Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009978

Physical Location & Seq

Genomic Coordinates Reverse (-)

23707200 .. 23711817

Gene Structure 4618 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30660.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGCGTGTGCAATCGTGTGCGGAGAAGCGTCGCCGTCTTACGTCACGCTTTTCCGAAGCTTGAATCGAACCCTGGACTCTATCAAGGACAACATCGCTGTCATCTTCGCTAACATTTTGGCCTGCCAAGCTCACAGTGACGACAATTTCAGGAGTGAGGAAGTAGTTCCATCCAGTTTCGAGGAATTGATGGAGAAATATAAGCACTTGAGTGCTGGGGATTGGTTTTCGAAGAAGAGGAATGCCGGCAATGATCAAGATCCACAGGCAGAGTTCAATGTGTTGGATACAATGCTCAAGGATAACTTGGAGCGTCTTAAGACAATGAGGGAAAGCATATCAGTGGCAAAGATAGGTTTTCTGGGATGCACTTCGAAAATCAATTACTTAGAACACATGGACGTCTTAAGGGGTTTATGTGTTGATGGTAAATTGGGGTCAGCCTTTTGGCTGCGGAGAAAACTGATGAATAAAGGGATTATTCCGGATGTATGGGCACATAATTGTCTATTGAATGCACTGTGTAAAGCTGGCGACTTGGAGAAGGCAAATTGGCTTATCAGGGAGATGCTAGAAATGGGCCCCTCTCCCAACTGTGTCACATACAACACTTTTATTAAGGGTTATTGCCTTCTTGATAAAGTGGATAAAGCTCTTTATCTTTTCACCACAATGGTTAACAGTGGTATTAGGCCGAATAGTATTACTTATAATATTCTTGTACATGCATTGTGCAAGAAAGGTCTTGTGGAGGATGCTAACAAGCTTCTTGAAGAGATATTAGATGATGATGAAGAGATAACAGACTCAGGTATAGTAATTTCAACTACTCTTATGCATGGTCATCTTAAAAATGGAAATATGGTTCAGGCACTTAGTCTTTGGGTTGACATGCAGAGGAATACCCAAGTAGATGTCATTGCTTACAATGTTCTCATCCATGGATTTTGTTTGAATCAGCAAATGGACCTTGCGTATGGATACCTATCTGAAATGACCAAAAGAGGTTTACTTCCTGATGTTTTTACTTGTAACACACTTATAAGTGGTCTCTGCAAAGAAAGAAAGTTTGAGGATGCTTGTTACATCCATGATATTATGCCAAGAATGGGAATTATTCCAGATAAGGTCACATACAACATAATTATACAAGGCTTATGTATCGAGGGAGATGTTGTCAAAGCTCATGAATTTCTTCGTAGTATGTTAGAGAAGTCGATGGTTCCTGAGCCTTTGGTATGGAATAGTATTATTGATTGCTATGCAAGATATGGAGATCTTAGTAATGCAGTTTCTGTTAAGGATCAGATGTTAGCTTTTGGTGTTCAACCAAACGTATATACTTACAATGCAATGATCCACGCTCAAGTGAAAGGAGGAAGTTTTATCGGTGCTCTTTCTGTTGAAAAGAAAATGCTGCTGTCGGGTCTTTTTCCTGATGTGGTCACCTATAATATGTTGATTGGGGGTGCTTGTAACTCAGGGCATTTTGAATTTGCCATAGAGTTATATCATAAAATGCTGAAAATAGGAATCGATCCAGATATATTTACTTACACTGAACTAATCAGAGGGAACTGCATGAGAGGTAATATCAAGGAGGCAGAAAAGATTCTTGCTACGATACTGGCATCTGGTTTCCCTGTTAATCATGTTCCATTTAAGATACTTTTCAAGAAGTACTGCAAAATGAGGGAGCCTGATAAAGCGTCTGGCATTTACCAGAAGTGGCTAGCGAGGAGAAATGAATATCCCTCTTTAATAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

590

Amino Acids

