Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Forward (+)

6876441 .. 6881506

Gene Structure 5066 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G20350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGTCATCGGAGATTGAGGTGGTGGAAGAAATCCAATCGAATCCCAAGGAGAATGGGGAATCGAGTTCTAAAGGGATTGAAGAAGAGAGCTTGAAGAACGATGTCTATACTGCTGCCGCTTATGGTGATTTGGAGAAGCTTCATAGATTGGTTGAGTGTGAGGGTTCCTCTGTTTCCGAGCCTGATGCTCTTGGCTACTATGCTCTTCAGTGGTCTGCCTTGAACAACCGCGTCGCCGTTGCCCAGTACCTTATCGAGCATGGTGGAGATGTAAACGCGACGGATCATACTGGACAGACTGCATTGCATTGGAGTGCTGTTCGTGGTGCGATACAAGTTGCAGAACTGTTATTACAAGAGGGTGCAAGAGTAGATGCAACGGATATGTATGGCTATCAGGCAACACATGTTGCCGCACAGTATGGCCAGACTGCTTTTCTTTGTCACGTTGTCTCAAAGTGGAATGCTGATCCTGACGTGCCTGATAATGATGGAAGAAGCCCCTTGCATTGGGCTGCATATAAAGGTTTTGCAGATTCCATTCGCCTACTTTTATTTCTAGATGCATATAGAGGACGACAAGACAAAGAAGGTTGCACTCCTCTGCATTGGGCTGCCATCCGAGGTAATTTGGAGGCTTGCACTGTCTTGGTGCAGGCTGGAAAGAAAGAGGATTTGATGATTACTGACAAGACTGGGCTAACCCCTGCGCAACTTGCTGCTGAAAAGAATCACAGACAAGTTTCTTTTTTCCTTGGTAATGCTCGAAGCCTGCTTGAAAAGCGTTGTGACGGAAGCAGTCCCCTCGGAAGATTGTCAAAGTTGGGACTCGCTCCAGTTCTTTGGATCATGATCCTGCTGCTTCTGCTCGTATATACGAATTCGGTTGTTTTGGCATCCAATCTGCCAAAGTTAACGACTGGGATTGGTGCGCTTGCATGGCTGGGATTCATTCTTGCAACTGCAGGATTATTTTTGTTCTACCGCTGTAGCAGGAAGGATCCAGGTTACATCAGAATGAATATCCATGATCCGCAAACCATGAAAGATGATGAACCACTCTTAAAAATAGAGCTAAACAATCCTGCTTTGCTTGCTGGGAATTGGACACAGCTCTGTGCAACATGCAAGATTATCAGACCTCTTCGAGCTAAGCACTGTTCCACCTGTGATCGTTGTGTAGAGCAATTTGATCACCATTGTCCTTGGGTATCAAATTGTGTTGGAAAAAAAAACAAGTGGGAATTTTTTCTTTTTCTTCTACTTGAAGTTCTAGCGATGCTGATAACTGGTGGCGTCACTCTTGCAAGAGTCTTGAGTGACCCTTCAGCTCCATCTTCTTTTGGAGCATGGATGAGCCATGTCGCTAGTAATCATGTGGGTGCTTTATCCTTTCTGCTAGTGGAATTCTGCCTCTTCTTTTCTGTCGCCGTCTTAACAGTCATACAAGCGTCACAAATATCAAGGAACATAACCACAAATGAAATGGCCAACGCCCTGCGGTACAGCTACCTAAGAGGTCCAGGTGGTCGGTTTAGGAATCCTTATGATCTCGGGTGCAGAAGAAACTGCTCGGACTTCTTAGTAAAAGGTTATAACGAAGATATTGAGTGTCATGAAGAAGACGCAACACAGAGACCAGAGGGTATTAGCATGATGCAGATGCAGCGGAATCCAAATCTTCAAAATGGCAATGGCCATGTTGCTATCGATGTTAACCCGACACATAATTCACAGTCAGCACATGTTCATTCTGCCAACTGCAGCCATAGCCATAATAGTAAGTCAAAGAGTGACAATGTTCCTTTGGGTTTGGGACTTGGTCTTAGCCGCAACCCAACCCGACCTGTTGTATCTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

620

Amino Acids

68.23

Weight (kDa)

6.34

