Rw5G034280

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007314
Physical Location & Seq
Reverse (-)
59008074 .. 59020282
12209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034280.1

Sequence Viewer

Length: 3051 bp
ATGCGAGGTCTCTCTGTTTTCATCAGCGACATTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTCCGTGTCGATAAGGAGCTCGGGAATGTTCGTACGCGCTTCAAAAACGAAAAGGGTTTGACACCGTATGAGAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGTTGGGATATGATGTGGATTTTGGTCACATGGAAGCAGTCTCTCTTATATCAGCGCCTAAGTATCCAGAAAAGCAGGTTGGGTACATTGTGACATCATGTCTACTCAATGAAAACCACGATTTTCTGAGATTAGCGATCAATACTGTACGGAATGATATTATTGGTCGTAATGAAACTTTCCAATGTTTGGCATTGACTATGGTTGGGAATATTGGAGGCAGAGAATTTGCAGAGTCATTAGCACCTGATGTTCAAAAGTTGCTTATTTCTAGCAGCTACAGACCGCTTGTGAGAAAAAAAGCTGCACTATGTCTTTTGCGCTTGTATAGAAAAAATCCTGATGTTGTCAATATAGATGGCTGGGCCGATCGCATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTGACATCATCTATGAGCCTTCTTGTTGCGCTTGTATCAAATCACCACGATGCTTACTGGAGTTGTCTTCCAAAATGTGTTAAAATACTAGAACGGCTTGCGAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGCATCCCATCTCCCTGGCTCCAGGTCAAGACAATGAGGGTTCTGCAGTATTTCCCCACTGTTGAAGATCCAAATACAAGAAGATCATTGTTTGAGGTCTTACAACGTATATTGATGGGTACGGATGTTGTGAAAAATGTTAATAAGAACAACGCATCACACGCTGTTCTTTTTGAAGCCCTCGCACTTGTCATGCATCTTGATGCTGAGAAGGAGATGATGTCCCAATGTGTTGCACTTCTGGGAAAATTTATTGCTGTGCGAGAACCAAATATTCGATATCTTGGTTTGGAAAATATGACCCGCATGCTAATGGTAACGGATGTGCAAGATATCATCAAGAGACATCAAGCCCAGATTATCACCTCATTGAAGGATCCAGATGTCAGCATTCGTAGGCGTGCTCTTGACCTGCTTTATGGCATGTGTGATGTTTCAAATGCAAAAGACATAGTTGAAGAATTACTGCAGTATCTTAGTACAGCAGACTTCGCAATGCGTGAGGAATTGTCACTTAAGGCTGCCATTCTTGCAGAAAAATTTGCTCCTGATCTATCATGGTATGTAGATGTGATTCTTCAATTGATTGATAAGGCAGGAGATTTTGTTAGTGATGATATATGGTTCCGTGTTGTGCAATTTGTTACGAATAATGAAGACTTACAGCCTTATGCTGCAGCAAAAGCCAGAGAGTATCTTGACAAACCTGCTATACATGAGACAATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGTCACCTTTTAGCCAGACGGCCTGGGTGTAGTCCAAAAGAATTATTTGCCGTCATACATGAGAAGCTTCCTACTGTATCCACTTCTACAATACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAAGAACTACAGAATCAGATTTGGGCAATATTCAATAAATACGAGAGTTGCATCGATGTTGAGATTCAACAACGAGCTGCTGAATACCTTGCCTTAAGTAGGAGGGGTGAAGCTCTTGTTGATATATTGGCTGAAATGCCTAAATTCCCCGAGCGGCAGTCTGCATTGATCAAAAAGGCTGAAGATACTGAAGTTGATACCGCAGAACAAAGTGCTATCAAGTTGCGTGCCCAGCAGCAAACATCAAATGCTTTGGTTGTGACCGACCAACGTCCCGCTAATGGTACCCCACCTGCCAACCACCAGCTTGGTCTTGTGAAGATACCCAACATGAGCAGTAATGCGGATCACAATTCAACCGATCAAGGGTTGTCTCAGGAAAATGGTACTTTGAATAAAGTTGATCCTCAAACTCCTTCACCGGATCTCCTTGGTGACCTCTTGGGTCCACTGGCAATTGAAGGCCCTCCGGGTACTGCCGTTCAATCACATCAAACTGAAATCCCTGGATCTGGTGGTGACCCAAATGCAGTGGATGCTACGGCCATAGTTCCTGTTGGCGAAGAGCCAAATTCTGTTCAGCCTATAGGAAATATTGCAGAAAGATTTCATGCCTTGTGCTTGAAAGATAGTGGCGTTCTGTATGAAGATCCTAATATTCAGATCGGAATTAAAGCAGAGTGGCGCTTACATCAGGGATGTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGTATCAGTTCAGGCTGTAATAATGCCTCCCTCCCACTTTAAAATGGAGCTCTCACTAGTGCCAGAGACTATTCCTCCTAGGGCACAGGTACAGTGCCCCCTTGAAGTTGTGAATCTCCGCCCAAGTAGGGATGTAGCAGTTCTTGACTTCTCTTACAAGTTCGGTCACAATATGGTCAATGTCAAGCTTCGTCTCCCAGCTGTCTTGAATAAATTTCTTCAACCTATACCAGTATCTGCTGAAGAGTTCTTTCCTCAGTGGAGATCACTCACAGGACCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGCGTCAAACCACTGCCACTTGCAGAAATGGCAAATCTAATTAACAGCTTCCGGTTTATGGTTTGTCCAGGGCTTGACCCTAATCCAAATAATCTTGTTGCAAGCACAACCTTTTACTCAGAAAGTACACGGGCGATGCTTTGCTTGGCTAGAATAGAAACAGATCCAGCAGACAGAACCCAGTTGCGTATGACGGTTGCTTCAGGGGATCCTACGTTAACATTTGAGTTGAAGGAGTTCATCAAGGAACAAATAGTTAACATCCCTGTAGCTCCTCGTGCACCTGGACCAGTGCCACCAGCACCTCCAGTAGCTCAACCGACCAGTCCAGCTGCAGCATTAACGGATCCTGGTGCTCTGCTGGCTGGTCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1016

Amino Acids

113.35

Weight (kDa)

5.94

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 23 - 577 1.9e-116 Adaptin N terminal region
Cnd1 PF12717 120 - 258 1.1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 753 - 849 1.9e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 861 - 970 2.4e-15 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1927
Acc36I ACCTGC 4 cut(s) 238, 1140, 1435, 1927
Acc65I GGTACC 1 cut(s) 1910
AccB1I GGYRCC 1 cut(s) 1910
AccB7I CCANNNNNTGG 1 cut(s) 361
AccBSI CCGCTC 1 cut(s) 1780
AccI GTMKAC 1 cut(s) 274
AccII CGCG 1 cut(s) 100
AciI CCGC 7 cut(s) 458, 1024, 1780, 1827, 1902, 1970, 2485
AcoI YGGCCR 1 cut(s) 2169
AcsI RAATTY 7 cut(s) 398, 968, 1259, 1475, 1769, 2197, 2579
AcuI CTGAAG 5 cut(s) 1827, 1836, 2628, 2672, 2859
AcyI GRCGYC 1 cut(s) 2676
AfiI CCNNNNNNNGG 7 cut(s) 361, 1725, 1907, 1992, 2138, 2494, 2731
AflII CTTAAG 2 cut(s) 1235, 1720
AhdI GACNNNNNGTC 1 cut(s) 270
AhlI ACTAGT 1 cut(s) 2422
AjnI CCWGG 8 cut(s) 690, 734, 741, 1501, 2131, 2740, 2956, 3022
AjuI GAANNNNNNNTTGG 4 cut(s) 234, 266, 558, 590
AloI GAACNNNNNNTCC 2 cut(s) 2316, 2348
Alw21I GWGCWC 5 cut(s) 84, 1126, 2418, 2956, 3031
