Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Reverse (-)

12239159 .. 12244112

Gene Structure 4954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1162500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGTCGTCGGAGATCGAGGTCGTCGAGGACGAGGTCCAAACCCGCGATCGCGAATCTGCCTCAGCCAATTCAGCAAACAACAATGGCGAAGCCAATGGGATTGGGGAAGAGAGTCTGCGAAACGACGTGTACACGGCAGCTGCGTACGGGGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGTTGCTCTGTTTCCGAGACCGATGGGCTCGGCTACTATGCCCTCCAGTGGGCCGCTCTCAACAACCGCACTGCCGCCGCTCAGTATATTATTGAGCACGGAGGAGATGTGAATGCCACAGATCATACGGGGCAGACAGCATTACACTGGAGTGCAGTGCGGGGTGCAATCCAGGTTGCAGAGCTATTACTTCAAGAGGGTGCTCGGGTAAATGCTGCAGATATGTATGGCTATCAGGCTACACATGTTGCGGCTCAATATGGTCAAACAGCTTTCCTGTACCATGTTGTTTCAAAATGGAATGCTGACCCTGATGTGCCTGATAATGACGGGAGAAGCCCTTTACACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTATTTCTGGATGCATATAAAGGACGCCAAGATAAAGAGGGTTGCACTCCTTTGCACTGGGCTGCTATCAGGGGTAACTTGGAGGCATGCACAGTGTTGGTACAGGCTGGAAAGAAGGAGGACTTAATGGTTACAGATAACACTGGCCTTACGCCAGCACAACTTGCTGCTGATAAGAATCACAGACAAGTTGCCTTTTTCCTTGGGAATGCTAGAAGATTGCTAGACAAACGCTGTGATGGGAACAGTCGTCTTGGAAGAATTTCGAAACTAGGACTTGCGCCACTACTTTGGTGTATAATATTTGTGCTCCTGGTGACCTATACTCATTCTGTCATTGCGGCACCAAATCTGCCAAAGTTAACAGCTGGATCAGTTTTTGTTGCATGGCTAGGAGTTTTCCTAGCAACATCCGGGCTTGTTTTGTTCTATAGATGCAGCAGCAAGGATCCAGGTTACATCCGAATGAATGTCCACGATTCACAAAATATGAAGGATGATGAGCCTCTTTTAAAGATTGAGATAAATAATCCTGCCTTGCTAGCTGGTAATTGGTCTCAGCTCTGTGCAACGTGCAAGATTGTCAGGCCTCTTCGTGCAAAGCACTGTTCAACCTGTGATCGTTGTGTTGAACAATTTGACCACCATTGCCCTTGGGTATCCAATTGCATTGGCAAGAAAAACAAATGGGATTTCTTTGCTTTCCTTTTTCTGGAAGTTTTGGCGATGGTGTTTACTGGAGGGGTTACTCTTACGAGAGTTTTGAGCGATCCATTGAGGCCATCTTCTTTTGGAGCATGGATCAATTATGTTGCTACTAGTCATGTGGGCGCTCTTTCATTTTTGATAGTGGATTTCCTCCTCTTCTTTGGCGTGGCAGTATTGACCGTTGTCCAAGCTTCTCAGATATCACGGAACATCACAACTAATGAAATGGCAAACATGATGCGCTATAACTACCTTAGAGGCCCAGGTGGTAGGTTCAGAAATCCGTACGATCACGGGATAAGGAAGAATTGTTCGGATTTCTTGATCAAGGGTTACAACGAAGATGTGGAATTCATTGAAGAATCAGCTCATGATGAGGAGGGTATTGGAATGAGGCATATCAATTCTTCAAACTTGCAAAATGGGGACGCCTATCCTCACCGCACAAACGGCAACAGTCATGTTGCAATCAATGTAAATTCTAATTCGACAGGACATCACGGTCACGTCCATTCTGCCCATTGCAGCCATAGCAATAGCCAGGGGAAACCGAAAACCGAAACGTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

619

Amino Acids

68.23

Weight (kDa)

6.29

Isoelectric Point (pI)

