Rw5G030830

homoserine dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008338
Physical Location & Seq
Forward (+)
49269530 .. 49276524
6995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030830.1

Sequence Viewer

Length: 1107 bp
ATGAAGAAGGTGATCTCCCTAATTCTGATGGGTTGCGGAGGCGTTGGCCGTCAGCTCCTCCAACACATCGTCTCCTGCCGACCCCTTCACGCCAAGCAGGGAATCCACCTGAGAGTTGTGGGAGTATCTGACAGTAAATCCTTAGTTGTTGCGCCTGACGTATCGACTGTGGAGTTGGCTGATAATTCTTTATTGCAACTTTGCCGGATCAAGTCCGATGGTTCTTCTCTGCATACGCTTGCCGCCTCTGGTCAAGTAGAGGTATTCACCGATTCTGAATCAACCAAAAAGATTATCGATGTTGCTGCACTCTTGGGTAAATCAACAGGTTTGGCTCTTGTAGATTGCTCTGCCAGTTCTGAGACTATTCCACTGCTCACCCAAGCGGTTGATTTGGGATGTTGCATTGTCATGGCAAACAAGAAGCCTCTTACTTCAACAATGGAGGATTATGACAAACTAATTTCACAGCCACGTCACATACGTCATGAGTCAACTGTTGGTGCCGGCCTTCCTGTCATATCATCCTTGCATCGCATTCTTTCATCTGGAGATCCTGTCCGCCGCATTATTGGGAGTTTGAGTGGTGAAGATGGAAAGCCAATTAGCGAAGTTGTGCGATCTGCTAAAAATCTGGGTTACACTGAACCAGATCCACGTGATGACCTCAGTGGGATGGATGTTGCAAGAAAGGCTTTGATACTTGCTAGGCTTCTCGGTTGGCGTATTAATTTGGATGACATTAAGATTGAGAGCTTGTATCCTAAAGAAATGGCACCCGGTGTAATGTCTGTGGAAGAATTTCTGGGAACTGGGCTACTATTGCTTGATAAAGATATCACAGAGAGAGTCTCCAAAGCTTCTTTGAGTGGGAATGTGCTACGTTATGTCTGTGTGATTGAGGCCTCAAGGTGTGAAGTTGGCATTCAAGAGCTTGCAAAGGATTCTCCTTTAGGAAGACTGAGAGGAAGTGACAATGTGCTGGAGATTTACAGTAGATGTTATAATGACCAACCACTGGTCATTCAAGGAGCTGGAGCTGGCAATGATACTACTGCTGCTGGAGTCCTTGCTGATATCATTGATATTCAAGATTTATTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homoserine_dh NAD_binding_3

Protein Analysis

368

Amino Acids

39.34

Weight (kDa)

