MD02G1096600.v1.1

Belongs to the GroES chaperonin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013355
Physical Location & Seq
Forward (+)
7630517 .. 7632518
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1096600.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCAGCAGCTCAGCTGACCGCATCGTCGATCTCGGCCAGGAGTTTGGCTTCGTTTGAAGGGCTCAGACCTTCAAACCTCAAGTTCGGGTCTCCGGCATGTGTCTCACTTGTCAACCACCGCTCCTTCCGTGGCCTCGCTGTCAAGGCCGCCACCACCGTCGCTCCCAAGTACACTTCAATTAAGCCTTTGGGCGACAGAGTGCTTGTGAAGATCAAGACTGTGGAAGAGAAGACCGGCGGTGGAATTTTACTTCCAACCACAGCTCAGACAAAGCCTCAAGGAGGTGAAGTTGTTGCTGTTGGAGAGGGTAACACAATTGGGAAGACAAAAGTGGGCATCAGTGTGGAGACAGGTGCTGAAGTTGTATACTCTAAGTATGCTGGGACAGAGGTGGAGTTCAATGGTGCGAAGCATCTTATCCTGAAGGATGATGACATAGTCGGTATTCTTGAAACCGAAGATGTGAAGGATCTCAAGCCTTTGAATGATAGAGTTCTAATCAAGGTTGCCGAGGCGGAGTTAAAGACTGCCGGAGGTTTGTTGCTGACAGAGGCTAGCAAGGAGAAGCCCTCCATTGGCACGGTGATTGCCGTCGGACCCGGTACTCTTGATGAGGAAGGAAACAGGAAACCACTATCCATATCCCAGGGAAGCACAGTTTTGTACTCCAAGTATGCAGGGAATGACTTCAAGGGCAAAGACGGTACTGAGTACATAGCCTTGAGAGCTTCGGATGTCATCGCCATTCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005507 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006457 GO:0006458 GO:0006950 GO:0006986 GO:0008150 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009787 GO:0009788 GO:0009893 GO:0009941 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010646 GO:0010648 GO:0016020 GO:0016043 GO:0019222 GO:0022607 GO:0023051 GO:0023057 GO:0031323 GO:0031325 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0035966 GO:0042221 GO:0042651 GO:0043085 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051082 GO:0051084 GO:0051085 GO:0051087 GO:0051259 GO:0051262 GO:0051290 GO:0051291 GO:0051341 GO:0051353 GO:0055035 GO:0061077 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080134 GO:0080135 GO:0090322 GO:1900407 GO:1900409 GO:1901031 GO:1901033 GO:1901419 GO:1901420 GO:1901668 GO:1901671 GO:1902882 GO:1902884 GO:1904833 GO:1905957 GO:1905958 GO:2000121 GO:2000377 GO:2000379
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cpn10

Protein Analysis

253

Amino Acids

26.31

Weight (kDa)

8.46

Isoelectric Point (pI)