28.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	45 - 136	7.7e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	79 - 126	3.6e-07	Ankyrin repeats (many copies)
Ank_2	PF12796	144 - 230	9.6e-16	Ankyrin repeats (3 copies)
Ank_2	PF12796	199 - 260	7.8e-09	Ankyrin repeats (3 copies)
DHHC	PF01529	379 - 506	8.8e-32	DHHC palmitoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	546
AasI	GACNNNNNNGTC	1 cut(s)	1785
AccB1I	GGYRCC	1 cut(s)	919
AccBSI	CCGCTC	2 cut(s)	242, 266
AccI	GTMKAC	1 cut(s)	565
AccII	CGCG	2 cut(s)	45, 51
AciI	CCGC	9 cut(s)	43, 240, 253, 261, 264, 346, 437, 917, 1726
AclI	AACGTT	1 cut(s)	1847
AclWI	GGATC	5 cut(s)	955, 1019, 1032, 1340, 1385
AcsI	RAATTY	3 cut(s)	837, 1634, 1762
AcuI	CTGAAG	1 cut(s)	149
AcyI	GRCGYC	2 cut(s)	601, 1713
AfaI	GTAC	5 cut(s)	131, 146, 467, 678, 1571
AfiI	CCNNNNNNNGG	3 cut(s)	234, 235, 1288
AflIII	ACRYGT	2 cut(s)	126, 430
AgsI	TTSAA	6 cut(s)	380, 480, 1188, 1208, 1643, 1695
AhlI	ACTAGT	1 cut(s)	1394
AjiI	CACGTC	2 cut(s)	127, 1792
AjnI	CCWGG	5 cut(s)	357, 888, 1027, 1546, 1824
AleI	CACNNNNGTG	1 cut(s)	336
AloI	GAACNNNNNNTCC	2 cut(s)	1579, 1611
AluBI	AGCT	9 cut(s)	140, 163, 370, 458, 944, 1121, 1138, 1475, 1652
AluI	AGCT	9 cut(s)	140, 163, 370, 458, 944, 1121, 1138, 1475, 1652
Alw21I	GWGCWC	3 cut(s)	285, 391, 888
Alw26I	GTCTC	2 cut(s)	197, 1137
AlwI	GGATC	5 cut(s)	955, 1019, 1032, 1340, 1385
Ama87I	CYCGRG	1 cut(s)	390
AoxI	GGCC	5 cut(s)	237, 721, 1163, 1355, 1543
ApeKI	GCWGC	9 cut(s)	137, 140, 401, 539, 638, 743, 1014, 1017, 1809
ApoI	RAATTY	3 cut(s)	837, 1634, 1762
AsiSI	GCGATCGC	1 cut(s)	49
Asp700I	GAANNNNTTC	1 cut(s)	838
AspLEI	GCGC	3 cut(s)	859, 1409, 1527
AspS9I	GGNCC	3 cut(s)	34, 237, 1544
AsuC2I	CCSGG	1 cut(s)	991
AsuHPI	GGTGA	2 cut(s)	904, 1715
AsuII	TTCGAA	1 cut(s)	842
AsuNHI	GCTAGC	1 cut(s)	1117
AvaI	CYCGRG	1 cut(s)	390
AvaII	GGWCC	1 cut(s)	34
BamHI	GGATCC	1 cut(s)	1024
BanI	GGYRCC	1 cut(s)	919
BanII	GRGCYC	1 cut(s)	216
BbsI	GAAGAC	1 cut(s)	563
Bbv12I	GWGCWC	3 cut(s)	285, 391, 888
BbvCI	CCTCAGC	1 cut(s)	61
BbvI	GCAGC	9 cut(s)	127, 149, 388, 526, 625, 730, 1026, 1029, 1821
BccI	CCATC	4 cut(s)	203, 809, 1297, 1366
BceAI	ACGGC	2 cut(s)	150, 1750
BciT130I	CCWGG	5 cut(s)	359, 890, 1029, 1548, 1826
BciVI	GTATCC	1 cut(s)	1246
BclI	TGATCA	1 cut(s)	1608
BcnI	CCSGG	1 cut(s)	991
