Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Reverse (-)

1068014 .. 1073525

Gene Structure 5512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g02030.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGTCGTCGGAGATCGAGGTCGTCGAGGAGGAGACCCGCCCCGATCACGAATCGTCGGCGGCGGCCGCCTCGGGAAATTCAGGCGGAGAAGCGAATGGGGTGATTGGGGAGGAGAGTCTCCGGAACGACGTGTACACGGCGACGGCGTACGGCGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGTTGCTCGGTTTCCGAGACCGATGGGCTTGGCTACTACGCGCTCCAGTGGGCCGCGCTTAACAACCGAACTGCCGCCGCTCAGTATATAATAGAGCACGGAGGAGATGTGAATGCGAAGGATCATGCGGGGCAGACGGCATTGCACTGGAGTGCTGTGCGGGGTGCTATCCAGGTTGCGGAGCTCTTACTCCAAGAGGGTGCTCGGGTGAATGCCGAGGATATGTATGGCTATCAGACTACACATGTGGCAGCCCAATACGGTCAGACTGCTTTCCTATATCATATTGTTTCGAAATGGAATGCTGACCCTGATGTTCCTGATAATGATGGAAGAAGCCCTTTGCACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTGTTTCTGGATGCATATAGAGGGCGCCAAGATAAAGAGGGTTGCACTCCTCTACATTGGGCTGCGATCAGGGGTAACTTGGAGGCATGCACGGTGTTGGTACAAGCTGGGAAAAAGGAGGACTTGATGGTTACTGATAATACTGGCCTTACACCAGCCCAACTTGCTTCTGATAAAAACCACAGACAAGTTGCTTTTTTCCTTGGGAATGCTCGAAGGTTGCTTGACAAACGTTGTGATGGGAATAGTCGTCTAGGTCAACTAACAAAGCTAGGACTTGCACCAGCACTTTGGTGTGTAATCTTTGTGCTCCTTGTGACCTATAGTCATTCTGTTATCATGGCTCCAAATCTGCCAAAGTTAACAGCCGGCTCAGTTTTTATTGCATGGCTTGGAGTTTTCCTAGCAACTGCAGGGCTGGTTATGTTATATCGGTGTAGCAGTAAAGATCCCGGTTACATCCGAATGAATATCCATGATTCACAAAATATGAAAGATGATGAACCTCTTTTGAAGATTGAGATAAATAATCCGGCCTTGCTAGCTGGTAACTGGTCTCAGCTCTGTGCAACTTGCAAGATTGTCAGGCCTCTTCGTGCAAAGCATTGTTCTACTTGTGACCGTTGTGTTGAACAATTTGACCACCATTGCCCTTGGGTATCCAATTGCATTGGCAAGAAAAACAAATTGGATTTCTTTATATTCCTTGTTCTTGAAGTTTTGGCGATGTTGACTACTGGAGGAGTCACTGTTACGAGAATTTGGAGTGATCCACTCAGTCCATCTTCATTTGGAGCATGGCTTAATCATGTTGGTACAAATCATTTTGGTGCTATATCGTTTTTGATTGTTGATTTCTTCCTCTTCTTTGGTGTGGCTGTTCTGACTGTTGTACAAGCTTCTCAAATTGCGCGAAATATTACAACTAATGAAATGGCAAATGCAATGCGCTATAACTACCTTAGAGGGCCAGGGGGTCACTTCAGAAACCCATATGATCATGGGACCAAGAAGAACTGCTCAGATTTCTTGATCAAGGGCTACAATGAAGATGTGGAATTGATTGAAGAATCAGCTCGTGATGAGGGGACTGGAATGAGGCACATGACTAATTCAGCCTTGCAAAATGGCGATTCCCATTCTCACCACACAAATGGCAATGGCCATGTTGCAATCAACGTAAATTCGAATGCATCTGCACATCAAGGTCATGTTCATTCTGCCCATTGCAGCCATGGCAACCATGGTAAACCAAAAACTGATAATGTACCCTTAGGCTTGGGTCTTGGCCTTGGACGGAACGCTTCCCGACCTGCTGGGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

