AT5G24830

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009978
Physical Location & Seq
Forward (+)
8530915 .. 8533867
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24830.1

Sequence Viewer

Length: 1782 bp
ATGGCTCTTTTAATATCTTGTGGGGAAGTTACCTCTTCTCAATTCACTGTATTTCGCTTACTCAACCAAAGCCTTGATTTTGTTAGTGACAATGTCTCTAGGCTCTTAGCTCCTATTTTCACCAACCTCAGGGACTTTGAAATGCGTCTTTCTTGTATCGAACGTCCTCCATCCATTTCTGGCAATCATTCTCATTTGTGCACTGAGAAATGGTTTTCTGATCAAAAAGATTATGATCAAAAGGAAGACCCGGAAGCTATTTTCAATGTGTTGGATTATATCTTGAAAAGCAGCTTGGATCGTCTGGCTTCATTGAGGGAGAGTGTATGTCAGACAAAGAGTTTTGATTACGATGATTGTTTATCTATTCACTCGTCTATAATGAGGGATCTTTGTTTGCAAGGGAAGTTGGATGCTGCTCTCTGGCTACGGAAAAAAATGATATATAGTGGAGTTATTCCAGGTTTGATCACGCACAATCATCTTTTAAATGGGTTGTGTAAAGCAGGTTATATCGAGAAGGCGGATGGGCTTGTTAGAGAGATGAGGGAGATGGGTCCTTCGCCTAATTGTGTCTCTTATAACACCTTAATCAAGGGTCTTTGCAGTGTTAACAATGTAGATAAAGCTCTGTACCTCTTCAATACTATGAATAAATATGGTATTAGGCCAAATAGAGTGACTTGCAATATAATTGTGCATGCACTTTGCCAGAAAGGTGTTATAGGAAACAACAACAAAAAGCTTCTTGAAGAGATCTTAGATAGTAGCCAGGCGAACGCACCCTTGGATATAGTCATCTGTACCATTCTGATGGATAGTTGTTTTAAGAATGGAAATGTGGTTCAAGCCCTTGAGGTTTGGAAGGAGATGTCACAGAAAAATGTCCCTGCTGATTCAGTTGTGTACAATGTCATTATTCGTGGGTTATGTTCGAGTGGAAACATGGTAGCTGCTTATGGGTTCATGTGTGACATGGTAAAGAGAGGAGTGAATCCTGATGTTTTCACTTACAACACTCTCATTAGCGCCTTGTGCAAAGAGGGAAAGTTTGATGAGGCATGTGATCTCCATGGTACCATGCAAAATGGTGGTGTTGCTCCTGATCAGATCTCATACAAAGTTATTATTCAAGGTTTATGTATACATGGAGATGTGAACAGGGCGAACGAGTTCCTTCTAAGCATGCTAAAAAGTTCTCTGCTTCCAGAGGTTCTGCTATGGAATGTGGTTATTGATGGTTATGGAAGATATGGTGATACTAGCAGTGCTTTATCTGTTCTGAATCTAATGCTTTCGTACGGTGTTAAACCAAATGTTTACACAAACAATGCTTTGATTCATGGGTATGTGAAAGGGGGAAGATTAATTGATGCATGGTGGGTCAAAAATGAAATGCGATCTACCAAAATCCACCCAGATACTACCACCTATAATCTGCTACTAGGTGCTGCTTGTACATTGGGTCACTTGCGGTTGGCATTTCAGTTGTATGATGAAATGCTGAGAAGGGGATGTCAACCTGATATCATTACGTATACTGAGCTGGTTAGAGGGCTCTGTTGGAAGGGTCGATTGAAGAAAGCCGAAAGCCTTCTGTCGAGAATACAAGCAACTGGTATAACAATAGATCATGTTCCATTTTTGATACTAGCAAAGAAATATACCAGATTGCAGAGGCCAGGCGAGGCGTATTTAGTTTACAAAAAGTGGTTAGCGACGAGGAACAGAGGAGTTTCTTGTCCAAGCATACTAAATCACATGCATACAGAAGAGCAGTAA

Protein Analysis

593

Amino Acids

66.54

Weight (kDa)

