MD03G1132700.v1.1

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007314
Physical Location & Seq
Forward (+)
13191917 .. 13205498
13582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1132700.v1.1.491

Sequence Viewer

Length: 3060 bp
ATGGCGTTGTCAGGCATGCGAGGCCTCTCTGTTTTCATCAGCGACATTCGGAATTGTCAGAACAAAGAGCAGGAGAGGCTCCGGGTCGATAAGGAGCTGGGGAATGTCCGTACACGCTTCAAAAACGAAAAGGGTTTGACACCTTATGAAAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGCTGGGCTATGATGTGGATTTTGGGCACATGGAAGCAGTTTCTCTAATATCTGCGCCTAAGTATCCAGAAAAGCAGGTTGGGTACATTGTAACAGCATGCTTGCTCAATGAGAACCATGATTTTCTGAGAATAGCGATAAATACGGTGCGCAATGACATCATTGGTCGGAATGAGACTTTCCAATGTTTGGCGTTGACTATGGTTGGGAATATTGGTGGCCGAGAATTTGCGGAGTCATTAGCACCTGATGTTCAAAAATTGCTTATTTCTAGCAGCTACAGACCACTTGTGAGAAAAAAGGCTGCACTATGTTTATTGCGCTTGTATAGAAAAAATCCTGATGTTGTGAATGTAGATGGCTGGGCTGACCGGATGGCACAACTTTTAGATGAACGTGATCTTGGTGTGTTAACATCATCTATGAGCCTTCTTGTTGCCCTTGTATCAAACCACTATGATGCTTACTGGAGTTGTCTGCCAAAATGCGTTAAAATATTAGAACGACTTGCTAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGCATCCCATCTCCCTGGCTTCAGGTCAGGACAATGAGGGCTCTTCAGTATTTTCCAACCGTCGAAGATCCAAATACCAGAAGATCATTGTTTGAGGTCTTACAGCGGATTTTGATGGGTACTGATGTTGTGAAAAATGTTAATAAGAACAATGCATCACATGCTGTTCTCTTTGAAGCCCTCGCTCTTGTCATGCATCTTGATGCTGAAAAGGAGATGATGTCTCAGTGTGTTGCCCTTCTTGGAAAATTTATTGCTGTCCGAGAACCAAATATCCGATATCTTGGTTTGGAAAATATGACCCGCATGTTAATGGTGACTGATGTGCAAGATATCATCAAGAGACATCAGGCTCAGATCATCACCTCATTGAAGGATCCAGATATCAGTATACGTAGGCGTGCTCTAGACTTGCTTTATGGTATGTGTGATGTTTCAAATGCAAAAGATATAGTCGAAGAATTATTGCAGTATCTTAGCACAGCAGATTTTGCCATGCGTGAAGAATTGTCTCTTAAGGCTGCCATTCTTGCAGAAAAGTTTGCCCCTGATCTTTCTTGGTATGTAGATGTGATTCTTCAATTAATTGACAAGGCTGGAGACTTTGTTAGTGATGATATATGGTTTCGTGTTGTGCAATTTGTTACAAATAACGAAGACTTACAGCCTTATGCAGCAGCAAAAGCTAGGGAGTATCTAGACAAACCTGCTATACATGAGACTATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGCCACCTTTTGGCCAGACGGCCTGGGTGCAGTCCAAAGGAATTATTTAGCGTCATACATGAGAAGCTTCCTGCTGTGACGACATCTACGGTACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGATCAGGAACTACAGAATCAGATCTGGTCAATTTTCAACAAATATGAGAGTTGCATTGATGTTGAGATACAACAAAGAGCTGCTGAATACCTTGCCTTAAGTAGGAGAGGTGCAGCTCTGGTGGATATTTTGGCTGAAATGCCTAAATTTCCTGAGCGGCAGTCTGCATTGATCAAGAAGGCCGAAGATACAGAAGTTGATACAGCGGAACAAAGTGCTATCAAGTTGCGGGCCCAGCAGCAAACATCGAGTGCTTTGGTTGTGACCGACCAACGCCCTGCTAATGGGACCCCGCCTGTCAACCAGTTTGATCTTGTCAAGATGCCCAGCATGAGCAGTAATGCGGATCACAATTCAACTGACCAAGGGTCGTCTCAGGAAAATGGTACTTTGAGTAAAGTTGATCCTCAGGCACCTTCAGCAGATATCCTTGGTGATCTATTGGGTCCCATGGCTATTGAAGGCCCTCCCGCTACTGCTGGTCAGCCTCAGCAGAATGTAATCCCTGGATTAGGAGGTGATCCAGATGCCGTGGATGCTTCAGCAATTGTGCCTGTTGGCGAGGAGCAAAATTCTGTTCAGCCAATAGGAAATATTGCTGAAAGGTTTCATGCTCTCTGCTTGAAAGATAGTGGTGTTCTATACGAAGATCCTAATATTCAGATTGGAATTAAAGCCGAGTGGCGGCTACATCAAGGATGTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGTGTCAGTTCAGGCTATAATATTACCTCCCTCTCATTTGAAAATGGAGCTCTCACTAGTACCGGATACTATTCCTCCCAGGGCACAGGTACAATGCCCGCTTGAACTTGTGAATCTCAGACCTAGCAGGGATGTAGCAGTTCTTGATTTTTCCTACAAGTTTGGTAACAATATGGTCAATGTCAAGCTTCGCCTCCCAGCTGTCTTAAATAAATTTCTTCAGCCTATTCCAGTGTCTGCTGAAGAGTTCTTTCCTCAGTGGAGATCACTTTCAGGGCCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGTGTCAAACCAATGCCACTTGTAGAAATGGCGAATCTACTTAACAGTTTTCGGTTGATGGTTTGTCCGGCACTTGACCCAAATCCAAATAATCTTGTTGCAAGTACAACGTTTTACTCAGAAAGTACACGGGCCATGCTTTGCTTGGTTAGAATAGAAACAGATCCAGCAGACAGAACACAGTTGCGTATGACAGTTGCTTCAGGGGACCCTACATTAACGCTCGAGTTGAAGGAGTTCATCAAGGAACAATTAGTTATCATGCCTACAGCTCGCGAACCTGGACCGGTGCCACCAGCACCTCCAGTAGCTCAACCTACCAGTCCAGTTTCAGCATTAACGGACCCTGGGGCTATGCTGGCCGGCTTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1020

Amino Acids

113.61

Weight (kDa)

5.73

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 1.7e-114 Adaptin N terminal region
Cnd1 PF12717 125 - 265 4.9e-07 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 757 - 853 2.3e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 865 - 974 6.7e-16 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 338
Acc36I ACCTGC 2 cut(s) 253, 1450
Acc65I GGTACC 1 cut(s) 1585
AccB1I GGYRCC 3 cut(s) 1585, 2050, 2974
AccB7I CCANNNNNTGG 2 cut(s) 376, 1504
AccBSI CCGCTC 1 cut(s) 1795
AccI GTMKAC 1 cut(s) 1126
AccII CGCG 1 cut(s) 2961
AclI AACGTT 1 cut(s) 2795
AclWI GGATC 8 cut(s) 797, 1106, 1119, 1992, 2036, 2153, 2282, 2843
AcoI YGGCCR 4 cut(s) 406, 1495, 1506, 3045
AcsI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
AcuI CTGAAG 8 cut(s) 740, 764, 2040, 2163, 2582, 2640, 2684, 2871
AfiI CCNNNNNNNGG 6 cut(s) 376, 1504, 1740, 1922, 2150, 2322
AflII CTTAAG 2 cut(s) 1250, 1735
AgeI ACCGGT 1 cut(s) 2971
AhdI GACNNNNNGTC 1 cut(s) 2005
AhlI ACTAGT 1 cut(s) 2434
AjnI CCWGG 7 cut(s) 705, 749, 1516, 2143, 2456, 2965, 3031
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
AloI GAACNNNNNNTCC 4 cut(s) 993, 1025, 2328, 2360
Alw21I GWGCWC 2 cut(s) 1141, 2430
Alw26I GTCTC 7 cut(s) 356, 963, 1072, 1251, 1329, 1448, 2016
AlwI GGATC 8 cut(s) 797, 1106, 1119, 1992, 2036, 2153, 2282, 2843
AlwNI CAGNNNCTG 1 cut(s) 2615
Ama87I CYCGRG 1 cut(s) 2909
ApaI GGGCCC 1 cut(s) 1873
ApeKI GCWGC 8 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1751, 1876
