Rh5DG389200

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007314
Physical Location & Seq
Reverse (-)
60629494 .. 60640617
11124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG389200.1

Sequence Viewer

Length: 3066 bp
ATGGCCTTGTCCGGCATGCGAGGTCTCTCTGTTTTCATCAGCGACATTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTCCGTGTCGATAAGGAGCTCGGGAATGTTCGTACGCGCTTCAAAAACGAAAAGGGTTTGACACCGTATGAGAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGTTGGGATATGATGTGGATTTTGGTCACATGGAAGCAGTCTCTCTTATATCAGCGCCCAAGTATCCAGAAAAGCAGGTTGGGTACATTGTGACATCATGTCTACTCAATGAAAACCACGATTTTCTGAGATTAGCGATCAATACTGTACGGAATGATATTATTGGTCGTAATGAAACTTTCCAATGTTTGGCATTGACTATGGTTGGGAATATTGGAGGCAGAGAATTTGCGGAGTCATTAGCACCTGATGTTCAAAAGTTGCTTATTTCTAGCAGCTACAGACCGCTTGTGAGAAAAAAAGCTGCACTATGTCTTTTGCGCTTGTATAGAAAAAATCCTGATGTTGTCAATATAGATGGCTGGGCCGATCGCATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTGACATCATCTATGAGCCTTCTTGTTGCGCTTGTATCAAATCACCACGATGCTTACTGGAGTTGTCTTCCAAAATGTGTTAAAATACTAGAACGGCTTGCGAGGAACCAGGATATTCCACAGGAGTACACCTACTATGGCATCCCATCTCCCTGGCTCCAGGTCAAGACAATGAGGGTTCTGCAGTATTTCCCCACTGTTGAAGATCCAAATACAAGAAGATCATTGTTTGAGGTCTTACAACGTATATTGATGGGTACGGATGTTGTGAAAAATGTTAATAAGAACAACGCATCACACGCTGTTCTTTTTGAAGCCCTCGCACTTGTCATGCATCTTGATGCTGAGAAGGAGATGATGTCCCAATGTGTTGCACTTCTGGGAAAATTTATTGCTGTGCGAGAACCAAATATTCGATATCTTGGTTTGGAAAATATGACCCGCATGCTAATGGTAACGGATGTGCAAGATATCATCAAGAGACATCAAGCCCAGATTATCACCTCATTGAAGGATCCAGATGTCAGCATTCGTAGGCGTGCTCTTGACCTGCTTTATGGCATGTGTGATGTTTCAAATGCAAAAGACATAGTTGAAGAATTACTGCAGTATCTTAGTACAGCAGACTTCGCAATGCGCGAGGAATTGTCACTTAAGGCTGCCATTCTTGCAGAAAAATTTGCTCCTGATCTATCATGGTATGTAGATGTGATTCTTCAATTGATTGATAAGGCAGGAGATTTTGTTAGTGATGATATATGGTTCCGTGTTGTGCAATTTGTTACGAATAATGAAGACTTACAGCCTTATGCTGCAGCAAAAGCCAGAGAGTATCTTGACAAACCTGCTATACATGAGACAATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGTCACCTTTTAGCCAGACGGCCTGGGTGTAGTCCAAAAGAATTATTTGCCGTCATACATGAGAAGCTTCCTACTGTATCCACTTCTACAATACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAAGAACTACAGAATCAGATTTGGGCAATATTCAATAAATACGAGAGTTGCATCGATGTTGAGATTCAACAACGAGCTGCTGAATACCTTGCCTTAAGTAGGAGGGGTGAAGCTCTTGTTGATATATTGGCTGAAATGCCTAAATTCCCCGAGCGGCAGTCTGCATTGATAAAAAAGGCTGAAGATACTGAAGTTGATACCGCAGAACAAAGTGCTATCAAGTTGCGTGCCCAGCAGCAAACATCAAATGCTTTGGTTGTGACCGACCAACGTCCAGCTAATGGTACCCCACCTGCCAACCACCAGCTTGGTCTTGTGAAGATACCCAACATGAGCAGTAATGCGGATCACAATTCAATCGATCAAGGGTTGTCTCAGGAAAATGGTACTTTGAATAAAGTTGATCCTCAAACTCCTTCACCGGATCTCCTTGGTGACCTCTTGGGTCCACTGGCAATTGAAGGCCCTCCGGGTACTGCCGTTCAATCACATCAAACTGAAATCCCTGGATCTGGTGGTGATCCAAATGCAGTGGATGCTACGGCCATAGTTCCTGTTGGCGAAGAGCCAAATTCTGTTCAGCCTATAGGAAATATTGCAGAAAGATTTCATGCCTTGTGCTTGAAAGATAGTGGCGTTCTGTATGAAGATCCTAATATTCAGATCGGAATTAAAGCAGAGTGGCGCTTACATCAGGGATGTCTTGTTCTTTTCTTGGGAAACAAAAATACTTCACCACTTGTATCAGTTCAGGCTGTAATAATGCCTCCCTCCCACTTTAAAATGGAGCTCTCACTAGTGCCAGAGACTATTCCTCCTAGGGCACAGGTACAGTGCCCCCTTGAAGTTGTGAATCTCCGCCCAAGTAGGGATGTAGCAGTTCTTGACTTCTCTTACAAGTTCGGTCACAATATGGTCAATGTCAAGCTTCGTCTCCCAGCTGTCTTGAATAAATTTCTTCAACCTATACCAGTATCTGCTGAAGAGTTCTTTCCTCAGTGGAGATCACTCACAGGACCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGCGTCAAACCACTGCCACTTGCAGAAATGGCAAATCTAATTAACAGCTTCCGGTTTATGGTTTGTCCAGGGCTTGACCCTAATCCAAATAATCTTGTTGCAAGCACAACCTTTTACTCAGAAAGTACACGGGCGATGCTTTGCTTGGCTAGAATAGAAACAGATCCAGCAGACAGAACCCAGTTGCGTATGACGGTTGCTTCAGGGGATCCTACGTTAACATTTGAGTTGAAGGAGTTCATAAAGGAACAAATAGTTAACATCCCTGTAGCTCCTCGTGCACCTGGACCAGTGCCACCAGCACCTCCAGTAGCTCAACCGACCAGTCCAGCTGCAGCATTAACGGATCCTGGTGCTCTGCTGGCTGGTCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1021

Amino Acids

113.82

Weight (kDa)

5.94

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 2e-116 Adaptin N terminal region
Cnd1 PF12717 125 - 263 1.1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 758 - 854 1.9e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 866 - 975 2.4e-15 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1942
Acc36I ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Acc65I GGTACC 1 cut(s) 1925
AccB1I GGYRCC 1 cut(s) 1925
AccB7I CCANNNNNTGG 2 cut(s) 376, 1922
AccBSI CCGCTC 1 cut(s) 1795
AccI GTMKAC 1 cut(s) 289
AccII CGCG 2 cut(s) 115, 1236
AciI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1985, 2500
AcoI YGGCCR 1 cut(s) 2184
AcsI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
AcuI CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
AcyI GRCGYC 1 cut(s) 2691
AfiI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2153, 2509, 2746
AflII CTTAAG 2 cut(s) 1250, 1735
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 2437
AjnI CCWGG 8 cut(s) 705, 749, 756, 1516, 2146, 2755, 2971, 3037
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
AloI GAACNNNNNNTCC 2 cut(s) 2331, 2363
Alw21I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
Alw26I GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
Alw44I GTGCAC 1 cut(s) 2967
AlwNI CAGNNNCTG 1 cut(s) 2618
Ama87I CYCGRG 2 cut(s) 98, 1790
AoxI GGCC 5 cut(s) 3, 552, 1514, 2104, 2184
ApaLI GTGCAC 1 cut(s) 2967
ApeKI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
ApoI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
Asp700I GAANNNNTTC 2 cut(s) 2247, 2924
Asp718I GGTACC 1 cut(s) 1925
AspA2I CCTAGG 1 cut(s) 2459
AspLEI GCGC 6 cut(s) 117, 244, 510, 628, 1236, 2328
AspS9I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
AsuC2I CCSGG 1 cut(s) 2112
AsuHPI GGTGA 8 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2171, 2367
