MD15G1120000.v1.1

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009179
Physical Location & Seq
Forward (+)
8642522 .. 8647178
4657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1120000.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAGAGGGGTAGCCGTTTTGTTGTCGGTGCTGTTGTTTGCCACTTGCCACTCAGCCTTCTCCCTCATCGATGGTTACTTAGAAAATGGCAATTTTGAGCTACCCCCTAAGCCCTCAGACATAAAGGGTTCAGAAGTGATCGGCCGATATGCCATACCAAAGTGGGAGATTTCTGGGTTTGTGGAATACATTAAGTCAGGCCAAAAGCAAGGGGACATGTTGCTGGTTGTCCCCGAGGGAGCCTACGCAGTTAGGCTCGGAAATGAGGCCTCGATTAAGCAGAGCATTAAAGTGACTAAGGGGTTGTACTACTCAATCACTTTTAGTGCAGCGAGGACTTGTGCTCAAGAGGAACGTTTGAACGTTTCTGTGGCACCGGACTCCGGAGTGCTGCCTATCCAAACAGTGTATAGCAGCAACGGGTGGGATTCATATGCTTGGGCGTTTCAGGCGGATTATGAAACAGTTGAGCTTGTTTTGCATAACCCCGGAGTCGAGGAGGATCCGGCTTGCGGGCCTCTGATCGATTCGGTCGCCATCCGAACTTTGTACCCTCCTCGAGCAACCAACAAGAACTTGTTAAAAAACGCTGGATTTGAAGAAGGACCATACGTGTTCCCCAACACATCTTGGGGAGTCTTGATCCCACCAAACATAGAAGATGATCACTCCCCGTTACCAGGCTGGATGGTGGAGTCCCTCAAAGCAGTGAAGTACATAGACTCAGACCACTTCTCAGTCCCAGAAGGCAAAAGAGCGGTCGAACTTGTGGCCGGAAAAGAAAGCGCGATCGCCCAAGTCGTTCGAACCATCCCCGGCAAGACCTATGTGCTCTCATTCTCCGTAGGAGATGCAAGCAACTCATGCGAAGGCTCCATGATTGTGGAGGCATTTGCAGACAAGAACACGGTGAAAGTGCCTTACGAGTCCAAAGGCAAAGGCGGGTTCAAGCGGGCGGTGCTCAAATTCGTGGCGGCTTCGACCCGGACACGTGTCATGTTTCTAAGCACATATTACACCATGAGGAGTGATGACTTCTCTTCTCTGTGTGGACCTGTGGTTGATGACGTGAAGCTGTTGAGCCTTCGGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

366

Amino Acids

39.83

Weight (kDa)

