AT5G20720

Belongs to the GroES chaperonin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013355
Physical Location & Seq
Forward (+)
7014662 .. 7016698
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20720.4

Sequence Viewer

Length: 762 bp
ATGGCGGCGACTCAACTTACAGCGTCACCAGTGACTATGTCAGCAAGGAGCTTAGCCTCGCTGGATGGTCTCAGAGCTTCGAGTGTCAAGTTTTCATCTTTGAAACCAGGGACCCTTAGACAGAGCCAGTTCCGTCGTTTGGTTGTCAAAGCTGCTTCTGTTGTTGCCCCTAAGTATACTTCAATTAAGCCATTGGGAGATCGAGTTTTGGTGAAGATCAAGGAGGCAGAGGAGAAGACTTTAGGTGGTATCTTACTTCCATCCACTGCTCAATCAAAACCTCAAGGAGGTGAAGTCGTTGCCGTGGGTGAAGGAAGAACTATTGGGAAGAACAAAATTGATATCACTGTCCCTACTGGAGCACAAATTATCTACTCCAAATACGCAGGAACTGAGGTGGAGTTCAATGATGTGAAGCATCTTATCCTCAAGGAAGATGATATTGTTGGCATTCTTGAGACAGAGGACATCAAAGATCTCAAACCTTTGAATGACCGAGTCTTTATTAAGGTTGCTGAGGCGGAGGAGAAAACAGCTGGAGGGTTGTTGTTAACCGAGACTACCAAAGAGAAGCCTTCTATTGGCACGGTGATAGCAGTTGGACCGGGTTCCCTAGACGAGGAAGGTAAAATTACGCCTCTACCAGTATCAACCGGAAGCACAGTACTTTACTCCAAGTATGCTGGTAACGACTTCAAGGGCAAAGATGGTTCCAACTACATTGCCCTCAGAGCTTCAGATGTGATGGCTATACTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005507 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006457 GO:0006458 GO:0006950 GO:0006986 GO:0008150 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009787 GO:0009788 GO:0009893 GO:0009941 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010646 GO:0010648 GO:0016020 GO:0016043 GO:0019222 GO:0022607 GO:0023051 GO:0023057 GO:0031323 GO:0031325 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0035966 GO:0042221 GO:0042651 GO:0043085 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051082 GO:0051084 GO:0051085 GO:0051087 GO:0051259 GO:0051262 GO:0051290 GO:0051291 GO:0051341 GO:0051353 GO:0055035 GO:0061077 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080134 GO:0080135 GO:0090322 GO:1900407 GO:1900409 GO:1901031 GO:1901033 GO:1901419 GO:1901420 GO:1901668 GO:1901671 GO:1902882 GO:1902884 GO:1904833 GO:1905957 GO:1905958 GO:2000121 GO:2000377 GO:2000379
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cpn10

Protein Analysis

253

Amino Acids

26.8

Weight (kDa)