5.71

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homoserine_dh PF00742 172 - 361 1.4e-38 Homoserine dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
AccB1I GGYRCC 2 cut(s) 503, 775
AccB7I CCANNNNNTGG 1 cut(s) 1018
AciI CCGC 5 cut(s) 36, 243, 386, 562, 565
AclWI GGATC 3 cut(s) 215, 548, 647
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 800
AcvI CACGTG 1 cut(s) 659
AdeI CACNNNGTG 1 cut(s) 782
AfiI CCNNNNNNNGG 1 cut(s) 1018
AgsI TTSAA 4 cut(s) 438, 929, 1028, 1091
AjiI CACGTC 1 cut(s) 476
AluBI AGCT 6 cut(s) 55, 756, 860, 934, 1034, 1040
AluI AGCT 6 cut(s) 55, 756, 860, 934, 1034, 1040
Alw26I GTCTC 3 cut(s) 76, 356, 856
AlwI GGATC 3 cut(s) 215, 548, 647
AoxI GGCC 3 cut(s) 46, 508, 903
ApeKI GCWGC 2 cut(s) 305, 1058
ApoI RAATTY 1 cut(s) 800
AseI ATTAAT 1 cut(s) 729
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 780
AsuHPI GGTGA 4 cut(s) 22, 259, 370, 599
BanI GGYRCC 2 cut(s) 503, 775
BbrPI CACGTG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 964
BbvI GCAGC 2 cut(s) 292, 1045
BccI CCATC 4 cut(s) 22, 212, 587, 670
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciVI GTATCC 1 cut(s) 771
BcnI CCSGG 1 cut(s) 780
BcoDI GTCTC 3 cut(s) 76, 356, 856
BfaI CTAG 1 cut(s) 708
BfuI GTATCC 1 cut(s) 771
BisI GCNGC 4 cut(s) 243, 306, 565, 1059
BlsI GCNGC 4 cut(s) 244, 307, 566, 1060
Bme1390I CCNGG 1 cut(s) 780
BmgBI CACGTC 1 cut(s) 476
BmiI GGNNCC 2 cut(s) 505, 777
BmrFI CCNGG 1 cut(s) 780
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 964
BplI GAGNNNNNCTC 2 cut(s) 836, 868
BpmI CTGGAG 4 cut(s) 570, 1004, 1056, 1083
BpuEI CTTGAG 1 cut(s) 892
BpuMI CCSGG 1 cut(s) 780
Bsa29I ATCGAT 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 659
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 543, 568, 573, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 1018
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 3 cut(s) 354, 817, 1023
Bse3DI GCAATG 1 cut(s) 1051
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 5 cut(s) 404, 524, 681, 685, 742
BseLI CCNNNNNNNGG 1 cut(s) 1018
BseMI GCAATG 1 cut(s) 1051
BseMII CTCAG 4 cut(s) 101, 351, 682, 953
BseNI ACTGG 3 cut(s) 354, 817, 1023
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 292, 1045
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 3 cut(s) 48, 510, 905
BshNI GGYRCC 2 cut(s) 503, 775
BshVI ATCGAT 1 cut(s) 297
BsiSI CCGG 3 cut(s) 205, 507, 780
BslI CCNNNNNNNGG 1 cut(s) 1018
BsmAI GTCTC 3 cut(s) 76, 356, 856
BsmBI CGTCTC 1 cut(s) 76
BsmI GAATGC 2 cut(s) 537, 924
BsnI GGCC 3 cut(s) 48, 510, 905
Bsp143I GATC 5 cut(s) 12, 207, 553, 620, 652
BspACI CCGC 5 cut(s) 36, 243, 386, 562, 565
BspANI GGCC 3 cut(s) 48, 510, 905
BspCNI CTCAG 4 cut(s) 102, 352, 681, 954
BspDI ATCGAT 1 cut(s) 297
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 505, 777
BspPI GGATC 3 cut(s) 215, 548, 647
BspT107I GGYRCC 2 cut(s) 503, 775
BsrDI GCAATG 1 cut(s) 1051
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 3 cut(s) 354, 817, 1023
BssAI RCCGGY 1 cut(s) 506
BssMI GATC 5 cut(s) 12, 207, 553, 620, 652
Bst4CI ACNGT 4 cut(s) 134, 169, 499, 995
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 240, 508, 936, 1042
BstDEI CTNAG 5 cut(s) 110, 142, 360, 668, 962
BstF5I GGATG 5 cut(s) 404, 524, 681, 685, 742
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 5 cut(s) 15, 210, 556, 623, 655
BstMAI GTCTC 3 cut(s) 76, 356, 856
BstMBI GATC 5 cut(s) 12, 207, 553, 620, 652
BstMWI GCNNNNNNNGC 2 cut(s) 692, 823
BstSCI CCNGG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 292, 1045
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 2 cut(s) 553, 652
BstYI RGATCY 2 cut(s) 553, 652
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 3 cut(s) 48, 510, 905
BsuTUI ATCGAT 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 518
BtrI CACGTC 1 cut(s) 476
BtsCI GGATG 5 cut(s) 404, 524, 681, 685, 742
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 4 cut(s) 371, 642, 676, 1016
Cac8I GCNNGC 4 cut(s) 240, 508, 936, 1042
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 506
ClaI ATCGAT 1 cut(s) 297
CviAII CATG 2 cut(s) 412, 488
DdeI CTNAG 5 cut(s) 110, 142, 360, 668, 962
DpnI GATC 5 cut(s) 14, 209, 555, 622, 654
