Rroxscaffold_1G00024580

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007314
Physical Location & Seq
Forward (+)
30529665 .. 30541669
12005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024580.1

Sequence Viewer

Length: 3171 bp
ATGGCTTTGTCCGGCATGCGAGGTCTCTCTGTTTTCATCAGCGACATTCGGAATTGCCAGAACAAAGAGCAGGAGAGGCTCCGTGTCGATAAGGAGCTCGGGAATGTTCGTACGCGCTTCAAAAACGAAAAGGGTTTGACACCGTATGAGAAGAAGAAATATGTCTGGAAAATGCTTTACATTTATATGTTGGGATATGATGTGGATTTTGGTCACATGGAAGCAGTCTCTCTTATATCAGCGCCCAAGTATCCAGAAAAGCAGGTTGGGTACATTGTGACATCATGTCTACTCAATGAAAACCACGATTTTCTGAGATTAGCGATCAATACTGTACGGAATGATATTATTGGTCGTAATGAAACTTTCCAATGTTTGGCATTGACTATGGTTGGGAATATTGGAGGCAGAGAATTTGCGGAGTCATTAGCACCTGATGTTCAAAAGTTGCTTATTTCTAGCAGCTACAGACCGCTTGTGAGAAAAAAAGCTGCACTATGTCTTTTGCGCTTGTATAGGAAAAATCCTGATGTTGTCAATATAGATGGCTGGGCCGATCGCATGGCACAACTTTTAGATGAACGTGATCTTGGTGTTTTGACATCATCTATGAGCCTTCTTGTTGCGCTTGTATCAAATCACCATGATGCTTACTGGAGTTGTCTTCCAAAATGTGTTAAAATACTAGAACGGCTTGCGAGGAACCAGGATATTCCACAAGAGTACACCTACTATGGCATCCCATCTCCCTGGCTCCAGGTCAAGACAATGAGGGTTCTGCAGTATTTCCCCACTGTTGAAGATCCAAATACAAGAAGATCATTGTTTGAGGTCTTACAACGTATATTGATGGGTACTGATGTTGTGAAAAATGTTAATAAGAACAACGCATCGCACGCTGTTCTTTTTGAAGCCCTCGCACTTGTCATGCATCTTGATGCTGAGAAGGAGATGATGTCCCAATGTGTTGCACTTCTGGGAAAATTTATTGCTGTGCGAGAACCAAATATTCGATATCTTGGTTTGGAAAATATGACCCGCATGCTAATGGTAACGGATGTGCAAGATATCATCAAGAGACATCAAGCCCAGATTATCACCTCATTGAAGGATCCAGATGTCAGCATTCGTAGGCGTGCTCTTGACCTGCTTTATGGCATGTGTGATGTTTCAAATGCAAAAGACATAGTTGAAGAATTATTGCAGTATCTTAGTACAGCAGACTTCGCAATGCGCGAGGAATTGTCACTTAAGGCTGCCATTCTTGCAGAAAAATTTGCTCCTGATCTATCATGGTATGTAGATGTGATTCTTCAATTGATTGATAAGGCAGGAGATTTTGTTAGTGATGATATATGGTTCCGTGTTGTGCAATTTGTTACGAATAATGAAGACTTACAGCCTTATGCTGCAGCAAAAGCCAGGGAGTATCTTGACAAACCTGCTATACATGAGACAATGGTTAAGGTCAGTGCATATATCATTGGAGAATTTGGTCACCTTTTAGCCAGACGGCCTGGGTGTAGTCCAAAAGAATTATTTGCCGTCATACATGAGAAGCTTCCTACTGTATCCACTTCTACAATACCTATTCTTCTTTCAACATATGCTAAGATCTTTATGCACACTCAACCTCCAGACCAAGAACTACAGAATCAGATTTGGGCCATATTCAATAAATACGAGAGTTGCATCGATGTTGAGATACAACAACGAGCTGCTGAATACCTTGCCTTAAGTAGGAGAGGTGAAGCTCTTGTTGATATATTGGCTGAAATGCCTAAATTCCCTGAGCGGCAGTCTGCATTGATCAAAAAGGCTGAAGATACTGAAGTTGATACCGCAGAACAAAGTGCTATCAAGTTGCGCACCCAGCAGCAAACATCAAATGCTTTGGTTGTGACCGACCAACGTCCCGCTAATGGTACCCCACCTGCCAACCACCAGCTTGGTCTTGTGAAGATACCCAACATGAGCAGTAATGTGGATCACAATTCAACCGATCAAGGGTTGTCTCAGGAAAATGGTACTTTGAATAAAGTTGATCCTCAAACTCCTTCACCGGATCTCCTTGGTGACCTCTTGGGTCCGCTGGCAATTGAAGGCCCTCCGGGTACTGCTGTTCAATCACATCAAACTGCAATCCCTGGATCTGGTGGTGATCCAAATGCAGTGGATGCTACGGCCATAGTTCCTGTTGGCGATGAGCCAAATTCTGTTCAGCCTATAGGAAATATTGCAGAAAGATTTCTTGCCTTGTGCTTGAAAGATAGTGGTGTTCTGTATGAAGATCCTAATATTCAGATCGGAATTAAAGCAGAGTGGCGCTTACATCAGGGTTGTCTTGTTCTTTTCTTGGGAAACAAAAATACTGCACCACTTGTATCAGTTCAGGCTGTAATAATGCCTCCCTCCCACTTTAAAATGGAGCTCTCACTAGTGCCAGAGACTATTCCTCCTAGGGCACAGGTACAGTGCCCCCTTGAAGTTGTGAATCTCCGTCCAAGTAGGGATGTAGCAGTTCTCGACTTCTCTTACAAGTTTGGTCACAATATGGTCAATGTCAAGCTTCGTCTCCCTGCTGTCTTGAATAAATTTCTTCAACCTATACCAGTGTCTGCTGAAGAGTTCTTTCCTCAGTGGAGATCACTTTCAGGACCACCTCTGAAGCTTCAAGAAGTGGTTAGAGGCGTCAAACCACTGCCACTTGCAGAAATGGCAAATCTAATTAACAGCTTCCGGTTGATGGTTTGTCCAGGACTTGACCCTAATCCAAATAATCTTGTTGCAAGCACAACCTTTTATTCAGAAAGTACACGGGCGATGCTTTGCTTGGCTAGAATAGAAACAGATCCAGCAGACAGAACCCAGTTGCGTATGACGGTTGCTTCAGGGGATCCTACGTTAACATTTGAGTTGAAGGAGTTCATCAAGGAACAAATAGTAAACATCCCTGTAGCTCCTCGTGCTCCTGGACCAGTGCCACCAGCACCTCCAGTAGCTCAACCGACCAGTCCAGCTGCAGCATTAACGGATCCTGGTGCTCTGCTGGCCGGGTCTCGCGCGCAAGTTAAAAATGTGGATAACAAAAATCCTGATGCAAGGGCTGTCGGAATCAAGGATGAGCTAACTTGTGTGAACGGCTTGGTGTTGTGGATCTGCACAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1056

Amino Acids

117.46

Weight (kDa)

6.03

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 28 - 582 2.1e-116 Adaptin N terminal region
Cnd1 PF12717 125 - 263 1.1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 758 - 854 6.3e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 866 - 975 1e-15 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1942
Acc16I TGCGCA 1 cut(s) 1868
Acc36I ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Acc65I GGTACC 1 cut(s) 1925
AccB1I GGYRCC 1 cut(s) 1925
AccB7I CCANNNNNTGG 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 1795
AccI GTMKAC 1 cut(s) 289
AccII CGCG 4 cut(s) 115, 1236, 3063, 3065
AciI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1917, 2090
AcoI YGGCCR 2 cut(s) 2184, 3051
AcsI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
AcuI CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
AcyI GRCGYC 1 cut(s) 2691
AfiI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
AflII CTTAAG 2 cut(s) 1250, 1735
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 2437
AjnI CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 573, 605
Alw21I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
Alw26I GTCTC 8 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579, 3063
AlwNI CAGNNNCTG 1 cut(s) 2618
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 6 cut(s) 552, 1514, 1665, 2104, 2184, 3051
ApeKI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
ApoI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
Asp700I GAANNNNTTC 2 cut(s) 2247, 2924
Asp718I GGTACC 1 cut(s) 1925
AspA2I CCTAGG 1 cut(s) 2459
AspLEI GCGC 9 cut(s) 117, 244, 510, 628, 1236, 1869, 2328, 3065, 3067
AspS9I GGNCC 6 cut(s) 552, 1665, 2087, 2105, 2657, 2975