AvaI CYCGRG 2 cut(s) 98, 1790
AvaII GGWCC 3 cut(s) 2087, 2657, 2975
AvrII CCTAGG 1 cut(s) 2459
BaeGI GKGCMC 4 cut(s) 1873, 2467, 2480, 2971
BaeI ACNNNNGTAYC 8 cut(s) 846, 879, 1190, 1223, 1818, 1851, 2106, 2139
BamHI GGATCC 3 cut(s) 1111, 2896, 3034
BanI GGYRCC 1 cut(s) 1925
BanII GRGCYC 2 cut(s) 99, 2433
BauI CACGAG 1 cut(s) 2964
BbsI GAAGAC 2 cut(s) 656, 1398
Bbv12I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BbvI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BccI CCATC 3 cut(s) 539, 751, 844
BceAI ACGGC 5 cut(s) 707, 1529, 1529, 2105, 2199
BciT130I CCWGG 8 cut(s) 707, 751, 758, 1518, 2148, 2757, 2973, 3039
BciVI GTATCC 2 cut(s) 261, 1582
BcnI CCSGG 1 cut(s) 2112
BcoDI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BcuI ACTAGT 1 cut(s) 2437
BfaI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
BfmI CTRYAG 8 cut(s) 466, 779, 1202, 1410, 1649, 2226, 2955, 3021
BfoI RGCGCY 2 cut(s) 245, 2329
BfrI CTTAAG 2 cut(s) 1250, 1735
BfuAI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BfuI GTATCC 2 cut(s) 261, 1582
BglII AGATCT 1 cut(s) 1614
BlnI CCTAGG 1 cut(s) 2459
Bme1390I CCNGG 9 cut(s) 707, 751, 758, 1518, 2112, 2148, 2757, 2973, 3039
Bme18I GGWCC 3 cut(s) 2087, 2657, 2975
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 2 cut(s) 98, 1790
BmgT120I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
BmiI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BmrFI CCNGG 9 cut(s) 707, 751, 758, 1518, 2112, 2148, 2757, 2973, 3039
BmrI ACTGGG 1 cut(s) 2863
BmsI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
BmuI ACTGGG 1 cut(s) 2863
BoxI GACNNNNGTC 1 cut(s) 1911
BpiI GAAGAC 2 cut(s) 656, 1398
BpmI CTGGAG 4 cut(s) 676, 740, 1620, 2979
BpuMI CCSGG 1 cut(s) 2112
Bsa29I ATCGAT 2 cut(s) 1695, 2001
BsaHI GRCGYC 1 cut(s) 2691
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2756
BsaWI WCCGGW 2 cut(s) 2062, 2739
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2153, 2509, 2746
Bse1I ACTGG 7 cut(s) 659, 2097, 2612, 2869, 2978, 2996, 3012
Bse3DI GCAATG 1 cut(s) 1236
BseBI CCWGG 8 cut(s) 707, 751, 758, 1518, 2148, 2757, 2973, 3039
BseCI ATCGAT 2 cut(s) 1695, 2001
BseDI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2756
BseGI GGATG 7 cut(s) 738, 865, 1063, 2182, 2345, 2518, 2949
BseLI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2153, 2509, 2746
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 5 cut(s) 305, 933, 2030, 2651, 2820
BseNI ACTGG 7 cut(s) 659, 2097, 2612, 2869, 2978, 2996, 3012
BseRI GAGGAG 1 cut(s) 2952
BseSI GKGCMC 4 cut(s) 1873, 2467, 2480, 2971
BseXI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BseYI CCCAGC 3 cut(s) 549, 1872, 2578