ApoI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
AseI ATTAAT 1 cut(s) 1319
AsiGI ACCGGT 1 cut(s) 2971
Asp700I GAANNNNTTC 2 cut(s) 2244, 2921
Asp718I GGTACC 1 cut(s) 1585
AspLEI GCGC 3 cut(s) 244, 339, 510
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 5 cut(s) 1063, 1090, 2084, 2168, 2364
AvaI CYCGRG 1 cut(s) 2909
AvaII GGWCC 5 cut(s) 1926, 2084, 2893, 2969, 3028
AxyI CCTNAGG 1 cut(s) 2046
BaeGI GKGCMC 3 cut(s) 216, 1873, 2464
BaeI ACNNNNGTAYC 4 cut(s) 846, 879, 1818, 1851
BalI TGGCCA 2 cut(s) 1497, 1508
BamHI GGATCC 1 cut(s) 1111
BanI GGYRCC 3 cut(s) 1585, 2050, 2974
BanII GRGCYC 3 cut(s) 778, 1873, 2430
BarI GAAGNNNNNNTAC 2 cut(s) 1578, 1610
BbsI GAAGAC 1 cut(s) 1398
Bbv12I GWGCWC 2 cut(s) 1141, 2430
BbvCI CCTCAGC 1 cut(s) 2127
BbvI GCAGC 8 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1763, 1888
BccI CCATC 5 cut(s) 539, 556, 751, 844, 2737
BceAI ACGGC 2 cut(s) 1529, 2153
BciT130I CCWGG 7 cut(s) 707, 751, 1518, 2145, 2458, 2967, 3033
BciVI GTATCC 2 cut(s) 261, 2437
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 7 cut(s) 356, 963, 1072, 1251, 1329, 1448, 2016
BcuI ACTAGT 1 cut(s) 2434
BfaI CTAG 7 cut(s) 459, 699, 1142, 1422, 1433, 2435, 2502
BfmI CTRYAG 3 cut(s) 466, 1649, 2952
BfrI CTTAAG 2 cut(s) 1250, 1735
BfuAI ACCTGC 2 cut(s) 253, 1450
BfuI GTATCC 2 cut(s) 261, 2437
BglII AGATCT 2 cut(s) 1614, 1659
Bme1390I CCNGG 8 cut(s) 83, 707, 751, 1518, 2145, 2458, 2967, 3033
Bme18I GGWCC 5 cut(s) 1926, 2084, 2893, 2969, 3028
BmeRI GACNNNNNGTC 1 cut(s) 2005
BmeT110I CYCGRG 1 cut(s) 2909
BmrFI CCNGG 8 cut(s) 83, 707, 751, 1518, 2145, 2458, 2967, 3033
BmsI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1950, 2155, 2164
BpiI GAAGAC 1 cut(s) 1398
BpmI CTGGAG 4 cut(s) 676, 1353, 1620, 2973
Bpu10I CCTNAGC 2 cut(s) 1791, 2127
BpuMI CCSGG 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 1130
BsaWI WCCGGW 3 cut(s) 558, 2440, 2971
BsaXI ACNNNNNCTCC 8 cut(s) 941, 971, 1326, 1356, 1618, 1648, 2437, 2467
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1504, 1740, 1922, 2150, 2322
Bse118I RCCGGY 2 cut(s) 2971, 3047
Bse1I ACTGG 6 cut(s) 659, 1942, 2609, 2990, 3006, 3011
Bse21I CCTNAGG 1 cut(s) 2046
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 7 cut(s) 707, 751, 1518, 2145, 2458, 2967, 3033
BseGI GGATG 5 cut(s) 567, 738, 2179, 2342, 2515
BseLI CCNNNNNNNGG 6 cut(s) 376, 1504, 1740, 1922, 2150, 2322
BseMI GCAATG 1 cut(s) 346
BseNI ACTGG 6 cut(s) 659, 1942, 2609, 2990, 3006, 3011
BseRI GAGGAG 1 cut(s) 2216
BseSI GKGCMC 3 cut(s) 216, 1873, 2464
