Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Reverse (-)

1360952 .. 1365956

Gene Structure 5005 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1018500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGTCGTCGGAGATCGAGGTCGTCGAGGATGAGGTCCAAACCGGTGATCCTGGATCCGTCTCTGCCAATTCAACGAACAACAATGGCGACGCTAATCGGATTGGGGAAGAGAGTCTGCGAAACGATGTGTATACGGCGGCTGCGTATGGGGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGTTGCTCTGTTTCCGAGACCGATGGTCTCGGCTACTATGCCCTCCAGTGGGCCGCTCTCAACAACCGCACTGCCGCCGCCCAGTATATTATTGAGCATGGAGGAGATGTGAATGCCACAGATCATACGGGGCAAACAGCATTACACTGGAGTGCGGTGCGTGGTGCAATCCAGGTTGCGGAGCTATTACTCCAAGAGGGTGCTCGGGTGAATGCTGTCGATATGTATGGCTATCAGACTACACATGTTGCGGCTCAATATGGTCAGACAGCTTTCCTGTACCATATTGTTTCAAAATGGAATGCTGACCCTGATGTGCCTGATAATGACGGGAGAAGCCCTTTACACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTATTTCTGGATGCATATAAAGGACGCCAAGATAAGGAGGGTTGCACTCCTTTGCATTGGGCTGCTATCAGGGGTAACTTGGAGGCATGTACAGTGTTGGTACAGGCTGGAAAGAAGGACGACTTAATGGTTACAGACAACACTGGCCTTACACCAGCACAACTTGCCGCTGATAAGAATCACAGACAAGTTGCCTTTTTCCTTGGGAATGCTAGAAGACTGCTTGACAAACGCTGTGATGGGAACAGTCGTCTTGGAAGAATTTCAAAACTAGGACTTGCGCCACTACTTTGGTGTATAATCTTTGTGCTCCTGGTGACCTATACTCATTCTGTCATTATGGCACCAAGTCTGCCAAAATTAACAGCTGGATCAGTTTTTGTTGCATGGCTAGGAGTTTTCCTAGCAACATCCGGGCTTGTTATGTTCTATAGATGCAGCAGCAAGGATCCAGGTTACATCCGAATGAATGTCCATGATTCACAAAATATGAAAGATGATGAGCCTCTTTTGAAGATTGAGATAAATAATCCTGCCTTGCTAGCTGGTAATTGGTCTCAGCTCTGTGCAACGTGCAAGATTGTCAGGCCTCTTCGTGCAAAGCACTGTCCAACTTGTGATCGTTGTGTTGAACAATTTGACCACCATTGCCCCTGGGTATCCAATTGCATTGGCAAGAAAAACAAGTGGGATTTCTTTGCTTTCCTTGTTCTGGAAGTTTTGGCGATGGTGTTTACTGGAGGGGTTACTCTTATGAGAGTTTCGAGCGATCCATTGTGTCCATCTTCTTTTGGAGCATGGATCAATTTTGTTGCTACTAGACATGTGGGTGCTCTTTCATTTTTTATAATGGATTTCTTCCTCTTCTTTGGCGTGGTGGTTTTGACCGTTGTCCAAGCTTCTCAGATATCACGGAACATTACAACTAACGAAATGGCAAACATGATGCGCTATAACTACCTTCGAGGCCCAGGTGGTAGGTTCAGAAATCCATTCGATCATGGGATAAGGAAGAATTGTTCGGATTTCTTGATCAAGGGTTACAACGAAGATGTGGAACACATTGAAGAACCGGCTCATGATGAGGAGGGAATTGGGATGAGGGATATAAATTCTTCAAACTTGCAGAAAGGGGACGCCTATTCTCACCGCACAAACGGCAACAGTCACGCTGCAATCGATGTAAATTCTAATTCAACACCACATCACGGTCACGTTCATTCTGCCCATTGCAGCCATAGTAACAACCACGGGAAATCGAAAACTGAAAACATTCCGGTAGGATTGGGTCTTGGCCTGGGACGGAACAGTGCCCGGTCTGTTGTAGCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