66.35

Weight (kDa)

6.3

Isoelectric Point (pI)

36.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	153 - 209	1.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	158 - 190	2.9e-07	PPR repeat
PPR_2	PF13041	162 - 210	1.9e-13	PPR repeat family
PPR	PF01535	167 - 194	4.2e-06	PPR repeat
PPR_1	PF12854	193 - 226	6.1e-10	PPR repeat
PPR_2	PF13041	197 - 246	5.5e-17	PPR repeat family
PPR	PF01535	200 - 230	5e-06	PPR repeat
PPR_1	PF12854	229 - 259	1.6e-11	PPR repeat
PPR	PF01535	235 - 262	7.7e-06	PPR repeat
PPR_2	PF13041	305 - 353	1.3e-15	PPR repeat family
PPR_3	PF13812	329 - 384	2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	335 - 367	1.3e-07	PPR repeat
PPR_2	PF13041	339 - 387	5.2e-17	PPR repeat family
PPR_1	PF12854	370 - 402	3.3e-10	PPR repeat
PPR_2	PF13041	374 - 409	5.3e-07	PPR repeat family
PPR_2	PF13041	413 - 455	4.7e-11	PPR repeat family
PPR_3	PF13812	432 - 489	3.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	476 - 507	7.5e-09	PPR repeat
PPR_2	PF13041	479 - 527	8.2e-16	PPR repeat family
PPR	PF01535	482 - 512	2.5e-06	PPR repeat
PPR_2	PF13041	514 - 563	8.5e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24830
fragaria_vesca	FvH4_2g30660 FvH4_2g30660 FvH4_2g30660 FvH4_2g30660 FvH4_2g30660
malus_domestica	MD08G1133900.v1.1 MD15G1110300.v1.1
prunus_persica	Prupe.1G462500_v2.0.a1
pyrus_communis	pycom08g11270
rosa_chinensis	RchiOBHm_Chr6g0312241
rosa_laevigata	RLG00000010339
rosa_multiflora	Rmu_sc0010924.1_g000002 Rmu_ssc0000424.1_g000002
rosa_roxburghii	Rroxscaffold_7G00156920
rosa_rugosa	Rorug06G0394300
rosa_samantha	Rh6AG511300 Rh6BG522300 Rh6CG528100 Rh6DG513600
rosa_wichuraiana	Rw6G044470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	711, 1043
AatII	GACGTC	1 cut(s)	405
AccIII	TCCGGA	1 cut(s)	484
AciI	CCGC	2 cut(s)	21, 454
AclWI	GGATC	4 cut(s)	254, 1311, 1351, 1532
AcsI	RAATTY	2 cut(s)	1190, 1493
AcyI	GRCGYC	1 cut(s)	402
AfaI	GTAC	2 cut(s)	723, 1682
AfiI	CCNNNNNNNGG	1 cut(s)	1109
AflII	CTTAAG	2 cut(s)	317, 406
AjnI	CCWGG	1 cut(s)	72
AleI	CACNNNNGTG	1 cut(s)	210
AloI	GAACNNNNNNTCC	2 cut(s)	849, 881
AluBI	AGCT	7 cut(s)	60, 131, 530, 653, 766, 1184, 1316