BcoDI	GTCTC	2 cut(s)	197, 1137
BcuI	ACTAGT	1 cut(s)	1394
BfaI	CTAG	7 cut(s)	789, 800, 848, 968, 980, 1118, 1395
BfmI	CTRYAG	2 cut(s)	402, 1006
BfoI	RGCGCY	1 cut(s)	1410
BfuI	GTATCC	1 cut(s)	1246
Bme1390I	CCNGG	6 cut(s)	359, 890, 991, 1029, 1548, 1826
Bme18I	GGWCC	1 cut(s)	34
BmeT110I	CYCGRG	1 cut(s)	390
BmgBI	CACGTC	2 cut(s)	127, 1792
BmgT120I	GGNCC	3 cut(s)	34, 237, 1544
BmiI	GGNNCC	2 cut(s)	921, 1026
BmrFI	CCNGG	6 cut(s)	359, 890, 991, 1029, 1548, 1826
BmrI	ACTGGG	2 cut(s)	544, 643
BmsI	GCATC	3 cut(s)	577, 1001, 1512
BmtI	GCTAGC	1 cut(s)	1121
BmuI	ACTGGG	2 cut(s)	544, 643
BoxI	GACNNNNGTC	1 cut(s)	1466
BpiI	GAAGAC	1 cut(s)	563
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	3 cut(s)	215, 355, 1335
Bpu10I	CCTNAGC	1 cut(s)	61
Bpu14I	TTCGAA	1 cut(s)	842
BpuMI	CCSGG	1 cut(s)	991
BsaBI	GATNNNNATC	1 cut(s)	753
BsaHI	GRCGYC	2 cut(s)	601, 1713
BsaI	GGTCTC	2 cut(s)	197, 1137
BsaJI	CCNNGG	4 cut(s)	778, 1229, 1546, 1825
BsaXI	ACNNNNNCTCC	2 cut(s)	279, 309
Bsc4I	CCNNNNNNNGG	3 cut(s)	234, 235, 1288
Bse1I	ACTGG	6 cut(s)	232, 338, 539, 638, 724, 1318
Bse3DI	GCAATG	3 cut(s)	912, 1222, 1804
Bse8I	GATNNNNATC	1 cut(s)	753
BseBI	CCWGG	5 cut(s)	359, 890, 1029, 1548, 1826
BseDI	CCNNGG	4 cut(s)	778, 1229, 1546, 1825
BseGI	GGATG	4 cut(s)	592, 986, 1035, 1078
BseJI	GATNNNNATC	1 cut(s)	753
BseLI	CCNNNNNNNGG	3 cut(s)	234, 235, 1288
BseMI	GCAATG	3 cut(s)	912, 1222, 1804
BseMII	CTCAG	4 cut(s)	75, 281, 1148, 1493
BseNI	ACTGG	6 cut(s)	232, 338, 539, 638, 724, 1318
BseRI	GAGGAG	3 cut(s)	303, 1427, 1676
BseXI	GCAGC	9 cut(s)	127, 149, 388, 526, 625, 730, 1026, 1029, 1821
BsgI	GTGCAG	1 cut(s)	360
Bsh1236I	CGCG	2 cut(s)	45, 51
Bsh1285I	CGRYCG	1 cut(s)	49
BshFI	GGCC	5 cut(s)	239, 723, 1165, 1357, 1545
BshNI	GGYRCC	1 cut(s)	919
BsiEI	CGRYCG	1 cut(s)	49
BsiHKAI	GWGCWC	3 cut(s)	285, 391, 888
BsiHKCI	CYCGRG	1 cut(s)	390
BsiSI	CCGG	1 cut(s)	990
BsiWI	CGTACG	2 cut(s)	144, 1569
BslFI	GGGAC	1 cut(s)	1724
BslI	CCNNNNNNNGG	3 cut(s)	234, 235, 1288
BsmAI	GTCTC	2 cut(s)	197, 1137
BsmFI	GGGAC	1 cut(s)	1724
BsmI	GAATGC	3 cut(s)	304, 493, 790
BsnI	GGCC	5 cut(s)	239, 723, 1165, 1357, 1545
Bso31I	GGTCTC	2 cut(s)	197, 1137
BsoBI	CYCGRG	1 cut(s)	390
Bsp119I	TTCGAA	1 cut(s)	842
Bsp1286I	GDGCHC	4 cut(s)	216, 285, 391, 888
Bsp1407I	TGTACA	1 cut(s)	129
