pycom08g11270

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009978
Physical Location & Seq
Reverse (-)
9560961 .. 9564118
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11270.1

Sequence Viewer

Length: 1806 bp
ATGGCATATATCAAGCCGGTTGTACCTCTCAAGTTTGTAAATGCCAGTAAGCACCAAAACCAGGACAAGATCTCGGCTGCTAGAGGCGCAAATTTCGGAGGAAGACAGCCGAAGGAACTTGTAATCGCGTGCGGAGAATCGTCGCCGTCCCACATTGCCCTTCTGAGATGTTTGAATCGAACCCTCGACTCCATAAAGCACAACATTGCTCGCATTTTTGTCAACATTTTGTGCTGCCAAGCTGACTCTGATGCCAACGCCAGGGGTAAGGATCTACTTCCAACTACTTTTGAGGATTTCATGAAAAATTGGAATGAGAATTACCATTTGAGTGCTCAACATTGGTTTTCAAAGAAAAATAATTCCGACGGTGAACAAGATCCACATGCAGTTTTCAATGCGTTAGATGCAATGTTGAAGGACAGTTTGGAACGTCTGAAAACAATGAGGGAAAGCATATCAATGACAGAGATAGGCTTTATAAGTTGCACTTCGAAAGTTAACTACATGAATCATGTGGGCATCATAAGGGTTTTATGCATGGATGGTAAGTTGGGAGCAGCTTTTTGGCTTCGGAGAAAAATGATAAAGAAAGGCTTTGTTCCTGATGTTTTGACGCACAATTATCTTCTAAACAGACTATGTAAAACTGGTGACTTGGAGAAGGCAGATTGGCTTATTAGAGAGATGTTAGAAATTGGCCCTTCTCCCAACTGCGCCACATACAACACGTTTATTAAGGGTTATTGCCTTATTGATGATGTGGACAAAGCTCTTTATCTTTTCTCCACGATGAGTAACAGTGGTATTAGTCCGAATAGAGTCACTTGCAACATACTTGTACATGCATTGTGCAAGAAAGGTCTTATGGAGAATGCAAAGAAGCTTCTTAACAAGGTATTGGGTGTTGATAATGACAAGACAGCATTAGATCTAATCACCTCAACTACACTGATGGATGGTTATCTTAAAAACGGGAATACAGTTCAGGCACTTCGTATTTGGGATGACATGGTCCAAATGAATACCCAAATAGATGTCGTTGCTTATAATGTTCTCATCCATGGATTTTGTTTGAGTCAAGACATGAACCTTGCTTACGGTTCCTTTTGTGAAATGATCGAAAGAGGATTACTTCCTAATGTTATTACTTTTAATACACTTATAAGTGGTCTTTGCAAAGAAGGAAAATTAGAGGAGGCTTGCTACGTCCATGAAACTATGTCAAAAATGGGAGTTATTCCAGATCAAATTTCGTACAAAATAATCATTCAGGGCCTGTGTATTAAAGGAGATGTTTTCAGAGCTAACAAGTTTCTTCTTTGTATGCTAGAGAAGTCAATGGTGCCTGAGCCTCTACCATGGAATTGTATTATCGATTGTTATGGAAGATATGGGGATCTTAGTACTGCACTCGCAGTCAAGGATCGTATGTTAGCTTCTGGTGTTCAACCAAATGTGTATACTTACAATGCATCGATCCACGCACAAGTAAAAGGAGGAAACATTGAACGTGGTCTTGTTCTCAAAAAGGAAATGCTTATGTCTGGTCTTTGTCCTGATGTGGTCACTTATAATTTGTTGATAGGTGCTGCTTGTAAGTTAGGGTACATTTATTTTGCATTCCGGTTACATGATGAAATGCTAAGAATAGGATTTGATCCAGATATATTTACCTATGCTGCACTAATCAGAGGGCACTGCATGAGAGGAAACATGAAGGAGGCAGAAGAGCTTTTTACAAAGATGCGGGAATCTGGTTTACCCATCGATCATGTTCCATATCAGATGCTTTTCAAGAAGTAA

Protein Analysis

602

Amino Acids

67.61

Weight (kDa)