AluI	AGCT	7 cut(s)	60, 131, 530, 653, 766, 1184, 1316
Alw21I	GWGCWC	1 cut(s)	1396
Alw26I	GTCTC	1 cut(s)	1055
AlwI	GGATC	4 cut(s)	254, 1311, 1351, 1532
AlwNI	CAGNNNCTG	1 cut(s)	1295
Aor13HI	TCCGGA	1 cut(s)	484
AoxI	GGCC	3 cut(s)	120, 580, 692
ApaI	GGGCCC	1 cut(s)	584
ApeKI	GCWGC	2 cut(s)	451, 1417
ApoI	RAATTY	2 cut(s)	1190, 1493
Asp700I	GAANNNNTTC	3 cut(s)	165, 1194, 1611
AspS9I	GGNCC	3 cut(s)	580, 581, 967
AsuHPI	GGTGA	2 cut(s)	658, 1438
AsuII	TTCGAA	2 cut(s)	230, 374
AsuNHI	GCTAGC	1 cut(s)	1732
AvaII	GGWCC	1 cut(s)	967
BaeGI	GKGCMC	2 cut(s)	499, 584
BanII	GRGCYC	1 cut(s)	584
Bbv12I	GWGCWC	1 cut(s)	1396
BbvI	GCAGC	2 cut(s)	438, 1404
BccI	CCATC	4 cut(s)	178, 184, 419, 1213
BceAI	ACGGC	1 cut(s)	19
BciT130I	CCWGG	1 cut(s)	74
BciVI	GTATCC	2 cut(s)	280, 974
BclI	TGATCA	1 cut(s)	253
BcoDI	GTCTC	1 cut(s)	1055
BfaI	CTAG	2 cut(s)	572, 1733
BfrI	CTTAAG	2 cut(s)	317, 406
BfuI	GTATCC	2 cut(s)	280, 974
BglII	AGATCT	1 cut(s)	1276
BisI	GCNGC	2 cut(s)	452, 1418
BlsI	GCNGC	2 cut(s)	453, 1419
BmcAI	AGTACT	1 cut(s)	1682
Bme1390I	CCNGG	1 cut(s)	74
Bme18I	GGWCC	1 cut(s)	967
BmgT120I	GGNCC	3 cut(s)	580, 581, 967
BmiI	GGNNCC	4 cut(s)	582, 583, 1224, 1698
BmrFI	CCNGG	1 cut(s)	74
BmsI	GCATC	5 cut(s)	356, 558, 745, 1066, 1640
BmtI	GCTAGC	1 cut(s)	1736
Bpu10I	CCTNAGC	1 cut(s)	1227
Bpu14I	TTCGAA	2 cut(s)	230, 374
BpuEI	CTTGAG	3 cut(s)	229, 281, 1350
Bsa29I	ATCGAT	1 cut(s)	1536
BsaBI	GATNNNNATC	2 cut(s)	258, 985
BsaHI	GRCGYC	1 cut(s)	402
BsaI	GGTCTC	1 cut(s)	1055
BsaJI	CCNNGG	2 cut(s)	72, 940
BsaWI	WCCGGW	1 cut(s)	484
BsaXI	ACNNNNNCTCC	2 cut(s)	1161, 1191
Bsc4I	CCNNNNNNNGG	1 cut(s)	1109
Bse118I	RCCGGY	1 cut(s)	245
Bse1I	ACTGG	2 cut(s)	174, 1632
Bse3DI	GCAATG	3 cut(s)	256, 918, 1359
Bse8I	GATNNNNATC	2 cut(s)	258, 985
BseAI	TCCGGA	1 cut(s)	484
BseBI	CCWGG	1 cut(s)	74
BseCI	ATCGAT	1 cut(s)	1536
BseDI	CCNNGG	2 cut(s)	72, 940
BseGI	GGATG	7 cut(s)	170, 371, 493, 760, 936, 1081, 1086
BseJI	GATNNNNATC	2 cut(s)	258, 985
BseLI	CCNNNNNNNGG	1 cut(s)	1109
BseMI	GCAATG	3 cut(s)	256, 918, 1359
BseMII	CTCAG	3 cut(s)	822, 1218, 1494
BseNI	ACTGG	2 cut(s)	174, 1632
BseRI	GAGGAG	1 cut(s)	1753
BseSI	GKGCMC	2 cut(s)	499, 584
