AT5G22780

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007314
Physical Location & Seq
Reverse (-)
7589403 .. 7598113
8711 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22780.2

Sequence Viewer

Length: 3042 bp
ATGACCGGAATGAGAGGTCTCTCCGTATTTATCAGCGACGTTCGTAATTGTCAGAACAAGGAAGCGGAGAGACTACGAGTTGATAAGGAGCTCGGAAACATTCGTACATGCTTCAAGAATGAGAAGGTCTTAACACCATACAAAAAGAAGAAATATGTCTGGAAAATGCTTTATATACATATGCTAGGCTACGATGTGGATTTTGGCCATATGGAGGCAGTTTCTCTTATATCTGCGCCAAAGTATCCAGAAAAGCAGGTTGGCTACATTGTTACATCTTGCTTGCTCAATGAGAACCATGACTTTTTGAAGTTGGCTATAAATACAGTGCGGAACGATATTATTGGTCGGAATGAGACTTTCCAATGCTTAGCACTGACCTTGGTTGGAAATATTGGTGGAAGAGATTTTGCTGAATCATTGGCACCTGATGTTCAAAAGCTACTTATTTCTAGTAGTTGCAGACCTCTCGTAAGGAAAAAAGCGGCACTGTGCCTACTGCGCTTATTCCGAAAAAATCCGGATGCTGTCAACGTTGATGGCTGGGCGGATCGTATGGCACAACTTTTGGATGAACGAGATCTTGGTGTCTTGACGTCTTCTACGAGTCTTCTTGTAGCTCTGGTATCTAACAATCATGAGGCGTACTCAAGTTGTCTACCTAAATGTGTCAAAATATTAGAACGCCTTGCTAGGAACCAGGATGTTCCTCAGGAGTACACATACTATGGCATCCCATCTCCTTGGCTCCAGGTTAAGGCAATGAGGGCTCTTCAATATTTTCCAACCATTGAAGATCCCAGTACCAGAAAAGCTCTCTTTGAGGTCCTTCAGCGAATATTGATGGGCACAGATGTTGTGAAAAATGTGAATAAAAACAATGCGTCTCATGCCGTGCTGTTTGAAGCTCTCTCCCTGGTCATGCATCTTGATGCTGAGAAGGAAATGATGTCACAGTGTGTTGCTCTTCTTGGCAAGTTTATTTCTGTCCGTGAGCCCAACATCAGATATCTAGGCTTGGAAAATATGACGCGAATGCTGATGGTCACAGATGTTCAAGATATCATAAAGAAGCATCAGTCTCAGATAATCACCTCGTTGAAAGACCCTGACATCAGTATTCGTAGACGCGCTCTTGATTTGCTATATGGGATGTGCGATGTGTCAAATGCAAAGGATATAGTTGAAGAGTTGTTGCAGTATCTTAGCACAGCAGAATTTTCTATGCGGGAGGAATTATCACTTAAAGCTGCTATTCTTGCTGAGAAGTTTGCTCCTGATCTTTCATGGTATGTAGATGTGATCCTTCAGTTGATCGACAAGGCTGGGGATTTTGTTAGCGATGACATTTGGTTTCGTGTGGTTCAGTTTGTAACAAATAATGAAGACCTGCAGCCTTATGCGGCATCAAAAGCAAGAGAATATATGGACAAAATTGCAATCCATGAGACCATGGTGAAGGTCAGTGCTTATATTTTGGGGGAGTATGGTCACCTTTTAGCTAGACAACCTGGATGCAGTGCAAGTGAACTCTTTAGCATCTTACATGAGAAGCTTCCTACAGTATCGACTCCCACCATTCCTATTCTTCTTTCTACATATGCAAAGCTTCTTATGCACGCTCAACCCCCGGATCCAGAACTGCAAAAAAAAGTTTGGGCTGTATTTAAGAAATATGAGAGTTGTATCGATGTGGAGATACAACAGAGGGCAGTTGAATATTTTGAATTGAGTAAAAAAGGGCCTGCCTTTATGGATGTATTGGCTGAGATGCCAAAATTTCCGGAGCGCCAGTCTTCATTGATTAAAAAGGCAGAAAACGTTGAAGATACCGCAGACCAGAGTGCCATCAAACTTAGAGCACAACAACAGCCTTCAAATGCTATTGTTTTGGCTGACCCACAGCCTGTTAATGGGGCACCACCACCTTTGAAGGTTCCAATTTTAAGTGGAAGCACGGACCCTGAGTCAGTGGCTCGATCTCTCTCACATCCCAATGGAACTTTAAGTAATATAGATCCACAAACTCCATCACCTGATCTCCTTAGTGACCTACTCGGTCCTCTTGCAATAGAGGCTCCACCTGGAGCTGTCTCATATGAACAACATGGTCCCGTAGGGGCAGAAGGAGTTCCAGATGAAATTGATGGTTCCGCCATTGTACCTGTTGAAGAGCAAACAAATACAGTGGAGCTTATCGGAAATATTGCTGAGAGATTTCATGCTTTATGTTTGAAAGACAGTGGTGTCTTATACGAGGATCCTCATATCCAGATTGGCATCAAAGCTGAATGGAGAGGACACCATGGGAGACTTGTTCTCTTCATGGGAAACAAGAATACTTCTCCACTTACATCAGTTCAAGCTCTGATACTACCCCCTGCCCACTTAAGACTGGATCTGTCTCCAGTACCTGATACAATTCCTCCTCGAGCACAGGTACAATCTCCGCTTGAAGTGATGAACATCCGCCCTAGTAGGGATGTTGCAGTTTTGGATTTCTCCTACAAGTTTGGTACAAACGTGGTCAGTGCCAAATTACGCATCCCGGCAACCTTGAATAAGTTTCTTCAACCTCTACAATTGACATCTGAAGAGTTCTTCCCTCAATGGAGAGCAATCTCAGGGCCACCCTTGAAACTTCAGGAAGTGGTAAGAGGTGTAAGACCTCTAGCTCTTCCGGAAATGGCAAATCTGTTCAACAGTTTTCATGTGACGATATGCCCTGGGCTTGATCCAAACCCTAATAATCTAGTGGCAAGCACAACCTTCTACTCTGAAACTACAGGAGCCATGCTCTGTTTGGCAAGAATTGAAACAGACCCAGCGGACAGAACCCAACTACGATTGACAGTAGGCTCGGGAGATCCTACCCTTACATTTGAGTTAAAAGAATTCATCAAGGAACAATTAATTACTATTCCAATGGGATCTCGAGCTCTAGTTCCAGCAGCAGGTCCAGCACCATCACCAGCAGTTCAACCGCCTAGCCCAGCGGCTTTAGCTGATGATCCAGGAGCTATGCTTGCTGGCTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1013

Amino Acids

112.3

Weight (kDa)

5.85

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 580 6.2e-117 Adaptin N terminal region
Cnd1 PF12717 123 - 261 3.1e-07 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 748 - 842 1.7e-16 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 856 - 965 1.2e-16 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2246
AatII GACGTC 1 cut(s) 599
Acc36I ACCTGC 3 cut(s) 247, 1398, 2945
AccB1I GGYRCC 2 cut(s) 424, 1918
AccI GTMKAC 2 cut(s) 658, 1126
AccII CGCG 2 cut(s) 1033, 1131
AccIII TCCGGA 3 cut(s) 520, 1783, 2680
AclI AACGTT 2 cut(s) 534, 1821
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 1217, 1778, 2894
AcuI CTGAAG 4 cut(s) 815, 1292, 2613, 2627
AcyI GRCGYC 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 959
AfaI GTAC 8 cut(s) 106, 647, 719, 805, 2163, 2412, 2442, 2518
AfiI CCNNNNNNNGG 5 cut(s) 214, 757, 1913, 2479, 2954
AflII CTTAAG 1 cut(s) 2389
AhdI GACNNNNNGTC 1 cut(s) 1966
AjnI CCWGG 7 cut(s) 699, 750, 915, 1510, 2083, 2725, 3013
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 567, 599
Alw21I GWGCWC 4 cut(s) 93, 1864, 2437, 2941
Alw26I GTCTC 9 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2305, 2409
AlwNI CAGNNNCTG 1 cut(s) 2414
Ama87I CYCGRG 3 cut(s) 2430, 2860, 2934
Aor13HI TCCGGA 3 cut(s) 520, 1783, 2680
AoxI GGCC 3 cut(s) 205, 1742, 2627
ApeKI GCWGC 3 cut(s) 1250, 1393, 2951
ApoI RAATTY 3 cut(s) 1217, 1778, 2894
AseI ATTAAT 1 cut(s) 2912
Asp700I GAANNNNTTC 1 cut(s) 2130
AspLEI GCGC 4 cut(s) 238, 504, 1133, 1791
AspS9I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
AsuC2I CCSGG 2 cut(s) 1631, 2549
AsuHPI GGTGA 5 cut(s) 1084, 1468, 1484, 2025, 2961
AvaI CYCGRG 3 cut(s) 2430, 2860, 2934
AvaII GGWCC 5 cut(s) 826, 1960, 2060, 2111, 2957
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 2 cut(s) 851, 1921
