Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009978

Physical Location & Seq

Genomic Coordinates Reverse (-)

12705783 .. 12709556

Gene Structure 3774 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1133900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGCACTTGTAATCGCGTGCGGAGAATCGTCGCCGTCCCACATTGCCCTTCTGAGATGTTTGAATCGAACCCTCGACTCCATCAAGCACAACATTGCTCGCATTTTTGTCAACATTTTGTGCTGCCAAGCTGACTCTGATGCCAACGCCAGGGGTAAGGATCTACTTCCAACTACTTTTGAGGATTTCATGATAAATTGGAATGAGAATTACCATTTGAATGCTCAACATTGGTTTTCAAAGAAAAATAATTCCGATGGTGAACAAGATCCACATGCGGTTTTCAATGCGTTGGATGCAATGTTGAAGGACAGTTTGGAACGTCTGAAAACAATGAGGGAAAGCATATCAATGACAGAGATAGGCTTTATAAGTTGCACTTCGAAAGTTAACTACATGGATCACATGGGCATCATAAGGGTTTTATGCATGGATGGTAAGTTGGGAACAGCTTTTTGGCTTCGGAGAAAAATGATAAAGAAAGGCTTTGTTCCTGATGTTTTGACGCACAATTATCTTCTAAACGGACTATGTAAAACTGGTGACTTGGAGAAGGCAGATTGGCTTCTTAGAGAGATGTTAGAAATTGGCCCTTCTCCCAACTGCGCCACATACAACACGTTTATTAAGGGTTATTGCCTTCTTGATGATGTGGACAAAGCTCTCTATCTTTTCTCCACGATGAGTAACAGTGGTATTAGTCCGAATAGAGTCACTTGCAACATACTTGTACATGCATTGTGCAAGAAAGGTCTTATGGAGAATGCAAAGAAGCTTCTTAACAAGATATTGGATGTTGATAATGACAAGACAGCATCAGATCTAATCATTTCAACTATACTTATGGATGGTTATCTTAAAAACGGGAATACAGTTCAGGCACTTCATATTTGGGATGAAATGGTCCAAATGAATACCCAAATAGATGTCGTTGCTTATAATGTTCTCATCCATGGATTTTGTTTGAGTCGAGACATGAACCTTGCTTACGGTTCCCTTTGTGAAATGATCAAAAGAGGATTACTTCCTAATGTTATTACTTTTAATACACTTATAAGTGGTCTTTGCAAAGAAGGAAAATTAGAGGAGGCTTGCTACGTCCATGAAAGTATGTCAAAAATGGGAGTTACTCCAGATCAAATTTCATACAAAATAATCATTCAGGGCCTGTGTATTAAAGGAGATGTTTTCAGAGCTAACAAGTTTCTTCTTTGTATGCTAGAGAAGTCAATGGTGCCTGAGCCTCTACCATGGAATTGTATTATCGATTGTTATGGAAGATATGGGAATCTTAGTACTGCACTCTCAGTCAAGGATCATATGTTCGCTTCTGGTGTTCAACCAAATGTGTATACTTACAATGCGTTGATCCACGCACAAGTAAAAGGAGGAAACATTGATCGTGCTCTTGTTCTCAAAAAGGAAATGCTTATGTCTGGTCTTTGTCCTGATGTGGTCACTTATAATTTGTTGATAGGTGCTGCTTGTAAGTTAGGGCACATTTATTTTGCATTCCGGTTGTATGATGAAATGCTGAGAATAGGATTTGATCCAGATATATTTACCTATGCTGCACTAATCAGAGGGCACTGCATGAGAGGAAACATGAAGGAGGCAGAAGAGCTTTTTACAAAGATGCGGGAATCTGGTTTACCCATTGATCATGTTCCATATCAGATACTTTTCAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

565

Amino Acids

63.58

Weight (kDa)

7.18

Isoelectric Point (pI)

