RchiOBHm_Chr5g0049661

homoserine dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008338
Physical Location & Seq
Forward (+)
47515133 .. 47522556
7424 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32729

Sequence Viewer

Length: 1134 bp
ATGAAGAAGGTGATCTCCCTAATTCTGATGGGCTGCGGAGGCGTTGGCCGCCAGCTCCTCCAACACATCGTCTCCTGCCGACCCCTTCACGCCAAGCAGGGAATCCACCTGAGAGTTGTGGGAGTATCTGACAGTAAATCCTTCGTTGTTGCGCCTGACGTGTCGACTATGGAGTTGGCTGATAATTCTTTATTGCAACTTTGCCGGATCAAGTCCGATGGTTCTTCTCTGCACACGCTTGCCGCCTCTGGTCAAGTAGAGGTATTCACCGATTCTGAATCAACCAAAAAGATTATCGATGTTGCTGCACTCTTGGGTAAATCAACAGGTTTGGCTCTTGTAGATTGCTCTGCGAGTTCTGAGACTATTCCACTGCTCACCCAAGCGGTTGATTTGGGATGTTGCATTGTCATGGCAAACAAGAAGCCTCTTACTTCAACAATGGAGGATTATGACAAACTAATTTCACACCCACGTCACATACGTCATGAGTCAACTGTTGGTGCCGGCCTTCCTGTCATATCATCCTTGCATCGCATTCTTTCATCTGGAGATCCTGTCTGCCGCATTATTGGGAGTTTGAGTGGAACATTGGGGTATGTTATGAGTGAGGTCGAAGATGGAAAGCCAATTAGCGAAGTTGTGCGATCTGCTAAAACTCTGGGTTACACTGAACCAGATCCACGTGATGACCTCAGTGGGATGGATGTTGCAAGAAAGGCTTTGATACTTGCTAGGCTTCTCGGTTGGCGTATTAATTTGGATGACATTAAGATTGAGTGCTTGTATCCTAAAGAAATGGCACCCGGTGTAATGTCTGTGGAAGAATTTCTGGGAACTGGGCTACTATTGCTTGATAAAGATATCAAAGAGAGAGTCTCCAAAGCTTCTTTGAGTGGGAATGTGCTACGTTATGTCTGTGTGATTGAGGCCTCAAGGTGTGAAGTTGGCATTCAAGAGCTTGCAAAGGATTCTCCTTTAGGAAGACTGAGAGGAAGTGACAATGTGCTGGAGATTTACAGTAGATGTTATAATGACCAACCACTGGTCATTCAAGGAGCTGGAGCTGGCAATGATACTACTGCTGCTGGAGTCCTTGCTGATATCATTGATATTCAAGATTTATTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homoserine_dh NAD_binding_3

Protein Analysis

377

Amino Acids

40.38

Weight (kDa)