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 2 cut(s) 115, 1236
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 5 cut(s) 5, 554, 1516, 2106, 2186
BshNI GGYRCC 1 cut(s) 1925
BshVI ATCGAT 2 cut(s) 1695, 2001
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BsiHKCI CYCGRG 2 cut(s) 98, 1790
BsiSI CCGG 4 cut(s) 12, 2063, 2111, 2740
BsiWI CGTACG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2153, 2509, 2746
BsmAI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BsmBI CGTCTC 1 cut(s) 2579
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 5 cut(s) 5, 554, 1516, 2106, 2186
Bso31I GGTCTC 1 cut(s) 29
BsoBI CYCGRG 2 cut(s) 98, 1790
Bsp1286I GDGCHC 8 cut(s) 99, 1141, 1873, 2433, 2467, 2480, 2971, 3046
BspACI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1985, 2500
BspANI GGCC 5 cut(s) 5, 554, 1516, 2106, 2186
BspCNI CTCAG 5 cut(s) 306, 934, 2029, 2650, 2819
BspDI ATCGAT 2 cut(s) 1695, 2001
BspFNI CGCG 2 cut(s) 115, 1236
BspLI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BspMAI CTGCAG 4 cut(s) 783, 1206, 1414, 3025
BspMI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BspQI GCTCTTC 1 cut(s) 2199
BspT107I GGYRCC 1 cut(s) 1925
BspTI CTTAAG 2 cut(s) 1250, 1735
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 1795
BsrDI GCAATG 1 cut(s) 1236
BsrI ACTGG 7 cut(s) 659, 2097, 2612, 2869, 2978, 2996, 3012
BssECI CCNNGG 6 cut(s) 749, 1517, 2071, 2146, 2459, 2756
BssNI GRCGYC 1 cut(s) 2691
BssSI CACGAG 1 cut(s) 2964
BssT1I CCWWGG 2 cut(s) 2071, 2459
Bst2BI CACGAG 1 cut(s) 2964
Bst2UI CCWGG 8 cut(s) 707, 751, 758, 1518, 2148, 2757, 2973, 3039
Bst4CI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
Bst6I CTCTTC 2 cut(s) 2199, 2619
BstACI GRCGYC 1 cut(s) 2691
BstAFI CTTAAG 2 cut(s) 1250, 1735
BstAPI GCANNNNNTGC 1 cut(s) 2177
BstC8I GCNNGC 7 cut(s) 17, 696, 1043, 1137, 1869, 2791, 3051
BstDEI CTNAG 7 cut(s) 314, 942, 1211, 1611, 2016, 2637, 2806
BstEII GGTNACC 2 cut(s) 1496, 2075
BstENI CCTNNNNNAGG 1 cut(s) 1738
BstF5I GGATG 7 cut(s) 738, 865, 1063, 2182, 2345, 2518, 2949
BstFNI CGCG 2 cut(s) 115, 1236
BstH2I RGCGCY 2 cut(s) 245, 2329
BstHHI GCGC 6 cut(s) 117, 244, 510, 628, 1236, 2328
BstMAI GTCTC 7 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579
BstMCI CGRYCG 1 cut(s) 559
BstNI CCWGG 8 cut(s) 707, 751, 758, 1518, 2148, 2757, 2973, 3039
BstNSI RCATGY 3 cut(s) 19, 1045, 1162
BstPAI GACNNNNGTC 1 cut(s) 1911
BstPI GGTNACC 2 cut(s) 1496, 2075
BstSCI CCNGG 9 cut(s) 705, 749, 756, 1516, 2110, 2146, 2755, 2971, 3037
BstSFI CTRYAG 8 cut(s) 466, 779, 1202, 1410, 1649, 2226, 2955, 3021
BstSLI GKGCMC 4 cut(s) 1873, 2467, 2480, 2971
