AT5G22770

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007314
Physical Location & Seq
Reverse (-)
7579156 .. 7588322
9167 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22770.1

Sequence Viewer

Length: 3039 bp
ATGACCGGAATGAGAGGTCTCTCCGTATTTATCAGCGACGTTCGTAATTGCCAGAACAAGGAAGCGGAGAGACTACGAGTTGATAAGGAGCTCGGAAACATTCGTACATGCTTCAAGAATGAGAAGGTTTTGACACCATATAAAAAGAAGAAATATGTCTGGAAAATGCTTTATATACATATGCTAGGCTACGATGTGGATTTTGGCCATATGGAGGCGGTTTCTCTTATATCTGCGCCAAAGTATCCAGAAAAGCAGGTTGGCTACATTGTTACATCTTGCTTGCTCAATGAGAACCATGACTTTTTGAAGTTGGCAATAAATACAGTGCGGAACGATATTATTGGTCGGAATGAGACTTTCCAGTGCCTAGCACTGACCTTGGTTGGAAATATCGGTGGAAGGGATTTTGCTGAATCATTGGCACCTGATGTTCAAAAGCTACTTATTTCTAGTAGTTGCAGACCTCTTGTAAGGAAAAAAGCAGCACTGTGCCTACTGCGCTTATTCCGAAAAAATCCTGATGCTGTCAACGTGGATGGCTGGGCGGATCGTATGGCACAACTTTTGGATGAACGAGATCTTGGTGTCTTGACGTCTTCTACGAGTCTTCTTGTAGCTCTGGTATCTAATAATCATGAGGCGTACTCAAGTTGTCTACCTAAATGTGTCAAAATATTAGAACGCCTTGCTAGGAACCAAGATGTTCCTCAGGAGTACACATACTATGGCATCCCATCTCCTTGGCTCCAGGTTAAGGCAATGAGGGCTCTTCAATATTTCCCGACCATTGAAGATCCCAGTACCAGAAAAGCTCTGTTTGAGGTCCTTCAGCGAATATTGATGGGGACAGATGTTGTGAAAAATGTGAATAAAAACAATGCGTCTCATGCTGTGCTGTTTGAAGCTCTATCCCTGGTCATGCATCTTGATGCTGAGAAGGAAATGATGTCACAGTGTGTTGCTCTTCTTGGCAAGTTTATTTCTGTCCGTGAGCCCAACATCAGATATCTAGGCTTGGAAAATATGACGCGGATGCTGATGGTCACAGATGTTCAAGATATCATAAAGAAGCATCAGTCTCAGATAATCACCTCCTTGAAAGACCCTGACATCAGTATTCGTAGGCGTGCTCTTGATTTATTATATGGGATGTGTGATGTGTCAAATGCAAAGGATATAGTCGAAGAGTTGCTGCAGTATCTTAGCACAGCAGAGTTTTCCATGCGGGAAGAATTATCACTTAAAGCTGCTATCCTTGCTGAGAAGTTTGCTCCTGATCTATCATGGTATGTTGATGTGATCCTTCAGTTGATCGACAAGGCTGGGGATTTTGTTAGCGATGACATATGGTTTCGTGTGGTTCAGTTTGTAACAAATAATGAAGACCTGCAGCCTTACGCCGCGTCAAAAGCAAGAGAATATTTGGACAAAATCGCAATACATGAGACTATGGTGAAGGTCAGTGCTTATATTTTGGGGGAGTATGGTCACCTTCTAGCTAGACAACCTGGATGCAGTGCAAGTGAACTCTTTAGCATCTTACATGAGAAGCTTCCTACAATATCGACTCCCACCATTCCTATTCTTCTTTCCACATATGCAAAGCTTCTTATGCACGCTCAACCCCCGGATCCAGAACTGCAAAAAAAAGTTTGGGCTGTATTTAAGAAATATGAGAGTTGTATCGATGTGGAGATACAACAGAGGGCAGTTGAATATTTTGAATTGAGTAAAAAAGGGCCTGCCTTTATGGATGTATTGGCTGAGATGCCAAAATTTCCGGAGCGCCAGTCTTCATTGATTAAAAAGGCAGAAAACGTTGAAGATACCGCAGACCAGAGTGCCATCAAACTTAGAGCACAACAACAGCCTTCAAATGCTATGGTTTTGGCTGACCAACAGCCTGTTAATGGGGCACCACCACCTTTGAAGGTTCCAATTTTAAGTGGAAGCACGGACCCTGAGTCAGTGGCTCGATCTCTCTCACATCCCAATGGAACTTTAAGTAATATAGATCCACAAACTCCATCACCTGATCTCCTTAGTGACCTACTCGGTCCTCTTGCAATAGAGGCTCCACCTGGAGCTGTCTCAAATGAACAACATGGTCCCGTAGGGGCAGAAGGAGTTCCAGATGAAGTTGATGGTTCCGCCATTGTACCTGTTGAAGAGCAAACCAATACAGTGGAGCTTATCGGAAATATTGCTGAGAGATTTCATGCTTTATGTTTGAAAGACAGTGGTGTCTTATACGAGGATCCTCATATCCAGATTGGCATCAAAGCTGAATGGAGAGGACACCATGGTAGACTTGTTCTCTTCATGGGAAACAAGAATACTTCTCCACTTACATCAGTTCAAGCTCTGATACTACCCCCTGCCCACTTAAGACTGGATCTGTCTCCAGTACCTGATACAATTCCTCCTCGAGCACAGGTACAATCTCCACTTGAAGTGATGAACATCCGCCCTAGCAGGGATGTTGCGGTTTTGGATTTCTCTTACAAGTTTGGTGCAAACGTGGTCAGTGCCAAATTACGGATCCCAGCAACCTTAAATAAGTTTCTTCAACCTCTACAATTGACATCTGAAGAGTTCTTCCCGCAATGGAGAGCAATCTCAGGTCCACCCTTGAAACTTCAGGAAGTGGTTAGAGGTGTAAGACCTCTAGCTCTTCCGGAAATGGCAAATCTGTTCAACAGTTTTCATGTGACGATATGCCCTGGGCTTGATCCAAACCCTAATAATCTAGTGGCAAGTACAACCTTCTACTCTGAAAGTACAGGAGCCATTCTCTGTTTGGCAAGAATAGAAACAGACCCAGCGGACAGAACCCAATTGCGAATGACAGTAGGCACGGGAGATCCTACCCTTACATTTGAGTTGAAAGAATTTATCAAGGAACAACTAATTACTGTCCCAATGGGATCTCGAGCTTTAGTTCCAGCAGCAGGTCCAGCACCACCTGTAGCTCAACCGCCAAGCCCAGCGGCTTTAGCTGATGATCCAGGAGCTATGCTCGCTGGCTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1012

Amino Acids

112.15

Weight (kDa)

5.85

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 580 5.7e-117 Adaptin N terminal region
Cnd1 PF12717 123 - 261 3.1e-07 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 748 - 842 2.2e-16 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 856 - 965 2.1e-16 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2246
AatII GACGTC 1 cut(s) 599
Acc36I ACCTGC 3 cut(s) 247, 1398, 2945
AccB1I GGYRCC 2 cut(s) 424, 1918
AccI GTMKAC 2 cut(s) 658, 2311
AccII CGCG 2 cut(s) 1033, 1406
AccIII TCCGGA 2 cut(s) 1783, 2680
AclI AACGTT 1 cut(s) 1821
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 1778, 2894
AcuI CTGAAG 4 cut(s) 815, 1292, 2613, 2627
AcyI GRCGYC 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 959
AfaI GTAC 9 cut(s) 106, 647, 719, 805, 2163, 2412, 2442, 2764, 2785
AfiI CCNNNNNNNGG 7 cut(s) 58, 214, 757, 1913, 2479, 2541, 2954
AflII CTTAAG 1 cut(s) 2389
AhdI GACNNNNNGTC 1 cut(s) 1966
AjnI CCWGG 6 cut(s) 750, 915, 1510, 2083, 2725, 3010
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 567, 599
Alw21I GWGCWC 4 cut(s) 93, 1135, 1864, 2437
Alw26I GTCTC 8 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2409
AlwNI CAGNNNCTG 1 cut(s) 2414
Ama87I CYCGRG 2 cut(s) 2430, 2934
Aor13HI TCCGGA 2 cut(s) 1783, 2680
AoxI GGCC 2 cut(s) 205, 1742
ApeKI GCWGC 5 cut(s) 485, 1195, 1250, 1393, 2951
ApoI RAATTY 2 cut(s) 1778, 2894
Asp700I GAANNNNTTC 1 cut(s) 2130
AspLEI GCGC 3 cut(s) 238, 504, 1791
AspS9I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
AsuC2I CCSGG 1 cut(s) 1631
AsuHPI GGTGA 4 cut(s) 1084, 1468, 1484, 2025
AvaI CYCGRG 2 cut(s) 2430, 2934
AvaII GGWCC 6 cut(s) 826, 1960, 2060, 2111, 2627, 2957
