Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Forward (+)

90614642 .. 90628096

Gene Structure 13455 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00153890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3708 bp

ATGTCGTCGGAGATCGAGGTCGTCGAGGAGGACCAGGGCCAAAACCGCAGCGATCACGAATCGGCGGCGGTAGCCGGTTCCGTCAATTCGGGCGGTGGAGAAGCCAATGGGATTGGGGAGGAGAGTCTGCGAAACGACGTGTACACGGCGGCGGCCTACGGCGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGTTGCTCGGTTTCCGAGACCGATGGGCTGGGCTACTACGCGCTCCAGTGGGCCGCCCTTAACAACCGAACTGCCGCCGCCCAGTATATTATAGAGCATGGAGGAGATGTGAATGCGAAGGACCATGCGGGGCAGACGGCATTGCACTGGAGTGCAGTGCGGGGTGCTATCCAGGTTGCGGAGCTATTACTCCAAGAGGGTGCGAGGGTGAATGCGGGTGATATGTATGGCTATCAGACTACACATGTTGCGGCCCAATATGGTCAGACTGCTTTCCTATACCATATTGTTTCAAAATGGAATGCTGATCCTGATGTTCCTGATAATGATGGAAGAAGCCCTTTGCACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTGTTTCTGGATGCATATAGAGGGCGCCAAGATAAAGAGGGTTGCACTCCTCTACATTGGGCTGCTATCAGGGGTAACTTGGAGGCATGCACAGTTTTGGTACAAGCTGGGAAAAAGGAGGACTTGATGGTTACAGATAACACTGGCCTTACACCAGCACAACTTGCTTCTGATAAAAACCACAGACAAGTTGCTTTTTTCCTTGGGAATGCTCGAAGGCTGCTTGACAAACGTTGTGATGGGAACAGTCGTCTTGGACAACTTTCAAAACTAGGACTTGCACCAGGACTTTGGTGTATAATCTTTGTGCTCCTTGTGACCTATACTCATTCTGTTATCATGGCACCAAATCTGCCAAAGTTAACAGCTGGCTCAGTTTTTATTGCATGGCTTGGAGTTTTCCTTGCAACTGCAGGGCTGGTTATGTTATATCGGTGTAGCAGTAAAGATCCCGGTTACATCCGAATGAATATCCATGATTCACAAAATATGAAAGATGATGAACCTCTTTTGAAGATTGAGATAAATAATCCGGCCTTGCTGGCTGGTAACTGGTCTCAGCTCTGTGCAACTTGCAAGATTGTCAGGCCTCTTCGTGCAAAGCACTGTTCGACTTGTGACCGTTGTGTTGAACAATTTGACCACCATTGCCCTTGGGTATCCAATTGCATTGGCAAGAAAAACAAATGGGATTTCTTTATTTTCCTTGTTCTTGAAGTTTTGGCAATGTTGACTACTGGAGGAGTCACTCTTACGAGAATTTGGAGTGATCCGCTCGGTCCATCTTCATTTGGAGCATGGCTTAATCATGTTGGTACAAGTCATATTGGTGCTATATCATTTTTTATTGTTGATTTCTTCCTCTTCTTCGGTGTGGCCGTTTTGACTGTTGTGCAAGTTTCTCAAATTGCGCGAAATATTACTACTAATGAAATGGCAAATGCAATGCGCTATAACTACCTTAGAGGGCCAGGTGGTCGCTTCAGAAACCCATATGATCATGGGATGAAGAAGAATTGCTCAGATTTCTTAATCAAGGGCTACAATGAAGATGTGGAATTGATTGAAGAATCAACTCGTGCTGAGGGGACTGGTATGAGGCACATGAATGGGGATTCCCATTCTCACCACACAAATGGCAATGGTCATGTTGCAATCAACGTAAATTCGAATGCATCAGCACATCAAGGTCATGTTCATTCTGCCCATTGCAGCCATAACAATCATGGTAAACCAAAAACTGATAATGTCCCCGTAGGCTTGGGTCTTGGCCTTGGACGGAACGCTTCCCGACCTACTGTGTTTAATCTCTTTAATGATGTTGAGGGTGATATTGATCATATATTCATGTTAGTGTGGTGTGGAGTGGAGTGGAGTTCACGGCACATGAAGGCCTATTCTGGAGCTAGAATGGGCTGCAATAGAGACCAACCGGTCATTGAAGAAGGCGGTGTTAGTAGAGTAGTGTCTGGAAGATATTGTAAAAGTAGCAGTTCAAAATGGCTAGGAACTTCATCTTTTCGTTCCACTGCAGAACAGTCTCCAGGAGTAGCAGGCATTTGCCCATCTCTCTTGGAATTATGCATTTCCAAAATATGCCGGGACATTGACAAATACAGTTCGCTTTCCCTGCTGCCTAGGGATGTGAGTCAGCAAATCTTTAATGAATTGGTCTGTTCCCAATCTCTCACTCATGATTCTCTCCAGGCTTTTAGAGATTGTGCTCTTGAGGATTTTGGTTTGGGCGAATACCCTTCTGTGAATGATAGTTGGATGGATGCCATCTGTTCACAAGGTTCATCTCTACTATCTGTTGATCTTTCTGGTTCCGAGGTGACAGATGCTGGATTGGCTCTTCTAAAAGACTGCTCGAACCTCCAATCATTAACTTATAATTACTGTGACCATGTTTCAGAACATGGACTTAAGCACATCAGCGGTCTTTCAAATTTGAAATGCTTGAGTTTTAAAAGGAGCAGTGCAATTAGTGCTGAAGGGATGCACGCCTTCTCCAGCCTTTTTAACTTAGAAAAGTTGGACTTGGAGAGGTGTCCAGAGATTCATGGTGGCTTTGTTCATCTTAAAGGTTTGACGAAACTAAAGTCTCTTAATGTTAGATGTTGTAAATGCGTCACAGATTCAGATTTAAAGGCCATCTCAGGCTTGCAGAAGCTTAATATGTTGAACTTAGAGGGATGCAATGTTACTTCTGCATGTTTGGAATCTATTTCAGCTCTTGTTGCCCTGGCTTACTTAAATCTCAACAGATGCAGTTTATCTGATGAAGGATGTGATAAGTTTTCTGGCCTTACAAACTTGAAGGTTTTGAGCTTGGGATTCAACAGTATCACAGATGCATGTTTGGTGCATCTAAAAGGTTTGACAAATTTGGAGAGCTTGAACCTGGATTCTTGTAAGATAGGTGACGAGGGGCTTGCAAACTTGGCAGGGTTTGTACTCTTGAAGAATTTGGAGTTGTCTGATACTGAAGTTGGAAGCAATGGGATTCGTCATCTCTCAGGGTTAAAGAATCTGGAGAAATTAAACTTGTCGTTCACCCTGGTAACTGACAGTAGTCTGAAAAAGTTATCTGGACTGACATCTCTCACATCACTTAATCTGGATGCTCGCCAAATTACTGATGCTGGACTTGCAGCTATTACAGGTCTTACAAAATTGACGCATCTGGATCTCTTTGGCGCTCGGATTTCAGACTCTGGAGCAAACTCTTTAAAACACTTCAAGAACCTTCAATCTCTTGAAATATGTGGTGGAGGATTGACTGATGCTGGTGTGAAGAATCTCAAAGATCTTGTCTGTCTGACATGGCTAAATTTGTCGCAGAACTGCAACTTGACTGATAAATCCTTGGAAGTGATTTCTGGTTTGACTGCATTGGTGTCCTTAAACATTTCAAATTCACGCATTACCAATGAGGGATTGCAGTATTTAAAGCCTTTGAAGAATCTACGTTCCTTAACCTTGGAGTCCAGTAAGGTGACTGCATCTGAAATCAGGAAGCTACAGTTTGCTGCCCTCCCTAATCTTCACAATGATGTTGGGAAATTTTATAGTGCAGTTGGTGTATTGATGCTTCATGTTACGCATTATTATCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

1235

Amino Acids

134.42

Weight (kDa)

6.35

Isoelectric Point (pI)

33.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	49 - 139	5.6e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	83 - 130	1.6e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	148 - 234	8.5e-16	Ankyrin repeats (3 copies)
Ank_2	PF12796	205 - 264	1.1e-07	Ankyrin repeats (3 copies)