637

Amino Acids

69.98

Weight (kDa)

6.35

Isoelectric Point (pI)

30.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	45 - 136	8.1e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	79 - 126	3.6e-07	Ankyrin repeats (many copies)
Ank	PF00023	106 - 136	7.2e-06	Ankyrin repeat
Ank_2	PF12796	144 - 230	3.2e-16	Ankyrin repeats (3 copies)
DHHC	PF01529	379 - 506	8.9e-31	DHHC palmitoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	546, 1424
AccB1I	GGYRCC	1 cut(s)	919
AccBSI	CCGCTC	1 cut(s)	242
AccI	GTMKAC	2 cut(s)	131, 565
AclWI	GGATC	8 cut(s)	41, 48, 61, 955, 1019, 1032, 1340, 1385
AcsI	RAATTY	3 cut(s)	837, 1687, 1762
AcuI	CTGAAG	1 cut(s)	149
AcyI	GRCGYC	2 cut(s)	601, 1713
AfaI	GTAC	3 cut(s)	467, 667, 678
AfiI	CCNNNNNNNGG	6 cut(s)	234, 235, 364, 610, 1288, 1784
AflIII	ACRYGT	2 cut(s)	430, 1399
AgeI	ACCGGT	1 cut(s)	41
AgsI	TTSAA	8 cut(s)	72, 480, 843, 1090, 1208, 1643, 1695, 1773
AhdI	GACNNNNNGTC	1 cut(s)	210
AjnI	CCWGG	7 cut(s)	49, 357, 888, 1027, 1229, 1546, 1872
AleI	CACNNNNGTG	1 cut(s)	336
AloI	GAACNNNNNNTCC	2 cut(s)	1579, 1611
AluBI	AGCT	8 cut(s)	163, 370, 458, 944, 1121, 1138, 1475, 1904
AluI	AGCT	8 cut(s)	163, 370, 458, 944, 1121, 1138, 1475, 1904
Alw21I	GWGCWC	3 cut(s)	391, 888, 1411
Alw26I	GTCTC	4 cut(s)	64, 197, 218, 1137
AlwI	GGATC	8 cut(s)	41, 48, 61, 955, 1019, 1032, 1340, 1385
Ama87I	CYCGRG	1 cut(s)	390
AoxI	GGCC	5 cut(s)	237, 721, 1163, 1543, 1870
ApeKI	GCWGC	7 cut(s)	140, 539, 638, 1014, 1017, 1748, 1809
ApoI	RAATTY	3 cut(s)	837, 1687, 1762
AsiGI	ACCGGT	1 cut(s)	41
Asp700I	GAANNNNTTC	2 cut(s)	838, 1848
AspLEI	GCGC	2 cut(s)	859, 1527
AspS9I	GGNCC	3 cut(s)	34, 237, 1544
AsuC2I	CCSGG	2 cut(s)	991, 1891
AsuHPI	GGTGA	4 cut(s)	56, 406, 904, 1715
AsuNHI	GCTAGC	1 cut(s)	1117
AvaI	CYCGRG	1 cut(s)	390
AvaII	GGWCC	1 cut(s)	34
BaeGI	GKGCMC	1 cut(s)	1891
BamHI	GGATCC	2 cut(s)	53, 1024
BanI	GGYRCC	1 cut(s)	919
BbsI	GAAGAC	2 cut(s)	563, 799
Bbv12I	GWGCWC	3 cut(s)	391, 888, 1411