BstUI CGCG 2 cut(s) 115, 1236
BstV1I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BstV2I GAAGAC 2 cut(s) 656, 1398
BstX2I RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
BstXI CCANNNNNNTGG 2 cut(s) 750, 1949
BstYI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
Bsu15I ATCGAT 2 cut(s) 1695, 2001
BsuI GTATCC 2 cut(s) 261, 1582
BsuRI GGCC 5 cut(s) 5, 554, 1516, 2106, 2186
BsuTUI ATCGAT 2 cut(s) 1695, 2001
BtgZI GCGATG 1 cut(s) 2837
BtsCI GGATG 7 cut(s) 738, 865, 1063, 2182, 2345, 2518, 2949
BtsI GCAGTG 2 cut(s) 2178, 2699
BtsIMutI CAGTG 8 cut(s) 792, 1477, 2090, 2178, 2480, 2645, 2699, 2985
BveI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Cac8I GCNNGC 7 cut(s) 17, 696, 1043, 1137, 1869, 2791, 3051
CaiI CAGNNNCTG 1 cut(s) 2618
CfoI GCGC 6 cut(s) 117, 244, 510, 628, 1236, 2328
Cfr13I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
ClaI ATCGAT 2 cut(s) 1695, 2001
CseI GACGC 1 cut(s) 2680
CspCI CAANNNNNGTGG 2 cut(s) 2154, 2189
DdeI CTNAG 7 cut(s) 314, 942, 1211, 1611, 2016, 2637, 2806
DraI TTTAAA 1 cut(s) 2422
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 2184
Eam1104I CTCTTC 2 cut(s) 2199, 2619
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 2 cut(s) 2199, 2619
EciI GGCGGA 1 cut(s) 2489
Ecl136II GAGCTC 2 cut(s) 97, 2431
Eco130I CCWWGG 2 cut(s) 2071, 2459
Eco24I GRGCYC 2 cut(s) 99, 2433
Eco31I GGTCTC 1 cut(s) 29
Eco32I GATATC 2 cut(s) 1016, 1069
Eco47I GGWCC 3 cut(s) 2087, 2657, 2975
Eco53kI GAGCTC 2 cut(s) 97, 2431
Eco57I CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
Eco88I CYCGRG 2 cut(s) 98, 1790
Eco91I GGTNACC 2 cut(s) 1496, 2075
EcoICRI GAGCTC 2 cut(s) 97, 2431
EcoNI CCTNNNNNAGG 1 cut(s) 1738
EcoO109I RGGNCCY 1 cut(s) 2105
EcoO65I GGTNACC 2 cut(s) 1496, 2075
EcoRII CCWGG 8 cut(s) 705, 749, 756, 1516, 2146, 2755, 2971, 3037
EcoRV GATATC 2 cut(s) 1016, 1069
EcoT14I CCWWGG 2 cut(s) 2071, 2459
EcoT22I ATGCAT 1 cut(s) 933
EcoT38I GRGCYC 2 cut(s) 99, 2433
ErhI CCWWGG 2 cut(s) 2071, 2459
Esp3I CGTCTC 1 cut(s) 2579
FaqI GGGAC 1 cut(s) 943
FauI CCCGC 1 cut(s) 1046
FauNDI CATATG 1 cut(s) 1606
FblI GTMKAC 1 cut(s) 289
FokI GGATG 7 cut(s) 725, 872, 1070, 2189, 2352, 2525, 2936
FriOI GRGCYC 2 cut(s) 99, 2433
FspBI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
GlaI GCGC 6 cut(s) 116, 243, 509, 627, 1235, 2327
GsaI CCCAGC 3 cut(s) 553, 1876, 2582
GsuI CTGGAG 4 cut(s) 676, 740, 1620, 2979
HaeII RGCGCY 2 cut(s) 245, 2329
HaeIII GGCC 5 cut(s) 5, 554, 1516, 2106, 2186
HapII CCGG 4 cut(s) 12, 2063, 2111, 2740
HgaI GACGC 1 cut(s) 2680
HhaI GCGC 6 cut(s) 117, 244, 510, 628, 1236, 2328
Hin1I GRCGYC 1 cut(s) 2691
Hin6I GCGC 6 cut(s) 115, 242, 508, 626, 1234, 2326