AsuC2I CCSGG 2 cut(s) 2112, 3055
AsuHPI GGTGA 7 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2171
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 3 cut(s) 2087, 2657, 2975
AvrII CCTAGG 1 cut(s) 2459
BaeGI GKGCMC 2 cut(s) 2467, 2480
BaeI ACNNNNGTAYC 6 cut(s) 846, 879, 1818, 1851, 2106, 2139
BamHI GGATCC 3 cut(s) 1111, 2896, 3034
BanI GGYRCC 1 cut(s) 1925
BanII GRGCYC 2 cut(s) 99, 2433
BauI CACGAG 1 cut(s) 2964
BbsI GAAGAC 2 cut(s) 656, 1398
Bbv12I GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BbvI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BccI CCATC 4 cut(s) 539, 751, 844, 2740
BceAI ACGGC 5 cut(s) 707, 1529, 1529, 2199, 3157
BciT130I CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BciVI GTATCC 2 cut(s) 261, 1582
BclI TGATCA 1 cut(s) 1809
BcnI CCSGG 2 cut(s) 2112, 3055
BcoDI GTCTC 8 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579, 3063
BcuI ACTAGT 1 cut(s) 2437
BfaI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
BfmI CTRYAG 7 cut(s) 466, 779, 1410, 1649, 2226, 2955, 3021
BfoI RGCGCY 2 cut(s) 245, 2329
BfrI CTTAAG 2 cut(s) 1250, 1735
BfuAI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BfuI GTATCC 2 cut(s) 261, 1582
BglII AGATCT 1 cut(s) 1614
BlnI CCTAGG 1 cut(s) 2459
Bme18I GGWCC 3 cut(s) 2087, 2657, 2975
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 6 cut(s) 552, 1665, 2087, 2105, 2657, 2975
BmiI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BmrI ACTGGG 1 cut(s) 2863
BmsI GCATC 9 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814, 3088
BmuI ACTGGG 1 cut(s) 2863
BoxI GACNNNNGTC 1 cut(s) 1911
BpiI GAAGAC 2 cut(s) 656, 1398
BpmI CTGGAG 4 cut(s) 676, 740, 1620, 2979
Bpu10I CCTNAGC 1 cut(s) 1791
BpuMI CCSGG 2 cut(s) 2112, 3055
Bsa29I ATCGAT 1 cut(s) 1695
BsaHI GRCGYC 1 cut(s) 2691
BsaI GGTCTC 2 cut(s) 29, 3063
BsaJI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BsaWI WCCGGW 2 cut(s) 2062, 2739
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
Bse1I ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
Bse3DI GCAATG 1 cut(s) 1236
BseBI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BseCI ATCGAT 1 cut(s) 1695
BseDI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BseGI GGATG 6 cut(s) 738, 1063, 2182, 2518, 2949, 3127
BseLI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 5 cut(s) 305, 933, 1782, 2030, 2651
BseNI ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
BsePI GCGCGC 1 cut(s) 3063
BseRI GAGGAG 1 cut(s) 2952
BseSI GKGCMC 2 cut(s) 2467, 2480
BseXI GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BseYI CCCAGC 2 cut(s) 549, 1872