BseXI	GCAGC	2 cut(s)	438, 1404
BseYI	CCCAGC	1 cut(s)	215
BshFI	GGCC	3 cut(s)	122, 582, 694
BshVI	ATCGAT	1 cut(s)	1536
BsiHKAI	GWGCWC	1 cut(s)	1396
BsiSI	CCGG	2 cut(s)	246, 485
BslI	CCNNNNNNNGG	1 cut(s)	1109
BsmAI	GTCTC	1 cut(s)	1055
BsmI	GAATGC	2 cut(s)	247, 520
BsnI	GGCC	3 cut(s)	122, 582, 694
Bso31I	GGTCTC	1 cut(s)	1055
Bsp119I	TTCGAA	2 cut(s)	230, 374
Bsp120I	GGGCCC	1 cut(s)	580
Bsp1286I	GDGCHC	3 cut(s)	499, 584, 1396
Bsp13I	TCCGGA	1 cut(s)	484
Bsp1407I	TGTACA	1 cut(s)	721
Bsp143I	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
Bsp19I	CCATGG	1 cut(s)	940
BspACI	CCGC	2 cut(s)	21, 454
BspANI	GGCC	3 cut(s)	122, 582, 694
BspCNI	CTCAG	3 cut(s)	821, 1219, 1493
BspDI	ATCGAT	1 cut(s)	1536
BspEI	TCCGGA	1 cut(s)	484
BspHI	TCATGA	1 cut(s)	1186
BspLI	GGNNCC	4 cut(s)	582, 583, 1224, 1698
BspOI	GCTAGC	1 cut(s)	1736
BspPI	GGATC	4 cut(s)	254, 1311, 1351, 1532
BspT104I	TTCGAA	2 cut(s)	230, 374
BspTI	CTTAAG	2 cut(s)	317, 406
BspTNI	GGTCTC	1 cut(s)	1055
BsrDI	GCAATG	3 cut(s)	256, 918, 1359
BsrFI	RCCGGY	1 cut(s)	245
BsrGI	TGTACA	1 cut(s)	721
BsrI	ACTGG	2 cut(s)	174, 1632
BssAI	RCCGGY	1 cut(s)	245
BssECI	CCNNGG	2 cut(s)	72, 940
BssMI	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
BssNI	GRCGYC	1 cut(s)	402
BssT1I	CCWWGG	1 cut(s)	940
Bst2UI	CCWGG	1 cut(s)	74
Bst4CI	ACNGT	4 cut(s)	137, 522, 596, 683
Bst6I	CTCTTC	3 cut(s)	230, 768, 789
BstACI	GRCGYC	1 cut(s)	402
BstAFI	CTTAAG	2 cut(s)	317, 406
BstAUI	TGTACA	1 cut(s)	721
BstBI	TTCGAA	2 cut(s)	230, 374
BstC8I	GCNNGC	4 cut(s)	124, 247, 532, 1734
BstDEI	CTNAG	7 cut(s)	388, 808, 875, 1227, 1280, 1480, 1767
BstDSI	CCRYGG	1 cut(s)	940
BstEII	GGTNACC	1 cut(s)	1444
BstF5I	GGATG	7 cut(s)	170, 371, 493, 760, 936, 1081, 1086
BstKTI	GATC	6 cut(s)	256, 262, 1279, 1306, 1359, 1540
BstMAI	GTCTC	1 cut(s)	1055
BstMBI	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
BstMWI	GCNNNNNNNGC	1 cut(s)	128
BstNI	CCWGG	1 cut(s)	74
BstNSI	RCATGY	2 cut(s)	728, 895
BstPI	GGTNACC	1 cut(s)	1444
BstSCI	CCNGG	1 cut(s)	72
BstSLI	GKGCMC	2 cut(s)	499, 584
BstV1I	GCAGC	2 cut(s)	438, 1404
BstX2I	RGATCY	2 cut(s)	259, 1276
BstYI	RGATCY	2 cut(s)	259, 1276
