Rh6CG528100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009978
Physical Location & Seq
Forward (+)
68206819 .. 68209430
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG528100.1

Sequence Viewer

Length: 1791 bp
ATGGCGTGTGCAATTGTTTGCGGAGAATCATCGCCGTCCCACATTACTCTTTTCCGATGCTTGAATCGAACCCTGGACTCTATCAAGGACAATATTGCTATCATCTTCGCTAACATTTTGGGCTGCCAAGCTAACACTGATGAAAATTTCAGGAATTGTAGGAGTGAGGATCTAGTTCCATCTAGTTTCGAGGAATTGCTGGAAAAATGGGGTGAGGATAACCACTTGATTGATCAGGATTGGTTTTCGAGGAAGAAGAATGCCGGCAATGATCAGGATCCGCAGGCAGAGTTCAATGTGTTGGATACAATGCTGAAGGATAGTTTGGAGCGTCTGAAAACAATGAGGGAAAGCATATCAGTGGCAAAGATAGGTTTTTTGGGATGCACTTTGAAAGACAATTACTCAGAACACATGGACATCATAAGGGGTTTATGCGTTGATGGTAAGTTGGGGTCAGCCTTTTGGCTTCGGAGAAAGATGATGAAGAAAGGGATTATTCCAGATGTATCGACACATAATTATCTATTGAATGGACTGTGTAAAGCTGGCGACTTGGAGAAGGCAAATTTGCTTATCAGGGAGATGCTAGAAATGGGTCCCGCTCCCAATTGTGTCACATACAACACTTTTATTAAGGGTTATTGCCTTCTTGATAAAGTGGATAAAGCTCTTTATCTTTTCTCCACAATGGGTAACAGTGGTATTAGGCCGAATAGTATTACTTGTAATATTCTTGTACATGCATTGTGCAAGAAAGGTCTTGCGGAGAATGCTAGGAAGCTTCTTGAAGAGATATTAGATGATGTTGATGATGAGAAAACAGACTCAGGTATAATCACCTTAACTACACTTATGGATGGTTATCTTAAAAATGGAAATATGGTTCAGGCACTTAGTCTTTGGGTTGACATCCAAAGGAATACCCAAGTAGATGTCATTGCTTATAATGTTCTCATCCGTGGATTTTGTTTGAGTCGGCAAATGAACCTTGCTTATGGATACTTCTGTGAAATGACCAAAAGAGGTTTACATCCTGATGTTTTTACTTGTAATACACTTATAAGTGGTCTTTGCAAAGAAAGAAAGTTTGAGGAGGCTTGTTACATCCATGGTATTATGCCAAGAATGGGAATTACTCCAGATAAAGTTTCATACAAAATAATTATACAAGGCCTATGTATCCTGGGAGATGTTGTCAAAGCTCATGAATTTCTTCTTAGTATGTTGGAGAAGTCGATGGTGCCTGAGCCTTTGATATGGAATTGTATTATTGATTGTTATGGAAGATATGGGGATCTTAGTAATGCAGTTTCTGTTAAGGATCGGATGTTTGCTTTTGGTGTTCAACCAAACGTATATACTTACAATGCATTGATCCACGCACAAGTGAAAGGAGGAAATATCATTGATGCTCTTTCTCTTGAAAAGGAAATGCTTCTGTCTGGTCTTTTTCCTGATGTGGTCACTTATAATATGTTGATAGGGGGAGCTTGTAACTCAGGGCATTTGGAATTTGCCACGGAGCTATATCATAAAATGCTGAAAATAGGACTTGATCCAGATATATATACTTACACTGAACTAATCAGAGGGAACTGCAAGAGAGGTAATATCAAGGAGGCAGAAAATGTTCTTGCGAAGATACAGGCATCTGGTTTGCCTATTGATGATGTTCCATTTCAGATACTTTTCAAGAAGTACTGCAAAATGAGGGAGCCTGATAAAGCATCTGGCATTTACGAGAAGTGGCTAGCGAGGAGGAATGAGTTGCCCTCTTTAAGAACTTAG