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 1 cut(s) 1921
BalI TGGCCA 1 cut(s) 207
BamHI GGATCC 3 cut(s) 1633, 2260, 2544
BanI GGYRCC 2 cut(s) 424, 1918
BanII GRGCYC 3 cut(s) 93, 772, 999
BbsI GAAGAC 4 cut(s) 591, 602, 1392, 1788
Bbv12I GWGCWC 4 cut(s) 93, 1135, 1864, 2437
BbvI GCAGC 5 cut(s) 497, 1182, 1237, 1405, 2963
BccI CCATC 7 cut(s) 533, 745, 838, 1036, 1856, 2038, 2141
BciT130I CCWGG 6 cut(s) 752, 917, 1512, 2085, 2727, 3012
BciVI GTATCC 1 cut(s) 255
BcnI CCSGG 1 cut(s) 1631
BcoDI GTCTC 8 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2409
BfmI CTRYAG 3 cut(s) 1196, 1391, 2970
BfoI RGCGCY 1 cut(s) 1792
BfrI CTTAAG 1 cut(s) 2389
BfuAI ACCTGC 3 cut(s) 247, 1398, 2945
BfuI GTATCC 1 cut(s) 255
BglII AGATCT 1 cut(s) 580
BisI GCNGC 7 cut(s) 486, 1196, 1251, 1394, 1404, 2952, 2994
BlsI GCNGC 7 cut(s) 487, 1197, 1252, 1395, 1405, 2953, 2995
Bme1390I CCNGG 7 cut(s) 752, 917, 1512, 1631, 2085, 2727, 3012
Bme18I GGWCC 6 cut(s) 826, 1960, 2060, 2111, 2627, 2957
BmeRI GACNNNNNGTC 1 cut(s) 1966
BmeT110I CYCGRG 2 cut(s) 2430, 2934
BmgT120I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
BmrFI CCNGG 7 cut(s) 752, 917, 1512, 1631, 2085, 2727, 3012
BmrI ACTGGG 1 cut(s) 795
BmuI ACTGGG 1 cut(s) 795
BpiI GAAGAC 4 cut(s) 591, 602, 1392, 1788
BplI GAGNNNNNCTC 6 cut(s) 632, 664, 2781, 2813, 3006, 3038
BpmI CTGGAG 3 cut(s) 734, 2106, 2391
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 1631
Bsa29I ATCGAT 1 cut(s) 1689
BsaBI GATNNNNATC 2 cut(s) 2279, 2465
BsaHI GRCGYC 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 7 cut(s) 381, 743, 915, 1629, 2305, 2725, 2726
BsaWI WCCGGW 3 cut(s) 5, 1783, 2680
BsaXI ACNNNNNCTCC 6 cut(s) 1778, 1808, 2025, 2055, 2410, 2440
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 214, 757, 1913, 2479, 2541, 2954
Bse1I ACTGG 5 cut(s) 364, 801, 1792, 2400, 2408
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 2 cut(s) 768, 2615
Bse8I GATNNNNATC 2 cut(s) 2279, 2465
BseAI TCCGGA 2 cut(s) 1783, 2680
BseBI CCWGG 6 cut(s) 752, 917, 1512, 2085, 2727, 3012
BseCI ATCGAT 1 cut(s) 1689
BseDI CCNNGG 7 cut(s) 381, 743, 915, 1629, 2305, 2725, 2726
BseJI GATNNNNATC 2 cut(s) 2279, 2465
BseLI CCNNNNNNNGG 7 cut(s) 58, 214, 757, 1913, 2479, 2541, 2954
BseMI GCAATG 2 cut(s) 768, 2615
BseMII CTCAG 8 cut(s) 725, 927, 1097, 1254, 1758, 1956, 2202, 2637
BseNI ACTGG 5 cut(s) 364, 801, 1792, 2400, 2408
BseRI GAGGAG 1 cut(s) 2418
BseSI GKGCMC 1 cut(s) 1921
BseXI GCAGC 5 cut(s) 497, 1182, 1237, 1405, 2963