BbvI	GCAGC	7 cut(s)	127, 526, 625, 1026, 1029, 1735, 1821
BccI	CCATC	4 cut(s)	203, 809, 1297, 1366
BceAI	ACGGC	2 cut(s)	150, 1750
BciT130I	CCWGG	7 cut(s)	51, 359, 890, 1029, 1231, 1548, 1874
BciVI	GTATCC	1 cut(s)	1246
BclI	TGATCA	1 cut(s)	1608
BcnI	CCSGG	2 cut(s)	991, 1891
BcoDI	GTCTC	4 cut(s)	64, 197, 218, 1137
BfaI	CTAG	6 cut(s)	789, 848, 968, 980, 1118, 1395
BfmI	CTRYAG	1 cut(s)	1006
BfuI	GTATCC	1 cut(s)	1246
Bme1390I	CCNGG	9 cut(s)	51, 359, 890, 991, 1029, 1231, 1548, 1874, 1891
Bme18I	GGWCC	1 cut(s)	34
BmeRI	GACNNNNNGTC	1 cut(s)	210
BmeT110I	CYCGRG	1 cut(s)	390
BmgT120I	GGNCC	3 cut(s)	34, 237, 1544
BmiI	GGNNCC	3 cut(s)	55, 921, 1026
BmrFI	CCNGG	9 cut(s)	51, 359, 890, 991, 1029, 1231, 1548, 1874, 1891
BmrI	ACTGGG	2 cut(s)	262, 544
BmsI	GCATC	3 cut(s)	577, 1001, 1512
BmtI	GCTAGC	1 cut(s)	1121
BmuI	ACTGGG	2 cut(s)	262, 544
BoxI	GACNNNNGTC	1 cut(s)	1466
BpiI	GAAGAC	2 cut(s)	563, 799
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	3 cut(s)	215, 355, 1335
BpuMI	CCSGG	2 cut(s)	991, 1891
Bsa29I	ATCGAT	1 cut(s)	1755
BsaBI	GATNNNNATC	1 cut(s)	753
BsaHI	GRCGYC	2 cut(s)	601, 1713
BsaI	GGTCTC	3 cut(s)	197, 218, 1137
BsaJI	CCNNGG	6 cut(s)	778, 1229, 1230, 1546, 1825, 1873
BsaWI	WCCGGW	2 cut(s)	41, 1852
BsaXI	ACNNNNNCTCC	2 cut(s)	279, 309
Bsc4I	CCNNNNNNNGG	6 cut(s)	234, 235, 364, 610, 1288, 1784
Bse118I	RCCGGY	2 cut(s)	41, 1648
Bse1I	ACTGG	6 cut(s)	232, 268, 338, 539, 724, 1318
Bse3DI	GCAATG	2 cut(s)	1222, 1804
Bse8I	GATNNNNATC	1 cut(s)	753
BseBI	CCWGG	7 cut(s)	51, 359, 890, 1029, 1231, 1548, 1874
BseCI	ATCGAT	1 cut(s)	1755
BseDI	CCNNGG	6 cut(s)	778, 1229, 1230, 1546, 1825, 1873
BseGI	GGATG	5 cut(s)	34, 592, 986, 1035, 1680
BseJI	GATNNNNATC	1 cut(s)	753
BseLI	CCNNNNNNNGG	6 cut(s)	234, 235, 364, 610, 1288, 1784
BseMI	GCAATG	2 cut(s)	1222, 1804
BseMII	CTCAG	2 cut(s)	1148, 1493
BseNI	ACTGG	6 cut(s)	232, 268, 338, 539, 724, 1318
BseRI	GAGGAG	2 cut(s)	303, 1676
BseSI	GKGCMC	1 cut(s)	1891
BseXI	GCAGC	7 cut(s)	127, 526, 625, 1026, 1029, 1735, 1821
BshFI	GGCC	5 cut(s)	239, 723, 1165, 1545, 1872
BshNI	GGYRCC	1 cut(s)	919
BshTI	ACCGGT	1 cut(s)	41