DHHC	PF01529	383 - 510	3.1e-32	DHHC palmitoyltransferase
LRR_14	PF23598	896 - 1069	6.5e-08	Leucine-rich repeat region
LRR_8	PF13855	945 - 1003	2.4e-07	Leucine rich repeat
LRR_14	PF23598	1057 - 1210	3.4e-07	Leucine-rich repeat region
LRR_8	PF13855	1112 - 1172	3.2e-07	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	558, 2481
AasI	GACNNNNNNGTC	1 cut(s)	2021
AccB1I	GGYRCC	2 cut(s)	612, 931
AccBSI	CCGCTC	1 cut(s)	1363
AccI	GTMKAC	1 cut(s)	577
AccII	CGCG	2 cut(s)	239, 1501
AclI	AACGTT	1 cut(s)	820
AclWI	GGATC	4 cut(s)	500, 1031, 1352, 3285
AcoI	YGGCCR	1 cut(s)	1464
AcsI	RAATTY	8 cut(s)	1347, 1753, 2536, 2974, 3055, 3421, 3505, 3653
AcuI	CTGAAG	4 cut(s)	161, 1555, 2601, 3096
AcyI	GRCGYC	1 cut(s)	613
AfaI	GTAC	4 cut(s)	143, 690, 1405, 3045
AfiI	CCNNNNNNNGG	1 cut(s)	376
AflII	CTTAAG	1 cut(s)	2513
AflIII	ACRYGT	2 cut(s)	138, 442
AgeI	ACCGGT	1 cut(s)	2020
AjiI	CACGTC	1 cut(s)	139
AjnI	CCWGG	9 cut(s)	33, 369, 871, 1558, 2131, 2292, 2832, 2991, 3147
AleI	CACNNNNGTG	1 cut(s)	348
Alw21I	GWGCWC	2 cut(s)	900, 2314
Alw26I	GTCTC	5 cut(s)	209, 1149, 2007, 2133, 2697
AlwI	GGATC	4 cut(s)	500, 1031, 1352, 3285
AlwNI	CAGNNNCTG	2 cut(s)	2432, 3305
ApeKI	GCWGC	9 cut(s)	48, 551, 650, 808, 1800, 2004, 2221, 3242, 3620
ApoI	RAATTY	8 cut(s)	1347, 1753, 2536, 2974, 3055, 3421, 3505, 3653
ArsI	GACNNNNNNTTYG	4 cut(s)	2675, 2707, 3387, 3419
AsiGI	ACCGGT	1 cut(s)	2020
AspA2I	CCTAGG	1 cut(s)	2225
AspLEI	GCGC	5 cut(s)	241, 615, 1501, 1539, 3290
AspS9I	GGNCC	7 cut(s)	31, 37, 249, 319, 451, 1367, 1556
AsuC2I	CCSGG	2 cut(s)	1041, 2189
AsuHPI	GGTGA	8 cut(s)	418, 428, 1706, 1928, 2434, 3023, 3136, 3598
AsuII	TTCGAA	1 cut(s)	1757
AvaII	GGWCC	3 cut(s)	31, 319, 1367
AvrII	CCTAGG	1 cut(s)	2225
BaeI	ACNNNNGTAYC	2 cut(s)	3063, 3096
BanI	GGYRCC	2 cut(s)	612, 931
BarI	GAAGNNNNNNTAC	2 cut(s)	3666, 3698
BauI	CACGAG	1 cut(s)	1665
BbsI	GAAGAC	1 cut(s)	575
Bbv12I	GWGCWC	2 cut(s)	900, 2314
BbvCI	CCTCAGC	1 cut(s)	1671
BbvI	GCAGC	9 cut(s)	60, 538, 637, 795, 1812, 1991, 2208, 3254, 3607
BccI	CCATC	9 cut(s)	215, 521, 709, 821, 1378, 2161, 2356, 2378, 2750
BceAI	ACGGC	5 cut(s)	162, 175, 351, 1451, 1985
BcgI	CGANNNNNNTGC	2 cut(s)	3005, 3039
BciT130I	CCWGG	9 cut(s)	35, 371, 873, 1560, 2133, 2294, 2834, 2993, 3149