5.67

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homoserine_dh PF00742 172 - 370 2e-46 Homoserine dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
AccB1I GGYRCC 2 cut(s) 503, 802
AccB7I CCANNNNNTGG 1 cut(s) 1045
AccI GTMKAC 1 cut(s) 164
AciI CCGC 5 cut(s) 36, 49, 243, 386, 565
AclWI GGATC 3 cut(s) 215, 548, 674
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 827
AcvI CACGTG 1 cut(s) 686
AdeI CACNNNGTG 1 cut(s) 809
AfiI CCNNNNNNNGG 1 cut(s) 1045
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 4 cut(s) 438, 956, 1055, 1118
AjiI CACGTC 2 cut(s) 160, 476
AluBI AGCT 5 cut(s) 55, 887, 961, 1061, 1067
AluI AGCT 5 cut(s) 55, 887, 961, 1061, 1067
Alw26I GTCTC 3 cut(s) 76, 356, 883
AlwI GGATC 3 cut(s) 215, 548, 674
AoxI GGCC 3 cut(s) 46, 508, 930
ApeKI GCWGC 3 cut(s) 33, 305, 1085
ApoI RAATTY 1 cut(s) 827
ArsI GACNNNNNNTTYG 2 cut(s) 861, 893
AseI ATTAAT 1 cut(s) 756
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 3 cut(s) 22, 259, 370
BanI GGYRCC 2 cut(s) 503, 802
BbrPI CACGTG 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 991
BbvI GCAGC 3 cut(s) 20, 292, 1072
BccI CCATC 4 cut(s) 22, 212, 614, 697
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciVI GTATCC 1 cut(s) 798
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 3 cut(s) 76, 356, 883
BfaI CTAG 1 cut(s) 735
BfuI GTATCC 1 cut(s) 798
BisI GCNGC 6 cut(s) 34, 49, 243, 306, 565, 1086
BlsI GCNGC 6 cut(s) 35, 50, 244, 307, 566, 1087
Bme1390I CCNGG 1 cut(s) 807
BmgBI CACGTC 2 cut(s) 160, 476
BmiI GGNNCC 2 cut(s) 505, 804
BmrFI CCNGG 1 cut(s) 807
BmrI ACTGGG 1 cut(s) 849
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 849
BpiI GAAGAC 1 cut(s) 991
BplI GAGNNNNNCTC 2 cut(s) 863, 895
BpmI CTGGAG 4 cut(s) 570, 1031, 1083, 1110
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 807
Bsa29I ATCGAT 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 686
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 543, 568, 573, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 1045
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 844, 1050
Bse3DI GCAATG 1 cut(s) 1078
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 5 cut(s) 404, 524, 708, 712, 769
BseLI CCNNNNNNNGG 1 cut(s) 1045
BseMI GCAATG 1 cut(s) 1078
BseMII CTCAG 4 cut(s) 101, 351, 709, 980
BseNI ACTGG 2 cut(s) 844, 1050
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 20, 292, 1072
BsgI GTGCAG 2 cut(s) 215, 291
BshFI GGCC 3 cut(s) 48, 510, 932
BshNI GGYRCC 2 cut(s) 503, 802
BshVI ATCGAT 1 cut(s) 297
BsiSI CCGG 3 cut(s) 205, 507, 807
BslI CCNNNNNNNGG 1 cut(s) 1045
BsmAI GTCTC 3 cut(s) 76, 356, 883
BsmBI CGTCTC 1 cut(s) 76
BsmI GAATGC 2 cut(s) 537, 951
BsnI GGCC 3 cut(s) 48, 510, 932
Bsp143I GATC 5 cut(s) 12, 207, 553, 647, 679
BspACI CCGC 5 cut(s) 36, 49, 243, 386, 565
BspANI GGCC 3 cut(s) 48, 510, 932
BspCNI CTCAG 4 cut(s) 102, 352, 708, 981
BspDI ATCGAT 1 cut(s) 297
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 505, 804
