AT5G21060

homoserine dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008338
Physical Location & Seq
Reverse (-)
7148978 .. 7152922
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G21060.2

Sequence Viewer

Length: 1131 bp
ATGAAGAAGATCCCGGTGCTTCTCATGGGCTGCGGAGGCGTTGGCCGCCACCTTCTTCAACACATCGTCTCTTGCCGATCGCTTCACGCCAAAATGGGAGTTCATATACGGGTGATTGGAGTATGTGATAGTAAATCCTTAGTTGCTCCAATGGATGTGCTCAAAGAGGAACTGAATGATGAACTCTTGTCAGAAGTTTGCCTTATCAAATCAACTGGTTCTGCATTATCGAAGTTGGGTGCTTTGGGTGGATATCGAGTCGTCCATGATTCCGAGTTAAGCACGGAAACAGAAGAGATTGCAAAACTTCTTGGTAAATCTACAGGCTTAGCTGTGGTGGATTGCTCAGCCAGCATGGAGACGATTGAAATATTGATGAAGGCAGTTGACTTGGGTTGCTGTATTGTCCTGGCAAATAAGAAGCCTGTTACTTCAACGCTGGAACATTACGACAAATTGGCTTTGCATCCGCGATTCATCCGCCATGAATCTACTGTTGGTGCTGGTCTTCCTGTCATCGCATCCTTGAACCGCATAATTTCGTCTGGAGATCCTGTTCATAGGATTGTCGGTAGTTTGAGCGGTACCTTGGGATATGTAATGAGTGAGCTTGAAGATGGCAAGCCTCTAAGCCAAGTTGTTCAAGCTGCCAAAAAGCTGGGGTACACTGAACCAGATCCACGTGACGACCTCGGTGGCATGGATGTTGCTAGAAAGGGTTTGATTCTTGCTCGACTTCTTGGGAAGAGGATAATTATGGACAGCATTAAGATAGAGAGCTTGTATCCAGAAGAGATGGGACCTGGACTCATGTCTGTGGATGATTTCCTCCATAATGGAATAGTAAAACTTGATCAGAATATAGAGGAGAGAGTTAAAAAAGCTTCATCAAAAGGGTGTGTGCTTCGTTATGTCTGTGTGATTGAGGGTTCAAGCGTTCAAGTTGGCATTCGAGAAGTTTCAAAAGATTCACCATTGGGACGGCTAAGAGGCAGTGACAATATAGTAGAGATATATAGCCGTTGCTACAAGGAGCAGCCTTTGGTGATTCAAGGTGCCGGAGCCGGGAACGACACAACTGCAGCCGGTGTTCTTGCAGACATCATCGACTTGCAGGATCTTTTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homoserine_dh NAD_binding_3

Protein Analysis

376

Amino Acids

40.41

Weight (kDa)