BaeI ACNNNNGTAYC 2 cut(s) 2508, 2541
BalI TGGCCA 1 cut(s) 207
BamHI GGATCC 2 cut(s) 1633, 2260
BanI GGYRCC 2 cut(s) 424, 1918
BanII GRGCYC 4 cut(s) 93, 772, 999, 2941
BbsI GAAGAC 4 cut(s) 591, 602, 1392, 1788
Bbv12I GWGCWC 4 cut(s) 93, 1864, 2437, 2941
BbvI GCAGC 3 cut(s) 1237, 1405, 2963
BccI CCATC 8 cut(s) 533, 745, 838, 1036, 1856, 2038, 2141, 2974
BceAI ACGGC 1 cut(s) 878
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciT130I CCWGG 7 cut(s) 701, 752, 917, 1512, 2085, 2727, 3015
BciVI GTATCC 1 cut(s) 255
BcnI CCSGG 2 cut(s) 1631, 2549
BcoDI GTCTC 9 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2305, 2409
BfmI CTRYAG 3 cut(s) 1391, 1560, 2784
BfoI RGCGCY 1 cut(s) 1792
BfrI CTTAAG 1 cut(s) 2389
BfuAI ACCTGC 3 cut(s) 247, 1398, 2945
BfuI GTATCC 1 cut(s) 255
BglII AGATCT 1 cut(s) 580
BisI GCNGC 6 cut(s) 486, 1251, 1394, 1404, 2952, 2997
BlpI GCTNAGC 1 cut(s) 370
BlsI GCNGC 6 cut(s) 487, 1252, 1395, 1405, 2953, 2998
Bme1390I CCNGG 9 cut(s) 701, 752, 917, 1512, 1631, 2085, 2549, 2727, 3015
Bme18I GGWCC 5 cut(s) 826, 1960, 2060, 2111, 2957
BmeRI GACNNNNNGTC 1 cut(s) 1966
BmeT110I CYCGRG 3 cut(s) 2430, 2860, 2934
BmgT120I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
BmrFI CCNGG 9 cut(s) 701, 752, 917, 1512, 1631, 2085, 2549, 2727, 3015
BmrI ACTGGG 1 cut(s) 795
BmuI ACTGGG 1 cut(s) 795
BpiI GAAGAC 4 cut(s) 591, 602, 1392, 1788
BplI GAGNNNNNCTC 4 cut(s) 632, 664, 2781, 2813
BpmI CTGGAG 3 cut(s) 734, 2106, 2391
Bpu1102I GCTNAGC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 2 cut(s) 1631, 2549
Bsa29I ATCGAT 1 cut(s) 1689
BsaBI GATNNNNATC 2 cut(s) 2279, 2465
BsaHI GRCGYC 1 cut(s) 596
BsaI GGTCTC 2 cut(s) 23, 1442
BsaJI CCNNGG 8 cut(s) 381, 743, 915, 1452, 1629, 2305, 2725, 2726
BsaWI WCCGGW 4 cut(s) 5, 520, 1783, 2680
BsaXI ACNNNNNCTCC 6 cut(s) 1778, 1808, 2025, 2055, 2410, 2440
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 757, 1913, 2479, 2954
Bse1I ACTGG 4 cut(s) 801, 1792, 2400, 2408
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 768
Bse8I GATNNNNATC 2 cut(s) 2279, 2465
BseAI TCCGGA 3 cut(s) 520, 1783, 2680
BseBI CCWGG 7 cut(s) 701, 752, 917, 1512, 2085, 2727, 3015
BseCI ATCGAT 1 cut(s) 1689
BseDI CCNNGG 8 cut(s) 381, 743, 915, 1452, 1629, 2305, 2725, 2726
BseJI GATNNNNATC 2 cut(s) 2279, 2465
BseLI CCNNNNNNNGG 5 cut(s) 214, 757, 1913, 2479, 2954
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 8 cut(s) 725, 927, 1097, 1254, 1758, 1956, 2202, 2637
BseNI ACTGG 4 cut(s) 801, 1792, 2400, 2408
BseRI GAGGAG 1 cut(s) 2418
BseSI GKGCMC 2 cut(s) 851, 1921