635

Amino Acids

69.21

Weight (kDa)

6.29

Isoelectric Point (pI)

29.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	47 - 137	5.1e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	80 - 127	6.9e-07	Ankyrin repeats (many copies)
Ank_2	PF12796	145 - 231	3.4e-16	Ankyrin repeats (3 copies)
Ank_2	PF12796	201 - 261	4.1e-08	Ankyrin repeats (3 copies)
DHHC	PF01529	380 - 507	1.8e-32	DHHC palmitoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	549
Acc36I	ACCTGC	1 cut(s)	1900
AccB1I	GGYRCC	1 cut(s)	603
AccBSI	CCGCTC	1 cut(s)	269
AccI	GTMKAC	1 cut(s)	568
AccII	CGCG	3 cut(s)	230, 245, 1492
AccIII	TCCGGA	1 cut(s)	120
AclI	AACGTT	1 cut(s)	811
AclWI	GGATC	3 cut(s)	318, 1022, 1343
AcoI	YGGCCR	2 cut(s)	63, 1741
AcsI	RAATTY	3 cut(s)	76, 1338, 1762
AcuI	CTGAAG	2 cut(s)	152, 1546
AcyI	GRCGYC	1 cut(s)	604
AfaI	GTAC	6 cut(s)	134, 149, 681, 1396, 1473, 1848
AfiI	CCNNNNNNNGG	1 cut(s)	367
AflIII	ACRYGT	2 cut(s)	129, 433
AgsI	TTSAA	4 cut(s)	1093, 1211, 1295, 1646
AhdI	GACNNNNNGTC	1 cut(s)	903
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	2 cut(s)	360, 1549
AjuI	GAANNNNNNNTTGG	2 cut(s)	1250, 1282
AleI	CACNNNNGTG	2 cut(s)	339, 871
AluBI	AGCT	8 cut(s)	166, 373, 686, 850, 1124, 1141, 1478, 1655
AluI	AGCT	8 cut(s)	166, 373, 686, 850, 1124, 1141, 1478, 1655
Alw21I	GWGCWC	4 cut(s)	288, 375, 394, 891
Alw26I	GTCTC	4 cut(s)	26, 122, 200, 1140
AlwI	GGATC	3 cut(s)	318, 1022, 1343
Ama87I	CYCGRG	2 cut(s)	70, 393
Aor13HI	TCCGGA	1 cut(s)	120
AoxI	GGCC	8 cut(s)	63, 240, 724, 1113, 1166, 1547, 1741, 1867
ApeKI	GCWGC	4 cut(s)	440, 542, 641, 1809
ApoI	RAATTY	3 cut(s)	76, 1338, 1762
AspLEI	GCGC	5 cut(s)	232, 247, 606, 1492, 1530
AspS9I	GGNCC	3 cut(s)	240, 1547, 1584
AsuC2I	CCSGG	1 cut(s)	1032
AsuHPI	GGTGA	3 cut(s)	112, 409, 1715
AsuII	TTCGAA	2 cut(s)	482, 1766
AsuNHI	GCTAGC	1 cut(s)	1120
AvaI	CYCGRG	2 cut(s)	70, 393
AvaII	GGWCC	1 cut(s)	1584
AxyI	CCTNAGG	1 cut(s)	1852
BalI	TGGCCA	1 cut(s)	1743
BanI	GGYRCC	1 cut(s)	603
BanII	GRGCYC	1 cut(s)	375
BauI	CACGAG	1 cut(s)	1656
BbsI	GAAGAC	1 cut(s)	566
Bbv12I	GWGCWC	4 cut(s)	288, 375, 394, 891