Bsp68I	TCGCGA	1 cut(s)	51
BspACI	CCGC	9 cut(s)	43, 240, 253, 261, 264, 346, 437, 917, 1726
BspANI	GGCC	5 cut(s)	239, 723, 1165, 1357, 1545
BspCNI	CTCAG	4 cut(s)	74, 280, 1147, 1492
BspFNI	CGCG	2 cut(s)	45, 51
BspHI	TCATGA	1 cut(s)	1654
BspLI	GGNNCC	2 cut(s)	921, 1026
BspMAI	CTGCAG	1 cut(s)	406
BspOI	GCTAGC	1 cut(s)	1121
BspPI	GGATC	5 cut(s)	955, 1019, 1032, 1340, 1385
BspT104I	TTCGAA	1 cut(s)	842
BspT107I	GGYRCC	1 cut(s)	919
BspTNI	GGTCTC	2 cut(s)	197, 1137
BsrBI	CCGCTC	2 cut(s)	242, 266
BsrDI	GCAATG	3 cut(s)	912, 1222, 1804
BsrGI	TGTACA	1 cut(s)	129
BsrI	ACTGG	6 cut(s)	232, 338, 539, 638, 724, 1318
BssECI	CCNNGG	4 cut(s)	778, 1229, 1546, 1825
BssNAI	GTATAC	1 cut(s)	566
BssNI	GRCGYC	2 cut(s)	601, 1713
BssT1I	CCWWGG	2 cut(s)	778, 1229
Bst1107I	GTATAC	1 cut(s)	566
Bst2UI	CCWGG	5 cut(s)	359, 890, 1029, 1548, 1826
Bst4CI	ACNGT	6 cut(s)	670, 824, 1184, 1465, 1742, 1787
Bst6I	CTCTTC	3 cut(s)	102, 1173, 1445
BstACI	GRCGYC	2 cut(s)	601, 1713
BstAPI	GCANNNNNTGC	1 cut(s)	740
BstAUI	TGTACA	1 cut(s)	129
BstBI	TTCGAA	1 cut(s)	842
BstC8I	GCNNGC	3 cut(s)	664, 732, 1119
BstDEI	CTNAG	5 cut(s)	61, 267, 1134, 1479, 1538
BstEII	GGTNACC	1 cut(s)	892
BstF5I	GGATG	4 cut(s)	592, 986, 1035, 1078
BstFNI	CGCG	2 cut(s)	45, 51
BstH2I	RGCGCY	1 cut(s)	1410
BstHHI	GCGC	3 cut(s)	859, 1409, 1527
BstMAI	GTCTC	2 cut(s)	197, 1137
BstMCI	CGRYCG	1 cut(s)	49
BstMWI	GCNNNNNNNGC	5 cut(s)	71, 740, 1118, 1734, 1815
BstNI	CCWGG	5 cut(s)	359, 890, 1029, 1548, 1826
BstNSI	RCATGY	2 cut(s)	434, 666
BstPAI	GACNNNNGTC	1 cut(s)	1466
BstPI	GGTNACC	1 cut(s)	892
BstSCI	CCNGG	6 cut(s)	357, 888, 989, 1027, 1546, 1824
BstSFI	CTRYAG	2 cut(s)	402, 1006
BstUI	CGCG	2 cut(s)	45, 51
BstV1I	GCAGC	9 cut(s)	127, 149, 388, 526, 625, 730, 1026, 1029, 1821
BstV2I	GAAGAC	1 cut(s)	563
BstX2I	RGATCY	1 cut(s)	1024
BstXI	CCANNNNNNTGG	1 cut(s)	867
BstYI	RGATCY	1 cut(s)	1024
BstZ17I	GTATAC	1 cut(s)	566
BsuI	GTATCC	1 cut(s)	1246
BsuRI	GGCC	5 cut(s)	239, 723, 1165, 1357, 1545
BtgZI	GCGATG	1 cut(s)	1316
BtrI	CACGTC	2 cut(s)	127, 1792
BtsCI	GGATG	4 cut(s)	592, 986, 1035, 1078
BtsI	GCAGTG	2 cut(s)	255, 348
BtsIMutI	CAGTG	9 cut(s)	239, 255, 331, 348, 532, 631, 675, 717, 1180
BtuMI	TCGCGA	1 cut(s)	51
Cac8I	GCNNGC	3 cut(s)	664, 732, 1119
CciI	TCATGA	1 cut(s)	1654