BseXI GCAGC 3 cut(s) 1237, 1405, 2963
BseYI CCCAGC 4 cut(s) 543, 1325, 2824, 2992
Bsh1236I CGCG 2 cut(s) 1033, 1131
BshFI GGCC 3 cut(s) 207, 1744, 2629
BshNI GGYRCC 2 cut(s) 424, 1918
BshVI ATCGAT 1 cut(s) 1689
BsiHKAI GWGCWC 4 cut(s) 93, 1864, 2437, 2941
BsiHKCI CYCGRG 3 cut(s) 2430, 2860, 2934
BsiSI CCGG 6 cut(s) 6, 521, 1631, 1784, 2549, 2681
BslFI GGGAC 1 cut(s) 2097
BslI CCNNNNNNNGG 5 cut(s) 214, 757, 1913, 2479, 2954
BsmAI GTCTC 9 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2305, 2409
BsmBI CGTCTC 1 cut(s) 891
BsmFI GGGAC 1 cut(s) 2097
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 3 cut(s) 207, 1744, 2629
Bso31I GGTCTC 2 cut(s) 23, 1442
BsoBI CYCGRG 3 cut(s) 2430, 2860, 2934
Bsp1286I GDGCHC 8 cut(s) 93, 772, 851, 999, 1864, 1921, 2437, 2941
Bsp13I TCCGGA 3 cut(s) 520, 1783, 2680
Bsp1720I GCTNAGC 1 cut(s) 370
Bsp19I CCATGG 2 cut(s) 1452, 2305
BspANI GGCC 3 cut(s) 207, 1744, 2629
BspCNI CTCAG 8 cut(s) 724, 928, 1096, 1255, 1759, 1957, 2203, 2636
BspDI ATCGAT 1 cut(s) 1689
BspEI TCCGGA 3 cut(s) 520, 1783, 2680
BspFNI CGCG 2 cut(s) 1033, 1131
BspHI TCATGA 1 cut(s) 637
BspMAI CTGCAG 1 cut(s) 1395
BspMI ACCTGC 3 cut(s) 247, 1398, 2945
BspQI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
BspT107I GGYRCC 2 cut(s) 424, 1918
BspTI CTTAAG 1 cut(s) 2389
BspTNI GGTCTC 2 cut(s) 23, 1442
BsrDI GCAATG 1 cut(s) 768
BsrI ACTGG 4 cut(s) 801, 1792, 2400, 2408
BssECI CCNNGG 8 cut(s) 381, 743, 915, 1452, 1629, 2305, 2725, 2726
BssNI GRCGYC 1 cut(s) 596
BssT1I CCWWGG 4 cut(s) 381, 743, 1452, 2305
Bst2UI CCWGG 7 cut(s) 701, 752, 917, 1512, 2085, 2727, 3015
Bst4CI ACNGT 8 cut(s) 328, 492, 957, 1564, 2188, 2243, 2705, 2854
Bst6I CTCTTC 8 cut(s) 397, 777, 972, 1182, 2166, 2327, 2589, 2682
BstACI GRCGYC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 2389
BstC8I GCNNGC 6 cut(s) 284, 1620, 1746, 2761, 3027, 3031
BstDSI CCRYGG 2 cut(s) 1452, 2305
BstEII GGTNACC 1 cut(s) 1490
BstFNI CGCG 2 cut(s) 1033, 1131
BstH2I RGCGCY 1 cut(s) 1792
BstHHI GCGC 4 cut(s) 238, 504, 1133, 1791
BstMAI GTCTC 9 cut(s) 23, 64, 350, 891, 1086, 1442, 2098, 2305, 2409
BstNI CCWGG 7 cut(s) 701, 752, 917, 1512, 2085, 2727, 3015
BstNSI RCATGY 1 cut(s) 111
BstPI GGTNACC 1 cut(s) 1490
BstSCI CCNGG 9 cut(s) 699, 750, 915, 1510, 1629, 2083, 2547, 2725, 3013
BstSFI CTRYAG 3 cut(s) 1391, 1560, 2784
BstSLI GKGCMC 2 cut(s) 851, 1921
BstUI CGCG 2 cut(s) 1033, 1131
BstV1I GCAGC 3 cut(s) 1237, 1405, 2963
BstV2I GAAGAC 4 cut(s) 591, 602, 1392, 1788
BstX2I RGATCY 8 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2866, 2930
BstXI CCANNNNNNTGG 1 cut(s) 744