BshVI	ATCGAT	1 cut(s)	1755
BsiHKAI	GWGCWC	3 cut(s)	391, 888, 1411
BsiHKCI	CYCGRG	1 cut(s)	390
BsiSI	CCGG	5 cut(s)	42, 990, 1649, 1853, 1891
BslFI	GGGAC	2 cut(s)	1724, 1890
BslI	CCNNNNNNNGG	6 cut(s)	234, 235, 364, 610, 1288, 1784
BsmAI	GTCTC	4 cut(s)	64, 197, 218, 1137
BsmBI	CGTCTC	1 cut(s)	64
BsmFI	GGGAC	2 cut(s)	1724, 1890
BsmI	GAATGC	4 cut(s)	304, 403, 493, 790
BsnI	GGCC	5 cut(s)	239, 723, 1165, 1545, 1872
Bso31I	GGTCTC	3 cut(s)	197, 218, 1137
BsoBI	CYCGRG	1 cut(s)	390
Bsp1286I	GDGCHC	4 cut(s)	391, 888, 1411, 1891
Bsp1407I	TGTACA	1 cut(s)	665
BspANI	GGCC	5 cut(s)	239, 723, 1165, 1545, 1872
BspCNI	CTCAG	2 cut(s)	1147, 1492
BspDI	ATCGAT	1 cut(s)	1755
BspHI	TCATGA	2 cut(s)	1654, 1907
BspLI	GGNNCC	3 cut(s)	55, 921, 1026
BspOI	GCTAGC	1 cut(s)	1121
BspPI	GGATC	8 cut(s)	41, 48, 61, 955, 1019, 1032, 1340, 1385
BspT107I	GGYRCC	1 cut(s)	919
BspTNI	GGTCTC	3 cut(s)	197, 218, 1137
BsrBI	CCGCTC	1 cut(s)	242
BsrDI	GCAATG	2 cut(s)	1222, 1804
BsrFI	RCCGGY	2 cut(s)	41, 1648
BsrGI	TGTACA	1 cut(s)	665
BsrI	ACTGG	6 cut(s)	232, 268, 338, 539, 724, 1318
BssAI	RCCGGY	2 cut(s)	41, 1648
BssECI	CCNNGG	6 cut(s)	778, 1229, 1230, 1546, 1825, 1873
BssNAI	GTATAC	2 cut(s)	132, 566
BssNI	GRCGYC	2 cut(s)	601, 1713
BssT1I	CCWWGG	1 cut(s)	778
Bst1107I	GTATAC	2 cut(s)	132, 566
Bst2UI	CCWGG	7 cut(s)	51, 359, 890, 1029, 1231, 1548, 1874
Bst4CI	ACNGT	7 cut(s)	670, 824, 1184, 1465, 1742, 1787, 1886
Bst6I	CTCTTC	3 cut(s)	102, 1173, 1445
BstACI	GRCGYC	2 cut(s)	601, 1713
BstAPI	GCANNNNNTGC	1 cut(s)	740
BstAUI	TGTACA	1 cut(s)	665
BstC8I	GCNNGC	1 cut(s)	1119
BstDEI	CTNAG	2 cut(s)	1134, 1479
BstDSI	CCRYGG	1 cut(s)	1825
BstEII	GGTNACC	1 cut(s)	892
BstF5I	GGATG	5 cut(s)	34, 592, 986, 1035, 1680
BstHHI	GCGC	2 cut(s)	859, 1527
BstMAI	GTCTC	4 cut(s)	64, 197, 218, 1137
BstMWI	GCNNNNNNNGC	3 cut(s)	740, 1118, 1734
BstNI	CCWGG	7 cut(s)	51, 359, 890, 1029, 1231, 1548, 1874
BstNSI	RCATGY	3 cut(s)	434, 666, 1403
BstPAI	GACNNNNGTC	1 cut(s)	1466
BstPI	GGTNACC	1 cut(s)	892
BstSCI	CCNGG	9 cut(s)	49, 357, 888, 989, 1027, 1229, 1546, 1872, 1889
BstSFI	CTRYAG	1 cut(s)	1006
BstSLI	GKGCMC	1 cut(s)	1891