HinP1I GCGC 6 cut(s) 115, 242, 508, 626, 1234, 2326
HincII GTYRAC 2 cut(s) 2907, 2947
HindII GTYRAC 2 cut(s) 2907, 2947
HindIII AAGCTT 3 cut(s) 1559, 2567, 2669
HinfI GANTC 5 cut(s) 422, 1309, 1654, 1705, 2494
HpaI GTTAAC 2 cut(s) 2907, 2947
HpaII CCGG 4 cut(s) 12, 2063, 2111, 2740
HphI GGTGA 8 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2171, 2367
Hpy166II GTNNAC 7 cut(s) 290, 726, 2090, 2816, 2907, 2947, 2969
Hpy188I TCNGA 7 cut(s) 51, 315, 1659, 2304, 2309, 2667, 2809
Hpy8I GTNNAC 7 cut(s) 290, 726, 2090, 2816, 2907, 2947, 2969
HpyAV CCTTC 6 cut(s) 626, 940, 1102, 2067, 2096, 2914
HpyCH4III ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
HpyCH4IV ACGT 4 cut(s) 583, 841, 1912, 2903
HpyF3I CTNAG 7 cut(s) 314, 942, 1211, 1611, 2016, 2637, 2806
HpySE526I ACGT 4 cut(s) 583, 841, 1912, 2903
Hsp92I GRCGYC 1 cut(s) 2691
HspAI GCGC 6 cut(s) 115, 242, 508, 626, 1234, 2326
KpnI GGTACC 1 cut(s) 1929
KspAI GTTAAC 2 cut(s) 2907, 2947
LguI GCTCTTC 1 cut(s) 2199
LmnI GCTCC 6 cut(s) 84, 94, 759, 1285, 2428, 2965
Lsp1109I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
LweI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
MaeI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
MaeII ACGT 4 cut(s) 583, 841, 1912, 2903
MaeIII GTNAC 9 cut(s) 212, 277, 1051, 1245, 1378, 1496, 1900, 2075, 2546
MbiI CCGCTC 1 cut(s) 1795
MfeI CAATTG 2 cut(s) 1316, 2097
MflI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
MhlI GDGCHC 8 cut(s) 99, 1141, 1873, 2433, 2467, 2480, 2971, 3046
MlyI GAGTC 1 cut(s) 431
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 2 cut(s) 2247, 2924
MslI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
MspA1I CMGCKG 2 cut(s) 2582, 3020
MspCI CTTAAG 2 cut(s) 1250, 1735
MspI CCGG 4 cut(s) 12, 2063, 2111, 2740
MspR9I CCNGG 9 cut(s) 707, 751, 758, 1518, 2112, 2148, 2757, 2973, 3039
MunI CAATTG 2 cut(s) 1316, 2097
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 8 cut(s) 707, 751, 758, 1518, 2148, 2757, 2973, 3039
MvnI CGCG 2 cut(s) 115, 1236
NciI CCSGG 1 cut(s) 2112
NdeI CATATG 1 cut(s) 1606
NlaIV GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
NmuCI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 3 cut(s) 19, 1045, 1162
PaeI GCATGC 2 cut(s) 19, 1045
PaqCI CACCTGC 1 cut(s) 1942
PciSI GCTCTTC 1 cut(s) 2199
PcsI WCGNNNNNNNCGW 1 cut(s) 1233
PctI GAATGC 1 cut(s) 1125
PdmI GAANNNNTTC 2 cut(s) 2247, 2924
PfeI GAWTC 4 cut(s) 1309, 1654, 1705, 2494
Pfl23II CGTACG 1 cut(s) 110
PflMI CCANNNNNTGG 2 cut(s) 376, 1922
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1911
Psp124BI GAGCTC 2 cut(s) 99, 2433
Psp6I CCWGG 8 cut(s) 705, 749, 756, 1516, 2146, 2755, 2971, 3037