BstYI RGATCY 8 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2866, 2930
Bsu15I ATCGAT 1 cut(s) 1689
Bsu36I CCTNAGG 1 cut(s) 711
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 3 cut(s) 207, 1744, 2629
BsuTUI ATCGAT 1 cut(s) 1689
BtgI CCRYGG 2 cut(s) 1452, 2305
BtgZI GCGATG 2 cut(s) 1173, 1356
BtsI GCAGTG 1 cut(s) 1525
BveI ACCTGC 3 cut(s) 247, 1398, 2945
Cac8I GCNNGC 6 cut(s) 284, 1620, 1746, 2761, 3027, 3031
CaiI CAGNNNCTG 1 cut(s) 2414
CciI TCATGA 1 cut(s) 637
CfoI GCGC 4 cut(s) 238, 504, 1133, 1791
Cfr13I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
ClaI ATCGAT 1 cut(s) 1689
CseI GACGC 3 cut(s) 873, 1039, 1137
Csp6I GTAC 8 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2517
CspCI CAANNNNNGTGG 4 cut(s) 1911, 1946, 2169, 2204
CviQI GTAC 8 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2517
DraIII CACNNNGTG 1 cut(s) 959
DrdI GACNNNNNNGTC 1 cut(s) 2246
DriI GACNNNNNGTC 1 cut(s) 1966
DseDI GACNNNNNNGTC 1 cut(s) 2246
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 8 cut(s) 397, 777, 972, 1182, 2166, 2327, 2589, 2682
Eam1105I GACNNNNNGTC 1 cut(s) 1966
EarI CTCTTC 8 cut(s) 397, 777, 972, 1182, 2166, 2327, 2589, 2682
EciI GGCGGA 3 cut(s) 563, 2143, 2459
Ecl136II GAGCTC 2 cut(s) 91, 2939
Eco130I CCWWGG 4 cut(s) 381, 743, 1452, 2305
Eco24I GRGCYC 4 cut(s) 93, 772, 999, 2941
Eco31I GGTCTC 2 cut(s) 23, 1442
Eco32I GATATC 2 cut(s) 1010, 1063
Eco47I GGWCC 5 cut(s) 826, 1960, 2060, 2111, 2957
Eco53kI GAGCTC 2 cut(s) 91, 2939
Eco57I CTGAAG 4 cut(s) 815, 1292, 2613, 2627
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 3 cut(s) 2430, 2860, 2934
Eco91I GGTNACC 1 cut(s) 1490
EcoICRI GAGCTC 2 cut(s) 91, 2939
EcoO109I RGGNCCY 2 cut(s) 826, 1742
EcoO65I GGTNACC 1 cut(s) 1490
EcoRI GAATTC 1 cut(s) 2894
EcoRII CCWGG 7 cut(s) 699, 750, 915, 1510, 2083, 2725, 3013
EcoRV GATATC 2 cut(s) 1010, 1063
EcoT14I CCWWGG 4 cut(s) 381, 743, 1452, 2305
EcoT22I ATGCAT 1 cut(s) 927
EcoT38I GRGCYC 4 cut(s) 93, 772, 999, 2941
ErhI CCWWGG 4 cut(s) 381, 743, 1452, 2305
Esp3I CGTCTC 1 cut(s) 891
FaqI GGGAC 1 cut(s) 2097
FauI CCCGC 1 cut(s) 1221
FauNDI CATATG 4 cut(s) 180, 210, 1600, 2098
FblI GTMKAC 2 cut(s) 658, 1126
Fnu4HI GCNGC 6 cut(s) 486, 1251, 1394, 1404, 2952, 2997
FriOI GRGCYC 4 cut(s) 93, 772, 999, 2941
Fsp4HI GCNGC 6 cut(s) 486, 1251, 1394, 1404, 2952, 2997
GlaI GCGC 4 cut(s) 237, 503, 1132, 1790
GluI GCNGC 6 cut(s) 486, 1251, 1394, 1404, 2952, 2997
GsaI CCCAGC 4 cut(s) 547, 1329, 2828, 2996
GsuI CTGGAG 3 cut(s) 734, 2106, 2391
HaeII RGCGCY 1 cut(s) 1792
HaeIII GGCC 3 cut(s) 207, 1744, 2629