BstV1I	GCAGC	7 cut(s)	127, 526, 625, 1026, 1029, 1735, 1821
BstV2I	GAAGAC	2 cut(s)	563, 799
BstX2I	RGATCY	2 cut(s)	53, 1024
BstXI	CCANNNNNNTGG	1 cut(s)	867
BstYI	RGATCY	2 cut(s)	53, 1024
BstZ17I	GTATAC	2 cut(s)	132, 566
Bsu15I	ATCGAT	1 cut(s)	1755
BsuI	GTATCC	1 cut(s)	1246
BsuRI	GGCC	5 cut(s)	239, 723, 1165, 1545, 1872
BsuTUI	ATCGAT	1 cut(s)	1755
BtgI	CCRYGG	1 cut(s)	1825
BtgZI	GCGATG	1 cut(s)	1316
BtsCI	GGATG	5 cut(s)	34, 592, 986, 1035, 1680
BtsI	GCAGTG	1 cut(s)	255
BtsIMutI	CAGTG	8 cut(s)	239, 255, 331, 532, 675, 717, 1180, 1891
Cac8I	GCNNGC	1 cut(s)	1119
CciI	TCATGA	2 cut(s)	1654, 1907
CfoI	GCGC	2 cut(s)	859, 1527
Cfr10I	RCCGGY	2 cut(s)	41, 1648
Cfr13I	GGNCC	3 cut(s)	34, 237, 1544
ClaI	ATCGAT	1 cut(s)	1755
CseI	GACGC	3 cut(s)	98, 609, 1721
Csp6I	GTAC	3 cut(s)	466, 666, 677
CspAI	ACCGGT	1 cut(s)	41
CviQI	GTAC	3 cut(s)	466, 666, 677
DdeI	CTNAG	2 cut(s)	1134, 1479
DriI	GACNNNNNGTC	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	102, 1173, 1445
Eam1105I	GACNNNNNGTC	1 cut(s)	210
EarI	CTCTTC	3 cut(s)	102, 1173, 1445
Eco130I	CCWWGG	1 cut(s)	778
Eco147I	AGGCCT	1 cut(s)	1165
Eco31I	GGTCTC	3 cut(s)	197, 218, 1137
Eco32I	GATATC	1 cut(s)	1485
Eco47I	GGWCC	1 cut(s)	34
Eco57I	CTGAAG	1 cut(s)	149
Eco88I	CYCGRG	1 cut(s)	390
Eco91I	GGTNACC	1 cut(s)	892
EcoO65I	GGTNACC	1 cut(s)	892
EcoRII	CCWGG	7 cut(s)	49, 357, 888, 1027, 1229, 1546, 1872
EcoRV	GATATC	1 cut(s)	1485
EcoT14I	CCWWGG	1 cut(s)	778
EcoT22I	ATGCAT	1 cut(s)	592
ErhI	CCWWGG	1 cut(s)	778
Esp3I	CGTCTC	1 cut(s)	64
FalI	AAGNNNNNCTT	2 cut(s)	683, 715
FaqI	GGGAC	2 cut(s)	1724, 1890
FbaI	TGATCA	1 cut(s)	1608
FblI	GTMKAC	2 cut(s)	131, 565
FokI	GGATG	5 cut(s)	41, 599, 973, 1022, 1687
FspBI	CTAG	6 cut(s)	789, 848, 968, 980, 1118, 1395
GlaI	GCGC	2 cut(s)	858, 1526
GsuI	CTGGAG	3 cut(s)	215, 355, 1335
HaeIII	GGCC	5 cut(s)	239, 723, 1165, 1545, 1872
HapII	CCGG	5 cut(s)	42, 990, 1649, 1853, 1891
HgaI	GACGC	3 cut(s)	98, 609, 1721
HhaI	GCGC	2 cut(s)	859, 1527
Hin1I	GRCGYC	2 cut(s)	601, 1713
Hin6I	GCGC	2 cut(s)	857, 1525
HinP1I	GCGC	2 cut(s)	857, 1525
HindIII	AAGCTT	2 cut(s)	161, 1473