Protein Analysis

596

Amino Acids

67.14

Weight (kDa)

6.18

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 141 - 182 3.6e-09 PPR repeat family
PPR_3 PF13812 158 - 215 5.3e-08 Pentatricopeptide repeat domain
PPR_long PF17177 159 - 272 4.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 164 - 196 6.4e-09 PPR repeat
PPR_2 PF13041 170 - 216 2.5e-12 PPR repeat family
PPR_1 PF12854 200 - 231 1.5e-09 PPR repeat
PPR_2 PF13041 203 - 252 2.6e-15 PPR repeat family
PPR PF01535 206 - 236 7.7e-06 PPR repeat
PPR_1 PF12854 235 - 265 1.1e-06 PPR repeat
PPR_2 PF13041 312 - 360 5.8e-16 PPR repeat family
PPR_3 PF13812 338 - 391 9.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 342 - 368 1.3e-07 PPR repeat
PPR_2 PF13041 346 - 394 4.4e-15 PPR repeat family
PPR_2 PF13041 420 - 462 3.6e-10 PPR repeat family
PPR_long PF17177 429 - 571 5.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 439 - 496 2.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 451 - 500 1.5e-12 PPR repeat family
PPR_1 PF12854 483 - 514 3.4e-09 PPR repeat
PPR_2 PF13041 486 - 535 1.2e-15 PPR repeat family
PPR_1 PF12854 518 - 546 6.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 948, 1064, 1473
AccB1I GGYRCC 1 cut(s) 1243
AccBSI CCGCTC 1 cut(s) 605
AciI CCGC 4 cut(s) 21, 281, 603, 767
AclWI GGATC 7 cut(s) 177, 272, 285, 1305, 1332, 1372, 1553
AcsI RAATTY 4 cut(s) 145, 568, 1211, 1514
AcuI CTGAAG 1 cut(s) 335
AfaI GTAC 2 cut(s) 741, 1703
AfiI CCNNNNNNNGG 1 cut(s) 1130
AgsI TTSAA 8 cut(s) 64, 295, 394, 532, 791, 1349, 1427, 1696
AjnI CCWGG 2 cut(s) 72, 1185
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AloI GAACNNNNNNTCC 2 cut(s) 870, 902
AluBI AGCT 7 cut(s) 131, 548, 671, 784, 1205, 1493, 1528
AluI AGCT 7 cut(s) 131, 548, 671, 784, 1205, 1493, 1528
AlwI GGATC 7 cut(s) 177, 272, 285, 1305, 1332, 1372, 1553
AlwNI CAGNNNCTG 1 cut(s) 1316
AoxI GGCC 2 cut(s) 710, 1174
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 4 cut(s) 145, 568, 1211, 1514
Asp700I GAANNNNTTC 3 cut(s) 1215, 1437, 1632
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 224, 832
AsuNHI GCTAGC 1 cut(s) 1753
AvaII GGWCC 1 cut(s) 599
BaeI ACNNNNGTAYC 2 cut(s) 994, 1027
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 1243
BbvI GCAGC 1 cut(s) 110
BccI CCATC 4 cut(s) 187, 437, 854, 1234
BceAI ACGGC 1 cut(s) 19
BciT130I CCWGG 2 cut(s) 74, 1187
BciVI GTATCC 3 cut(s) 298, 995, 1193
BclI TGATCA 2 cut(s) 232, 271
BfaI CTAG 5 cut(s) 173, 183, 590, 777, 1754
BfuI GTATCC 3 cut(s) 298, 995, 1193
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
BmcAI AGTACT 1 cut(s) 1703
Bme1390I CCNGG 2 cut(s) 74, 1187
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 5 cut(s) 279, 600, 601, 1245, 1719