BseXI GCAGC 8 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1763, 1888
BseYI CCCAGC 6 cut(s) 97, 190, 549, 1872, 1964, 2575
BsgI GTGCAG 3 cut(s) 477, 1543, 1770
Bsh1236I CGCG 1 cut(s) 2961
BshNI GGYRCC 3 cut(s) 1585, 2050, 2974
BshTI ACCGGT 1 cut(s) 2971
BsiHKAI GWGCWC 2 cut(s) 1141, 2430
BsiHKCI CYCGRG 1 cut(s) 2909
BsiSI CCGG 6 cut(s) 82, 559, 2441, 2753, 2972, 3048
BslFI GGGAC 3 cut(s) 1939, 2070, 2906
BslI CCNNNNNNNGG 6 cut(s) 376, 1504, 1740, 1922, 2150, 2322
BsmAI GTCTC 7 cut(s) 356, 963, 1072, 1251, 1329, 1448, 2016
BsmBI CGTCTC 1 cut(s) 2016
BsmFI GGGAC 3 cut(s) 1939, 2070, 2906
BsoBI CYCGRG 1 cut(s) 2909
Bsp120I GGGCCC 1 cut(s) 1869
Bsp1286I GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
Bsp19I CCATGG 1 cut(s) 2088
Bsp68I TCGCGA 1 cut(s) 2961
BspFNI CGCG 1 cut(s) 2961
BspMI ACCTGC 2 cut(s) 253, 1450
BspPI GGATC 8 cut(s) 797, 1106, 1119, 1992, 2036, 2153, 2282, 2843
BspQI GCTCTTC 1 cut(s) 783
BspT107I GGYRCC 3 cut(s) 1585, 2050, 2974
BspTI CTTAAG 2 cut(s) 1250, 1735
BsrBI CCGCTC 1 cut(s) 1795
BsrDI GCAATG 1 cut(s) 346
BsrFI RCCGGY 2 cut(s) 2971, 3047
BsrI ACTGG 6 cut(s) 659, 1942, 2609, 2990, 3006, 3011
BssAI RCCGGY 2 cut(s) 2971, 3047
BssNAI GTATAC 1 cut(s) 1127
BssT1I CCWWGG 3 cut(s) 2002, 2068, 2088
Bst1107I GTATAC 1 cut(s) 1127
Bst2UI CCWGG 7 cut(s) 707, 751, 1518, 2145, 2458, 2967, 3033
Bst4CI ACNGT 6 cut(s) 334, 796, 1585, 2732, 2868, 2881
Bst6I CTCTTC 2 cut(s) 783, 2616
BstAFI CTTAAG 2 cut(s) 1250, 1735
BstAPI GCANNNNNTGC 2 cut(s) 896, 1226
BstBAI YACGTR 1 cut(s) 1130
BstDSI CCRYGG 2 cut(s) 2088, 2169
BstENI CCTNNNNNAGG 2 cut(s) 1738, 2148
BstF5I GGATG 5 cut(s) 567, 738, 2179, 2342, 2515
BstFNI CGCG 1 cut(s) 2961
BstHHI GCGC 3 cut(s) 244, 339, 510
BstMAI GTCTC 7 cut(s) 356, 963, 1072, 1251, 1329, 1448, 2016
BstNI CCWGG 7 cut(s) 707, 751, 1518, 2145, 2458, 2967, 3033
BstNSI RCATGY 4 cut(s) 19, 288, 899, 1045
BstSCI CCNGG 8 cut(s) 81, 705, 749, 1516, 2143, 2456, 2965, 3031
BstSFI CTRYAG 3 cut(s) 466, 1649, 2952
BstSLI GKGCMC 3 cut(s) 216, 1873, 2464
BstSNI TACGTA 1 cut(s) 1130
BstUI CGCG 1 cut(s) 2961
BstV1I GCAGC 8 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1763, 1888
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 6 cut(s) 802, 1111, 1614, 1659, 2287, 2848
BstXI CCANNNNNNTGG 1 cut(s) 750
BstYI RGATCY 6 cut(s) 802, 1111, 1614, 1659, 2287, 2848
BstZ17I GTATAC 1 cut(s) 1127
Bsu36I CCTNAGG 1 cut(s) 2046
BsuI GTATCC 2 cut(s) 261, 2437
BtgI CCRYGG 2 cut(s) 2088, 2169
BtsCI GGATG 5 cut(s) 567, 738, 2179, 2342, 2515
