Rorug06G0282000

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009179
Physical Location & Seq
Reverse (-)
42974310 .. 42975651
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0282000.1

Sequence Viewer

Length: 1083 bp
ATGGCTTTGAGCAACAAAAAGGTCTCCACCTCAGCCCCTCAATGGAAATATCATGTTTTCTTGAGTTTTAGGGGTGAAGACACCCGCAAGGATTTTACAGATCATCTCTTTGAGAAATTGAAGGAGCGAGCAATCAATGCTTTCAGGGATGACCCAAATCTCAAAAGAGGGACTACTATCGATAAAGAGCTCATGCTGGCAATTAAACAGTCGAGGTTTTCAATTGTCATTTTTTCGAAAAACTATGCTTCTTCTGATTGGTGCTTGCTTGAACTTGCACATATTGTTCGATGCATGAAAGAGGATGGTAGAATCATGCCAATCTTTTATGGCGTAGAACCTAGACATGTACGTCATCAAACGGGAACTTTTGCAAAACATTTCACCGAGCATGAAAAGAAGTTGGGAAAAGATGGAGAGAAAGTGCGCGAGTGGAGAGATGCATTGACAAAAGTGGGTAATCTTGCTGGCTGGGATTCAGAGAATTATAGGTACAACACAGCGCTTATCAAAGAAATTGTTGATGTAGTGTGGAAGAAAGTTAATCCTACATTTCCGGGGGAAGATCCTACACAGAAATCGGTCGGACTTGGTAAAGTCAACAAAATAGTTTCGCTTTTGAATCTAGACTCAAATGATATTCGGCTCGTAGGAATATGGGGAGCGGATGAGATAGCCATTAAAACAACTGCTGACTTAGTCCATAGTAGTATTTCTGATCATTTTGAAGTAAGCAGCTTTCTTAGAGAAATCAGAAAGGTTTCCAAAAGTAAGAGTTTAGTTTATCTTCAAGCTCGGCTTCTGGAACAACTAAACGGAAGGTACGTACCAGTTTCAGATGTTTATGAGGGAAAGAATATGACAAAGAATTGTGTACGAAATAAAAAGGTTCTTCGCATTCTCGATGATGTGGATCAATCAGACCAAATAGAAATCCTGGGTGGAGAGAAGGACTGGTATGGCAATGGTAGCAGAATCATCATTACATCTATGAATCGAGATCTGCTAGTAGAACATGGCATAGAAGAACCATATTTGGTGGAGGGATTGAACAAAGATGAAGCTCTTCAGCTCTACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

360

Amino Acids

41.32

Weight (kDa)