Bsu15I	ATCGAT	1 cut(s)	1536
BsuI	GTATCC	2 cut(s)	280, 974
BsuRI	GGCC	3 cut(s)	122, 582, 694
BsuTUI	ATCGAT	1 cut(s)	1536
BtgI	CCRYGG	1 cut(s)	940
BtgZI	GCGATG	1 cut(s)	79
BtsCI	GGATG	7 cut(s)	170, 371, 493, 760, 936, 1081, 1086
BtsIMutI	CAGTG	5 cut(s)	142, 348, 518, 688, 1557
Cac8I	GCNNGC	4 cut(s)	124, 247, 532, 1734
CaiI	CAGNNNCTG	1 cut(s)	1295
CciI	TCATGA	1 cut(s)	1186
Cfr10I	RCCGGY	1 cut(s)	245
Cfr13I	GGNCC	3 cut(s)	580, 581, 967
ClaI	ATCGAT	1 cut(s)	1536
CseI	GACGC	3 cut(s)	18, 302, 1698
Csp6I	GTAC	2 cut(s)	722, 1681
CviAII	CATG	9 cut(s)	397, 725, 839, 892, 941, 1091, 1187, 1582, 1652
CviQI	GTAC	2 cut(s)	722, 1681
DdeI	CTNAG	7 cut(s)	388, 808, 875, 1227, 1280, 1480, 1767
DpnI	GATC	6 cut(s)	255, 261, 1278, 1305, 1358, 1539
DpnII	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
Eam1104I	CTCTTC	3 cut(s)	230, 768, 789
EarI	CTCTTC	3 cut(s)	230, 768, 789
Eco130I	CCWWGG	1 cut(s)	940
Eco24I	GRGCYC	1 cut(s)	584
Eco31I	GGTCTC	1 cut(s)	1055
Eco47I	GGWCC	1 cut(s)	967
Eco91I	GGTNACC	1 cut(s)	1444
EcoO109I	RGGNCCY	1 cut(s)	581
EcoO65I	GGTNACC	1 cut(s)	1444
EcoRII	CCWGG	1 cut(s)	72
EcoT14I	CCWWGG	1 cut(s)	940
EcoT22I	ATGCAT	2 cut(s)	730, 840
EcoT38I	GRGCYC	1 cut(s)	584
ErhI	CCWWGG	1 cut(s)	940
FaeI	CATG	9 cut(s)	400, 728, 842, 895, 944, 1094, 1190, 1585, 1655
FalI	AAGNNNNNCTT	2 cut(s)	290, 322
FatI	CATG	9 cut(s)	396, 724, 838, 891, 940, 1090, 1186, 1581, 1651
FbaI	TGATCA	1 cut(s)	253
Fnu4HI	GCNGC	2 cut(s)	452, 1418
FokI	GGATG	7 cut(s)	157, 378, 500, 767, 923, 1073, 1088
FriOI	GRGCYC	1 cut(s)	584
Fsp4HI	GCNGC	2 cut(s)	452, 1418
FspBI	CTAG	2 cut(s)	572, 1733
GluI	GCNGC	2 cut(s)	452, 1418
GsaI	CCCAGC	1 cut(s)	219
HaeIII	GGCC	3 cut(s)	122, 582, 694
HapII	CCGG	2 cut(s)	246, 485
HgaI	GACGC	3 cut(s)	18, 302, 1698
Hin1I	GRCGYC	1 cut(s)	402
Hin1II	CATG	9 cut(s)	400, 728, 842, 895, 944, 1094, 1190, 1585, 1655
HincII	GTYRAC	2 cut(s)	679, 889
HindII	GTYRAC	2 cut(s)	679, 889
HindIII	AAGCTT	2 cut(s)	58, 764
HinfI	GANTC	6 cut(s)	64, 77, 806, 955, 1533, 1612
HpaI	GTTAAC	1 cut(s)	679
HpaII	CCGG	2 cut(s)	246, 485
HphI	GGTGA	2 cut(s)	658, 1438
Hpy166II	GTNNAC	3 cut(s)	679, 889, 1010