8.86

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn10 PF00166 62 - 151 4.3e-30 Chaperonin 10 Kd subunit
Cpn10 PF00166 160 - 251 4.2e-28 Chaperonin 10 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013355)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20720 AT5G20720 AT5G20720 AT5G20720
fragaria_vesca FvH4_1g09160
malus_domestica MD02G1096600.v1.1 MD15G1221300.v1.1
prunus_persica Prupe.7G194300_v2.0.a1
pyrus_communis pycom02g07660
rosa_chinensis RchiOBHm_Chr2g0095561
rosa_multiflora Rmu_sc0005382.1_g000006 Rmu_sc0038699.1_g000001
rosa_roxburghii Rroxscaffold_2G00146190
rosa_rugosa Rorug02G0052400
rosa_samantha Rh2BG101700 Rh2CG103800 Rh2DG101100
rosa_wichuraiana Rw2G007610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 176
AciI CCGC 2 cut(s) 5, 521
AcuI CTGAAG 1 cut(s) 720
AfaI GTAC 1 cut(s) 666
AfiI CCNNNNNNNGG 4 cut(s) 139, 287, 581, 619
AgsI TTSAA 5 cut(s) 103, 183, 406, 490, 697
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 5 cut(s) 51, 77, 152, 536, 734
AluI AGCT 5 cut(s) 51, 77, 152, 536, 734
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 3 cut(s) 74, 452, 551
AlwNI CAGNNNCTG 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 111, 602
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 5 cut(s) 18, 223, 302, 320, 601
AvaII GGWCC 2 cut(s) 111, 602
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 364
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 139
BccI CCATC 4 cut(s) 59, 268, 701, 739
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 108
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 3 cut(s) 74, 452, 551
BfaI CTAG 1 cut(s) 614
BglII AGATCT 1 cut(s) 475
BisI GCNGC 2 cut(s) 6, 153
BlpI GCTNAGC 1 cut(s) 52
BlsI GCNGC 2 cut(s) 7, 154
BmcAI AGTACT 1 cut(s) 666
Bme1390I CCNGG 2 cut(s) 108, 606
Bme18I GGWCC 2 cut(s) 111, 602
BmgT120I GGNCC 2 cut(s) 111, 602
BmiI GGNNCC 4 cut(s) 112, 113, 610, 712
BmrFI CCNGG 2 cut(s) 108, 606
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 2 cut(s) 378, 558
Bpu10I CCTNAGC 1 cut(s) 516
Bpu1102I GCTNAGC 1 cut(s) 52
BpuEI CTTGAG 3 cut(s) 267, 413, 476
BpuMI CCSGG 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 107, 303
BsaWI WCCGGW 1 cut(s) 653
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 279, 309
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 287, 581, 619
Bse1I ACTGG 4 cut(s) 29, 127, 361, 644
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 107, 303
BseGI GGATG 2 cut(s) 70, 260
BseLI CCNNNNNNNGG 4 cut(s) 139, 287, 581, 619
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 4 cut(s) 85, 384, 507, 742
BseNI ACTGG 4 cut(s) 29, 127, 361, 644
BseRI GAGGAG 2 cut(s) 245, 539
BseXI GCAGC 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 2 cut(s) 605, 654
BslFI GGGAC 2 cut(s) 124, 335
BslI CCNNNNNNNGG 4 cut(s) 139, 287, 581, 619
BsmAI GTCTC 3 cut(s) 74, 452, 551
BsmFI GGGAC 2 cut(s) 124, 335
BsmI GAATGC 1 cut(s) 450
Bso31I GGTCTC 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 199, 216, 475
Bsp1720I GCTNAGC 1 cut(s) 52
BspACI CCGC 2 cut(s) 5, 521
BspCNI CTCAG 4 cut(s) 84, 385, 508, 741
BspLI GGNNCC 4 cut(s) 112, 113, 610, 712
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 4 cut(s) 29, 127, 361, 644
BssECI CCNNGG 2 cut(s) 107, 303
BssMI GATC 3 cut(s) 199, 216, 475
BssNAI GTATAC 1 cut(s) 177
Bst1107I GTATAC 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 349, 589, 664
BstDEI CTNAG 8 cut(s) 52, 71, 116, 171, 393, 516, 728, 759
BstDSI CCRYGG 1 cut(s) 303
BstENI CCTNNNNNAGG 2 cut(s) 285, 617
BstF5I GGATG 2 cut(s) 70, 260
BstKTI GATC 3 cut(s) 202, 219, 478
BstMAI GTCTC 3 cut(s) 74, 452, 551
BstMBI GATC 3 cut(s) 199, 216, 475
BstMWI GCNNNNNNNGC 1 cut(s) 731
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 106, 604
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BstZ17I GTATAC 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 2 cut(s) 70, 260