BseYI CCCAGC 5 cut(s) 543, 1325, 2548, 2824, 2989
Bsh1236I CGCG 2 cut(s) 1033, 1406
BshFI GGCC 2 cut(s) 207, 1744
BshNI GGYRCC 2 cut(s) 424, 1918
BshVI ATCGAT 1 cut(s) 1689
BsiHKAI GWGCWC 4 cut(s) 93, 1135, 1864, 2437
BsiHKCI CYCGRG 2 cut(s) 2430, 2934
BsiSI CCGG 4 cut(s) 6, 1631, 1784, 2681
BslFI GGGAC 3 cut(s) 862, 2097, 2906
BslI CCNNNNNNNGG 7 cut(s) 58, 214, 757, 1913, 2479, 2541, 2954
BsmAI GTCTC 8 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2409
BsmBI CGTCTC 1 cut(s) 891
BsmFI GGGAC 3 cut(s) 862, 2097, 2906
BsnI GGCC 2 cut(s) 207, 1744
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 2 cut(s) 2430, 2934
Bsp1286I GDGCHC 7 cut(s) 93, 772, 999, 1135, 1864, 1921, 2437
Bsp13I TCCGGA 2 cut(s) 1783, 2680
Bsp19I CCATGG 1 cut(s) 2305
BspANI GGCC 2 cut(s) 207, 1744
BspCNI CTCAG 8 cut(s) 724, 928, 1096, 1255, 1759, 1957, 2203, 2636
BspDI ATCGAT 1 cut(s) 1689
BspEI TCCGGA 2 cut(s) 1783, 2680
BspFNI CGCG 2 cut(s) 1033, 1406
BspHI TCATGA 1 cut(s) 637
BspMAI CTGCAG 2 cut(s) 1200, 1395
BspMI ACCTGC 3 cut(s) 247, 1398, 2945
BspQI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
BspT107I GGYRCC 2 cut(s) 424, 1918
BspTI CTTAAG 1 cut(s) 2389
BspTNI GGTCTC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 768, 2615
BsrI ACTGG 5 cut(s) 364, 801, 1792, 2400, 2408
BssECI CCNNGG 7 cut(s) 381, 743, 915, 1629, 2305, 2725, 2726
BssNI GRCGYC 1 cut(s) 596
BssT1I CCWWGG 3 cut(s) 381, 743, 2305
Bst2UI CCWGG 6 cut(s) 752, 917, 1512, 2085, 2727, 3012
Bst4CI ACNGT 8 cut(s) 328, 492, 957, 2188, 2243, 2705, 2854, 2920
Bst6I CTCTTC 7 cut(s) 777, 972, 1182, 2166, 2327, 2589, 2682
BstACI GRCGYC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 2389
BstC8I GCNNGC 6 cut(s) 284, 1131, 1620, 1746, 3024, 3028
BstDSI CCRYGG 1 cut(s) 2305
BstEII GGTNACC 1 cut(s) 1490
BstFNI CGCG 2 cut(s) 1033, 1406
BstH2I RGCGCY 1 cut(s) 1792
BstHHI GCGC 3 cut(s) 238, 504, 1791
BstMAI GTCTC 8 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2409
BstNI CCWGG 6 cut(s) 752, 917, 1512, 2085, 2727, 3012
BstNSI RCATGY 1 cut(s) 111
BstPI GGTNACC 1 cut(s) 1490
BstSCI CCNGG 7 cut(s) 750, 915, 1510, 1629, 2083, 2725, 3010
BstSFI CTRYAG 3 cut(s) 1196, 1391, 2970
BstSLI GKGCMC 1 cut(s) 1921
BstUI CGCG 2 cut(s) 1033, 1406
BstV1I GCAGC 5 cut(s) 497, 1182, 1237, 1405, 2963
BstV2I GAAGAC 4 cut(s) 591, 602, 1392, 1788
BstX2I RGATCY 9 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2544, 2866, 2930
BstXI CCANNNNNNTGG 2 cut(s) 744, 2188
BstYI RGATCY 9 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2544, 2866, 2930