BbvI	GCAGC	4 cut(s)	452, 529, 628, 1821
BccI	CCATC	5 cut(s)	206, 512, 700, 812, 1369
BceAI	ACGGC	4 cut(s)	153, 159, 166, 342
BciT130I	CCWGG	2 cut(s)	362, 1551
BciVI	GTATCC	1 cut(s)	1249
BclI	TGATCA	2 cut(s)	1576, 1611
BcnI	CCSGG	1 cut(s)	1032
BcoDI	GTCTC	4 cut(s)	26, 122, 200, 1140
BfaI	CTAG	4 cut(s)	833, 851, 983, 1121
BfmI	CTRYAG	2 cut(s)	901, 990
BfoI	RGCGCY	1 cut(s)	607
BfuAI	ACCTGC	1 cut(s)	1900
BfuI	GTATCC	1 cut(s)	1249
Bme1390I	CCNGG	3 cut(s)	362, 1032, 1551
Bme18I	GGWCC	1 cut(s)	1584
BmeRI	GACNNNNNGTC	1 cut(s)	903
BmeT110I	CYCGRG	2 cut(s)	70, 393
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	3 cut(s)	240, 1547, 1584
BmiI	GGNNCC	3 cut(s)	605, 924, 1585
BmrFI	CCNGG	3 cut(s)	362, 1032, 1551
BmrI	ACTGGG	1 cut(s)	547
BmsI	GCATC	2 cut(s)	580, 1781
BmtI	GCTAGC	1 cut(s)	1124
BmuI	ACTGGG	1 cut(s)	547
BpiI	GAAGAC	1 cut(s)	566
BplI	GAGNNNNNCTC	2 cut(s)	179, 211
BpmI	CTGGAG	3 cut(s)	218, 358, 1338
Bpu14I	TTCGAA	2 cut(s)	482, 1766
BpuMI	CCSGG	1 cut(s)	1032
BsaHI	GRCGYC	1 cut(s)	604
BsaI	GGTCTC	3 cut(s)	26, 200, 1140
BsaJI	CCNNGG	8 cut(s)	69, 405, 781, 1232, 1550, 1813, 1822, 1870
BsaWI	WCCGGW	1 cut(s)	120
BsaXI	ACNNNNNCTCC	4 cut(s)	282, 312, 1314, 1344
Bsc4I	CCNNNNNNNGG	1 cut(s)	367
Bse118I	RCCGGY	1 cut(s)	947
Bse1I	ACTGG	7 cut(s)	235, 341, 542, 727, 1136, 1321, 1675
Bse21I	CCTNAGG	1 cut(s)	1852
Bse3DI	GCAATG	5 cut(s)	329, 1225, 1530, 1744, 1804
BseAI	TCCGGA	1 cut(s)	120
BseBI	CCWGG	2 cut(s)	362, 1551
BseDI	CCNNGG	8 cut(s)	69, 405, 781, 1232, 1550, 1813, 1822, 1870
BseGI	GGATG	2 cut(s)	595, 1038
BseLI	CCNNNNNNNGG	1 cut(s)	367
BseMI	GCAATG	5 cut(s)	329, 1225, 1530, 1744, 1804
BseMII	CTCAG	5 cut(s)	284, 966, 1151, 1369, 1614
BseNI	ACTGG	7 cut(s)	235, 341, 542, 727, 1136, 1321, 1675
BseRI	GAGGAG	6 cut(s)	41, 44, 125, 306, 618, 1335
BseX3I	CGGCCG	1 cut(s)	63
BseXI	GCAGC	4 cut(s)	452, 529, 628, 1821
BseYI	CCCAGC	2 cut(s)	686, 1895
BsgI	GTGCAG	1 cut(s)	1761
Bsh1236I	CGCG	3 cut(s)	230, 245, 1492
Bsh1285I	CGRYCG	1 cut(s)	66
BshFI	GGCC	8 cut(s)	65, 242, 726, 1115, 1168, 1549, 1743, 1869
BshNI	GGYRCC	1 cut(s)	603
BsiEI	CGRYCG	1 cut(s)	66
BsiHKAI	GWGCWC	4 cut(s)	288, 375, 394, 891