PspEI GGTNACC 2 cut(s) 1496, 2075
PspFI CCCAGC 3 cut(s) 549, 1872, 2578
PspGI CCWGG 8 cut(s) 705, 749, 756, 1516, 2146, 2755, 2971, 3037
PspLI CGTACG 1 cut(s) 110
PspN4I GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
PspPI GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
PstI CTGCAG 4 cut(s) 783, 1206, 1414, 3025
PstNI CAGNNNCTG 1 cut(s) 2618
PsuI RGATCY 9 cut(s) 802, 1111, 1614, 2065, 2150, 2290, 2851, 2896, 3034
PvuI CGATCG 1 cut(s) 559
PvuII CAGCTG 2 cut(s) 2582, 3020
RseI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SacI GAGCTC 2 cut(s) 99, 2433
SapI GCTCTTC 1 cut(s) 2199
Sau96I GGNCC 5 cut(s) 552, 2087, 2105, 2657, 2975
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 9 cut(s) 707, 751, 758, 1518, 2112, 2148, 2757, 2973, 3039
SduI GDGCHC 8 cut(s) 99, 1141, 1873, 2433, 2467, 2480, 2971, 3046
SfaNI GCATC 8 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814
SfcI CTRYAG 8 cut(s) 466, 779, 1202, 1410, 1649, 2226, 2955, 3021
SinI GGWCC 3 cut(s) 2087, 2657, 2975
SmiMI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SmlI CTYRAG 2 cut(s) 1250, 1735
SmoI CTYRAG 2 cut(s) 1250, 1735
SpeI ACTAGT 1 cut(s) 2437
SphI GCATGC 2 cut(s) 19, 1045
SsiI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1985, 2500
SspI AATATT 5 cut(s) 400, 1009, 1671, 2236, 2299
SspMI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
SstI GAGCTC 2 cut(s) 99, 2433
StyD4I CCNGG 9 cut(s) 705, 749, 756, 1516, 2110, 2146, 2755, 2971, 3037
StyI CCWWGG 2 cut(s) 2071, 2459
TaaI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
TaiI ACGT 4 cut(s) 586, 844, 1915, 2906
TaqI TCGA 4 cut(s) 87, 1012, 1695, 2001
TaqII GACCGA 2 cut(s) 1919, 2534
TatI WGTACW 3 cut(s) 723, 1214, 2813
TauI GCSGC 1 cut(s) 1798
TfiI GAWTC 4 cut(s) 1309, 1654, 1705, 2494
TscAI CASTG 8 cut(s) 799, 1477, 2097, 2178, 2480, 2645, 2706, 2985
TseFI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TseI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
Tsp45I GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TspDTI ATGAA 8 cut(s) 25, 312, 375, 594, 1404, 2240, 2301, 2917
TspGWI ACGGA 6 cut(s) 71, 352, 872, 1070, 1352, 3047
TspRI CASTG 8 cut(s) 799, 1477, 2097, 2178, 2480, 2645, 2706, 2985
Van91I CCANNNNNTGG 2 cut(s) 376, 1922
Vha464I CTTAAG 2 cut(s) 1250, 1735
VneI GTGCAC 1 cut(s) 2967
VpaK11BI GGWCC 3 cut(s) 2087, 2657, 2975
XagI CCTNNNNNAGG 1 cut(s) 1738
XapI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
XceI RCATGY 3 cut(s) 19, 1045, 1162
XmaJI CCTAGG 1 cut(s) 2459
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 2247, 2924
XspI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.