AT5G25460

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009179
Physical Location & Seq
Forward (+)
8863224 .. 8865677
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25460.1

Sequence Viewer

Length: 1110 bp
ATGGAAGGCGTCACCGTCGTGTCTTTCTTCCTTCTTTTCATCGCCACCGCCATGGCCGCCAAGTCCACCGTCTCCTTCCGTGACGGCATGTTACCAAACGGAGACTTCGAGCTAGGACCAAAACCATCAGACATGAAAGGAACAGAAATACTAAACAAACTAGCAATACCAAACTGGGAAGTCACAGGATTCGTCGAATACATTAAATCAGGACATAAACAAGGAGACATGCTTCTCGTTGTTCCCGCCGGTAAATTCGCTGTAAGACTTGGGAACGAAGCATCGATCAAACAAAGACTTAAAGTGGTTAAAGGAATGTATTACTCACTCACTTTTAGTGCTGCTAGAACTTGTGCACAAGACGAGAGACTTAACATATCTGTAGCACCTGACTCCGGTGTGATTCCGATTCAGACGGTTTATAGTAGTAGTGGTTGGGATTTATATGCTTGGGCGTTTCAAGCTGAGAGTGATGTTGCTGAAGTTGTGATTCATAATCCTGGTGTTGAGGAAGATCCAGCTTGTGGTCCACTTATTGATGGTGTTGCTATGAGATCTCTTTACCCTCCTAGACCAACTAATAAGAACATTTTGAAAAACGGAGGATTTGAAGAAGGTCCATTAGTATTACCCGGCTCGACAACTGGAGTTTTGATCCCACCGTTTATAGAAGACGACCACTCTCCTTTACCTGGATGGATGGTGGAGTCTCTCAAAGCTGTCAAGTACGTAGACGTTGAACATTTCTCAGTCCCACAGGGTCGCAGAGCTATTGAGCTTGTAGCGGGTAAAGAGAGTGCCATCGCTCAAGTGGTTCGGACTGTCATTGGGAAGACTTACGTGCTGTCTTTTGCGGTTGGAGACGCCAACAATGCTTGCAAAGGATCAATGGTGGTTGAGGCTTTTGCAGGAAAAGATACACTTAAGGTCCCTTACGAGTCGAAAGGCACAGGAGGGTTTAAACGAGCTTCTATTCGATTTGTGGCGGTTTCGACCCGATCAAGAATTATGTTCTACAGCACTTTCTATGCCATGAGGAGCGATGATTTCTCGTCATTGTGTGGGCCTGTGATCGATGATGTCAAGCTTATAAGCGTTCGTAAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

369

Amino Acids

39.98

Weight (kDa)