CfoI	GCGC	3 cut(s)	859, 1409, 1527
Cfr13I	GGNCC	3 cut(s)	34, 237, 1544
CseI	GACGC	2 cut(s)	609, 1721
Csp6I	GTAC	5 cut(s)	130, 145, 466, 677, 1570
CviAII	CATG	9 cut(s)	431, 470, 663, 963, 1374, 1400, 1519, 1655, 1745
CviQI	GTAC	5 cut(s)	130, 145, 466, 677, 1570
DdeI	CTNAG	5 cut(s)	61, 267, 1134, 1479, 1538
DraI	TTTAAA	1 cut(s)	1089
DrdI	GACNNNNNNGTC	1 cut(s)	1785
DseDI	GACNNNNNNGTC	1 cut(s)	1785
Eam1104I	CTCTTC	3 cut(s)	102, 1173, 1445
EarI	CTCTTC	3 cut(s)	102, 1173, 1445
Eco130I	CCWWGG	2 cut(s)	778, 1229
Eco147I	AGGCCT	1 cut(s)	1165
Eco24I	GRGCYC	1 cut(s)	216
Eco31I	GGTCTC	2 cut(s)	197, 1137
Eco32I	GATATC	1 cut(s)	1485
Eco47I	GGWCC	1 cut(s)	34
Eco57I	CTGAAG	1 cut(s)	149
Eco88I	CYCGRG	1 cut(s)	390
Eco91I	GGTNACC	1 cut(s)	892
EcoO65I	GGTNACC	1 cut(s)	892
EcoRI	GAATTC	1 cut(s)	1634
EcoRII	CCWGG	5 cut(s)	357, 888, 1027, 1546, 1824
EcoRV	GATATC	1 cut(s)	1485
EcoT14I	CCWWGG	2 cut(s)	778, 1229
EcoT22I	ATGCAT	1 cut(s)	592
EcoT38I	GRGCYC	1 cut(s)	216
ErhI	CCWWGG	2 cut(s)	778, 1229
FaeI	CATG	9 cut(s)	434, 473, 666, 966, 1377, 1403, 1522, 1658, 1748
FalI	AAGNNNNNCTT	2 cut(s)	683, 715
FaqI	GGGAC	1 cut(s)	1724
FatI	CATG	9 cut(s)	430, 469, 662, 962, 1373, 1399, 1518, 1654, 1744
FauI	CCCGC	2 cut(s)	50, 339
FbaI	TGATCA	1 cut(s)	1608
FblI	GTMKAC	1 cut(s)	565
FokI	GGATG	4 cut(s)	599, 973, 1022, 1085
FriOI	GRGCYC	1 cut(s)	216
FspBI	CTAG	7 cut(s)	789, 800, 848, 968, 980, 1118, 1395
GlaI	GCGC	3 cut(s)	858, 1408, 1526
GsuI	CTGGAG	3 cut(s)	215, 355, 1335
HaeII	RGCGCY	1 cut(s)	1410
HaeIII	GGCC	5 cut(s)	239, 723, 1165, 1357, 1545
HapII	CCGG	1 cut(s)	990
HgaI	GACGC	2 cut(s)	609, 1721
HhaI	GCGC	3 cut(s)	859, 1409, 1527
Hin1I	GRCGYC	2 cut(s)	601, 1713
Hin1II	CATG	9 cut(s)	434, 473, 666, 966, 1377, 1403, 1522, 1658, 1748
Hin6I	GCGC	3 cut(s)	857, 1407, 1525
HinP1I	GCGC	3 cut(s)	857, 1407, 1525
HincII	GTYRAC	1 cut(s)	939
HindII	GTYRAC	1 cut(s)	939
HindIII	AAGCTT	2 cut(s)	161, 1473
HinfI	GANTC	5 cut(s)	53, 112, 754, 1055, 1646
HpaI	GTTAAC	1 cut(s)	939
HpaII	CCGG	1 cut(s)	990
HphI	GGTGA	2 cut(s)	904, 1715
Hpy166II	GTNNAC	6 cut(s)	130, 132, 566, 939, 1051, 1311
Hpy188I	TCNGA	7 cut(s)	10, 168, 202, 1040, 1482, 1562, 1600
Hpy188III	TCNNGA	6 cut(s)	50, 380, 584, 1289, 1606, 1655
Hpy8I	GTNNAC	6 cut(s)	130, 132, 566, 939, 1051, 1311