Alw26I GTCTC 7 cut(s) 14, 217, 1057, 1433, 2004, 2426, 2564
Alw44I GTGCAC 1 cut(s) 2952
AlwNI CAGNNNCTG 1 cut(s) 2603
Ama87I CYCGRG 2 cut(s) 83, 1775
AoxI GGCC 4 cut(s) 537, 1499, 2089, 2169
ApaLI GTGCAC 1 cut(s) 2952
ApeKI GCWGC 9 cut(s) 447, 476, 1241, 1394, 1397, 1703, 1861, 3005, 3008
ApoI RAATTY 7 cut(s) 398, 968, 1259, 1475, 1769, 2197, 2579
Asp700I GAANNNNTTC 2 cut(s) 2232, 2909
Asp718I GGTACC 1 cut(s) 1910
AspA2I CCTAGG 1 cut(s) 2444
AspLEI GCGC 5 cut(s) 102, 229, 495, 613, 2313
AspS9I GGNCC 5 cut(s) 537, 2072, 2090, 2642, 2960
AsuC2I CCSGG 1 cut(s) 2097
AsuHPI GGTGA 8 cut(s) 617, 1075, 1475, 1745, 2037, 2072, 2156, 2352
AvaI CYCGRG 2 cut(s) 83, 1775
AvaII GGWCC 3 cut(s) 2072, 2642, 2960
AvrII CCTAGG 1 cut(s) 2444
BaeGI GKGCMC 4 cut(s) 1858, 2452, 2465, 2956
BaeI ACNNNNGTAYC 8 cut(s) 831, 864, 1175, 1208, 1803, 1836, 2091, 2124
BamHI GGATCC 3 cut(s) 1096, 2881, 3019
BanI GGYRCC 1 cut(s) 1910
BanII GRGCYC 2 cut(s) 84, 2418
BauI CACGAG 1 cut(s) 2949
BbsI GAAGAC 2 cut(s) 641, 1383
Bbv12I GWGCWC 5 cut(s) 84, 1126, 2418, 2956, 3031
BbvI GCAGC 9 cut(s) 459, 463, 1228, 1381, 1409, 1690, 1873, 2992, 3020
BccI CCATC 3 cut(s) 524, 736, 829
BceAI ACGGC 5 cut(s) 692, 1514, 1514, 2090, 2184
BciT130I CCWGG 8 cut(s) 692, 736, 743, 1503, 2133, 2742, 2958, 3024
BciVI GTATCC 2 cut(s) 246, 1567
BclI TGATCA 1 cut(s) 1794
BcnI CCSGG 1 cut(s) 2097
BcoDI GTCTC 7 cut(s) 14, 217, 1057, 1433, 2004, 2426, 2564
BcuI ACTAGT 1 cut(s) 2422
BfaI CTAG 5 cut(s) 444, 671, 2423, 2445, 2823
BfmI CTRYAG 8 cut(s) 451, 764, 1187, 1395, 1634, 2211, 2940, 3006
BfoI RGCGCY 2 cut(s) 230, 2314
BfrI CTTAAG 2 cut(s) 1235, 1720
BfuAI ACCTGC 4 cut(s) 238, 1140, 1435, 1927
BfuI GTATCC 2 cut(s) 246, 1567
BglII AGATCT 1 cut(s) 1599
BlnI CCTAGG 1 cut(s) 2444
Bme1390I CCNGG 9 cut(s) 692, 736, 743, 1503, 2097, 2133, 2742, 2958, 3024
Bme18I GGWCC 3 cut(s) 2072, 2642, 2960
BmeRI GACNNNNNGTC 1 cut(s) 270
BmeT110I CYCGRG 2 cut(s) 83, 1775
BmgT120I GGNCC 5 cut(s) 537, 2072, 2090, 2642, 2960
BmiI GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1912, 2073, 2883, 3021
BmrFI CCNGG 9 cut(s) 692, 736, 743, 1503, 2097, 2133, 2742, 2958, 3024
BmrI ACTGGG 1 cut(s) 2848
BmsI GCATC 8 cut(s) 622, 732, 884, 913, 925, 1686, 2152, 2799
BmuI ACTGGG 1 cut(s) 2848
BoxI GACNNNNGTC 1 cut(s) 1896
BpiI GAAGAC 2 cut(s) 641, 1383