HinfI	GANTC	3 cut(s)	112, 754, 1055
HpaII	CCGG	5 cut(s)	42, 990, 1649, 1853, 1891
HphI	GGTGA	4 cut(s)	56, 406, 904, 1715
Hpy166II	GTNNAC	3 cut(s)	132, 566, 1311
Hpy188III	TCNNGA	5 cut(s)	584, 1289, 1606, 1655, 1908
Hpy8I	GTNNAC	3 cut(s)	132, 566, 1311
Hpy99I	CGWCG	3 cut(s)	10, 26, 92
HpyAV	CCTTC	2 cut(s)	685, 1547
HpyCH4III	ACNGT	7 cut(s)	670, 824, 1184, 1465, 1742, 1787, 1886
HpyCH4IV	ACGT	3 cut(s)	568, 1148, 1791
HpyF10VI	GCNNNNNNNGC	3 cut(s)	740, 1118, 1734
HpyF3I	CTNAG	2 cut(s)	1134, 1479
HpySE526I	ACGT	3 cut(s)	568, 1148, 1791
Hsp92I	GRCGYC	2 cut(s)	601, 1713
HspAI	GCGC	2 cut(s)	857, 1525
Ksp22I	TGATCA	1 cut(s)	1608
LmnI	GCTCC	3 cut(s)	367, 891, 1370
Lsp1109I	GCAGC	7 cut(s)	127, 526, 625, 1026, 1029, 1735, 1821
LweI	GCATC	3 cut(s)	577, 1001, 1512
MaeI	CTAG	6 cut(s)	789, 848, 968, 980, 1118, 1395
MaeII	ACGT	3 cut(s)	568, 1148, 1791
MaeIII	GTNAC	9 cut(s)	650, 706, 892, 1031, 1321, 1616, 1742, 1787, 1817
MbiI	CCGCTC	1 cut(s)	242
MfeI	CAATTG	1 cut(s)	1240
MflI	RGATCY	2 cut(s)	53, 1024
MhlI	GDGCHC	4 cut(s)	391, 888, 1411, 1891
MlyI	GAGTC	1 cut(s)	121
MmeI	TCCRAC	1 cut(s)	1211
Mph1103I	ATGCAT	1 cut(s)	592
MroXI	GAANNNNTTC	2 cut(s)	838, 1848
MseI	TTAA	2 cut(s)	701, 938
MslI	CAYNNNNRTG	3 cut(s)	336, 1040, 1404
MspA1I	CMGCKG	2 cut(s)	746, 944
MspI	CCGG	5 cut(s)	42, 990, 1649, 1853, 1891
MspR9I	CCNGG	9 cut(s)	51, 359, 890, 991, 1029, 1231, 1548, 1874, 1891
MunI	CAATTG	1 cut(s)	1240
Mva1269I	GAATGC	4 cut(s)	304, 403, 493, 790
MvaI	CCWGG	7 cut(s)	51, 359, 890, 1029, 1231, 1548, 1874
MwoI	GCNNNNNNNGC	3 cut(s)	740, 1118, 1734
NciI	CCSGG	2 cut(s)	991, 1891
NheI	GCTAGC	1 cut(s)	1117
NlaIV	GGNNCC	3 cut(s)	55, 921, 1026
NmeAIII	GCCGAG	1 cut(s)	195
NmuCI	GTSAC	3 cut(s)	892, 1742, 1787
NsiI	ATGCAT	1 cut(s)	592
NspI	RCATGY	3 cut(s)	434, 666, 1403
OliI	CACNNNNGTG	1 cut(s)	336
PagI	TCATGA	2 cut(s)	1654, 1907
PasI	CCCWGGG	1 cut(s)	1230
PceI	AGGCCT	1 cut(s)	1165
PciI	ACATGT	2 cut(s)	430, 1399
PcsI	WCGNNNNNNNCGW	3 cut(s)	21, 140, 1752
PctI	GAATGC	4 cut(s)	304, 403, 493, 790
PdmI	GAANNNNTTC	2 cut(s)	838, 1848
PfeI	GAWTC	2 cut(s)	754, 1055
PfoI	TCCNGGA	1 cut(s)	49