37.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	138 - 179	1.7e-08	PPR repeat family
PPR_3	PF13812	156 - 212	1.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	161 - 193	1.9e-07	PPR repeat
PPR	PF01535	169 - 197	6.4e-06	PPR repeat
PPR_2	PF13041	170 - 213	9e-11	PPR repeat family
PPR_3	PF13812	189 - 247	1.5e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	197 - 228	9.7e-09	PPR repeat
PPR_2	PF13041	200 - 249	1.1e-14	PPR repeat family
TPR_24	PF23276	205 - 301	6.1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	232 - 262	6e-08	PPR repeat
PPR_2	PF13041	277 - 321	3.9e-11	PPR repeat family
PPR_3	PF13812	297 - 353	3.2e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	309 - 357	7.9e-16	PPR repeat family
PPR_3	PF13812	335 - 388	1.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	339 - 371	1.6e-13	PPR repeat
PPR_2	PF13041	343 - 391	2.5e-17	PPR repeat family
PPR	PF01535	346 - 376	8.6e-08	PPR repeat
PPR_1	PF12854	374 - 403	1e-05	PPR repeat
PPR_2	PF13041	418 - 459	2.1e-10	PPR repeat family
PPR_long	PF17177	427 - 562	7.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	436 - 494	2.2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	445 - 476	1.6e-06	PPR repeat
PPR_2	PF13041	452 - 497	8.3e-11	PPR repeat family
PPR_1	PF12854	480 - 511	2.2e-08	PPR repeat
PPR_2	PF13041	483 - 531	6.2e-15	PPR repeat family
PPR	PF01535	486 - 516	7.9e-06	PPR repeat
PPR_3	PF13812	506 - 551	1.7e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	515 - 547	5.2e-10	PPR repeat
PPR_2	PF13041	518 - 563	7.1e-11	PPR repeat family
PPR	PF01535	521 - 551	2.8e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24830
fragaria_vesca	FvH4_2g30660 FvH4_2g30660 FvH4_2g30660 FvH4_2g30660 FvH4_2g30660
malus_domestica	MD08G1133900.v1.1 MD15G1110300.v1.1
prunus_persica	Prupe.1G462500_v2.0.a1
pyrus_communis	pycom08g11270
rosa_chinensis	RchiOBHm_Chr6g0312241
rosa_laevigata	RLG00000010339
rosa_multiflora	Rmu_sc0010924.1_g000002 Rmu_ssc0000424.1_g000002
rosa_roxburghii	Rroxscaffold_7G00156920
rosa_rugosa	Rorug06G0394300
rosa_samantha	Rh6AG511300 Rh6BG522300 Rh6CG528100 Rh6DG513600
rosa_wichuraiana	Rw6G044470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	371, 939, 1055, 1464
AccB1I	GGYRCC	1 cut(s)	1234
AccI	GTMKAC	1 cut(s)	1352
AccII	CGCG	1 cut(s)	17
AciI	CCGC	3 cut(s)	21, 278, 1639