BmrFI CCNGG 2 cut(s) 74, 1187
BmsI GCATC 6 cut(s) 47, 374, 576, 1402, 1661, 1739
BmtI GCTAGC 1 cut(s) 1757
BplI GAGNNNNNCTC 1 cut(s) 1760
BpmI CTGGAG 1 cut(s) 1125
Bpu10I CCTNAGC 1 cut(s) 1248
BsaBI GATNNNNATC 2 cut(s) 276, 864
BsaJI CCNNGG 5 cut(s) 72, 961, 1111, 1186, 1521
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 1 cut(s) 1130
Bse118I RCCGGY 1 cut(s) 263
Bse3DI GCAATG 2 cut(s) 274, 939
Bse8I GATNNNNATC 2 cut(s) 276, 864
BseBI CCWGG 2 cut(s) 74, 1187
BseDI CCNNGG 5 cut(s) 72, 961, 1111, 1186, 1521
BseGI GGATG 7 cut(s) 389, 865, 912, 957, 1033, 1107, 1335
BseJI GATNNNNATC 2 cut(s) 276, 864
BseLI CCNNNNNNNGG 1 cut(s) 1130
BseMI GCAATG 2 cut(s) 274, 939
BseMII CTCAG 4 cut(s) 420, 843, 1239, 1515
BseRI GAGGAG 2 cut(s) 1109, 1774
BseXI GCAGC 1 cut(s) 110
BshFI GGCC 2 cut(s) 712, 1176
BshNI GGYRCC 1 cut(s) 1243
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 2 cut(s) 22, 585
BslI CCNNNNNNNGG 1 cut(s) 1130
BsmFI GGGAC 2 cut(s) 22, 585
BsmI GAATGC 2 cut(s) 265, 778
BsnI GGCC 2 cut(s) 712, 1176
Bsp1407I TGTACA 1 cut(s) 739
Bsp143I GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
Bsp19I CCATGG 1 cut(s) 1111
BspACI CCGC 4 cut(s) 21, 281, 603, 767
BspANI GGCC 2 cut(s) 712, 1176
BspCNI CTCAG 4 cut(s) 419, 842, 1240, 1514
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 5 cut(s) 279, 600, 601, 1245, 1719
BspOI GCTAGC 1 cut(s) 1757
BspPI GGATC 7 cut(s) 177, 272, 285, 1305, 1332, 1372, 1553
BspT107I GGYRCC 1 cut(s) 1243
BsrBI CCGCTC 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 274, 939
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 739
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 5 cut(s) 72, 961, 1111, 1186, 1521
BssMI GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
BssT1I CCWWGG 1 cut(s) 1111
Bst2UI CCWGG 2 cut(s) 74, 1187
Bst4CI ACNGT 2 cut(s) 540, 701
Bst6I CTCTTC 1 cut(s) 786
BstAUI TGTACA 1 cut(s) 739
BstC8I GCNNGC 4 cut(s) 265, 285, 550, 1755
BstDEI CTNAG 8 cut(s) 406, 829, 896, 1220, 1248, 1301, 1501, 1788
BstDSI CCRYGG 3 cut(s) 961, 1111, 1521
BstF5I GGATG 7 cut(s) 389, 865, 912, 957, 1033, 1107, 1335
BstKTI GATC 8 cut(s) 172, 235, 274, 280, 1300, 1327, 1380, 1561
BstMBI GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
BstMWI GCNNNNNNNGC 1 cut(s) 773
BstNI CCWGG 2 cut(s) 74, 1187
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 72, 1185
BstV1I GCAGC 1 cut(s) 110
BstX2I RGATCY 3 cut(s) 169, 277, 1297
BstYI RGATCY 3 cut(s) 169, 277, 1297
BsuI GTATCC 3 cut(s) 298, 995, 1193
BsuRI GGCC 2 cut(s) 712, 1176
BtgI CCRYGG 3 cut(s) 961, 1111, 1521
BtgZI GCGATG 1 cut(s) 15
BtsCI GGATG 7 cut(s) 389, 865, 912, 957, 1033, 1107, 1335
BtsIMutI CAGTG 4 cut(s) 135, 366, 706, 1578