BpmI CTGGAG 4 cut(s) 661, 725, 1605, 2964
BpuMI CCSGG 1 cut(s) 2097
Bsa29I ATCGAT 1 cut(s) 1680
BsaHI GRCGYC 1 cut(s) 2676
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 6 cut(s) 734, 1502, 2056, 2131, 2444, 2741
BsaWI WCCGGW 2 cut(s) 2047, 2724
Bsc4I CCNNNNNNNGG 7 cut(s) 361, 1725, 1907, 1992, 2138, 2494, 2731
Bse1I ACTGG 7 cut(s) 644, 2082, 2597, 2854, 2963, 2981, 2997
Bse3DI GCAATG 1 cut(s) 1221
BseBI CCWGG 8 cut(s) 692, 736, 743, 1503, 2133, 2742, 2958, 3024
BseCI ATCGAT 1 cut(s) 1680
BseDI CCNNGG 6 cut(s) 734, 1502, 2056, 2131, 2444, 2741
BseGI GGATG 7 cut(s) 723, 850, 1048, 2167, 2330, 2503, 2934
BseLI CCNNNNNNNGG 7 cut(s) 361, 1725, 1907, 1992, 2138, 2494, 2731
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 5 cut(s) 290, 918, 2015, 2636, 2805
BseNI ACTGG 7 cut(s) 644, 2082, 2597, 2854, 2963, 2981, 2997
BseRI GAGGAG 1 cut(s) 2937
BseSI GKGCMC 4 cut(s) 1858, 2452, 2465, 2956
BseXI GCAGC 9 cut(s) 459, 463, 1228, 1381, 1409, 1690, 1873, 2992, 3020
BseYI CCCAGC 3 cut(s) 534, 1857, 2563
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 544
BshFI GGCC 4 cut(s) 539, 1501, 2091, 2171
BshNI GGYRCC 1 cut(s) 1910
BshVI ATCGAT 1 cut(s) 1680
BsiEI CGRYCG 1 cut(s) 544
BsiHKAI GWGCWC 5 cut(s) 84, 1126, 2418, 2956, 3031
BsiHKCI CYCGRG 2 cut(s) 83, 1775
BsiSI CCGG 3 cut(s) 2048, 2096, 2725
BsiWI CGTACG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 928, 1884
BslI CCNNNNNNNGG 7 cut(s) 361, 1725, 1907, 1992, 2138, 2494, 2731
BsmAI GTCTC 7 cut(s) 14, 217, 1057, 1433, 2004, 2426, 2564
BsmBI CGTCTC 1 cut(s) 2564
BsmFI GGGAC 2 cut(s) 928, 1884
BsmI GAATGC 1 cut(s) 1110
BsnI GGCC 4 cut(s) 539, 1501, 2091, 2171
Bso31I GGTCTC 1 cut(s) 14
BsoBI CYCGRG 2 cut(s) 83, 1775
Bsp1286I GDGCHC 8 cut(s) 84, 1126, 1858, 2418, 2452, 2465, 2956, 3031
BspACI CCGC 7 cut(s) 458, 1024, 1780, 1827, 1902, 1970, 2485
BspANI GGCC 4 cut(s) 539, 1501, 2091, 2171
BspCNI CTCAG 5 cut(s) 291, 919, 2014, 2635, 2804
BspDI ATCGAT 1 cut(s) 1680
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1912, 2073, 2883, 3021
BspMAI CTGCAG 4 cut(s) 768, 1191, 1399, 3010
BspMI ACCTGC 4 cut(s) 238, 1140, 1435, 1927
BspQI GCTCTTC 1 cut(s) 2184
BspT107I GGYRCC 1 cut(s) 1910
BspTI CTTAAG 2 cut(s) 1235, 1720
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 1780
BsrDI GCAATG 1 cut(s) 1221