AclWI	GGATC	6 cut(s)	168, 263, 408, 1323, 1363, 1544
AcsI	RAATTY	1 cut(s)	1140
AfaI	GTAC	2 cut(s)	732, 1297
AfiI	CCNNNNNNNGG	1 cut(s)	150
AflIII	ACRYGT	1 cut(s)	618
AgsI	TTSAA	8 cut(s)	64, 220, 240, 286, 307, 834, 1340, 1687
AjnI	CCWGG	1 cut(s)	149
AjuI	GAANNNNNNNTTGG	4 cut(s)	299, 331, 439, 471
AloI	GAACNNNNNNTCC	2 cut(s)	1307, 1339
AluBI	AGCT	6 cut(s)	131, 452, 662, 775, 1196, 1624
AluI	AGCT	6 cut(s)	131, 452, 662, 775, 1196, 1624
Alw21I	GWGCWC	1 cut(s)	1408
Alw26I	GTCTC	1 cut(s)	966
AlwI	GGATC	6 cut(s)	168, 263, 408, 1323, 1363, 1544
AlwNI	CAGNNNCTG	1 cut(s)	1168
AoxI	GGCC	2 cut(s)	589, 1165
ApeKI	GCWGC	3 cut(s)	123, 1481, 1571
ApoI	RAATTY	1 cut(s)	1140
AspLEI	GCGC	1 cut(s)	608
AspS9I	GGNCC	3 cut(s)	590, 904, 1165
AsuHPI	GGTGA	2 cut(s)	272, 554
AsuII	TTCGAA	1 cut(s)	383
AvaII	GGWCC	1 cut(s)	904
BaeGI	GKGCMC	2 cut(s)	1500, 1590
BanI	GGYRCC	1 cut(s)	1234
Bbv12I	GWGCWC	1 cut(s)	1408
BbvI	GCAGC	3 cut(s)	110, 1468, 1558
BccI	CCATC	4 cut(s)	89, 251, 428, 842
BceAI	ACGGC	1 cut(s)	19
BciT130I	CCWGG	1 cut(s)	151
BclI	TGATCA	2 cut(s)	1008, 1660
BcoDI	GTCTC	1 cut(s)	966
BfaI	CTAG	1 cut(s)	1220
BglII	AGATCT	1 cut(s)	820
BisI	GCNGC	3 cut(s)	124, 1482, 1572
BlsI	GCNGC	3 cut(s)	125, 1483, 1573
BmcAI	AGTACT	1 cut(s)	1297
Bme1390I	CCNGG	1 cut(s)	151
Bme18I	GGWCC	1 cut(s)	904
BmgT120I	GGNCC	3 cut(s)	590, 904, 1165
BmiI	GGNNCC	2 cut(s)	994, 1236
BmrFI	CCNGG	1 cut(s)	151
BmsI	GCATC	5 cut(s)	130, 286, 420, 824, 1626
BpmI	CTGGAG	1 cut(s)	1116
Bpu10I	CCTNAGC	1 cut(s)	1239
Bpu14I	TTCGAA	1 cut(s)	383
Bsa29I	ATCGAT	1 cut(s)	1266
BsaBI	GATNNNNATC	1 cut(s)	852
BsaJI	CCNNGG	3 cut(s)	150, 952, 1250
BsaWI	WCCGGW	1 cut(s)	1515
Bsc4I	CCNNNNNNNGG	1 cut(s)	150
Bse1I	ACTGG	1 cut(s)	544
Bse3DI	GCAATG	3 cut(s)	42, 93, 306
Bse8I	GATNNNNATC	1 cut(s)	852
BseBI	CCWGG	1 cut(s)	151
BseCI	ATCGAT	1 cut(s)	1266
BseDI	CCNNGG	3 cut(s)	150, 952, 1250
BseGI	GGATG	6 cut(s)	301, 439, 799, 853, 901, 948
BseJI	GATNNNNATC	1 cut(s)	852
BseLI	CCNNNNNNNGG	1 cut(s)	150
BseMI	GCAATG	3 cut(s)	42, 93, 306
BseMII	CTCAG	4 cut(s)	44, 1230, 1320, 1526
BseNI	ACTGG	1 cut(s)	544
BseRI	GAGGAG	1 cut(s)	1100
BseSI	GKGCMC	2 cut(s)	1500, 1590
BseXI	GCAGC	3 cut(s)	110, 1468, 1558
BsgI	GTGCAG	2 cut(s)	1284, 1557
Bsh1236I	CGCG	1 cut(s)	17
BshFI	GGCC	2 cut(s)	591, 1167
BshNI	GGYRCC	1 cut(s)	1234
BshVI	ATCGAT	1 cut(s)	1266
BsiHKAI	GWGCWC	1 cut(s)	1408
BsiSI	CCGG	1 cut(s)	1516
BslFI	GGGAC	1 cut(s)	22
BslI	CCNNNNNNNGG	1 cut(s)	150
BsmAI	GTCTC	1 cut(s)	966
BsmFI	GGGAC	1 cut(s)	22
BsmI	GAATGC	3 cut(s)	226, 769, 1511
BsnI	GGCC	2 cut(s)	591, 1167
Bsp119I	TTCGAA	1 cut(s)	383
Bsp1286I	GDGCHC	3 cut(s)	1408, 1500, 1590
Bsp1407I	TGTACA	1 cut(s)	730
Bsp19I	CCATGG	2 cut(s)	952, 1250
BspACI	CCGC	3 cut(s)	21, 278, 1639
BspANI	GGCC	2 cut(s)	591, 1167
BspCNI	CTCAG	4 cut(s)	45, 1231, 1319, 1527
BspDI	ATCGAT	1 cut(s)	1266
BspFNI	CGCG	1 cut(s)	17
BspHI	TCATGA	1 cut(s)	189
BspLI	GGNNCC	2 cut(s)	994, 1236
BspPI	GGATC	6 cut(s)	168, 263, 408, 1323, 1363, 1544
BspQI	GCTCTTC	1 cut(s)	1614
BspT104I	TTCGAA	1 cut(s)	383
BspT107I	GGYRCC	1 cut(s)	1234
BsrDI	GCAATG	3 cut(s)	42, 93, 306
BsrGI	TGTACA	1 cut(s)	730
BsrI	ACTGG	1 cut(s)	544
BssECI	CCNNGG	3 cut(s)	150, 952, 1250
BssNAI	GTATAC	1 cut(s)	1353
BssT1I	CCWWGG	2 cut(s)	952, 1250
Bst1107I	GTATAC	1 cut(s)	1353
Bst2UI	CCWGG	1 cut(s)	151
Bst4CI	ACNGT	4 cut(s)	314, 692, 874, 992
Bst6I	CTCTTC	1 cut(s)	1614
BstAUI	TGTACA	1 cut(s)	730
BstBI	TTCGAA	1 cut(s)	383
BstC8I	GCNNGC	3 cut(s)	19, 100, 1093
BstDEI	CTNAG	6 cut(s)	53, 569, 1239, 1292, 1306, 1535
BstDSI	CCRYGG	2 cut(s)	952, 1250
BstF5I	GGATG	6 cut(s)	301, 439, 799, 853, 901, 948
BstFNI	CGCG	1 cut(s)	17
BstHHI	GCGC	1 cut(s)	608
BstMAI	GTCTC	1 cut(s)	966
BstMWI	GCNNNNNNNGC	1 cut(s)	296
BstNI	CCWGG	1 cut(s)	151
BstNSI	RCATGY	2 cut(s)	278, 737
BstSCI	CCNGG	1 cut(s)	149
BstSLI	GKGCMC	2 cut(s)	1500, 1590
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	3 cut(s)	110, 1468, 1558
BstX2I	RGATCY	3 cut(s)	160, 268, 820
BstYI	RGATCY	3 cut(s)	160, 268, 820
BstZ17I	GTATAC	1 cut(s)	1353
Bsu15I	ATCGAT	1 cut(s)	1266
BsuRI	GGCC	2 cut(s)	591, 1167
BsuTUI	ATCGAT	1 cut(s)	1266
BtgI	CCRYGG	2 cut(s)	952, 1250
BtsCI	GGATG	6 cut(s)	301, 439, 799, 853, 901, 948
BtsI	GCAGTG	1 cut(s)	1588
BtsIMutI	CAGTG	2 cut(s)	697, 1588
Cac8I	GCNNGC	3 cut(s)	19, 100, 1093
CaiI	CAGNNNCTG	1 cut(s)	1168
CciI	TCATGA	1 cut(s)	189
CfoI	GCGC	1 cut(s)	608
Cfr13I	GGNCC	3 cut(s)	590, 904, 1165
ClaI	ATCGAT	1 cut(s)	1266
CseI	GACGC	1 cut(s)	514
Csp6I	GTAC	2 cut(s)	731, 1296
CviQI	GTAC	2 cut(s)	731, 1296
DdeI	CTNAG	6 cut(s)	53, 569, 1239, 1292, 1306, 1535
Eam1104I	CTCTTC	1 cut(s)	1614
EarI	CTCTTC	1 cut(s)	1614
Eco130I	CCWWGG	2 cut(s)	952, 1250