BsiHKCI	CYCGRG	2 cut(s)	70, 393
BsiSI	CCGG	4 cut(s)	121, 948, 1032, 1112
BsiWI	CGTACG	1 cut(s)	147
BslFI	GGGAC	2 cut(s)	1597, 1681
BslI	CCNNNNNNNGG	1 cut(s)	367
BsmAI	GTCTC	4 cut(s)	26, 122, 200, 1140
BsmFI	GGGAC	2 cut(s)	1597, 1681
BsmI	GAATGC	5 cut(s)	307, 406, 496, 793, 1774
BsnI	GGCC	8 cut(s)	65, 242, 726, 1115, 1168, 1549, 1743, 1869
Bso31I	GGTCTC	3 cut(s)	26, 200, 1140
BsoBI	CYCGRG	2 cut(s)	70, 393
Bsp119I	TTCGAA	2 cut(s)	482, 1766
Bsp1286I	GDGCHC	4 cut(s)	288, 375, 394, 891
Bsp13I	TCCGGA	1 cut(s)	120
Bsp1407I	TGTACA	2 cut(s)	132, 1471
Bsp143I	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
Bsp19I	CCATGG	2 cut(s)	1813, 1822
BspANI	GGCC	8 cut(s)	65, 242, 726, 1115, 1168, 1549, 1743, 1869
BspCNI	CTCAG	5 cut(s)	283, 965, 1150, 1368, 1613
BspEI	TCCGGA	1 cut(s)	120
BspFNI	CGCG	3 cut(s)	230, 245, 1492
BspLI	GGNNCC	3 cut(s)	605, 924, 1585
BspMAI	CTGCAG	1 cut(s)	994
BspMI	ACCTGC	1 cut(s)	1900
BspOI	GCTAGC	1 cut(s)	1124
BspPI	GGATC	3 cut(s)	318, 1022, 1343
BspT104I	TTCGAA	2 cut(s)	482, 1766
BspT107I	GGYRCC	1 cut(s)	603
BspTNI	GGTCTC	3 cut(s)	26, 200, 1140
BsrBI	CCGCTC	1 cut(s)	269
BsrDI	GCAATG	5 cut(s)	329, 1225, 1530, 1744, 1804
BsrFI	RCCGGY	1 cut(s)	947
BsrGI	TGTACA	2 cut(s)	132, 1471
BsrI	ACTGG	7 cut(s)	235, 341, 542, 727, 1136, 1321, 1675
BssAI	RCCGGY	1 cut(s)	947
BssECI	CCNNGG	8 cut(s)	69, 405, 781, 1232, 1550, 1813, 1822, 1870
BssMI	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
BssNAI	GTATAC	1 cut(s)	569
BssNI	GRCGYC	1 cut(s)	604
BssSI	CACGAG	1 cut(s)	1656
BssT1I	CCWWGG	5 cut(s)	781, 1232, 1813, 1822, 1870
Bst1107I	GTATAC	1 cut(s)	569
Bst2BI	CACGAG	1 cut(s)	1656
Bst2UI	CCWGG	2 cut(s)	362, 1551
Bst4CI	ACNGT	5 cut(s)	452, 673, 1202, 1330, 1468
Bst6I	CTCTTC	2 cut(s)	1176, 1448
BstACI	GRCGYC	1 cut(s)	604
BstAUI	TGTACA	2 cut(s)	132, 1471
BstBI	TTCGAA	2 cut(s)	482, 1766
BstC8I	GCNNGC	3 cut(s)	667, 949, 1122
BstDEI	CTNAG	8 cut(s)	270, 952, 1137, 1355, 1541, 1600, 1852, 1902
BstDSI	CCRYGG	2 cut(s)	1813, 1822
BstF5I	GGATG	2 cut(s)	595, 1038
BstFNI	CGCG	3 cut(s)	230, 245, 1492
BstH2I	RGCGCY	1 cut(s)	607
BstHHI	GCGC	5 cut(s)	232, 247, 606, 1492, 1530