Hpy188I	TCNGA	4 cut(s)	56, 991, 1308, 1571
Hpy8I	GTNNAC	3 cut(s)	679, 889, 1010
Hpy99I	CGWCG	1 cut(s)	34
HpyAV	CCTTC	2 cut(s)	538, 641
HpyCH4III	ACNGT	4 cut(s)	137, 522, 596, 683
HpyCH4IV	ACGT	3 cut(s)	42, 402, 1335
HpyF10VI	GCNNNNNNNGC	1 cut(s)	128
HpyF3I	CTNAG	7 cut(s)	388, 808, 875, 1227, 1280, 1480, 1767
HpySE526I	ACGT	3 cut(s)	42, 402, 1335
Hsp92I	GRCGYC	1 cut(s)	402
Hsp92II	CATG	9 cut(s)	400, 728, 842, 895, 944, 1094, 1190, 1585, 1655
Kpn2I	TCCGGA	1 cut(s)	484
KroI	GCCGGC	1 cut(s)	245
KroNI	GCCGGC	1 cut(s)	247
Ksp22I	TGATCA	1 cut(s)	253
KspAI	GTTAAC	1 cut(s)	679
Kzo9I	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
LmnI	GCTCC	2 cut(s)	310, 1696
Lsp1109I	GCAGC	2 cut(s)	438, 1404
LweI	GCATC	5 cut(s)	356, 558, 745, 1066, 1640
MaeI	CTAG	2 cut(s)	572, 1733
MaeII	ACGT	3 cut(s)	42, 402, 1335
MaeIII	GTNAC	8 cut(s)	43, 137, 598, 1031, 1082, 1129, 1444, 1475
MalI	GATC	6 cut(s)	255, 261, 1278, 1305, 1358, 1539
MboI	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
MboII	GAAGA	6 cut(s)	97, 244, 247, 785, 806, 1187
MflI	RGATCY	2 cut(s)	259, 1276
MhlI	GDGCHC	3 cut(s)	499, 584, 1396
MlyI	GAGTC	2 cut(s)	71, 800
MmeI	TCCRAC	1 cut(s)	264
Mph1103I	ATGCAT	2 cut(s)	730, 840
MroI	TCCGGA	1 cut(s)	484
MroNI	GCCGGC	1 cut(s)	245
MroXI	GAANNNNTTC	3 cut(s)	165, 1194, 1611
MseI	TTAA	9 cut(s)	318, 407, 618, 678, 849, 1299, 1647, 1662, 1760
MslI	CAYNNNNRTG	3 cut(s)	210, 341, 671
MspCI	CTTAAG	2 cut(s)	317, 406
MspI	CCGG	2 cut(s)	246, 485
MspR9I	CCNGG	1 cut(s)	74
Mva1269I	GAATGC	2 cut(s)	247, 520
MvaI	CCWGG	1 cut(s)	74
MwoI	GCNNNNNNNGC	1 cut(s)	128
NaeI	GCCGGC	1 cut(s)	247
NcoI	CCATGG	1 cut(s)	940
NdeII	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
NgoMIV	GCCGGC	1 cut(s)	245
NheI	GCTAGC	1 cut(s)	1732
NlaIII	CATG	9 cut(s)	400, 728, 842, 895, 944, 1094, 1190, 1585, 1655
NlaIV	GGNNCC	4 cut(s)	582, 583, 1224, 1698
NmuCI	GTSAC	5 cut(s)	43, 137, 598, 1129, 1444
NsiI	ATGCAT	2 cut(s)	730, 840
NspI	RCATGY	2 cut(s)	728, 895
NspV	TTCGAA	2 cut(s)	230, 374
OliI	CACNNNNGTG	1 cut(s)	210
PagI	TCATGA	1 cut(s)	1186
PctI	GAATGC	2 cut(s)	247, 520
PdiI	GCCGGC	1 cut(s)	247
PdmI	GAANNNNTTC	3 cut(s)	165, 1194, 1611