HapII CCGG 6 cut(s) 6, 521, 1631, 1784, 2549, 2681
HgaI GACGC 3 cut(s) 873, 1039, 1137
HhaI GCGC 4 cut(s) 238, 504, 1133, 1791
Hin1I GRCGYC 1 cut(s) 596
Hin6I GCGC 4 cut(s) 236, 502, 1131, 1789
HinP1I GCGC 4 cut(s) 236, 502, 1131, 1789
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 2 cut(s) 1553, 1607
HinfI GANTC 4 cut(s) 416, 607, 1570, 1967
HpaII CCGG 6 cut(s) 6, 521, 1631, 1784, 2549, 2681
HphI GGTGA 5 cut(s) 1084, 1468, 1484, 2025, 2961
Hpy166II GTNNAC 5 cut(s) 532, 659, 720, 1127, 1529
Hpy8I GTNNAC 5 cut(s) 532, 659, 720, 1127, 1529
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 9 cut(s) 118, 839, 934, 1316, 1453, 1884, 1927, 2120, 2779
HpyCH4III ACNGT 8 cut(s) 328, 492, 957, 1564, 2188, 2243, 2705, 2854
HpyCH4IV ACGT 5 cut(s) 39, 534, 596, 1821, 2523
HpySE526I ACGT 5 cut(s) 39, 534, 596, 1821, 2523
Hsp92I GRCGYC 1 cut(s) 596
HspAI GCGC 4 cut(s) 236, 502, 1131, 1789
Kpn2I TCCGGA 3 cut(s) 520, 1783, 2680
LguI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
LmnI GCTCC 9 cut(s) 88, 753, 1279, 1786, 2083, 2087, 2191, 2789, 3017
Lsp1109I GCAGC 3 cut(s) 1237, 1405, 2963
MaeII ACGT 5 cut(s) 39, 534, 596, 1821, 2523
MaeIII GTNAC 7 cut(s) 271, 951, 1045, 1372, 1490, 2048, 2713
MfeI CAATTG 1 cut(s) 2582
MflI RGATCY 8 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2866, 2930
MhlI GDGCHC 8 cut(s) 93, 772, 851, 999, 1864, 1921, 2437, 2941
MlsI TGGCCA 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 3 cut(s) 616, 1564, 1976
MmeI TCCRAC 3 cut(s) 329, 367, 809
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 927
MroI TCCGGA 3 cut(s) 520, 1783, 2680
MroXI GAANNNNTTC 1 cut(s) 2130
MscI TGGCCA 1 cut(s) 207
MslI CAYNNNNRTG 2 cut(s) 930, 1995
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 2828, 2996
MspCI CTTAAG 1 cut(s) 2389
MspI CCGG 6 cut(s) 6, 521, 1631, 1784, 2549, 2681
MspR9I CCNGG 9 cut(s) 701, 752, 917, 1512, 1631, 2085, 2549, 2727, 3015
MunI CAATTG 1 cut(s) 2582
Mva1269I GAATGC 1 cut(s) 1041
MvaI CCWGG 7 cut(s) 701, 752, 917, 1512, 2085, 2727, 3015
MvnI CGCG 2 cut(s) 1033, 1131
NciI CCSGG 2 cut(s) 1631, 2549
NcoI CCATGG 2 cut(s) 1452, 2305
NdeI CATATG 4 cut(s) 180, 210, 1600, 2098
NmuCI GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
NsiI ATGCAT 1 cut(s) 927
NspI RCATGY 1 cut(s) 111
PaeR7I CTCGAG 2 cut(s) 2430, 2934
PagI TCATGA 1 cut(s) 637
PasI CCCWGGG 1 cut(s) 2726
PciSI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
PcsI WCGNNNNNNNCGW 1 cut(s) 602
PctI GAATGC 1 cut(s) 1041
PdmI GAANNNNTTC 1 cut(s) 2130
PfeI GAWTC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 3013