BsrI ACTGG 7 cut(s) 644, 2082, 2597, 2854, 2963, 2981, 2997
BssECI CCNNGG 6 cut(s) 734, 1502, 2056, 2131, 2444, 2741
BssNI GRCGYC 1 cut(s) 2676
BssSI CACGAG 1 cut(s) 2949
BssT1I CCWWGG 2 cut(s) 2056, 2444
Bst2BI CACGAG 1 cut(s) 2949
Bst2UI CCWGG 8 cut(s) 692, 736, 743, 1503, 2133, 2742, 2958, 3024
Bst4CI ACNGT 6 cut(s) 129, 319, 781, 1555, 2460, 2869
Bst6I CTCTTC 2 cut(s) 2184, 2604
BstACI GRCGYC 1 cut(s) 2676
BstAFI CTTAAG 2 cut(s) 1235, 1720
BstAPI GCANNNNNTGC 1 cut(s) 2162
BstC8I GCNNGC 6 cut(s) 681, 1028, 1122, 1854, 2776, 3036
BstDEI CTNAG 8 cut(s) 231, 299, 927, 1196, 1596, 2001, 2622, 2791
BstEII GGTNACC 3 cut(s) 1481, 2060, 2144
BstENI CCTNNNNNAGG 1 cut(s) 1723
BstF5I GGATG 7 cut(s) 723, 850, 1048, 2167, 2330, 2503, 2934
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 230, 2314
BstHHI GCGC 5 cut(s) 102, 229, 495, 613, 2313
BstMAI GTCTC 7 cut(s) 14, 217, 1057, 1433, 2004, 2426, 2564
BstMCI CGRYCG 1 cut(s) 544
BstNI CCWGG 8 cut(s) 692, 736, 743, 1503, 2133, 2742, 2958, 3024
BstNSI RCATGY 2 cut(s) 1030, 1147
BstPAI GACNNNNGTC 1 cut(s) 1896
BstPI GGTNACC 3 cut(s) 1481, 2060, 2144
BstSCI CCNGG 9 cut(s) 690, 734, 741, 1501, 2095, 2131, 2740, 2956, 3022
BstSFI CTRYAG 8 cut(s) 451, 764, 1187, 1395, 1634, 2211, 2940, 3006
BstSLI GKGCMC 4 cut(s) 1858, 2452, 2465, 2956
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 9 cut(s) 459, 463, 1228, 1381, 1409, 1690, 1873, 2992, 3020
BstV2I GAAGAC 2 cut(s) 641, 1383
BstX2I RGATCY 9 cut(s) 787, 1096, 1599, 2050, 2135, 2275, 2836, 2881, 3019
BstXI CCANNNNNNTGG 2 cut(s) 735, 1934
BstYI RGATCY 9 cut(s) 787, 1096, 1599, 2050, 2135, 2275, 2836, 2881, 3019
Bsu15I ATCGAT 1 cut(s) 1680
BsuI GTATCC 2 cut(s) 246, 1567
BsuRI GGCC 4 cut(s) 539, 1501, 2091, 2171
BsuTUI ATCGAT 1 cut(s) 1680
BtgZI GCGATG 1 cut(s) 2822
BtsCI GGATG 7 cut(s) 723, 850, 1048, 2167, 2330, 2503, 2934
BtsI GCAGTG 2 cut(s) 2163, 2684
BtsIMutI CAGTG 8 cut(s) 777, 1462, 2075, 2163, 2465, 2630, 2684, 2970
BveI ACCTGC 4 cut(s) 238, 1140, 1435, 1927
Cac8I GCNNGC 6 cut(s) 681, 1028, 1122, 1854, 2776, 3036
CaiI CAGNNNCTG 1 cut(s) 2603
CfoI GCGC 5 cut(s) 102, 229, 495, 613, 2313
Cfr13I GGNCC 5 cut(s) 537, 2072, 2090, 2642, 2960
ClaI ATCGAT 1 cut(s) 1680
CseI GACGC 1 cut(s) 2665
CspCI CAANNNNNGTGG 2 cut(s) 2139, 2174
DdeI CTNAG 8 cut(s) 231, 299, 927, 1196, 1596, 2001, 2622, 2791
