FvH4_3g30090

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007314
Physical Location & Seq
Forward (+)
23308849 .. 23320839
11991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30090.t2

Sequence Viewer

Length: 3066 bp
ATGGCTTTGTCCGGCATGCGAGGCCTCTCTGTTTTCATCAGCGACATTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTTCGCGTCGATAAGGAGCTCGGCAATGTTCGTACTCGCTTCAAGAACGACAAGGGTTTGACACCGTATGAGAAGAAGAAATATGTTTGGAAAATGCTTTACATTTATATGTTGGGATATGATGTGGATTTTGGTCACATGGAAGCAGTCTCGCTTATATCTGCACCTAAGTATCCAGAAAAGCAGGTTGGGTACATTGTGACATCATGTCTACTCAATGAAAACCATGATTTTCTGAGATTAGCGATAAATACTGTACGAAATGATATTATTGGCCGCAATGAAACTTTCCAATGTTTGGCGTTGACTATGGTTGGGAATATTGGAGGCAGGGAATTTGCGGAGTCATTAGCACCTGATGTACAAAAGTTGCTTATTTCTAGCAGCTACAGACCGCTCGTGAGAAAAAAAGCAGCACTATGTCTTTTGCGCCTGTATAGGAAAAATCCTGATGTTGTCAATATAGATGGCTGGGCCGACCGCATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTGACATCATCTATGAGCCTTCTCGTTGCGCTTGTATCAAATCACCACGATGCGTACTGGAGTTGTCTTCCAAAATGTGTTAAAATACTAGAACGGCTTGCTAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGCATCCCATCGCCCTGGCTCCAGGTCAAGACAATGAGGGTTCTCCAGTATTTTCCCACCGTTGAAGACCCAAATACTAGAAGATCATTGTTTGAGGTCTTGCAACGTATATTGATGGGTACGGATGTTGTGAAAAATGTTAATAAGAACAACGCATCACACGCTGTTCTTTTTGAAGCCCTCGCACTTGTCATGCATCTTGATGCTGAGAAGGAGATGATGTCTCAATGTGTTGCACTTCTGGGAAAATTTATTGCTGTGCGAGAACCAAATATTCGATATCTTGGTTTGGAAAACATGACCCGCATGCTAATGGTTACCGATGTGCAAGATATCATCAAGAGACATCAAGCCCAGATTATCACCTCATTGAAGGATCCAGATGTCAGTATTCGTAGGCGTGCTCTTGACCTGCTTTATGGGATGTGTGATGTTTCAAATGCAAAAGACATAGTTGAAGAACTACTACAGTATCTTAGTACAGCAGACTTCGCAATGCGTGAGGAATTGTCACTAAAGGCTGCCATTCTTGCAGAAAAGTTTGCTCCTGATCTATCATGGTATGTAGATGTGATTCTTCAATTGATTGATAAGGCAGGAGATTTTGTTAGTGATGATATATGGTTCCGTGTTGTGCAATTTGTTACGAATAATGAAGACTTACAGCCTTATGCTGCAGCAAAAGCCAGAGAGTATCTTGACAAACCTGCTATACATGAGACAATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGCCACCTTTTAGCCAGACGGCCTGGGTGTAGTCCAAAGGAATTATTTGCCGTCATACATGAGAAGCTTCCTACCGTATCCACTTCTACAATACCTATACTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAAGAACTACAGAATCAGATTTGGGCAATATTCAGCAAATACGAGAGTTGCATTGATGTTGAAATACAACAACGAGCTGCTGAATACCTTGCCTTAAGTAGGAGAGGTGAAGCTCTTGTTGATATATTGGCTGAAATGCCTAAATTCCCCGAGCGGCAGTCTGCATTGATCAAAAAGGCTGAGGATACTGAAATTGATACCGCAGAACAAAGTGCTATCAAGTTGCGGGCCCAGCAGCAAACATCAAATGCTTTGGTTGTGACCGACCAATGTCCTGGTAATGGTACCCCGCCTGCCAACCACCAGCTTGGTCTTGTGAAGATACCCACCACGAGCAGTAACGTGGATTACAATTCAACCGATCAAGGGTTGTCTCAGGAAAATGGTAATTTGAGTAAAGCTGATCCTCAAACTCCTTCACCGGATCTCCTTGGTGACCTCTTGGGTCCGCTGGCTATTGAAGGCCCTCCTGGTACCACTGTTCAATCACATCAGAATGTAATCCCTGGATCTGGTGGTGATCCAACTGCAGCGGATGCTACGGCCATAGTACCTGTTGGCGAGGAGCCAAATTCTGTTCAGCCTATAGGAAATATTGCTGAAAGATTTCAGGCCTTGTGCTTGAAAGATAGTGGTGTGCTGTATGAAGATCCTAATATTCAGATCGGAGTTAAAGCAGAGTGGCGCTTACATCAGGGGTGTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGCATCAGTTCAGGCTGTAATATTGCCTCCCTCCCACTTCAAAATGGAGCTTTCACTAGTACCAGATACTATTCCTCCTAGGGCACAGGTACAATGCCCCCTTGAAGTTGTGAATCTGCGGCCAAGTAGGGATGTAGCAGTTCTTGACTTCTCTTACAAGTTCGGTCACAATATGGTCAATGTCAAGCTTCGTCTCCCAGCTGTCTTGAATAAATTTCTTCAACCCATACCAGTGTCTGCTGAAGAGTTTTTTCCTCCGTGGAGATCACTCTCAGGACCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGGGTCAAACCACTGCCACTTGCCGAAATGGCAAATCTAATTAACAGCTTCCGGTTGATGGTTTGTCCAGGACTTGATCCCAATCCAAATAATCTTGTTGCAAGCACAACGTTTTACTCAGAAAGTACAAGGGCAATGGTTTGCTTGGCTAGAATAGAAACAGATCCAGCAGACAGAACCCAGTTGCGTATGACGGTTGCTTCAGGGGATCCTACGTTAACATTTGAGTTGAAGGAGTTCATCAAGGAACAAATAGTTAACATTCCTGTAGCCCCTCGTGCACCTGGACCAGTGGCACCAGCACCTCCAGTAGCTCAACCGACCAGTCCAGCTGCAGCATTAACAGATCCTGGTGCTCTGCTGGCCGGTCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1022

Amino Acids

113.46

Weight (kDa)

5.87

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 1.4e-116 Adaptin N terminal region
Cnd1 PF12717 125 - 263 1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 758 - 854 1.4e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 866 - 947 5.4e-11 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 253, 1155, 1450
Acc65I GGTACC 2 cut(s) 1925, 2114
AccB1I GGYRCC 3 cut(s) 1925, 2114, 2983
AccB7I CCANNNNNTGG 1 cut(s) 376
AccBSI CCGCTC 2 cut(s) 475, 1795
AccI GTMKAC 1 cut(s) 289
AccII CGCG 1 cut(s) 84
AclI AACGTT 1 cut(s) 2798
AcoI YGGCCR 5 cut(s) 352, 1495, 2184, 2501, 3051
AcsI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2212, 2594
AcuI CTGAAG 3 cut(s) 2643, 2687, 2874
AfiI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
AflII CTTAAG 1 cut(s) 1735
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 2437
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
Alw21I GWGCWC 4 cut(s) 99, 1141, 2971, 3046
Alw26I GTCTC 6 cut(s) 232, 963, 1072, 1448, 2019, 2579
Alw44I GTGCAC 1 cut(s) 2967
AlwNI CAGNNNCTG 2 cut(s) 2618, 3038
Ama87I CYCGRG 1 cut(s) 1790
ApaI GGGCCC 1 cut(s) 1873
ApaLI GTGCAC 1 cut(s) 2967
ApoI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2212, 2594
Asp700I GAANNNNTTC 2 cut(s) 2247, 2924
Asp718I GGTACC 2 cut(s) 1925, 2114
AspA2I CCTAGG 1 cut(s) 2459
AspLEI GCGC 3 cut(s) 510, 628, 2328
AspS9I GGNCC 7 cut(s) 552, 1869, 1870, 2087, 2105, 2657, 2975
AsuHPI GGTGA 7 cut(s) 632, 1090, 1760, 2052, 2087, 2171, 2367
AvaI CYCGRG 1 cut(s) 1790
AvaII GGWCC 3 cut(s) 2087, 2657, 2975
AvrII CCTAGG 1 cut(s) 2459
BaeGI GKGCMC 3 cut(s) 1873, 2467, 2971
BaeI ACNNNNGTAYC 8 cut(s) 233, 266, 846, 879, 1190, 1223, 2106, 2139
BalI TGGCCA 1 cut(s) 1497
BamHI GGATCC 2 cut(s) 1111, 2896
BanI GGYRCC 3 cut(s) 1925, 2114, 2983
BanII GRGCYC 2 cut(s) 99, 1873
BarI GAAGNNNNNNTAC 2 cut(s) 1185, 1217
BauI CACGAG 3 cut(s) 476, 1972, 2964
BbsI GAAGAC 3 cut(s) 656, 807, 1398
Bbv12I GWGCWC 4 cut(s) 99, 1141, 2971, 3046