PkrI GCNGC 6 cut(s) 487, 1252, 1395, 1405, 2953, 2998
PleI GAGTC 3 cut(s) 615, 1564, 1975
PpsI GAGTC 3 cut(s) 615, 1564, 1975
PpuMI RGGWCCY 1 cut(s) 826
PshBI ATTAAT 1 cut(s) 2912
Psp124BI GAGCTC 2 cut(s) 93, 2941
Psp1406I AACGTT 2 cut(s) 534, 1821
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 7 cut(s) 699, 750, 915, 1510, 2083, 2725, 3013
PspEI GGTNACC 1 cut(s) 1490
PspFI CCCAGC 4 cut(s) 543, 1325, 2824, 2992
PspGI CCWGG 7 cut(s) 699, 750, 915, 1510, 2083, 2725, 3013
PspPI GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
PspPPI RGGWCCY 1 cut(s) 826
PspXI VCTCGAGB 1 cut(s) 2430
PstI CTGCAG 1 cut(s) 1395
PstNI CAGNNNCTG 1 cut(s) 2414
PsuI RGATCY 8 cut(s) 580, 796, 1633, 2017, 2260, 2398, 2866, 2930
RsaI GTAC 8 cut(s) 106, 647, 719, 805, 2163, 2412, 2442, 2518
RsaNI GTAC 8 cut(s) 105, 646, 718, 804, 2162, 2411, 2441, 2517
RseI CAYNNNNRTG 2 cut(s) 930, 1995
SacI GAGCTC 2 cut(s) 93, 2941
SapI GCTCTTC 4 cut(s) 777, 972, 2166, 2682
SatI GCNGC 6 cut(s) 486, 1251, 1394, 1404, 2952, 2997
Sau96I GGNCC 7 cut(s) 826, 1742, 1960, 2060, 2111, 2627, 2957
SchI GAGTC 3 cut(s) 616, 1564, 1976
ScrFI CCNGG 9 cut(s) 701, 752, 917, 1512, 1631, 2085, 2549, 2727, 3015
SduI GDGCHC 8 cut(s) 93, 772, 851, 999, 1864, 1921, 2437, 2941
SfcI CTRYAG 3 cut(s) 1391, 1560, 2784
Sfr274I CTCGAG 2 cut(s) 2430, 2934
SinI GGWCC 5 cut(s) 826, 1960, 2060, 2111, 2957
SlaI CTCGAG 2 cut(s) 2430, 2934
SmiMI CAYNNNNRTG 2 cut(s) 930, 1995
SmlI CTYRAG 4 cut(s) 649, 2389, 2430, 2934
SmoI CTYRAG 4 cut(s) 649, 2389, 2430, 2934
SspI AATATT 6 cut(s) 394, 678, 779, 840, 1721, 2206
SstI GAGCTC 2 cut(s) 93, 2941
StyD4I CCNGG 9 cut(s) 699, 750, 915, 1510, 1629, 2083, 2547, 2725, 3013
StyI CCWWGG 4 cut(s) 381, 743, 1452, 2305
TaaI ACNGT 8 cut(s) 328, 492, 957, 1564, 2188, 2243, 2705, 2854
TaiI ACGT 5 cut(s) 42, 537, 599, 1824, 2526
TaqI TCGA 6 cut(s) 1317, 1568, 1689, 1978, 2431, 2935
TaqII GACCGA 1 cut(s) 2048
TatI WGTACW 1 cut(s) 717
TauI GCSGC 3 cut(s) 488, 1406, 2999
TfiI GAWTC 1 cut(s) 416
TseFI GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
TseI GCWGC 3 cut(s) 1250, 1393, 2951
Tsp45I GTSAC 5 cut(s) 951, 1045, 1490, 2048, 2713
TspGWI ACGGA 3 cut(s) 13, 980, 1973
Vha464I CTTAAG 1 cut(s) 2389
VpaK11BI GGWCC 5 cut(s) 826, 1960, 2060, 2111, 2957
VspI ATTAAT 1 cut(s) 2912
XapI RAATTY 3 cut(s) 1217, 1778, 2894
XceI RCATGY 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 2800
XhoI CTCGAG 2 cut(s) 2430, 2934
XmiI GTMKAC 2 cut(s) 658, 1126
XmnI GAANNNNTTC 1 cut(s) 2130
ZraI GACGTC 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.