6.28

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homoserine_dh PF00742 173 - 371 2.3e-48 Homoserine dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 584
AccB1I GGYRCC 2 cut(s) 584, 1055
AccBSI CCGCTC 1 cut(s) 582
AccII CGCG 1 cut(s) 472
AciI CCGC 6 cut(s) 33, 46, 470, 481, 532, 582
AclWI GGATC 4 cut(s) 4, 545, 671, 1125
AcoI YGGCCR 1 cut(s) 43
AcvI CACGTG 1 cut(s) 683
AfaI GTAC 2 cut(s) 586, 665
AfiI CCNNNNNNNGG 1 cut(s) 1065
AjnI CCWGG 2 cut(s) 408, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
AloI GAACNNNNNNTCC 2 cut(s) 540, 572
AluBI AGCT 6 cut(s) 332, 610, 647, 658, 780, 884
AluI AGCT 6 cut(s) 332, 610, 647, 658, 780, 884
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 73, 353
AlwI GGATC 4 cut(s) 4, 545, 671, 1125
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 4 cut(s) 30, 647, 1036, 1082
Asp718I GGTACC 1 cut(s) 584
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 2 cut(s) 14, 1066
AsuHPI GGTGA 3 cut(s) 124, 963, 1057
AvaII GGWCC 1 cut(s) 800
BanI GGYRCC 2 cut(s) 584, 1055
BbrPI CACGTG 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 17, 634, 1048, 1094
BccI CCATC 2 cut(s) 611, 790
BceAI ACGGC 2 cut(s) 998, 1005
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 2 cut(s) 410, 804
BciVI GTATCC 1 cut(s) 795
BclI TGATCA 1 cut(s) 853
BcnI CCSGG 2 cut(s) 14, 1066
BcoDI GTCTC 2 cut(s) 73, 353
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 321, 1080
BfuI GTATCC 1 cut(s) 795
BisI GCNGC 5 cut(s) 31, 46, 648, 1037, 1083
BlpI GCTNAGC 2 cut(s) 328, 346
BlsI GCNGC 5 cut(s) 32, 47, 649, 1038, 1084
Bme1390I CCNGG 4 cut(s) 14, 410, 804, 1066
Bme18I GGWCC 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 4 cut(s) 586, 801, 1057, 1063
BmrFI CCNGG 4 cut(s) 14, 410, 804, 1066
BmsI GCATC 2 cut(s) 475, 530
BoxI GACNNNNGTC 1 cut(s) 811
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 567
Bpu1102I GCTNAGC 2 cut(s) 328, 346
BpuMI CCSGG 2 cut(s) 14, 1066
BsaAI YACGTR 1 cut(s) 683
BsaJI CCNNGG 2 cut(s) 588, 691
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 1065
Bse118I RCCGGY 1 cut(s) 1085
Bse1I ACTGG 1 cut(s) 220
BseBI CCWGG 2 cut(s) 410, 804
BseDI CCNNGG 2 cut(s) 588, 691
BseGI GGATG 6 cut(s) 160, 466, 477, 521, 709, 826
BseLI CCNNNNNNNGG 1 cut(s) 1065
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 4 cut(s) 17, 634, 1048, 1094
BseYI CCCAGC 1 cut(s) 658
Bsh1236I CGCG 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 45
BshNI GGYRCC 2 cut(s) 584, 1055
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 4 cut(s) 14, 1059, 1065, 1086
BslFI GGGAC 2 cut(s) 813, 993
BslI CCNNNNNNNGG 1 cut(s) 1065
BsmAI GTCTC 2 cut(s) 73, 353
BsmBI CGTCTC 2 cut(s) 73, 353
BsmFI GGGAC 2 cut(s) 813, 993
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
Bsp1720I GCTNAGC 2 cut(s) 328, 346
BspACI CCGC 6 cut(s) 33, 46, 470, 481, 532, 582
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 359
BspFNI CGCG 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 586, 801, 1057, 1063
BspMAI CTGCAG 1 cut(s) 1084
BspPI GGATC 4 cut(s) 4, 545, 671, 1125
BspT107I GGYRCC 2 cut(s) 584, 1055
BsrBI CCGCTC 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 1085
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 1085