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	37 - 128	7.8e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	76 - 118	1.3e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	136 - 222	2.3e-14	Ankyrin repeats (3 copies)
DHHC	PF01529	371 - 498	1.2e-31	DHHC palmitoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1596
Acc16I	TGCGCA	1 cut(s)	711
AccII	CGCG	2 cut(s)	231, 278
AciI	CCGC	8 cut(s)	117, 229, 414, 985, 1034, 1501, 1669, 1831
AclWI	GGATC	7 cut(s)	291, 464, 847, 854, 995, 1008, 1025
AcoI	YGGCCR	3 cut(s)	424, 1488, 1696
AcsI	RAATTY	3 cut(s)	880, 1244, 1406
AcuI	CTGAAG	2 cut(s)	191, 1311
AcyI	GRCGYC	1 cut(s)	1296
AfaI	GTAC	2 cut(s)	248, 1505
AfiI	CCNNNNNNNGG	1 cut(s)	510
AflIII	ACRYGT	2 cut(s)	406, 1744
AgsI	TTSAA	6 cut(s)	80, 94, 223, 779, 1268, 1685
AjiI	CACGTC	1 cut(s)	478
AjnI	CCWGG	2 cut(s)	1003, 1522
AluBI	AGCT	7 cut(s)	90, 139, 1075, 1114, 1151, 1331, 1509
AluI	AGCT	7 cut(s)	90, 139, 1075, 1114, 1151, 1331, 1509
Alw26I	GTCTC	2 cut(s)	457, 1630
AlwI	GGATC	7 cut(s)	291, 464, 847, 854, 995, 1008, 1025
Ama87I	CYCGRG	1 cut(s)	1553
AoxI	GGCC	3 cut(s)	424, 1488, 1696
ApeKI	GCWGC	7 cut(s)	113, 515, 614, 719, 859, 1666, 1764
ApoI	RAATTY	3 cut(s)	880, 1244, 1406
Asp700I	GAANNNNTTC	1 cut(s)	1248
AspLEI	GCGC	2 cut(s)	712, 934
AspS9I	GGNCC	1 cut(s)	1520
AsuHPI	GGTGA	2 cut(s)	137, 1187
AvaI	CYCGRG	1 cut(s)	1553
AvaII	GGWCC	1 cut(s)	1520
BalI	TGGCCA	3 cut(s)	426, 1490, 1698
BamHI	GGATCC	1 cut(s)	1000
BbsI	GAAGAC	1 cut(s)	1629
BbvI	GCAGC	7 cut(s)	100, 502, 601, 706, 846, 1678, 1776
BccI	CCATC	3 cut(s)	485, 626, 1342
BceAI	ACGGC	2 cut(s)	221, 1415
BcgI	CGANNNNNNTGC	4 cut(s)	167, 201, 787, 821
BciT130I	CCWGG	2 cut(s)	1005, 1524
BclI	TGATCA	1 cut(s)	1192
BcoDI	GTCTC	2 cut(s)	457, 1630
BfaI	CTAG	4 cut(s)	560, 1274, 1368, 1400
BfmI	CTRYAG	3 cut(s)	963, 988, 1762
BlpI	GCTNAGC	1 cut(s)	1152
Bme1390I	CCNGG	2 cut(s)	1005, 1524
Bme18I	GGWCC	1 cut(s)	1520
BmeT110I	CYCGRG	1 cut(s)	1553
BmgBI	CACGTC	1 cut(s)	478
BmgT120I	GGNCC	1 cut(s)	1520
BmiI	GGNNCC	2 cut(s)	166, 1002
BmrFI	CCNGG	2 cut(s)	1005, 1524
BmrI	ACTGGG	3 cut(s)	238, 705, 930
BmsI	GCATC	7 cut(s)	175, 364, 553, 905, 1269, 1647, 1653
BmuI	ACTGGG	3 cut(s)	238, 705, 930
BpiI	GAAGAC	1 cut(s)	1629
BplI	GAGNNNNNCTC	2 cut(s)	152, 184
BpmI	CTGGAG	1 cut(s)	819
Bpu1102I	GCTNAGC	1 cut(s)	1152
BpuEI	CTTGAG	1 cut(s)	1336