5.73

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 25 - 181 5.7e-73 Protein of unknown function (DUF642)
DUF642 PF04862 192 - 359 6.7e-17 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32460 AT4G32460 AT4G32460 AT4G32460 AT5G11420 AT5G25460
fragaria_vesca FvH4_2g29680
malus_domestica MD08G1143200.v1.1 MD15G1120000.v1.1
prunus_persica Prupe.1G472800_v2.0.a1
pyrus_communis pycom08g12190 pycom15g10810
rosa_chinensis RchiOBHm_Chr6g0298661
rosa_laevigata RLG00000011495
rosa_multiflora Rmu_sc0008099.1_g000008
rosa_roxburghii Rroxscaffold_7G00169440
rosa_rugosa Rorug06G0281900 Rorug06G0282000
rosa_samantha Rh6AG393500 Rh6BG401300 Rh6DG393900
rosa_wichuraiana Rw6G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 758
AccII CGCG 1 cut(s) 788
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 7 cut(s) 452, 513, 758, 942, 952, 956, 974
AclI AACGTT 2 cut(s) 355, 363
AclWI GGATC 3 cut(s) 497, 510, 637
AcoI YGGCCR 2 cut(s) 142, 771
AcsI RAATTY 1 cut(s) 965
AcvI CACGTG 1 cut(s) 992
AfaI GTAC 3 cut(s) 308, 551, 716
AfiI CCNNNNNNNGG 3 cut(s) 383, 512, 680
AflIII ACRYGT 4 cut(s) 216, 612, 989, 991
AgsI TTSAA 3 cut(s) 361, 599, 949
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 3 cut(s) 100, 472, 1075
AluI AGCT 3 cut(s) 100, 472, 1075
Alw21I GWGCWC 3 cut(s) 346, 834, 963
AlwI GGATC 3 cut(s) 497, 510, 637
Ama87I CYCGRG 2 cut(s) 233, 558
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 5 cut(s) 142, 199, 267, 515, 771
ApeKI GCWGC 3 cut(s) 329, 391, 414
ApoI RAATTY 1 cut(s) 965
ArsI GACNNNNNNTTYG 2 cut(s) 744, 776
AsiSI GCGATCGC 1 cut(s) 792
AspLEI GCGC 1 cut(s) 788
AspS9I GGNCC 3 cut(s) 515, 605, 1052
AsuC2I CCSGG 3 cut(s) 489, 816, 985
AsuHPI GGTGA 1 cut(s) 922
AsuII TTCGAA 1 cut(s) 805
AvaI CYCGRG 2 cut(s) 233, 558
AvaII GGWCC 2 cut(s) 605, 1052
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 992
Bbv12I GWGCWC 3 cut(s) 346, 834, 963
BbvI GCAGC 3 cut(s) 341, 378, 426
BccI CCATC 4 cut(s) 65, 545, 682, 818
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 3 cut(s) 489, 816, 985
BfaI CTAG 1 cut(s) 1096
BisI GCNGC 4 cut(s) 330, 392, 415, 975
BlsI GCNGC 4 cut(s) 331, 393, 416, 976
Bme1390I CCNGG 4 cut(s) 489, 681, 816, 985
Bme18I GGWCC 2 cut(s) 605, 1052
BmeT110I CYCGRG 2 cut(s) 233, 558
BmgBI CACGTC 1 cut(s) 1069
BmgT120I GGNCC 3 cut(s) 515, 605, 1052
BmiI GGNNCC 4 cut(s) 241, 375, 504, 874
BmrFI CCNGG 4 cut(s) 489, 681, 816, 985
BmsI GCATC 1 cut(s) 841
BoxI GACNNNNGTC 1 cut(s) 992
Bpu10I CCTNAGC 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 3 cut(s) 489, 816, 985
Bsa29I ATCGAT 2 cut(s) 69, 525
BsaAI YACGTR 2 cut(s) 613, 992
BsaJI CCNNGG 3 cut(s) 234, 487, 814
BsaWI WCCGGW 2 cut(s) 376, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 383, 512, 680
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 1 cut(s) 681
BseCI ATCGAT 2 cut(s) 69, 525
BseDI CCNNGG 3 cut(s) 234, 487, 814
BseGI GGATG 3 cut(s) 537, 693, 810
BseLI CCNNNNNNNGG 3 cut(s) 383, 512, 680
BseMII CTCAG 4 cut(s) 66, 129, 738, 750
BseRI GAGGAG 3 cut(s) 512, 546, 1039
BseX3I CGGCCG 1 cut(s) 142
BseXI GCAGC 3 cut(s) 341, 378, 426
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 788
Bsh1285I CGRYCG 4 cut(s) 145, 534, 762, 792
BshFI GGCC 5 cut(s) 144, 201, 269, 517, 773
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 2 cut(s) 69, 525
BsiEI CGRYCG 4 cut(s) 145, 534, 762, 792
BsiHKAI GWGCWC 3 cut(s) 346, 834, 963
BsiHKCI CYCGRG 2 cut(s) 233, 558
BsiSI CCGG 7 cut(s) 377, 384, 489, 506, 774, 816, 985
BslFI GGGAC 4 cut(s) 215, 227, 682, 725
BslI CCNNNNNNNGG 3 cut(s) 383, 512, 680
BsmFI GGGAC 4 cut(s) 215, 227, 682, 725
BsnI GGCC 5 cut(s) 144, 201, 269, 517, 773
BsoBI CYCGRG 2 cut(s) 233, 558
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 346, 834, 963
