pycom11g15480

homoserine dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008338
Physical Location & Seq
Forward (+)
16160704 .. 16165306
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g15480.10

Sequence Viewer

Length: 1134 bp
ATGAGCAAGAACATCTCTCTGATTCTGATGGGTTGCGGCGGCGTTGGGCGTCAGCTGCTCCAGCACATTGTCTCCTGCCGATCACTTCACGCCAAACAGGGAATCTACCTGAGAGTAGTGGGAGTAAGCGACAGTAAATCCTTACTCGTTGCATCCGACGCTTTCACCATGGAGCTGCCTGATGACTTTTTATTGGAAGTTTGCCGGGCCAAGTCGAATGGCGCTTCTCTCTCCACGCTCAGTGGTTATGGTGAGTTCCAGGTATTCGCGAATTCGGAATCAACCAAAAAGATTGTAGATGTTGCTGCATTCTTGGGTAAATCAACAGGTTTGGCACTTGTCGATTGCTCTGCCAGTTCTGATACTATTCCTTTACTAACCCAAGCGGTGGATTTGGATTGTTGCATTGTCATGGCAAATAAGAAGCCTCTTACTTCAACAATGGAGGACTATGACAAATTAGTTTCTCACCCACGTCACATTCGTCATGAGTCAACTGTTGGTGCTGGCCTCCCTGTCATAGCATCCTTAAATCGCATACTGTCATCTGGAGATCCTGTCCACCGCATTATCGGGAGTTTGAGTGGGACATTGGGGTATGTAATGAGTGAGCTTGAAGGTGGAAAGCTACTAAGTCAAGTTGTTCGTTCTGCTAAAAACCTGGGTTACACTGAACCAGATCCACGCGATGACCTTAGTGGGATGGATGTTGCAAGAAAGGCTTTGATACTAGCTAGGCTTCTTGGTCAGCGCATTAACTTGGATGACATTAAGATTGAGAGTTTGTATCCTGAAGAAATGGCACCTAGTGTAATGTCTATTGAAGACTTTCTGGGAAGTGGGCTATTATTGCTCGATAAAAATATCCAAGAGAGAGTCTCTAAGGCTTCTTTGGATGGGAAGGTGCTACGTTACGTCTGTGTGATTGAGGACTCAAGTTGTGAAGTTGGCATTCAAGAGCTTCCCAAGGATTCTGCTTTGGGAAGGCTGAGAGGAAGTGACAATGTGTTGGAGGTTTACAGTCGATGCTATAAAGACCAACCGCTGGTGATTCAAGGAGCTGGAGCCGGCAATGATACTACAGCAGCTGGTGTCCTTGCTGATATCGTTGATATTCAGGATTTATTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homoserine_dh NAD_binding_3

Protein Analysis

378

Amino Acids

40.4

Weight (kDa)

