGCAGGGACGGACCCGTTCGCGACGATAACGGAGGCCGTCAGGGCCGTTAAGGTACACCTGAAGACGTTCCGTCAACGTCACGAGAAGAAGCTCCCCGGAATCGTTGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCAGGAAGTGACTCAGGAAGGAGTTGAGGCCGGCCTCAAGTCCCTCACCGCCGGCGGAGCGCCGCCGAAGTTTGTCATCATCGACGACGGGTGGCAGTCAGTCGCCGGCGATCAGGACAAAGCCGGCGAGCTACAGAGACTGACCGGAATCAAAGAAAACTCGAAGTTTCAACACAAGGATGATGGGATAATGACGATAGTGAACATAGCAAAAGAAAAATACGGGTTGAAATACGTGTATGTCTGGCACGCGATTACCGGCTACTGGGGAGGAGTCCGGCCCGGAACTGAATACGGGTCGGTTCTGAAGTACCCGGATTTGTCGAAAGGGGTTATGGAAAACGAACCGGCCTGGAAAACGGACATGATCCGGTTACAGGGTCTGGGCTTAGTGAGCCCGAAAAGCGTGTACAAGTTTTACAACGAGCTACACAGCTACTTGGCCTCTGCGGGTGTAGATGGGGTTAAGGTGGACGTGCAGTGCGTGCTGGAGACTCTCGGAGCCGGGCTGGGCGGGCGGGTCGAGCTGACCCGGCAGTACCACCAGGCATTGGATGCTTCGGTGTCTCGGAACTTTGAAGATAATGGGATCATAGCATGCATGAGCCATAATACTGATGCGCTGTACTGTGCGAAACAGACGGCGGTGGTGAGAGCCTCCGACGATTTCTACCCGCGCGATCCGAAATCGCATACGATCCACGTGGCGGCGGTGGCGTATAATAGCGTTTTCCTCGGCGAGTTTATGCTGCCGGACTGGGATATGTTCCACTCGCTCCACCCCGCGGCTGAGTACCACGCGTCCGCTAGAGCCATTAGTGGTGGACCTGTTTATGTTAGTGATGCGCCTGGGAAGCATGACTTTGAGCTTTTGAAGAAGATAGTATTGCCGGACGGGTCGGTGCTCCGGGCGCGATTACCCGGAAGGCCGACCCGGGACTGCTTGTTCACTGACCCGGCCCGGGATGGGGTGAGTTTGTTGAAGATATGGAACATGAATAAGTACACAGGTGTAGTGGGGGTGTACAATTGCCAAGGAGCAGCATGGAGTTCACTGGAGAGAAAGAATGCATTCCACGAGACGAAATCGGACGCCATTACCGGTTATGTTCGCGGCCGTGACGTCCACCTCATTAAGGAGGCAGCCACTGATCCCGATTGGAATGGTGACTGCGCCGTGTATTGCAATCGGACAGGGGAGCTTGTGACATTGCCTCACAATGCAGCCATGCCTATCTCTCTCAAGGTGCTTGAACACGAGATTTTCACTGTGAGTCCCATCAGGGTTTTGGCGCCTGGCGTGAGATTCGCTCCTTTGGGACTGGTTGACATGTACAATGCCGGTGGAGCCATTGACGACTTGAGATATGAGCAGAGATTAGTCTCTATGGAGGTTAGAGGGTGTGGCAAGTTTGGTGTGTATTCTTCAGAGAAGCCCAGGAGGTGTTGTGTAGGGACTAATGGGGTTGATTTCAGCTATGATTCGGCATCTGGGTTGGTGACTTTGAGCTTGGATCATATGCCTGAAGAGGGAAAAAGGGTACAGCCAGTCGAGGTTGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

730

Amino Acids

80.14

Weight (kDa)

5.96

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 712 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 273, 1752
AccB1I GGYRCC 2 cut(s) 137, 1918
AccB7I CCANNNNNTGG 2 cut(s) 250, 1177
AccBSI CCGCTC 2 cut(s) 258, 435
AclWI GGATC 6 cut(s) 988, 1223, 1301, 1318, 1772, 2148
AcoI YGGCCR 2 cut(s) 262, 1741
AcuI CTGAAG 5 cut(s) 364, 566, 953, 2037, 2172
AcvI CACGTG 2 cut(s) 169, 1330
AcyI GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
AdeI CACNNNGTG 1 cut(s) 475
AflIII ACRYGT 4 cut(s) 618, 861, 1425, 1956
AgeI ACCGGT 1 cut(s) 1727
AgsI TTSAA 7 cut(s) 226, 797, 856, 1205, 1501, 1609, 1880
AhdI GACNNNNNGTC 1 cut(s) 555
AjiI CACGTC 3 cut(s) 96, 442, 1102
AjnI CCWGG 7 cut(s) 611, 627, 977, 1170, 1474, 1921, 2063
AloI GAACNNNNNNTCC 2 cut(s) 1556, 1588