BstKTI	GATC	8 cut(s)	15, 46, 313, 648, 1030, 1351, 1579, 1614
BstMAI	GTCTC	4 cut(s)	26, 122, 200, 1140
BstMBI	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	4 cut(s)	65, 743, 1121, 1815
BstNI	CCWGG	2 cut(s)	362, 1551
BstNSI	RCATGY	2 cut(s)	437, 669
BstSCI	CCNGG	3 cut(s)	360, 1030, 1549
BstSFI	CTRYAG	2 cut(s)	901, 990
BstUI	CGCG	3 cut(s)	230, 245, 1492
BstV1I	GCAGC	4 cut(s)	452, 529, 628, 1821
BstV2I	GAAGAC	1 cut(s)	566
BstX2I	RGATCY	1 cut(s)	1027
BstXI	CCANNNNNNTGG	2 cut(s)	870, 1733
BstYI	RGATCY	1 cut(s)	1027
BstZ17I	GTATAC	1 cut(s)	569
BstZI	CGGCCG	1 cut(s)	63
Bsu36I	CCTNAGG	1 cut(s)	1852
BsuI	GTATCC	1 cut(s)	1249
BsuRI	GGCC	8 cut(s)	65, 242, 726, 1115, 1168, 1549, 1743, 1869
BtgI	CCRYGG	2 cut(s)	1813, 1822
BtgZI	GCGATG	1 cut(s)	1319
BtrI	CACGTC	1 cut(s)	130
BtsCI	GGATG	2 cut(s)	595, 1038
BtsIMutI	CAGTG	4 cut(s)	242, 334, 535, 1326
BveI	ACCTGC	1 cut(s)	1900
Cac8I	GCNNGC	3 cut(s)	667, 949, 1122
CciNI	GCGGCCGC	1 cut(s)	63
CfoI	GCGC	5 cut(s)	232, 247, 606, 1492, 1530
Cfr10I	RCCGGY	1 cut(s)	947
Cfr13I	GGNCC	3 cut(s)	240, 1547, 1584
Csp6I	GTAC	6 cut(s)	133, 148, 680, 1395, 1472, 1847
CviQI	GTAC	6 cut(s)	133, 148, 680, 1395, 1472, 1847
DdeI	CTNAG	8 cut(s)	270, 952, 1137, 1355, 1541, 1600, 1852, 1902
DinI	GGCGCC	1 cut(s)	605
DpnI	GATC	8 cut(s)	14, 45, 312, 647, 1029, 1350, 1578, 1613
DpnII	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
DriI	GACNNNNNGTC	1 cut(s)	903
EaeI	YGGCCR	2 cut(s)	63, 1741
EagI	CGGCCG	1 cut(s)	63
Eam1104I	CTCTTC	2 cut(s)	1176, 1448
Eam1105I	GACNNNNNGTC	1 cut(s)	903
EarI	CTCTTC	2 cut(s)	1176, 1448
EciI	GGCGGA	1 cut(s)	99
Ecl136II	GAGCTC	1 cut(s)	373
EclXI	CGGCCG	1 cut(s)	63
Eco130I	CCWWGG	5 cut(s)	781, 1232, 1813, 1822, 1870
Eco147I	AGGCCT	1 cut(s)	1168
Eco24I	GRGCYC	1 cut(s)	375
Eco31I	GGTCTC	3 cut(s)	26, 200, 1140
Eco47I	GGWCC	1 cut(s)	1584
Eco52I	CGGCCG	1 cut(s)	63
Eco53kI	GAGCTC	1 cut(s)	373
Eco57I	CTGAAG	2 cut(s)	152, 1546
Eco81I	CCTNAGG	1 cut(s)	1852
Eco88I	CYCGRG	2 cut(s)	70, 393
EcoICRI	GAGCTC	1 cut(s)	373
EcoRII	CCWGG	2 cut(s)	360, 1549
EcoT14I	CCWWGG	5 cut(s)	781, 1232, 1813, 1822, 1870
EcoT22I	ATGCAT	2 cut(s)	595, 1774
EcoT38I	GRGCYC	1 cut(s)	375