Bsa29I	ATCGAT	1 cut(s)	1710
BsaBI	GATNNNNATC	1 cut(s)	851
BsaHI	GRCGYC	1 cut(s)	1296
BsaI	GGTCTC	1 cut(s)	1630
BsaJI	CCNNGG	5 cut(s)	45, 622, 754, 805, 1205
Bsc4I	CCNNNNNNNGG	1 cut(s)	510
Bse1I	ACTGG	6 cut(s)	244, 295, 700, 836, 925, 1294
Bse3DI	GCAATG	2 cut(s)	302, 1699
Bse8I	GATNNNNATC	1 cut(s)	851
BseBI	CCWGG	2 cut(s)	1005, 1524
BseCI	ATCGAT	1 cut(s)	1710
BseDI	CCNNGG	5 cut(s)	45, 622, 754, 805, 1205
BseGI	GGATG	3 cut(s)	618, 896, 1359
BseJI	GATNNNNATC	1 cut(s)	851
BseLI	CCNNNNNNNGG	1 cut(s)	510
BseMI	GCAATG	2 cut(s)	302, 1699
BseNI	ACTGG	6 cut(s)	244, 295, 700, 836, 925, 1294
BseRI	GAGGAG	1 cut(s)	591
BseXI	GCAGC	7 cut(s)	100, 502, 601, 706, 846, 1678, 1776
BseYI	CCCAGC	2 cut(s)	943, 1097
BsgI	GTGCAG	2 cut(s)	674, 1579
Bsh1236I	CGCG	2 cut(s)	231, 278
BshFI	GGCC	3 cut(s)	426, 1490, 1698
BshVI	ATCGAT	1 cut(s)	1710
BsiHKCI	CYCGRG	1 cut(s)	1553
BslFI	GGGAC	3 cut(s)	786, 840, 1830
BslI	CCNNNNNNNGG	1 cut(s)	510
BsmAI	GTCTC	2 cut(s)	457, 1630
BsmFI	GGGAC	3 cut(s)	786, 840, 1830
BsmI	GAATGC	1 cut(s)	469
BsnI	GGCC	3 cut(s)	426, 1490, 1698
Bso31I	GGTCTC	1 cut(s)	1630
BsoBI	CYCGRG	1 cut(s)	1553
Bsp143I	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
Bsp1720I	GCTNAGC	1 cut(s)	1152
BspACI	CCGC	8 cut(s)	117, 229, 414, 985, 1034, 1501, 1669, 1831
BspANI	GGCC	3 cut(s)	426, 1490, 1698
BspDI	ATCGAT	1 cut(s)	1710
BspFNI	CGCG	2 cut(s)	231, 278
BspHI	TCATGA	2 cut(s)	849, 1615
BspLI	GGNNCC	2 cut(s)	166, 1002
BspMAI	CTGCAG	2 cut(s)	967, 1766
BspPI	GGATC	7 cut(s)	291, 464, 847, 854, 995, 1008, 1025
BspQI	GCTCTTC	1 cut(s)	210
BspTNI	GGTCTC	1 cut(s)	1630
BsrDI	GCAATG	2 cut(s)	302, 1699
BsrI	ACTGG	6 cut(s)	244, 295, 700, 836, 925, 1294
BssECI	CCNNGG	5 cut(s)	45, 622, 754, 805, 1205
BssMI	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
BssNI	GRCGYC	1 cut(s)	1296
BssT1I	CCWWGG	3 cut(s)	45, 754, 1205
Bst2UI	CCWGG	2 cut(s)	1005, 1524
Bst4CI	ACNGT	6 cut(s)	348, 420, 646, 1160, 1441, 1737
Bst6I	CTCTTC	4 cut(s)	78, 210, 1149, 1421
BstACI	GRCGYC	1 cut(s)	1296
BstC8I	GCNNGC	5 cut(s)	640, 657, 773, 936, 1095
BstDEI	CTNAG	4 cut(s)	1152, 1514, 1582, 1826
BstF5I	GGATG	3 cut(s)	618, 896, 1359
BstFNI	CGCG	2 cut(s)	231, 278
BstHHI	GCGC	2 cut(s)	712, 934
BstKTI	GATC	9 cut(s)	286, 472, 849, 855, 1003, 1033, 1174, 1195, 1552
BstMAI	GTCTC	2 cut(s)	457, 1630