BsgI GTGCAG 3 cut(s) 477, 2358, 3145
Bsh1236I CGCG 4 cut(s) 115, 1236, 3063, 3065
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 6 cut(s) 554, 1516, 1667, 2106, 2186, 3053
BshNI GGYRCC 1 cut(s) 1925
BshVI ATCGAT 1 cut(s) 1695
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 5 cut(s) 99, 1141, 2433, 2971, 3046
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
BsiWI CGTACG 1 cut(s) 110
BslFI GGGAC 2 cut(s) 943, 1899
BslI CCNNNNNNNGG 6 cut(s) 376, 1740, 1922, 2007, 2153, 2746
BsmAI GTCTC 8 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579, 3063
BsmBI CGTCTC 1 cut(s) 2579
BsmFI GGGAC 2 cut(s) 943, 1899
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 6 cut(s) 554, 1516, 1667, 2106, 2186, 3053
Bso31I GGTCTC 2 cut(s) 29, 3063
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
BspACI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1917, 2090
BspANI GGCC 6 cut(s) 554, 1516, 1667, 2106, 2186, 3053
BspCNI CTCAG 5 cut(s) 306, 934, 1783, 2029, 2650
BspDI ATCGAT 1 cut(s) 1695
BspFNI CGCG 4 cut(s) 115, 1236, 3063, 3065
BspLI GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
BspMAI CTGCAG 3 cut(s) 783, 1414, 3025
BspMI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
BspT107I GGYRCC 1 cut(s) 1925
BspTI CTTAAG 2 cut(s) 1250, 1735
BspTNI GGTCTC 2 cut(s) 29, 3063
BsrBI CCGCTC 1 cut(s) 1795
BsrDI GCAATG 1 cut(s) 1236
BsrI ACTGG 6 cut(s) 659, 2612, 2869, 2978, 2996, 3012
BssECI CCNNGG 6 cut(s) 749, 1422, 1517, 2071, 2146, 2459
BssHII GCGCGC 1 cut(s) 3063
BssNI GRCGYC 1 cut(s) 2691
BssSI CACGAG 1 cut(s) 2964
BssT1I CCWWGG 2 cut(s) 2071, 2459
Bst2BI CACGAG 1 cut(s) 2964
Bst2UI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
Bst4CI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
Bst6I CTCTTC 1 cut(s) 2619
BstACI GRCGYC 1 cut(s) 2691
BstAFI CTTAAG 2 cut(s) 1250, 1735
BstAPI GCANNNNNTGC 1 cut(s) 2177
BstC8I GCNNGC 9 cut(s) 17, 696, 897, 1043, 1137, 2094, 2791, 3051, 3065
BstDEI CTNAG 7 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637
BstEII GGTNACC 2 cut(s) 1496, 2075
BstENI CCTNNNNNAGG 1 cut(s) 1738
BstF5I GGATG 6 cut(s) 738, 1063, 2182, 2518, 2949, 3127
BstFNI CGCG 4 cut(s) 115, 1236, 3063, 3065
BstH2I RGCGCY 2 cut(s) 245, 2329
BstHHI GCGC 9 cut(s) 117, 244, 510, 628, 1236, 1869, 2328, 3065, 3067
BstMAI GTCTC 8 cut(s) 29, 232, 1072, 1448, 2019, 2441, 2579, 3063
BstMCI CGRYCG 1 cut(s) 559
BstNI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
BstNSI RCATGY 3 cut(s) 19, 1045, 1162
BstPAI GACNNNNGTC 1 cut(s) 1911
BstPI GGTNACC 2 cut(s) 1496, 2075
BstSFI CTRYAG 7 cut(s) 466, 779, 1410, 1649, 2226, 2955, 3021