EgeI	GGCGCC	1 cut(s)	605
EheI	GGCGCC	1 cut(s)	605
ErhI	CCWWGG	5 cut(s)	781, 1232, 1813, 1822, 1870
FalI	AAGNNNNNCTT	4 cut(s)	514, 546, 686, 718
FaqI	GGGAC	2 cut(s)	1597, 1681
FauI	CCCGC	3 cut(s)	44, 310, 342
FauNDI	CATATG	1 cut(s)	1573
FbaI	TGATCA	2 cut(s)	1576, 1611
FblI	GTMKAC	1 cut(s)	568
FokI	GGATG	2 cut(s)	602, 1025
FriOI	GRGCYC	1 cut(s)	375
FspBI	CTAG	4 cut(s)	833, 851, 983, 1121
GlaI	GCGC	5 cut(s)	231, 246, 605, 1491, 1529
GsaI	CCCAGC	2 cut(s)	690, 1899
GsuI	CTGGAG	3 cut(s)	218, 358, 1338
HaeII	RGCGCY	1 cut(s)	607
HaeIII	GGCC	8 cut(s)	65, 242, 726, 1115, 1168, 1549, 1743, 1869
HapII	CCGG	4 cut(s)	121, 948, 1032, 1112
HhaI	GCGC	5 cut(s)	232, 247, 606, 1492, 1530
Hin1I	GRCGYC	1 cut(s)	604
Hin6I	GCGC	5 cut(s)	230, 245, 604, 1490, 1528
HinP1I	GCGC	5 cut(s)	230, 245, 604, 1490, 1528
HincII	GTYRAC	3 cut(s)	839, 942, 1311
HindII	GTYRAC	3 cut(s)	839, 942, 1311
HindIII	AAGCTT	2 cut(s)	164, 1476
HinfI	GANTC	6 cut(s)	50, 115, 1058, 1323, 1649, 1712
HpaI	GTTAAC	1 cut(s)	942
HpaII	CCGG	4 cut(s)	121, 948, 1032, 1112
HphI	GGTGA	3 cut(s)	112, 409, 1715
Hpy166II	GTNNAC	7 cut(s)	133, 135, 569, 839, 942, 1311, 1829
Hpy188III	TCNNGA	9 cut(s)	47, 72, 121, 509, 587, 1292, 1609, 1658, 1887
Hpy8I	GTNNAC	7 cut(s)	133, 135, 569, 839, 942, 1311, 1829
Hpy99I	CGWCG	5 cut(s)	10, 26, 58, 131, 145
HpyAV	CCTTC	2 cut(s)	301, 789
HpyCH4III	ACNGT	5 cut(s)	452, 673, 1202, 1330, 1468
HpyCH4IV	ACGT	4 cut(s)	129, 571, 811, 1758
HpyF10VI	GCNNNNNNNGC	4 cut(s)	65, 743, 1121, 1815
HpyF3I	CTNAG	8 cut(s)	270, 952, 1137, 1355, 1541, 1600, 1852, 1902
HpySE526I	ACGT	4 cut(s)	129, 571, 811, 1758
Hsp92I	GRCGYC	1 cut(s)	604
HspAI	GCGC	5 cut(s)	230, 245, 604, 1490, 1528
KasI	GGCGCC	1 cut(s)	603
Kpn2I	TCCGGA	1 cut(s)	120
KroI	GCCGGC	1 cut(s)	947
KroNI	GCCGGC	1 cut(s)	949
Ksp22I	TGATCA	2 cut(s)	1576, 1611
KspAI	GTTAAC	1 cut(s)	942
Kzo9I	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
LmnI	GCTCC	5 cut(s)	237, 370, 894, 928, 1373
Lsp1109I	GCAGC	4 cut(s)	452, 529, 628, 1821
LweI	GCATC	2 cut(s)	580, 1781
MaeI	CTAG	4 cut(s)	833, 851, 983, 1121
MaeII	ACGT	4 cut(s)	129, 571, 811, 1758