Eco47I	GGWCC	1 cut(s)	904
EcoO109I	RGGNCCY	1 cut(s)	1165
EcoRII	CCWGG	1 cut(s)	149
EcoT14I	CCWWGG	2 cut(s)	952, 1250
EcoT22I	ATGCAT	2 cut(s)	431, 739
ErhI	CCWWGG	2 cut(s)	952, 1250
FalI	AAGNNNNNCTT	4 cut(s)	364, 396, 470, 502
FaqI	GGGAC	1 cut(s)	22
FauI	CCCGC	1 cut(s)	1632
FauNDI	CATATG	1 cut(s)	1320
FbaI	TGATCA	2 cut(s)	1008, 1660
FblI	GTMKAC	1 cut(s)	1352
Fnu4HI	GCNGC	3 cut(s)	124, 1482, 1572
FokI	GGATG	6 cut(s)	308, 446, 806, 860, 908, 935
Fsp4HI	GCNGC	3 cut(s)	124, 1482, 1572
FspBI	CTAG	1 cut(s)	1220
GlaI	GCGC	1 cut(s)	607
GluI	GCNGC	3 cut(s)	124, 1482, 1572
GsuI	CTGGAG	1 cut(s)	1116
HaeIII	GGCC	2 cut(s)	591, 1167
HapII	CCGG	1 cut(s)	1516
HgaI	GACGC	1 cut(s)	514
HhaI	GCGC	1 cut(s)	608
Hin6I	GCGC	1 cut(s)	606
HinP1I	GCGC	1 cut(s)	606
HincII	GTYRAC	2 cut(s)	112, 391
HindII	GTYRAC	2 cut(s)	112, 391
HindIII	AAGCTT	1 cut(s)	773
HinfI	GANTC	8 cut(s)	26, 64, 77, 134, 711, 967, 1288, 1643
HpaI	GTTAAC	1 cut(s)	391
HpaII	CCGG	1 cut(s)	1516
HphI	GGTGA	2 cut(s)	272, 554
Hpy166II	GTNNAC	6 cut(s)	112, 263, 391, 655, 1353, 1652
Hpy188III	TCNNGA	8 cut(s)	190, 494, 644, 971, 1133, 1448, 1553, 1687
Hpy8I	GTNNAC	6 cut(s)	112, 263, 391, 655, 1353, 1652
Hpy99I	CGWCG	1 cut(s)	34
HpyAV	CCTTC	7 cut(s)	59, 301, 547, 603, 650, 1067, 1603
HpyCH4III	ACNGT	4 cut(s)	314, 692, 874, 992
HpyCH4IV	ACGT	3 cut(s)	322, 620, 1098
HpyF10VI	GCNNNNNNNGC	1 cut(s)	296
HpyF3I	CTNAG	6 cut(s)	53, 569, 1239, 1292, 1306, 1535
HpySE526I	ACGT	3 cut(s)	322, 620, 1098
HspAI	GCGC	1 cut(s)	606
Ksp22I	TGATCA	2 cut(s)	1008, 1660
KspAI	GTTAAC	1 cut(s)	391
LguI	GCTCTTC	1 cut(s)	1614
Lsp1109I	GCAGC	3 cut(s)	110, 1468, 1558
LweI	GCATC	5 cut(s)	130, 286, 420, 824, 1626
MaeI	CTAG	1 cut(s)	1220
MaeII	ACGT	3 cut(s)	322, 620, 1098
MaeIII	GTNAC	5 cut(s)	542, 686, 712, 1126, 1456
MboII	GAAGA	4 cut(s)	509, 1199, 1290, 1631
MflI	RGATCY	3 cut(s)	160, 268, 820
MhlI	GDGCHC	3 cut(s)	1408, 1500, 1590
MluCI	AATT	9 cut(s)	196, 208, 250, 511, 585, 1079, 1140, 1255, 1465
MlyI	GAGTC	4 cut(s)	71, 128, 720, 976
MmeI	TCCRAC	2 cut(s)	194, 273
Mph1103I	ATGCAT	2 cut(s)	431, 739
MseI	TTAA	6 cut(s)	390, 627, 780, 858, 1044, 1176
MslI	CAYNNNNRTG	2 cut(s)	219, 350
MspI	CCGG	1 cut(s)	1516
MspR9I	CCNGG	1 cut(s)	151
Mva1269I	GAATGC	3 cut(s)	226, 769, 1511
MvaI	CCWGG	1 cut(s)	151
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	1 cut(s)	296