6.91

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 29 - 185 2.4e-70 Protein of unknown function (DUF642)
DUF642 PF04862 197 - 363 1.5e-14 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32460 AT4G32460 AT4G32460 AT4G32460 AT5G11420 AT5G25460
fragaria_vesca FvH4_2g29680
malus_domestica MD08G1143200.v1.1 MD15G1120000.v1.1
prunus_persica Prupe.1G472800_v2.0.a1
pyrus_communis pycom08g12190 pycom15g10810
rosa_chinensis RchiOBHm_Chr6g0298661
rosa_laevigata RLG00000011495
rosa_multiflora Rmu_sc0008099.1_g000008
rosa_roxburghii Rroxscaffold_7G00169440
rosa_rugosa Rorug06G0281900 Rorug06G0282000
rosa_samantha Rh6AG393500 Rh6BG401300 Rh6DG393900
rosa_wichuraiana Rw6G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 524
AccI GTMKAC 1 cut(s) 732
AciI CCGC 6 cut(s) 48, 57, 246, 785, 854, 986
AclWI GGATC 3 cut(s) 509, 649, 892
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 2 cut(s) 9, 864
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 3 cut(s) 395, 524, 692
AflII CTTAAG 1 cut(s) 923
AgsI TTSAA 4 cut(s) 461, 595, 611, 740
AjnI CCWGG 2 cut(s) 499, 691
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 8 cut(s) 112, 464, 521, 719, 770, 778, 968, 1087
AluI AGCT 8 cut(s) 112, 464, 521, 719, 770, 778, 968, 1087
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 6 cut(s) 76, 96, 219, 361, 714, 855
Alw44I GTGCAC 1 cut(s) 354
AlwI GGATC 3 cut(s) 509, 649, 892
AoxI GGCC 2 cut(s) 54, 1064
ApaLI GTGCAC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 254
AspS9I GGNCC 5 cut(s) 116, 527, 617, 928, 1064
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 4 cut(s) 116, 527, 617, 928
BaeGI GKGCMC 1 cut(s) 358
BbsI GAAGAC 2 cut(s) 678, 839
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 328
BccI CCATC 5 cut(s) 133, 533, 690, 694, 809
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 2 cut(s) 501, 693
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 6 cut(s) 76, 96, 219, 361, 714, 855
BfaI CTAG 4 cut(s) 113, 161, 345, 570
BfmI CTRYAG 2 cut(s) 381, 1015
BfrI CTTAAG 1 cut(s) 923
BglII AGATCT 1 cut(s) 554
BisI GCNGC 2 cut(s) 57, 342
BlsI GCNGC 2 cut(s) 58, 343
Bme1390I CCNGG 3 cut(s) 501, 633, 693
Bme18I GGWCC 4 cut(s) 116, 527, 617, 928
BmgT120I GGNCC 5 cut(s) 116, 527, 617, 928, 1064
BmiI GGNNCC 1 cut(s) 930
BmrFI CCNGG 3 cut(s) 501, 633, 693
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 290
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 678, 839
BpmI CTGGAG 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 792
BpuMI CCSGG 1 cut(s) 633
Bsa29I ATCGAT 2 cut(s) 284, 1074
BsaAI YACGTR 2 cut(s) 730, 841
BsaHI GRCGYC 2 cut(s) 9, 864
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 395
Bsc4I CCNNNNNNNGG 3 cut(s) 395, 524, 692
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 179, 649
BseBI CCWGG 2 cut(s) 501, 693
BseCI ATCGAT 2 cut(s) 284, 1074
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 2 cut(s) 701, 705
BseLI CCNNNNNNNGG 3 cut(s) 395, 524, 692
BseMII CTCAG 2 cut(s) 456, 762
BseNI ACTGG 2 cut(s) 179, 649
BseRI GAGGAG 1 cut(s) 1051
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 1 cut(s) 328
BshFI GGCC 2 cut(s) 56, 1066
BshVI ATCGAT 2 cut(s) 284, 1074
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 3 cut(s) 249, 396, 633
BslFI GGGAC 2 cut(s) 737, 914
BslI CCNNNNNNNGG 3 cut(s) 395, 524, 692
BsmAI GTCTC 6 cut(s) 76, 96, 219, 361, 714, 855
BsmBI CGTCTC 2 cut(s) 76, 855
BsmFI GGGAC 2 cut(s) 737, 914
BsnI GGCC 2 cut(s) 56, 1066