MaeIII	GTNAC	9 cut(s)	653, 709, 895, 1034, 1127, 1196, 1324, 1330, 1556
MalI	GATC	8 cut(s)	14, 45, 312, 647, 1029, 1350, 1578, 1613
MbiI	CCGCTC	1 cut(s)	269
MboI	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
MfeI	CAATTG	1 cut(s)	1243
MflI	RGATCY	1 cut(s)	1027
MhlI	GDGCHC	4 cut(s)	288, 375, 394, 891
MlsI	TGGCCA	1 cut(s)	1743
MluCI	AATT	9 cut(s)	76, 1214, 1243, 1265, 1338, 1485, 1637, 1690, 1762
MluNI	TGGCCA	1 cut(s)	1743
Mly113I	GGCGCC	1 cut(s)	604
MlyI	GAGTC	2 cut(s)	124, 1332
Mox20I	TGGCCA	1 cut(s)	1743
Mph1103I	ATGCAT	2 cut(s)	595, 1774
MroI	TCCGGA	1 cut(s)	120
MroNI	GCCGGC	1 cut(s)	947
MscI	TGGCCA	1 cut(s)	1743
MseI	TTAA	3 cut(s)	249, 941, 1383
MslI	CAYNNNNRTG	5 cut(s)	339, 871, 1043, 1407, 1731
Msp20I	TGGCCA	1 cut(s)	1743
MspI	CCGG	4 cut(s)	121, 948, 1032, 1112
MspR9I	CCNGG	3 cut(s)	362, 1032, 1551
MunI	CAATTG	1 cut(s)	1243
Mva1269I	GAATGC	5 cut(s)	307, 406, 496, 793, 1774
MvaI	CCWGG	2 cut(s)	362, 1551
MvnI	CGCG	3 cut(s)	230, 245, 1492
MwoI	GCNNNNNNNGC	4 cut(s)	65, 743, 1121, 1815
NaeI	GCCGGC	1 cut(s)	949
NarI	GGCGCC	1 cut(s)	604
NciI	CCSGG	1 cut(s)	1032
NcoI	CCATGG	2 cut(s)	1813, 1822
NdeI	CATATG	1 cut(s)	1573
NdeII	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
NgoMIV	GCCGGC	1 cut(s)	947
NheI	GCTAGC	1 cut(s)	1120
NlaIV	GGNNCC	3 cut(s)	605, 924, 1585
NmeAIII	GCCGAG	1 cut(s)	430
NmuCI	GTSAC	4 cut(s)	895, 1196, 1324, 1556
NotI	GCGGCCGC	1 cut(s)	63
NsiI	ATGCAT	2 cut(s)	595, 1774
NspI	RCATGY	2 cut(s)	437, 669
NspV	TTCGAA	2 cut(s)	482, 1766
OliI	CACNNNNGTG	2 cut(s)	339, 871
PaeI	GCATGC	1 cut(s)	669
PceI	AGGCCT	1 cut(s)	1168
PciI	ACATGT	1 cut(s)	433
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 143
PctI	GAATGC	5 cut(s)	307, 406, 496, 793, 1774
PdiI	GCCGGC	1 cut(s)	949
PfeI	GAWTC	4 cut(s)	50, 1058, 1649, 1712
Pfl23II	CGTACG	1 cut(s)	147
PleI	GAGTC	2 cut(s)	123, 1331
PluTI	GGCGCC	1 cut(s)	607
PpsI	GAGTC	2 cut(s)	123, 1331
PscI	ACATGT	1 cut(s)	433
PsiI	TTATAA	1 cut(s)	549
Psp124BI	GAGCTC	1 cut(s)	375
Psp1406I	AACGTT	1 cut(s)	811
Psp6I	CCWGG	2 cut(s)	360, 1549
PspFI	CCCAGC	2 cut(s)	686, 1895
PspGI	CCWGG	2 cut(s)	360, 1549
PspLI	CGTACG	1 cut(s)	147
PspN4I	GGNNCC	3 cut(s)	605, 924, 1585