8.41

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 175 - 216 1.4e-07 PPR repeat family
PPR_2 PF13041 207 - 250 1.9e-09 PPR repeat family
PPR_3 PF13812 226 - 284 3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 234 - 265 1.1e-08 PPR repeat
PPR_2 PF13041 237 - 286 1.4e-14 PPR repeat family
TPR_24 PF23276 242 - 368 4.4e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 269 - 299 8.8e-08 PPR repeat
PPR_2 PF13041 311 - 358 3.9e-12 PPR repeat family
PPR_3 PF13812 333 - 390 2.7e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 346 - 394 7.1e-16 PPR repeat family
PPR_3 PF13812 372 - 425 1.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 376 - 408 2e-13 PPR repeat
PPR_2 PF13041 380 - 428 1e-16 PPR repeat family
PPR PF01535 383 - 413 2.6e-07 PPR repeat
PPR_2 PF13041 455 - 496 3.9e-09 PPR repeat family
PPR_long PF17177 463 - 600 4.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 474 - 531 1.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 487 - 534 1.1e-08 PPR repeat family
PPR_1 PF12854 517 - 548 7.8e-08 PPR repeat
PPR_2 PF13041 520 - 568 2.5e-13 PPR repeat family
PPR_3 PF13812 543 - 588 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 552 - 584 5.6e-10 PPR repeat
PPR_2 PF13041 555 - 600 3.7e-10 PPR repeat family
PPR PF01535 558 - 588 3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 482, 1050, 1166, 1575
AccB1I GGYRCC 1 cut(s) 1345
AccI GTMKAC 1 cut(s) 1463
AccII CGCG 1 cut(s) 128
AciI CCGC 2 cut(s) 132, 1750
AclWI GGATC 6 cut(s) 279, 374, 1407, 1434, 1474, 1655
AcsI RAATTY 2 cut(s) 91, 1251
AfaI GTAC 5 cut(s) 24, 843, 1259, 1408, 1610
AfiI CCNNNNNNNGG 2 cut(s) 61, 261
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 7 cut(s) 175, 351, 397, 418, 1451, 1511, 1798
AjnI CCWGG 2 cut(s) 60, 260
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 242, 563, 773, 886, 1307, 1439, 1735
AluI AGCT 7 cut(s) 242, 563, 773, 886, 1307, 1439, 1735
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 6 cut(s) 279, 374, 1407, 1434, 1474, 1655
AlwNI CAGNNNCTG 1 cut(s) 1279
AoxI GGCC 2 cut(s) 700, 1276
ApeKI GCWGC 5 cut(s) 77, 234, 560, 1592, 1682
ApoI RAATTY 2 cut(s) 91, 1251
AspLEI GCGC 2 cut(s) 89, 719
AspS9I GGNCC 3 cut(s) 701, 1015, 1276
AsuHPI GGTGA 3 cut(s) 383, 665, 931
AsuII TTCGAA 1 cut(s) 494
AvaII GGWCC 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 1701
BanI GGYRCC 1 cut(s) 1345
BarI GAAGNNNNNNTAC 2 cut(s) 1423, 1455
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 5 cut(s) 64, 221, 572, 1579, 1669
BccI CCATC 4 cut(s) 539, 949, 953, 1775
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 2 cut(s) 62, 262
BfaI CTAG 2 cut(s) 81, 1331
BglII AGATCT 2 cut(s) 69, 931
BisI GCNGC 5 cut(s) 78, 235, 561, 1593, 1683
BlsI GCNGC 5 cut(s) 79, 236, 562, 1594, 1684
BmcAI AGTACT 1 cut(s) 1408
Bme1390I CCNGG 2 cut(s) 62, 262
Bme18I GGWCC 1 cut(s) 1015