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 6 cut(s) 48, 57, 246, 785, 854, 986
BspANI GGCC 2 cut(s) 56, 1066
BspCNI CTCAG 2 cut(s) 457, 761
BspDI ATCGAT 2 cut(s) 284, 1074
BspLI GGNNCC 1 cut(s) 930
BspPI GGATC 3 cut(s) 509, 649, 892
BspTI CTTAAG 1 cut(s) 923
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 2 cut(s) 179, 649
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
BssNI GRCGYC 2 cut(s) 9, 864
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 501, 693
Bst4CI ACNGT 5 cut(s) 16, 70, 418, 663, 823
BstACI GRCGYC 2 cut(s) 9, 864
BstAFI CTTAAG 1 cut(s) 923
BstBAI YACGTR 2 cut(s) 730, 841
BstC8I GCNNGC 1 cut(s) 877
BstDEI CTNAG 2 cut(s) 465, 748
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 701, 705
BstKTI GATC 7 cut(s) 288, 517, 557, 657, 887, 1001, 1074
BstMAI GTCTC 6 cut(s) 76, 96, 219, 361, 714, 855
BstMBI GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
BstMWI GCNNNNNNNGC 3 cut(s) 56, 461, 872
BstNI CCWGG 2 cut(s) 501, 693
BstNSI RCATGY 2 cut(s) 91, 232
BstSCI CCNGG 3 cut(s) 499, 631, 691
BstSFI CTRYAG 2 cut(s) 381, 1015
BstSLI GKGCMC 1 cut(s) 358
BstSNI TACGTA 1 cut(s) 730
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 2 cut(s) 678, 839
BstX2I RGATCY 2 cut(s) 514, 554
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 2 cut(s) 514, 554
Bsu15I ATCGAT 2 cut(s) 284, 1074
BsuRI GGCC 2 cut(s) 56, 1066
BsuTUI ATCGAT 2 cut(s) 284, 1074
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 3 cut(s) 25, 787, 1056
BtsCI GGATG 2 cut(s) 701, 705
Cac8I GCNNGC 1 cut(s) 877
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 5 cut(s) 116, 527, 617, 928, 1064
ClaI ATCGAT 2 cut(s) 284, 1074
CseI GACGC 1 cut(s) 872
Csp6I GTAC 1 cut(s) 727
CviAII CATG 5 cut(s) 52, 88, 133, 229, 1033
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 2 cut(s) 465, 748
DpnI GATC 7 cut(s) 287, 516, 556, 656, 886, 1000, 1073
DpnII GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
DraI TTTAAA 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 54
Eco105I TACGTA 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 51
Eco47I GGWCC 4 cut(s) 116, 527, 617, 928
Eco57I CTGAAG 1 cut(s) 501
EcoO109I RGGNCCY 1 cut(s) 928
EcoRII CCWGG 2 cut(s) 499, 691
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 2 cut(s) 76, 855
FaeI CATG 5 cut(s) 55, 91, 136, 232, 1036
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 2 cut(s) 737, 914
FatI CATG 5 cut(s) 51, 87, 132, 228, 1032
FauI CCCGC 2 cut(s) 253, 778
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 2 cut(s) 57, 342
FokI GGATG 2 cut(s) 708, 712
Fsp4HI GCNGC 2 cut(s) 57, 342
FspBI CTAG 4 cut(s) 113, 161, 345, 570
GluI GCNGC 2 cut(s) 57, 342
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 2 cut(s) 56, 1066
HapII CCGG 3 cut(s) 249, 396, 633
HgaI GACGC 1 cut(s) 872
Hin1I GRCGYC 2 cut(s) 9, 864
Hin1II CATG 5 cut(s) 55, 91, 136, 232, 1036
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 7 cut(s) 189, 392, 403, 409, 490, 707, 938
HpaII CCGG 3 cut(s) 249, 396, 633
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 5 cut(s) 66, 356, 530, 733, 1103
Hpy188I TCNGA 4 cut(s) 130, 408, 414, 819
Hpy188III TCNNGA 2 cut(s) 210, 1002
Hpy8I GTNNAC 5 cut(s) 66, 356, 530, 733, 1103
Hpy99I CGWCG 2 cut(s) 20, 197
HpyAV CCTTC 3 cut(s) 41, 85, 608
HpyCH4III ACNGT 5 cut(s) 16, 70, 418, 663, 823
HpyCH4IV ACGT 3 cut(s) 729, 735, 840
HpyCH4V TGCA 3 cut(s) 356, 879, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 461, 872
HpyF3I CTNAG 2 cut(s) 465, 748
HpySE526I ACGT 3 cut(s) 729, 735, 840