8.33

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 151 - 183 5.7e-07 PPR repeat
PPR_2 PF13041 154 - 202 4.5e-14 PPR repeat family
PPR PF01535 157 - 186 3.4e-06 PPR repeat
PPR_3 PF13812 178 - 235 2.9e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 180 - 291 3.9e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 185 - 217 2e-11 PPR repeat
PPR_2 PF13041 189 - 238 2.7e-16 PPR repeat family
PPR PF01535 192 - 222 1.6e-06 PPR repeat
PPR_2 PF13041 264 - 311 6.9e-13 PPR repeat family
TPR_24 PF23276 268 - 389 4e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 272 - 392 1.4e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 299 - 347 5.4e-18 PPR repeat family
PPR_3 PF13812 325 - 378 8.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 329 - 361 3.4e-13 PPR repeat
PPR_2 PF13041 333 - 381 4.8e-17 PPR repeat family
PPR PF01535 336 - 366 5.6e-08 PPR repeat
PPR_2 PF13041 403 - 442 4.3e-08 PPR repeat family
TPR_24 PF23276 408 - 500 3.1e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 428 - 484 5.9e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 440 - 486 1.2e-09 PPR repeat family
PPR_2 PF13041 473 - 521 2.3e-16 PPR repeat family
PPR_1 PF12854 504 - 535 4.4e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
Acc36I ACCTGC 1 cut(s) 497
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 1076
AccI GTMKAC 2 cut(s) 1144, 1538
AciI CCGC 2 cut(s) 524, 1474
AclWI GGATC 2 cut(s) 306, 396
AfaI GTAC 6 cut(s) 635, 805, 908, 1078, 1301, 1459
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 8 cut(s) 140, 265, 286, 643, 752, 848, 1133, 1579
AjnI CCWGG 3 cut(s) 460, 771, 1681
AjuI GAANNNNNNNTTGG 4 cut(s) 278, 310, 770, 802
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
AluBI AGCT 7 cut(s) 110, 257, 294, 629, 745, 953, 1546
AluI AGCT 7 cut(s) 110, 257, 294, 629, 745, 953, 1546
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 2 cut(s) 100, 580
Alw44I GTGCAC 1 cut(s) 199
AlwI GGATC 2 cut(s) 306, 396
AoxI GGCC 2 cut(s) 668, 1679
ApaLI GTGCAC 1 cut(s) 199
ApeKI GCWGC 4 cut(s) 291, 416, 953, 1451
AseI ATTAAT 1 cut(s) 1367
Asp700I GAANNNNTTC 2 cut(s) 1172, 1593
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 1 cut(s) 1031
AspS9I GGNCC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 2 cut(s) 112, 1268
AvaII GGWCC 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 1560
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 4 cut(s) 303, 403, 940, 1438
BccI CCATC 5 cut(s) 178, 521, 547, 808, 1232
BciT130I CCWGG 3 cut(s) 462, 773, 1683
BclI TGATCA 4 cut(s) 220, 235, 468, 1105
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 100, 580
BfaI CTAG 4 cut(s) 99, 1263, 1445, 1652
BfoI RGCGCY 1 cut(s) 1032
BfuAI ACCTGC 1 cut(s) 497
BglII AGATCT 2 cut(s) 756, 1110
BisI GCNGC 4 cut(s) 292, 417, 954, 1452
BlsI GCNGC 4 cut(s) 293, 418, 955, 1453
