Rorug05G0294700

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007314
Physical Location & Seq
Reverse (-)
33993546 .. 33998442
4897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0294700.1

Sequence Viewer

Length: 1251 bp
ATGGCTACTTTGGCGAACCCGAAGGAGCTAATTTCGACGACGTCGTCCTCAATTCCCACCGCATTTCACCACCACAACGCATTGACTTCCCCCAGAACATGCATTCCTCTGGGTACCAAAAGAAGGAGCGCAGCCAAACGACATCGTTTCGGGTCGGTCAGCTGCTCCTTCGCGCCCATGGAAACGGCCAAGATTAAGGTCGTTGGGGTCGGCGGCGGCGGCAACAATGCCGTTAATCGCATGATTGGCAGCGGTTTACATGGTGTTGATTTCTATGCGATAAACACGGATGCTCAAGCACTGTTACAGTCAGCTGCAGAGAACCCGCTTCAAATTGGAGAGCTTTTGACTCGTGGACTAGGTACTGGTGGGAATCCACTTTTGGGGGAACAAGCTGCTGAGGAATCAAAAGAGGCCATTGCGGGTGCTCTTAAAGGCTCAGATCTCGTATTTATAACAGCTGGTATGGGTGGAGGCACAGGGTCTGGTGCTGCCCCTGTTGTTGCACAAATATCTAGAGAGGCAGGCTATTTGACTGTTGGTGTGGTTACCTACCCTTTCAGCTTTGAAGGGCGTAAAAGATCAGTGCAGGCATTGGAGGCTATTGAAAAGTTGCAGAAAAATGTTGACACTCTTATAGTGATACCCAATGACCGCCTGCTTGATATTGCTGATGAGCAGACACCCCTTCAGGATGCTTTCCTTCTTGCTGATGATGTTTTGCGTCAAGGAGTGCAAGGAATTTCTGACATAATCACAATACCTGGACTGGTAAATGTGGATTTTGCTGATGTAAAGGCAGTCATGAAAGACTCAGGAACCGCAATGCTTGGAGTAGGGGTCTCCTCCAGCAAAAATCGTGCAGAAGAAGCAGCTGAACAGGCGACTTTGGCTCCGCTAATTGGGTCTTCAATACAATCAGCCACTGGAGTAGTGTATAATATCACTGGAGGAAAGGACATAACCCTGCAGGAAGTGAACAAAGTGTCTCAGGTTGTAACAAGTTTGGCGGATCCTTCTGCTAACATAATATTTGGGGCTGTTGTGGATGACCGCTACACCGGAGAGATTCATGTGACTATTATTGCAACAGGCTTTTCACAATCATTTCAGAAGACACTACTAACAGACCCCAAAGCAGCAAGGCTGGTTGACAAAGTTGCAGCAGGTCAAGAAACCAGGGGAATCCCCCTTCCCCTCAAGTCCTCCACCACTCCATCAAGACCGGCTACAAGAAAGCTTTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

416

Amino Acids

43.38

Weight (kDa)