PinAI	ACCGGT	1 cut(s)	41
PleI	GAGTC	1 cut(s)	120
PpsI	GAGTC	1 cut(s)	120
PscI	ACATGT	2 cut(s)	430, 1399
PshAI	GACNNNNGTC	1 cut(s)	1466
PsiI	TTATAA	2 cut(s)	546, 1424
Psp6I	CCWGG	7 cut(s)	49, 357, 888, 1027, 1229, 1546, 1872
PspEI	GGTNACC	1 cut(s)	892
PspGI	CCWGG	7 cut(s)	49, 357, 888, 1027, 1229, 1546, 1872
PspN4I	GGNNCC	3 cut(s)	55, 921, 1026
PspPI	GGNCC	3 cut(s)	34, 237, 1544
PsuI	RGATCY	2 cut(s)	53, 1024
PvuII	CAGCTG	1 cut(s)	944
RsaI	GTAC	3 cut(s)	467, 667, 678
RsaNI	GTAC	3 cut(s)	466, 666, 677
RseI	CAYNNNNRTG	3 cut(s)	336, 1040, 1404
SaqAI	TTAA	2 cut(s)	701, 938
Sau96I	GGNCC	3 cut(s)	34, 237, 1544
SchI	GAGTC	1 cut(s)	121
ScrFI	CCNGG	9 cut(s)	51, 359, 890, 991, 1029, 1231, 1548, 1874, 1891
SduI	GDGCHC	4 cut(s)	391, 888, 1411, 1891
SfaNI	GCATC	3 cut(s)	577, 1001, 1512
SfcI	CTRYAG	1 cut(s)	1006
SinI	GGWCC	1 cut(s)	34
SmiMI	CAYNNNNRTG	3 cut(s)	336, 1040, 1404
SseBI	AGGCCT	1 cut(s)	1165
SspMI	CTAG	6 cut(s)	789, 848, 968, 980, 1118, 1395
StuI	AGGCCT	1 cut(s)	1165
StyD4I	CCNGG	9 cut(s)	49, 357, 888, 989, 1027, 1229, 1546, 1872, 1889
StyI	CCWWGG	1 cut(s)	778
TaaI	ACNGT	7 cut(s)	670, 824, 1184, 1465, 1742, 1787, 1886
TaiI	ACGT	3 cut(s)	571, 1151, 1794
TaqI	TCGA	8 cut(s)	15, 24, 405, 1340, 1540, 1572, 1755, 1835
TaqII	GACCGA	1 cut(s)	221
TatI	WGTACW	1 cut(s)	665
TauI	GCSGC	6 cut(s)	140, 242, 263, 266, 440, 746
TfiI	GAWTC	2 cut(s)	754, 1055
Tru1I	TTAA	2 cut(s)	701, 938
Tru9I	TTAA	2 cut(s)	701, 938
TscAI	CASTG	8 cut(s)	239, 262, 338, 539, 675, 724, 1187, 1891
TseFI	GTSAC	3 cut(s)	892, 1742, 1787
TseI	GCWGC	7 cut(s)	140, 539, 638, 1014, 1017, 1748, 1809
Tsp45I	GTSAC	3 cut(s)	892, 1742, 1787
TspDTI	ATGAA	5 cut(s)	1058, 1082, 1404, 1784, 1896
TspGWI	ACGGA	3 cut(s)	46, 1504, 1894
TspRI	CASTG	8 cut(s)	239, 262, 338, 539, 675, 724, 1187, 1891
VpaK11BI	GGWCC	1 cut(s)	34
XapI	RAATTY	3 cut(s)	837, 1687, 1762
XceI	RCATGY	3 cut(s)	434, 666, 1403
XmiI	GTMKAC	2 cut(s)	131, 565
XmnI	GAANNNNTTC	2 cut(s)	838, 1848
XspI	CTAG	6 cut(s)	789, 848, 968, 980, 1118, 1395
Zsp2I	ATGCAT	1 cut(s)	592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.