Cac8I GCNNGC 4 cut(s) 265, 285, 550, 1755
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 1 cut(s) 1207
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 740, 1702
CviAII CATG 4 cut(s) 415, 743, 1112, 1208
CviQI GTAC 2 cut(s) 740, 1702
DdeI CTNAG 8 cut(s) 406, 829, 896, 1220, 1248, 1301, 1501, 1788
DpnI GATC 8 cut(s) 171, 234, 273, 279, 1299, 1326, 1379, 1560
DpnII GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
Eco130I CCWWGG 1 cut(s) 1111
Eco147I AGGCCT 1 cut(s) 1176
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 72, 1185
EcoT14I CCWWGG 1 cut(s) 1111
EcoT22I ATGCAT 2 cut(s) 748, 1375
ErhI CCWWGG 1 cut(s) 1111
FaeI CATG 4 cut(s) 418, 746, 1115, 1211
FaqI GGGAC 2 cut(s) 22, 585
FatI CATG 4 cut(s) 414, 742, 1111, 1207
FauI CCCGC 1 cut(s) 610
FbaI TGATCA 2 cut(s) 232, 271
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 7 cut(s) 396, 872, 899, 944, 1020, 1094, 1342
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 5 cut(s) 173, 183, 590, 777, 1754
GluI GCNGC 1 cut(s) 124
GsuI CTGGAG 1 cut(s) 1125
HaeIII GGCC 2 cut(s) 712, 1176
HapII CCGG 1 cut(s) 264
HgaI GACGC 1 cut(s) 320
Hin1II CATG 4 cut(s) 418, 746, 1115, 1211
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 26, 64, 77, 827, 976
HpaII CCGG 1 cut(s) 264
HphI GGTGA 2 cut(s) 224, 832
Hpy166II GTNNAC 2 cut(s) 910, 1031
Hpy188I TCNGA 7 cut(s) 56, 336, 409, 474, 1329, 1592, 1686
Hpy8I GTNNAC 2 cut(s) 910, 1031
HpyAV CCTTC 3 cut(s) 310, 556, 659
HpyCH4III ACNGT 2 cut(s) 540, 701
HpyCH4IV ACGT 1 cut(s) 1356
HpyCH4V TGCA 9 cut(s) 11, 387, 746, 753, 1077, 1310, 1373, 1602, 1707
HpyF10VI GCNNNNNNNGC 1 cut(s) 773
HpyF3I CTNAG 8 cut(s) 406, 829, 896, 1220, 1248, 1301, 1501, 1788
HpySE526I ACGT 1 cut(s) 1356
Hsp92II CATG 4 cut(s) 418, 746, 1115, 1211
KflI GGGWCCC 1 cut(s) 599
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 232, 271
Kzo9I GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
LmnI GCTCC 5 cut(s) 328, 610, 1490, 1525, 1717
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 6 cut(s) 47, 374, 576, 1402, 1661, 1739
MaeI CTAG 5 cut(s) 173, 183, 590, 777, 1754
MaeII ACGT 1 cut(s) 1356
MaeIII GTNAC 5 cut(s) 616, 695, 1103, 1465, 1496
MalI GATC 8 cut(s) 171, 234, 273, 279, 1299, 1326, 1379, 1560
MbiI CCGCTC 1 cut(s) 605
MboI GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
MboII GAAGA 8 cut(s) 97, 265, 268, 499, 803, 1208, 1299, 1654
MfeI CAATTG 2 cut(s) 12, 610
MflI RGATCY 3 cut(s) 169, 277, 1297
MlyI GAGTC 3 cut(s) 71, 821, 985
MmeI TCCRAC 2 cut(s) 282, 1209
Mph1103I ATGCAT 2 cut(s) 748, 1375
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 3 cut(s) 1215, 1437, 1632
MseI TTAA 5 cut(s) 636, 845, 870, 1320, 1781