BbvCI CCTCAGC 1 cut(s) 1821
BccI CCATC 4 cut(s) 539, 751, 844, 2740
BceAI ACGGC 4 cut(s) 707, 1529, 1529, 2199
BciVI GTATCC 3 cut(s) 261, 1582, 1819
BclI TGATCA 1 cut(s) 1809
BcoDI GTCTC 6 cut(s) 232, 963, 1072, 1448, 2019, 2579
BcuI ACTAGT 1 cut(s) 2437
BfaI CTAG 7 cut(s) 459, 686, 699, 813, 2438, 2460, 2838
BfmI CTRYAG 8 cut(s) 466, 1202, 1410, 1649, 2169, 2226, 2955, 3021
BfoI RGCGCY 1 cut(s) 2329
BfrI CTTAAG 1 cut(s) 1735
BfuAI ACCTGC 3 cut(s) 253, 1155, 1450
BfuI GTATCC 3 cut(s) 261, 1582, 1819
BglI GCCNNNNNGGC 1 cut(s) 2717
BglII AGATCT 1 cut(s) 1614
BlnI CCTAGG 1 cut(s) 2459
Bme18I GGWCC 3 cut(s) 2087, 2657, 2975
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 1790
BmgT120I GGNCC 7 cut(s) 552, 1869, 1870, 2087, 2105, 2657, 2975
BmrI ACTGGG 1 cut(s) 2863
BmsI GCATC 7 cut(s) 637, 747, 899, 928, 940, 2167, 2393
BmuI ACTGGG 1 cut(s) 2863
BoxI GACNNNNGTC 1 cut(s) 1911
BpiI GAAGAC 3 cut(s) 656, 807, 1398
BpmI CTGGAG 5 cut(s) 676, 740, 764, 1620, 2979
Bpu10I CCTNAGC 1 cut(s) 1821
BsaBI GATNNNNATC 1 cut(s) 2769
BsaJI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2639
BsaWI WCCGGW 2 cut(s) 2062, 2739
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
Bse118I RCCGGY 1 cut(s) 3053
Bse1I ACTGG 7 cut(s) 659, 781, 2612, 2869, 2978, 2996, 3012
Bse3DI GCAATG 4 cut(s) 109, 364, 1236, 2829
Bse8I GATNNNNATC 1 cut(s) 2769
BseDI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2639
BseGI GGATG 5 cut(s) 738, 865, 1164, 2182, 2518
BseJI GATNNNNATC 1 cut(s) 2769
BseLI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
BseMI GCAATG 4 cut(s) 109, 364, 1236, 2829
BseMII CTCAG 6 cut(s) 305, 933, 1812, 2030, 2667, 2820
BseNI ACTGG 7 cut(s) 659, 781, 2612, 2869, 2978, 2996, 3012
BseRI GAGGAG 1 cut(s) 2219
BseSI GKGCMC 3 cut(s) 1873, 2467, 2971
BseYI CCCAGC 3 cut(s) 549, 1872, 2578
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 559
BshNI GGYRCC 3 cut(s) 1925, 2114, 2983
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 4 cut(s) 99, 1141, 2971, 3046
BsiHKCI CYCGRG 1 cut(s) 1790
BsiSI CCGG 4 cut(s) 12, 2063, 2740, 3054
BslI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
BsmAI GTCTC 6 cut(s) 232, 963, 1072, 1448, 2019, 2579
BsmBI CGTCTC 1 cut(s) 2579
BsoBI CYCGRG 1 cut(s) 1790
Bsp120I GGGCCC 1 cut(s) 1869
Bsp1286I GDGCHC 6 cut(s) 99, 1141, 1873, 2467, 2971, 3046
Bsp1407I TGTACA 1 cut(s) 439
BspCNI CTCAG 6 cut(s) 306, 934, 1813, 2029, 2666, 2819
BspFNI CGCG 1 cut(s) 84
BspMAI CTGCAG 3 cut(s) 1414, 2173, 3025
BspMI ACCTGC 3 cut(s) 253, 1155, 1450
BspT107I GGYRCC 3 cut(s) 1925, 2114, 2983
BspTI CTTAAG 1 cut(s) 1735
BsrBI CCGCTC 2 cut(s) 475, 1795
BsrDI GCAATG 4 cut(s) 109, 364, 1236, 2829
BsrFI RCCGGY 1 cut(s) 3053
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 7 cut(s) 659, 781, 2612, 2869, 2978, 2996, 3012