Alw21I GWGCWC 2 cut(s) 611, 1533
Alw26I GTCTC 8 cut(s) 275, 296, 402, 757, 1112, 1197, 1700, 2014
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 6 cut(s) 988, 1223, 1301, 1318, 1772, 2148
AlwNI CAGNNNCTG 3 cut(s) 766, 1009, 1775
Ama87I CYCGRG 3 cut(s) 278, 1560, 1587
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 6 cut(s) 156, 159, 1375, 1667, 1769, 1850
AsiGI ACCGGT 1 cut(s) 1727
Asp700I GAANNNNTTC 2 cut(s) 551, 1697
AspLEI GCGC 8 cut(s) 140, 688, 1249, 1305, 1474, 1540, 1802, 1921
AsuHPI GGTGA 6 cut(s) 58, 664, 1288, 1609, 1805, 2137
AsuNHI GCTAGC 1 cut(s) 386
AvaI CYCGRG 3 cut(s) 278, 1560, 1587
AvaII GGWCC 5 cut(s) 113, 318, 334, 496, 1451
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 2 cut(s) 137, 1918
BanII GRGCYC 1 cut(s) 1025
BauI CACGAG 3 cut(s) 566, 1703, 1883
BbrPI CACGTG 2 cut(s) 169, 1330
BbsI GAAGAC 3 cut(s) 121, 318, 554
Bbv12I GWGCWC 2 cut(s) 611, 1533
BbvI GCAGC 6 cut(s) 168, 171, 1362, 1679, 1781, 1862
BccI CCATC 6 cut(s) 37, 232, 803, 1079, 1586, 1913
BceAI ACGGC 7 cut(s) 211, 335, 506, 515, 1284, 1728, 1787
BciT130I CCWGG 7 cut(s) 613, 629, 979, 1172, 1476, 1923, 2065
BcoDI GTCTC 8 cut(s) 275, 296, 402, 757, 1112, 1197, 1700, 2014
BfaI CTAG 2 cut(s) 387, 1434
BfmI CTRYAG 1 cut(s) 758
BfoI RGCGCY 3 cut(s) 141, 689, 1922
BglI GCCNNNNNGGC 2 cut(s) 261, 527
Bme18I GGWCC 5 cut(s) 113, 318, 334, 496, 1451
BmeRI GACNNNNNGTC 1 cut(s) 555
BmeT110I CYCGRG 3 cut(s) 278, 1560, 1587
BmgBI CACGTC 3 cut(s) 96, 442, 1102
BmiI GGNNCC 9 cut(s) 115, 139, 319, 320, 376, 498, 1129, 1920, 1975
BmrI ACTGGG 2 cut(s) 901, 1393
BmsI GCATC 4 cut(s) 1171, 1234, 1459, 2123
BmtI GCTAGC 1 cut(s) 390
BmuI ACTGGG 2 cut(s) 901, 1393
BpiI GAAGAC 3 cut(s) 121, 318, 554
BplI GAGNNNNNCTC 4 cut(s) 117, 149, 1921, 1953
BpmI CTGGAG 3 cut(s) 437, 1136, 1703
BpuEI CTTGAG 3 cut(s) 647, 1853, 2008
BsaAI YACGTR 3 cut(s) 169, 862, 1330
BsaHI GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
BsaI GGTCTC 1 cut(s) 296
BsaWI WCCGGW 9 cut(s) 54, 68, 76, 98, 110, 219, 770, 996, 1727
BsaXI ACNNNNNCTCC 2 cut(s) 2059, 2089
Bse1I ACTGG 5 cut(s) 896, 1388, 1686, 1953, 2174
Bse3DI GCAATG 1 cut(s) 1835
BseBI CCWGG 7 cut(s) 613, 629, 979, 1172, 1476, 1923, 2065
BseGI GGATG 4 cut(s) 243, 811, 1186, 1597
BseMI GCAATG 1 cut(s) 1835
BseMII CTCAG 2 cut(s) 653, 1407
BseNI ACTGG 5 cut(s) 896, 1388, 1686, 1953, 2174
BseRI GAGGAG 2 cut(s) 164, 912
BseSI GKGCMC 1 cut(s) 611
BseX3I CGGCCG 2 cut(s) 262, 1741
BseXI GCAGC 6 cut(s) 168, 171, 1362, 1679, 1781, 1862
BseYI CCCAGC 1 cut(s) 1135
BsgI GTGCAG 1 cut(s) 1124
Bsh1285I CGRYCG 2 cut(s) 265, 1744
BshNI GGYRCC 2 cut(s) 137, 1918