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 3 cut(s) 36, 264, 345
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 111, 602
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 665
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 8 cut(s) 52, 71, 116, 171, 393, 516, 728, 759
DpnI GATC 3 cut(s) 201, 218, 477
DpnII GATC 3 cut(s) 199, 216, 475
EciI GGCGGA 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 111, 602
Eco57I CTGAAG 1 cut(s) 720
EcoNI CCTNNNNNAGG 2 cut(s) 285, 617
EcoO109I RGGNCCY 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 106
EcoRV GATATC 1 cut(s) 343
FaiI YATR 4 cut(s) 38, 177, 681, 752
FaqI GGGAC 2 cut(s) 124, 335
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 6, 153
FokI GGATG 2 cut(s) 77, 247
Fsp4HI GCNGC 2 cut(s) 6, 153
FspBI CTAG 1 cut(s) 614
GluI GCNGC 2 cut(s) 6, 153
GsuI CTGGAG 2 cut(s) 378, 558
HapII CCGG 2 cut(s) 605, 654
HgaI GACGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 2 cut(s) 10, 498
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 2 cut(s) 605, 654
HphI GGTGA 5 cut(s) 18, 223, 302, 320, 601
Hpy166II GTNNAC 2 cut(s) 177, 552
Hpy188I TCNGA 3 cut(s) 74, 731, 739
Hpy188III TCNNGA 1 cut(s) 455
Hpy8I GTNNAC 2 cut(s) 177, 552
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 3 cut(s) 305, 585, 617
HpyCH4III ACNGT 3 cut(s) 349, 589, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 731
HpyF3I CTNAG 8 cut(s) 52, 71, 116, 171, 393, 516, 728, 759
KflI GGGWCCC 1 cut(s) 111
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 3 cut(s) 199, 216, 475
LmnI GCTCC 2 cut(s) 48, 359
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 614
MaeIII GTNAC 3 cut(s) 24, 31, 686
MalI GATC 3 cut(s) 201, 218, 477
MboI GATC 3 cut(s) 199, 216, 475
MboII GAAGA 5 cut(s) 226, 247, 327, 340, 446
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 4 cut(s) 183, 336, 366, 630
MlyI GAGTC 2 cut(s) 4, 507
MmeI TCCRAC 2 cut(s) 580, 738
MseI TTAA 3 cut(s) 186, 507, 551
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 2 cut(s) 605, 654
MspR9I CCNGG 2 cut(s) 108, 606
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 731
NciI CCSGG 1 cut(s) 606
NdeII GATC 3 cut(s) 199, 216, 475
NlaIV GGNNCC 4 cut(s) 112, 113, 610, 712
NmuCI GTSAC 2 cut(s) 24, 31
PctI GAATGC 1 cut(s) 450
PflFI GACNNNGTC 2 cut(s) 37, 497
PkrI GCNGC 2 cut(s) 7, 154
PleI GAGTC 2 cut(s) 4, 506
PpsI GAGTC 2 cut(s) 4, 506
PpuMI RGGWCCY 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 4 cut(s) 112, 113, 610, 712
PspPI GGNCC 2 cut(s) 111, 602
PspPPI RGGWCCY 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 475
PsyI GACNNNGTC 2 cut(s) 37, 497
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
SaqAI TTAA 3 cut(s) 186, 507, 551
SatI GCNGC 2 cut(s) 6, 153
Sau3AI GATC 3 cut(s) 199, 216, 475
Sau96I GGNCC 2 cut(s) 111, 602
ScaI AGTACT 1 cut(s) 666
SchI GAGTC 2 cut(s) 4, 507
ScrFI CCNGG 2 cut(s) 108, 606
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 1 cut(s) 427
SinI GGWCC 2 cut(s) 111, 602
SmlI CTYRAG 3 cut(s) 282, 428, 455
SmoI CTYRAG 3 cut(s) 282, 428, 455
Sse9I AATT 4 cut(s) 183, 336, 366, 630
SsiI CCGC 2 cut(s) 5, 521
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 106, 604
TaaI ACNGT 3 cut(s) 349, 589, 664
TaqI TCGA 2 cut(s) 80, 202
TaqII GACCGA 1 cut(s) 510
TasI AATT 4 cut(s) 183, 336, 366, 630
TatI WGTACW 1 cut(s) 664
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 186, 507, 551
Tru9I TTAA 3 cut(s) 186, 507, 551
TscAI CASTG 3 cut(s) 36, 271, 352
TseFI GTSAC 2 cut(s) 24, 31
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 24, 31
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 3 cut(s) 36, 271, 352
Tth111I GACNNNGTC 2 cut(s) 37, 497
VpaK11BI GGWCC 2 cut(s) 111, 602
XagI CCTNNNNNAGG 2 cut(s) 285, 617
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 1 cut(s) 614
ZrmI AGTACT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.