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 6 cut(s) 138, 502, 522, 642, 664, 789
BspACI CCGC 7 cut(s) 452, 513, 758, 942, 952, 956, 974
BspANI GGCC 5 cut(s) 144, 201, 269, 517, 773
BspCNI CTCAG 4 cut(s) 65, 128, 737, 749
BspDI ATCGAT 2 cut(s) 69, 525
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 1 cut(s) 788
BspLI GGNNCC 4 cut(s) 241, 375, 504, 874
BspPI GGATC 3 cut(s) 497, 510, 637
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 758
BssECI CCNNGG 3 cut(s) 234, 487, 814
BssMI GATC 6 cut(s) 138, 502, 522, 642, 664, 789
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 3 cut(s) 406, 466, 910
Bst6I CTCTTC 1 cut(s) 1045
BstAPI GCANNNNNTGC 1 cut(s) 864
BstBAI YACGTR 2 cut(s) 613, 992
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 4 cut(s) 511, 515, 856, 954
BstDEI CTNAG 8 cut(s) 52, 79, 108, 115, 297, 724, 736, 1004
BstF5I GGATG 3 cut(s) 537, 693, 810
BstFNI CGCG 1 cut(s) 788
BstHHI GCGC 1 cut(s) 788
BstKTI GATC 6 cut(s) 141, 505, 525, 645, 667, 792
BstMBI GATC 6 cut(s) 138, 502, 522, 642, 664, 789
BstMCI CGRYCG 4 cut(s) 145, 534, 762, 792
BstMWI GCNNNNNNNGC 4 cut(s) 449, 478, 864, 958
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 992
BstSCI CCNGG 4 cut(s) 487, 679, 814, 983
BstUI CGCG 1 cut(s) 788
BstV1I GCAGC 3 cut(s) 341, 378, 426
BstX2I RGATCY 1 cut(s) 502
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 1 cut(s) 502
BstZI CGGCCG 1 cut(s) 142
Bsu15I ATCGAT 2 cut(s) 69, 525
BsuRI GGCC 5 cut(s) 144, 201, 269, 517, 773
BsuTUI ATCGAT 2 cut(s) 69, 525
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 3 cut(s) 537, 693, 810
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 411, 714
Cac8I GCNNGC 4 cut(s) 511, 515, 856, 954
CfoI GCGC 1 cut(s) 788
Cfr13I GGNCC 3 cut(s) 515, 605, 1052
ClaI ATCGAT 2 cut(s) 69, 525
Csp6I GTAC 3 cut(s) 307, 550, 715
CviAII CATG 5 cut(s) 217, 864, 877, 997, 1021
CviQI GTAC 3 cut(s) 307, 550, 715
DdeI CTNAG 8 cut(s) 52, 79, 108, 115, 297, 724, 736, 1004
DpnI GATC 6 cut(s) 140, 504, 524, 644, 666, 791
DpnII GATC 6 cut(s) 138, 502, 522, 642, 664, 789
EaeI YGGCCR 2 cut(s) 142, 771
EagI CGGCCG 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
EciI GGCGGA 1 cut(s) 467
EclXI CGGCCG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 605, 1052
Eco52I CGGCCG 1 cut(s) 142
Eco72I CACGTG 1 cut(s) 992
Eco88I CYCGRG 2 cut(s) 233, 558
EcoRII CCWGG 1 cut(s) 679
FaeI CATG 5 cut(s) 220, 867, 880, 1000, 1024
FaqI GGGAC 4 cut(s) 215, 227, 682, 725
FatI CATG 5 cut(s) 216, 863, 876, 996, 1020
FauI CCCGC 3 cut(s) 506, 935, 945
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 4 cut(s) 330, 392, 415, 975
FokI GGATG 3 cut(s) 524, 700, 797
Fsp4HI GCNGC 4 cut(s) 330, 392, 415, 975
FspBI CTAG 1 cut(s) 1096
GlaI GCGC 1 cut(s) 787
GluI GCNGC 4 cut(s) 330, 392, 415, 975
HaeIII GGCC 5 cut(s) 144, 201, 269, 517, 773
HapII CCGG 7 cut(s) 377, 384, 489, 506, 774, 816, 985
HhaI GCGC 1 cut(s) 788
Hin1II CATG 5 cut(s) 220, 867, 880, 1000, 1024
Hin6I GCGC 1 cut(s) 786
HinP1I GCGC 1 cut(s) 786
HinfI GANTC 8 cut(s) 380, 428, 492, 527, 636, 695, 722, 926
HpaII CCGG 7 cut(s) 377, 384, 489, 506, 774, 816, 985
HphI GGTGA 1 cut(s) 922
Hpy166II GTNNAC 1 cut(s) 1052
Hpy188I TCNGA 7 cut(s) 118, 133, 260, 522, 542, 727, 1089
Hpy188III TCNNGA 3 cut(s) 347, 384, 640
Hpy8I GTNNAC 1 cut(s) 1052
HpyAV CCTTC 5 cut(s) 67, 596, 740, 863, 1094
HpyCH4III ACNGT 3 cut(s) 406, 466, 910
HpyCH4IV ACGT 5 cut(s) 355, 363, 612, 991, 1068
HpyCH4V TGCA 4 cut(s) 329, 481, 854, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 449, 478, 864, 958
HpyF3I CTNAG 8 cut(s) 52, 79, 108, 115, 297, 724, 736, 1004
HpySE526I ACGT 5 cut(s) 355, 363, 612, 991, 1068