PspPI	GGNCC	3 cut(s)	240, 1547, 1584
PsrI	GAACNNNNNNTAC	2 cut(s)	116, 148
PstI	CTGCAG	1 cut(s)	994
PsuI	RGATCY	1 cut(s)	1027
RsaI	GTAC	6 cut(s)	134, 149, 681, 1396, 1473, 1848
RsaNI	GTAC	6 cut(s)	133, 148, 680, 1395, 1472, 1847
RseI	CAYNNNNRTG	5 cut(s)	339, 871, 1043, 1407, 1731
SacI	GAGCTC	1 cut(s)	375
SaqAI	TTAA	3 cut(s)	249, 941, 1383
Sau3AI	GATC	8 cut(s)	12, 43, 310, 645, 1027, 1348, 1576, 1611
Sau96I	GGNCC	3 cut(s)	240, 1547, 1584
SchI	GAGTC	2 cut(s)	124, 1332
ScrFI	CCNGG	3 cut(s)	362, 1032, 1551
SduI	GDGCHC	4 cut(s)	288, 375, 394, 891
SfaNI	GCATC	2 cut(s)	580, 1781
SfcI	CTRYAG	2 cut(s)	901, 990
SfoI	GGCGCC	1 cut(s)	605
SfuI	TTCGAA	2 cut(s)	482, 1766
SinI	GGWCC	1 cut(s)	1584
SmiMI	CAYNNNNRTG	5 cut(s)	339, 871, 1043, 1407, 1731
SphI	GCATGC	1 cut(s)	669
Sse9I	AATT	9 cut(s)	76, 1214, 1243, 1265, 1338, 1485, 1637, 1690, 1762
SseBI	AGGCCT	1 cut(s)	1168
SspDI	GGCGCC	1 cut(s)	603
SspI	AATATT	1 cut(s)	1498
SspMI	CTAG	4 cut(s)	833, 851, 983, 1121
SstI	GAGCTC	1 cut(s)	375
StuI	AGGCCT	1 cut(s)	1168
StyD4I	CCNGG	3 cut(s)	360, 1030, 1549
StyI	CCWWGG	5 cut(s)	781, 1232, 1813, 1822, 1870
TaaI	ACNGT	5 cut(s)	452, 673, 1202, 1330, 1468
TaiI	ACGT	4 cut(s)	132, 574, 814, 1761
TaqI	TCGA	5 cut(s)	15, 24, 482, 793, 1766
TaqII	GACCGA	1 cut(s)	224
TasI	AATT	9 cut(s)	76, 1214, 1243, 1265, 1338, 1485, 1637, 1690, 1762
TatI	WGTACW	2 cut(s)	132, 1471
TauI	GCSGC	6 cut(s)	62, 65, 68, 245, 266, 269
TfiI	GAWTC	4 cut(s)	50, 1058, 1649, 1712
Tru1I	TTAA	3 cut(s)	249, 941, 1383
Tru9I	TTAA	3 cut(s)	249, 941, 1383
TscAI	CASTG	4 cut(s)	242, 341, 542, 1333
TseFI	GTSAC	4 cut(s)	895, 1196, 1324, 1556
TseI	GCWGC	4 cut(s)	440, 542, 641, 1809
Tsp45I	GTSAC	4 cut(s)	895, 1196, 1324, 1556
TspDTI	ATGAA	7 cut(s)	1061, 1085, 1095, 1356, 1524, 1641, 1784
TspGWI	ACGGA	2 cut(s)	303, 1891
TspRI	CASTG	4 cut(s)	242, 341, 542, 1333
VpaK11BI	GGWCC	1 cut(s)	1584
XapI	RAATTY	3 cut(s)	76, 1338, 1762
XceI	RCATGY	2 cut(s)	437, 669
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1811, 1820
XmiI	GTMKAC	1 cut(s)	568
XspI	CTAG	4 cut(s)	833, 851, 983, 1121
Zsp2I	ATGCAT	2 cut(s)	595, 1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.