BstMBI	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
BstMWI	GCNNNNNNNGC	7 cut(s)	716, 781, 865, 940, 1094, 1666, 1770
BstNI	CCWGG	2 cut(s)	1005, 1524
BstNSI	RCATGY	3 cut(s)	410, 1128, 1748
BstSCI	CCNGG	2 cut(s)	1003, 1522
BstSFI	CTRYAG	3 cut(s)	963, 988, 1762
BstUI	CGCG	2 cut(s)	231, 278
BstV1I	GCAGC	7 cut(s)	100, 502, 601, 706, 846, 1678, 1776
BstV2I	GAAGAC	1 cut(s)	1629
BstX2I	RGATCY	1 cut(s)	1000
BstXI	CCANNNNNNTGG	1 cut(s)	843
BstYI	RGATCY	1 cut(s)	1000
Bsu15I	ATCGAT	1 cut(s)	1710
BsuRI	GGCC	3 cut(s)	426, 1490, 1698
BsuTUI	ATCGAT	1 cut(s)	1710
BtgZI	GCGATG	1 cut(s)	1292
BtrI	CACGTC	1 cut(s)	478
BtsCI	GGATG	3 cut(s)	618, 896, 1359
BtsIMutI	CAGTG	3 cut(s)	215, 642, 1156
Cac8I	GCNNGC	5 cut(s)	640, 657, 773, 936, 1095
CciI	TCATGA	2 cut(s)	849, 1615
CfoI	GCGC	2 cut(s)	712, 934
Cfr13I	GGNCC	1 cut(s)	1520
ClaI	ATCGAT	1 cut(s)	1710
CseI	GACGC	4 cut(s)	220, 1285, 1440, 1634
Csp6I	GTAC	2 cut(s)	247, 1504
CviQI	GTAC	2 cut(s)	247, 1504
DdeI	CTNAG	4 cut(s)	1152, 1514, 1582, 1826
DpnI	GATC	9 cut(s)	285, 471, 848, 854, 1002, 1032, 1173, 1194, 1551
DpnII	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
EaeI	YGGCCR	3 cut(s)	424, 1488, 1696
Eam1104I	CTCTTC	4 cut(s)	78, 210, 1149, 1421
EarI	CTCTTC	4 cut(s)	78, 210, 1149, 1421
Eco130I	CCWWGG	3 cut(s)	45, 754, 1205
Eco31I	GGTCTC	1 cut(s)	1630
Eco47I	GGWCC	1 cut(s)	1520
Eco57I	CTGAAG	2 cut(s)	191, 1311
Eco88I	CYCGRG	1 cut(s)	1553
EcoRI	GAATTC	2 cut(s)	880, 1406
EcoRII	CCWGG	2 cut(s)	1003, 1522
EcoT14I	CCWWGG	3 cut(s)	45, 754, 1205
EcoT22I	ATGCAT	1 cut(s)	568
ErhI	CCWWGG	3 cut(s)	45, 754, 1205
FaqI	GGGAC	3 cut(s)	786, 840, 1830
FbaI	TGATCA	1 cut(s)	1192
FokI	GGATG	3 cut(s)	605, 883, 1366
FspBI	CTAG	4 cut(s)	560, 1274, 1368, 1400
FspI	TGCGCA	1 cut(s)	711
GlaI	GCGC	2 cut(s)	711, 933
GsaI	CCCAGC	2 cut(s)	947, 1101
GsuI	CTGGAG	1 cut(s)	819
HaeIII	GGCC	3 cut(s)	426, 1490, 1698
HgaI	GACGC	4 cut(s)	220, 1285, 1440, 1634
HhaI	GCGC	2 cut(s)	712, 934
Hin1I	GRCGYC	1 cut(s)	1296
Hin6I	GCGC	2 cut(s)	710, 932
HinP1I	GCGC	2 cut(s)	710, 932
HincII	GTYRAC	2 cut(s)	915, 1717
HindII	GTYRAC	2 cut(s)	915, 1717
HindIII	AAGCTT	1 cut(s)	137
HinfI	GANTC	9 cut(s)	40, 59, 536, 730, 828, 948, 1311, 1540, 1672
HpaI	GTTAAC	2 cut(s)	915, 1717
HphI	GGTGA	2 cut(s)	137, 1187