BshTI ACCGGT 1 cut(s) 1727
BsiEI CGRYCG 2 cut(s) 265, 1744
BsiHKAI GWGCWC 2 cut(s) 611, 1533
BsiHKCI CYCGRG 3 cut(s) 278, 1560, 1587
BsmAI GTCTC 8 cut(s) 275, 296, 402, 757, 1112, 1197, 1700, 2014
BsmBI CGTCTC 2 cut(s) 402, 1700
BsmI GAATGC 2 cut(s) 1697, 1699
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 3 cut(s) 278, 1560, 1587
Bsp1286I GDGCHC 3 cut(s) 611, 1025, 1533
Bsp1407I TGTACA 3 cut(s) 1035, 1650, 1959
Bsp143I GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
Bsp68I TCGCGA 1 cut(s) 506
BspCNI CTCAG 2 cut(s) 652, 1408
BspLI GGNNCC 9 cut(s) 115, 139, 319, 320, 376, 498, 1129, 1920, 1975
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 6 cut(s) 988, 1223, 1301, 1318, 1772, 2148
BspT107I GGYRCC 2 cut(s) 137, 1918
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 2 cut(s) 258, 435
BsrDI GCAATG 1 cut(s) 1835
BsrGI TGTACA 3 cut(s) 1035, 1650, 1959
BsrI ACTGG 5 cut(s) 896, 1388, 1686, 1953, 2174
BssMI GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
BssNI GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
BssSI CACGAG 3 cut(s) 566, 1703, 1883
BssT1I CCWWGG 1 cut(s) 1660
Bst2BI CACGAG 3 cut(s) 566, 1703, 1883
Bst2UI CCWGG 7 cut(s) 613, 629, 979, 1172, 1476, 1923, 2065
Bst4CI ACNGT 3 cut(s) 61, 1256, 1897
Bst6I CTCTTC 2 cut(s) 324, 2148
BstACI GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
BstAPI GCANNNNNTGC 2 cut(s) 1111, 1181
BstAUI TGTACA 3 cut(s) 1035, 1650, 1959
BstBAI YACGTR 3 cut(s) 169, 862, 1330
BstDEI CTNAG 3 cut(s) 639, 1015, 1416
BstDSI CCRYGG 1 cut(s) 1410
BstENI CCTNNNNNAGG 2 cut(s) 329, 1760
BstF5I GGATG 4 cut(s) 243, 811, 1186, 1597
BstH2I RGCGCY 3 cut(s) 141, 689, 1922
BstHHI GCGC 8 cut(s) 140, 688, 1249, 1305, 1474, 1540, 1802, 1921
BstKTI GATC 8 cut(s) 342, 739, 996, 1218, 1309, 1326, 1780, 2143
BstMAI GTCTC 8 cut(s) 275, 296, 402, 757, 1112, 1197, 1700, 2014
BstMBI GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
BstMCI CGRYCG 2 cut(s) 265, 1744
BstNI CCWGG 7 cut(s) 613, 629, 979, 1172, 1476, 1923, 2065
BstNSI RCATGY 2 cut(s) 1227, 1960
BstSFI CTRYAG 1 cut(s) 758
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 6 cut(s) 168, 171, 1362, 1679, 1781, 1862
BstV2I GAAGAC 3 cut(s) 121, 318, 554
BstZI CGGCCG 2 cut(s) 262, 1741
BtgI CCRYGG 1 cut(s) 1410
BtrI CACGTC 3 cut(s) 96, 442, 1102
BtsCI GGATG 4 cut(s) 243, 811, 1186, 1597
BtsI GCAGTG 1 cut(s) 1112
BtsIMutI CAGTG 5 cut(s) 1112, 1575, 1679, 1773, 1893
BtuMI TCGCGA 1 cut(s) 506
CaiI CAGNNNCTG 3 cut(s) 766, 1009, 1775
CfoI GCGC 8 cut(s) 140, 688, 1249, 1305, 1474, 1540, 1802, 1921
Cfr42I CCGCGG 1 cut(s) 1413