NcoI	CCATGG	2 cut(s)	952, 1250
NdeI	CATATG	1 cut(s)	1320
NlaIV	GGNNCC	2 cut(s)	994, 1236
NmuCI	GTSAC	3 cut(s)	542, 712, 1456
NsiI	ATGCAT	2 cut(s)	431, 739
NspI	RCATGY	2 cut(s)	278, 737
NspV	TTCGAA	1 cut(s)	383
PagI	TCATGA	1 cut(s)	189
PciSI	GCTCTTC	1 cut(s)	1614
PctI	GAATGC	3 cut(s)	226, 769, 1511
PfeI	GAWTC	4 cut(s)	26, 64, 1288, 1643
PkrI	GCNGC	3 cut(s)	125, 1483, 1573
PleI	GAGTC	4 cut(s)	71, 128, 719, 975
PpsI	GAGTC	4 cut(s)	71, 128, 719, 975
PsiI	TTATAA	4 cut(s)	371, 939, 1055, 1464
Psp6I	CCWGG	1 cut(s)	149
PspGI	CCWGG	1 cut(s)	149
PspN4I	GGNNCC	2 cut(s)	994, 1236
PspPI	GGNCC	3 cut(s)	590, 904, 1165
PsrI	GAACNNNNNNTAC	2 cut(s)	971, 1003
PstNI	CAGNNNCTG	1 cut(s)	1168
PsuI	RGATCY	3 cut(s)	160, 268, 820
RsaI	GTAC	2 cut(s)	732, 1297
RsaNI	GTAC	2 cut(s)	731, 1296
RseI	CAYNNNNRTG	2 cut(s)	219, 350
SapI	GCTCTTC	1 cut(s)	1614
SaqAI	TTAA	6 cut(s)	390, 627, 780, 858, 1044, 1176
SatI	GCNGC	3 cut(s)	124, 1482, 1572
Sau96I	GGNCC	3 cut(s)	590, 904, 1165
ScaI	AGTACT	1 cut(s)	1297
SchI	GAGTC	4 cut(s)	71, 128, 720, 976
ScrFI	CCNGG	1 cut(s)	151
SduI	GDGCHC	3 cut(s)	1408, 1500, 1590
SfaNI	GCATC	5 cut(s)	130, 286, 420, 824, 1626
SfuI	TTCGAA	1 cut(s)	383
SinI	GGWCC	1 cut(s)	904
SmiMI	CAYNNNNRTG	2 cut(s)	219, 350
Sse9I	AATT	9 cut(s)	196, 208, 250, 511, 585, 1079, 1140, 1255, 1465
SsiI	CCGC	3 cut(s)	21, 278, 1639
SspMI	CTAG	1 cut(s)	1220
StyD4I	CCNGG	1 cut(s)	149
StyI	CCWWGG	2 cut(s)	952, 1250
TaaI	ACNGT	4 cut(s)	314, 692, 874, 992
TaiI	ACGT	3 cut(s)	325, 623, 1101
TaqI	TCGA	5 cut(s)	67, 75, 383, 970, 1266
TasI	AATT	9 cut(s)	196, 208, 250, 511, 585, 1079, 1140, 1255, 1465
TatI	WGTACW	2 cut(s)	730, 1295
TfiI	GAWTC	4 cut(s)	26, 64, 1288, 1643
Tru1I	TTAA	6 cut(s)	390, 627, 780, 858, 1044, 1176
Tru9I	TTAA	6 cut(s)	390, 627, 780, 858, 1044, 1176
TscAI	CASTG	2 cut(s)	697, 1595
TseFI	GTSAC	3 cut(s)	542, 712, 1456
TseI	GCWGC	3 cut(s)	123, 1481, 1571
Tsp45I	GTSAC	3 cut(s)	542, 712, 1456
TspDTI	ATGAA	9 cut(s)	178, 875, 912, 926, 992, 1119, 1134, 1542, 1622
TspGWI	ACGGA	1 cut(s)	540
TspRI	CASTG	2 cut(s)	697, 1595
VpaK11BI	GGWCC	1 cut(s)	904
XapI	RAATTY	1 cut(s)	1140
XceI	RCATGY	2 cut(s)	278, 737
XmiI	GTMKAC	1 cut(s)	1352
XspI	CTAG	1 cut(s)	1220
ZrmI	AGTACT	1 cut(s)	1297
Zsp2I	ATGCAT	2 cut(s)	431, 739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.