BciVI	GTATCC	1 cut(s)	1258
BclI	TGATCA	2 cut(s)	1585, 1924
BcnI	CCSGG	2 cut(s)	1041, 2189
BcoDI	GTCTC	5 cut(s)	209, 1149, 2007, 2133, 2697
BfaI	CTAG	4 cut(s)	860, 1995, 2093, 2226
BfmI	CTRYAG	4 cut(s)	999, 2118, 3611, 3704
BfoI	RGCGCY	2 cut(s)	616, 3291
BfrI	CTTAAG	1 cut(s)	2513
BfuI	GTATCC	1 cut(s)	1258
BglI	GCCNNNNNGGC	1 cut(s)	1130
BglII	AGATCT	1 cut(s)	3397
BlnI	CCTAGG	1 cut(s)	2225
Bme18I	GGWCC	3 cut(s)	31, 319, 1367
BmgBI	CACGTC	1 cut(s)	139
BmgT120I	GGNCC	7 cut(s)	31, 37, 249, 319, 451, 1367, 1556
BmiI	GGNNCC	4 cut(s)	79, 614, 933, 2416
BmrI	ACTGGG	2 cut(s)	274, 556
BmuI	ACTGGG	2 cut(s)	274, 556
BoxI	GACNNNNGTC	1 cut(s)	3162
BpiI	GAAGAC	1 cut(s)	575
BplI	GAGNNNNNCTC	2 cut(s)	188, 220
BpmI	CTGGAG	9 cut(s)	227, 367, 1347, 2010, 2115, 2276, 2584, 3143, 3327
Bpu10I	CCTNAGC	1 cut(s)	1671
Bpu14I	TTCGAA	1 cut(s)	1757
BpuEI	CTTGAG	2 cut(s)	2336, 2569
BpuMI	CCSGG	2 cut(s)	1041, 2189
BsaBI	GATNNNNATC	1 cut(s)	1923
BsaHI	GRCGYC	1 cut(s)	613
BsaI	GGTCTC	3 cut(s)	209, 1149, 2007
BsaWI	WCCGGW	1 cut(s)	2020
BsaXI	ACNNNNNCTCC	4 cut(s)	291, 321, 2582, 2612
Bsc4I	CCNNNNNNNGG	1 cut(s)	376
Bse118I	RCCGGY	2 cut(s)	74, 2020
Bse1I	ACTGG	9 cut(s)	244, 280, 350, 551, 736, 1145, 1330, 1684, 3579
Bse3DI	GCAATG	8 cut(s)	338, 1234, 1320, 1539, 1735, 1795, 2794, 3094
Bse8I	GATNNNNATC	1 cut(s)	1923
BseBI	CCWGG	9 cut(s)	35, 371, 873, 1560, 2133, 2294, 2834, 2993, 3149
BseJI	GATNNNNATC	1 cut(s)	1923
BseLI	CCNNNNNNNGG	1 cut(s)	376
BseMI	GCAATG	8 cut(s)	338, 1234, 1320, 1539, 1735, 1795, 2794, 3094
BseMII	CTCAG	6 cut(s)	975, 1160, 1623, 1662, 2760, 3120
BseNI	ACTGG	9 cut(s)	244, 280, 350, 551, 736, 1145, 1330, 1684, 3579
BseRI	GAGGAG	5 cut(s)	41, 134, 315, 627, 1344
BseXI	GCAGC	9 cut(s)	60, 538, 637, 795, 1812, 1991, 2208, 3254, 3607
BseYI	CCCAGC	2 cut(s)	226, 695
BsgI	GTGCAG	2 cut(s)	372, 3684
Bsh1236I	CGCG	2 cut(s)	239, 1501
BshNI	GGYRCC	2 cut(s)	612, 931
BshTI	ACCGGT	1 cut(s)	2020
BsiHKAI	GWGCWC	2 cut(s)	900, 2314
BsiSI	CCGG	5 cut(s)	75, 1041, 1121, 2021, 2188
BslFI	GGGAC	3 cut(s)	1690, 1823, 2204
BslI	CCNNNNNNNGG	1 cut(s)	376
BsmAI	GTCTC	5 cut(s)	209, 1149, 2007, 2133, 2697
BsmFI	GGGAC	3 cut(s)	1690, 1823, 2204
BsmI	GAATGC	5 cut(s)	316, 415, 505, 802, 1765