Cfr9I CCCGGG 2 cut(s) 1560, 1587
CseI GACGC 4 cut(s) 447, 626, 1416, 1727
CspAI ACCGGT 1 cut(s) 1727
CspCI CAANNNNNGTGG 2 cut(s) 1951, 1986
CviAII CATG 9 cut(s) 208, 991, 1224, 1228, 1484, 1621, 1671, 1855, 1957
DdeI CTNAG 3 cut(s) 639, 1015, 1416
DinI GGCGCC 2 cut(s) 139, 1920
DpnI GATC 8 cut(s) 341, 738, 995, 1217, 1308, 1325, 1779, 2142
DpnII GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
DraIII CACNNNGTG 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 262, 1741
EagI CGGCCG 2 cut(s) 262, 1741
Eam1104I CTCTTC 2 cut(s) 324, 2148
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 2 cut(s) 324, 2148
EciI GGCGGA 1 cut(s) 696
EclXI CGGCCG 2 cut(s) 262, 1741
Eco130I CCWWGG 1 cut(s) 1660
Eco24I GRGCYC 1 cut(s) 1025
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 5 cut(s) 113, 318, 334, 496, 1451
Eco52I CGGCCG 2 cut(s) 262, 1741
Eco57I CTGAAG 5 cut(s) 364, 566, 953, 2037, 2172
Eco72I CACGTG 2 cut(s) 169, 1330
Eco88I CYCGRG 3 cut(s) 278, 1560, 1587
EcoNI CCTNNNNNAGG 2 cut(s) 329, 1760
EcoO109I RGGNCCY 2 cut(s) 318, 374
EcoRII CCWGG 7 cut(s) 611, 627, 977, 1170, 1474, 1921, 2063
EcoT14I CCWWGG 1 cut(s) 1660
EcoT22I ATGCAT 2 cut(s) 1229, 1699
EcoT38I GRGCYC 1 cut(s) 1025
EgeI GGCGCC 2 cut(s) 139, 1920
EheI GGCGCC 2 cut(s) 139, 1920
ErhI CCWWGG 1 cut(s) 1660
Esp3I CGTCTC 2 cut(s) 402, 1700
FaeI CATG 9 cut(s) 211, 994, 1227, 1231, 1487, 1624, 1674, 1858, 1960
FalI AAGNNNNNCTT 2 cut(s) 1472, 1504
FatI CATG 9 cut(s) 207, 990, 1223, 1227, 1483, 1620, 1670, 1854, 1956
FauI CCCGC 6 cut(s) 124, 1069, 1133, 1137, 1308, 1417
FauNDI CATATG 1 cut(s) 2145
FokI GGATG 4 cut(s) 250, 818, 1193, 1604
FriOI GRGCYC 1 cut(s) 1025
FseI GGCCGGCC 1 cut(s) 660
FspBI CTAG 2 cut(s) 387, 1434
GlaI GCGC 8 cut(s) 139, 687, 1248, 1304, 1473, 1539, 1801, 1920
GsaI CCCAGC 1 cut(s) 1139
GsuI CTGGAG 3 cut(s) 437, 1136, 1703
HaeII RGCGCY 3 cut(s) 141, 689, 1922
HgaI GACGC 4 cut(s) 447, 626, 1416, 1727
HhaI GCGC 8 cut(s) 140, 688, 1249, 1305, 1474, 1540, 1802, 1921
Hin1I GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
Hin1II CATG 9 cut(s) 211, 994, 1227, 1231, 1487, 1624, 1674, 1858, 1960
Hin6I GCGC 8 cut(s) 138, 686, 1247, 1303, 1472, 1538, 1800, 1919
HinP1I GCGC 8 cut(s) 138, 686, 1247, 1303, 1472, 1538, 1800, 1919
HincII GTYRAC 3 cut(s) 560, 592, 1954
HindII GTYRAC 3 cut(s) 560, 592, 1954
HphI GGTGA 6 cut(s) 58, 664, 1288, 1609, 1805, 2137
Hpy188III TCNNGA 8 cut(s) 278, 280, 301, 505, 566, 641, 740, 1781
Hpy99I CGWCG 8 cut(s) 35, 272, 443, 463, 511, 713, 716, 1292
HpyAV CCTTC 4 cut(s) 365, 388, 638, 1545
HpyCH4III ACNGT 3 cut(s) 61, 1256, 1897
HpyCH4V TGCA 6 cut(s) 609, 1105, 1227, 1697, 1812, 1850