Hsp92II CATG 5 cut(s) 220, 867, 880, 1000, 1024
HspAI GCGC 1 cut(s) 786
Kpn2I TCCGGA 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 138, 502, 522, 642, 664, 789
LmnI GCTCC 2 cut(s) 239, 878
Lsp1109I GCAGC 3 cut(s) 341, 378, 426
LweI GCATC 1 cut(s) 841
MaeI CTAG 1 cut(s) 1096
MaeII ACGT 5 cut(s) 355, 363, 612, 991, 1068
MaeIII GTNAC 3 cut(s) 74, 292, 675
MalI GATC 6 cut(s) 140, 504, 524, 644, 666, 791
MbiI CCGCTC 1 cut(s) 758
MboI GATC 6 cut(s) 138, 502, 522, 642, 664, 789
MboII GAAGA 3 cut(s) 611, 671, 1032
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 3 cut(s) 346, 834, 963
MluCI AATT 2 cut(s) 91, 965
MlyI GAGTC 6 cut(s) 374, 501, 645, 704, 716, 935
MroI TCCGGA 1 cut(s) 383
MseI TTAA 4 cut(s) 192, 276, 288, 581
MslI CAYNNNNRTG 2 cut(s) 290, 881
MspI CCGG 7 cut(s) 377, 384, 489, 506, 774, 816, 985
MspR9I CCNGG 4 cut(s) 489, 681, 816, 985
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 788
MwoI GCNNNNNNNGC 4 cut(s) 449, 478, 864, 958
NciI CCSGG 3 cut(s) 489, 816, 985
NdeI CATATG 1 cut(s) 433
NdeII GATC 6 cut(s) 138, 502, 522, 642, 664, 789
NlaIII CATG 5 cut(s) 220, 867, 880, 1000, 1024
NlaIV GGNNCC 4 cut(s) 241, 375, 504, 874
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 1 cut(s) 220
NspV TTCGAA 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 558
PceI AGGCCT 1 cut(s) 269
PciI ACATGT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PfeI GAWTC 2 cut(s) 428, 527
PkrI GCNGC 4 cut(s) 331, 393, 416, 976
Ple19I CGATCG 1 cut(s) 792
PleI GAGTC 6 cut(s) 374, 500, 644, 703, 716, 934
PmaCI CACGTG 1 cut(s) 992
PmlI CACGTG 1 cut(s) 992
PpsI GAGTC 6 cut(s) 374, 500, 644, 703, 716, 934
Ppu21I YACGTR 2 cut(s) 613, 992
PscI ACATGT 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 992
Psp1406I AACGTT 2 cut(s) 355, 363
Psp6I CCWGG 1 cut(s) 679
PspCI CACGTG 1 cut(s) 992
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 4 cut(s) 241, 375, 504, 874
PspPI GGNCC 3 cut(s) 515, 605, 1052
PspXI VCTCGAGB 1 cut(s) 558
PsuI RGATCY 1 cut(s) 502
PvuI CGATCG 1 cut(s) 792
RgaI GCGATCGC 1 cut(s) 792
RsaI GTAC 3 cut(s) 308, 551, 716
RsaNI GTAC 3 cut(s) 307, 550, 715
RseI CAYNNNNRTG 2 cut(s) 290, 881
SaqAI TTAA 4 cut(s) 192, 276, 288, 581
SatI GCNGC 4 cut(s) 330, 392, 415, 975
Sau3AI GATC 6 cut(s) 138, 502, 522, 642, 664, 789
Sau96I GGNCC 3 cut(s) 515, 605, 1052
SchI GAGTC 6 cut(s) 374, 501, 645, 704, 716, 935
ScrFI CCNGG 4 cut(s) 489, 681, 816, 985
SduI GDGCHC 3 cut(s) 346, 834, 963
SfaAI GCGATCGC 1 cut(s) 792
SfaNI GCATC 1 cut(s) 841
Sfr274I CTCGAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 805
SgfI GCGATCGC 1 cut(s) 792
SinI GGWCC 2 cut(s) 605, 1052
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 290, 881
SmlI CTYRAG 2 cut(s) 345, 558
SmoI CTYRAG 2 cut(s) 345, 558
Sse9I AATT 2 cut(s) 91, 965
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 7 cut(s) 452, 513, 758, 942, 952, 956, 974
SspMI CTAG 1 cut(s) 1096
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 4 cut(s) 487, 679, 814, 983
TaaI ACNGT 3 cut(s) 406, 466, 910
TaiI ACGT 5 cut(s) 358, 366, 615, 994, 1071
TaqI TCGA 8 cut(s) 69, 272, 495, 525, 559, 762, 805, 980
TaqII GACCGA 1 cut(s) 520
TasI AATT 2 cut(s) 91, 965
TatI WGTACW 2 cut(s) 306, 714
TauI GCSGC 1 cut(s) 977
TfiI GAWTC 2 cut(s) 428, 527
Tru1I TTAA 4 cut(s) 192, 276, 288, 581
Tru9I TTAA 4 cut(s) 192, 276, 288, 581
TscAI CASTG 2 cut(s) 411, 714
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 329, 391, 414
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 420, 474
TspGWI ACGGA 1 cut(s) 832
TspRI CASTG 2 cut(s) 411, 714
VpaK11BI GGWCC 2 cut(s) 605, 1052
XapI RAATTY 1 cut(s) 965
XceI RCATGY 1 cut(s) 220
XhoI CTCGAG 1 cut(s) 558
XspI CTAG 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.