Bso31I	GGTCTC	3 cut(s)	209, 1149, 2007
Bsp119I	TTCGAA	1 cut(s)	1757
Bsp1286I	GDGCHC	2 cut(s)	900, 2314
Bsp1407I	TGTACA	1 cut(s)	141
BspCNI	CTCAG	6 cut(s)	974, 1159, 1622, 1663, 2759, 3119
BspFNI	CGCG	2 cut(s)	239, 1501
BspHI	TCATGA	1 cut(s)	2281
BspLI	GGNNCC	4 cut(s)	79, 614, 933, 2416
BspMAI	CTGCAG	2 cut(s)	1003, 2122
BspPI	GGATC	4 cut(s)	500, 1031, 1352, 3285
BspQI	GCTCTTC	1 cut(s)	2448
BspT104I	TTCGAA	1 cut(s)	1757
BspT107I	GGYRCC	2 cut(s)	612, 931
BspTI	CTTAAG	1 cut(s)	2513
BspTNI	GGTCTC	3 cut(s)	209, 1149, 2007
BsrBI	CCGCTC	1 cut(s)	1363
BsrDI	GCAATG	8 cut(s)	338, 1234, 1320, 1539, 1735, 1795, 2794, 3094
BsrFI	RCCGGY	2 cut(s)	74, 2020
BsrGI	TGTACA	1 cut(s)	141
BsrI	ACTGG	9 cut(s)	244, 280, 350, 551, 736, 1145, 1330, 1684, 3579
BssAI	RCCGGY	2 cut(s)	74, 2020
BssNAI	GTATAC	1 cut(s)	578
BssNI	GRCGYC	1 cut(s)	613
BssSI	CACGAG	1 cut(s)	1665
BssT1I	CCWWGG	6 cut(s)	790, 1241, 1861, 2225, 3456, 3570
Bst1107I	GTATAC	1 cut(s)	578
Bst2BI	CACGAG	1 cut(s)	1665
Bst2UI	CCWGG	9 cut(s)	35, 371, 873, 1560, 2133, 2294, 2834, 2993, 3149
Bst6I	CTCTTC	3 cut(s)	1185, 1457, 2448
BstACI	GRCGYC	1 cut(s)	613
BstAFI	CTTAAG	1 cut(s)	2513
BstAPI	GCANNNNNTGC	2 cut(s)	752, 2576
BstAUI	TGTACA	1 cut(s)	141
BstBI	TTCGAA	1 cut(s)	1757
BstC8I	GCNNGC	8 cut(s)	676, 958, 1131, 2143, 2592, 2753, 3024, 3217
BstDEI	CTNAG	9 cut(s)	961, 1146, 1550, 1609, 1671, 2614, 2746, 2776, 3106
BstFNI	CGCG	2 cut(s)	239, 1501
BstH2I	RGCGCY	2 cut(s)	616, 3291
BstHHI	GCGC	5 cut(s)	241, 615, 1501, 1539, 3290
BstMAI	GTCTC	5 cut(s)	209, 1149, 2007, 2133, 2697
BstNI	CCWGG	9 cut(s)	35, 371, 873, 1560, 2133, 2294, 2834, 2993, 3149
BstNSI	RCATGY	4 cut(s)	446, 678, 2805, 2949
BstPAI	GACNNNNGTC	1 cut(s)	3162
BstSFI	CTRYAG	4 cut(s)	999, 2118, 3611, 3704
BstUI	CGCG	2 cut(s)	239, 1501
BstV1I	GCAGC	9 cut(s)	60, 538, 637, 795, 1812, 1991, 2208, 3254, 3607
BstV2I	GAAGAC	1 cut(s)	575
BstX2I	RGATCY	3 cut(s)	1036, 3277, 3397
BstXI	CCANNNNNNTGG	2 cut(s)	879, 1724
BstYI	RGATCY	3 cut(s)	1036, 3277, 3397
BstZ17I	GTATAC	1 cut(s)	578
BsuI	GTATCC	1 cut(s)	1258
BtrI	CACGTC	1 cut(s)	139
BtsI	GCAGTG	3 cut(s)	360, 2115, 2572
BtsIMutI	CAGTG	8 cut(s)	251, 343, 360, 544, 729, 1192, 2115, 2572