DraI TTTAAA 1 cut(s) 2407
DriI GACNNNNNGTC 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 2169
Eam1104I CTCTTC 2 cut(s) 2184, 2604
Eam1105I GACNNNNNGTC 1 cut(s) 270
EarI CTCTTC 2 cut(s) 2184, 2604
EciI GGCGGA 1 cut(s) 2474
Ecl136II GAGCTC 2 cut(s) 82, 2416
Eco130I CCWWGG 2 cut(s) 2056, 2444
Eco24I GRGCYC 2 cut(s) 84, 2418
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 2 cut(s) 1001, 1054
Eco47I GGWCC 3 cut(s) 2072, 2642, 2960
Eco53kI GAGCTC 2 cut(s) 82, 2416
Eco57I CTGAAG 5 cut(s) 1827, 1836, 2628, 2672, 2859
Eco88I CYCGRG 2 cut(s) 83, 1775
Eco91I GGTNACC 3 cut(s) 1481, 2060, 2144
EcoICRI GAGCTC 2 cut(s) 82, 2416
EcoNI CCTNNNNNAGG 1 cut(s) 1723
EcoO109I RGGNCCY 1 cut(s) 2090
EcoO65I GGTNACC 3 cut(s) 1481, 2060, 2144
EcoRII CCWGG 8 cut(s) 690, 734, 741, 1501, 2131, 2740, 2956, 3022
EcoRV GATATC 2 cut(s) 1001, 1054
EcoT14I CCWWGG 2 cut(s) 2056, 2444
EcoT22I ATGCAT 1 cut(s) 918
EcoT38I GRGCYC 2 cut(s) 84, 2418
ErhI CCWWGG 2 cut(s) 2056, 2444
Esp3I CGTCTC 1 cut(s) 2564
FaqI GGGAC 2 cut(s) 928, 1884
FauI CCCGC 2 cut(s) 1031, 1909
FauNDI CATATG 1 cut(s) 1591
FbaI TGATCA 1 cut(s) 1794
FblI GTMKAC 1 cut(s) 274
FokI GGATG 7 cut(s) 710, 857, 1055, 2174, 2337, 2510, 2921
FriOI GRGCYC 2 cut(s) 84, 2418
FspBI CTAG 5 cut(s) 444, 671, 2423, 2445, 2823
GlaI GCGC 5 cut(s) 101, 228, 494, 612, 2312
GsaI CCCAGC 3 cut(s) 538, 1861, 2567
GsuI CTGGAG 4 cut(s) 661, 725, 1605, 2964
HaeII RGCGCY 2 cut(s) 230, 2314
HaeIII GGCC 4 cut(s) 539, 1501, 2091, 2171
HapII CCGG 3 cut(s) 2048, 2096, 2725
HgaI GACGC 1 cut(s) 2665
HhaI GCGC 5 cut(s) 102, 229, 495, 613, 2313
Hin1I GRCGYC 1 cut(s) 2676
Hin6I GCGC 5 cut(s) 100, 227, 493, 611, 2311
HinP1I GCGC 5 cut(s) 100, 227, 493, 611, 2311
HincII GTYRAC 2 cut(s) 2892, 2932
HindII GTYRAC 2 cut(s) 2892, 2932
HindIII AAGCTT 3 cut(s) 1544, 2552, 2654
HinfI GANTC 5 cut(s) 407, 1294, 1639, 1690, 2479
HpaI GTTAAC 2 cut(s) 2892, 2932
HpaII CCGG 3 cut(s) 2048, 2096, 2725
HphI GGTGA 8 cut(s) 617, 1075, 1475, 1745, 2037, 2072, 2156, 2352
Hpy166II GTNNAC 7 cut(s) 275, 711, 2075, 2801, 2892, 2932, 2954
Hpy188I TCNGA 7 cut(s) 36, 300, 1644, 2289, 2294, 2652, 2794
Hpy8I GTNNAC 7 cut(s) 275, 711, 2075, 2801, 2892, 2932, 2954
HpyAV CCTTC 6 cut(s) 611, 925, 1087, 2052, 2081, 2899
HpyCH4III ACNGT 6 cut(s) 129, 319, 781, 1555, 2460, 2869
HpyCH4IV ACGT 4 cut(s) 568, 826, 1897, 2888