6.62

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 64 - 224 5.8e-49 Tubulin/FtsZ family, GTPase domain
FtsZ_C PF12327 273 - 366 6.2e-30 FtsZ family, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AasI GACNNNNNNGTC 1 cut(s) 43
AatII GACGTC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 1157
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 113
AccII CGCG 1 cut(s) 173
AclWI GGATC 2 cut(s) 1007, 1020
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 741
AcuI CTGAAG 1 cut(s) 674
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 115, 364
AfiI CCNNNNNNNGG 3 cut(s) 123, 383, 902
AgsI TTSAA 4 cut(s) 332, 569, 608, 912
AjnI CCWGG 2 cut(s) 763, 1178
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
AluBI AGCT 9 cut(s) 28, 162, 314, 343, 395, 461, 564, 875, 1240
AluI AGCT 9 cut(s) 28, 162, 314, 343, 395, 461, 564, 875, 1240
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 847, 993
AlwI GGATC 2 cut(s) 1007, 1020
AlwNI CAGNNNCTG 2 cut(s) 485, 926
AoxI GGCC 2 cut(s) 186, 414
ApeKI GCWGC 9 cut(s) 131, 162, 249, 314, 395, 491, 872, 1139, 1163
ApoI RAATTY 1 cut(s) 741
Asp718I GGTACC 1 cut(s) 113
AspLEI GCGC 2 cut(s) 131, 175
AsuHPI GGTGA 1 cut(s) 59
BamHI GGATCC 1 cut(s) 1012
BanI GGYRCC 1 cut(s) 113
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 2 cut(s) 900, 1121
Bbv12I GWGCWC 1 cut(s) 430
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 9 cut(s) 143, 149, 261, 301, 382, 478, 884, 1151, 1175
BccI CCATC 1 cut(s) 1225
BceAI ACGGC 2 cut(s) 201, 215
BciT130I CCWGG 2 cut(s) 765, 1180
BcoDI GTCTC 2 cut(s) 847, 993
BfaI CTAG 2 cut(s) 359, 516
BfmI CTRYAG 2 cut(s) 315, 968
BfuAI ACCTGC 1 cut(s) 1157
BglII AGATCT 1 cut(s) 442
Bme1390I CCNGG 2 cut(s) 765, 1180
BmiI GGNNCC 4 cut(s) 115, 820, 894, 1014
BmrFI CCNGG 2 cut(s) 765, 1180
BmsI GCATC 2 cut(s) 280, 685
BpiI GAAGAC 2 cut(s) 900, 1121
BpmI CTGGAG 3 cut(s) 832, 948, 969
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 2 cut(s) 279, 1184
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 847
BsaJI CCNNGG 2 cut(s) 177, 1179
BsaWI WCCGGW 1 cut(s) 1061
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 877, 907
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 383, 902
Bse118I RCCGGY 1 cut(s) 1225
Bse1I ACTGG 4 cut(s) 370, 774, 931, 952
Bse3DI GCAATG 2 cut(s) 417, 831
BseBI CCWGG 2 cut(s) 765, 1180
BseDI CCNNGG 2 cut(s) 177, 1179
BseGI GGATG 3 cut(s) 295, 700, 1054
BseLI CCNNNNNNNGG 3 cut(s) 123, 383, 902
BseMI GCAATG 2 cut(s) 417, 831
BseMII CTCAG 4 cut(s) 390, 453, 828, 1004
BseNI ACTGG 4 cut(s) 370, 774, 931, 952
BseRI GAGGAG 1 cut(s) 835
BseXI GCAGC 9 cut(s) 143, 149, 261, 301, 382, 478, 884, 1151, 1175
BsgI GTGCAG 2 cut(s) 608, 882
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 2 cut(s) 188, 416
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 2 cut(s) 1062, 1226
BslI CCNNNNNNNGG 3 cut(s) 123, 383, 902
BsmAI GTCTC 2 cut(s) 847, 993
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 2 cut(s) 188, 416
Bso31I GGTCTC 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 3 cut(s) 442, 581, 1012
Bsp19I CCATGG 1 cut(s) 177
BspANI GGCC 2 cut(s) 188, 416
BspCNI CTCAG 4 cut(s) 391, 452, 827, 1003
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 4 cut(s) 115, 820, 894, 1014
BspMAI CTGCAG 2 cut(s) 319, 972
BspMI ACCTGC 1 cut(s) 1157
BspPI GGATC 2 cut(s) 1007, 1020
BspT107I GGYRCC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 417, 831
BsrFI RCCGGY 1 cut(s) 1225
BsrI ACTGG 4 cut(s) 370, 774, 931, 952
BssAI RCCGGY 1 cut(s) 1225
BssECI CCNNGG 2 cut(s) 177, 1179
BssMI GATC 3 cut(s) 442, 581, 1012
BssNI GRCGYC 1 cut(s) 41
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 765, 1180
Bst4CI ACNGT 3 cut(s) 303, 309, 538
BstACI GRCGYC 1 cut(s) 41
BstC8I GCNNGC 3 cut(s) 526, 591, 659
BstDEI CTNAG 4 cut(s) 399, 439, 814, 990
BstDSI CCRYGG 1 cut(s) 177
BstEII GGTNACC 1 cut(s) 547
BstF5I GGATG 3 cut(s) 295, 700, 1054
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 2 cut(s) 131, 175
BstKTI GATC 3 cut(s) 445, 584, 1015
BstMAI GTCTC 2 cut(s) 847, 993
BstMBI GATC 3 cut(s) 442, 581, 1012
BstMWI GCNNNNNNNGC 7 cut(s) 11, 219, 246, 599, 869, 881, 890
BstNI CCWGG 2 cut(s) 765, 1180
BstNSI RCATGY 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 547
BstSCI CCNGG 2 cut(s) 763, 1178
BstSFI CTRYAG 2 cut(s) 315, 968
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 9 cut(s) 143, 149, 261, 301, 382, 478, 884, 1151, 1175
BstV2I GAAGAC 2 cut(s) 900, 1121
BstX2I RGATCY 2 cut(s) 442, 1012
BstYI RGATCY 2 cut(s) 442, 1012
BsuRI GGCC 2 cut(s) 188, 416
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 295, 700, 1054
BtsIMutI CAGTG 4 cut(s) 299, 591, 924, 945
BveI ACCTGC 1 cut(s) 1157
Cac8I GCNNGC 3 cut(s) 526, 591, 659
CaiI CAGNNNCTG 2 cut(s) 485, 926
CciI TCATGA 1 cut(s) 804
CfoI GCGC 2 cut(s) 131, 175
Cfr10I RCCGGY 1 cut(s) 1225
CseI GACGC 1 cut(s) 713
Csp6I GTAC 2 cut(s) 114, 363
CviAII CATG 6 cut(s) 99, 178, 241, 260, 805, 1073
CviQI GTAC 2 cut(s) 114, 363
DdeI CTNAG 4 cut(s) 399, 439, 814, 990
DpnI GATC 3 cut(s) 444, 583, 1014
DpnII GATC 3 cut(s) 442, 581, 1012
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 186
EciI GGCGGA 1 cut(s) 1025
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 674
Eco91I GGTNACC 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 763, 1178
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 6 cut(s) 102, 181, 244, 263, 808, 1076
FalI AAGNNNNNCTT 1 cut(s) 1229
FatI CATG 6 cut(s) 98, 177, 240, 259, 804, 1072
FauI CCCGC 2 cut(s) 333, 415
FokI GGATG 3 cut(s) 302, 707, 1061
FspBI CTAG 2 cut(s) 359, 516
GlaI GCGC 2 cut(s) 130, 174
GsuI CTGGAG 3 cut(s) 832, 948, 969
HaeIII GGCC 2 cut(s) 188, 416
HapII CCGG 2 cut(s) 1062, 1226
HgaI GACGC 1 cut(s) 713
HhaI GCGC 2 cut(s) 131, 175
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 6 cut(s) 102, 181, 244, 263, 808, 1076
Hin6I GCGC 2 cut(s) 129, 173
HinP1I GCGC 2 cut(s) 129, 173
HincII GTYRAC 2 cut(s) 628, 1153
HindII GTYRAC 2 cut(s) 628, 1153
HindIII AAGCTT 1 cut(s) 1238
HinfI GANTC 6 cut(s) 349, 373, 404, 812, 1069, 1185
HpaII CCGG 2 cut(s) 1062, 1226
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 5 cut(s) 257, 356, 628, 979, 1153
Hpy188I TCNGA 3 cut(s) 442, 748, 1113
Hpy188III TCNNGA 6 cut(s) 516, 692, 805, 816, 1172, 1221
Hpy8I GTNNAC 5 cut(s) 257, 356, 628, 979, 1153
Hpy99I CGWCG 3 cut(s) 40, 43, 46
HpyAV CCTTC 8 cut(s) 16, 117, 178, 563, 698, 713, 1026, 1202
HpyCH4III ACNGT 3 cut(s) 303, 309, 538
HpyCH4IV ACGT 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 219, 246, 599, 869, 881, 890