BstSLI GKGCMC 2 cut(s) 2467, 2480
BstUI CGCG 4 cut(s) 115, 1236, 3063, 3065
BstV1I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
BstV2I GAAGAC 2 cut(s) 656, 1398
BstXI CCANNNNNNTGG 2 cut(s) 750, 1949
Bsu15I ATCGAT 1 cut(s) 1695
BsuI GTATCC 2 cut(s) 261, 1582
BsuRI GGCC 6 cut(s) 554, 1516, 1667, 2106, 2186, 3053
BsuTUI ATCGAT 1 cut(s) 1695
BtgZI GCGATG 3 cut(s) 876, 2217, 2837
BtsCI GGATG 6 cut(s) 738, 1063, 2182, 2518, 2949, 3127
BtsI GCAGTG 2 cut(s) 2178, 2699
BtsIMutI CAGTG 8 cut(s) 792, 1477, 2178, 2480, 2619, 2645, 2699, 2985
BveI ACCTGC 4 cut(s) 253, 1155, 1450, 1942
Cac8I GCNNGC 9 cut(s) 17, 696, 897, 1043, 1137, 2094, 2791, 3051, 3065
CaiI CAGNNNCTG 1 cut(s) 2618
CfoI GCGC 9 cut(s) 117, 244, 510, 628, 1236, 1869, 2328, 3065, 3067
Cfr13I GGNCC 6 cut(s) 552, 1665, 2087, 2105, 2657, 2975
ClaI ATCGAT 1 cut(s) 1695
CseI GACGC 1 cut(s) 2680
CspCI CAANNNNNGTGG 2 cut(s) 2154, 2189
DdeI CTNAG 7 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637
DraI TTTAAA 1 cut(s) 2422
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 2 cut(s) 2184, 3051
Eam1104I CTCTTC 1 cut(s) 2619
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 2619
Ecl136II GAGCTC 2 cut(s) 97, 2431
Eco130I CCWWGG 2 cut(s) 2071, 2459
Eco24I GRGCYC 2 cut(s) 99, 2433
Eco31I GGTCTC 2 cut(s) 29, 3063
Eco32I GATATC 2 cut(s) 1016, 1069
Eco47I GGWCC 3 cut(s) 2087, 2657, 2975
Eco53kI GAGCTC 2 cut(s) 97, 2431
Eco57I CTGAAG 5 cut(s) 1842, 1851, 2643, 2687, 2874
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 2 cut(s) 1496, 2075
EcoICRI GAGCTC 2 cut(s) 97, 2431
EcoNI CCTNNNNNAGG 1 cut(s) 1738
EcoO109I RGGNCCY 1 cut(s) 2105
EcoO65I GGTNACC 2 cut(s) 1496, 2075
EcoRII CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
EcoRV GATATC 2 cut(s) 1016, 1069
EcoT14I CCWWGG 2 cut(s) 2071, 2459
EcoT22I ATGCAT 1 cut(s) 933
EcoT38I GRGCYC 2 cut(s) 99, 2433
ErhI CCWWGG 2 cut(s) 2071, 2459
Esp3I CGTCTC 1 cut(s) 2579
FaqI GGGAC 2 cut(s) 943, 1899
FauI CCCGC 2 cut(s) 1046, 1924
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 1809
FblI GTMKAC 1 cut(s) 289
FokI GGATG 6 cut(s) 725, 1070, 2189, 2525, 2936, 3134
FriOI GRGCYC 2 cut(s) 99, 2433
FspBI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
FspI TGCGCA 1 cut(s) 1868
GlaI GCGC 9 cut(s) 116, 243, 509, 627, 1235, 1868, 2327, 3064, 3066
GsaI CCCAGC 2 cut(s) 553, 1876
GsuI CTGGAG 4 cut(s) 676, 740, 1620, 2979
HaeII RGCGCY 2 cut(s) 245, 2329
HaeIII GGCC 6 cut(s) 554, 1516, 1667, 2106, 2186, 3053
HapII CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
HgaI GACGC 1 cut(s) 2680
HhaI GCGC 9 cut(s) 117, 244, 510, 628, 1236, 1869, 2328, 3065, 3067