Bme1390I CCNGG 4 cut(s) 251, 462, 773, 1683
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 558, 1078
BmrFI CCNGG 4 cut(s) 251, 462, 773, 1683
BmsI GCATC 2 cut(s) 403, 1363
BpiI GAAGAC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 1536
BsaBI GATNNNNATC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 786, 1072
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 1621
Bse21I CCTNAGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 3 cut(s) 462, 773, 1683
BseDI CCNNGG 2 cut(s) 786, 1072
BseGI GGATG 4 cut(s) 170, 418, 532, 1520
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMII CTCAG 4 cut(s) 142, 195, 1496, 1533
BseNI ACTGG 1 cut(s) 1621
BseRI GAGGAG 2 cut(s) 1002, 1746
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 4 cut(s) 303, 403, 940, 1438
BshFI GGCC 2 cut(s) 670, 1681
BshNI GGYRCC 1 cut(s) 1076
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 1 cut(s) 251
BsiWI CGTACG 1 cut(s) 1299
BslFI GGGAC 2 cut(s) 146, 872
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 2 cut(s) 100, 580
BsmFI GGGAC 2 cut(s) 146, 872
BsnI GGCC 2 cut(s) 670, 1681
Bsp1286I GDGCHC 2 cut(s) 203, 1560
Bsp1407I TGTACA 2 cut(s) 906, 1457
Bsp19I CCATGG 1 cut(s) 1072
BspACI CCGC 2 cut(s) 524, 1474
BspANI GGCC 2 cut(s) 670, 1681
BspCNI CTCAG 4 cut(s) 141, 196, 1497, 1534
BspLI GGNNCC 2 cut(s) 558, 1078
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 2 cut(s) 306, 396
BspQI GCTCTTC 1 cut(s) 1767
BspT107I GGYRCC 1 cut(s) 1076
BsrGI TGTACA 2 cut(s) 906, 1457
BsrI ACTGG 1 cut(s) 1621
BssECI CCNNGG 2 cut(s) 786, 1072
BssNAI GTATAC 2 cut(s) 1145, 1539
BssT1I CCWWGG 2 cut(s) 786, 1072
Bst1107I GTATAC 2 cut(s) 1145, 1539
Bst2UI CCWGG 3 cut(s) 462, 773, 1683
Bst4CI ACNGT 2 cut(s) 49, 1304
Bst6I CTCTTC 4 cut(s) 40, 644, 747, 1767
BstAUI TGTACA 2 cut(s) 906, 1457
BstBAI YACGTR 1 cut(s) 1536
BstC8I GCNNGC 2 cut(s) 702, 1187
BstDEI CTNAG 7 cut(s) 106, 128, 204, 760, 1181, 1505, 1542
BstDSI CCRYGG 1 cut(s) 1072
BstF5I GGATG 4 cut(s) 170, 418, 532, 1520
BstH2I RGCGCY 1 cut(s) 1032
BstHHI GCGC 1 cut(s) 1031
BstMAI GTCTC 2 cut(s) 100, 580
BstMWI GCNNNNNNNGC 1 cut(s) 1035
BstNI CCWGG 3 cut(s) 462, 773, 1683
BstNSI RCATGY 4 cut(s) 704, 1065, 1189, 1765
BstSCI CCNGG 4 cut(s) 249, 460, 771, 1681
BstSLI GKGCMC 1 cut(s) 203
BstSNI TACGTA 1 cut(s) 1536
BstV1I GCAGC 4 cut(s) 303, 403, 940, 1438
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 3 cut(s) 388, 756, 1110
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 3 cut(s) 388, 756, 1110
BstZ17I GTATAC 2 cut(s) 1145, 1539
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 2 cut(s) 670, 1681
BtgI CCRYGG 1 cut(s) 1072
BtsCI GGATG 4 cut(s) 170, 418, 532, 1520
BtsI GCAGTG 2 cut(s) 613, 1273
BtsIMutI CAGTG 4 cut(s) 45, 201, 613, 1273
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 702, 1187