BmgT120I GGNCC 3 cut(s) 701, 1015, 1276
BmiI GGNNCC 2 cut(s) 1105, 1347
BmrFI CCNGG 2 cut(s) 62, 262
BmsI GCATC 6 cut(s) 241, 397, 531, 1484, 1737, 1779
BpiI GAAGAC 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 1350
Bpu14I TTCGAA 1 cut(s) 494
BpuEI CTTGAG 1 cut(s) 14
Bsa29I ATCGAT 3 cut(s) 1377, 1478, 1770
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 3 cut(s) 261, 1063, 1361
BsaWI WCCGGW 1 cut(s) 1626
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 261
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 45, 655
Bse3DI GCAATG 3 cut(s) 153, 204, 417
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 2 cut(s) 62, 262
BseCI ATCGAT 3 cut(s) 1377, 1478, 1770
BseDI CCNNGG 3 cut(s) 261, 1063, 1361
BseGI GGATG 4 cut(s) 550, 964, 1012, 1059
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 2 cut(s) 61, 261
BseMI GCAATG 3 cut(s) 153, 204, 417
BseMII CTCAG 2 cut(s) 155, 1341
BseNI ACTGG 2 cut(s) 45, 655
BseRI GAGGAG 1 cut(s) 1211
BseSI GKGCMC 1 cut(s) 1701
BseXI GCAGC 5 cut(s) 64, 221, 572, 1579, 1669
BsgI GTGCAG 2 cut(s) 1395, 1668
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 2 cut(s) 702, 1278
BshNI GGYRCC 1 cut(s) 1345
BshVI ATCGAT 3 cut(s) 1377, 1478, 1770
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 2 cut(s) 17, 1627
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 61, 261
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 2 cut(s) 880, 1622
BsnI GGCC 2 cut(s) 702, 1278
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 337, 1701
Bsp1407I TGTACA 1 cut(s) 841
Bsp19I CCATGG 2 cut(s) 1063, 1361
BspACI CCGC 2 cut(s) 132, 1750
BspANI GGCC 2 cut(s) 702, 1278
BspCNI CTCAG 2 cut(s) 156, 1342
BspDI ATCGAT 3 cut(s) 1377, 1478, 1770
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 1105, 1347
BspPI GGATC 6 cut(s) 279, 374, 1407, 1434, 1474, 1655
BspQI GCTCTTC 1 cut(s) 1725
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 1345
BsrDI GCAATG 3 cut(s) 153, 204, 417
BsrFI RCCGGY 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 841
BsrI ACTGG 2 cut(s) 45, 655
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 261, 1063, 1361
BssNAI GTATAC 1 cut(s) 1464
BssT1I CCWWGG 2 cut(s) 1063, 1361
Bst1107I GTATAC 1 cut(s) 1464
Bst2UI CCWGG 2 cut(s) 62, 262
Bst4CI ACNGT 5 cut(s) 371, 425, 803, 985, 1103
Bst6I CTCTTC 1 cut(s) 1725
BstAUI TGTACA 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 3 cut(s) 130, 211, 1204
BstDEI CTNAG 4 cut(s) 164, 1350, 1403, 1646
BstDSI CCRYGG 2 cut(s) 1063, 1361
BstF5I GGATG 4 cut(s) 550, 964, 1012, 1059
BstFNI CGCG 1 cut(s) 128
BstHHI GCGC 2 cut(s) 89, 719
BstMWI GCNNNNNNNGC 2 cut(s) 86, 407
BstNI CCWGG 2 cut(s) 62, 262
BstNSI RCATGY 2 cut(s) 389, 848
BstSCI CCNGG 2 cut(s) 60, 260
BstSLI GKGCMC 1 cut(s) 1701
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 5 cut(s) 64, 221, 572, 1579, 1669