8.5

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 188 2e-54 TIR domain
TIR_2 PF13676 19 - 114 1.1e-14 TIR domain
NB-ARC PF00931 201 - 359 1.6e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32460 AT4G32460 AT4G32460 AT4G32460 AT5G11420 AT5G25460
fragaria_vesca FvH4_2g29680
malus_domestica MD08G1143200.v1.1 MD15G1120000.v1.1
prunus_persica Prupe.1G472800_v2.0.a1
pyrus_communis pycom08g12190 pycom15g10810
rosa_chinensis RchiOBHm_Chr6g0298661
rosa_laevigata RLG00000011495
rosa_multiflora Rmu_sc0008099.1_g000008
rosa_roxburghii Rroxscaffold_7G00169440
rosa_rugosa Rorug06G0281900 Rorug06G0282000
rosa_samantha Rh6AG393500 Rh6BG401300 Rh6DG393900
rosa_wichuraiana Rw6G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 351
AccBSI CCGCTC 1 cut(s) 665
AccII CGCG 1 cut(s) 429
AciI CCGC 2 cut(s) 85, 665
AclWI GGATC 2 cut(s) 560, 921
AcuI CTGAAG 1 cut(s) 1052
AfaI GTAC 5 cut(s) 351, 494, 824, 828, 876
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 7 cut(s) 121, 222, 272, 622, 728, 791, 1051
AjnI CCWGG 1 cut(s) 936
AluBI AGCT 5 cut(s) 190, 738, 794, 1064, 1072
AluI AGCT 5 cut(s) 190, 738, 794, 1064, 1072
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 2 cut(s) 560, 921
Aor51HI AGCGCT 1 cut(s) 504
ApeKI GCWGC 1 cut(s) 735
Asp700I GAANNNNTTC 2 cut(s) 760, 1065
AspLEI GCGC 2 cut(s) 429, 505
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 2 cut(s) 86, 376
AsuII TTCGAA 1 cut(s) 236
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 747
BccI CCATC 2 cut(s) 299, 407
BciT130I CCWGG 1 cut(s) 938
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 3 cut(s) 342, 626, 1007
BfmI CTRYAG 1 cut(s) 1075
BfoI RGCGCY 1 cut(s) 506
BglII AGATCT 1 cut(s) 1000
BisI GCNGC 1 cut(s) 736
BlsI GCNGC 1 cut(s) 737
Bme1390I CCNGG 2 cut(s) 558, 938
BmrFI CCNGG 2 cut(s) 558, 938
BmsI GCATC 2 cut(s) 281, 430
BpiI GAAGAC 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 558
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 826
BsaBI GATNNNNATC 1 cut(s) 912
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 557, 937
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 830, 959
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 1 cut(s) 938
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 557, 937
BseGI GGATG 3 cut(s) 154, 310, 673
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 830, 959
BseXI GCAGC 1 cut(s) 747
BseYI CCCAGC 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 585
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 897
Bso31I GGTCTC 1 cut(s) 28
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 5 cut(s) 100, 565, 718, 913, 1000
BspACI CCGC 2 cut(s) 85, 665
BspCNI CTCAG 1 cut(s) 44
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 1 cut(s) 429
BspPI GGATC 2 cut(s) 560, 921
BspQI GCTCTTC 1 cut(s) 1071
BspT104I TTCGAA 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 28
BsrBI CCGCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 2 cut(s) 830, 959
BssECI CCNNGG 2 cut(s) 557, 937
BssMI GATC 5 cut(s) 100, 565, 718, 913, 1000
Bst2UI CCWGG 1 cut(s) 938
Bst4CI ACNGT 2 cut(s) 210, 1079
Bst6I CTCTTC 1 cut(s) 1071
BstAPI GCANNNNNTGC 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 826
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 129, 198, 266, 469
BstDEI CTNAG 3 cut(s) 31, 697, 743
BstF5I GGATG 3 cut(s) 154, 310, 673
BstFNI CGCG 1 cut(s) 429
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 2 cut(s) 429, 505
BstKTI GATC 5 cut(s) 103, 568, 721, 916, 1003
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 5 cut(s) 100, 565, 718, 913, 1000
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 2 cut(s) 137, 969
BstNI CCWGG 1 cut(s) 938
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 556, 936
BstSFI CTRYAG 1 cut(s) 1075
BstSNI TACGTA 1 cut(s) 826
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 747
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 565, 1000
BstYI RGATCY 2 cut(s) 565, 1000
Bsu15I ATCGAT 1 cut(s) 180
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 3 cut(s) 154, 310, 673
Cac8I GCNNGC 4 cut(s) 129, 198, 266, 469
CfoI GCGC 2 cut(s) 429, 505
ClaI ATCGAT 1 cut(s) 180
Csp6I GTAC 5 cut(s) 350, 493, 823, 827, 875
CviAII CATG 7 cut(s) 53, 193, 295, 316, 347, 392, 1016
CviQI GTAC 5 cut(s) 350, 493, 823, 827, 875
DdeI CTNAG 3 cut(s) 31, 697, 743
DpnI GATC 5 cut(s) 102, 567, 720, 915, 1002
DpnII GATC 5 cut(s) 100, 565, 718, 913, 1000
DrdI GACNNNNNNGTC 1 cut(s) 351
DseDI GACNNNNNNGTC 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 1071
EarI CTCTTC 1 cut(s) 1071
Ecl136II GAGCTC 1 cut(s) 190
Eco105I TACGTA 1 cut(s) 826
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 28