HpyF3I CTNAG 3 cut(s) 639, 1015, 1416
Hsp92I GRCGYC 6 cut(s) 138, 270, 458, 1719, 1749, 1919
Hsp92II CATG 9 cut(s) 211, 994, 1227, 1231, 1487, 1624, 1674, 1858, 1960
HspAI GCGC 8 cut(s) 138, 686, 1247, 1303, 1472, 1538, 1800, 1919
KasI GGCGCC 2 cut(s) 137, 1918
KflI GGGWCCC 1 cut(s) 318
KroI GCCGGC 6 cut(s) 13, 162, 656, 677, 731, 749
KroNI GCCGGC 6 cut(s) 15, 164, 658, 679, 733, 751
KspI CCGCGG 1 cut(s) 1413
Kzo9I GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
Lsp1109I GCAGC 6 cut(s) 168, 171, 1362, 1679, 1781, 1862
LweI GCATC 4 cut(s) 1171, 1234, 1459, 2123
MaeI CTAG 2 cut(s) 387, 1434
MaeIII GTNAC 8 cut(s) 169, 563, 634, 999, 1745, 1793, 1831, 2125
MalI GATC 8 cut(s) 341, 738, 995, 1217, 1308, 1325, 1779, 2142
MbiI CCGCTC 2 cut(s) 258, 435
MboI GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
MfeI CAATTG 1 cut(s) 1654
MhlI GDGCHC 3 cut(s) 611, 1025, 1533
MluCI AATT 2 cut(s) 287, 1654
MluI ACGCGT 2 cut(s) 618, 1425
Mly113I GGCGCC 2 cut(s) 138, 1919
MlyI GAGTC 6 cut(s) 135, 277, 631, 909, 1114, 1909
MmeI TCCRAC 2 cut(s) 54, 1311
Mph1103I ATGCAT 2 cut(s) 1229, 1699
MreI CGCCGGCG 2 cut(s) 677, 731
MroNI GCCGGC 6 cut(s) 13, 162, 656, 677, 731, 749
MroXI GAANNNNTTC 2 cut(s) 551, 1697
MseI TTAA 4 cut(s) 9, 534, 1092, 1761
MslI CAYNNNNRTG 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 405, 1412
MunI CAATTG 1 cut(s) 1654
Mva1269I GAATGC 2 cut(s) 1697, 1699
MvaI CCWGG 7 cut(s) 613, 629, 979, 1172, 1476, 1923, 2065
NaeI GCCGGC 6 cut(s) 15, 164, 658, 679, 733, 751
NarI GGCGCC 2 cut(s) 138, 1919
NdeI CATATG 1 cut(s) 2145
NdeII GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
NgoMIV GCCGGC 6 cut(s) 13, 162, 656, 677, 731, 749
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 9 cut(s) 211, 994, 1227, 1231, 1487, 1624, 1674, 1858, 1960
NlaIV GGNNCC 9 cut(s) 115, 139, 319, 320, 376, 498, 1129, 1920, 1975
NmeAIII GCCGAG 3 cut(s) 114, 145, 1341
NmuCI GTSAC 7 cut(s) 169, 563, 634, 1745, 1793, 1831, 2125
NruI TCGCGA 1 cut(s) 506
NsiI ATGCAT 2 cut(s) 1229, 1699
NspI RCATGY 2 cut(s) 1227, 1960
PaeI GCATGC 1 cut(s) 1227
PaeR7I CTCGAG 1 cut(s) 278
PciI ACATGT 1 cut(s) 1956
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 348, 477, 1107
PctI GAATGC 2 cut(s) 1697, 1699
PdiI GCCGGC 6 cut(s) 15, 164, 658, 679, 733, 751
PdmI GAANNNNTTC 2 cut(s) 551, 1697
PfeI GAWTC 4 cut(s) 585, 774, 1932, 2108
PflMI CCANNNNNTGG 2 cut(s) 250, 1177
PinAI ACCGGT 1 cut(s) 1727
PleI GAGTC 6 cut(s) 134, 277, 631, 908, 1114, 1908
PluTI GGCGCC 2 cut(s) 141, 1922
PmaCI CACGTG 2 cut(s) 169, 1330
PmlI CACGTG 2 cut(s) 169, 1330