CfoI GCGC 1 cut(s) 1031
Cfr13I GGNCC 1 cut(s) 557
CseI GACGC 1 cut(s) 134
Csp6I GTAC 6 cut(s) 634, 804, 907, 1077, 1300, 1458
CviQI GTAC 6 cut(s) 634, 804, 907, 1077, 1300, 1458
DdeI CTNAG 7 cut(s) 106, 128, 204, 760, 1181, 1505, 1542
DraI TTTAAA 1 cut(s) 489
Eam1104I CTCTTC 4 cut(s) 40, 644, 747, 1767
EarI CTCTTC 4 cut(s) 40, 644, 747, 1767
EciI GGCGGA 1 cut(s) 539
Eco105I TACGTA 1 cut(s) 1536
Eco130I CCWWGG 2 cut(s) 786, 1072
Eco24I GRGCYC 1 cut(s) 1560
Eco32I GATATC 1 cut(s) 1528
Eco47I GGWCC 1 cut(s) 557
Eco81I CCTNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 557
EcoRII CCWGG 3 cut(s) 460, 771, 1681
EcoRV GATATC 1 cut(s) 1528
EcoT14I CCWWGG 2 cut(s) 786, 1072
EcoT22I ATGCAT 2 cut(s) 1378, 1767
EcoT38I GRGCYC 1 cut(s) 1560
ErhI CCWWGG 2 cut(s) 786, 1072
FaqI GGGAC 2 cut(s) 146, 872
FbaI TGATCA 4 cut(s) 220, 235, 468, 1105
FblI GTMKAC 2 cut(s) 1144, 1538
Fnu4HI GCNGC 4 cut(s) 292, 417, 954, 1452
FokI GGATG 4 cut(s) 157, 425, 539, 1527
FriOI GRGCYC 1 cut(s) 1560
Fsp4HI GCNGC 4 cut(s) 292, 417, 954, 1452
FspBI CTAG 4 cut(s) 99, 1263, 1445, 1652
GlaI GCGC 1 cut(s) 1030
GluI GCNGC 4 cut(s) 292, 417, 954, 1452
HaeII RGCGCY 1 cut(s) 1032
HaeIII GGCC 2 cut(s) 670, 1681
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 1031
Hin6I GCGC 1 cut(s) 1029
HinP1I GCGC 1 cut(s) 1029
HincII GTYRAC 2 cut(s) 613, 1520
HindII GTYRAC 2 cut(s) 613, 1520
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 4 cut(s) 896, 994, 1285, 1339
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 1 cut(s) 251
HphI GGTGA 2 cut(s) 112, 1268
Hpy166II GTNNAC 9 cut(s) 201, 613, 907, 1145, 1159, 1321, 1520, 1539, 1702
Hpy188I TCNGA 5 cut(s) 220, 333, 813, 1110, 1284
Hpy188III TCNNGA 7 cut(s) 283, 517, 749, 998, 1103, 1208, 1602
Hpy8I GTNNAC 9 cut(s) 201, 613, 907, 1145, 1159, 1321, 1520, 1539, 1702
Hpy99I CGWCG 1 cut(s) 1723
HpyAV CCTTC 7 cut(s) 514, 570, 859, 1187, 1503, 1561, 1604
HpyCH4III ACNGT 2 cut(s) 49, 1304
HpyCH4IV ACGT 2 cut(s) 163, 1535
HpyF10VI GCNNNNNNNGC 1 cut(s) 1035
HpyF3I CTNAG 7 cut(s) 106, 128, 204, 760, 1181, 1505, 1542
HpySE526I ACGT 2 cut(s) 163, 1535
HspAI GCGC 1 cut(s) 1029
KpnI GGTACC 1 cut(s) 1080
Ksp22I TGATCA 4 cut(s) 220, 235, 468, 1105
KspAI GTTAAC 1 cut(s) 613
LguI GCTCTTC 1 cut(s) 1767
LmnI GCTCC 2 cut(s) 115, 1105
Lsp1109I GCAGC 4 cut(s) 303, 403, 940, 1438
LweI GCATC 2 cut(s) 403, 1363
MaeI CTAG 4 cut(s) 99, 1263, 1445, 1652
MaeII ACGT 2 cut(s) 163, 1535
MaeIII GTNAC 6 cut(s) 28, 86, 679, 873, 971, 1466
MboII GAAGA 7 cut(s) 27, 257, 631, 764, 1260, 1374, 1591
MflI RGATCY 3 cut(s) 388, 756, 1110
MhlI GDGCHC 2 cut(s) 203, 1560
MluCI AATT 4 cut(s) 41, 568, 693, 1368
MmeI TCCRAC 3 cut(s) 252, 390, 1544
Mph1103I ATGCAT 2 cut(s) 1378, 1767
MroXI GAANNNNTTC 2 cut(s) 1172, 1593
MseI TTAA 7 cut(s) 11, 488, 590, 612, 828, 1308, 1367