HpyF3I CTNAG 4 cut(s) 399, 439, 814, 990
HpySE526I ACGT 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 6 cut(s) 102, 181, 244, 263, 808, 1076
HspAI GCGC 2 cut(s) 129, 173
KpnI GGTACC 1 cut(s) 117
Kzo9I GATC 3 cut(s) 442, 581, 1012
LmnI GCTCC 4 cut(s) 25, 126, 170, 898
Lsp1109I GCAGC 9 cut(s) 143, 149, 261, 301, 382, 478, 884, 1151, 1175
LweI GCATC 2 cut(s) 280, 685
MaeI CTAG 2 cut(s) 359, 516
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 4 cut(s) 303, 547, 997, 1075
MalI GATC 3 cut(s) 444, 583, 1014
MboI GATC 3 cut(s) 442, 581, 1012
MboII GAAGA 3 cut(s) 878, 900, 1126
MflI RGATCY 2 cut(s) 442, 1012
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 5 cut(s) 30, 51, 333, 741, 900
MlyI GAGTC 2 cut(s) 343, 806
Mph1103I ATGCAT 1 cut(s) 104
MseI TTAA 4 cut(s) 195, 234, 432, 1249
MspA1I CMGCKG 5 cut(s) 162, 252, 314, 461, 875
MspI CCGG 2 cut(s) 1062, 1226
MspR9I CCNGG 2 cut(s) 765, 1180
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 2 cut(s) 765, 1180
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 7 cut(s) 11, 219, 246, 599, 869, 881, 890
NcoI CCATGG 1 cut(s) 177
NdeII GATC 3 cut(s) 442, 581, 1012
NlaIII CATG 6 cut(s) 102, 181, 244, 263, 808, 1076
NlaIV GGNNCC 4 cut(s) 115, 820, 894, 1014
NmuCI GTSAC 1 cut(s) 1075
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 1 cut(s) 102
PagI TCATGA 1 cut(s) 804
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PctI GAATGC 1 cut(s) 102
PfeI GAWTC 4 cut(s) 373, 404, 1069, 1185
PflFI GACNNNGTC 2 cut(s) 40, 43
PleI GAGTC 2 cut(s) 343, 806
PpsI GAGTC 2 cut(s) 343, 806
PsiI TTATAA 1 cut(s) 455
Psp6I CCWGG 2 cut(s) 763, 1178
PspEI GGTNACC 1 cut(s) 547
PspGI CCWGG 2 cut(s) 763, 1178
PspN4I GGNNCC 4 cut(s) 115, 820, 894, 1014
PstI CTGCAG 2 cut(s) 319, 972
PstNI CAGNNNCTG 2 cut(s) 485, 926
PsuI RGATCY 2 cut(s) 442, 1012
PsyI GACNNNGTC 2 cut(s) 40, 43
PvuII CAGCTG 4 cut(s) 162, 314, 461, 875
RsaI GTAC 2 cut(s) 115, 364
RsaNI GTAC 2 cut(s) 114, 363
SaqAI TTAA 4 cut(s) 195, 234, 432, 1249
Sau3AI GATC 3 cut(s) 442, 581, 1012
SbfI CCTGCAGG 1 cut(s) 972
SchI GAGTC 2 cut(s) 343, 806
ScrFI CCNGG 2 cut(s) 765, 1180
SdaI CCTGCAGG 1 cut(s) 972
SduI GDGCHC 1 cut(s) 430
SfaNI GCATC 2 cut(s) 280, 685
SfcI CTRYAG 2 cut(s) 315, 968
SmlI CTYRAG 2 cut(s) 294, 1199
SmoI CTYRAG 2 cut(s) 294, 1199
Sse8387I CCTGCAGG 1 cut(s) 972
Sse9I AATT 5 cut(s) 30, 51, 333, 741, 900
SspI AATATT 1 cut(s) 1032
SspMI CTAG 2 cut(s) 359, 516
StyD4I CCNGG 2 cut(s) 763, 1178
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 3 cut(s) 303, 309, 538
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 35
TaqII GACCGA 1 cut(s) 145
TasI AATT 5 cut(s) 30, 51, 333, 741, 900
TauI GCSGC 3 cut(s) 216, 219, 222
TfiI GAWTC 4 cut(s) 373, 404, 1069, 1185
Tru1I TTAA 4 cut(s) 195, 234, 432, 1249
Tru9I TTAA 4 cut(s) 195, 234, 432, 1249
TscAI CASTG 4 cut(s) 306, 591, 931, 952
TseFI GTSAC 1 cut(s) 1075
TseI GCWGC 9 cut(s) 131, 162, 249, 314, 395, 491, 872, 1139, 1163
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 2 cut(s) 821, 1061
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 4 cut(s) 306, 591, 931, 952
Tth111I GACNNNGTC 2 cut(s) 40, 43
XapI RAATTY 1 cut(s) 741
XbaI TCTAGA 1 cut(s) 515
XceI RCATGY 1 cut(s) 102
XspI CTAG 2 cut(s) 359, 516
ZraI GACGTC 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.