Hpy166II	GTNNAC	3 cut(s)	274, 915, 1717
Hpy188I	TCNGA	7 cut(s)	10, 178, 623, 809, 1016, 1139, 1576
Hpy188III	TCNNGA	5 cut(s)	473, 560, 850, 1315, 1616
Hpy8I	GTNNAC	3 cut(s)	274, 915, 1717
Hpy99I	CGWCG	2 cut(s)	236, 283
HpyAV	CCTTC	3 cut(s)	584, 991, 1335
HpyCH4III	ACNGT	6 cut(s)	348, 420, 646, 1160, 1441, 1737
HpyCH4IV	ACGT	2 cut(s)	447, 477
HpyF10VI	GCNNNNNNNGC	7 cut(s)	716, 781, 865, 940, 1094, 1666, 1770
HpyF3I	CTNAG	4 cut(s)	1152, 1514, 1582, 1826
HpySE526I	ACGT	2 cut(s)	447, 477
Hsp92I	GRCGYC	1 cut(s)	1296
HspAI	GCGC	2 cut(s)	710, 932
Ksp22I	TGATCA	1 cut(s)	1192
KspAI	GTTAAC	2 cut(s)	915, 1717
Kzo9I	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
LguI	GCTCTTC	1 cut(s)	210
LmnI	GCTCC	3 cut(s)	838, 1336, 1346
Lsp1109I	GCAGC	7 cut(s)	100, 502, 601, 706, 846, 1678, 1776
LweI	GCATC	7 cut(s)	175, 364, 553, 905, 1269, 1647, 1653
MaeI	CTAG	4 cut(s)	560, 1274, 1368, 1400
MaeII	ACGT	2 cut(s)	447, 477
MaeIII	GTNAC	7 cut(s)	443, 788, 1007, 1297, 1319, 1452, 1793
MalI	GATC	9 cut(s)	285, 471, 848, 854, 1002, 1032, 1173, 1194, 1551
MboI	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
MflI	RGATCY	1 cut(s)	1000
MlsI	TGGCCA	3 cut(s)	426, 1490, 1698
MluCI	AATT	8 cut(s)	628, 880, 1102, 1187, 1216, 1244, 1406, 1729
MluNI	TGGCCA	3 cut(s)	426, 1490, 1698
MlyI	GAGTC	2 cut(s)	822, 1320
MmeI	TCCRAC	1 cut(s)	1204
Mox20I	TGGCCA	3 cut(s)	426, 1490, 1698
Mph1103I	ATGCAT	1 cut(s)	568
MroXI	GAANNNNTTC	1 cut(s)	1248
MscI	TGGCCA	3 cut(s)	426, 1490, 1698
MseI	TTAA	4 cut(s)	914, 1064, 1436, 1716
MslI	CAYNNNNRTG	3 cut(s)	312, 1016, 1380
Msp20I	TGGCCA	3 cut(s)	426, 1490, 1698
MspA1I	CMGCKG	2 cut(s)	987, 1669
MspR9I	CCNGG	2 cut(s)	1005, 1524
Mva1269I	GAATGC	1 cut(s)	469
MvaI	CCWGG	2 cut(s)	1005, 1524
MvnI	CGCG	2 cut(s)	231, 278
MwoI	GCNNNNNNNGC	7 cut(s)	716, 781, 865, 940, 1094, 1666, 1770
NdeII	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
NlaIV	GGNNCC	2 cut(s)	166, 1002
NmuCI	GTSAC	6 cut(s)	443, 788, 1297, 1319, 1452, 1793
NsbI	TGCGCA	1 cut(s)	711
NsiI	ATGCAT	1 cut(s)	568
NspI	RCATGY	3 cut(s)	410, 1128, 1748
PagI	TCATGA	2 cut(s)	849, 1615
PciI	ACATGT	2 cut(s)	406, 1744
PciSI	GCTCTTC	1 cut(s)	210
PctI	GAATGC	1 cut(s)	469
PdmI	GAANNNNTTC	1 cut(s)	1248
PfeI	GAWTC	7 cut(s)	40, 59, 536, 730, 948, 1540, 1672
PflFI	GACNNNGTC	1 cut(s)	1821