22.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn10 PF00166 61 - 150 1.9e-28 Chaperonin 10 Kd subunit
Cpn10 PF00166 159 - 250 3.2e-28 Chaperonin 10 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013355)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20720 AT5G20720 AT5G20720 AT5G20720
fragaria_vesca FvH4_1g09160
malus_domestica MD02G1096600.v1.1 MD15G1221300.v1.1
prunus_persica Prupe.7G194300_v2.0.a1
pyrus_communis pycom02g07660
rosa_chinensis RchiOBHm_Chr2g0095561
rosa_multiflora Rmu_sc0005382.1_g000006 Rmu_sc0038699.1_g000001
rosa_roxburghii Rroxscaffold_2G00146190
rosa_rugosa Rorug02G0052400
rosa_samantha Rh2BG101700 Rh2CG103800 Rh2DG101100
rosa_wichuraiana Rw2G007610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 369
AciI CCGC 5 cut(s) 21, 121, 150, 240, 518
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 381, 446
AfaI GTAC 5 cut(s) 173, 607, 668, 709, 716
AfiI CCNNNNNNNGG 2 cut(s) 284, 578
AgsI TTSAA 7 cut(s) 59, 75, 180, 403, 455, 487, 694
AjnI CCWGG 2 cut(s) 38, 648
AluBI AGCT 4 cut(s) 11, 16, 266, 731
AluI AGCT 4 cut(s) 11, 16, 266, 731
Alw26I GTCTC 3 cut(s) 96, 109, 344
AlwI GGATC 1 cut(s) 480
AlwNI CAGNNNCTG 1 cut(s) 359
AoxI GGCC 3 cut(s) 36, 133, 147
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 689
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 2 cut(s) 299, 598
AsuNHI GCTAGC 1 cut(s) 557
AvaII GGWCC 1 cut(s) 599
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 2 cut(s) 239, 332
BbvI GCAGC 2 cut(s) 17, 20
BceAI ACGGC 1 cut(s) 578
BciT130I CCWGG 2 cut(s) 40, 650
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 3 cut(s) 96, 109, 344
BfaI CTAG 1 cut(s) 558
BisI GCNGC 3 cut(s) 6, 9, 150
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 3 cut(s) 7, 10, 151
Bme1390I CCNGG 3 cut(s) 40, 603, 650
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 3 cut(s) 40, 603, 650
BmsI GCATC 3 cut(s) 32, 348, 424
BmtI GCTAGC 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 239, 332
BplI GAGNNNNNCTC 2 cut(s) 557, 589
Bpu1102I GCTNAGC 1 cut(s) 12
BpuEI CTTGAG 4 cut(s) 65, 264, 461, 745
BpuMI CCSGG 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 130, 513, 648, 649
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 148, 178, 276, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 578
Bse118I RCCGGY 1 cut(s) 236
BseBI CCWGG 2 cut(s) 40, 650
BseDI CCNNGG 4 cut(s) 130, 513, 648, 649
BseGI GGATG 2 cut(s) 436, 742
BseLI CCNNNNNNNGG 2 cut(s) 284, 578
BseMII CTCAG 4 cut(s) 26, 79, 281, 702
BseXI GCAGC 2 cut(s) 17, 20
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 3 cut(s) 38, 135, 149
BsiSI CCGG 4 cut(s) 95, 237, 534, 603
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 284, 578
BsmAI GTCTC 3 cut(s) 96, 109, 344
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 3 cut(s) 38, 135, 149
Bso31I GGTCTC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 3 cut(s) 30, 213, 472
Bsp1720I GCTNAGC 1 cut(s) 12
BspACI CCGC 5 cut(s) 21, 121, 150, 240, 518
BspANI GGCC 3 cut(s) 38, 135, 149
BspCNI CTCAG 4 cut(s) 25, 78, 280, 703
BspLI GGNNCC 1 cut(s) 601
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 480
BspTNI GGTCTC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 4 cut(s) 130, 513, 648, 649
BssMI GATC 3 cut(s) 30, 213, 472
BssNAI GTATAC 1 cut(s) 370
Bst1107I GTATAC 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 40, 650
Bst4CI ACNGT 5 cut(s) 160, 223, 586, 661, 707
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 559
BstDEI CTNAG 6 cut(s) 12, 65, 267, 375, 711, 756
BstDSI CCRYGG 1 cut(s) 130
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 436, 742
BstKTI GATC 3 cut(s) 33, 216, 475
BstMAI GTCTC 3 cut(s) 96, 109, 344
BstMBI GATC 3 cut(s) 30, 213, 472
BstMWI GCNNNNNNNGC 2 cut(s) 146, 728
BstNI CCWGG 2 cut(s) 40, 650
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 38, 601, 648
BstV1I GCAGC 2 cut(s) 17, 20
BstV2I GAAGAC 2 cut(s) 239, 332
BstX2I RGATCY 1 cut(s) 472
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 472