BtsIMutI CAGTG 4 cut(s) 968, 1477, 2616, 2642
BtuMI TCGCGA 1 cut(s) 2961
BveI ACCTGC 2 cut(s) 253, 1450
CaiI CAGNNNCTG 1 cut(s) 2615
CfoI GCGC 3 cut(s) 244, 339, 510
Cfr10I RCCGGY 2 cut(s) 2971, 3047
CseI GACGC 1 cut(s) 1534
CspAI ACCGGT 1 cut(s) 2971
DriI GACNNNNNGTC 1 cut(s) 2005
EaeI YGGCCR 4 cut(s) 406, 1495, 1506, 3045
Eam1104I CTCTTC 2 cut(s) 783, 2616
Eam1105I GACNNNNNGTC 1 cut(s) 2005
EarI CTCTTC 2 cut(s) 783, 2616
Ecl136II GAGCTC 1 cut(s) 2428
Eco105I TACGTA 1 cut(s) 1130
Eco130I CCWWGG 3 cut(s) 2002, 2068, 2088
Eco147I AGGCCT 1 cut(s) 24
Eco24I GRGCYC 3 cut(s) 778, 1873, 2430
Eco32I GATATC 4 cut(s) 1016, 1069, 1120, 2065
Eco47I GGWCC 5 cut(s) 1926, 2084, 2893, 2969, 3028
Eco53kI GAGCTC 1 cut(s) 2428
Eco57I CTGAAG 8 cut(s) 740, 764, 2040, 2163, 2582, 2640, 2684, 2871
Eco81I CCTNAGG 1 cut(s) 2046
Eco88I CYCGRG 1 cut(s) 2909
EcoICRI GAGCTC 1 cut(s) 2428
EcoNI CCTNNNNNAGG 2 cut(s) 1738, 2148
EcoO109I RGGNCCY 4 cut(s) 1926, 2084, 2102, 2893
EcoRII CCWGG 7 cut(s) 705, 749, 1516, 2143, 2456, 2965, 3031
EcoRV GATATC 4 cut(s) 1016, 1069, 1120, 2065
EcoT14I CCWWGG 3 cut(s) 2002, 2068, 2088
EcoT22I ATGCAT 2 cut(s) 892, 933
EcoT38I GRGCYC 3 cut(s) 778, 1873, 2430
ErhI CCWWGG 3 cut(s) 2002, 2068, 2088
Esp3I CGTCTC 1 cut(s) 2016
FalI AAGNNNNNCTT 2 cut(s) 2325, 2357
FaqI GGGAC 3 cut(s) 1939, 2070, 2906
FauI CCCGC 5 cut(s) 1046, 1860, 1938, 2116, 2484
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 1126
FokI GGATG 5 cut(s) 574, 725, 2186, 2349, 2522
FriOI GRGCYC 3 cut(s) 778, 1873, 2430
FspBI CTAG 7 cut(s) 459, 699, 1142, 1422, 1433, 2435, 2502
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 3 cut(s) 243, 338, 509
GsaI CCCAGC 6 cut(s) 101, 194, 553, 1876, 1968, 2579
GsuI CTGGAG 4 cut(s) 676, 1353, 1620, 2973
HapII CCGG 6 cut(s) 82, 559, 2441, 2753, 2972, 3048
HgaI GACGC 1 cut(s) 1534
HhaI GCGC 3 cut(s) 244, 339, 510
Hin6I GCGC 3 cut(s) 242, 337, 508
HinP1I GCGC 3 cut(s) 242, 337, 508
HincII GTYRAC 3 cut(s) 384, 600, 1939
HindII GTYRAC 3 cut(s) 384, 600, 1939
HindIII AAGCTT 3 cut(s) 1559, 2564, 2666
HinfI GANTC 5 cut(s) 422, 1309, 1654, 2491, 2719
HpaI GTTAAC 1 cut(s) 600
HpaII CCGG 6 cut(s) 82, 559, 2441, 2753, 2972, 3048
HphI GGTGA 5 cut(s) 1063, 1090, 2084, 2168, 2364
Hpy166II GTNNAC 7 cut(s) 113, 384, 600, 726, 1127, 1939, 2813
Hpy8I GTNNAC 7 cut(s) 113, 384, 600, 726, 1127, 1939, 2813
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 7 cut(s) 626, 983, 1102, 1810, 2064, 2093, 2911
HpyCH4III ACNGT 6 cut(s) 334, 796, 1585, 2732, 2868, 2881