PleI	GAGTC	2 cut(s)	822, 1319
PpsI	GAGTC	2 cut(s)	822, 1319
PscI	ACATGT	2 cut(s)	406, 1744
PsiI	TTATAA	1 cut(s)	1596
Psp6I	CCWGG	2 cut(s)	1003, 1522
PspFI	CCCAGC	2 cut(s)	943, 1097
PspGI	CCWGG	2 cut(s)	1003, 1522
PspN4I	GGNNCC	2 cut(s)	166, 1002
PspPI	GGNCC	1 cut(s)	1520
PsrI	GAACNNNNNNTAC	2 cut(s)	336, 368
PstI	CTGCAG	2 cut(s)	967, 1766
PsuI	RGATCY	1 cut(s)	1000
PsyI	GACNNNGTC	1 cut(s)	1821
RsaI	GTAC	2 cut(s)	248, 1505
RsaNI	GTAC	2 cut(s)	247, 1504
RseI	CAYNNNNRTG	3 cut(s)	312, 1016, 1380
SapI	GCTCTTC	1 cut(s)	210
SaqAI	TTAA	4 cut(s)	914, 1064, 1436, 1716
Sau3AI	GATC	9 cut(s)	283, 469, 846, 852, 1000, 1030, 1171, 1192, 1549
Sau96I	GGNCC	1 cut(s)	1520
SchI	GAGTC	2 cut(s)	822, 1320
ScrFI	CCNGG	2 cut(s)	1005, 1524
SfaNI	GCATC	7 cut(s)	175, 364, 553, 905, 1269, 1647, 1653
SfcI	CTRYAG	3 cut(s)	963, 988, 1762
SinI	GGWCC	1 cut(s)	1520
SmiMI	CAYNNNNRTG	3 cut(s)	312, 1016, 1380
SmlI	CTYRAG	1 cut(s)	1315
SmoI	CTYRAG	1 cut(s)	1315
Sse9I	AATT	8 cut(s)	628, 880, 1102, 1187, 1216, 1244, 1406, 1729
SsiI	CCGC	8 cut(s)	117, 229, 414, 985, 1034, 1501, 1669, 1831
SspMI	CTAG	4 cut(s)	560, 1274, 1368, 1400
StyD4I	CCNGG	2 cut(s)	1003, 1522
StyI	CCWWGG	3 cut(s)	45, 754, 1205
TaaI	ACNGT	6 cut(s)	348, 420, 646, 1160, 1441, 1737
TaiI	ACGT	2 cut(s)	450, 480
TaqI	TCGA	6 cut(s)	38, 62, 255, 766, 1147, 1710
TasI	AATT	8 cut(s)	628, 880, 1102, 1187, 1216, 1244, 1406, 1729
TauI	GCSGC	3 cut(s)	119, 416, 1833
TfiI	GAWTC	7 cut(s)	40, 59, 536, 730, 948, 1540, 1672
Tru1I	TTAA	4 cut(s)	914, 1064, 1436, 1716
Tru9I	TTAA	4 cut(s)	914, 1064, 1436, 1716
TscAI	CASTG	3 cut(s)	215, 649, 1163
TseFI	GTSAC	6 cut(s)	443, 788, 1297, 1319, 1452, 1793
TseI	GCWGC	7 cut(s)	113, 515, 614, 719, 859, 1666, 1764
Tsp45I	GTSAC	6 cut(s)	443, 788, 1297, 1319, 1452, 1793
TspDTI	ATGAA	8 cut(s)	131, 940, 1034, 1058, 1068, 1497, 1632, 1739
TspGWI	ACGGA	3 cut(s)	296, 395, 807
TspRI	CASTG	3 cut(s)	215, 649, 1163
Tth111I	GACNNNGTC	1 cut(s)	1821
VpaK11BI	GGWCC	1 cut(s)	1520
XapI	RAATTY	3 cut(s)	880, 1244, 1406
XbaI	TCTAGA	1 cut(s)	559
XceI	RCATGY	3 cut(s)	410, 1128, 1748
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1483
XmnI	GAANNNNTTC	1 cut(s)	1248
XspI	CTAG	4 cut(s)	560, 1274, 1368, 1400
Zsp2I	ATGCAT	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.