Hpy99I	CGWCG	3 cut(s)	10, 26, 128
HpyAV	CCTTC	2 cut(s)	685, 1063
HpyCH4III	ACNGT	6 cut(s)	670, 824, 1184, 1465, 1742, 1787
HpyCH4IV	ACGT	5 cut(s)	126, 568, 1148, 1791, 1847
HpyF10VI	GCNNNNNNNGC	5 cut(s)	71, 740, 1118, 1734, 1815
HpyF3I	CTNAG	5 cut(s)	61, 267, 1134, 1479, 1538
HpySE526I	ACGT	5 cut(s)	126, 568, 1148, 1791, 1847
Hsp92I	GRCGYC	2 cut(s)	601, 1713
Hsp92II	CATG	9 cut(s)	434, 473, 666, 966, 1377, 1403, 1522, 1658, 1748
HspAI	GCGC	3 cut(s)	857, 1407, 1525
Ksp22I	TGATCA	1 cut(s)	1608
KspAI	GTTAAC	1 cut(s)	939
LmnI	GCTCC	2 cut(s)	891, 1370
Lsp1109I	GCAGC	9 cut(s)	127, 149, 388, 526, 625, 730, 1026, 1029, 1821
LweI	GCATC	3 cut(s)	577, 1001, 1512
MaeI	CTAG	7 cut(s)	789, 800, 848, 968, 980, 1118, 1395
MaeII	ACGT	5 cut(s)	126, 568, 1148, 1791, 1847
MaeIII	GTNAC	7 cut(s)	650, 706, 892, 1031, 1321, 1616, 1787
MbiI	CCGCTC	2 cut(s)	242, 266
MfeI	CAATTG	1 cut(s)	1240
MflI	RGATCY	1 cut(s)	1024
MhlI	GDGCHC	4 cut(s)	216, 285, 391, 888
MlyI	GAGTC	1 cut(s)	121
Mph1103I	ATGCAT	1 cut(s)	592
MroXI	GAANNNNTTC	1 cut(s)	838
MseI	TTAA	3 cut(s)	701, 938, 1088
MslI	CAYNNNNRTG	2 cut(s)	336, 1040
MspA1I	CMGCKG	2 cut(s)	140, 944
MspI	CCGG	1 cut(s)	990
MspR9I	CCNGG	6 cut(s)	359, 890, 991, 1029, 1548, 1826
MunI	CAATTG	1 cut(s)	1240
Mva1269I	GAATGC	3 cut(s)	304, 493, 790
MvaI	CCWGG	5 cut(s)	359, 890, 1029, 1548, 1826
MvnI	CGCG	2 cut(s)	45, 51
MwoI	GCNNNNNNNGC	5 cut(s)	71, 740, 1118, 1734, 1815
NciI	CCSGG	1 cut(s)	991
NheI	GCTAGC	1 cut(s)	1117
NlaIII	CATG	9 cut(s)	434, 473, 666, 966, 1377, 1403, 1522, 1658, 1748
NlaIV	GGNNCC	2 cut(s)	921, 1026
NmeAIII	GCCGAG	1 cut(s)	195
NmuCI	GTSAC	2 cut(s)	892, 1787
NruI	TCGCGA	1 cut(s)	51
NsiI	ATGCAT	1 cut(s)	592
NspI	RCATGY	2 cut(s)	434, 666
NspV	TTCGAA	1 cut(s)	842
OliI	CACNNNNGTG	1 cut(s)	336
PaeI	GCATGC	1 cut(s)	666
PagI	TCATGA	1 cut(s)	1654
PceI	AGGCCT	1 cut(s)	1165
PciI	ACATGT	1 cut(s)	430
PcsI	WCGNNNNNNNCGW	3 cut(s)	21, 27, 140
PctI	GAATGC	3 cut(s)	304, 493, 790
PdmI	GAANNNNTTC	1 cut(s)	838
PfeI	GAWTC	4 cut(s)	53, 754, 1055, 1646
Pfl23II	CGTACG	2 cut(s)	144, 1569
PflFI	GACNNNGTC	1 cut(s)	32
Ple19I	CGATCG	1 cut(s)	49
PleI	GAGTC	1 cut(s)	120
PpsI	GAGTC	1 cut(s)	120
PscI	ACATGT	1 cut(s)	430
PshAI	GACNNNNGTC	1 cut(s)	1466
PsiI	TTATAA	1 cut(s)	546
Psp1406I	AACGTT	1 cut(s)	1847