Eco47III AGCGCT 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 1052
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 936
EcoT22I ATGCAT 2 cut(s) 296, 445
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 7 cut(s) 56, 196, 298, 319, 350, 395, 1019
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 1 cut(s) 184
FatI CATG 7 cut(s) 52, 192, 294, 315, 346, 391, 1015
FauI CCCGC 1 cut(s) 92
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 1 cut(s) 736
FokI GGATG 3 cut(s) 161, 317, 680
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 736
FspBI CTAG 3 cut(s) 342, 626, 1007
GlaI GCGC 2 cut(s) 428, 504
GluI GCNGC 1 cut(s) 736
GsaI CCCAGC 1 cut(s) 475
HaeII RGCGCY 1 cut(s) 506
HapII CCGG 1 cut(s) 557
HhaI GCGC 2 cut(s) 429, 505
Hin1II CATG 7 cut(s) 56, 196, 298, 319, 350, 395, 1019
Hin6I GCGC 2 cut(s) 427, 503
HinP1I GCGC 2 cut(s) 427, 503
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 6 cut(s) 312, 476, 622, 629, 975, 994
HpaII CCGG 1 cut(s) 557
HphI GGTGA 2 cut(s) 86, 376
Hpy166II GTNNAC 2 cut(s) 601, 875
Hpy188I TCNGA 7 cut(s) 256, 481, 587, 718, 755, 838, 922
Hpy188III TCNNGA 5 cut(s) 61, 626, 803, 902, 998
Hpy8I GTNNAC 2 cut(s) 601, 875
HpyAV CCTTC 3 cut(s) 115, 813, 943
HpyCH4III ACNGT 2 cut(s) 210, 1079
HpyCH4IV ACGT 2 cut(s) 352, 825
HpyCH4V TGCA 4 cut(s) 278, 294, 374, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 969
HpyF3I CTNAG 3 cut(s) 31, 697, 743
HpySE526I ACGT 2 cut(s) 352, 825
Hsp92II CATG 7 cut(s) 56, 196, 298, 319, 350, 395, 1019
HspAI GCGC 2 cut(s) 427, 503
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 5 cut(s) 100, 565, 718, 913, 1000
LguI GCTCTTC 1 cut(s) 1071
LmnI GCTCC 2 cut(s) 124, 662
Lsp1109I GCAGC 1 cut(s) 747
LweI GCATC 2 cut(s) 281, 430
MaeI CTAG 3 cut(s) 342, 626, 1007
MaeII ACGT 2 cut(s) 352, 825
MalI GATC 5 cut(s) 102, 567, 720, 915, 1002
MbiI CCGCTC 1 cut(s) 665
MboI GATC 5 cut(s) 100, 565, 718, 913, 1000
MboII GAAGA 8 cut(s) 89, 243, 547, 575, 779, 884, 1037, 1058
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 2 cut(s) 565, 1000
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 6 cut(s) 116, 201, 222, 484, 516, 868
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 7 cut(s) 40, 48, 161, 207, 295, 841, 1036
Mph1103I ATGCAT 2 cut(s) 296, 445
MroXI GAANNNNTTC 2 cut(s) 760, 1065
MseI TTAA 3 cut(s) 204, 543, 681
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 2 cut(s) 558, 938
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 1 cut(s) 938
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 137, 969
NciI CCSGG 1 cut(s) 558
NdeII GATC 5 cut(s) 100, 565, 718, 913, 1000
NlaIII CATG 7 cut(s) 56, 196, 298, 319, 350, 395, 1019
NmeAIII GCCGAG 1 cut(s) 775
NsiI ATGCAT 2 cut(s) 296, 445
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 1 cut(s) 236
PciI ACATGT 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PctI GAATGC 1 cut(s) 897
PdmI GAANNNNTTC 2 cut(s) 760, 1065
PfeI GAWTC 5 cut(s) 312, 476, 622, 975, 994
PflFI GACNNNGTC 1 cut(s) 698
PkrI GCNGC 1 cut(s) 737
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Ppu21I YACGTR 1 cut(s) 826
PscI ACATGT 1 cut(s) 346
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 936
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 936
PsuI RGATCY 2 cut(s) 565, 1000
PsyI GACNNNGTC 1 cut(s) 698
RsaI GTAC 5 cut(s) 351, 494, 824, 828, 876
RsaNI GTAC 5 cut(s) 350, 493, 823, 827, 875
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 1071
SaqAI TTAA 3 cut(s) 204, 543, 681
SatI GCNGC 1 cut(s) 736
Sau3AI GATC 5 cut(s) 100, 565, 718, 913, 1000
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 558, 938
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 2 cut(s) 281, 430
SfcI CTRYAG 1 cut(s) 1075
SfuI TTCGAA 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SnaBI TACGTA 1 cut(s) 826
Sse9I AATT 6 cut(s) 116, 201, 222, 484, 516, 868
SsiI CCGC 2 cut(s) 85, 665
SspMI CTAG 3 cut(s) 342, 626, 1007
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 2 cut(s) 556, 936
TaaI ACNGT 2 cut(s) 210, 1079
TaiI ACGT 2 cut(s) 355, 828
TaqI TCGA 6 cut(s) 180, 212, 236, 289, 903, 997
TaqII GACCGA 1 cut(s) 571
TasI AATT 6 cut(s) 116, 201, 222, 484, 516, 868
TfiI GAWTC 5 cut(s) 312, 476, 622, 975, 994
Tru1I TTAA 3 cut(s) 204, 543, 681
Tru9I TTAA 3 cut(s) 204, 543, 681
TseI GCWGC 1 cut(s) 735
TspDTI ATGAA 4 cut(s) 311, 408, 1007, 1074
TspGWI ACGGA 1 cut(s) 831
Tth111I GACNNNGTC 1 cut(s) 698
XbaI TCTAGA 1 cut(s) 625
XceI RCATGY 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 760, 1065
XspI CTAG 3 cut(s) 342, 626, 1007
Zsp2I ATGCAT 2 cut(s) 296, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.