PpsI GAGTC 6 cut(s) 134, 277, 631, 908, 1114, 1908
Ppu21I YACGTR 3 cut(s) 169, 862, 1330
PpuMI RGGWCCY 1 cut(s) 318
PscI ACATGT 1 cut(s) 1956
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 7 cut(s) 611, 627, 977, 1170, 1474, 1921, 2063
PspCI CACGTG 2 cut(s) 169, 1330
PspFI CCCAGC 1 cut(s) 1135
PspGI CCWGG 7 cut(s) 611, 627, 977, 1170, 1474, 1921, 2063
PspN4I GGNNCC 9 cut(s) 115, 139, 319, 320, 376, 498, 1129, 1920, 1975
PspPPI RGGWCCY 1 cut(s) 318
PsrI GAACNNNNNNTAC 2 cut(s) 338, 370
PstNI CAGNNNCTG 3 cut(s) 766, 1009, 1775
RigI GGCCGGCC 1 cut(s) 660
RruI TCGCGA 1 cut(s) 506
RseI CAYNNNNRTG 1 cut(s) 804
SacII CCGCGG 1 cut(s) 1413
SaqAI TTAA 4 cut(s) 9, 534, 1092, 1761
Sau3AI GATC 8 cut(s) 339, 736, 993, 1215, 1306, 1323, 1777, 2140
SchI GAGTC 6 cut(s) 135, 277, 631, 909, 1114, 1909
SduI GDGCHC 3 cut(s) 611, 1025, 1533
SfaNI GCATC 4 cut(s) 1171, 1234, 1459, 2123
SfcI CTRYAG 1 cut(s) 758
SfiI GGCCNNNNNGGCC 2 cut(s) 261, 527
SfoI GGCGCC 2 cut(s) 139, 1920
Sfr274I CTCGAG 1 cut(s) 278
Sfr303I CCGCGG 1 cut(s) 1413
SgrAI CRCCGGYG 2 cut(s) 677, 731
SgrBI CCGCGG 1 cut(s) 1413
SinI GGWCC 5 cut(s) 113, 318, 334, 496, 1451
SlaI CTCGAG 1 cut(s) 278
SmaI CCCGGG 2 cut(s) 1562, 1589
SmiMI CAYNNNNRTG 1 cut(s) 804
SmlI CTYRAG 4 cut(s) 278, 662, 1868, 1987
SmoI CTYRAG 4 cut(s) 278, 662, 1868, 1987
SphI GCATGC 1 cut(s) 1227
Sse9I AATT 2 cut(s) 287, 1654
SspDI GGCGCC 2 cut(s) 137, 1918
SspMI CTAG 2 cut(s) 387, 1434
StyI CCWWGG 1 cut(s) 1660
TaaI ACNGT 3 cut(s) 61, 1256, 1897
TasI AATT 2 cut(s) 287, 1654
TatI WGTACW 5 cut(s) 1035, 1251, 1629, 1650, 1959
TauI GCSGC 9 cut(s) 143, 258, 267, 438, 458, 691, 1337, 1415, 1743
TfiI GAWTC 4 cut(s) 585, 774, 1932, 2108
Tru1I TTAA 4 cut(s) 9, 534, 1092, 1761
Tru9I TTAA 4 cut(s) 9, 534, 1092, 1761
TscAI CASTG 5 cut(s) 1112, 1582, 1686, 1780, 1900
TseFI GTSAC 7 cut(s) 169, 563, 634, 1745, 1793, 1831, 2125
TseI GCWGC 6 cut(s) 156, 159, 1375, 1667, 1769, 1850
Tsp45I GTSAC 7 cut(s) 169, 563, 634, 1745, 1793, 1831, 2125
TspDTI ATGAA 2 cut(s) 196, 1637
TspGWI ACGGA 4 cut(s) 509, 530, 545, 1001
TspMI CCCGGG 2 cut(s) 1560, 1587
TspRI CASTG 5 cut(s) 1112, 1582, 1686, 1780, 1900
Van91I CCANNNNNTGG 2 cut(s) 250, 1177
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 5 cut(s) 113, 318, 334, 496, 1451
XagI CCTNNNNNAGG 2 cut(s) 329, 1760
XceI RCATGY 2 cut(s) 1227, 1960
XcmI CCANNNNNNNNNTGG 1 cut(s) 1912
XhoI CTCGAG 1 cut(s) 278
XmaI CCCGGG 2 cut(s) 1560, 1587
XmnI GAANNNNTTC 2 cut(s) 551, 1697
XspI CTAG 2 cut(s) 387, 1434
ZraI GACGTC 2 cut(s) 271, 1750
Zsp2I ATGCAT 2 cut(s) 1229, 1699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.