HpyCH4IV ACGT 3 cut(s) 583, 1129, 2795
HpySE526I ACGT 3 cut(s) 583, 1129, 2795
HspAI GCGC 3 cut(s) 242, 337, 508
KflI GGGWCCC 3 cut(s) 1926, 2084, 2893
KpnI GGTACC 1 cut(s) 1589
KroI GCCGGC 1 cut(s) 3047
KroNI GCCGGC 1 cut(s) 3049
Ksp22I TGATCA 1 cut(s) 1809
KspAI GTTAAC 1 cut(s) 600
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 4 cut(s) 84, 94, 2203, 2425
Lsp1109I GCAGC 8 cut(s) 474, 478, 1243, 1421, 1424, 1705, 1763, 1888
LweI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1950, 2155, 2164
MaeI CTAG 7 cut(s) 459, 699, 1142, 1422, 1433, 2435, 2502
MaeII ACGT 3 cut(s) 583, 1129, 2795
MaeIII GTNAC 6 cut(s) 277, 1051, 1378, 1570, 1900, 2543
MbiI CCGCTC 1 cut(s) 1795
MfeI CAATTG 1 cut(s) 2184
MflI RGATCY 6 cut(s) 802, 1111, 1614, 1659, 2287, 2848
MhlI GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
MlsI TGGCCA 2 cut(s) 1497, 1508
MluNI TGGCCA 2 cut(s) 1497, 1508
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 335, 815
Mox20I TGGCCA 2 cut(s) 1497, 1508
Mph1103I ATGCAT 2 cut(s) 892, 933
MroNI GCCGGC 1 cut(s) 3047
MroXI GAANNNNTTC 2 cut(s) 2244, 2921
MscI TGGCCA 2 cut(s) 1497, 1508
MslI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
Msp20I TGGCCA 2 cut(s) 1497, 1508
MspA1I CMGCKG 3 cut(s) 841, 1844, 2579
MspCI CTTAAG 2 cut(s) 1250, 1735
MspI CCGG 6 cut(s) 82, 559, 2441, 2753, 2972, 3048
MspR9I CCNGG 8 cut(s) 83, 707, 751, 1518, 2145, 2458, 2967, 3033
MunI CAATTG 1 cut(s) 2184
MvaI CCWGG 7 cut(s) 707, 751, 1518, 2145, 2458, 2967, 3033
MvnI CGCG 1 cut(s) 2961
NaeI GCCGGC 1 cut(s) 3049
NciI CCSGG 1 cut(s) 83
NcoI CCATGG 1 cut(s) 2088
NdeI CATATG 1 cut(s) 1606
NgoMIV GCCGGC 1 cut(s) 3047
NmeAIII GCCGAG 2 cut(s) 434, 2341
NmuCI GTSAC 3 cut(s) 1051, 1570, 1900
NruI TCGCGA 1 cut(s) 2961
NsbI TGCGCA 1 cut(s) 338
NsiI ATGCAT 2 cut(s) 892, 933
NspI RCATGY 4 cut(s) 19, 288, 899, 1045
PaeI GCATGC 2 cut(s) 19, 288
PaeR7I CTCGAG 1 cut(s) 2909
PasI CCCWGGG 2 cut(s) 2457, 3032
PceI AGGCCT 1 cut(s) 24
PciSI GCTCTTC 1 cut(s) 783
PdiI GCCGGC 1 cut(s) 3049
PdmI GAANNNNTTC 2 cut(s) 2244, 2921
PfeI GAWTC 4 cut(s) 1309, 1654, 2491, 2719
PflMI CCANNNNNTGG 2 cut(s) 376, 1504
PinAI ACCGGT 1 cut(s) 2971
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 1130
PpuMI RGGWCCY 3 cut(s) 1926, 2084, 2893
PshBI ATTAAT 1 cut(s) 1319
Psp124BI GAGCTC 1 cut(s) 2430
Psp1406I AACGTT 1 cut(s) 2795
Psp5II RGGWCCY 3 cut(s) 1926, 2084, 2893
Psp6I CCWGG 7 cut(s) 705, 749, 1516, 2143, 2456, 2965, 3031
PspFI CCCAGC 6 cut(s) 97, 190, 549, 1872, 1964, 2575