Hsp92I GRCGYC 2 cut(s) 9, 864
Hsp92II CATG 5 cut(s) 55, 91, 136, 232, 1036
Kzo9I GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
LmnI GCTCC 1 cut(s) 1038
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 1 cut(s) 290
MaeI CTAG 4 cut(s) 113, 161, 345, 570
MaeII ACGT 3 cut(s) 729, 735, 840
MaeIII GTNAC 4 cut(s) 10, 80, 90, 181
MalI GATC 7 cut(s) 287, 516, 556, 656, 886, 1000, 1073
MboI GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
MboII GAAGA 5 cut(s) 19, 524, 623, 683, 844
MflI RGATCY 2 cut(s) 514, 554
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 2 cut(s) 254, 1005
MlyI GAGTC 3 cut(s) 386, 716, 947
MmeI TCCRAC 1 cut(s) 838
MnlI CCTC 6 cut(s) 502, 576, 596, 892, 947, 1029
MseI TTAA 6 cut(s) 204, 300, 309, 372, 924, 960
MslI CAYNNNNRTG 2 cut(s) 17, 50
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 3 cut(s) 249, 396, 633
MspR9I CCNGG 3 cut(s) 501, 633, 693
MssI GTTTAAAC 1 cut(s) 961
MvaI CCWGG 2 cut(s) 501, 693
MwoI GCNNNNNNNGC 3 cut(s) 56, 461, 872
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 51
NdeII GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
NlaIII CATG 5 cut(s) 55, 91, 136, 232, 1036
NlaIV GGNNCC 1 cut(s) 930
NmuCI GTSAC 3 cut(s) 10, 80, 181
NspI RCATGY 2 cut(s) 91, 232
OliI CACNNNNGTG 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 4 cut(s) 189, 403, 409, 490
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 2 cut(s) 58, 343
PleI GAGTC 3 cut(s) 386, 715, 946
PmeI GTTTAAAC 1 cut(s) 961
PpsI GAGTC 3 cut(s) 386, 715, 946
Ppu21I YACGTR 2 cut(s) 730, 841
PpuMI RGGWCCY 1 cut(s) 928
PsiI TTATAA 1 cut(s) 1091
Psp5II RGGWCCY 1 cut(s) 928
Psp6I CCWGG 2 cut(s) 499, 691
PspGI CCWGG 2 cut(s) 499, 691
PspN4I GGNNCC 1 cut(s) 930
PspPI GGNCC 5 cut(s) 116, 527, 617, 928, 1064
PspPPI RGGWCCY 1 cut(s) 928
PsuI RGATCY 2 cut(s) 514, 554
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
RseI CAYNNNNRTG 2 cut(s) 17, 50
SaqAI TTAA 6 cut(s) 204, 300, 309, 372, 924, 960
SatI GCNGC 2 cut(s) 57, 342
Sau3AI GATC 7 cut(s) 285, 514, 554, 654, 884, 998, 1071
Sau96I GGNCC 5 cut(s) 116, 527, 617, 928, 1064
SchI GAGTC 3 cut(s) 386, 716, 947
ScrFI CCNGG 3 cut(s) 501, 633, 693
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 1 cut(s) 290
SfcI CTRYAG 2 cut(s) 381, 1015
SinI GGWCC 4 cut(s) 116, 527, 617, 928
SmiMI CAYNNNNRTG 2 cut(s) 17, 50
SmlI CTYRAG 2 cut(s) 807, 923
SmoI CTYRAG 2 cut(s) 807, 923
SnaBI TACGTA 1 cut(s) 730
Sse9I AATT 2 cut(s) 254, 1005
SsiI CCGC 6 cut(s) 48, 57, 246, 785, 854, 986
SspMI CTAG 4 cut(s) 113, 161, 345, 570
StyD4I CCNGG 3 cut(s) 499, 631, 691
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 5 cut(s) 16, 70, 418, 663, 823
TaiI ACGT 3 cut(s) 732, 738, 843
TaqI TCGA 8 cut(s) 108, 195, 284, 638, 941, 976, 992, 1074
TasI AATT 2 cut(s) 254, 1005
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 4 cut(s) 189, 403, 409, 490
Tru1I TTAA 6 cut(s) 204, 300, 309, 372, 924, 960
Tru9I TTAA 6 cut(s) 204, 300, 309, 372, 924, 960
TseFI GTSAC 3 cut(s) 10, 80, 181
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 3 cut(s) 10, 80, 181
TspDTI ATGAA 3 cut(s) 28, 149, 482
TspGWI ACGGA 3 cut(s) 68, 114, 615
Van91I CCANNNNNTGG 1 cut(s) 524
Vha464I CTTAAG 1 cut(s) 923
VneI GTGCAC 1 cut(s) 354
VpaK11BI GGWCC 4 cut(s) 116, 527, 617, 928
XapI RAATTY 1 cut(s) 254
XceI RCATGY 2 cut(s) 91, 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmiI GTMKAC 1 cut(s) 732
XspI CTAG 4 cut(s) 113, 161, 345, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.