MslI CAYNNNNRTG 3 cut(s) 812, 1152, 1347
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 4 cut(s) 251, 462, 773, 1683
MvaI CCWGG 3 cut(s) 462, 773, 1683
MwoI GCNNNNNNNGC 1 cut(s) 1035
NciI CCSGG 1 cut(s) 251
NcoI CCATGG 1 cut(s) 1072
NlaIV GGNNCC 2 cut(s) 558, 1078
NmuCI GTSAC 5 cut(s) 86, 679, 873, 971, 1466
NsiI ATGCAT 2 cut(s) 1378, 1767
NspI RCATGY 4 cut(s) 704, 1065, 1189, 1765
PaeI GCATGC 2 cut(s) 704, 1189
PciSI GCTCTTC 1 cut(s) 1767
PdmI GAANNNNTTC 2 cut(s) 1172, 1593
PfeI GAWTC 4 cut(s) 896, 994, 1285, 1339
Pfl23II CGTACG 1 cut(s) 1299
PflFI GACNNNGTC 1 cut(s) 92
PkrI GCNGC 4 cut(s) 293, 418, 955, 1453
Ppu21I YACGTR 1 cut(s) 1536
PpuMI RGGWCCY 1 cut(s) 557
PshBI ATTAAT 1 cut(s) 1367
PsiI TTATAA 1 cut(s) 582
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 3 cut(s) 460, 771, 1681
PspGI CCWGG 3 cut(s) 460, 771, 1681
PspLI CGTACG 1 cut(s) 1299
PspN4I GGNNCC 2 cut(s) 558, 1078
PspPI GGNCC 1 cut(s) 557
PspPPI RGGWCCY 1 cut(s) 557
PsuI RGATCY 3 cut(s) 388, 756, 1110
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 6 cut(s) 635, 805, 908, 1078, 1301, 1459
RsaNI GTAC 6 cut(s) 634, 804, 907, 1077, 1300, 1458
RseI CAYNNNNRTG 3 cut(s) 812, 1152, 1347
SapI GCTCTTC 1 cut(s) 1767
SaqAI TTAA 7 cut(s) 11, 488, 590, 612, 828, 1308, 1367
SatI GCNGC 4 cut(s) 292, 417, 954, 1452
Sau96I GGNCC 1 cut(s) 557
ScrFI CCNGG 4 cut(s) 251, 462, 773, 1683
SduI GDGCHC 2 cut(s) 203, 1560
SfaNI GCATC 2 cut(s) 403, 1363
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 3 cut(s) 812, 1152, 1347
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SnaBI TACGTA 1 cut(s) 1536
SphI GCATGC 2 cut(s) 704, 1189
Sse9I AATT 4 cut(s) 41, 568, 693, 1368
SsiI CCGC 2 cut(s) 524, 1474
SspMI CTAG 4 cut(s) 99, 1263, 1445, 1652
StyD4I CCNGG 4 cut(s) 249, 460, 771, 1681
StyI CCWWGG 2 cut(s) 786, 1072
TaaI ACNGT 2 cut(s) 49, 1304
TaiI ACGT 2 cut(s) 166, 1538
TaqI TCGA 5 cut(s) 159, 516, 935, 1573, 1601
TasI AATT 4 cut(s) 41, 568, 693, 1368
TatI WGTACW 2 cut(s) 906, 1457
TfiI GAWTC 4 cut(s) 896, 994, 1285, 1339
Tru1I TTAA 7 cut(s) 11, 488, 590, 612, 828, 1308, 1367
Tru9I TTAA 7 cut(s) 11, 488, 590, 612, 828, 1308, 1367
TscAI CASTG 4 cut(s) 52, 208, 613, 1273
TseFI GTSAC 5 cut(s) 86, 679, 873, 971, 1466
TseI GCWGC 4 cut(s) 291, 416, 953, 1451
Tsp45I GTSAC 5 cut(s) 86, 679, 873, 971, 1466
TspDTI ATGAA 6 cut(s) 300, 665, 955, 1331, 1407, 1512
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 4 cut(s) 52, 208, 613, 1273
Tth111I GACNNNGTC 1 cut(s) 92
VneI GTGCAC 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 557
VspI ATTAAT 1 cut(s) 1367
XceI RCATGY 4 cut(s) 704, 1065, 1189, 1765
XmiI GTMKAC 2 cut(s) 1144, 1538
XmnI GAANNNNTTC 2 cut(s) 1172, 1593
XspI CTAG 4 cut(s) 99, 1263, 1445, 1652
Zsp2I ATGCAT 2 cut(s) 1378, 1767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.