HpyF3I CTNAG 8 cut(s) 231, 299, 927, 1196, 1596, 2001, 2622, 2791
HpySE526I ACGT 4 cut(s) 568, 826, 1897, 2888
Hsp92I GRCGYC 1 cut(s) 2676
HspAI GCGC 5 cut(s) 100, 227, 493, 611, 2311
KpnI GGTACC 1 cut(s) 1914
Ksp22I TGATCA 1 cut(s) 1794
KspAI GTTAAC 2 cut(s) 2892, 2932
LguI GCTCTTC 1 cut(s) 2184
LmnI GCTCC 6 cut(s) 69, 79, 744, 1270, 2413, 2950
Lsp1109I GCAGC 9 cut(s) 459, 463, 1228, 1381, 1409, 1690, 1873, 2992, 3020
LweI GCATC 8 cut(s) 622, 732, 884, 913, 925, 1686, 2152, 2799
MaeI CTAG 5 cut(s) 444, 671, 2423, 2445, 2823
MaeII ACGT 4 cut(s) 568, 826, 1897, 2888
MbiI CCGCTC 1 cut(s) 1780
MfeI CAATTG 2 cut(s) 1301, 2082
MflI RGATCY 9 cut(s) 787, 1096, 1599, 2050, 2135, 2275, 2836, 2881, 3019
MhlI GDGCHC 8 cut(s) 84, 1126, 1858, 2418, 2452, 2465, 2956, 3031
MlyI GAGTC 1 cut(s) 416
Mph1103I ATGCAT 1 cut(s) 918
MroXI GAANNNNTTC 2 cut(s) 2232, 2909
MslI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
MspA1I CMGCKG 2 cut(s) 2567, 3005
MspCI CTTAAG 2 cut(s) 1235, 1720
MspI CCGG 3 cut(s) 2048, 2096, 2725
MspR9I CCNGG 9 cut(s) 692, 736, 743, 1503, 2097, 2133, 2742, 2958, 3024
MunI CAATTG 2 cut(s) 1301, 2082
Mva1269I GAATGC 1 cut(s) 1110
MvaI CCWGG 8 cut(s) 692, 736, 743, 1503, 2133, 2742, 2958, 3024
MvnI CGCG 1 cut(s) 100
NciI CCSGG 1 cut(s) 2097
NdeI CATATG 1 cut(s) 1591
NlaIV GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1912, 2073, 2883, 3021
NmuCI GTSAC 8 cut(s) 197, 262, 1230, 1481, 1885, 2060, 2144, 2531
NsiI ATGCAT 1 cut(s) 918
NspI RCATGY 2 cut(s) 1030, 1147
PaeI GCATGC 1 cut(s) 1030
PaqCI CACCTGC 1 cut(s) 1927
PciSI GCTCTTC 1 cut(s) 2184
PctI GAATGC 1 cut(s) 1110
PdmI GAANNNNTTC 2 cut(s) 2232, 2909
PfeI GAWTC 4 cut(s) 1294, 1639, 1690, 2479
Pfl23II CGTACG 1 cut(s) 95
PflMI CCANNNNNTGG 1 cut(s) 361
Ple19I CGATCG 1 cut(s) 544
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PshAI GACNNNNGTC 1 cut(s) 1896
Psp124BI GAGCTC 2 cut(s) 84, 2418
Psp6I CCWGG 8 cut(s) 690, 734, 741, 1501, 2131, 2740, 2956, 3022
PspEI GGTNACC 3 cut(s) 1481, 2060, 2144
PspFI CCCAGC 3 cut(s) 534, 1857, 2563
PspGI CCWGG 8 cut(s) 690, 734, 741, 1501, 2131, 2740, 2956, 3022
PspLI CGTACG 1 cut(s) 95
PspN4I GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1912, 2073, 2883, 3021
PspPI GGNCC 5 cut(s) 537, 2072, 2090, 2642, 2960
PstI CTGCAG 4 cut(s) 768, 1191, 1399, 3010