DpnII GATC 5 cut(s) 12, 207, 553, 620, 652
DraIII CACNNNGTG 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 46
EciI GGCGGA 1 cut(s) 551
Eco147I AGGCCT 1 cut(s) 905
Eco32I GATATC 2 cut(s) 838, 1078
Eco72I CACGTG 1 cut(s) 659
EcoRV GATATC 2 cut(s) 838, 1078
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 415, 491
FaiI YATR 8 cut(s) 234, 413, 453, 482, 489, 521, 888, 1005
FalI AAGNNNNNCTT 2 cut(s) 679, 711
FatI CATG 2 cut(s) 411, 487
Fnu4HI GCNGC 4 cut(s) 243, 306, 565, 1059
FokI GGATG 5 cut(s) 411, 511, 688, 692, 749
Fsp4HI GCNGC 4 cut(s) 243, 306, 565, 1059
FspBI CTAG 1 cut(s) 708
GlaI GCGC 1 cut(s) 153
GluI GCNGC 4 cut(s) 243, 306, 565, 1059
GsuI CTGGAG 4 cut(s) 570, 1004, 1056, 1083
HaeIII GGCC 3 cut(s) 48, 510, 905
HapII CCGG 3 cut(s) 205, 507, 780
HhaI GCGC 1 cut(s) 154
Hin1II CATG 2 cut(s) 415, 491
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 7 cut(s) 102, 272, 278, 491, 849, 944, 1065
HpaII CCGG 3 cut(s) 205, 507, 780
HphI GGTGA 4 cut(s) 22, 259, 370, 599
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 5 cut(s) 27, 130, 217, 277, 361
Hpy188III TCNNGA 4 cut(s) 488, 549, 929, 1091
Hpy8I GTNNAC 1 cut(s) 495
HpyAV CCTTC 2 cut(s) 95, 521
HpyCH4III ACNGT 4 cut(s) 134, 169, 499, 995
HpyCH4IV ACGT 5 cut(s) 159, 475, 484, 658, 883
HpyCH4V TGCA 7 cut(s) 196, 232, 308, 405, 532, 686, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 823
HpyF3I CTNAG 5 cut(s) 110, 142, 360, 668, 962
HpySE526I ACGT 5 cut(s) 159, 475, 484, 658, 883
Hsp92II CATG 2 cut(s) 415, 491
HspAI GCGC 1 cut(s) 152
KroI GCCGGC 1 cut(s) 506
KroNI GCCGGC 1 cut(s) 508
Kzo9I GATC 5 cut(s) 12, 207, 553, 620, 652
LmnI GCTCC 3 cut(s) 60, 1031, 1037
Lsp1109I GCAGC 2 cut(s) 292, 1045
LweI GCATC 1 cut(s) 541
MaeI CTAG 1 cut(s) 708
MaeII ACGT 5 cut(s) 159, 475, 484, 658, 883
MaeIII GTNAC 3 cut(s) 476, 638, 971
MalI GATC 5 cut(s) 14, 209, 555, 622, 654
MboI GATC 5 cut(s) 12, 207, 553, 620, 652
MboII GAAGA 5 cut(s) 16, 216, 602, 809, 969
MflI RGATCY 2 cut(s) 553, 652
MluCI AATT 6 cut(s) 21, 184, 462, 603, 730, 800
MlyI GAGTC 3 cut(s) 500, 858, 1074
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 506
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 2 cut(s) 729, 744
MslI CAYNNNNRTG 1 cut(s) 410
MspI CCGG 3 cut(s) 205, 507, 780
MspR9I CCNGG 1 cut(s) 780
Mva1269I GAATGC 2 cut(s) 537, 924
MwoI GCNNNNNNNGC 2 cut(s) 692, 823
NaeI GCCGGC 1 cut(s) 508
NciI CCSGG 1 cut(s) 780
NdeII GATC 5 cut(s) 12, 207, 553, 620, 652
NgoMIV GCCGGC 1 cut(s) 506
NlaIII CATG 2 cut(s) 415, 491
NlaIV GGNNCC 2 cut(s) 505, 777
NmuCI GTSAC 2 cut(s) 476, 971
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PctI GAATGC 2 cut(s) 537, 924
PdiI GCCGGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 4 cut(s) 102, 272, 278, 944
PflMI CCANNNNNTGG 1 cut(s) 1018
PkrI GCNGC 4 cut(s) 244, 307, 566, 1060
PleI GAGTC 3 cut(s) 499, 857, 1073
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 3 cut(s) 499, 857, 1073
Ppu21I YACGTR 1 cut(s) 659
PshBI ATTAAT 1 cut(s) 729
PsiI TTATAA 1 cut(s) 1005
PspCI CACGTG 1 cut(s) 659
PspN4I GGNNCC 2 cut(s) 505, 777
PsuI RGATCY 2 cut(s) 553, 652
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 2 cut(s) 729, 744
SatI GCNGC 4 cut(s) 243, 306, 565, 1059
Sau3AI GATC 5 cut(s) 12, 207, 553, 620, 652
SchI GAGTC 3 cut(s) 500, 858, 1074
ScrFI CCNGG 1 cut(s) 780
SfaNI GCATC 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
Sse9I AATT 6 cut(s) 21, 184, 462, 603, 730, 800
SseBI AGGCCT 1 cut(s) 905
SsiI CCGC 5 cut(s) 36, 243, 386, 562, 565
SspMI CTAG 1 cut(s) 708
StuI AGGCCT 1 cut(s) 905
StyD4I CCNGG 1 cut(s) 778
TaaI ACNGT 4 cut(s) 134, 169, 499, 995
TaiI ACGT 5 cut(s) 162, 478, 487, 661, 886
TaqI TCGA 2 cut(s) 164, 297
TasI AATT 6 cut(s) 21, 184, 462, 603, 730, 800
TauI GCSGC 2 cut(s) 245, 567
TfiI GAWTC 4 cut(s) 102, 272, 278, 944
Tru1I TTAA 2 cut(s) 729, 744
Tru9I TTAA 2 cut(s) 729, 744
TscAI CASTG 4 cut(s) 378, 649, 676, 1023
TseFI GTSAC 2 cut(s) 476, 971
TseI GCWGC 2 cut(s) 305, 1058
Tsp45I GTSAC 2 cut(s) 476, 971
TspDTI ATGAA 2 cut(s) 17, 534
TspRI CASTG 4 cut(s) 378, 649, 676, 1023
Van91I CCANNNNNTGG 1 cut(s) 1018
VspI ATTAAT 1 cut(s) 729
XapI RAATTY 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.