BspPI GGATC 3 cut(s) 215, 548, 674
BspT107I GGYRCC 2 cut(s) 503, 802
BsrDI GCAATG 1 cut(s) 1078
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 2 cut(s) 844, 1050
BssAI RCCGGY 1 cut(s) 506
BssMI GATC 5 cut(s) 12, 207, 553, 647, 679
Bst4CI ACNGT 3 cut(s) 134, 499, 1022
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 5 cut(s) 53, 240, 508, 963, 1069
BstDEI CTNAG 4 cut(s) 110, 360, 695, 989
BstF5I GGATG 5 cut(s) 404, 524, 708, 712, 769
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 5 cut(s) 15, 210, 556, 650, 682
BstMAI GTCTC 3 cut(s) 76, 356, 883
BstMBI GATC 5 cut(s) 12, 207, 553, 647, 679
BstMWI GCNNNNNNNGC 4 cut(s) 39, 48, 719, 850
BstSCI CCNGG 1 cut(s) 805
BstV1I GCAGC 3 cut(s) 20, 292, 1072
BstV2I GAAGAC 1 cut(s) 991
BstX2I RGATCY 2 cut(s) 553, 679
BstYI RGATCY 2 cut(s) 553, 679
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 3 cut(s) 48, 510, 932
BsuTUI ATCGAT 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 518
BtrI CACGTC 2 cut(s) 160, 476
BtsCI GGATG 5 cut(s) 404, 524, 708, 712, 769
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 4 cut(s) 371, 669, 703, 1043
Cac8I GCNNGC 5 cut(s) 53, 240, 508, 963, 1069
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 506
ClaI ATCGAT 1 cut(s) 297
CviAII CATG 2 cut(s) 412, 488
DdeI CTNAG 4 cut(s) 110, 360, 695, 989
DpnI GATC 5 cut(s) 14, 209, 555, 649, 681
DpnII GATC 5 cut(s) 12, 207, 553, 647, 679
DraIII CACNNNGTG 1 cut(s) 809
EaeI YGGCCR 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 932
Eco32I GATATC 2 cut(s) 865, 1105
Eco72I CACGTG 1 cut(s) 686
EcoRV GATATC 2 cut(s) 865, 1105
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 415, 491
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FatI CATG 2 cut(s) 411, 487
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 6 cut(s) 34, 49, 243, 306, 565, 1086
FokI GGATG 5 cut(s) 411, 511, 715, 719, 776
Fsp4HI GCNGC 6 cut(s) 34, 49, 243, 306, 565, 1086
FspBI CTAG 1 cut(s) 735
GlaI GCGC 1 cut(s) 153
GluI GCNGC 6 cut(s) 34, 49, 243, 306, 565, 1086
GsuI CTGGAG 4 cut(s) 570, 1031, 1083, 1110
HaeIII GGCC 3 cut(s) 48, 510, 932
HapII CCGG 3 cut(s) 205, 507, 807
HhaI GCGC 1 cut(s) 154
Hin1II CATG 2 cut(s) 415, 491
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 2 cut(s) 165, 495
HindII GTYRAC 2 cut(s) 165, 495
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 7 cut(s) 102, 272, 278, 491, 876, 971, 1092
HpaII CCGG 3 cut(s) 205, 507, 807
HphI GGTGA 3 cut(s) 22, 259, 370
Hpy166II GTNNAC 2 cut(s) 165, 495
Hpy188I TCNGA 5 cut(s) 27, 130, 217, 277, 361
Hpy188III TCNNGA 4 cut(s) 488, 549, 956, 1118
Hpy8I GTNNAC 2 cut(s) 165, 495
HpyAV CCTTC 3 cut(s) 95, 151, 521
HpyCH4III ACNGT 3 cut(s) 134, 499, 1022
HpyCH4IV ACGT 5 cut(s) 159, 475, 484, 685, 910
HpyCH4V TGCA 7 cut(s) 196, 232, 308, 405, 532, 713, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 48, 719, 850
HpyF3I CTNAG 4 cut(s) 110, 360, 695, 989