Cac8I	GCNNGC	8 cut(s)	676, 958, 1131, 2143, 2592, 2753, 3024, 3217
CaiI	CAGNNNCTG	2 cut(s)	2432, 3305
CciI	TCATGA	1 cut(s)	2281
CfoI	GCGC	5 cut(s)	241, 615, 1501, 1539, 3290
Cfr10I	RCCGGY	2 cut(s)	74, 2020
Cfr13I	GGNCC	7 cut(s)	31, 37, 249, 319, 451, 1367, 1556
CseI	GACGC	2 cut(s)	2707, 3277
Csp6I	GTAC	4 cut(s)	142, 689, 1404, 3044
CspAI	ACCGGT	1 cut(s)	2020
CviQI	GTAC	4 cut(s)	142, 689, 1404, 3044
DdeI	CTNAG	9 cut(s)	961, 1146, 1550, 1609, 1671, 2614, 2746, 2776, 3106
DinI	GGCGCC	1 cut(s)	614
DraI	TTTAAA	4 cut(s)	2557, 2736, 3321, 3540
DrdI	GACNNNNNNGTC	1 cut(s)	2021
DseDI	GACNNNNNNGTC	1 cut(s)	2021
EaeI	YGGCCR	1 cut(s)	1464
Eam1104I	CTCTTC	3 cut(s)	1185, 1457, 2448
EarI	CTCTTC	3 cut(s)	1185, 1457, 2448
Eco130I	CCWWGG	6 cut(s)	790, 1241, 1861, 2225, 3456, 3570
Eco147I	AGGCCT	2 cut(s)	1177, 1982
Eco31I	GGTCTC	3 cut(s)	209, 1149, 2007
Eco47I	GGWCC	3 cut(s)	31, 319, 1367
Eco57I	CTGAAG	4 cut(s)	161, 1555, 2601, 3096
EcoRII	CCWGG	9 cut(s)	33, 369, 871, 1558, 2131, 2292, 2832, 2991, 3147
EcoT14I	CCWWGG	6 cut(s)	790, 1241, 1861, 2225, 3456, 3570
EcoT22I	ATGCAT	4 cut(s)	604, 1765, 2174, 2947
EgeI	GGCGCC	1 cut(s)	614
EheI	GGCGCC	1 cut(s)	614
ErhI	CCWWGG	6 cut(s)	790, 1241, 1861, 2225, 3456, 3570
FalI	AAGNNNNNCTT	6 cut(s)	523, 555, 695, 727, 2612, 2644
FaqI	GGGAC	3 cut(s)	1690, 1823, 2204
FauI	CCCGC	3 cut(s)	319, 351, 406
FauNDI	CATATG	1 cut(s)	1582
FbaI	TGATCA	2 cut(s)	1585, 1924
FblI	GTMKAC	1 cut(s)	577
FspBI	CTAG	4 cut(s)	860, 1995, 2093, 2226
GlaI	GCGC	5 cut(s)	240, 614, 1500, 1538, 3289
GsaI	CCCAGC	2 cut(s)	230, 699
GsuI	CTGGAG	9 cut(s)	227, 367, 1347, 2010, 2115, 2276, 2584, 3143, 3327
HaeII	RGCGCY	2 cut(s)	616, 3291
HapII	CCGG	5 cut(s)	75, 1041, 1121, 2021, 2188
HgaI	GACGC	2 cut(s)	2707, 3277
HhaI	GCGC	5 cut(s)	241, 615, 1501, 1539, 3290
Hin1I	GRCGYC	1 cut(s)	613
Hin6I	GCGC	5 cut(s)	239, 613, 1499, 1537, 3288
HinP1I	GCGC	5 cut(s)	239, 613, 1499, 1537, 3288
HincII	GTYRAC	2 cut(s)	951, 1320
HindII	GTYRAC	2 cut(s)	951, 1320
HindIII	AAGCTT	2 cut(s)	173, 2759
HpaI	GTTAAC	1 cut(s)	951
HpaII	CCGG	5 cut(s)	75, 1041, 1121, 2021, 2188
HphI	GGTGA	8 cut(s)	418, 428, 1706, 1928, 2434, 3023, 3136, 3598
Hpy166II	GTNNAC	9 cut(s)	142, 144, 578, 951, 1320, 1820, 1967, 2378, 3144