BstZ17I GTATAC 1 cut(s) 370
BsuRI GGCC 3 cut(s) 38, 135, 149
BtgI CCRYGG 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 727
BtsCI GGATG 2 cut(s) 436, 742
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 559
CaiI CAGNNNCTG 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 5 cut(s) 172, 606, 667, 708, 715
CviAII CATG 1 cut(s) 99
CviQI GTAC 5 cut(s) 172, 606, 667, 708, 715
DdeI CTNAG 6 cut(s) 12, 65, 267, 375, 711, 756
DpnI GATC 3 cut(s) 32, 215, 474
DpnII GATC 3 cut(s) 30, 213, 472
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 381, 446
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 38, 648
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 102
FaiI YATR 7 cut(s) 100, 370, 381, 440, 644, 678, 719
FaqI GGGAC 1 cut(s) 400
FatI CATG 1 cut(s) 98
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 6, 9, 150
FokI GGATG 2 cut(s) 443, 749
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 3 cut(s) 6, 9, 150
FspBI CTAG 1 cut(s) 558
GluI GCNGC 3 cut(s) 6, 9, 150
GsaI CCCAGC 1 cut(s) 387
HaeIII GGCC 3 cut(s) 38, 135, 149
HapII CCGG 4 cut(s) 95, 237, 534, 603
Hin1II CATG 1 cut(s) 102
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HpaII CCGG 4 cut(s) 95, 237, 534, 603
HphI GGTGA 2 cut(s) 299, 598
Hpy166II GTNNAC 3 cut(s) 115, 174, 370
Hpy188I TCNGA 4 cut(s) 68, 270, 599, 736
Hpy188III TCNNGA 4 cut(s) 217, 424, 452, 611
Hpy8I GTNNAC 3 cut(s) 115, 174, 370
Hpy99I CGWCG 3 cut(s) 31, 164, 599
HpyAV CCTTC 6 cut(s) 53, 81, 136, 421, 463, 614
HpyCH4III ACNGT 5 cut(s) 160, 223, 586, 661, 707
HpyCH4V TGCA 1 cut(s) 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 728
HpyF3I CTNAG 6 cut(s) 12, 65, 267, 375, 711, 756
Hsp92II CATG 1 cut(s) 102
Kzo9I GATC 3 cut(s) 30, 213, 472
LmnI GCTCC 2 cut(s) 128, 169
Lsp1109I GCAGC 2 cut(s) 17, 20
LweI GCATC 3 cut(s) 32, 348, 424
MaeI CTAG 1 cut(s) 558
MaeIII GTNAC 1 cut(s) 311
MalI GATC 3 cut(s) 32, 215, 474
MbiI CCGCTC 1 cut(s) 123
MboI GATC 3 cut(s) 30, 213, 472
MboII GAAGA 5 cut(s) 223, 239, 244, 337, 473
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 3 cut(s) 180, 246, 318
MmeI TCCRAC 3 cut(s) 281, 283, 577
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 2 cut(s) 183, 524
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 4 cut(s) 95, 237, 534, 603
MspR9I CCNGG 3 cut(s) 40, 603, 650
MunI CAATTG 1 cut(s) 318
MvaI CCWGG 2 cut(s) 40, 650
MwoI GCNNNNNNNGC 2 cut(s) 146, 728
NciI CCSGG 1 cut(s) 603
NdeII GATC 3 cut(s) 30, 213, 472
NheI GCTAGC 1 cut(s) 557
NlaIII CATG 1 cut(s) 102
NlaIV GGNNCC 1 cut(s) 601
NmeAIII GCCGAG 2 cut(s) 14, 538
NspI RCATGY 1 cut(s) 102
PasI CCCWGGG 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 689
PflFI GACNNNGTC 1 cut(s) 440
PkrI GCNGC 3 cut(s) 7, 10, 151
Psp6I CCWGG 2 cut(s) 38, 648
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 38, 648
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 472
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 5 cut(s) 173, 607, 668, 709, 716
RsaNI GTAC 5 cut(s) 172, 606, 667, 708, 715
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 2 cut(s) 183, 524
SatI GCNGC 3 cut(s) 6, 9, 150
Sau3AI GATC 3 cut(s) 30, 213, 472
Sau96I GGNCC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 40, 603, 650
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 32, 348, 424
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 4 cut(s) 80, 279, 476, 724
SmoI CTYRAG 4 cut(s) 80, 279, 476, 724
Sse9I AATT 3 cut(s) 180, 246, 318
SsiI CCGC 5 cut(s) 21, 121, 150, 240, 518
SspMI CTAG 1 cut(s) 558
StyD4I CCNGG 3 cut(s) 38, 601, 648
TaaI ACNGT 5 cut(s) 160, 223, 586, 661, 707
TaqI TCGA 1 cut(s) 29
TasI AATT 3 cut(s) 180, 246, 318
TatI WGTACW 3 cut(s) 171, 666, 714
TauI GCSGC 1 cut(s) 152
Tru1I TTAA 2 cut(s) 183, 524
Tru9I TTAA 2 cut(s) 183, 524
TscAI CASTG 1 cut(s) 349
TseI GCWGC 2 cut(s) 5, 8
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 1 cut(s) 349
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 599
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.