Bsu15I ATCGAT 1 cut(s) 1689
Bsu36I CCTNAGG 1 cut(s) 711
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 2 cut(s) 207, 1744
BsuTUI ATCGAT 1 cut(s) 1689
BtgI CCRYGG 1 cut(s) 2305
BtgZI GCGATG 1 cut(s) 1356
BtsI GCAGTG 1 cut(s) 1525
BveI ACCTGC 3 cut(s) 247, 1398, 2945
Cac8I GCNNGC 6 cut(s) 284, 1131, 1620, 1746, 3024, 3028
CaiI CAGNNNCTG 1 cut(s) 2414
CciI TCATGA 1 cut(s) 637
CfoI GCGC 3 cut(s) 238, 504, 1791
Cfr13I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
ClaI ATCGAT 1 cut(s) 1689
CseI GACGC 3 cut(s) 873, 1039, 1395
Csp6I GTAC 9 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2763, 2784
CspCI CAANNNNNGTGG 2 cut(s) 1911, 1946
CviQI GTAC 9 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2763, 2784
DraIII CACNNNGTG 1 cut(s) 959
DrdI GACNNNNNNGTC 1 cut(s) 2246
DriI GACNNNNNGTC 1 cut(s) 1966
DseDI GACNNNNNNGTC 1 cut(s) 2246
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 7 cut(s) 777, 972, 1182, 2166, 2327, 2589, 2682
Eam1105I GACNNNNNGTC 1 cut(s) 1966
EarI CTCTTC 7 cut(s) 777, 972, 1182, 2166, 2327, 2589, 2682
EciI GGCGGA 3 cut(s) 563, 2143, 2459
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 3 cut(s) 381, 743, 2305
Eco24I GRGCYC 3 cut(s) 93, 772, 999
Eco31I GGTCTC 1 cut(s) 23
Eco32I GATATC 2 cut(s) 1010, 1063
Eco47I GGWCC 6 cut(s) 826, 1960, 2060, 2111, 2627, 2957
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 4 cut(s) 815, 1292, 2613, 2627
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 2 cut(s) 2430, 2934
Eco91I GGTNACC 1 cut(s) 1490
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 2 cut(s) 826, 1742
EcoO65I GGTNACC 1 cut(s) 1490
EcoRII CCWGG 6 cut(s) 750, 915, 1510, 2083, 2725, 3010
EcoRV GATATC 2 cut(s) 1010, 1063
EcoT14I CCWWGG 3 cut(s) 381, 743, 2305
EcoT22I ATGCAT 1 cut(s) 927
EcoT38I GRGCYC 3 cut(s) 93, 772, 999
ErhI CCWWGG 3 cut(s) 381, 743, 2305
Esp3I CGTCTC 1 cut(s) 891
FaqI GGGAC 3 cut(s) 862, 2097, 2906
FauI CCCGC 2 cut(s) 1221, 2613
FauNDI CATATG 4 cut(s) 180, 210, 1349, 1600
FblI GTMKAC 2 cut(s) 658, 2311
Fnu4HI GCNGC 7 cut(s) 486, 1196, 1251, 1394, 1404, 2952, 2994
FriOI GRGCYC 3 cut(s) 93, 772, 999
Fsp4HI GCNGC 7 cut(s) 486, 1196, 1251, 1394, 1404, 2952, 2994
GlaI GCGC 3 cut(s) 237, 503, 1790
GluI GCNGC 7 cut(s) 486, 1196, 1251, 1394, 1404, 2952, 2994
GsaI CCCAGC 5 cut(s) 547, 1329, 2552, 2828, 2993
GsuI CTGGAG 3 cut(s) 734, 2106, 2391
HaeII RGCGCY 1 cut(s) 1792
HaeIII GGCC 2 cut(s) 207, 1744
HapII CCGG 4 cut(s) 6, 1631, 1784, 2681
HgaI GACGC 3 cut(s) 873, 1039, 1395
HhaI GCGC 3 cut(s) 238, 504, 1791