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 5 cut(s) 69, 271, 379, 931, 1399
BstYI RGATCY 5 cut(s) 69, 271, 379, 931, 1399
BstZ17I GTATAC 1 cut(s) 1464
Bsu15I ATCGAT 3 cut(s) 1377, 1478, 1770
BsuRI GGCC 2 cut(s) 702, 1278
BsuTUI ATCGAT 3 cut(s) 1377, 1478, 1770
BtgI CCRYGG 2 cut(s) 1063, 1361
BtsCI GGATG 4 cut(s) 550, 964, 1012, 1059
BtsI GCAGTG 1 cut(s) 1699
BtsIMutI CAGTG 3 cut(s) 808, 950, 1699
Cac8I GCNNGC 3 cut(s) 130, 211, 1204
CaiI CAGNNNCTG 1 cut(s) 1279
CciI TCATGA 1 cut(s) 300
CfoI GCGC 2 cut(s) 89, 719
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 701, 1015, 1276
ClaI ATCGAT 3 cut(s) 1377, 1478, 1770
CseI GACGC 1 cut(s) 625
Csp6I GTAC 5 cut(s) 23, 842, 1258, 1407, 1609
CviQI GTAC 5 cut(s) 23, 842, 1258, 1407, 1609
DdeI CTNAG 4 cut(s) 164, 1350, 1403, 1646
Eam1104I CTCTTC 1 cut(s) 1725
EarI CTCTTC 1 cut(s) 1725
Eco130I CCWWGG 2 cut(s) 1063, 1361
Eco47I GGWCC 1 cut(s) 1015
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRII CCWGG 2 cut(s) 60, 260
EcoT14I CCWWGG 2 cut(s) 1063, 1361
EcoT22I ATGCAT 3 cut(s) 542, 850, 1477
ErhI CCWWGG 2 cut(s) 1063, 1361
FalI AAGNNNNNCTT 4 cut(s) 475, 507, 581, 613
FaqI GGGAC 1 cut(s) 133
FauI CCCGC 1 cut(s) 1743
FblI GTMKAC 1 cut(s) 1463
Fnu4HI GCNGC 5 cut(s) 78, 235, 561, 1593, 1683
FokI GGATG 4 cut(s) 557, 971, 1019, 1046
Fsp4HI GCNGC 5 cut(s) 78, 235, 561, 1593, 1683
FspBI CTAG 2 cut(s) 81, 1331
GlaI GCGC 2 cut(s) 88, 718
GluI GCNGC 5 cut(s) 78, 235, 561, 1593, 1683
HaeIII GGCC 2 cut(s) 702, 1278
HapII CCGG 2 cut(s) 17, 1627
HgaI GACGC 1 cut(s) 625
HhaI GCGC 2 cut(s) 89, 719
Hin6I GCGC 2 cut(s) 87, 717
HinP1I GCGC 2 cut(s) 87, 717
HincII GTYRAC 2 cut(s) 223, 502
HindII GTYRAC 2 cut(s) 223, 502
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 8 cut(s) 137, 175, 188, 245, 511, 822, 1078, 1754
HpaI GTTAAC 1 cut(s) 502
HpaII CCGG 2 cut(s) 17, 1627
HphI GGTGA 3 cut(s) 383, 665, 931
Hpy166II GTNNAC 6 cut(s) 223, 374, 502, 766, 1464, 1763
Hpy188III TCNNGA 7 cut(s) 301, 605, 1082, 1244, 1559, 1664, 1798
Hpy8I GTNNAC 6 cut(s) 223, 374, 502, 766, 1464, 1763
Hpy99I CGWCG 2 cut(s) 145, 371
HpyAV CCTTC 7 cut(s) 106, 170, 412, 658, 714, 1178, 1714
HpyCH4III ACNGT 5 cut(s) 371, 425, 803, 985, 1103
HpyCH4IV ACGT 4 cut(s) 433, 731, 1209, 1513
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 407
HpyF3I CTNAG 4 cut(s) 164, 1350, 1403, 1646
HpySE526I ACGT 4 cut(s) 433, 731, 1209, 1513
HspAI GCGC 2 cut(s) 87, 717
KspAI GTTAAC 1 cut(s) 502
LguI GCTCTTC 1 cut(s) 1725
LmnI GCTCC 1 cut(s) 557
Lsp1109I GCAGC 5 cut(s) 64, 221, 572, 1579, 1669
LweI GCATC 6 cut(s) 241, 397, 531, 1484, 1737, 1779
MaeI CTAG 2 cut(s) 81, 1331
MaeII ACGT 4 cut(s) 433, 731, 1209, 1513
MaeIII GTNAC 5 cut(s) 653, 797, 823, 1567, 1629
MboII GAAGA 5 cut(s) 114, 620, 1310, 1401, 1742
MflI RGATCY 5 cut(s) 69, 271, 379, 931, 1399
MhlI GDGCHC 2 cut(s) 337, 1701