Psp6I	CCWGG	5 cut(s)	357, 888, 1027, 1546, 1824
PspEI	GGTNACC	1 cut(s)	892
PspGI	CCWGG	5 cut(s)	357, 888, 1027, 1546, 1824
PspLI	CGTACG	2 cut(s)	144, 1569
PspN4I	GGNNCC	2 cut(s)	921, 1026
PspPI	GGNCC	3 cut(s)	34, 237, 1544
PstI	CTGCAG	1 cut(s)	406
PsuI	RGATCY	1 cut(s)	1024
PsyI	GACNNNGTC	1 cut(s)	32
PvuI	CGATCG	1 cut(s)	49
PvuII	CAGCTG	2 cut(s)	140, 944
RgaI	GCGATCGC	1 cut(s)	49
RruI	TCGCGA	1 cut(s)	51
RsaI	GTAC	5 cut(s)	131, 146, 467, 678, 1571
RsaNI	GTAC	5 cut(s)	130, 145, 466, 677, 1570
RseI	CAYNNNNRTG	2 cut(s)	336, 1040
SaqAI	TTAA	3 cut(s)	701, 938, 1088
Sau96I	GGNCC	3 cut(s)	34, 237, 1544
SchI	GAGTC	1 cut(s)	121
ScrFI	CCNGG	6 cut(s)	359, 890, 991, 1029, 1548, 1826
SduI	GDGCHC	4 cut(s)	216, 285, 391, 888
SfaAI	GCGATCGC	1 cut(s)	49
SfaNI	GCATC	3 cut(s)	577, 1001, 1512
SfcI	CTRYAG	2 cut(s)	402, 1006
SfuI	TTCGAA	1 cut(s)	842
SgfI	GCGATCGC	1 cut(s)	49
SinI	GGWCC	1 cut(s)	34
SmiMI	CAYNNNNRTG	2 cut(s)	336, 1040
SpeI	ACTAGT	1 cut(s)	1394
SphI	GCATGC	1 cut(s)	666
SseBI	AGGCCT	1 cut(s)	1165
SsiI	CCGC	9 cut(s)	43, 240, 253, 261, 264, 346, 437, 917, 1726
SspI	AATATT	1 cut(s)	879
SspMI	CTAG	7 cut(s)	789, 800, 848, 968, 980, 1118, 1395
StuI	AGGCCT	1 cut(s)	1165
StyD4I	CCNGG	6 cut(s)	357, 888, 989, 1027, 1546, 1824
StyI	CCWWGG	2 cut(s)	778, 1229
TaaI	ACNGT	6 cut(s)	670, 824, 1184, 1465, 1742, 1787
TaiI	ACGT	5 cut(s)	129, 571, 1151, 1794, 1850
TaqI	TCGA	4 cut(s)	15, 24, 842, 1772
TaqII	GACCGA	1 cut(s)	221
TatI	WGTACW	1 cut(s)	129
TauI	GCSGC	5 cut(s)	242, 263, 266, 440, 920
TfiI	GAWTC	4 cut(s)	53, 754, 1055, 1646
Tru1I	TTAA	3 cut(s)	701, 938, 1088
Tru9I	TTAA	3 cut(s)	701, 938, 1088
TscAI	CASTG	9 cut(s)	239, 262, 338, 348, 539, 638, 675, 724, 1187
TseFI	GTSAC	2 cut(s)	892, 1787
TseI	GCWGC	9 cut(s)	137, 140, 401, 539, 638, 743, 1014, 1017, 1809
Tsp45I	GTSAC	2 cut(s)	892, 1787
TspDTI	ATGAA	5 cut(s)	1058, 1082, 1404, 1521, 1627
TspGWI	ACGGA	3 cut(s)	300, 1504, 1557
TspRI	CASTG	9 cut(s)	239, 262, 338, 348, 539, 638, 675, 724, 1187
Tth111I	GACNNNGTC	1 cut(s)	32
VpaK11BI	GGWCC	1 cut(s)	34
XapI	RAATTY	3 cut(s)	837, 1634, 1762
XceI	RCATGY	2 cut(s)	434, 666
XmiI	GTMKAC	1 cut(s)	565
XmnI	GAANNNNTTC	1 cut(s)	838
XspI	CTAG	7 cut(s)	789, 800, 848, 968, 980, 1118, 1395
Zsp2I	ATGCAT	1 cut(s)	592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.