BssECI CCNNGG 2 cut(s) 588, 691
BssMI GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
BssT1I CCWWGG 1 cut(s) 588
Bst2UI CCWGG 2 cut(s) 410, 804
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 3 cut(s) 288, 740, 786
BstBAI YACGTR 1 cut(s) 683
BstC8I GCNNGC 2 cut(s) 352, 623
BstDEI CTNAG 5 cut(s) 139, 328, 346, 629, 986
BstF5I GGATG 6 cut(s) 160, 466, 477, 521, 709, 826
BstFNI CGCG 1 cut(s) 472
BstKTI GATC 6 cut(s) 12, 80, 553, 679, 856, 1120
BstMAI GTCTC 2 cut(s) 73, 353
BstMBI GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 3 cut(s) 36, 45, 351
BstNI CCWGG 2 cut(s) 410, 804
BstPAI GACNNNNGTC 1 cut(s) 811
BstSCI CCNGG 4 cut(s) 12, 408, 802, 1064
BstSFI CTRYAG 2 cut(s) 321, 1080
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 4 cut(s) 17, 634, 1048, 1094
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 4 cut(s) 9, 550, 676, 1117
BstXI CCANNNNNNTGG 1 cut(s) 658
BstYI RGATCY 4 cut(s) 9, 550, 676, 1117
BsuI GTATCC 1 cut(s) 795
BsuRI GGCC 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 502
BtsCI GGATG 6 cut(s) 160, 466, 477, 521, 709, 826
BtsI GCAGTG 1 cut(s) 1000
BtsIMutI CAGTG 3 cut(s) 666, 1000, 1126
Cac8I GCNNGC 2 cut(s) 352, 623
Cfr10I RCCGGY 1 cut(s) 1085
Cfr13I GGNCC 1 cut(s) 800
Csp6I GTAC 2 cut(s) 585, 664
CviAII CATG 6 cut(s) 25, 266, 355, 485, 700, 811
CviQI GTAC 2 cut(s) 585, 664
DdeI CTNAG 5 cut(s) 139, 328, 346, 629, 986
DpnI GATC 6 cut(s) 11, 79, 552, 678, 855, 1119
DpnII GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 3 cut(s) 288, 740, 786
EarI CTCTTC 3 cut(s) 288, 740, 786
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 588
Eco32I GATATC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 800
Eco72I CACGTG 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 800
EcoRII CCWGG 2 cut(s) 408, 802
EcoRV GATATC 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 588
Esp3I CGTCTC 2 cut(s) 73, 353
FaeI CATG 6 cut(s) 28, 269, 358, 488, 703, 814
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FaqI GGGAC 2 cut(s) 813, 993
FatI CATG 6 cut(s) 24, 265, 354, 484, 699, 810
FbaI TGATCA 1 cut(s) 853
Fnu4HI GCNGC 5 cut(s) 31, 46, 648, 1037, 1083
FokI GGATG 6 cut(s) 167, 453, 464, 508, 716, 833
Fsp4HI GCNGC 5 cut(s) 31, 46, 648, 1037, 1083
FspBI CTAG 1 cut(s) 711
GluI GCNGC 5 cut(s) 31, 46, 648, 1037, 1083
GsaI CCCAGC 1 cut(s) 662
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 1 cut(s) 45
HapII CCGG 4 cut(s) 14, 1059, 1065, 1086
Hin1II CATG 6 cut(s) 28, 269, 358, 488, 703, 814
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 8 cut(s) 258, 269, 474, 488, 724, 807, 968, 1048
HpaII CCGG 4 cut(s) 14, 1059, 1065, 1086
HphI GGTGA 3 cut(s) 124, 963, 1057
Hpy166II GTNNAC 2 cut(s) 388, 666
Hpy188I TCNGA 3 cut(s) 193, 274, 858
Hpy188III TCNNGA 3 cut(s) 546, 788, 953
Hpy8I GTNNAC 2 cut(s) 388, 666
HpyAV CCTTC 2 cut(s) 62, 373
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 1 cut(s) 682
HpyCH4V TGCA 6 cut(s) 224, 302, 466, 1082, 1097, 1114
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 45, 351
HpyF3I CTNAG 5 cut(s) 139, 328, 346, 629, 986
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 6 cut(s) 28, 269, 358, 488, 703, 814