BssAI RCCGGY 1 cut(s) 3053
BssECI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2639
BssSI CACGAG 3 cut(s) 476, 1972, 2964
BssT1I CCWWGG 2 cut(s) 2071, 2459
Bst2BI CACGAG 3 cut(s) 476, 1972, 2964
Bst4CI ACNGT 7 cut(s) 144, 334, 796, 1206, 1570, 2122, 2884
Bst6I CTCTTC 1 cut(s) 2619
BstAFI CTTAAG 1 cut(s) 1735
BstAPI GCANNNNNTGC 1 cut(s) 2177
BstAUI TGTACA 1 cut(s) 439
BstC8I GCNNGC 9 cut(s) 17, 696, 1043, 1137, 1869, 1935, 2094, 2791, 3051
BstDEI CTNAG 9 cut(s) 246, 314, 942, 1211, 1611, 1821, 2016, 2653, 2806
BstDSI CCRYGG 1 cut(s) 2639
BstEII GGTNACC 2 cut(s) 1051, 2075
BstENI CCTNNNNNAGG 1 cut(s) 1738
BstF5I GGATG 5 cut(s) 738, 865, 1164, 2182, 2518
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 2329
BstHHI GCGC 3 cut(s) 510, 628, 2328
BstMAI GTCTC 6 cut(s) 232, 963, 1072, 1448, 2019, 2579
BstMCI CGRYCG 1 cut(s) 559
BstNSI RCATGY 2 cut(s) 19, 1045
BstPAI GACNNNNGTC 1 cut(s) 1911
BstPI GGTNACC 2 cut(s) 1051, 2075
BstSFI CTRYAG 8 cut(s) 466, 1202, 1410, 1649, 2169, 2226, 2955, 3021
BstSLI GKGCMC 3 cut(s) 1873, 2467, 2971
BstUI CGCG 1 cut(s) 84
BstV2I GAAGAC 3 cut(s) 656, 807, 1398
BstX2I RGATCY 8 cut(s) 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
BstXI CCANNNNNNTGG 3 cut(s) 750, 1916, 1949
BstYI RGATCY 8 cut(s) 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
BsuI GTATCC 3 cut(s) 261, 1582, 1819
BtgI CCRYGG 1 cut(s) 2639
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 5 cut(s) 738, 865, 1164, 2182, 2518
BtsI GCAGTG 1 cut(s) 2699
BtsIMutI CAGTG 5 cut(s) 1477, 2118, 2619, 2699, 2985
BveI ACCTGC 3 cut(s) 253, 1155, 1450
Cac8I GCNNGC 9 cut(s) 17, 696, 1043, 1137, 1869, 1935, 2094, 2791, 3051
CaiI CAGNNNCTG 2 cut(s) 2618, 3038
CfoI GCGC 3 cut(s) 510, 628, 2328
Cfr10I RCCGGY 1 cut(s) 3053
Cfr13I GGNCC 7 cut(s) 552, 1869, 1870, 2087, 2105, 2657, 2975
CseI GACGC 1 cut(s) 73
DdeI CTNAG 9 cut(s) 246, 314, 942, 1211, 1611, 1821, 2016, 2653, 2806
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 5 cut(s) 352, 1495, 2184, 2501, 3051
Eam1104I CTCTTC 1 cut(s) 2619
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 2619
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 2071, 2459
Eco147I AGGCCT 2 cut(s) 24, 2255
Eco24I GRGCYC 2 cut(s) 99, 1873
Eco32I GATATC 2 cut(s) 1016, 1069
Eco47I GGWCC 3 cut(s) 2087, 2657, 2975
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 2643, 2687, 2874
Eco88I CYCGRG 1 cut(s) 1790
Eco91I GGTNACC 2 cut(s) 1051, 2075
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 1738
EcoO109I RGGNCCY 1 cut(s) 2105
EcoO65I GGTNACC 2 cut(s) 1051, 2075
EcoRV GATATC 2 cut(s) 1016, 1069
EcoT14I CCWWGG 2 cut(s) 2071, 2459
EcoT22I ATGCAT 1 cut(s) 933
EcoT38I GRGCYC 2 cut(s) 99, 1873