Hpy8I	GTNNAC	9 cut(s)	142, 144, 578, 951, 1320, 1820, 1967, 2378, 3144
Hpy99I	CGWCG	3 cut(s)	10, 26, 140
HpyCH4IV	ACGT	5 cut(s)	138, 580, 820, 1749, 3559
HpyF3I	CTNAG	9 cut(s)	961, 1146, 1550, 1609, 1671, 2614, 2746, 2776, 3106
HpySE526I	ACGT	5 cut(s)	138, 580, 820, 1749, 3559
Hsp92I	GRCGYC	1 cut(s)	613
HspAI	GCGC	5 cut(s)	239, 613, 1499, 1537, 3288
KasI	GGCGCC	1 cut(s)	612
Ksp22I	TGATCA	2 cut(s)	1585, 1924
KspAI	GTTAAC	1 cut(s)	951
LguI	GCTCTTC	1 cut(s)	2448
LmnI	GCTCC	7 cut(s)	246, 379, 903, 1382, 1991, 2562, 3308
Lsp1109I	GCAGC	9 cut(s)	60, 538, 637, 795, 1812, 1991, 2208, 3254, 3607
MaeI	CTAG	4 cut(s)	860, 1995, 2093, 2226
MaeII	ACGT	5 cut(s)	138, 580, 820, 1749, 3559
MbiI	CCGCTC	1 cut(s)	1363
MfeI	CAATTG	1 cut(s)	1252
MflI	RGATCY	3 cut(s)	1036, 3277, 3397
MhlI	GDGCHC	2 cut(s)	900, 2314
Mly113I	GGCGCC	1 cut(s)	613
MlyI	GAGTC	5 cut(s)	133, 1341, 2245, 3296, 3584
MmeI	TCCRAC	3 cut(s)	2339, 2604, 3061
Mph1103I	ATGCAT	4 cut(s)	604, 1765, 2174, 2947
MslI	CAYNNNNRTG	7 cut(s)	348, 1052, 1416, 1695, 1722, 1940, 3642
MspA1I	CMGCKG	2 cut(s)	956, 2526
MspCI	CTTAAG	1 cut(s)	2513
MspI	CCGG	5 cut(s)	75, 1041, 1121, 2021, 2188
MunI	CAATTG	1 cut(s)	1252
Mva1269I	GAATGC	5 cut(s)	316, 415, 505, 802, 1765
MvaI	CCWGG	9 cut(s)	35, 371, 873, 1560, 2133, 2294, 2834, 2993, 3149
MvnI	CGCG	2 cut(s)	239, 1501
NarI	GGCGCC	1 cut(s)	613
NciI	CCSGG	2 cut(s)	1041, 2189
NdeI	CATATG	1 cut(s)	1582
NlaIV	GGNNCC	4 cut(s)	79, 614, 933, 2416
NmuCI	GTSAC	8 cut(s)	904, 1205, 1333, 2422, 2489, 2719, 3011, 3586
NsiI	ATGCAT	4 cut(s)	604, 1765, 2174, 2947
NspI	RCATGY	4 cut(s)	446, 678, 2805, 2949
NspV	TTCGAA	1 cut(s)	1757
OliI	CACNNNNGTG	1 cut(s)	348
PaeI	GCATGC	1 cut(s)	678
PagI	TCATGA	1 cut(s)	2281
PceI	AGGCCT	2 cut(s)	1177, 1982
PciI	ACATGT	1 cut(s)	442
PciSI	GCTCTTC	1 cut(s)	2448
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 1464
PctI	GAATGC	5 cut(s)	316, 415, 505, 802, 1765
PfoI	TCCNGGA	1 cut(s)	2131
PinAI	ACCGGT	1 cut(s)	2020
PleI	GAGTC	5 cut(s)	132, 1340, 2244, 3296, 3583
PluTI	GGCGCC	1 cut(s)	616
PpsI	GAGTC	5 cut(s)	132, 1340, 2244, 3296, 3583
PscI	ACATGT	1 cut(s)	442
PshAI	GACNNNNGTC	1 cut(s)	3162
PsiI	TTATAA	2 cut(s)	558, 2481
Psp1406I	AACGTT	1 cut(s)	820