Hin1I GRCGYC 1 cut(s) 596
Hin6I GCGC 3 cut(s) 236, 502, 1789
HinP1I GCGC 3 cut(s) 236, 502, 1789
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 2 cut(s) 1553, 1607
HinfI GANTC 4 cut(s) 416, 607, 1570, 1967
HpaII CCGG 4 cut(s) 6, 1631, 1784, 2681
HphI GGTGA 4 cut(s) 1084, 1468, 1484, 2025
Hpy166II GTNNAC 6 cut(s) 532, 659, 720, 1529, 2312, 2630
Hpy188I TCNGA 9 cut(s) 95, 351, 512, 1007, 1086, 2201, 2370, 2593, 2779
Hpy8I GTNNAC 6 cut(s) 532, 659, 720, 1529, 2312, 2630
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 8 cut(s) 328, 492, 957, 2188, 2243, 2705, 2854, 2920
HpyCH4IV ACGT 5 cut(s) 39, 534, 596, 1821, 2523
HpySE526I ACGT 5 cut(s) 39, 534, 596, 1821, 2523
Hsp92I GRCGYC 1 cut(s) 596
HspAI GCGC 3 cut(s) 236, 502, 1789
Kpn2I TCCGGA 2 cut(s) 1783, 2680
LguI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
LmnI GCTCC 9 cut(s) 88, 753, 1279, 1786, 2083, 2087, 2191, 2789, 3014
Lsp1109I GCAGC 5 cut(s) 497, 1182, 1237, 1405, 2963
MaeII ACGT 5 cut(s) 39, 534, 596, 1821, 2523
MaeIII GTNAC 7 cut(s) 271, 951, 1045, 1372, 1490, 2048, 2713
MfeI CAATTG 2 cut(s) 2582, 2840
MflI RGATCY 9 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2544, 2866, 2930
MhlI GDGCHC 7 cut(s) 93, 772, 999, 1135, 1864, 1921, 2437
MlsI TGGCCA 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 3 cut(s) 616, 1564, 1976
MmeI TCCRAC 2 cut(s) 329, 367
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 927
MroI TCCGGA 2 cut(s) 1783, 2680
MroXI GAANNNNTTC 1 cut(s) 2130
MscI TGGCCA 1 cut(s) 207
MslI CAYNNNNRTG 2 cut(s) 930, 1995
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 2828, 2993
MspCI CTTAAG 1 cut(s) 2389
MspI CCGG 4 cut(s) 6, 1631, 1784, 2681
MspR9I CCNGG 7 cut(s) 752, 917, 1512, 1631, 2085, 2727, 3012
MunI CAATTG 2 cut(s) 2582, 2840
MvaI CCWGG 6 cut(s) 752, 917, 1512, 2085, 2727, 3012
MvnI CGCG 2 cut(s) 1033, 1406
NciI CCSGG 1 cut(s) 1631
NcoI CCATGG 1 cut(s) 2305
NdeI CATATG 4 cut(s) 180, 210, 1349, 1600
NmuCI GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
NsiI ATGCAT 1 cut(s) 927
NspI RCATGY 1 cut(s) 111
PaeR7I CTCGAG 2 cut(s) 2430, 2934
PagI TCATGA 1 cut(s) 637
PasI CCCWGGG 1 cut(s) 2726
PciSI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
PcsI WCGNNNNNNNCGW 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 2130
PfeI GAWTC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 3010
PkrI GCNGC 7 cut(s) 487, 1197, 1252, 1395, 1405, 2953, 2995
PleI GAGTC 3 cut(s) 615, 1564, 1975
PpsI GAGTC 3 cut(s) 615, 1564, 1975