PspGI CCWGG 7 cut(s) 705, 749, 1516, 2143, 2456, 2965, 3031
PspOMI GGGCCC 1 cut(s) 1869
PspPPI RGGWCCY 3 cut(s) 1926, 2084, 2893
PspXI VCTCGAGB 1 cut(s) 2909
PstNI CAGNNNCTG 1 cut(s) 2615
PsuI RGATCY 6 cut(s) 802, 1111, 1614, 1659, 2287, 2848
PvuII CAGCTG 1 cut(s) 2579
RruI TCGCGA 1 cut(s) 2961
RseI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SacI GAGCTC 1 cut(s) 2430
SapI GCTCTTC 1 cut(s) 783
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 8 cut(s) 83, 707, 751, 1518, 2145, 2458, 2967, 3033
SduI GDGCHC 6 cut(s) 216, 778, 1141, 1873, 2430, 2464
SfaNI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1950, 2155, 2164
SfcI CTRYAG 3 cut(s) 466, 1649, 2952
Sfr274I CTCGAG 1 cut(s) 2909
SinI GGWCC 5 cut(s) 1926, 2084, 2893, 2969, 3028
SlaI CTCGAG 1 cut(s) 2909
SmiMI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SmlI CTYRAG 3 cut(s) 1250, 1735, 2909
SmoI CTYRAG 3 cut(s) 1250, 1735, 2909
SnaBI TACGTA 1 cut(s) 1130
SpeI ACTAGT 1 cut(s) 2434
SphI GCATGC 2 cut(s) 19, 288
SseBI AGGCCT 1 cut(s) 24
SspI AATATT 5 cut(s) 400, 684, 2233, 2296, 2400
SspMI CTAG 7 cut(s) 459, 699, 1142, 1422, 1433, 2435, 2502
SstI GAGCTC 1 cut(s) 2430
StuI AGGCCT 1 cut(s) 24
StyD4I CCNGG 8 cut(s) 81, 705, 749, 1516, 2143, 2456, 2965, 3031
StyI CCWWGG 3 cut(s) 2002, 2068, 2088
TaaI ACNGT 6 cut(s) 334, 796, 1585, 2732, 2868, 2881
TaiI ACGT 3 cut(s) 586, 1132, 2798
TaqI TCGA 5 cut(s) 87, 798, 1191, 1886, 2910
TaqII GACCGA 1 cut(s) 1919
TatI WGTACW 3 cut(s) 723, 2789, 2810
TauI GCSGC 2 cut(s) 1798, 2326
TfiI GAWTC 4 cut(s) 1309, 1654, 2491, 2719
TscAI CASTG 4 cut(s) 968, 1477, 2616, 2642
TseFI GTSAC 3 cut(s) 1051, 1570, 1900
TseI GCWGC 8 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1751, 1876
Tsp45I GTSAC 3 cut(s) 1051, 1570, 1900
TspDTI ATGAA 5 cut(s) 25, 162, 594, 2237, 2914
TspGWI ACGGA 2 cut(s) 98, 3041
TspRI CASTG 4 cut(s) 968, 1477, 2616, 2642
Van91I CCANNNNNTGG 2 cut(s) 376, 1504
Vha464I CTTAAG 2 cut(s) 1250, 1735
VpaK11BI GGWCC 5 cut(s) 1926, 2084, 2893, 2969, 3028
VspI ATTAAT 1 cut(s) 1319
XagI CCTNNNNNAGG 2 cut(s) 1738, 2148
XapI RAATTY 6 cut(s) 413, 983, 1490, 1784, 2209, 2591
XbaI TCTAGA 2 cut(s) 1141, 1432
XceI RCATGY 4 cut(s) 19, 288, 899, 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 2827
XhoI CTCGAG 1 cut(s) 2909
XmiI GTMKAC 1 cut(s) 1126
XmnI GAANNNNTTC 2 cut(s) 2244, 2921
XspI CTAG 7 cut(s) 459, 699, 1142, 1422, 1433, 2435, 2502
Zsp2I ATGCAT 2 cut(s) 892, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.