Hin1I GRCGYC 1 cut(s) 2691
Hin6I GCGC 9 cut(s) 115, 242, 508, 626, 1234, 1867, 2326, 3063, 3065
HinP1I GCGC 9 cut(s) 115, 242, 508, 626, 1234, 1867, 2326, 3063, 3065
HincII GTYRAC 1 cut(s) 2907
HindII GTYRAC 1 cut(s) 2907
HindIII AAGCTT 3 cut(s) 1559, 2567, 2669
HinfI GANTC 5 cut(s) 422, 1309, 1654, 2494, 3114
HpaI GTTAAC 1 cut(s) 2907
HpaII CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
HphI GGTGA 7 cut(s) 632, 1090, 1490, 1760, 2052, 2087, 2171
Hpy166II GTNNAC 6 cut(s) 290, 726, 2816, 2907, 2947, 3139
Hpy188I TCNGA 8 cut(s) 51, 315, 1659, 2304, 2309, 2667, 2809, 3113
Hpy8I GTNNAC 6 cut(s) 290, 726, 2816, 2907, 2947, 3139
HpyAV CCTTC 6 cut(s) 626, 940, 1102, 2067, 2096, 2914
HpyCH4III ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
HpyCH4IV ACGT 4 cut(s) 583, 841, 1912, 2903
HpyF3I CTNAG 7 cut(s) 314, 942, 1211, 1611, 1791, 2016, 2637
HpySE526I ACGT 4 cut(s) 583, 841, 1912, 2903
Hsp92I GRCGYC 1 cut(s) 2691
HspAI GCGC 9 cut(s) 115, 242, 508, 626, 1234, 1867, 2326, 3063, 3065
KpnI GGTACC 1 cut(s) 1929
Ksp22I TGATCA 1 cut(s) 1809
KspAI GTTAAC 1 cut(s) 2907
LmnI GCTCC 7 cut(s) 84, 94, 759, 1285, 2428, 2965, 2974
Lsp1109I GCAGC 9 cut(s) 474, 478, 1243, 1396, 1424, 1705, 1888, 3007, 3035
LweI GCATC 9 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814, 3088
MaeI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
MaeII ACGT 4 cut(s) 583, 841, 1912, 2903
MaeIII GTNAC 9 cut(s) 212, 277, 1051, 1245, 1378, 1496, 1900, 2075, 2546
MbiI CCGCTC 1 cut(s) 1795
MfeI CAATTG 2 cut(s) 1316, 2097
MhlI GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 3091
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 2 cut(s) 2247, 2924
MslI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
MspA1I CMGCKG 2 cut(s) 2092, 3020
MspCI CTTAAG 2 cut(s) 1250, 1735
MspI CCGG 5 cut(s) 12, 2063, 2111, 2740, 3054
MunI CAATTG 2 cut(s) 1316, 2097
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 9 cut(s) 707, 751, 758, 1423, 1518, 2148, 2757, 2973, 3039
MvnI CGCG 4 cut(s) 115, 1236, 3063, 3065
NciI CCSGG 2 cut(s) 2112, 3055
NdeI CATATG 1 cut(s) 1606
NlaIV GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
NmuCI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
NsbI TGCGCA 1 cut(s) 1868
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 3 cut(s) 19, 1045, 1162
PaeI GCATGC 2 cut(s) 19, 1045
PaqCI CACCTGC 1 cut(s) 1942
PauI GCGCGC 1 cut(s) 3063
PcsI WCGNNNNNNNCGW 1 cut(s) 1233
PctI GAATGC 1 cut(s) 1125
PdmI GAANNNNTTC 2 cut(s) 2247, 2924
PfeI GAWTC 4 cut(s) 1309, 1654, 2494, 3114
Pfl23II CGTACG 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 376