MslI CAYNNNNRTG 2 cut(s) 359, 1038
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 2 cut(s) 74, 1187
MunI CAATTG 2 cut(s) 12, 610
Mva1269I GAATGC 2 cut(s) 265, 778
MvaI CCWGG 2 cut(s) 74, 1187
MwoI GCNNNNNNNGC 1 cut(s) 773
NaeI GCCGGC 1 cut(s) 265
NcoI CCATGG 1 cut(s) 1111
NdeII GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
NgoMIV GCCGGC 1 cut(s) 263
NheI GCTAGC 1 cut(s) 1753
NlaIII CATG 4 cut(s) 418, 746, 1115, 1211
NlaIV GGNNCC 5 cut(s) 279, 600, 601, 1245, 1719
NmuCI GTSAC 2 cut(s) 616, 1465
NsiI ATGCAT 2 cut(s) 748, 1375
NspI RCATGY 1 cut(s) 746
PagI TCATGA 1 cut(s) 1207
PceI AGGCCT 1 cut(s) 1176
PctI GAATGC 2 cut(s) 265, 778
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 3 cut(s) 1215, 1437, 1632
PfeI GAWTC 2 cut(s) 26, 64
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 3 cut(s) 71, 821, 984
PpsI GAGTC 3 cut(s) 71, 821, 984
PpuMI RGGWCCY 1 cut(s) 599
PsiI TTATAA 3 cut(s) 948, 1064, 1473
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 72, 1185
PspGI CCWGG 2 cut(s) 72, 1185
PspN4I GGNNCC 5 cut(s) 279, 600, 601, 1245, 1719
PspPI GGNCC 1 cut(s) 599
PspPPI RGGWCCY 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 3 cut(s) 169, 277, 1297
RsaI GTAC 2 cut(s) 741, 1703
RsaNI GTAC 2 cut(s) 740, 1702
RseI CAYNNNNRTG 2 cut(s) 359, 1038
SaqAI TTAA 5 cut(s) 636, 845, 870, 1320, 1781
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 8 cut(s) 169, 232, 271, 277, 1297, 1324, 1377, 1558
Sau96I GGNCC 1 cut(s) 599
ScaI AGTACT 1 cut(s) 1703
SchI GAGTC 3 cut(s) 71, 821, 985
ScrFI CCNGG 2 cut(s) 74, 1187
SfaNI GCATC 6 cut(s) 47, 374, 576, 1402, 1661, 1739
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 2 cut(s) 359, 1038
SseBI AGGCCT 1 cut(s) 1176
SsiI CCGC 4 cut(s) 21, 281, 603, 767
SspI AATATT 2 cut(s) 94, 733
SspMI CTAG 5 cut(s) 173, 183, 590, 777, 1754
StuI AGGCCT 1 cut(s) 1176
StyD4I CCNGG 2 cut(s) 72, 1185
StyI CCWWGG 1 cut(s) 1111
TaaI ACNGT 2 cut(s) 540, 701
TaiI ACGT 1 cut(s) 1359
TaqI TCGA 5 cut(s) 67, 189, 248, 512, 1238
TatI WGTACW 2 cut(s) 739, 1701
TfiI GAWTC 2 cut(s) 26, 64
Tru1I TTAA 5 cut(s) 636, 845, 870, 1320, 1781
Tru9I TTAA 5 cut(s) 636, 845, 870, 1320, 1781
TscAI CASTG 4 cut(s) 142, 366, 706, 1585
TseFI GTSAC 2 cut(s) 616, 1465
TseI GCWGC 1 cut(s) 123
Tsp45I GTSAC 2 cut(s) 616, 1465
TspDTI ATGAA 5 cut(s) 156, 500, 1001, 1143, 1224
TspGWI ACGGA 2 cut(s) 950, 1538
TspRI CASTG 4 cut(s) 142, 366, 706, 1585
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 4 cut(s) 145, 568, 1211, 1514
XceI RCATGY 1 cut(s) 746
XmnI GAANNNNTTC 3 cut(s) 1215, 1437, 1632
XspI CTAG 5 cut(s) 173, 183, 590, 777, 1754
ZrmI AGTACT 1 cut(s) 1703
Zsp2I ATGCAT 2 cut(s) 748, 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.