Psp6I	CCWGG	9 cut(s)	33, 369, 871, 1558, 2131, 2292, 2832, 2991, 3147
PspFI	CCCAGC	2 cut(s)	226, 695
PspGI	CCWGG	9 cut(s)	33, 369, 871, 1558, 2131, 2292, 2832, 2991, 3147
PspN4I	GGNNCC	4 cut(s)	79, 614, 933, 2416
PspPI	GGNCC	7 cut(s)	31, 37, 249, 319, 451, 1367, 1556
PstI	CTGCAG	2 cut(s)	1003, 2122
PstNI	CAGNNNCTG	2 cut(s)	2432, 3305
PsuI	RGATCY	3 cut(s)	1036, 3277, 3397
PvuII	CAGCTG	1 cut(s)	956
RsaI	GTAC	4 cut(s)	143, 690, 1405, 3045
RsaNI	GTAC	4 cut(s)	142, 689, 1404, 3044
RseI	CAYNNNNRTG	7 cut(s)	348, 1052, 1416, 1695, 1722, 1940, 3642
SapI	GCTCTTC	1 cut(s)	2448
Sau96I	GGNCC	7 cut(s)	31, 37, 249, 319, 451, 1367, 1556
SchI	GAGTC	5 cut(s)	133, 1341, 2245, 3296, 3584
SduI	GDGCHC	2 cut(s)	900, 2314
SfcI	CTRYAG	4 cut(s)	999, 2118, 3611, 3704
SfoI	GGCGCC	1 cut(s)	614
SfuI	TTCGAA	1 cut(s)	1757
SinI	GGWCC	3 cut(s)	31, 319, 1367
SmiMI	CAYNNNNRTG	7 cut(s)	348, 1052, 1416, 1695, 1722, 1940, 3642
SmlI	CTYRAG	3 cut(s)	2315, 2513, 2548
SmoI	CTYRAG	3 cut(s)	2315, 2513, 2548
SphI	GCATGC	1 cut(s)	678
SseBI	AGGCCT	2 cut(s)	1177, 1982
SspDI	GGCGCC	1 cut(s)	612
SspI	AATATT	1 cut(s)	1507
SspMI	CTAG	4 cut(s)	860, 1995, 2093, 2226
StuI	AGGCCT	2 cut(s)	1177, 1982
StyI	CCWWGG	6 cut(s)	790, 1241, 1861, 2225, 3456, 3570
TaiI	ACGT	5 cut(s)	141, 583, 823, 1752, 3562
TaqI	TCGA	6 cut(s)	15, 24, 802, 1199, 1757, 2459
TaqII	GACCGA	2 cut(s)	233, 1355
TatI	WGTACW	2 cut(s)	141, 3043
TauI	GCSGC	7 cut(s)	68, 152, 155, 254, 275, 278, 452
TscAI	CASTG	8 cut(s)	251, 350, 360, 551, 736, 1199, 2122, 2572
TseFI	GTSAC	8 cut(s)	904, 1205, 1333, 2422, 2489, 2719, 3011, 3586
TseI	GCWGC	9 cut(s)	48, 551, 650, 808, 1800, 2004, 2221, 3242, 3620
Tsp45I	GTSAC	8 cut(s)	904, 1205, 1333, 2422, 2489, 2719, 3011, 3586
TspGWI	ACGGA	2 cut(s)	70, 1882
TspRI	CASTG	8 cut(s)	251, 350, 360, 551, 736, 1199, 2122, 2572
Vha464I	CTTAAG	1 cut(s)	2513
VpaK11BI	GGWCC	3 cut(s)	31, 319, 1367
XapI	RAATTY	8 cut(s)	1347, 1753, 2536, 2974, 3055, 3421, 3505, 3653
XceI	RCATGY	4 cut(s)	446, 678, 2805, 2949
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1811
XmaJI	CCTAGG	1 cut(s)	2225
XmiI	GTMKAC	1 cut(s)	577
XspI	CTAG	4 cut(s)	860, 1995, 2093, 2226
Zsp2I	ATGCAT	4 cut(s)	604, 1765, 2174, 2947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.