HpySE526I ACGT 5 cut(s) 159, 475, 484, 685, 910
Hsp92II CATG 2 cut(s) 415, 491
HspAI GCGC 1 cut(s) 152
KroI GCCGGC 1 cut(s) 506
KroNI GCCGGC 1 cut(s) 508
Kzo9I GATC 5 cut(s) 12, 207, 553, 647, 679
LmnI GCTCC 3 cut(s) 60, 1058, 1064
Lsp1109I GCAGC 3 cut(s) 20, 292, 1072
LweI GCATC 1 cut(s) 541
MaeI CTAG 1 cut(s) 735
MaeII ACGT 5 cut(s) 159, 475, 484, 685, 910
MaeIII GTNAC 3 cut(s) 476, 665, 998
MalI GATC 5 cut(s) 14, 209, 555, 649, 681
MboI GATC 5 cut(s) 12, 207, 553, 647, 679
MboII GAAGA 5 cut(s) 16, 216, 629, 836, 996
MflI RGATCY 2 cut(s) 553, 679
MluCI AATT 6 cut(s) 21, 184, 462, 630, 757, 827
MlyI GAGTC 3 cut(s) 500, 885, 1101
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 506
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 2 cut(s) 756, 771
MslI CAYNNNNRTG 1 cut(s) 410
MspI CCGG 3 cut(s) 205, 507, 807
MspR9I CCNGG 1 cut(s) 807
Mva1269I GAATGC 2 cut(s) 537, 951
MwoI GCNNNNNNNGC 4 cut(s) 39, 48, 719, 850
NaeI GCCGGC 1 cut(s) 508
NciI CCSGG 1 cut(s) 807
NdeII GATC 5 cut(s) 12, 207, 553, 647, 679
NgoMIV GCCGGC 1 cut(s) 506
NlaIII CATG 2 cut(s) 415, 491
NlaIV GGNNCC 2 cut(s) 505, 804
NmuCI GTSAC 2 cut(s) 476, 998
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 1 cut(s) 932
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PctI GAATGC 2 cut(s) 537, 951
PdiI GCCGGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 4 cut(s) 102, 272, 278, 971
PflMI CCANNNNNTGG 1 cut(s) 1045
PkrI GCNGC 6 cut(s) 35, 50, 244, 307, 566, 1087
PleI GAGTC 3 cut(s) 499, 884, 1100
PmaCI CACGTG 1 cut(s) 686
PmlI CACGTG 1 cut(s) 686
PpsI GAGTC 3 cut(s) 499, 884, 1100
Ppu21I YACGTR 1 cut(s) 686
PshBI ATTAAT 1 cut(s) 756
PsiI TTATAA 1 cut(s) 1032
PspCI CACGTG 1 cut(s) 686
PspN4I GGNNCC 2 cut(s) 505, 804
PsuI RGATCY 2 cut(s) 553, 679
RseI CAYNNNNRTG 1 cut(s) 410
SalI GTCGAC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 756, 771
SatI GCNGC 6 cut(s) 34, 49, 243, 306, 565, 1086
Sau3AI GATC 5 cut(s) 12, 207, 553, 647, 679
SchI GAGTC 3 cut(s) 500, 885, 1101
ScrFI CCNGG 1 cut(s) 807
SfaNI GCATC 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
Sse9I AATT 6 cut(s) 21, 184, 462, 630, 757, 827
SseBI AGGCCT 1 cut(s) 932
SsiI CCGC 5 cut(s) 36, 49, 243, 386, 565
SspMI CTAG 1 cut(s) 735
StuI AGGCCT 1 cut(s) 932
StyD4I CCNGG 1 cut(s) 805
TaaI ACNGT 3 cut(s) 134, 499, 1022
TaiI ACGT 5 cut(s) 162, 478, 487, 688, 913
TaqI TCGA 3 cut(s) 164, 297, 615
TasI AATT 6 cut(s) 21, 184, 462, 630, 757, 827
TauI GCSGC 3 cut(s) 51, 245, 567
TfiI GAWTC 4 cut(s) 102, 272, 278, 971
Tru1I TTAA 2 cut(s) 756, 771
Tru9I TTAA 2 cut(s) 756, 771
TscAI CASTG 4 cut(s) 378, 676, 703, 1050
TseFI GTSAC 2 cut(s) 476, 998
TseI GCWGC 3 cut(s) 33, 305, 1085
Tsp45I GTSAC 2 cut(s) 476, 998
TspDTI ATGAA 2 cut(s) 17, 534
TspRI CASTG 4 cut(s) 378, 676, 703, 1050
Van91I CCANNNNNTGG 1 cut(s) 1045
VspI ATTAAT 1 cut(s) 756
XapI RAATTY 1 cut(s) 827
XmiI GTMKAC 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.