MlyI GAGTC 4 cut(s) 182, 239, 831, 1087
MmeI TCCRAC 2 cut(s) 305, 390
Mph1103I ATGCAT 3 cut(s) 542, 850, 1477
MseI TTAA 6 cut(s) 501, 738, 891, 969, 1155, 1287
MslI CAYNNNNRTG 2 cut(s) 330, 461
MspI CCGG 2 cut(s) 17, 1627
MspR9I CCNGG 2 cut(s) 62, 262
Mva1269I GAATGC 2 cut(s) 880, 1622
MvaI CCWGG 2 cut(s) 62, 262
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 86, 407
NcoI CCATGG 2 cut(s) 1063, 1361
NlaIV GGNNCC 2 cut(s) 1105, 1347
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 3 cut(s) 653, 823, 1567
NsiI ATGCAT 3 cut(s) 542, 850, 1477
NspI RCATGY 2 cut(s) 389, 848
NspV TTCGAA 1 cut(s) 494
PagI TCATGA 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 1725
PctI GAATGC 2 cut(s) 880, 1622
PfeI GAWTC 4 cut(s) 137, 175, 511, 1754
PflFI GACNNNGTC 1 cut(s) 1013
PkrI GCNGC 5 cut(s) 79, 236, 562, 1594, 1684
PleI GAGTC 4 cut(s) 182, 239, 830, 1086
PpsI GAGTC 4 cut(s) 182, 239, 830, 1086
PsiI TTATAA 4 cut(s) 482, 1050, 1166, 1575
Psp6I CCWGG 2 cut(s) 60, 260
PspGI CCWGG 2 cut(s) 60, 260
PspN4I GGNNCC 2 cut(s) 1105, 1347
PspPI GGNCC 3 cut(s) 701, 1015, 1276
PsrI GAACNNNNNNTAC 2 cut(s) 1082, 1114
PstNI CAGNNNCTG 1 cut(s) 1279
PsuI RGATCY 5 cut(s) 69, 271, 379, 931, 1399
PsyI GACNNNGTC 1 cut(s) 1013
RsaI GTAC 5 cut(s) 24, 843, 1259, 1408, 1610
RsaNI GTAC 5 cut(s) 23, 842, 1258, 1407, 1609
RseI CAYNNNNRTG 2 cut(s) 330, 461
SapI GCTCTTC 1 cut(s) 1725
SaqAI TTAA 6 cut(s) 501, 738, 891, 969, 1155, 1287
SatI GCNGC 5 cut(s) 78, 235, 561, 1593, 1683
Sau96I GGNCC 3 cut(s) 701, 1015, 1276
ScaI AGTACT 1 cut(s) 1408
SchI GAGTC 4 cut(s) 182, 239, 831, 1087
ScrFI CCNGG 2 cut(s) 62, 262
SduI GDGCHC 2 cut(s) 337, 1701
SfaNI GCATC 6 cut(s) 241, 397, 531, 1484, 1737, 1779
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 1 cut(s) 1015
SmiMI CAYNNNNRTG 2 cut(s) 330, 461
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 2 cut(s) 132, 1750
SspMI CTAG 2 cut(s) 81, 1331
StyD4I CCNGG 2 cut(s) 60, 260
StyI CCWWGG 2 cut(s) 1063, 1361
TaaI ACNGT 5 cut(s) 371, 425, 803, 985, 1103
TaiI ACGT 4 cut(s) 436, 734, 1212, 1516
TaqI TCGA 7 cut(s) 178, 186, 494, 1122, 1377, 1478, 1770
TatI WGTACW 2 cut(s) 841, 1406
TfiI GAWTC 4 cut(s) 137, 175, 511, 1754
Tru1I TTAA 6 cut(s) 501, 738, 891, 969, 1155, 1287
Tru9I TTAA 6 cut(s) 501, 738, 891, 969, 1155, 1287
TscAI CASTG 3 cut(s) 808, 957, 1706
TseFI GTSAC 3 cut(s) 653, 823, 1567
TseI GCWGC 5 cut(s) 77, 234, 560, 1592, 1682
Tsp45I GTSAC 3 cut(s) 653, 823, 1567
TspDTI ATGAA 8 cut(s) 289, 317, 524, 1037, 1103, 1230, 1653, 1733
TspRI CASTG 3 cut(s) 808, 957, 1706
Tth111I GACNNNGTC 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 1015
XapI RAATTY 2 cut(s) 91, 1251
XceI RCATGY 2 cut(s) 389, 848
XmiI GTMKAC 1 cut(s) 1463
XspI CTAG 2 cut(s) 81, 1331
ZrmI AGTACT 1 cut(s) 1408
Zsp2I ATGCAT 3 cut(s) 542, 850, 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.