KpnI GGTACC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
LmnI GCTCC 3 cut(s) 151, 1033, 1061
Lsp1109I GCAGC 4 cut(s) 17, 634, 1048, 1094
LweI GCATC 2 cut(s) 475, 530
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 682
MaeIII GTNAC 3 cut(s) 427, 683, 995
MalI GATC 6 cut(s) 11, 79, 552, 678, 855, 1119
MbiI CCGCTC 1 cut(s) 582
MboI GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
MboII GAAGA 8 cut(s) 16, 19, 47, 305, 500, 626, 757, 803
MflI RGATCY 4 cut(s) 9, 550, 676, 1117
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 3 cut(s) 455, 537, 753
MlyI GAGTC 2 cut(s) 267, 801
MnlI CCTC 9 cut(s) 29, 160, 636, 701, 741, 839, 859, 919, 983
MseI TTAA 3 cut(s) 278, 768, 876
MslI CAYNNNNRTG 1 cut(s) 815
MspI CCGG 4 cut(s) 14, 1059, 1065, 1086
MspR9I CCNGG 4 cut(s) 14, 410, 804, 1066
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 2 cut(s) 410, 804
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 3 cut(s) 36, 45, 351
NciI CCSGG 2 cut(s) 14, 1066
NdeII GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
NlaIII CATG 6 cut(s) 28, 269, 358, 488, 703, 814
NlaIV GGNNCC 4 cut(s) 586, 801, 1057, 1063
NmuCI GTSAC 2 cut(s) 683, 995
PctI GAATGC 1 cut(s) 948
PfeI GAWTC 6 cut(s) 269, 474, 488, 724, 968, 1048
PkrI GCNGC 5 cut(s) 32, 47, 649, 1038, 1084
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 2 cut(s) 266, 801
PmaCI CACGTG 1 cut(s) 683
PmlI CACGTG 1 cut(s) 683
PpsI GAGTC 2 cut(s) 266, 801
Ppu21I YACGTR 1 cut(s) 683
PpuMI RGGWCCY 1 cut(s) 800
PshAI GACNNNNGTC 1 cut(s) 811
Psp5II RGGWCCY 1 cut(s) 800
Psp6I CCWGG 2 cut(s) 408, 802
PspCI CACGTG 1 cut(s) 683
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 2 cut(s) 408, 802
PspN4I GGNNCC 4 cut(s) 586, 801, 1057, 1063
PspPI GGNCC 1 cut(s) 800
PspPPI RGGWCCY 1 cut(s) 800
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 4 cut(s) 9, 550, 676, 1117
PvuI CGATCG 1 cut(s) 80
RsaI GTAC 2 cut(s) 586, 665
RsaNI GTAC 2 cut(s) 585, 664
RseI CAYNNNNRTG 1 cut(s) 815
SaqAI TTAA 3 cut(s) 278, 768, 876
SatI GCNGC 5 cut(s) 31, 46, 648, 1037, 1083
Sau3AI GATC 6 cut(s) 9, 77, 550, 676, 853, 1117
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 2 cut(s) 267, 801
ScrFI CCNGG 4 cut(s) 14, 410, 804, 1066
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 2 cut(s) 475, 530
SfcI CTRYAG 2 cut(s) 321, 1080
SinI GGWCC 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 815
Sse9I AATT 3 cut(s) 455, 537, 753
SsiI CCGC 6 cut(s) 33, 46, 470, 481, 532, 582
SspI AATATT 1 cut(s) 372
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 12, 408, 802, 1064
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 1 cut(s) 685
TaqI TCGA 5 cut(s) 230, 256, 733, 952, 1107
TasI AATT 3 cut(s) 455, 537, 753
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 6 cut(s) 269, 474, 488, 724, 968, 1048
Tru1I TTAA 3 cut(s) 278, 768, 876
Tru9I TTAA 3 cut(s) 278, 768, 876
TscAI CASTG 2 cut(s) 673, 1000
TseFI GTSAC 2 cut(s) 683, 995
TseI GCWGC 4 cut(s) 30, 647, 1036, 1082
Tsp45I GTSAC 2 cut(s) 683, 995
TspDTI ATGAA 8 cut(s) 17, 92, 195, 392, 466, 501, 548, 876
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 673, 1000
VpaK11BI GGWCC 1 cut(s) 800
XspI CTAG 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.