PfoI TCCNGGA 2 cut(s) 2755, 2971
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1911
Psp124BI GAGCTC 2 cut(s) 99, 2433
Psp6I CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
PspEI GGTNACC 2 cut(s) 1496, 2075
PspFI CCCAGC 2 cut(s) 549, 1872
PspGI CCWGG 9 cut(s) 705, 749, 756, 1421, 1516, 2146, 2755, 2971, 3037
PspLI CGTACG 1 cut(s) 110
PspN4I GGNNCC 9 cut(s) 80, 704, 755, 1113, 1361, 1927, 2088, 2898, 3036
PspPI GGNCC 6 cut(s) 552, 1665, 2087, 2105, 2657, 2975
PstI CTGCAG 3 cut(s) 783, 1414, 3025
PstNI CAGNNNCTG 1 cut(s) 2618
PteI GCGCGC 1 cut(s) 3063
PvuI CGATCG 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 3020
RseI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SacI GAGCTC 2 cut(s) 99, 2433
Sau96I GGNCC 6 cut(s) 552, 1665, 2087, 2105, 2657, 2975
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 7 cut(s) 99, 1141, 2433, 2467, 2480, 2971, 3046
SfaNI GCATC 9 cut(s) 637, 747, 899, 928, 940, 1701, 2167, 2814, 3088
SfcI CTRYAG 7 cut(s) 466, 779, 1410, 1649, 2226, 2955, 3021
SinI GGWCC 3 cut(s) 2087, 2657, 2975
SmiMI CAYNNNNRTG 4 cut(s) 185, 645, 936, 1046
SmlI CTYRAG 2 cut(s) 1250, 1735
SmoI CTYRAG 2 cut(s) 1250, 1735
SpeI ACTAGT 1 cut(s) 2437
SphI GCATGC 2 cut(s) 19, 1045
SsiI CCGC 7 cut(s) 419, 473, 1039, 1795, 1842, 1917, 2090
SspI AATATT 4 cut(s) 400, 1009, 2236, 2299
SspMI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
SstI GAGCTC 2 cut(s) 99, 2433
StyI CCWWGG 2 cut(s) 2071, 2459
TaaI ACNGT 6 cut(s) 144, 334, 796, 1570, 2475, 2884
TaiI ACGT 4 cut(s) 586, 844, 1915, 2906
TaqI TCGA 4 cut(s) 87, 1012, 1695, 2526
TaqII GACCGA 1 cut(s) 1919
TatI WGTACW 3 cut(s) 723, 1214, 2813
TauI GCSGC 1 cut(s) 1798
TfiI GAWTC 4 cut(s) 1309, 1654, 2494, 3114
TscAI CASTG 8 cut(s) 799, 1477, 2178, 2480, 2619, 2645, 2706, 2985
TseFI GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TseI GCWGC 9 cut(s) 462, 491, 1256, 1409, 1412, 1718, 1876, 3020, 3023
Tsp45I GTSAC 7 cut(s) 212, 277, 1245, 1496, 1900, 2075, 2546
TspDTI ATGAA 7 cut(s) 25, 312, 375, 594, 1404, 2301, 2917
TspGWI ACGGA 6 cut(s) 71, 352, 1070, 1352, 2489, 3047
TspRI CASTG 8 cut(s) 799, 1477, 2178, 2480, 2619, 2645, 2706, 2985
Van91I CCANNNNNTGG 1 cut(s) 376
Vha464I CTTAAG 2 cut(s) 1250, 1735
VpaK11BI GGWCC 3 cut(s) 2087, 2657, 2975
XagI CCTNNNNNAGG 1 cut(s) 1738
XapI RAATTY 7 cut(s) 413, 983, 1274, 1490, 1784, 2212, 2594
XceI RCATGY 3 cut(s) 19, 1045, 1162
XmaJI CCTAGG 1 cut(s) 2459
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 2247, 2924
XspI CTAG 5 cut(s) 459, 686, 2438, 2460, 2838
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.