PpuMI RGGWCCY 1 cut(s) 826
Psp124BI GAGCTC 1 cut(s) 93
Psp1406I AACGTT 1 cut(s) 1821
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 6 cut(s) 750, 915, 1510, 2083, 2725, 3010
PspEI GGTNACC 1 cut(s) 1490
PspFI CCCAGC 5 cut(s) 543, 1325, 2548, 2824, 2989
PspGI CCWGG 6 cut(s) 750, 915, 1510, 2083, 2725, 3010
PspPI GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
PspPPI RGGWCCY 1 cut(s) 826
PspXI VCTCGAGB 1 cut(s) 2430
PstI CTGCAG 2 cut(s) 1200, 1395
PstNI CAGNNNCTG 1 cut(s) 2414
PsuI RGATCY 9 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2544, 2866, 2930
RsaI GTAC 9 cut(s) 106, 647, 719, 805, 2163, 2412, 2442, 2764, 2785
RsaNI GTAC 9 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2763, 2784
RseI CAYNNNNRTG 2 cut(s) 930, 1995
SacI GAGCTC 1 cut(s) 93
SapI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
SatI GCNGC 7 cut(s) 486, 1196, 1251, 1394, 1404, 2952, 2994
Sau96I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
SchI GAGTC 3 cut(s) 616, 1564, 1976
ScrFI CCNGG 7 cut(s) 752, 917, 1512, 1631, 2085, 2727, 3012
SduI GDGCHC 7 cut(s) 93, 772, 999, 1135, 1864, 1921, 2437
SfcI CTRYAG 3 cut(s) 1196, 1391, 2970
Sfr274I CTCGAG 2 cut(s) 2430, 2934
SinI GGWCC 6 cut(s) 826, 1960, 2060, 2111, 2627, 2957
SlaI CTCGAG 2 cut(s) 2430, 2934
SmiMI CAYNNNNRTG 2 cut(s) 930, 1995
SmlI CTYRAG 4 cut(s) 649, 2389, 2430, 2934
SmoI CTYRAG 4 cut(s) 649, 2389, 2430, 2934
SspI AATATT 6 cut(s) 678, 779, 840, 1424, 1721, 2206
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 7 cut(s) 750, 915, 1510, 1629, 2083, 2725, 3010
StyI CCWWGG 3 cut(s) 381, 743, 2305
TaaI ACNGT 8 cut(s) 328, 492, 957, 2188, 2243, 2705, 2854, 2920
TaiI ACGT 5 cut(s) 42, 537, 599, 1824, 2526
TaqI TCGA 7 cut(s) 1185, 1317, 1568, 1689, 1978, 2431, 2935
TaqII GACCGA 1 cut(s) 2048
TatI WGTACW 3 cut(s) 717, 2762, 2783
TauI GCSGC 2 cut(s) 1406, 2996
TfiI GAWTC 1 cut(s) 416
TseFI GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
TseI GCWGC 5 cut(s) 485, 1195, 1250, 1393, 2951
Tsp45I GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
TspDTI ATGAA 9 cut(s) 588, 1398, 1788, 2115, 2154, 2210, 2314, 2477, 2699
TspGWI ACGGA 4 cut(s) 13, 980, 1973, 2557
Vha464I CTTAAG 1 cut(s) 2389
VpaK11BI GGWCC 6 cut(s) 826, 1960, 2060, 2111, 2627, 2957
XapI RAATTY 2 cut(s) 1778, 2894
XceI RCATGY 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 2800
XhoI CTCGAG 2 cut(s) 2430, 2934
XmiI GTMKAC 2 cut(s) 658, 2311
XmnI GAANNNNTTC 1 cut(s) 2130
ZraI GACGTC 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.