PfeI	GAWTC	4 cut(s)	64, 955, 1533, 1612
PkrI	GCNGC	2 cut(s)	453, 1419
PleI	GAGTC	2 cut(s)	71, 800
PpsI	GAGTC	2 cut(s)	71, 800
PsiI	TTATAA	2 cut(s)	711, 1043
Psp6I	CCWGG	1 cut(s)	72
PspEI	GGTNACC	1 cut(s)	1444
PspFI	CCCAGC	1 cut(s)	215
PspGI	CCWGG	1 cut(s)	72
PspN4I	GGNNCC	4 cut(s)	582, 583, 1224, 1698
PspOMI	GGGCCC	1 cut(s)	580
PspPI	GGNCC	3 cut(s)	580, 581, 967
PstNI	CAGNNNCTG	1 cut(s)	1295
PsuI	RGATCY	2 cut(s)	259, 1276
RsaI	GTAC	2 cut(s)	723, 1682
RsaNI	GTAC	2 cut(s)	722, 1681
RseI	CAYNNNNRTG	3 cut(s)	210, 341, 671
SaqAI	TTAA	9 cut(s)	318, 407, 618, 678, 849, 1299, 1647, 1662, 1760
SatI	GCNGC	2 cut(s)	452, 1418
Sau3AI	GATC	6 cut(s)	253, 259, 1276, 1303, 1356, 1537
Sau96I	GGNCC	3 cut(s)	580, 581, 967
ScaI	AGTACT	1 cut(s)	1682
SchI	GAGTC	2 cut(s)	71, 800
ScrFI	CCNGG	1 cut(s)	74
SduI	GDGCHC	3 cut(s)	499, 584, 1396
SfaNI	GCATC	5 cut(s)	356, 558, 745, 1066, 1640
SfuI	TTCGAA	2 cut(s)	230, 374
SinI	GGWCC	1 cut(s)	967
SmiMI	CAYNNNNRTG	3 cut(s)	210, 341, 671
SmlI	CTYRAG	5 cut(s)	208, 296, 317, 406, 1365
SmoI	CTYRAG	5 cut(s)	208, 296, 317, 406, 1365
SsiI	CCGC	2 cut(s)	21, 454
SspI	AATATT	1 cut(s)	715
SspMI	CTAG	2 cut(s)	572, 1733
StyD4I	CCNGG	1 cut(s)	72
StyI	CCWWGG	1 cut(s)	940
TaaI	ACNGT	4 cut(s)	137, 522, 596, 683
TaiI	ACGT	3 cut(s)	45, 405, 1338
TaqI	TCGA	7 cut(s)	67, 180, 230, 374, 1164, 1217, 1536
TatI	WGTACW	2 cut(s)	721, 1680
TfiI	GAWTC	4 cut(s)	64, 955, 1533, 1612
Tru1I	TTAA	9 cut(s)	318, 407, 618, 678, 849, 1299, 1647, 1662, 1760
Tru9I	TTAA	9 cut(s)	318, 407, 618, 678, 849, 1299, 1647, 1662, 1760
TscAI	CASTG	5 cut(s)	142, 348, 525, 688, 1564
TseFI	GTSAC	5 cut(s)	43, 137, 598, 1129, 1444
TseI	GCWGC	2 cut(s)	451, 1417
Tsp45I	GTSAC	5 cut(s)	43, 137, 598, 1129, 1444
TspDTI	ATGAA	4 cut(s)	482, 807, 1203, 1761
TspRI	CASTG	5 cut(s)	142, 348, 525, 688, 1564
Vha464I	CTTAAG	2 cut(s)	317, 406
VpaK11BI	GGWCC	1 cut(s)	967
XapI	RAATTY	2 cut(s)	1190, 1493
XceI	RCATGY	2 cut(s)	728, 895
XmnI	GAANNNNTTC	3 cut(s)	165, 1194, 1611
XspI	CTAG	2 cut(s)	572, 1733
ZraI	GACGTC	1 cut(s)	403
ZrmI	AGTACT	1 cut(s)	1682
Zsp2I	ATGCAT	2 cut(s)	730, 840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.