ErhI CCWWGG 2 cut(s) 2071, 2459
Esp3I CGTCTC 1 cut(s) 2579
FauI CCCGC 3 cut(s) 1046, 1860, 1938
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 289
FokI GGATG 5 cut(s) 725, 872, 1171, 2189, 2525
FriOI GRGCYC 2 cut(s) 99, 1873
FspBI CTAG 7 cut(s) 459, 686, 699, 813, 2438, 2460, 2838
GlaI GCGC 3 cut(s) 509, 627, 2327
GsaI CCCAGC 3 cut(s) 553, 1876, 2582
GsuI CTGGAG 5 cut(s) 676, 740, 764, 1620, 2979
HaeII RGCGCY 1 cut(s) 2329
HapII CCGG 4 cut(s) 12, 2063, 2740, 3054
HgaI GACGC 1 cut(s) 73
HhaI GCGC 3 cut(s) 510, 628, 2328
Hin6I GCGC 3 cut(s) 508, 626, 2326
HinP1I GCGC 3 cut(s) 508, 626, 2326
HincII GTYRAC 3 cut(s) 384, 2907, 2947
HindII GTYRAC 3 cut(s) 384, 2907, 2947
HindIII AAGCTT 3 cut(s) 1559, 2567, 2669
HinfI GANTC 4 cut(s) 422, 1309, 1654, 2494
HpaI GTTAAC 2 cut(s) 2907, 2947
HpaII CCGG 4 cut(s) 12, 2063, 2740, 3054
HphI GGTGA 7 cut(s) 632, 1090, 1760, 2052, 2087, 2171, 2367
Hpy166II GTNNAC 6 cut(s) 290, 384, 726, 2907, 2947, 2969
Hpy188I TCNGA 8 cut(s) 51, 315, 1659, 2136, 2304, 2309, 2667, 2809
Hpy8I GTNNAC 6 cut(s) 290, 384, 726, 2907, 2947, 2969
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 6 cut(s) 626, 940, 1102, 2067, 2096, 2914
HpyCH4III ACNGT 7 cut(s) 144, 334, 796, 1206, 1570, 2122, 2884
HpyCH4IV ACGT 5 cut(s) 583, 841, 1983, 2798, 2903
HpyF3I CTNAG 9 cut(s) 246, 314, 942, 1211, 1611, 1821, 2016, 2653, 2806
HpySE526I ACGT 5 cut(s) 583, 841, 1983, 2798, 2903
HspAI GCGC 3 cut(s) 508, 626, 2326
KpnI GGTACC 2 cut(s) 1929, 2118
Ksp22I TGATCA 1 cut(s) 1809
KspAI GTTAAC 2 cut(s) 2907, 2947
LmnI GCTCC 5 cut(s) 94, 759, 1285, 2206, 2428
LweI GCATC 7 cut(s) 637, 747, 899, 928, 940, 2167, 2393
MaeI CTAG 7 cut(s) 459, 686, 699, 813, 2438, 2460, 2838
MaeII ACGT 5 cut(s) 583, 841, 1983, 2798, 2903
MaeIII GTNAC 9 cut(s) 212, 277, 1051, 1245, 1378, 1900, 1979, 2075, 2546
MbiI CCGCTC 2 cut(s) 475, 1795
MfeI CAATTG 1 cut(s) 1316
MflI RGATCY 8 cut(s) 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
MhlI GDGCHC 6 cut(s) 99, 1141, 1873, 2467, 2971, 3046
MlsI TGGCCA 1 cut(s) 1497
MluNI TGGCCA 1 cut(s) 1497
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 2189
Mox20I TGGCCA 1 cut(s) 1497
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 2 cut(s) 2247, 2924
MscI TGGCCA 1 cut(s) 1497
MseI TTAA 9 cut(s) 678, 876, 1464, 1736, 2313, 2730, 2906, 2946, 3029
MslI CAYNNNNRTG 6 cut(s) 185, 645, 936, 1046, 2136, 2612
Msp20I TGGCCA 1 cut(s) 1497
MspA1I CMGCKG 4 cut(s) 2092, 2174, 2582, 3020
MspCI CTTAAG 1 cut(s) 1735
MspI CCGG 4 cut(s) 12, 2063, 2740, 3054
MunI CAATTG 1 cut(s) 1316
MvnI CGCG 1 cut(s) 84
NdeI CATATG 1 cut(s) 1606
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 6 cut(s) 212, 277, 1245, 1900, 2075, 2546
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 2 cut(s) 19, 1045