PstNI CAGNNNCTG 1 cut(s) 2603
PsuI RGATCY 9 cut(s) 787, 1096, 1599, 2050, 2135, 2275, 2836, 2881, 3019
PvuI CGATCG 1 cut(s) 544
PvuII CAGCTG 2 cut(s) 2567, 3005
RseI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
SacI GAGCTC 2 cut(s) 84, 2418
SapI GCTCTTC 1 cut(s) 2184
Sau96I GGNCC 5 cut(s) 537, 2072, 2090, 2642, 2960
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 9 cut(s) 692, 736, 743, 1503, 2097, 2133, 2742, 2958, 3024
SduI GDGCHC 8 cut(s) 84, 1126, 1858, 2418, 2452, 2465, 2956, 3031
SfaNI GCATC 8 cut(s) 622, 732, 884, 913, 925, 1686, 2152, 2799
SfcI CTRYAG 8 cut(s) 451, 764, 1187, 1395, 1634, 2211, 2940, 3006
SinI GGWCC 3 cut(s) 2072, 2642, 2960
SmiMI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
SmlI CTYRAG 2 cut(s) 1235, 1720
SmoI CTYRAG 2 cut(s) 1235, 1720
SpeI ACTAGT 1 cut(s) 2422
SphI GCATGC 1 cut(s) 1030
SsiI CCGC 7 cut(s) 458, 1024, 1780, 1827, 1902, 1970, 2485
SspI AATATT 5 cut(s) 385, 994, 1656, 2221, 2284
SspMI CTAG 5 cut(s) 444, 671, 2423, 2445, 2823
SstI GAGCTC 2 cut(s) 84, 2418
StyD4I CCNGG 9 cut(s) 690, 734, 741, 1501, 2095, 2131, 2740, 2956, 3022
StyI CCWWGG 2 cut(s) 2056, 2444
TaaI ACNGT 6 cut(s) 129, 319, 781, 1555, 2460, 2869
TaiI ACGT 4 cut(s) 571, 829, 1900, 2891
TaqI TCGA 3 cut(s) 72, 997, 1680
TaqII GACCGA 2 cut(s) 1904, 2519
TatI WGTACW 3 cut(s) 708, 1199, 2798
TauI GCSGC 1 cut(s) 1783
TfiI GAWTC 4 cut(s) 1294, 1639, 1690, 2479
TscAI CASTG 8 cut(s) 784, 1462, 2082, 2163, 2465, 2630, 2691, 2970
TseFI GTSAC 8 cut(s) 197, 262, 1230, 1481, 1885, 2060, 2144, 2531
TseI GCWGC 9 cut(s) 447, 476, 1241, 1394, 1397, 1703, 1861, 3005, 3008
Tsp45I GTSAC 8 cut(s) 197, 262, 1230, 1481, 1885, 2060, 2144, 2531
TspDTI ATGAA 8 cut(s) 10, 297, 360, 579, 1389, 2225, 2286, 2902
TspGWI ACGGA 6 cut(s) 56, 337, 857, 1055, 1337, 3032
TspRI CASTG 8 cut(s) 784, 1462, 2082, 2163, 2465, 2630, 2691, 2970
Van91I CCANNNNNTGG 1 cut(s) 361
Vha464I CTTAAG 2 cut(s) 1235, 1720
VneI GTGCAC 1 cut(s) 2952
VpaK11BI GGWCC 3 cut(s) 2072, 2642, 2960
XagI CCTNNNNNAGG 1 cut(s) 1723
XapI RAATTY 7 cut(s) 398, 968, 1259, 1475, 1769, 2197, 2579
XceI RCATGY 2 cut(s) 1030, 1147
XmaJI CCTAGG 1 cut(s) 2444
XmiI GTMKAC 1 cut(s) 274
XmnI GAANNNNTTC 2 cut(s) 2232, 2909
XspI CTAG 5 cut(s) 444, 671, 2423, 2445, 2823
Zsp2I ATGCAT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.