5.18

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 802
AccB7I CCANNNNNTGG 2 cut(s) 388, 1045
AccII CGCG 2 cut(s) 269, 687
AciI CCGC 5 cut(s) 36, 39, 386, 565, 1043
AclWI GGATC 2 cut(s) 548, 674
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 813
AcyI GRCGYC 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 809
AfiI CCNNNNNNNGG 2 cut(s) 388, 1045
AgsI TTSAA 5 cut(s) 438, 617, 824, 956, 1055
AjiI CACGTC 1 cut(s) 476
AjnI CCWGG 2 cut(s) 258, 660
AluBI AGCT 8 cut(s) 55, 175, 613, 628, 734, 961, 1061, 1088
AluI AGCT 8 cut(s) 55, 175, 613, 628, 734, 961, 1061, 1088
Alw26I GTCTC 2 cut(s) 76, 883
AlwI GGATC 2 cut(s) 548, 674
AlwNI CAGNNNCTG 1 cut(s) 1088
AoxI GGCC 2 cut(s) 207, 508
ApeKI GCWGC 4 cut(s) 55, 175, 305, 1085
ApoI RAATTY 1 cut(s) 271
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 2 cut(s) 224, 753
AspS9I GGNCC 1 cut(s) 207
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 4 cut(s) 157, 263, 461, 1060
BanI GGYRCC 1 cut(s) 802
BbsI GAAGAC 1 cut(s) 831
BbvI GCAGC 4 cut(s) 42, 162, 292, 1097
BccI CCATC 3 cut(s) 22, 697, 890
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 260, 662
BciVI GTATCC 1 cut(s) 798
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 76, 883
BfaI CTAG 3 cut(s) 731, 735, 807
BfmI CTRYAG 1 cut(s) 1080
BfoI RGCGCY 1 cut(s) 225
BfuI GTATCC 1 cut(s) 798
BisI GCNGC 6 cut(s) 37, 40, 56, 176, 306, 1086
BlsI GCNGC 6 cut(s) 38, 41, 57, 177, 307, 1087
Bme1390I CCNGG 3 cut(s) 206, 260, 662
BmgBI CACGTC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 804, 1066
BmrFI CCNGG 3 cut(s) 206, 260, 662
BmsI GCATC 3 cut(s) 161, 533, 1016
BpiI GAAGAC 1 cut(s) 831
BplI GAGNNNNNCTC 2 cut(s) 863, 895
BpmI CTGGAG 3 cut(s) 44, 570, 1083
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 168, 661, 966
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 543, 568, 573, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 1045
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 1078
BseBI CCWGG 2 cut(s) 260, 662
BseDI CCNNGG 3 cut(s) 168, 661, 966
BseGI GGATG 6 cut(s) 152, 524, 708, 712, 769, 901
BseLI CCNNNNNNNGG 2 cut(s) 388, 1045
BseMI GCAATG 1 cut(s) 1078
BseMII CTCAG 3 cut(s) 101, 253, 980
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 4 cut(s) 42, 162, 292, 1097
Bsh1236I CGCG 2 cut(s) 269, 687
BshFI GGCC 2 cut(s) 209, 510
BshNI GGYRCC 1 cut(s) 802
BsiSI CCGG 2 cut(s) 205, 1068
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 2 cut(s) 388, 1045
BsmAI GTCTC 2 cut(s) 76, 883
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 2 cut(s) 308, 951
BsnI GGCC 2 cut(s) 209, 510
Bsp143I GATC 3 cut(s) 80, 553, 679
Bsp19I CCATGG 1 cut(s) 168
Bsp68I TCGCGA 1 cut(s) 269
BspACI CCGC 5 cut(s) 36, 39, 386, 565, 1043
BspANI GGCC 2 cut(s) 209, 510
BspCNI CTCAG 3 cut(s) 102, 252, 981
BspFNI CGCG 2 cut(s) 269, 687
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 804, 1066
BspPI GGATC 2 cut(s) 548, 674
BspT107I GGYRCC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 1078
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 3 cut(s) 168, 661, 966
BssMI GATC 3 cut(s) 80, 553, 679
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 168, 966