PaeI GCATGC 2 cut(s) 19, 1045
PceI AGGCCT 2 cut(s) 24, 2255
PdmI GAANNNNTTC 2 cut(s) 2247, 2924
PfeI GAWTC 3 cut(s) 1309, 1654, 2494
PflMI CCANNNNNTGG 1 cut(s) 376
PfoI TCCNGGA 1 cut(s) 2755
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1911
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 2798
PspEI GGTNACC 2 cut(s) 1051, 2075
PspFI CCCAGC 3 cut(s) 549, 1872, 2578
PspOMI GGGCCC 1 cut(s) 1869
PspPI GGNCC 7 cut(s) 552, 1869, 1870, 2087, 2105, 2657, 2975
PstI CTGCAG 3 cut(s) 1414, 2173, 3025
PstNI CAGNNNCTG 2 cut(s) 2618, 3038
PsuI RGATCY 8 cut(s) 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
PvuII CAGCTG 2 cut(s) 2582, 3020
RseI CAYNNNNRTG 6 cut(s) 185, 645, 936, 1046, 2136, 2612
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 9 cut(s) 678, 876, 1464, 1736, 2313, 2730, 2906, 2946, 3029
Sau96I GGNCC 7 cut(s) 552, 1869, 1870, 2087, 2105, 2657, 2975
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 6 cut(s) 99, 1141, 1873, 2467, 2971, 3046
SfaNI GCATC 7 cut(s) 637, 747, 899, 928, 940, 2167, 2393
SfcI CTRYAG 8 cut(s) 466, 1202, 1410, 1649, 2169, 2226, 2955, 3021
SinI GGWCC 3 cut(s) 2087, 2657, 2975
SmiMI CAYNNNNRTG 6 cut(s) 185, 645, 936, 1046, 2136, 2612
SmlI CTYRAG 1 cut(s) 1735
SmoI CTYRAG 1 cut(s) 1735
SpeI ACTAGT 1 cut(s) 2437
SphI GCATGC 2 cut(s) 19, 1045
SseBI AGGCCT 2 cut(s) 24, 2255
SspI AATATT 6 cut(s) 400, 1009, 1671, 2236, 2299, 2403
SspMI CTAG 7 cut(s) 459, 686, 699, 813, 2438, 2460, 2838
SstI GAGCTC 1 cut(s) 99
StuI AGGCCT 2 cut(s) 24, 2255
StyI CCWWGG 2 cut(s) 2071, 2459
TaaI ACNGT 7 cut(s) 144, 334, 796, 1206, 1570, 2122, 2884
TaiI ACGT 5 cut(s) 586, 844, 1986, 2801, 2906
TaqI TCGA 2 cut(s) 87, 1012
TaqII GACCGA 2 cut(s) 1919, 2534
TatI WGTACW 4 cut(s) 439, 723, 1214, 2813
TauI GCSGC 3 cut(s) 357, 1798, 2503
TfiI GAWTC 3 cut(s) 1309, 1654, 2494
Tru1I TTAA 9 cut(s) 678, 876, 1464, 1736, 2313, 2730, 2906, 2946, 3029
Tru9I TTAA 9 cut(s) 678, 876, 1464, 1736, 2313, 2730, 2906, 2946, 3029
TscAI CASTG 5 cut(s) 1477, 2125, 2619, 2706, 2985
TseFI GTSAC 6 cut(s) 212, 277, 1245, 1900, 2075, 2546
Tsp45I GTSAC 6 cut(s) 212, 277, 1245, 1900, 2075, 2546
TspDTI ATGAA 7 cut(s) 25, 312, 375, 594, 1404, 2301, 2917
TspGWI ACGGA 3 cut(s) 872, 1352, 2628
TspRI CASTG 5 cut(s) 1477, 2125, 2619, 2706, 2985
Van91I CCANNNNNTGG 1 cut(s) 376
Vha464I CTTAAG 1 cut(s) 1735
VneI GTGCAC 1 cut(s) 2967
VpaK11BI GGWCC 3 cut(s) 2087, 2657, 2975
XagI CCTNNNNNAGG 1 cut(s) 1738
XapI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2212, 2594
XceI RCATGY 2 cut(s) 19, 1045
XmaJI CCTAGG 1 cut(s) 2459
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 2247, 2924
XspI CTAG 7 cut(s) 459, 686, 699, 813, 2438, 2460, 2838
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.