Bst2UI CCWGG 2 cut(s) 260, 662
Bst4CI ACNGT 4 cut(s) 134, 499, 543, 1022
BstACI GRCGYC 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 508, 1069
BstDEI CTNAG 6 cut(s) 110, 239, 632, 695, 882, 989
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 6 cut(s) 152, 524, 708, 712, 769, 901
BstFNI CGCG 2 cut(s) 269, 687
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 224, 753
BstKTI GATC 3 cut(s) 83, 556, 682
BstMAI GTCTC 2 cut(s) 76, 883
BstMBI GATC 3 cut(s) 80, 553, 679
BstMWI GCNNNNNNNGC 5 cut(s) 55, 61, 158, 719, 850
BstNI CCWGG 2 cut(s) 260, 662
BstSCI CCNGG 3 cut(s) 204, 258, 660
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 2 cut(s) 269, 687
BstV1I GCAGC 4 cut(s) 42, 162, 292, 1097
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 2 cut(s) 553, 679
BstYI RGATCY 2 cut(s) 553, 679
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 2 cut(s) 209, 510
BtgI CCRYGG 1 cut(s) 168
BtgZI GCGATG 1 cut(s) 702
BtrI CACGTC 1 cut(s) 476
BtsCI GGATG 6 cut(s) 152, 524, 708, 712, 769, 901
BtsIMutI CAGTG 2 cut(s) 247, 669
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 508, 1069
CaiI CAGNNNCTG 1 cut(s) 1088
CciI TCATGA 1 cut(s) 487
CfoI GCGC 2 cut(s) 224, 753
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 2 cut(s) 38, 167
CviAII CATG 3 cut(s) 169, 412, 488
DdeI CTNAG 6 cut(s) 110, 239, 632, 695, 882, 989
DpnI GATC 3 cut(s) 82, 555, 681
DpnII GATC 3 cut(s) 80, 553, 679
DraIII CACNNNGTG 1 cut(s) 809
Eco130I CCWWGG 2 cut(s) 168, 966
Eco32I GATATC 1 cut(s) 1105
Eco57I CTGAAG 1 cut(s) 813
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 258, 660
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 2 cut(s) 168, 966
ErhI CCWWGG 2 cut(s) 168, 966
FaeI CATG 3 cut(s) 172, 415, 491
FaiI YATR 9 cut(s) 170, 249, 413, 453, 489, 521, 539, 600, 1032
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FaqI GGGAC 1 cut(s) 601
FatI CATG 3 cut(s) 168, 411, 487
Fnu4HI GCNGC 6 cut(s) 37, 40, 56, 176, 306, 1086
FokI GGATG 6 cut(s) 139, 511, 715, 719, 776, 908
Fsp4HI GCNGC 6 cut(s) 37, 40, 56, 176, 306, 1086
FspBI CTAG 3 cut(s) 731, 735, 807
GlaI GCGC 2 cut(s) 223, 752
GluI GCNGC 6 cut(s) 37, 40, 56, 176, 306, 1086
GsuI CTGGAG 3 cut(s) 44, 570, 1083
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 2 cut(s) 209, 510
HapII CCGG 2 cut(s) 205, 1068
HgaI GACGC 2 cut(s) 38, 167
HhaI GCGC 2 cut(s) 224, 753
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 3 cut(s) 172, 415, 491
Hin6I GCGC 2 cut(s) 222, 751
HinP1I GCGC 2 cut(s) 222, 751
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HinfI GANTC 8 cut(s) 22, 102, 278, 491, 876, 932, 971, 1051
HpaII CCGG 2 cut(s) 205, 1068
HphI GGTGA 4 cut(s) 157, 263, 461, 1060
Hpy166II GTNNAC 3 cut(s) 495, 562, 1018
Hpy188I TCNGA 5 cut(s) 21, 27, 157, 277, 361
Hpy188III TCNNGA 7 cut(s) 268, 488, 549, 574, 791, 956, 1118
Hpy8I GTNNAC 3 cut(s) 495, 562, 1018
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 611, 895, 978
HpyCH4III ACNGT 4 cut(s) 134, 499, 543, 1022
HpyCH4IV ACGT 3 cut(s) 475, 910, 915
HpyCH4V TGCA 4 cut(s) 152, 308, 405, 713
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 61, 158, 719, 850
HpyF3I CTNAG 6 cut(s) 110, 239, 632, 695, 882, 989
HpySE526I ACGT 3 cut(s) 475, 910, 915
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 3 cut(s) 172, 415, 491
HspAI GCGC 2 cut(s) 222, 751
KroI GCCGGC 1 cut(s) 1067
KroNI GCCGGC 1 cut(s) 1069
Kzo9I GATC 3 cut(s) 80, 553, 679
LmnI GCTCC 4 cut(s) 63, 172, 1058, 1064
Lsp1109I GCAGC 4 cut(s) 42, 162, 292, 1097
LweI GCATC 3 cut(s) 161, 533, 1016
MaeI CTAG 3 cut(s) 731, 735, 807
MaeII ACGT 3 cut(s) 475, 910, 915
MaeIII GTNAC 4 cut(s) 476, 665, 911, 998
MalI GATC 3 cut(s) 82, 555, 681
MboI GATC 3 cut(s) 80, 553, 679
MboII GAAGA 2 cut(s) 806, 836
MflI RGATCY 2 cut(s) 553, 679
MluCI AATT 2 cut(s) 271, 458
MlyI GAGTC 3 cut(s) 500, 885, 926
MmeI TCCRAC 2 cut(s) 180, 990
MnlI CCTC 6 cut(s) 438, 439, 521, 922, 986, 1006
MroNI GCCGGC 1 cut(s) 1067
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 3 cut(s) 530, 756, 771
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 3 cut(s) 55, 1045, 1088
MspI CCGG 2 cut(s) 205, 1068
MspR9I CCNGG 3 cut(s) 206, 260, 662
Mva1269I GAATGC 2 cut(s) 308, 951
MvaI CCWGG 2 cut(s) 260, 662
MvnI CGCG 2 cut(s) 269, 687
MwoI GCNNNNNNNGC 5 cut(s) 55, 61, 158, 719, 850
NaeI GCCGGC 1 cut(s) 1069
NciI CCSGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 80, 553, 679
NgoMIV GCCGGC 1 cut(s) 1067
NlaIII CATG 3 cut(s) 172, 415, 491
NlaIV GGNNCC 2 cut(s) 804, 1066
NmuCI GTSAC 2 cut(s) 476, 998
NruI TCGCGA 1 cut(s) 269
PagI TCATGA 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 481
PctI GAATGC 2 cut(s) 308, 951
PdiI GCCGGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 5 cut(s) 22, 102, 278, 971, 1051
PflMI CCANNNNNTGG 2 cut(s) 388, 1045
PkrI GCNGC 6 cut(s) 38, 41, 57, 177, 307, 1087
PleI GAGTC 3 cut(s) 499, 884, 926
PpsI GAGTC 3 cut(s) 499, 884, 926
Psp6I CCWGG 2 cut(s) 258, 660
PspGI CCWGG 2 cut(s) 258, 660
PspN4I GGNNCC 2 cut(s) 804, 1066
PspPI GGNCC 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 1088
PsuI RGATCY 2 cut(s) 553, 679
PvuII CAGCTG 2 cut(s) 55, 1088
RruI TCGCGA 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 3 cut(s) 530, 756, 771
SatI GCNGC 6 cut(s) 37, 40, 56, 176, 306, 1086
Sau3AI GATC 3 cut(s) 80, 553, 679
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 3 cut(s) 500, 885, 926
ScrFI CCNGG 3 cut(s) 206, 260, 662
SfaNI GCATC 3 cut(s) 161, 533, 1016
SfcI CTRYAG 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
Sse9I AATT 2 cut(s) 271, 458
SsiI CCGC 5 cut(s) 36, 39, 386, 565, 1043
SspMI CTAG 3 cut(s) 731, 735, 807
StyD4I CCNGG 3 cut(s) 204, 258, 660
StyI CCWWGG 2 cut(s) 168, 966
TaaI ACNGT 4 cut(s) 134, 499, 543, 1022
TaiI ACGT 3 cut(s) 478, 913, 918
TaqI TCGA 4 cut(s) 215, 342, 855, 1024
TasI AATT 2 cut(s) 271, 458
TauI GCSGC 2 cut(s) 39, 42
TfiI GAWTC 5 cut(s) 22, 102, 278, 971, 1051
Tru1I TTAA 3 cut(s) 530, 756, 771
Tru9I TTAA 3 cut(s) 530, 756, 771
TscAI CASTG 2 cut(s) 247, 676
TseFI GTSAC 2 cut(s) 476, 998
TseI GCWGC 4 cut(s) 55, 175, 305, 1085
Tsp45I GTSAC 2 cut(s) 476, 998
TspRI CASTG 2 cut(s) 247, 676
Van91I CCANNNNNTGG 2 cut(s) 388, 1045
XapI RAATTY 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 3 cut(s) 731, 735, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.